LOCUS       NM_032595               4212 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens protein phosphatase 1 regulatory subunit 9B (PPP1R9B),
            mRNA.
ACCESSION   NM_032595
VERSION     NM_032595.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Kim,M., Park,J., Bouhaddou,M., Kim,K., Rojc,A., Modak,M.,
            Soucheray,M., McGregor,M.J., O'Leary,P., Wolf,D., Stevenson,E.,
            Foo,T.K., Mitchell,D., Herrington,K.A., Munoz,D.P., Tutuncuoglu,B.,
            Chen,K.H., Zheng,F., Kreisberg,J.F., Diolaiti,M.E., Gordan,J.D.,
            Coppe,J.P., Swaney,D.L., Xia,B., van 't Veer,L., Ashworth,A.,
            Ideker,T. and Krogan,N.J.
  TITLE     A protein interaction landscape of breast cancer
  JOURNAL   Science 374 (6563), eabf3066 (2021)
   PUBMED   34591612
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 4212)
  AUTHORS   Verdugo-Sivianes,E.M., Rojas,A.M., Munoz-Galvan,S., Otero-Albiol,D.
            and Carnero,A.
  TITLE     Mutation of SPINOPHILIN (PPP1R9B) found in human tumors promotes
            the tumorigenic and stemness properties of cells
  JOURNAL   Theranostics 11 (7), 3452-3471 (2021)
   PUBMED   33537097
  REMARK    GeneRIF: Mutation of SPINOPHILIN (PPP1R9B) found in human tumors
            promotes the tumorigenic and stemness properties of cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4212)
  AUTHORS   Chen,Y., Booth,C., Wang,H., Wang,R.X., Terzi,D., Zachariou,V.,
            Jiao,K., Zhang,J. and Wang,Q.
  TITLE     Effective Attenuation of Adenosine A1R Signaling by Neurabin
            Requires Oligomerization of Neurabin
  JOURNAL   Mol Pharmacol 92 (6), 630-639 (2017)
   PUBMED   28954816
  REMARK    GeneRIF: homo-oligomerization of neurabin is required for
            stabilizing RGS4 on the plasma membrane to attenuate A1R signaling
REFERENCE   5  (bases 1 to 4212)
  AUTHORS   Hiday,A.C., Edler,M.C., Salek,A.B., Morris,C.W., Thang,M.,
            Rentz,T.J., Rose,K.L., Jones,L.M. and Baucum,A.J. 2nd.
  TITLE     Mechanisms and Consequences of Dopamine Depletion-Induced
            Attenuation of the Spinophilin/Neurofilament Medium Interaction
  JOURNAL   Neural Plast 2017, 4153076 (2017)
   PUBMED   28634551
  REMARK    GeneRIF: Study determined that spinophilin binding to neurofilament
            medium required overexpression of the catalytic subunit of protein
            kinase A and was decreased by cyclin-dependent protein kinase 5.
REFERENCE   6  (bases 1 to 4212)
  AUTHORS   Stephens,D.J. and Banting,G.
  TITLE     Direct interaction of the trans-Golgi network membrane protein,
            TGN38, with the F-actin binding protein, neurabin
  JOURNAL   J Biol Chem 274 (42), 30080-30086 (1999)
   PUBMED   10514494
REFERENCE   7  (bases 1 to 4212)
  AUTHORS   Smith,F.D., Oxford,G.S. and Milgram,S.L.
  TITLE     Association of the D2 dopamine receptor third cytoplasmic loop with
            spinophilin, a protein phosphatase-1-interacting protein
  JOURNAL   J Biol Chem 274 (28), 19894-19900 (1999)
   PUBMED   10391935
REFERENCE   8  (bases 1 to 4212)
  AUTHORS   Hsieh-Wilson,L.C., Allen,P.B., Watanabe,T., Nairn,A.C. and
            Greengard,P.
  TITLE     Characterization of the neuronal targeting protein spinophilin and
            its interactions with protein phosphatase-1
  JOURNAL   Biochemistry 38 (14), 4365-4373 (1999)
   PUBMED   10194355
REFERENCE   9  (bases 1 to 4212)
  AUTHORS   Burnett,P.E., Blackshaw,S., Lai,M.M., Qureshi,I.A., Burnett,A.F.,
            Sabatini,D.M. and Snyder,S.H.
  TITLE     Neurabin is a synaptic protein linking p70 S6 kinase and the
            neuronal cytoskeleton
  JOURNAL   Proc Natl Acad Sci U S A 95 (14), 8351-8356 (1998)
   PUBMED   9653190
REFERENCE   10 (bases 1 to 4212)
  AUTHORS   Allen,P.B., Ouimet,C.C. and Greengard,P.
  TITLE     Spinophilin, a novel protein phosphatase 1 binding protein
            localized to dendritic spines
  JOURNAL   Proc Natl Acad Sci U S A 94 (18), 9956-9961 (1997)
   PUBMED   9275233
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC002401.2, BF448737.1,
            AJ401189.1, ABBA01041227.1, AL713642.1 and BM991997.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_032595.4.
            
            Summary: This gene encodes a scaffold protein that functions as a
            regulatory subunit of protein phosphatase 1a. Expression of this
            gene is particularly high in dendritic spines, suggesting that the
            encoded protein may play a role in receiving signals from the
            central nervous system. The encoded protein has putative tumor
            suppressor function and decreased expression has been observed in
            tumors. [provided by RefSeq, Feb 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL713642.1, SRR3476690.867667.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000612501.2/ ENSP00000478767.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-160               AC002401.2         4874-5033
            161-164             BF448737.1         480-483             c
            165-635             AJ401189.1         1-471
            636-659             ABBA01041227.1     81540-81563         c
            660-1353            AJ401189.1         490-1183
            1354-3779           AL713642.1         1-2426
            3780-4212           BM991997.1         21-453              c
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.33"
     gene            1..4212
                     /gene="PPP1R9B"
                     /gene_synonym="PPP1R6; PPP1R9; SPINO; Spn"
                     /note="protein phosphatase 1 regulatory subunit 9B"
                     /db_xref="GeneID:84687"
                     /db_xref="HGNC:HGNC:9298"
                     /db_xref="MIM:603325"
     exon            1..1535
                     /gene="PPP1R9B"
                     /gene_synonym="PPP1R6; PPP1R9; SPINO; Spn"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..2618
                     /gene="PPP1R9B"
                     /gene_synonym="PPP1R6; PPP1R9; SPINO; Spn"
                     /note="protein phosphatase 1, regulatory subunit 9B,
                     spinophilin; protein phosphatase 1, regulatory (inhibitor)
                     subunit 9B; neurabin-II"
                     /codon_start=1
                     /product="neurabin-2"
                     /protein_id="NP_115984.3"
                     /db_xref="CCDS:CCDS74102.1"
                     /db_xref="GeneID:84687"
                     /db_xref="HGNC:HGNC:9298"
                     /db_xref="MIM:603325"
                     /translation="MMKTEPRGPGGPLRSASPHRSAYEAGIQALKPPDAPGPDEAPKG
                     AHHKKYGSNVHRIKSMFLQMGTTAGPSGEAGGGAGLAEAPRASERGVRLSLPRASSLN
                     ENVDHSALLKLGTSVSERVSRFDSKPAPSAQPAPPPHPPSRLQETRKLFERSAPAAAG
                     GDKEAAARRLLRQERAGLQDRKLDVVVRFNGSTEALDKLDADAVSPTVSQLSAVFEKA
                     DSRTGLHRGPGLPRAAGVPQVNSKLVSKRSRVFQPPPPPPPAPSGDAPAEKERCPAGQ
                     QPPQHRVAPARPPPKPREVRKIKPVEVEESGESEAESAPGEVIQAEVTVHAALENGST
                     VATAASPAPEEPKAQAAPEKEAAAVAPPERGVGNGRAPDVAPEEVDESKKEDFSEADL
                     VDVSAYSGLGEDSAGSALEEDDEDDEEDGEPPYEPESGCVEIPGLSEEEDPAPSRKIH
                     FSTAPIQVFSTYSNEDYDRRNEDVDPMAASAEYELEKRVERLELFPVELEKDSEGLGI
                     SIIGMGAGADMGLEKLGIFVKTVTEGGAAHRDGRIQVNDLLVEVDGTSLVGVTQSFAA
                     SVLRNTKGRVRFMIGRERPGEQSEVAQLIQQTLEQERWQREMMEQRYAQYGEDDEETG
                     EYATDEDEELSPTFPGGEMAIEVFELAENEDALSPVDMEPEKLVHKFKELQIKHAVTE
                     AEIQQLKRKLQSLEQEKGRWRVEKAQLEQSVEENKERMEKLEGYWGEAQSLCQAVDEH
                     LRETQAQYQALERKYSKAKRLIKDYQQKEIEFLKKETAQRRVLEESELARKEEMDKLL
                     DKISELEGNLQTLRNSNST"
     exon            1536..1668
                     /gene="PPP1R9B"
                     /gene_synonym="PPP1R6; PPP1R9; SPINO; Spn"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..1789
                     /gene="PPP1R9B"
                     /gene_synonym="PPP1R6; PPP1R9; SPINO; Spn"
                     /inference="alignment:Splign:2.1.0"
     exon            1790..1894
                     /gene="PPP1R9B"
                     /gene_synonym="PPP1R6; PPP1R9; SPINO; Spn"
                     /inference="alignment:Splign:2.1.0"
     exon            1895..2030
                     /gene="PPP1R9B"
                     /gene_synonym="PPP1R6; PPP1R9; SPINO; Spn"
                     /inference="alignment:Splign:2.1.0"
     exon            2031..2183
                     /gene="PPP1R9B"
                     /gene_synonym="PPP1R6; PPP1R9; SPINO; Spn"
                     /inference="alignment:Splign:2.1.0"
     exon            2184..2237
                     /gene="PPP1R9B"
                     /gene_synonym="PPP1R6; PPP1R9; SPINO; Spn"
                     /inference="alignment:Splign:2.1.0"
     exon            2238..2467
                     /gene="PPP1R9B"
                     /gene_synonym="PPP1R6; PPP1R9; SPINO; Spn"
                     /inference="alignment:Splign:2.1.0"
     exon            2468..2564
                     /gene="PPP1R9B"
                     /gene_synonym="PPP1R6; PPP1R9; SPINO; Spn"
                     /inference="alignment:Splign:2.1.0"
     exon            2565..4212
                     /gene="PPP1R9B"
                     /gene_synonym="PPP1R6; PPP1R9; SPINO; Spn"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4194..4199
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP1R9B"
                     /gene_synonym="PPP1R6; PPP1R9; SPINO; Spn"
                     /note="hexamer: CATAAA"
     polyA_site      4212
                     /gene="PPP1R9B"
                     /gene_synonym="PPP1R6; PPP1R9; SPINO; Spn"
                     /note="major polyA site"
ORIGIN      
        1 aagcgggatt aactgttttt ggcagcttgg ttttgggggg accctaaaaa aggccttcgg
       61 ggtttctttt atcgggggga gggggcggtg gtcccgactt ggccagccgc ctgttgaagc
      121 gggggagcgg ggagcagggg catgcgaacc cggctccccc caccatgatg aagacggagc
      181 cacgggggcc cgggggtccc ctccggagcg cctccccgca ccgcagcgcc tacgaggcgg
      241 gcatccaggc gctgaagccg cccgacgcgc ccgggcccga cgaggcaccc aagggggccc
      301 accacaagaa atatggctcc aacgtccacc gcatcaaaag tatgttcctg cagatgggca
      361 cgacggcggg gccctcgggc gaggcgggcg gcggcgcggg cctggccgag gccccacggg
      421 cgtccgagcg cggcgtgcgc ctgtcgctgc cgcgggccag cagcctgaac gagaacgtgg
      481 accacagcgc cctgctgaag ctgggcacca gcgtgtcgga gcgcgtgagc cgcttcgact
      541 ccaagcccgc gccctccgcg cagcctgcgc cgccgccgca cccgccgtcc cggctgcagg
      601 agacgcggaa gctgttcgaa cggagcgccc cagcggccgc aggcggcgac aaggaggccg
      661 cggcgcggcg gctgctgagg caggagcgcg ccggcctgca ggaccggaag ctggacgtcg
      721 tggtgcgctt caacggcagc accgaggcgc tggacaagct ggacgctgac gccgtgtccc
      781 ccacggtcag ccagctcagc gccgtcttcg agaaggccga ctcgaggacc ggcctccacc
      841 gcgggcccgg gctccccagg gccgcagggg ttccccaggt caactcgaag ctggtcagca
      901 agcggtcccg ggtgttccag cccccgccgc cgccgccgcc cgccccgtcg ggggatgccc
      961 cggccgagaa agagcgatgc cccgcagggc agcagccccc gcagcaccga gtggcccctg
     1021 cccggccgcc ccccaagccc cgggaggtgc gcaagattaa gccggtggag gtggaggaga
     1081 gcggggagtc ggaggccgag tcggcgcccg gggaggtgat ccaggccgag gttacggtcc
     1141 acgcggccct ggagaatggc agcaccgtgg caactgcagc cagccccgcg cccgaggagc
     1201 caaaggccca agcggccccg gagaaggagg cggcggcggt agcgccgcca gagagggggg
     1261 tgggcaatgg ccgggccccg gacgtggccc ctgaggaggt agatgaatcc aagaaggagg
     1321 acttctcgga ggcggacttg gtggacgtga gcgcctacag tgggctcggg gaggactctg
     1381 cgggcagtgc cctggaggag gacgacgaag acgacgagga ggatggggag cccccctacg
     1441 agcccgagtc ggggtgcgtg gagatcccgg ggctgtcgga ggaggaggac ccagccccga
     1501 gccggaagat ccatttcagc acggcgccca tccaagtgtt cagcacttac tccaacgagg
     1561 attacgatcg tcgcaacgag gatgtggatc ccatggcagc ctctgctgag tacgagctgg
     1621 agaagcgtgt ggagaggttg gagctgttcc ctgtggagct ggagaaggac tccgagggcc
     1681 tgggcatcag catcatcggc atgggcgccg gggcagacat gggcctggag aagctgggta
     1741 tcttcgtcaa gaccgtgacg gagggtggtg cggcccatcg ggatggcagg atccaggtga
     1801 atgatctcct ggtggaggtg gatggaacaa gtctggtggg agtgacccag agcttcgcgg
     1861 cgtctgtgct ccggaacacc aagggccgag tgcggtttat gattggccgg gagcggccgg
     1921 gagagcagag cgaagtggcc cagctaattc agcagacttt ggaacaggag cgatggcagc
     1981 gggagatgat ggagcagaga tacgcccagt atggggagga tgacgaggag acgggagagt
     2041 atgccactga cgaggatgag gagctgagcc ccacgttccc gggtggtgag atggccatcg
     2101 aggtgtttga gctagcggag aacgaggatg cactgtcccc tgtggacatg gagcccgaga
     2161 agctggtgca caagttcaag gagctccaga tcaagcatgc ggtcactgag gcagagatcc
     2221 agcagctgaa aagaaagctg cagagcctgg agcaggagaa ggggcgctgg cgggtggaga
     2281 aggcgcagtt ggagcagagt gtggaggaga acaaggagcg catggagaaa ctggaaggct
     2341 actggggtga ggcccagagc ctgtgccagg ctgtggacga gcacctgcgg gagactcagg
     2401 cgcagtacca ggccctggag cgcaagtaca gcaaggccaa gcgcctcatc aaggactacc
     2461 agcagaagga gatcgagttc ctgaaaaagg agactgcaca gcgtcgggtt ctggaggagt
     2521 cggagctggc cagaaaggag gagatggaca agctcctgga caagatctca gaactggaag
     2581 gaaacttgca aacactgagg aattccaatt ctacttaaca ggaatcattc catgactgga
     2641 caataattaa cccccctccc attgtcctcc ctcccctgtc ctcaacaccc cacccctccc
     2701 ccttccagcc tggggacagg tgccccgact ccccccaccc ctccacccca cctcccccag
     2761 cttcagggac cagagggctc atatcacagg cccccttaag atggcctggg cagacagagg
     2821 tggctagaag ggcagcctct ttcttgcccc atggggctga ggcacagagg ccgagggctg
     2881 ccgagggctg gcctggtggt cgacggacac aagcacctgc agatcaaact gccagacttt
     2941 acacactcca gcttttgtct ctggactgga acggggctgg ggctctccca gcatctgcca
     3001 actgggggct gctccctgcc catggggcac ctgggtggcc cctgggcctc ttgacttgag
     3061 attctacctt ctcggccctt ccctctcccc ccatcattgt gccgtctctg ttgcattctg
     3121 accctcccgc ctggggggtg ggggcgttgc ctcaacacct cccccatccc tgttccctgt
     3181 cctgggaggg atagagtcca ccccagagcc cggaggctgg gcctggccct ggccctgcac
     3241 tgatttctca tgtgtccttc gggaaaaggg ggagtgagag tgggaaaaag ggagagttca
     3301 ggacacctgg tccccagctc cccattgcct ggggcagcag ccctcaattt ctccttgtgc
     3361 ctcccctctt ccaggtgcca aatagccata acctcgtctt ggaactgtta tgtggcctct
     3421 ctggggtcca ggtttcctgg tcaagcctgg gaatgccagg gaggaaaggg ggtctggcta
     3481 cagcgaccct ggtcttaggc aaggggaaca tttctccctg gagagtcagg tcctatcctg
     3541 tgctgccctg tctgcccagc ccagggcgat gcctggaagc ctactgacat tgcagggagt
     3601 cagcctcgcc cccaccccct actggttttc caatgttttg actggagggc aaaattttac
     3661 tactactcat ctttttggag accagggctg ccctgctggc agcctgcctt ctaagtgaaa
     3721 tcgactctgt ttccccactt taaccccaaa ttggggcttg gaccaaggga ggtgagacca
     3781 cctcccccag gtcccctccc ctttcaaaat ccatctcatt ttgccacttc atgcccctgc
     3841 cctaactggg tttttgttca ttttttaaaa acagaccacc catcccatcc gttttggctt
     3901 cttgtcccct gtaaatagac catgacttcg atcagtattt cttgtcccca ccccttccta
     3961 tccccagatg tgcccccatc ccctgaagga gctggctgtc tcagtcctgg gctcgcgcac
     4021 ttcaccccgg cagatggagg gggcggaacc gggtgggcgg ggccgcttcg ggctgcttgg
     4081 gccagcggcc acccctgccc agccgtcggg agggccgcat ctctgtatat aatatatata
     4141 tgtatgtatt gttcccggtt ttgtacggac catgccctct gtcaggtcgt ccccataaaa
     4201 gcagccccca ga
//
