LOCUS       NM_006241               3386 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens protein phosphatase 1 regulatory inhibitor subunit 2
            (PPP1R2), transcript variant 2, mRNA.
ACCESSION   NM_006241
VERSION     NM_006241.8
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Salokas,K., Liu,X., Ohman,T., Chowdhury,I., Gawriyski,L.,
            Keskitalo,S. and Varjosalo,M.
  TITLE     Physical and functional interactome atlas of human receptor
            tyrosine kinases
  JOURNAL   EMBO Rep 23 (6), e54041 (2022)
   PUBMED   35384245
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3386)
  AUTHORS   Bresch,A.M., Yerich,N., Wang,R. and Sperry,A.O.
  TITLE     The PP1 regulator PPP1R2 coordinately regulates AURKA and PP1 to
            control centrosome phosphorylation and maintain central spindle
            architecture
  JOURNAL   BMC Mol Cell Biol 21 (1), 84 (2020)
   PUBMED   33238888
  REMARK    GeneRIF: The PP1 regulator PPP1R2 coordinately regulates AURKA and
            PP1 to control centrosome phosphorylation and maintain central
            spindle architecture.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3386)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 3386)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 3386)
  AUTHORS   Kakinoki,Y., Somers,J. and Brautigan,D.L.
  TITLE     Multisite phosphorylation and the nuclear localization of
            phosphatase inhibitor 2-green fluorescent protein fusion protein
            during S phase of the cell growth cycle
  JOURNAL   J Biol Chem 272 (51), 32308-32314 (1997)
   PUBMED   9405437
REFERENCE   7  (bases 1 to 3386)
  AUTHORS   Permana,P.A. and Mott,D.M.
  TITLE     Genetic analysis of human type 1 protein phosphatase inhibitor 2 in
            insulin-resistant Pima Indians
  JOURNAL   Genomics 41 (1), 110-114 (1997)
   PUBMED   9126490
REFERENCE   8  (bases 1 to 3386)
  AUTHORS   Sanseau,P., Jackson,A., Alderton,R.P., Beck,S., Senger,G.,
            Sheer,D., Kelly,A. and Trowsdale,J.
  TITLE     Cloning and characterization of human phosphatase inhibitor-2
            (IPP-2) sequences
  JOURNAL   Mamm Genome 5 (8), 490-496 (1994)
   PUBMED   7949733
REFERENCE   9  (bases 1 to 3386)
  AUTHORS   Helps,N.R., Street,A.J., Elledge,S.J. and Cohen,P.T.
  TITLE     Cloning of the complete coding region for human protein phosphatase
            inhibitor 2 using the two hybrid system and expression of inhibitor
            2 in E. coli
  JOURNAL   FEBS Lett 340 (1-2), 93-98 (1994)
   PUBMED   8119416
REFERENCE   10 (bases 1 to 3386)
  AUTHORS   Park,I.K., Roach,P., Bondor,J., Fox,S.P. and DePaoli-Roach,A.A.
  TITLE     Molecular mechanism of the synergistic phosphorylation of
            phosphatase inhibitor-2. Cloning, expression, and site-directed
            mutagenesis of inhibitor-2
  JOURNAL   J Biol Chem 269 (2), 944-954 (1994)
   PUBMED   8288648
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AA259068.1, AC069213.22 and
            BC007655.2.
            
            On Nov 23, 2018 this sequence version replaced NM_006241.7.
            
            Summary: Protein phosphatase-1 (PP1) is one of the main eukaryotic
            serine/threonine phosphatases. The protein encoded by this gene
            binds to the catalytic subunit of PP1, strongly inhibiting its
            activity. Ten related pseudogenes have been found throughout the
            human genome. Several splice variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, Oct 2015].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site, compared to variant 1. The encoded isoform (2) is
            shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.247833.1,
                                           SRR1803615.254934.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000618156.5/ ENSP00000484580.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 AA259068.1         138-144             c
            8-75                AC069213.22        85207-85274         c
            76-2084             BC007655.2         1-2009
            2085-2085           AC069213.22        57685-57685         c
            2086-2803           BC007655.2         2011-2728
            2804-3386           BC007655.2         2730-3312
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q29"
     gene            1..3386
                     /gene="PPP1R2"
                     /gene_synonym="IPP-2; IPP2; PPP1R2A"
                     /note="protein phosphatase 1 regulatory inhibitor subunit
                     2"
                     /db_xref="GeneID:5504"
                     /db_xref="HGNC:HGNC:9288"
                     /db_xref="MIM:601792"
     exon            1..422
                     /gene="PPP1R2"
                     /gene_synonym="IPP-2; IPP2; PPP1R2A"
                     /inference="alignment:Splign:2.1.0"
     CDS             301..918
                     /gene="PPP1R2"
                     /gene_synonym="IPP-2; IPP2; PPP1R2A"
                     /EC_number="3.1.3.16"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein phosphatase inhibitor 2; phosphoprotein
                     phosphatase"
                     /codon_start=1
                     /product="protein phosphatase inhibitor 2 isoform 2"
                     /protein_id="NP_006232.1"
                     /db_xref="CCDS:CCDS3309.1"
                     /db_xref="GeneID:5504"
                     /db_xref="HGNC:HGNC:9288"
                     /db_xref="MIM:601792"
                     /translation="MAASTASHRPIKGILKNKTSTTSSMVASAEQPRGNVDEELSKKS
                     QKWDEMNILATYHPADKDYGLMKIDEPSTPYHSMMGDDEDACSDTEATEAMAPDILAR
                     KLAAAEGLEPKYRIQEQESSGEEDSDLSPEEREKKRQFEMKRKLHYNEGLNIKLARQL
                     ISKDLHDDDEDEEMLETADGESMNTEESNQGSTPSDQQQNKLRSS"
     exon            423..530
                     /gene="PPP1R2"
                     /gene_synonym="IPP-2; IPP2; PPP1R2A"
                     /inference="alignment:Splign:2.1.0"
     exon            531..608
                     /gene="PPP1R2"
                     /gene_synonym="IPP-2; IPP2; PPP1R2A"
                     /inference="alignment:Splign:2.1.0"
     exon            609..703
                     /gene="PPP1R2"
                     /gene_synonym="IPP-2; IPP2; PPP1R2A"
                     /inference="alignment:Splign:2.1.0"
     exon            704..871
                     /gene="PPP1R2"
                     /gene_synonym="IPP-2; IPP2; PPP1R2A"
                     /inference="alignment:Splign:2.1.0"
     exon            872..3386
                     /gene="PPP1R2"
                     /gene_synonym="IPP-2; IPP2; PPP1R2A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1542..1547
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP1R2"
                     /gene_synonym="IPP-2; IPP2; PPP1R2A"
                     /note="hexamer: AATAAA"
     polyA_site      1566
                     /gene="PPP1R2"
                     /gene_synonym="IPP-2; IPP2; PPP1R2A"
                     /note="major polyA site"
     regulatory      3367..3372
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP1R2"
                     /gene_synonym="IPP-2; IPP2; PPP1R2A"
                     /note="hexamer: AATAAA"
     polyA_site      3386
                     /gene="PPP1R2"
                     /gene_synonym="IPP-2; IPP2; PPP1R2A"
                     /note="major polyA site"
ORIGIN      
        1 gcttcggcgt cgcgtcgctc ctgcgctgga gctctaggcc ggcgtctctc cgcgagccgc
       61 gggtcaagtg ccagtggcta agggtgcgcg agtagccgcc acccgttcgg cgttggctct
      121 ggcgtcgggg tcgttgtgtc gtgacaaccg ctgcggtagc cgtttccgag gcagcagttg
      181 cggccgcttt agccctgagc gggatctgcg gctgcctgcg agtctctgct gtgccgaccc
      241 ttctcttcgc ggaccccacg ccaagcagcg accctgagcc gacagccgga gcgcccggca
      301 atggcggcct cgacggcctc gcaccggccc atcaagggga tcttgaagaa caagacctct
      361 acgacttcct ctatggtggc gtcggccgaa cagccccgcg ggaatgtcga cgaggagctg
      421 agcaaaaaat cccagaagtg ggatgaaatg aacatcttgg cgacgtatca tccagcagac
      481 aaagactatg gtttaatgaa aatagatgaa ccaagcactc cttaccatag tatgatgggg
      541 gatgatgaag atgcctgtag tgacaccgag gccactgaag ccatggcgcc agacatctta
      601 gccaggaaat tagctgcagc tgaaggcttg gagccaaagt atcggattca ggaacaagaa
      661 agcagtggag aggaggatag tgacctctca cctgaagaac gagaaaaaaa gcgacaattt
      721 gaaatgaaaa ggaagcttca ctacaatgaa ggactcaata tcaaactagc cagacaatta
      781 atttcaaaag acctacatga tgatgatgaa gatgaagaaa tgttagagac tgcagatgga
      841 gaaagcatga atacggaaga atcaaatcaa ggatctactc caagtgacca acagcaaaac
      901 aaattacgaa gttcatagac gagatttgtt caacactgca attgtttgtt agatgtaaac
      961 cctgtgacta tagtacgttg cttcttgttc ttcacaattc atgacttaag taccaaaatg
     1021 cataccagtt attatatatt gccaagaatt aaatgataaa cttagagact gattagactg
     1081 aaaatgccta atcgatatat atattcttgt gcctagtact ttaccacaaa tacagtgtaa
     1141 tatcatcagt ccaaaactgc attacttttg taaaaacact ggttaatttg tataagatat
     1201 tatagagctt tttatgcttt agaagttaaa caatatcttt gggggggaac taatttattt
     1261 tcatcacttg aaatgtggta gctcttacaa agtttattga tttgattttt ttaaaaatca
     1321 aaagccaatt gaacaacagg atatatagac tgataaatat ttaggctgaa tagtatttta
     1381 acacttgtct tcaacttgat ttgtctgttt aattgaaaag aattataaga gttactgttg
     1441 cattttctga cctactattt ttaaaattcc tgttgagttt ctttgtgttt acaaggaaag
     1501 gactgaactt tttctcatca aaactagctt ttttccccac aaataaatta tcaggttaaa
     1561 ctttcaccaa tgtctgctct gttttttgtt tttggggggt ttttgtttgt ttttgttttt
     1621 taatgttttt ggtacactgg gcagacttca gagcagtttt ttaaaaaaat aaatattcta
     1681 atgtagctat ctcgccattc cctttaaata cctgtcttaa cctcctgctt ttctttccta
     1741 ctcctttcca cacatacgca cacaatcttc taccttttaa aggatcatta agactgtcac
     1801 cacattagga gctctttctc tcactcttct gtcatttgct gctatgttga aattcttatt
     1861 ttgaccatca atgcctatga attcttctaa tacgtgaaga aaatagtttg agtagcagca
     1921 gtgctatagg tgggaaatac agtttagctg ctgaatttct atacctctct gatttacaga
     1981 ttgctaatta aatggctatt attagtttgg attaattaga cttaagaaaa caactaactg
     2041 agggtttttt ttgtttgttt tttgagggtt ttctttgcat gagaattgta tgtaaccagt
     2101 gatatgatta ttcctgaatg tacagacaga agtaagcctg gacattgtta aatagtccct
     2161 gctttaaggg actacgataa tgtgtactat gacaaacgtg ctttattctt ctaacgcagt
     2221 aagaattagg tggaattttt tccttcaacc aagtgcagga aagccctgtg tgtcttggtt
     2281 tagttatggt ttcatttcta gccatacaat tgatgaattg tgtacaactt ttgttagtac
     2341 caaaataatc tgttatatga acagacttct aaaataacgt ctgtatattt tatatataga
     2401 tacatatatg aaagaaggct tttattgaac agcttatctt ccacttgcag gtttatggaa
     2461 acagcagtat ttgaaaataa ataaaaagtg ggagaattcc ttgctgttag aagaatgtgg
     2521 ccattatttt gattttttga atgagatata taatcaaagt actgctgaac tgtgagtgca
     2581 gtattctaaa catttcagct aggaatacca ctgatttaga aacaaaactg tttgtctctg
     2641 gtttctgaat ttaaaatgtt gggattacct gtttaaatct gtcttggggg atgtagagat
     2701 taagtctgta catgtgcgtg cacatatatt catgcaccct ctgattttgg ttttcttgtt
     2761 tctgagttct tagaaagtac ccacatactc tttttttttt tttgtagaag tagctgtcat
     2821 agagtgaaga aaaggataag actttaaaca gttgattctt tttgtgtttt ctacaaacta
     2881 ttttttgaaa tttaaatcac aaactcattt tctggttttt agaaagtaga tgatgatttc
     2941 agaggagtaa ggcatgccaa gcagcatgct cagtggggtt ttaggctgtc acatgcagct
     3001 gagaaaaggt atgttcaagt cataagtagg taattgatag ggtatgaact agtcaaaata
     3061 tgaaccatta tgattcaagt tagattttcc tctggagaga cagatctgaa tgttcagttc
     3121 tagccaaggt agattttact ttcaactttt taatcagtat cactttctgt gcttaactat
     3181 ttggtgttac cttgtctgtt ttcatttgtc taaaattctg cagagatgac taaaatttga
     3241 cataatggtg taaatggatt gttaaggtaa ctttcagttg aagattaaag caagatgcca
     3301 ttttccccat gactttggtt ttgtttacat ttttcccttt cagttagtat acactacacg
     3361 tactgtaata aaatacaata atatga
//
