LOCUS       NM_145030                963 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens protein phosphatase 1 regulatory subunit 35 (PPP1R35),
            transcript variant 1, mRNA.
ACCESSION   NM_145030
VERSION     NM_145030.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Fong,C.S., Ozaki,K. and Tsou,M.B.
  TITLE     PPP1R35 ensures centriole homeostasis by promoting
            centriole-to-centrosome conversion
  JOURNAL   Mol Biol Cell 29 (23), 2801-2808 (2018)
   PUBMED   30230954
  REMARK    GeneRIF: Authors found that PPP1R35 is enriched at newborn daughter
            centrioles in S or G2 phase. In the absence of PPP1R35, centriole
            assembly initiates normally in S phase, but none of the nascent
            centrioles can form active centrosomes or recruit CEP295, an
            essential factor for CCC.
REFERENCE   2  (bases 1 to 963)
  AUTHORS   Sydor,A.M., Coyaud,E., Rovelli,C., Laurent,E., Liu,H., Raught,B.
            and Mennella,V.
  TITLE     PPP1R35 is a novel centrosomal protein that regulates centriole
            length in concert with the microcephaly protein RTTN
  JOURNAL   Elife 7, e37846 (2018)
   PUBMED   30168418
  REMARK    GeneRIF: Data show that PPP1R35 acts downstream of, and forms a
            complex with, RTTN, a microcephaly protein required for distal
            centriole elongation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 963)
  AUTHORS   Hendrickx,A., Beullens,M., Ceulemans,H., Den Abt,T., Van Eynde,A.,
            Nicolaescu,E., Lesage,B. and Bollen,M.
  TITLE     Docking motif-guided mapping of the interactome of protein
            phosphatase-1
  JOURNAL   Chem Biol 16 (4), 365-371 (2009)
   PUBMED   19389623
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC092849.4, BI752874.1, BC026269.1 and AI433518.1.
            
            On Nov 22, 2018 this sequence version replaced NM_145030.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BM980874.1, CB851117.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142348
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000292330.3/ ENSP00000292330.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AC092849.4         54983-55006
            25-26               BI752874.1         1-2
            27-956              BC026269.1         1-930
            957-963             AI433518.1         15-21               c
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q22.1"
     gene            1..963
                     /gene="PPP1R35"
                     /gene_synonym="C7orf47"
                     /note="protein phosphatase 1 regulatory subunit 35"
                     /db_xref="GeneID:221908"
                     /db_xref="HGNC:HGNC:28320"
                     /db_xref="MIM:618937"
     exon            1..357
                     /gene="PPP1R35"
                     /gene_synonym="C7orf47"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..885
                     /gene="PPP1R35"
                     /gene_synonym="C7orf47"
                     /note="isoform 1 is encoded by transcript variant 1;
                     UPF0683 protein C7orf47"
                     /codon_start=1
                     /product="protein phosphatase 1 regulatory subunit 35
                     isoform 1"
                     /protein_id="NP_659467.1"
                     /db_xref="CCDS:CCDS5694.1"
                     /db_xref="GeneID:221908"
                     /db_xref="HGNC:HGNC:28320"
                     /db_xref="MIM:618937"
                     /translation="MMMGCGESELKSADGEEAAAVPGPPPEPQVPQLRAPVPEPGLDL
                     SLSPRPDSPQPRHGSPGRRKGRAERRGAARQRRQVRFRLTPPSPVRSEPQPAVPQELE
                     MPVLKSSLALGLELRAAAGSHFDAAKAVEEQLRKSFQIRCGLEESVSEGLNVPRSKRL
                     FRDLVSLQVPEEQVLNAALREKLALLPPQARAPHPKEPPGPGPDMTILCDPETLFYES
                     PHLTLDGLPPLRLQLRPRPSEDTFLMHRTLRRWEA"
     exon            358..574
                     /gene="PPP1R35"
                     /gene_synonym="C7orf47"
                     /inference="alignment:Splign:2.1.0"
     exon            575..711
                     /gene="PPP1R35"
                     /gene_synonym="C7orf47"
                     /inference="alignment:Splign:2.1.0"
     exon            712..963
                     /gene="PPP1R35"
                     /gene_synonym="C7orf47"
                     /inference="alignment:Splign:2.1.0"
     regulatory      941..946
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP1R35"
                     /gene_synonym="C7orf47"
                     /note="hexamer: AATAAA"
     polyA_site      963
                     /gene="PPP1R35"
                     /gene_synonym="C7orf47"
                     /note="major polyA site"
ORIGIN      
        1 aacggtttcc ggtagcggcg tctaggaggg gcgggggaaa ggaggcggca gccaggctgt
       61 gtcccctgac cgttggagcg tctgcgaccc ccgcatcccc gcaccctcaa ggcacctcca
      121 aagatgatga tgggttgtgg ggagtcagag ctgaagtcgg cggacgggga agaagccgcg
      181 gcggtcccgg ggccaccccc ggagccccaa gtcccgcaac tccgagcccc agtgcccgag
      241 cccggcctgg acttgagcct gagcccgcgg cccgacagcc ctcagccgcg gcacggcagc
      301 cccgggcggc ggaaggggcg ggcggagcgg cggggcgcgg ctcggcagcg gcggcaggtc
      361 cgcttccgcc tgacgccgcc ctccccggtg cggtccgagc cgcagcctgc ggtgccgcag
      421 gagctggaga tgcccgtgct gaagagcagc ctggccttgg gcctggagct gcgggccgca
      481 gccgggagcc actttgatgc tgcgaaggcc gtggaggaac agctgagaaa gtcgttccag
      541 atccgctgcg gcctggagga gagcgtgtcc gaggggctga acgtgccgcg ctccaagcgg
      601 ctcttccggg acctggtgag cctgcaggtg ccggaggaac aggttctgaa tgccgcgctc
      661 agggagaaat tggctctcct gccgccacag gctcgagccc cgcacccaaa ggagccacct
      721 gggcctgggc cagacatgac catcttgtgt gacccagaaa cgctatttta tgaatctcca
      781 cacctgaccc tggacggtct gccccctctc cgacttcaac tccggccccg cccttcagag
      841 gacaccttcc tcatgcaccg gacactgagg cgatgggaag cgtagacccc aaagatccct
      901 ggagggctag ttcgtatttt tgtgttaaac tatttgttag aataaagtaa ttttgctaat
      961 aaa
//
