LOCUS       NM_021132               3519 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens protein phosphatase 3 catalytic subunit beta (PPP3CB),
            transcript variant 2, mRNA.
ACCESSION   NM_021132
VERSION     NM_021132.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Gazzeri,S., Zubchuk,N., Montaudon,E., Nemati,F., Huot-Marchand,S.,
            Berardi,G., Pucciarelli,A., Dib,Y., Nerini,D., Oddou,C., Pezet,M.,
            David-Boudet,L., Ardin,C., de Fraipont,F., Maraver,A., Girard,N.,
            Decaudin,D., Toffart,A.C. and Eymin,B.
  TITLE     PPP3CB overexpression mediates EGFR TKI resistance in lung tumors
            via calcineurin/MEK/ERK signaling
  JOURNAL   Life Sci Alliance 7 (12), e202402873 (2024)
   PUBMED   39353739
  REMARK    GeneRIF: PPP3CB overexpression mediates EGFR TKI resistance in lung
            tumors via calcineurin/MEK/ERK signaling.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Viard,J., Loe-Mie,Y., Daudin,R., Khelfaoui,M., Plancon,C.,
            Boland,A., Tejedor,F., Huganir,R.L., Kim,E., Kinoshita,M., Liu,G.,
            Haucke,V., Moncion,T., Yu,E., Hindie,V., Blehaut,H., Mircher,C.,
            Herault,Y., Deleuze,J.F., Rain,J.C., Simonneau,M. and
            Lepagnol-Bestel,A.M.
  TITLE     Chr21 protein-protein interactions: enrichment in proteins involved
            in intellectual disability, autism, and late-onset Alzheimer's
            disease
  JOURNAL   Life Sci Alliance 5 (12), e202101205 (2022)
   PUBMED   35914814
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3519)
  AUTHORS   Miyata,H., Oura,S., Morohoshi,A., Shimada,K., Mashiko,D., Oyama,Y.,
            Kaneda,Y., Matsumura,T., Abbasi,F. and Ikawa,M.
  TITLE     SPATA33 localizes calcineurin to the mitochondria and regulates
            sperm motility in mice
  JOURNAL   Proc Natl Acad Sci U S A 118 (35) (2021)
   PUBMED   34446558
REFERENCE   4  (bases 1 to 3519)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 3519)
  AUTHORS   Kumar,S., Jain,A., Choi,S.W., da Silva,G.P.D., Allers,L.,
            Mudd,M.H., Peters,R.S., Anonsen,J.H., Rusten,T.E., Lazarou,M. and
            Deretic,V.
  TITLE     Mammalian Atg8 proteins and the autophagy factor IRGM control mTOR
            and TFEB at a regulatory node critical for responses to pathogens
  JOURNAL   Nat Cell Biol 22 (8), 973-985 (2020)
   PUBMED   32753672
  REMARK    Erratum:[Nat Cell Biol. 2020 Oct;22(10):1286. doi:
            10.1038/s41556-020-0574-0.. PMID: 32788721]
REFERENCE   6  (bases 1 to 3519)
  AUTHORS   Chen,M.X., Chen,Y.H. and Cohen,P.T.
  TITLE     Polymerase chain reactions using Saccharomyces, Drosophila and
            human DNA predict a large family of protein serine/threonine
            phosphatases
  JOURNAL   FEBS Lett 306 (1), 54-58 (1992)
   PUBMED   1321058
REFERENCE   7  (bases 1 to 3519)
  AUTHORS   Giri,P.R., Higuchi,S. and Kincaid,R.L.
  TITLE     Chromosomal mapping of the human genes for the calmodulin-dependent
            protein phosphatase (calcineurin) catalytic subunit
  JOURNAL   Biochem Biophys Res Commun 181 (1), 252-258 (1991)
   PUBMED   1659808
REFERENCE   8  (bases 1 to 3519)
  AUTHORS   McPartlin,A.E., Barker,H.M. and Cohen,P.T.
  TITLE     Identification of a third alternatively spliced cDNA encoding the
            catalytic subunit of protein phosphatase 2B beta
  JOURNAL   Biochim Biophys Acta 1088 (2), 308-310 (1991)
   PUBMED   1848109
REFERENCE   9  (bases 1 to 3519)
  AUTHORS   Guerini,D. and Klee,C.B.
  TITLE     Cloning of human calcineurin A: evidence for two isozymes and
            identification of a polyproline structural domain
  JOURNAL   Proc Natl Acad Sci U S A 86 (23), 9183-9187 (1989)
   PUBMED   2556704
REFERENCE   10 (bases 1 to 3519)
  AUTHORS   Guerini,D., Krinks,M.H., Sikela,J.M., Hahn,W.E. and Klee,C.B.
  TITLE     Isolation and sequence of a cDNA clone for human calcineurin B, the
            Ca2+-binding subunit of the Ca2+/calmodulin-stimulated protein
            phosphatase
  JOURNAL   DNA 8 (9), 675-682 (1989)
   PUBMED   2558868
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA854064.1, BC035464.1, BC028049.1 and BX114972.1.
            
            On Jun 2, 2019 this sequence version replaced NM_021132.3.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the coding region, compared to variant 1. It encodes
            isoform b which is shorter than isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC035464.1, M29551.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000360663.10/ ENSP00000353881.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                DA854064.1         1-42
            43-1537             BC035464.1         1-1495
            1538-3147           BC028049.1         1529-3138
            3148-3519           BX114972.1         19-390              c
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q22.2"
     gene            1..3519
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /note="protein phosphatase 3 catalytic subunit beta"
                     /db_xref="GeneID:5532"
                     /db_xref="HGNC:HGNC:9315"
                     /db_xref="MIM:114106"
     exon            1..220
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /inference="alignment:Splign:2.1.0"
     CDS             136..1710
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /EC_number="3.1.3.16"
                     /note="isoform b is encoded by transcript variant 2;
                     calcineurin A beta; CAM-PRP catalytic subunit; calcineurin
                     A2; protein phosphatase 3, catalytic subunit, beta
                     isozyme; protein phosphatase from PCR fragment H32; CNA
                     beta"
                     /codon_start=1
                     /product="serine/threonine-protein phosphatase 2B
                     catalytic subunit beta isoform isoform b"
                     /protein_id="NP_066955.1"
                     /db_xref="CCDS:CCDS7328.1"
                     /db_xref="GeneID:5532"
                     /db_xref="HGNC:HGNC:9315"
                     /db_xref="MIM:114106"
                     /translation="MAAPEPARAAPPPPPPPPPPPGADRVVKAVPFPPTHRLTSEEVF
                     DLDGIPRVDVLKNHLVKEGRVDEEIALRIINEGAAILRREKTMIEVEAPITVCGDIHG
                     QFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWVLKILYPSTLFLLRGNH
                     ECRHLTEYFTFKQECKIKYSERVYEACMEAFDSLPLAALLNQQFLCVHGGLSPEIHTL
                     DDIRRLDRFKEPPAFGPMCDLLWSDPSEDFGNEKSQEHFSHNTVRGCSYFYNYPAVCE
                     FLQNNNLLSIIRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYE
                     NNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLSICSDDELMTEGED
                     QFDGSAAARKEIIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLAGGR
                     QTLQSATVEAIEAEKAIRGFSPPHRICSFEEAKGLDRINERMPPRKDAVQQDGFNSLN
                     TAHATENHGTGNHTAQ"
     exon            221..421
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            422..546
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            547..658
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            659..804
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            805..944
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1022
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1117
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1243
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1321
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1403
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1501
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1531
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..3519
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3117..3122
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /note="hexamer: AATAAA"
     polyA_site      3147
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /note="major polyA site"
     regulatory      3500..3505
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
                     /note="hexamer: AAGAAA"
     polyA_site      3519
                     /gene="PPP3CB"
                     /gene_synonym="CALNA2; CALNB; CNA2; PP2Bbeta"
ORIGIN      
        1 atgccggtct tagcccctct gtagggaaag agggtccgcc atgttccccg gcggcgccgc
       61 cgcttggctc tggtagccgc cgcccccgcc cccaaccccg cccggcccag agcctagccg
      121 agccccgggc ccagcatggc cgccccggag ccggcccggg ctgcaccgcc cccacccccg
      181 cccccgccgc cccctcccgg ggctgaccgc gtcgtcaaag ctgtcccttt ccccccaaca
      241 catcgcttga catctgaaga agtatttgat ttggatggga tacccagggt tgatgttctg
      301 aagaaccact tggtgaaaga aggtcgagta gatgaagaaa ttgcgcttag aattatcaat
      361 gagggtgctg ccatccttcg gagagagaaa accatgatag aagtagaagc tccaatcaca
      421 gtgtgtggtg acatccatgg ccaatttttt gatctgatga aactttttga agtaggagga
      481 tcacctgcta atacacgata cctttttctt ggcgattatg tggacagagg ttattttagt
      541 atagagtgtg tcttatattt atgggttctg aagattctat acccaagcac attatttctt
      601 ctgagaggca accatgaatg cagacacctt actgaatatt ttacctttaa gcaggaatgt
      661 aaaattaagt attcggaaag agtctatgaa gcttgtatgg aagcttttga tagtttgcct
      721 cttgctgcac ttttaaacca acagtttctt tgtgttcatg gtggactttc accagaaata
      781 cacacactgg atgatattag gagattagat agattcaaag agccacctgc atttggacca
      841 atgtgtgact tgttatggtc cgatccttct gaagattttg gaaatgaaaa atcacaggaa
      901 cattttagtc acaatacagt tcgaggatgt tcttattttt ataactatcc agcagtgtgt
      961 gaatttttgc aaaacaataa tttgttatcg attattagag ctcatgaagc tcaagatgca
     1021 ggctatagaa tgtacagaaa aagtcaaact acagggttcc cttcattaat aacaattttt
     1081 tcggcaccta attacttaga tgtctacaat aataaagctg ctgtattaaa gtatgaaaat
     1141 aatgtgatga atattcgaca gtttaactgt tctccacatc cttactggtt gcctaatttt
     1201 atggatgtct tcacgtggtc tttaccgttt gttggagaaa aagtgacaga aatgttggta
     1261 aatgttctga gtatttgctc tgatgatgaa ctaatgactg aaggtgaaga ccagtttgat
     1321 ggttcagctg cagcccggaa agaaatcata agaaacaaaa ttcgagcaat tggcaagatg
     1381 gcaagagtct tctctgttct cagggaggag agtgaaagtg tgctgacact caagggcctg
     1441 actcccacag ggatgttgcc tagtggagtg ttagctggag gacggcagac cctgcaaagt
     1501 gccacagttg aggctattga ggctgaaaaa gcaatacgag gattctctcc accacataga
     1561 atctgcagtt ttgaagaggc aaagggtttg gataggatca atgagagaat gccacctcgg
     1621 aaagatgctg tacagcaaga tggtttcaat tctctgaaca ccgcacatgc cactgagaac
     1681 cacgggacgg gcaaccatac tgcccagtga cccactactt cccagggact ctcacatctc
     1741 gggccccaaa tggacagatc acccgaggag ctggaggggt cggccaagct gactgtaaat
     1801 ttcacagtct ctctgaagaa accattgtgc ttctgagacc ctagccccct tcctggatgg
     1861 aggcttgagg gccctgggac atgtgctatc tgataagatt gggtcatcgc tgccaaggtg
     1921 gagagcagtg agcaaggggc ttggggcaat ttccagtgga gggcatccac acctccattt
     1981 tatgcttgtg gttcacacat ttaagtttac aaatcagatt tcttttcccc ttcagtagaa
     2041 ttagattttg tttttcaatc atgatttcaa atgcaatcct aagagctaat gtggactttt
     2101 ctttttccat gaaatgtctt taaaggatga attagcatgg tcttaaaata catttctgag
     2161 gttactagct gtattttgaa ttgtgagcaa aatgccgaga aacccagttg gcatttatac
     2221 aaaatgttga cctcaggtct atagttctta aatgtggcta attctgtaac atagtcttgg
     2281 tattttttaa ttatgaatgc atatcctatt tccaggcagg ctctcttact tgaacacaaa
     2341 tccaaaaact aatttagagt cttttttgcc cagatctttt aagacttaca ccccagagat
     2401 ttaagaagaa aacctctaaa tttcaaaatt atgaagaatt acagaattac tcatttaagg
     2461 tactttaaaa gaagtttgta cattgtcaaa gtaaatttta attcaaatca tgtctgtaaa
     2521 acttgacgta ttttgtgtat gcatgttttc attttgcaaa tatttaatat atagacctat
     2581 gatgtacagg tacgacatgt ataggttacc tagatgttat gagaaatttt agtttattgt
     2641 gagtactcaa gttgcttaga gagccaccag ggtgatttgc tgctggcttt ctatcatttt
     2701 tatgttttaa tgcaaaggaa attttaaaat gttctggaag tgtttttgat taagcaatgc
     2761 agcctagaag caatggttct gttcaatcat tcagatgtta gtggaagcat aaaagtcaag
     2821 actgcatgtt gaaacctttc ttttgatagt tactgaactg cttggttaaa ctaaatggaa
     2881 ccatgtgcta atttttcaca attattgacc tgtattgatt gccactgtag tttggtattt
     2941 ccctttactt tggtggcctg cttccctcat gccctggaat acaactcaga gctccaggca
     3001 gcggaaccat ctattgtttt gtttgccaga aagtgcaccc tgtatggtct cctgtctaag
     3061 ttggaaatat tatgcatgtg caggactatt cgagtatttt ataaacagta gcacacaata
     3121 aattccatgc atgggccgct gctcctatct ctgtgttggg ttttatttgg aagatgcaat
     3181 ctgatttgtc cttttgatgc aaatcagaaa atcctgttac tagagctggg atgtcctccg
     3241 gagattatct cgtggatagt tcatggtaat ttgattaatt aaattcttta taaattttgc
     3301 cttaaaaaaa acttttgtta tatacttgtt ttacatgagc attagtaact gagcactaga
     3361 ggactttgaa tgcctactgt aggcctccta agtctaatat ttaagatcac tgtttattgt
     3421 cttttaattg aaagaaaata tgttattgtc tagaattttg ttatagtggt attgggaatt
     3481 tactgggtgt tctaacaata agaaaaatat tagtgataa
//
