LOCUS       NM_014110               2340 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens protein phosphatase 1 regulatory subunit 8 (PPP1R8),
            transcript variant 1, mRNA.
ACCESSION   NM_014110
VERSION     NM_014110.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Hanaki,S., Habara,M., Masaki,T., Maeda,K., Sato,Y., Nakanishi,M.
            and Shimada,M.
  TITLE     PP1 regulatory subunit NIPP1 regulates transcription of E2F1 target
            genes following DNA damage
  JOURNAL   Cancer Sci 112 (7), 2739-2752 (2021)
   PUBMED   33939241
  REMARK    GeneRIF: PP1 regulatory subunit NIPP1 regulates transcription of
            E2F1 target genes following DNA damage.
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2340)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2340)
  AUTHORS   Lee,J.H., Han,J.H., Kim,H., Park,S.M., Joe,E.H. and Jou,I.
  TITLE     Parkinson's disease-associated LRRK2-G2019S mutant acts through
            regulation of SERCA activity to control ER stress in astrocytes
  JOURNAL   Acta Neuropathol Commun 7 (1), 68 (2019)
   PUBMED   31046837
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2340)
  AUTHORS   Winkler,C., Rouget,R., Wu,D., Beullens,M., Van Eynde,A. and
            Bollen,M.
  TITLE     Overexpression of PP1-NIPP1 limits the capacity of cells to repair
            DNA double-strand breaks
  JOURNAL   J Cell Sci 131 (13) (2018)
   PUBMED   29898919
  REMARK    GeneRIF: PP1-NIPP1 expression resulted in the build up of RNA-DNA
            hybrids (R-loops), enhanced chromatin compaction and a diminished
            repair of DNA double-strand breaks.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2340)
  AUTHORS   Vulsteke,V., Beullens,M., Waelkens,E., Stalmans,W. and Bollen,M.
  TITLE     Properties and phosphorylation sites of baculovirus-expressed
            nuclear inhibitor of protein phosphatase-1 (NIPP-1)
  JOURNAL   J Biol Chem 272 (52), 32972-32978 (1997)
   PUBMED   9407077
REFERENCE   7  (bases 1 to 2340)
  AUTHORS   Claverie-Martin,F., Wang,M. and Cohen,S.N.
  TITLE     ARD-1 cDNA from human cells encodes a site-specific single-strand
            endoribonuclease that functionally resembles Escherichia coli RNase
            E
  JOURNAL   J Biol Chem 272 (21), 13823-13828 (1997)
   PUBMED   9153239
REFERENCE   8  (bases 1 to 2340)
  AUTHORS   Van Eynde,A., Wera,S., Beullens,M., Torrekens,S., Van Leuven,F.,
            Stalmans,W. and Bollen,M.
  TITLE     Molecular cloning of NIPP-1, a nuclear inhibitor of protein
            phosphatase-1, reveals homology with polypeptides involved in RNA
            processing
  JOURNAL   J Biol Chem 270 (47), 28068-28074 (1995)
   PUBMED   7499293
REFERENCE   9  (bases 1 to 2340)
  AUTHORS   Wang,M. and Cohen,S.N.
  TITLE     ard-1: a human gene that reverses the effects of
            temperature-sensitive and deletion mutations in the Escherichia
            coli rne gene and encodes an activity producing RNase E-like
            cleavages
  JOURNAL   Proc Natl Acad Sci U S A 91 (22), 10591-10595 (1994)
   PUBMED   7524097
REFERENCE   10 (bases 1 to 2340)
  AUTHORS   Mishima,K., Tsuchiya,M., Nightingale,M.S., Moss,J. and Vaughan,M.
  TITLE     ARD 1, a 64-kDa guanine nucleotide-binding protein with a
            carboxyl-terminal ADP-ribosylation factor domain
  JOURNAL   J Biol Chem 268 (12), 8801-8807 (1993)
   PUBMED   8473324
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA167043.1, AK292077.1,
            AF061959.1 and AL109927.61.
            
            On Nov 22, 2018 this sequence version replaced NM_014110.4.
            
            Summary: This gene, through alternative splicing, encodes three
            different isoforms. Two of the protein isoforms encoded by this
            gene are specific inhibitors of type 1 serine/threonine protein
            phosphatases and can bind but not cleave RNA. The third protein
            isoform lacks the phosphatase inhibitory function but is a
            single-strand endoribonuclease comparable to RNase E of E. coli.
            This isoform requires magnesium for its function and cleaves
            specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (1) is the shortest transcript but
            encodes the predominant and longest isoform (alpha). Isoform alpha
            contains protein phosphatase inhibitory actvity.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.245161.1,
                                           SRR1163655.145631.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000311772.10/ ENSP00000311677.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                DA167043.1         42-73
            33-1129             AK292077.1         2-1098
            1130-2336           AF061959.1         1254-2460
            2337-2340           AL109927.61        8699-8702
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p35.3"
     gene            1..2340
                     /gene="PPP1R8"
                     /gene_synonym="ARD-1; ARD1; NIPP-1; NIPP1; PRO2047"
                     /note="protein phosphatase 1 regulatory subunit 8"
                     /db_xref="GeneID:5511"
                     /db_xref="HGNC:HGNC:9296"
                     /db_xref="MIM:602636"
     exon            1..110
                     /gene="PPP1R8"
                     /gene_synonym="ARD-1; ARD1; NIPP-1; NIPP1; PRO2047"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..1110
                     /gene="PPP1R8"
                     /gene_synonym="ARD-1; ARD1; NIPP-1; NIPP1; PRO2047"
                     /note="isoform alpha is encoded by transcript variant 1;
                     activator of RNA decay; nuclear subunit of PP-1; nuclear
                     inhibitor of protein phosphatase 1; RNase E; protein
                     phosphatase 1, regulatory (inhibitor) subunit 8; nuclear
                     inhibitor of protein phosphatase-1 alpha; nuclear
                     inhibitor of protein phosphatase-1 beta"
                     /codon_start=1
                     /product="nuclear inhibitor of protein phosphatase 1
                     isoform alpha"
                     /protein_id="NP_054829.2"
                     /db_xref="CCDS:CCDS311.1"
                     /db_xref="GeneID:5511"
                     /db_xref="HGNC:HGNC:9296"
                     /db_xref="MIM:602636"
                     /translation="MAAAANSGSSLPLFDCPTWAGKPPPGLHLDVVKGDKLIEKLIID
                     EKKYYLFGRNPDLCDFTIDHQSCSRVHAALVYHKHLKRVFLIDLNSTHGTFLGHIRLE
                     PHKPQQIPIDSTVSFGASTRAYTLREKPQTLPSAVKGDEKMGGEDDELKGLLGLPEEE
                     TELDNLTEFNTAHNKRISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEIINPEDVDPS
                     VGRFRNMVQTAVVPVKKKRVEGPGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSEAGS
                     QPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKK
                     YAKEAWPGKKPTPSLLI"
     exon            111..171
                     /gene="PPP1R8"
                     /gene_synonym="ARD-1; ARD1; NIPP-1; NIPP1; PRO2047"
                     /inference="alignment:Splign:2.1.0"
     exon            172..325
                     /gene="PPP1R8"
                     /gene_synonym="ARD-1; ARD1; NIPP-1; NIPP1; PRO2047"
                     /inference="alignment:Splign:2.1.0"
     exon            326..546
                     /gene="PPP1R8"
                     /gene_synonym="ARD-1; ARD1; NIPP-1; NIPP1; PRO2047"
                     /inference="alignment:Splign:2.1.0"
     exon            547..691
                     /gene="PPP1R8"
                     /gene_synonym="ARD-1; ARD1; NIPP-1; NIPP1; PRO2047"
                     /inference="alignment:Splign:2.1.0"
     exon            692..756
                     /gene="PPP1R8"
                     /gene_synonym="ARD-1; ARD1; NIPP-1; NIPP1; PRO2047"
                     /inference="alignment:Splign:2.1.0"
     exon            757..2340
                     /gene="PPP1R8"
                     /gene_synonym="ARD-1; ARD1; NIPP-1; NIPP1; PRO2047"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2173..2178
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP1R8"
                     /gene_synonym="ARD-1; ARD1; NIPP-1; NIPP1; PRO2047"
                     /note="hexamer: ATTAAA"
     polyA_site      2192
                     /gene="PPP1R8"
                     /gene_synonym="ARD-1; ARD1; NIPP-1; NIPP1; PRO2047"
                     /note="major polyA site"
     regulatory      2312..2317
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP1R8"
                     /gene_synonym="ARD-1; ARD1; NIPP-1; NIPP1; PRO2047"
                     /note="hexamer: AATAAA"
     polyA_site      2340
                     /gene="PPP1R8"
                     /gene_synonym="ARD-1; ARD1; NIPP-1; NIPP1; PRO2047"
ORIGIN      
        1 cttccagttt cccggcgtgc ttagggcgcg ccaaatggga gggggagacg caagatggcg
       61 gcagccgcga actccggctc tagcctcccg ctgttcgact gcccaacctg ggcaggtaag
      121 ccccctcccg gtttacatct ggatgtagtc aaaggagaca aactaattga gaaactgatt
      181 attgatgaga agaagtatta cttatttggg agaaaccctg atttgtgtga ctttaccatt
      241 gaccaccagt cttgctctcg ggtccatgct gcacttgtct accacaagca tctgaagaga
      301 gttttcctga tagatctcaa cagtacacac ggcactttct tgggtcacat tcggttggaa
      361 cctcacaagc ctcagcaaat tcccatcgat tccacggtct catttggcgc atccacaagg
      421 gcatacactc tgcgcgagaa gcctcagaca ttgccatcgg ctgtgaaagg agatgagaag
      481 atgggtggag aggatgatga actcaagggc ttactggggc ttccagagga ggaaactgag
      541 cttgataacc tgacagagtt caacactgcc cacaacaagc ggatttctac ccttaccatt
      601 gaggagggaa atctggacat tcaaagacca aagaggaaga ggaagaactc acgggtgaca
      661 ttcagtgagg atgatgagat catcaaccca gaggatgtgg atccctcagt tggtcgattc
      721 aggaacatgg tgcaaactgc agtggtccca gtcaagaaga agcgtgtgga gggccctggc
      781 tccctgggcc tggaggaatc agggagcagg cgcatgcaga actttgcctt cagcggagga
      841 ctctacgggg gcctgccccc cacacacagt gaagcaggct cccagccaca tggcatccat
      901 gggacagcac tcatcggtgg cttgcccatg ccatacccaa accttgcccc tgatgtggac
      961 ttgactcctg ttgtgccgtc agcagtgaac atgaaccctg caccaaaccc tgcagtctat
     1021 aaccctgaag ctgtaaatga acccaagaag aagaaatatg caaaagaggc ttggccaggc
     1081 aagaagccca caccttcctt gctgatttga tatttttggt catggagaag ggtgggattg
     1141 ggtgggaatg gggtggaagg gtgatgggga gctaatgaac tagggagaaa aactttccat
     1201 gtgtgcggta tcgtctttca gaatgtctcc tggcatccta accatgtaat atgacaattg
     1261 ggggtggggt tgaaatagcc cataaagacc tgtcttcaca acacttgcat tgtagagaaa
     1321 ggcttcttat atccttttca atagactgcc ctggctcttt cctaggcctt ccactacctc
     1381 ctttctttct cccactttct aggatcattt ttatgtaaag tcacatatcc caggccctca
     1441 ggttgaatcc agagctgtag aggttacagt agcatcacca gccttggggg tccagagcct
     1501 aatttatatt cactatcctt ccaagtcccg ggtagcagaa gggttgccat agatctcagt
     1561 ttgatcaaaa agaaggctta gaattctgca gttaagctga ggtttaaact aaaaaatgtt
     1621 tccttgggtc agtggttttg aggtccagta gctaggcttt tctcttttgt ccttcctgtt
     1681 ggaatgaaaa catttcgatt ttccttcatc tgtgactggt gccatagaca caggtttata
     1741 gttttaactt acagtattgt ttgaaattta cctgtttttc ttgtcaaacc tgagcactcc
     1801 tcctgctgaa gtttcttatt taattccaga gtactgtcct ctactctaag gcattacttt
     1861 taagtgtatt atgaaggcag ttttcaaagg atatgaccag ttggggtaat tcaaattaaa
     1921 aaggaaaaga tttgtttgga agtaactggt gtctctaaga ggaattttta gatgtcagtt
     1981 tggaggctct ttcccccctc aattgagagc tcttgttatt cagagctcca agactagacc
     2041 tggctaacaa acataggaga caaagttagg aaacattgat acaagctttg tacagagatt
     2101 tgtacatttg tgtaataggc cttttcatgc tttatgtgta gctttttacc tgtaaccttt
     2161 attacattgt aaattaaacg taacttttgt catttgggtg caggctgtga atttgtctct
     2221 cagtcactga ttgccactgc catctggaaa tgtttgctaa aggcacagtc actgggcttg
     2281 ggaggcaatg ctccatcccc attatattac aaataaagat gccctaaatg agtgtggaaa
//
