LOCUS       NM_001166160           10547 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens protein phosphatase 1 regulatory subunit 9A (PPP1R9A),
            transcript variant 1, mRNA.
ACCESSION   NM_001166160
VERSION     NM_001166160.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10547)
  AUTHORS   Thurner,L., Preuss,K.D., Bewarder,M., Kemele,M., Fadle,N.,
            Regitz,E., Altmeyer,S., Schormann,C., Poeschel,V., Ziepert,M.,
            Walter,S., Roth,P., Weller,M., Szczepanowski,M., Klapper,W.,
            Monoranu,C., Rosenwald,A., Moller,P., Hartmann,S., Hansmann,M.L.,
            Mackensen,A., Schafer,H., Schorb,E., Illerhaus,G., Buslei,R.,
            Bohle,R.M., Stilgenbauer,S., Kim,Y.J. and Pfreundschuh,M.
  TITLE     Hyper-N-glycosylated SAMD14 and neurabin-I as driver autoantigens
            of primary central nervous system lymphoma
  JOURNAL   Blood 132 (26), 2744-2753 (2018)
   PUBMED   30249786
  REMARK    GeneRIF: Hyper-N-glycosylated SAMD14 and neurabin-I as driver
            autoantigens of primary central nervous system lymphoma.
REFERENCE   2  (bases 1 to 10547)
  AUTHORS   Yadav,L., Tamene,F., Goos,H., van Drogen,A., Katainen,R.,
            Aebersold,R., Gstaiger,M. and Varjosalo,M.
  TITLE     Systematic Analysis of Human Protein Phosphatase Interactions and
            Dynamics
  JOURNAL   Cell Syst 4 (4), 430-444 (2017)
   PUBMED   28330616
REFERENCE   3  (bases 1 to 10547)
  AUTHORS   Konopaske,G.T., Subburaju,S., Coyle,J.T. and Benes,F.M.
  TITLE     Altered prefrontal cortical MARCKS and PPP1R9A mRNA expression in
            schizophrenia and bipolar disorder
  JOURNAL   Schizophr Res 164 (1-3), 100-108 (2015)
   PUBMED   25757715
  REMARK    GeneRIF: MARCKS and PPP1R9A might contribute to spine loss in
            schizophrenia and bipolar disorder through their interactions.
REFERENCE   4  (bases 1 to 10547)
  AUTHORS   Finalet Ferreiro,J., Rouhigharabaei,L., Urbankova,H., van der
            Krogt,J.A., Michaux,L., Shetty,S., Krenacs,L., Tousseyn,T., De
            Paepe,P., Uyttebroeck,A., Verhoef,G., Taghon,T., Vandenberghe,P.,
            Cools,J. and Wlodarska,I.
  TITLE     Integrative genomic and transcriptomic analysis identified
            candidate genes implicated in the pathogenesis of hepatosplenic
            T-cell lymphoma
  JOURNAL   PLoS One 9 (7), e102977 (2014)
   PUBMED   25057852
  REMARK    GeneRIF: CHN2, ABCB1 and PPP1R9A expression on chromosome 7 is
            implicated in the pathogenesis of hepatosplenic T-cell lymphoma
            distinguishing it from other malignancies.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10547)
  AUTHORS   Peti,W., Nairn,A.C. and Page,R.
  TITLE     Structural basis for protein phosphatase 1 regulation and
            specificity
  JOURNAL   FEBS J 280 (2), 596-611 (2013)
   PUBMED   22284538
  REMARK    GeneRIF: Studies suggest that any change in substrate specificity
            of the spinophilin : PP1 holoenzyme complex was probably due to
            direct modification of a PP1 substrate binding surface.
            Review article
REFERENCE   6  (bases 1 to 10547)
  AUTHORS   Ragusa,M.J., Dancheck,B., Critton,D.A., Nairn,A.C., Page,R. and
            Peti,W.
  TITLE     Spinophilin directs protein phosphatase 1 specificity by blocking
            substrate binding sites
  JOURNAL   Nat Struct Mol Biol 17 (4), 459-464 (2010)
   PUBMED   20305656
REFERENCE   7  (bases 1 to 10547)
  AUTHORS   Suarez-Gestal,M., Perez-Pampin,E., Calaza,M., Gomez-Reino,J.J. and
            Gonzalez,A.
  TITLE     Lack of replication of genetic predictors for the rheumatoid
            arthritis response to anti-TNF treatments: a prospective case-only
            study
  JOURNAL   Arthritis Res Ther 12 (2), R72 (2010)
   PUBMED   20423481
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
REFERENCE   8  (bases 1 to 10547)
  AUTHORS   Talmud,P.J., Drenos,F., Shah,S., Shah,T., Palmen,J., Verzilli,C.,
            Gaunt,T.R., Pallas,J., Lovering,R., Li,K., Casas,J.P., Sofat,R.,
            Kumari,M., Rodriguez,S., Johnson,T., Newhouse,S.J., Dominiczak,A.,
            Samani,N.J., Caulfield,M., Sever,P., Stanton,A., Shields,D.C.,
            Padmanabhan,S., Melander,O., Hastie,C., Delles,C., Ebrahim,S.,
            Marmot,M.G., Smith,G.D., Lawlor,D.A., Munroe,P.B., Day,I.N.,
            Kivimaki,M., Whittaker,J., Humphries,S.E. and Hingorani,A.D.
  CONSRTM   ASCOT investigators; NORDIL investigators; BRIGHT Consortium
  TITLE     Gene-centric association signals for lipids and apolipoproteins
            identified via the HumanCVD BeadChip
  JOURNAL   Am J Hum Genet 85 (5), 628-642 (2009)
   PUBMED   19913121
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 10547)
  AUTHORS   Wang,X., Zeng,W., Kim,M.S., Allen,P.B., Greengard,P. and Muallem,S.
  TITLE     Spinophilin/neurabin reciprocally regulate signaling intensity by G
            protein-coupled receptors
  JOURNAL   EMBO J 26 (11), 2768-2776 (2007)
   PUBMED   17464283
REFERENCE   10 (bases 1 to 10547)
  AUTHORS   Nakabayashi,K., Makino,S., Minagawa,S., Smith,A.C., Bamforth,J.S.,
            Stanier,P., Preece,M., Parker-Katiraee,L., Paton,T., Oshimura,M.,
            Mill,P., Yoshikawa,Y., Hui,C.C., Monk,D., Moore,G.E. and
            Scherer,S.W.
  TITLE     Genomic imprinting of PPP1R9A encoding neurabin I in skeletal
            muscle and extra-embryonic tissues
  JOURNAL   J Med Genet 41 (8), 601-608 (2004)
   PUBMED   15286155
  REMARK    GeneRIF: PPP1R9A gene is imprinted, with preferential expression
            from the maternal allele in embryonic skeletal muscle and
            extra-embryonic tissues, but biallelic expression in other
            embryonic tissues.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC073886.4, DA497168.1,
            AK096514.1, BC150636.1, BC130449.1, AK000075.1 and AC004022.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001166160.1.
            
            Summary: This gene is imprinted, and located in a cluster of
            imprinted genes on chromosome 7q12. This gene is transcribed in
            both neuronal and multiple embryonic tissues, and it is maternally
            expressed mainly in embryonic skeletal muscle tissues and
            biallelically expressed in other embryonic tissues. The protein
            encoded by this gene includes a PDZ domain and a sterile alpha
            motif (SAM). It is a regulatory subunit of protein phosphatase I,
            and controls actin cytoskeleton reorganization. Alternatively
            spliced transcript variants encoding different isoforms have been
            found for this gene. [provided by RefSeq, Oct 2009].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC150636.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2147975, SAMEA2151741
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene         :: PMID: 15286155
            MANE Ensembl match     :: ENST00000433360.6/ ENSP00000405514.1
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                AC073886.4         59840-59882
            44-81               DA497168.1         1-38
            82-95               AK096514.1         1-14
            96-1460             BC150636.1         1-1365
            1461-2215           BC130449.1         1167-1921
            2216-3440           BC150636.1         2121-3345
            3441-3560           AK000075.1         343-462
            3561-5314           BC150636.1         3466-5219
            5315-10547          AC004022.1         35935-41167
FEATURES             Location/Qualifiers
     source          1..10547
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q21.3"
     gene            1..10547
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /note="protein phosphatase 1 regulatory subunit 9A"
                     /db_xref="GeneID:55607"
                     /db_xref="HGNC:HGNC:14946"
                     /db_xref="MIM:602468"
     exon            1..109
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            110..1720
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     CDS             326..4450
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /note="isoform 1 is encoded by transcript variant 1;
                     neurabin-1; neural tissue-specific F-actin-binding protein
                     I; protein phosphatase 1, regulatory (inhibitor) subunit
                     9A"
                     /codon_start=1
                     /product="neurabin-1 isoform 1"
                     /protein_id="NP_001159632.1"
                     /db_xref="CCDS:CCDS55127.1"
                     /db_xref="GeneID:55607"
                     /db_xref="HGNC:HGNC:14946"
                     /db_xref="MIM:602468"
                     /translation="MLKTESSGERTTLRSASPHRNAYRTEFQALKSTFDKPKSDGEQK
                     TKEGEGSQQSRGRKYGSNVNRIKNLFMQMGMEPNENAAVIAKTRGKGGHSSPQRRMKP
                     KEFLEKTDGSVVKLESSVSERISRFDTMYDGPSYSKFTETRKMFERSVHESGQNNRYS
                     PKKEKAGGSEPQDEWGGSKSNRGSTDSLDSLSSRTEAVSPTVSQLSAVFENTDSPSAI
                     ISEKAENNEYSVTGHYPLNLPSVTVTNLDTFGHLKDSNSWPPSNKRGVDTEDAHKSNA
                     TPVPEVASKSTSLASIPGEEIQQSKEPEDSTSNQQTPDSIDKDGPEEPCAESKAMPKS
                     EIPSPQSQLLEDAEANLVGREAAKQQRKELAGGDFTSPDASASSCGKEVPEDSNNFDG
                     SHVYMHSDYNVYRVRSRYNSDWGETGTEQDEEEDSDENSYYQPDMEYSEIVGLPEEEE
                     IPANRKIKFSSAPIKVFNTYSNEDYDRRNDEVDPVAASAEYELEKRVEKLELFPVELE
                     KDEDGLGISIIGMGVGADAGLEKLGIFVKTVTEGGAAQRDGRIQVNDQIVEVDGISLV
                     GVTQNFAATVLRNTKGNVRFVIGREKPGQVSEVAQLISQTLEQERRQRELLEQHYAQY
                     DADDDENTVAELQGMSGNCNNNNNYFLKTGEYATDEEEDEVGPVLPGSDMAIEVFELP
                     ENEDMFSPSELDTSKLSHKFKELQIKHAVTEAEIQKLKTKLQAAENEKVRWELEKTQL
                     QQNIEENKERMLKLESYWIEAQTLCHTVNEHLKETQSQYQALEKKYNKAKKLIKDFQQ
                     KELDFIKRQEAERKKIEDLEKAHLVEVQGLQVRIRDLEAEVFRLLKQNGTQVNNNNNI
                     FERRTSLGEVSKGDTMENLDGKQTSCQDGLSQDLNEAVPETERLDSKALKTRAQLSVK
                     NRRQRPSRTRLYDSVSSTDGEDSLERKPSNSFYNHMHITKLLPPKGLRTSSPESDSGV
                     PPLTPVDSNVPFSSDHIAEFQEEPLDPEMGPLSSMWGDTSLFSTSKSDHDVEESPCHH
                     QTTNKKILREKDDAKDPKSLRASSSLAVQGGKIKRKFVDLGAPLRRNSSKGKKWKEKE
                     KEASRFSAGSRIFRGRLENWTPKPCSTAQTSTRSPCMPFSWFNDSRKGSYSFRNLPAP
                     TSSLQPSPETLISDKKGSKVENTWITKANKRNPNPSSSSIFGRHSQLMSVVWIQETNN
                     FTFNDDFSPSSTSSADLSGLGAEPKTPGLSQSLALSSDEILDDGQSPKHSQCQNRAVQ
                     EWSVQQVSHWLMSLNLEQYVSEFSAQNITGEQLLQLDGNKLKALGMTASQDRAVVKKK
                     LKEMKMSLEKARKAQEKMEKQREKLRRKEQEQMQRKSKKTEKMTSTTAEGAGEQ"
     exon            1721..1853
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            1854..1974
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            1975..2079
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            2080..2215
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            2216..2281
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            2282..2437
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            2438..2491
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            2492..2721
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            2722..2818
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            2819..2990
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            2991..3148
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            3149..3449
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            3450..3537
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            3538..3621
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            3622..3934
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            3935..4054
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            4055..4237
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     exon            4238..10547
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10530..10535
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /note="hexamer: AATAAA"
     polyA_site      10547
                     /gene="PPP1R9A"
                     /gene_synonym="Neurabin-I; NRB1; NRBI"
                     /note="major polyA site"
ORIGIN      
        1 gcttttcgcc cttccttggc cgccagaggc tccctctctc tccctctccc gggattttgc
       61 tgccgggctc cctctctctg cggccctaga gttaatccca tcagccgaga tgaacctcta
      121 gtcttgcttt gaaaaaatca ttctgtgtaa ctcttgactg tgactaagtt cctaatacac
      181 ctgttgggac gatcactgac accgtatacc atttgagagg tacttttctt gacccaatac
      241 tggtgattag agaagagagg tatcttggtt tttggttttt ttctttgatc attatgaaca
      301 ttggcttttc acccctgaag tgaaaatgtt gaaaactgag tcttcaggtg aacgaaccac
      361 tctcagaagt gcctctcctc acaggaatgc atatcgaact gagtttcagg cactgaaaag
      421 tacctttgac aaacccaagt cagatgggga acaaaaaaca aaagaaggtg agggctccca
      481 gcagagcagg gggaggaaat atggctccaa tgtcaacaga attaaaaacc tatttatgca
      541 gatgggtatg gaacccaacg agaatgctgc agtcattgcc aaaacaaggg ggaaaggtgg
      601 acattcatct cctcagagaa gaatgaagcc caaagaattt ctggaaaaaa cagatggctc
      661 agttgttaag ttggagtctt ctgtttctga acgaattagt agatttgaca ctatgtacga
      721 tggcccttca tattccaagt tcactgagac tcgaaagatg tttgagagaa gtgtgcatga
      781 atcaggacag aacaaccgct attccccaaa gaaagagaaa gctggaggga gtgaacctca
      841 ggatgaatgg ggaggttcca agtccaacag aggcagtact gattccttgg acagccttag
      901 ctcccgaact gaggctgtct ccccaactgt gagtcaactg agtgcagtat ttgagaacac
      961 tgattctccc agtgccatca tttctgagaa ggctgaaaac aatgaatact cagtgactgg
     1021 gcattatccc ttgaatttac catctgttac tgttacaaat cttgacacat ttggtcacct
     1081 gaaggattct aattcctggc ctccttcaaa caagcgaggt gttgatacag aggatgctca
     1141 caagagtaat gcaactccag taccagaagt ggcttctaaa agtacctctc tagcttcgat
     1201 acctggtgaa gagatccagc agagcaagga acccgaggac tccacatcta atcaacagac
     1261 tcccgacagc attgacaaag atggtcctga agaaccttgt gctgaaagta aggcaatgcc
     1321 aaagtccgaa atcccttcac cacaaagcca actgttagaa gatgctgaag ctaatttggt
     1381 tggaagggag gcagcaaagc aacagaggaa agaacttgca ggtggtgatt tcacctctcc
     1441 tgatgcttct gcatccagtt gtggaaaaga agtacctgaa gattcaaata attttgatgg
     1501 ttcccatgtg tacatgcaca gtgactataa tgtgtatagg gtgagatcca ggtataattc
     1561 agactgggga gagacaggca ctgagcagga tgaggaggaa gatagtgatg agaacagtta
     1621 ctatcagcct gatatggagt actcggaaat tgttggattg ccagaagaag aagaaatccc
     1681 agcaaatagg aaaattaagt ttagtagtgc tcctattaag gttttcaaca catactccaa
     1741 tgaagactat gacaggagaa atgacgaagt tgaccctgtg gctgcttcag ctgagtatga
     1801 acttgaaaaa cgtgtagaaa agctggaact tttcccagtg gagctagaga aagatgagga
     1861 tggtcttggt ataagtatta ttggaatggg tgttggagca gatgctggac ttgaaaagct
     1921 gggaatattc gtcaagacag taacagaagg tggtgctgct caacgggatg gcagaataca
     1981 agtcaatgac cagattgtgg aagtggatgg aatcagcttg gtgggtgtga cacagaattt
     2041 tgcagcaaca gttctcagaa acaccaaggg caacgtcaga tttgttattg ggcgggaaaa
     2101 accaggacaa gtgagcgagg ttgcccagtt gataagccag acactggaac aggagaggcg
     2161 ccagagagag ctgctggaac agcactatgc ccagtatgat gccgacgatg acgagaacac
     2221 tgtggctgaa ttgcaaggaa tgtctggcaa ctgcaataac aataacaact attttcttaa
     2281 gacaggagaa tatgccacag atgaagaaga agatgaggta ggacctgtcc ttcctggcag
     2341 cgacatggcc attgaagtct ttgagctgcc tgagaatgag gacatgtttt ccccatcaga
     2401 actggacaca agcaagctca gtcacaagtt caaagagttg caaatcaaac atgcagttac
     2461 agaagcagag attcaaaaat tgaagaccaa gctgcaggca gcagaaaatg agaaagtgag
     2521 gtgggaacta gaaaaaaccc aactccaaca aaacatagaa gagaataagg aaagaatgtt
     2581 gaagttggaa agctactgga ttgaggccca aacattatgc cacacagtga atgagcatct
     2641 caaagagact caaagccagt atcaggcctt ggaaaagaaa tacaacaagg caaagaagtt
     2701 gatcaaggat tttcaacaaa aagagcttga tttcatcaaa agacaggaag cagaaagaaa
     2761 gaaaatagaa gatttggaaa aagctcatct tgtggaagtg caaggcctcc aagtgcggat
     2821 tagagatttg gaagctgagg tattcaggct actgaagcaa aatgggactc aagttaacaa
     2881 taataacaac atctttgaga gaagaacatc tcttggtgaa gtctctaaag gggataccat
     2941 ggagaacttg gatggcaagc agacatcttg ccaagatggc ctaagtcaag acttgaatga
     3001 agcagtccca gagacagagc gcctggattc aaaagcactg aaaactcgag cccagctctc
     3061 tgtgaagaac agacgccaga gaccctctag gacaagactg tatgatagtg ttagttccac
     3121 agatggggag gacagtctag agagaaagcc atcaaacagt ttctataacc acatgcatat
     3181 taccaaatta cttccaccta agggtttgag aacgtcttct ccagaatcag attctggtgt
     3241 tccacccctc accccggtgg atagcaatgt gcccttctcg tctgaccaca tagctgaatt
     3301 tcaagaagaa ccactggacc cagaaatggg gcctctctcc tctatgtggg gagacacttc
     3361 actgttttct acttcaaagt ctgatcatga tgtggaagaa tctccttgcc atcaccaaac
     3421 caccaacaag aaaatattac gagaaaaaga tgatgccaaa gatcccaaat cactaagggc
     3481 atccagttca ttggcggtgc aaggaggaaa aattaagcgg aagtttgtgg atctgggggc
     3541 gcctttgcga aggaattcca gcaagggaaa gaagtggaaa gaaaaagaaa aagaagccag
     3601 taggttttct gcaggtagca ggatcttcag aggcagactg gaaaactgga cacccaagcc
     3661 atgttcaaca gctcagacct ccactcgttc cccttgcatg cctttctcat ggtttaatga
     3721 cagccggaaa ggatcctatt ccttcaggaa cctgcctgcg cctacaagtt cccttcagcc
     3781 ttctcctgag actctaattt cagataaaaa ggggtccaag gtagaaaaca catggattac
     3841 aaaagcaaac aagagaaacc caaatccctc ctcttcttca atctttggaa ggcattctca
     3901 acttatgtct gtagtctgga tccaagaaac caataatttt accttcaatg atgacttcag
     3961 tcccagcagt accagttcag cagacctcag cggcttagga gcagaaccta aaacaccagg
     4021 gctctctcag tccttagcac tgtcatcaga tgagatcctt gatgatggac agtctcccaa
     4081 acacagtcag tgtcagaatc gggccgttca ggaatggagt gtgcagcagg tttctcactg
     4141 gttaatgagc ctaaatctgg agcagtatgt atctgaattc agtgcccaaa acatcactgg
     4201 agaacagctc ctgcagttgg atggaaataa acttaaggct cttggaatga cagcatccca
     4261 ggaccgagca gtggtcaaaa agaaactcaa ggaaatgaag atgtctctag agaaggctcg
     4321 gaaggcccaa gagaaaatgg aaaaacaaag agaaaagcta aggagaaagg agcaagagca
     4381 aatgcagagg aagtccaaaa agacagaaaa gatgacgtca actacagccg agggtgctgg
     4441 tgagcagtaa cacataccct cttacagatg atggagatgc tccaagagaa gtccccacct
     4501 cttcctgccc tgctctcctc cagaggatga aaaagaaact aaatgataag ggtaatgcgg
     4561 ctctaggccg gctgaggaac tgtgtgttga ataactgcat tttctgcaat agaatgcact
     4621 cttaatttta actactaaaa taatcccaag ccacctttgg ttcattaaca aaccagagat
     4681 ttcatttaag tagctgtgtt ttgctcttct ctaacttacc aacatcttgt gttgtgttgg
     4741 gtgtgttttg tcacttggag aactagtgtg accccaccca agagcatgac acaccctggt
     4801 gttgttaatg gagcgccgtg aattttcagt gtgggatcct gaaatggcaa ttgcacatgt
     4861 ctgcatggca gagagcaccc tgccatgaga tctgagacca gtacttaact tctaggactg
     4921 caacacctac tccaatgtgt gggaaagatt tgctcttaat tctcagtctg gacagtgaga
     4981 ccaccccgct gtggaaacat gggtgctctg ctctgtagtt acctactaag ggaaaaggca
     5041 agcaagtcat tctgcttgct aggcatcaaa aagaaagaaa ataaattata gctatttgtt
     5101 aaccactgaa aaaagcaaaa gccttaagaa tgtggatgtg ggtattacct tggggttctg
     5161 agggtaatat ttatcctcta gatacggctg aacaagaaag aaaaaaatca gacattaatg
     5221 cagccatggg atattgggat ctgttgacct tgctactgct gtgtgttcag catttcagca
     5281 acactggcaa acacaacaca gttcctcttc cccctaccta acccaagaaa ttgaacagga
     5341 aattgatttt taaggaaacc accattttca ggtttcctct cctggggaac attctggctg
     5401 gtcttcagcc tgcctatgtc acaatcagga actcatcaca tattttctac tcaagatttc
     5461 ccaggtgggc tcagttagta ttttaacaaa tgtactttta agaaaaatag aaataccagt
     5521 tagaggaaac tgtcagttaa cattttagag agattttgaa tggtttttcc gaaccattcc
     5581 tttccatact tcaaagattc tcaattttac agaaaaagtt tgtttaaggc agtgcatagg
     5641 tatactttag tagtggagga acttatacta attttaagcc agcattaagg atttccatgt
     5701 ttcaccaaac taagtcaagt gcaggagact ggggcagaag aaggagaaaa taaactcgtt
     5761 ttgagaaatt tcatgtgatg agggtttttt gttttttatg ctcatgtatc tggttctctt
     5821 tgctgcgttg actctaaaaa tgaggttatc cactttccaa aggacaagtt tcagaataag
     5881 acttgaggcc acatgaattc ttggacagta ttttacttct tttactttat gggaatctct
     5941 gctctgttcc cagcatctcg tatcttccac tagaagtatt ccatatttag agcctcggca
     6001 aggcttttga gtgtaacaga ttaagggaac tcccttgtag ccccatcttg tattttaata
     6061 acagtgtgat ttttttttat ctgaaaatct gaattcagaa attatctaaa ctggacattt
     6121 ttaattctgt tttaccccca aggcctgttt tcttttcatt gccagagtat tgtcggttat
     6181 gcgtcagctc ttttgtcatt aggtacagac ccttcattca atcctcagaa acgttgacat
     6241 gaagtaacat tgtgttttac agttatcaaa gtcaccatta tgtttttctg tctgtcttca
     6301 ttaggggtat gataatgaaa ctgccgtatt ggatactcta ttaatgtctt gagatgtctg
     6361 gtgctttgtt attttttcac atcatgagaa ataagaagcc actattaatg agtgatacaa
     6421 tttggcaaaa cttttcaaat actaaaacac taaattagta aaaatctggt aatattagtg
     6481 cttgctattg taaatttttg ccatttttgc ttatctttgg gaaaaaagtg acctggatca
     6541 cactggaagt ttcactgtat tcaagagtag aaatagaagg atgattatct taagttatgt
     6601 ttacactttg gtaatatttg aaaatggatg tatatactgg aaatgtctgt aagtgtcttg
     6661 tttttgcacc taatttatga tctattatat tgcattttct taaatatctt agaagtattt
     6721 ttctttataa ctttgtatat tgaatgttta gataaatgtc caactgacaa tgttataaat
     6781 caaattctta ttcctaaaat catttagtat tataagtatt ttgatttttt aagttaaatt
     6841 atagtaattt taggccgaag tgagttattt taacattacc acttaaaacg gatgaaattg
     6901 taaaagtcat ttggtttgat tgccccacaa agcataagat gtaaactgat taagggtatg
     6961 aaagcaaagt gaaagacctc agatcttgtg cacattttgt tttcctatta tttcttttat
     7021 tgactgtagt gcatgatacc taatcaaggc tccaatgtct tgcacaattc ctactttaat
     7081 gctagtggtt ttcagtgtcc ttacaattta acacagaatt gttatttcat caaccttaaa
     7141 aactgcagtt tttattatca gaaaatgaac aaaaagggaa tatctggttg ctatgaactg
     7201 attccctaaa ggagagctca gtatgattct aacaatagcc aaaagtgttt tggggtatag
     7261 gttatgcttt gttgggaggg aatgtgtggt gaggaggcca agctgcagac agaaagcatt
     7321 gtatgagctg tatctgggtc agggtttccc attaaaggat gttagtttta taacagatta
     7381 ctttgtttaa gaccatctcc cttcaagtaa ctcacagttt atgtaatact aatctcattc
     7441 tttttttacc tgaatcagga attttctgca gaatgttaag ttagggaaga aacctcattt
     7501 catccattct aacactcctg atgaagtccc cctttagttt catgacaata aagctgttct
     7561 gtggctatca tcctttagct gggattgaca attgatttca caccctcaac atcacaagct
     7621 ttttttaacc cacagcagtt catgattgca gtatgatagg tactaaagcc aaactcagct
     7681 ttcaactggc acaaaatggg actgtcacct tttgctccat tgttgttgga aacaaagtgg
     7741 caagagggaa aatgaggaac cagatttacc tttgccacta tctgtgaccc tgtaatcatc
     7801 actagcctga aatcttaaaa tatcccagtc ccaagctaca aaaatggtca caggtggttt
     7861 gatctagcac ctcggccgtt agagtttgtg ctgaagacaa atttgtctgg gttcattgtg
     7921 caccagaatt agcatgtgct tgaaaattgc attttataaa agggaaatga aaatttgcat
     7981 ttatatagat actctgattc atgtttaagt gttaaaggtt atgcagacta aacttcaaga
     8041 ggagatccag tgtttaacaa tagtgttcta gaggattcta aaggaagctg ccccagcagc
     8101 cctgagagaa ccatctatac tttggatatg ttgtagcccc atcattacac tgagatattt
     8161 ccatttgagt aactaaaggt actcctgaca gagctggaga tgaggggcat tttggtgggc
     8221 agtggtcact agggttcacg tcacctactt aatgcaaaga ccttagtggt ctcagaatga
     8281 aaaggtaaaa tggaagagaa aagcacagtc ctttcctgtc ttttcaaatt tactttttat
     8341 taaggatgta cctgtgcact attctaaagc taagcattta aggataaagt ccagaaataa
     8401 aatgtccata ttttcttttc ttttcttttt tttttttttt ttctgtcatc tgtcacccag
     8461 actggactgc agtggtgcca tcatgtctta ctgcagcctc aaactcctgg gctcaagtgg
     8521 tcctcctgct tcagcttccc aagtagctgg gactacaggc atgtgccacc acccccagcc
     8581 aaaacatcca tattttctat tagaatggtt tacattatga tgggggatgg ggagctgggg
     8641 gagggctgta cattcccaaa agaaagtagt gttataggaa atccaaatgc gcttcagtga
     8701 ttcactcctg aaaagcagcc tgggtatgtt ggcagatttt gtttgcattt gtcttgcgta
     8761 tttaaagtct caggaaatct agtcttggtc caaaacactg gagaaaaaaa taacaccaga
     8821 caaaacacat ttgagtaact tgaagtagca gcatagaata ggcccaacta agaagttgga
     8881 ggggcaaaga gaaagccgac agaatcagct tctcctggaa agtgaaagca tcaagttcta
     8941 tttatggtag acagagggtc cttatttgct tgtccgtggt gtctatgtgg gcctattgag
     9001 ggaatggtag ttacttgaag gttcacatat gtaagcgtgt ggacccagca cagtctgtga
     9061 cctgattcca gatcctcaag agtggagaag gaaagaaata tgatgatttc ctatgcttga
     9121 ccctaaccca accccaaaac tgctagggat tcaacagtga gtaagatgta tgcaataaga
     9181 gaacagataa cttctcaaca atgtgcatgc cgtttgtgta gatgttcaga actacatatg
     9241 tgtgaatatc actcccacct tacacacgca cacacactgc aatcacatgg cattaaaaaa
     9301 tatattgtgt atggaacttg gcgaggtcag tcaggacctg gagctgtaat catagcctta
     9361 ttgccacttc ttgcgttgtg tatacattga atggctcata gattttaaga gattttttta
     9421 ttgtaagtat ttctactaaa tgcacttatc cttctatatg tttatatatt ttggtaaaat
     9481 tgatttatca gatacttggt attttagtaa gaaaatttcc tcaaatattt ggctaatgct
     9541 ttgatgtcaa gattgcatat tgctgagttg gagctcagaa gaaatgtatg cagctaaaaa
     9601 ttgcactgtg tgttcttgtg aatttgctca caattctaaa atgacataat tggtggctgt
     9661 gatgcttaca gtcactgcct actggctctc ttatgatgaa tgttgccatc atatgatcat
     9721 ttatttactt tttgtgcaag caatggcatt ttggttgttg ccaaaaacaa cacaagtggt
     9781 tgggagtgta tgttgttgga aggtgtacac ttctgtttct ttcagctgta ttatttatac
     9841 acccaggttt tctgttaagg atgtaatcat tcattcaaga aagaacaatt ggcaagaaat
     9901 aattaaatta ccttaaaaag aattaagagg cactccagcc agataacacc tcccaacaac
     9961 atgaaattta gttagtgctt agtgaaattc cttaaatata tatgtatatg ttttcttcaa
    10021 cattatttaa tataactact gcttttatat tttttaatgg tgttgcaacc acttgtacac
    10081 ctgctacacc ttactgacaa aactgggcag ctgaaaataa aagagaataa attctattcc
    10141 actgtaagaa gttgatttct tttcagcttt ctttgtatat cagtaaagaa cacgaattgt
    10201 ttgaaaataa ttttaagtct gataagtatg gatagcatgt tacctaagct tttaatttgt
    10261 acattttgat gtctgatcat ttgcatggaa gagacaatag tgccacgtct gaattgttct
    10321 cttgtgcttg gttaatatgt acttctctag attgttcaaa aagtttcaaa tgacattcct
    10381 tactctttgg ttttcagagg cattcaagca aaatatcaca gtggtctttt gttttctgac
    10441 caatctaaca atacctgtga ttaaatttaa tttgttagtg taaataaatt tcttgccaat
    10501 gagtattatt gttgttagac aacgaatgca ataaaaagaa atactga
//
