LOCUS       NM_015568               6259 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens protein phosphatase 1 regulatory subunit 16B
            (PPP1R16B), transcript variant 1, mRNA.
ACCESSION   NM_015568
VERSION     NM_015568.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Fonodi,M., Thalwieser,Z., Csortos,C. and Boratko,A.
  TITLE     TIMAP, a Regulatory Subunit of Protein Phosphatase 1, Inhibits In
            Vitro Neuronal Differentiation
  JOURNAL   Int J Mol Sci 24 (24), 17360 (2023)
   PUBMED   38139189
  REMARK    GeneRIF: TIMAP, a Regulatory Subunit of Protein Phosphatase 1,
            Inhibits In Vitro Neuronal Differentiation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 6259)
  AUTHORS   Quan,X., Liu,X., Qin,X., Wang,Y., Sun,T., Li,Z., Zhu,L., Chen,J.,
            Zhou,Y., Singh,S., Dong,H., Zhang,Z. and Zhang,H.
  TITLE     The role of LR-TIMAP/PP1c complex in the occurrence and development
            of no-reflow
  JOURNAL   EBioMedicine 65, 103251 (2021)
   PUBMED   33639401
  REMARK    GeneRIF: The role of LR-TIMAP/PP1c complex in the occurrence and
            development of no-reflow.
REFERENCE   4  (bases 1 to 6259)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 6259)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 6259)
  AUTHORS   Czikora,I., Kim,K.M., Kasa,A., Becsi,B., Verin,A.D., Gergely,P.,
            Erdodi,F. and Csortos,C.
  TITLE     Characterization of the effect of TIMAP phosphorylation on its
            interaction with protein phosphatase 1
  JOURNAL   Biochimie 93 (7), 1139-1145 (2011)
   PUBMED   21466834
  REMARK    GeneRIF: Data suggest that double-thiophosphorylation of TIMAP has
            minor effect on its binding ability to PP1c, but considerably
            attenuates its inhibitory effect on the activity of PP1c.
REFERENCE   7  (bases 1 to 6259)
  AUTHORS   Csortos,C., Czikora,I., Bogatcheva,N.V., Adyshev,D.M., Poirier,C.,
            Olah,G. and Verin,A.D.
  TITLE     TIMAP is a positive regulator of pulmonary endothelial barrier
            function
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 295 (3), L440-L450 (2008)
   PUBMED   18586956
  REMARK    GeneRIF: involvement of TIMAP in PKA-mediated moesin
            dephosphorylation and the importance of this dephosphorylation in
            TIMAP-mediated endothelial cell barrier protection
REFERENCE   8  (bases 1 to 6259)
  AUTHORS   Kim,K., Li,L., Kozlowski,K., Suh,H.S., Cao,W. and Ballermann,B.J.
  TITLE     The protein phosphatase-1 targeting subunit TIMAP regulates LAMR1
            phosphorylation
  JOURNAL   Biochem Biophys Res Commun 338 (3), 1327-1334 (2005)
   PUBMED   16263087
REFERENCE   9  (bases 1 to 6259)
  AUTHORS   Homma,K., Kikuno,R.F., Nagase,T., Ohara,O. and Nishikawa,K.
  TITLE     Alternative splice variants encoding unstable protein domains exist
            in the human brain
  JOURNAL   J Mol Biol 343 (5), 1207-1220 (2004)
   PUBMED   15491607
REFERENCE   10 (bases 1 to 6259)
  AUTHORS   Cao,W., Mattagajasingh,S.N., Xu,H., Kim,K., Fierlbeck,W., Deng,J.,
            Lowenstein,C.J. and Ballermann,B.J.
  TITLE     TIMAP, a novel CAAX box protein regulated by TGF-beta1 and
            expressed in endothelial cells
  JOURNAL   Am J Physiol Cell Physiol 283 (1), C327-C337 (2002)
   PUBMED   12055102
  REMARK    GeneRIF: TIMAP, a novel CAAX box protein regulated by TGF-beta1 and
            expressed in endothelial cells.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY125679.1, AB020630.2,
            AB177855.1 and BC152467.1.
            
            On Nov 22, 2018 this sequence version replaced NM_015568.3.
            
            Summary: The protein encoded by this gene is membrane-associated
            and contains five ankyrin repeats, a protein
            phosphatase-1-interacting domain, and a carboxy-terminal CAAX box
            domain. Synthesis of the encoded protein is inhibited by
            transforming growth factor beta-1. The protein may bind to the
            membrane through its CAAX box domain and may act as a signaling
            molecule through interaction with protein phosphatase-1.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms that may undergo similar processing to
            generate mature protein. [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC152467.1, AB020630.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299824.6/ ENSP00000299824.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 HY125679.1         7-14
            9-3321              AB020630.2         1-3313
            3322-6252           AB177855.1         2827-5757
            6253-6259           BC152467.1         6245-6251
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.23"
     gene            1..6259
                     /gene="PPP1R16B"
                     /gene_synonym="ANKRD4; TIMAP"
                     /note="protein phosphatase 1 regulatory subunit 16B"
                     /db_xref="GeneID:26051"
                     /db_xref="HGNC:HGNC:15850"
                     /db_xref="MIM:613275"
     exon            1..96
                     /gene="PPP1R16B"
                     /gene_synonym="ANKRD4; TIMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            97..447
                     /gene="PPP1R16B"
                     /gene_synonym="ANKRD4; TIMAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             198..1901
                     /gene="PPP1R16B"
                     /gene_synonym="ANKRD4; TIMAP"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; TGF-beta-inhibited membrane-associated protein;
                     CAAX box protein TIMAP; ankyrin repeat domain protein 4;
                     hTIMAP; ankyrin repeat domain-containing protein 4;
                     protein phosphatase 1, regulatory (inhibitor) subunit 16B"
                     /codon_start=1
                     /product="protein phosphatase 1 regulatory inhibitor
                     subunit 16B isoform 1 precursor"
                     /protein_id="NP_056383.1"
                     /db_xref="CCDS:CCDS13309.1"
                     /db_xref="GeneID:26051"
                     /db_xref="HGNC:HGNC:15850"
                     /db_xref="MIM:613275"
                     /translation="MASHVDLLTELQLLEKVPTLERLRAAQKRRAQQLKKWAQYEQDL
                     QHRKRKHERKRSTGGRRKKVSFEASVALLEASLRNDAEEVRYFLKNKVSPDLCNEDGL
                     TALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHAAATCGHINLVKILVQYGADL
                     LAVNSDGNMPYDLCEDEPTLDVIETCMAYQGITQEKINEMRVAPEQQMIADIHCMIAA
                     GQDLDWIDAQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQMQ
                     MAELLVSHGASLSARTSMDEMPIDLCEEEEFKVLLLELKHKHDVIMKSQLRHKSSLSR
                     RTSSAGSRGKVVRRASLSDRTNLYRKEYEGEAILWQRSAAEDQRTSTYNGDIRETRTD
                     QENKDPNPRLEKPVLLSEFPTKIPRGELDMPVENGLRAPVSAYQYALANGDVWKVHEV
                     PDYSMAYGNPGVADATPPWSSYKEQSPQTLLELKRQRAAAKLLSHPFLSTHLGSSMAR
                     TGESSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPIEEMEEKVHGCCRI
                     S"
     exon            448..518
                     /gene="PPP1R16B"
                     /gene_synonym="ANKRD4; TIMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            519..664
                     /gene="PPP1R16B"
                     /gene_synonym="ANKRD4; TIMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            665..768
                     /gene="PPP1R16B"
                     /gene_synonym="ANKRD4; TIMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            769..893
                     /gene="PPP1R16B"
                     /gene_synonym="ANKRD4; TIMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1019
                     /gene="PPP1R16B"
                     /gene_synonym="ANKRD4; TIMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1095
                     /gene="PPP1R16B"
                     /gene_synonym="ANKRD4; TIMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1225
                     /gene="PPP1R16B"
                     /gene_synonym="ANKRD4; TIMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1391
                     /gene="PPP1R16B"
                     /gene_synonym="ANKRD4; TIMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..6259
                     /gene="PPP1R16B"
                     /gene_synonym="ANKRD4; TIMAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6239..6244
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP1R16B"
                     /gene_synonym="ANKRD4; TIMAP"
                     /note="hexamer: ATTAAA"
     polyA_site      6259
                     /gene="PPP1R16B"
                     /gene_synonym="ANKRD4; TIMAP"
                     /note="major polyA site"
ORIGIN      
        1 agagccagct ctgtcgccgc tggagcagct gcggctcggg gacccacaga cacagccggg
       61 gtcgggagcc gcccggggca gggctcggga gagcgggcca caccatgagg ccccagcccc
      121 accagaggcc ccgcgctgcc ctggcccccg gtgcaccgtg ctagccccca gccagggcgt
      181 tggggagggc ggtggccatg gccagtcacg tggacctgct gacggagctg cagctgctgg
      241 agaaggtgcc cacgctggag cggctgcggg ctgcccagaa gcgccgggcc cagcagctga
      301 agaaatgggc acagtacgag caggacttgc agcaccgcaa gcgaaagcat gagcggaagc
      361 gcagcacggg cggccgccgc aagaaagtgt ccttcgaggc cagcgtggcc ctgctggagg
      421 cctcgctgag gaacgacgcc gaggaagtac gctacttcct gaagaataag gtcagccctg
      481 atttgtgcaa tgaggacgga ctcacagccc tacaccagtg ctgcatcgac aactttgagg
      541 aaattgtgaa gctgctcctc tcccatggtg ccaatgtgaa cgccaaggac aacgagctgt
      601 ggacacctct ccatgctgca gccacctgcg gccacatcaa cctggtgaag atcctcgttc
      661 agtatggggc cgacttgctt gctgtcaact cggatgggaa catgccatat gacctctgcg
      721 aggatgaacc caccctggat gtcatcgaga cctgcatggc ataccagggc atcacccaag
      781 agaaaatcaa cgagatgcgg gtggctcctg agcagcagat gattgcggac atccactgca
      841 tgatcgcagc gggccaggac ctggactgga tagatgccca gggtgccaca ctgctgcaca
      901 tagctggagc caatggatac ctgcgggcag ctgagctcct cctggaccat ggagtgcgtg
      961 tggatgtgaa ggactgggat ggctgggagc ccctgcatgc agctgccttc tggggacaga
     1021 tgcagatggc agagctattg gtgtcccatg gagctagtct cagtgcaagg acatccatgg
     1081 atgagatgcc aatagacctg tgcgaggagg aagagttcaa ggtcctgctg ctggagctaa
     1141 aacacaagca tgatgtgatc atgaagtcac agctgaggca caagtcatcc ttgagccgga
     1201 ggacctccag cgcaggcagc cgtgggaagg tggtgcggcg agccagcctg tcggacagga
     1261 ccaacctgta taggaaggag tatgagggag aggccatcct gtggcagcgg agtgcagctg
     1321 aggatcagcg gacctccacc tacaacgggg acatcaggga gaccaggaca gaccaagaga
     1381 ataaggaccc taaccccagg ctggagaagc ccgtgctact ctccgaattt cctaccaaga
     1441 tcccacgagg tgaactggac atgcctgttg agaatggcct ccgggctccg gtcagtgcct
     1501 accagtatgc gctggccaac ggggatgtct ggaaggtgca tgaggtgcct gactacagca
     1561 tggcctatgg caaccctggc gtggccgacg ccaccccgcc ctggagcagc tacaaggaac
     1621 agagccctca gacgcttctg gagctgaagc ggcagcgggc tgcagccaag ctgctcagcc
     1681 accccttcct tagcacacac ctgggcagca gcatggccag gacgggcgag agtagcagtg
     1741 aaggcaaggc ccccttgatc ggaggcagaa cttcaccgta cagcagcaat gggacctcgg
     1801 tatattacac ggtcaccagc ggagatcccc cactcttaaa gttcaaggcc cccatagagg
     1861 agatggagga gaaggtgcat ggctgttgcc gtatctccta gtctccgtgt gatggaggag
     1921 ggagatgcct ggggaggggc tcctggaatc caggccagcc caacagccct ggctggggag
     1981 gtgtcagggc agctggggag aggtgggctc tgcttttcag aggaactcag accccagccc
     2041 tcagctggct gcccatagca tcccatgtcc cacgtcccgt ggttctgctt cctgctgcat
     2101 cgtctgccat ctgacacaag gcctgtcgtg gcctcctggt tcactctgct gtctgatctt
     2161 gggagggtgg gcttgagatc ccagctctat tcttggtata aaggcttctc cggatcagta
     2221 catgcatgtc acattaacac acacacacac acacatatac acacacacac aagctcgatc
     2281 agtgtgtgta ggaatgacat acctgggctc aggggaagca agggggctta gaatttgtgg
     2341 ggtattccca aaaggatgga agttaagact cagagtctca ttaccactgc caatgtggtt
     2401 ttagcagggg aggggacctg ctaagctgag acccatagtc ctgctcagag ttatcccaaa
     2461 gtctgagcca ccagccacac ctgacagggg tgagaagtcc tcgctgtgtt cagagggagc
     2521 caggaatcta catgggtaga tgagatagac acagacctgc tccccgcagc cttgttgaga
     2581 gccacacttc tgcccatgcc aggagccagc tgtgtgacca tccaggggtg gagggggaaa
     2641 accaggcaat ttcgttcctg gaatcaacca aatcatgttt tcctcttgga tggaagtgtc
     2701 aaaggcagaa gggtgtggga gggggacaag gtcagtattt accaaagtgt atctgatttt
     2761 aaaaattcct ttagtctgta aaactcctag agggagggag gtaactgaat tcacttcttt
     2821 ttgtggatcg tatcaaggtc actgggtttt actggctggt gctgggaaaa tgaagctaag
     2881 tgaggagctt ccattggaat gcttttccag ggagagaggc cagttaattt aaaaaaaaca
     2941 gtcgctagtt aacagcgaca gagcccagca ccctggggtc tttgtgaata tccagactgt
     3001 ttcagcccag cccatctcag ccaaccctcc ttagactgag ctgtcagagc aagcaattag
     3061 gggccagcct gcctccacct cccaccccct tccacctcca tcagtcatgt gtgcagagtc
     3121 agtgctcggg atcccgggcc cagcttttgc ctttttgggg atgcttggtg agacagattt
     3181 gccagtcagc ccttttgagt tcccgcctca cccaggggct cccagcctgc acttgcagga
     3241 gtggtgatgc cccaagtctg cgaatccagg gtgcacgtgg tcaatatccc ctcctgcatt
     3301 caggagagcc atggtagggc tagagttggg tcttgcccag ccctgcagtt tcatagtccc
     3361 agccttcctg gtgctgggga gggaggactg tgaatggctg ttctcccctc actgctgagt
     3421 ctcccaggac cccctttgga gatgcccatg gcatgggcac tgcccacagg ctcagccaga
     3481 acctcttggt gtacccgata agctgcaggt tatcccttgc tctgtgcgcc ttttatttgt
     3541 ccttaaacta cctccttaga gctctgaagg ggtctcctag ttccagattt taatttgggg
     3601 aacagatctg ggttcttttt aaccctcttc tttctcagtc tatgagaaac ttgccctgag
     3661 gggcacctgg gctaggggct tgggactgga agaccatccc cgccttgtgc cacaactttg
     3721 gtcatgggat ctgctctttg tcattcttag ccccctactg tggcccccat agccccataa
     3781 cccagagagg gagctggact tcagggagcc tgagtgatgc tttcccagga gcagggcagc
     3841 tggctggacc agaaagtaga gggcccatgg gagtgactgc acccttggtg gctgctggaa
     3901 ggggagaggt tctcagcatc aggccacctc caccccaatg ccaggataga tgtattctag
     3961 agtaggggtg gaggcggccc aggaggctga agacaggtgc acagatgctt cccacgacct
     4021 tgccatttgg ggtgggctct tcaacatctc aggctgtggc tggaacagga caggatgatc
     4081 taaaacacac gtaccattgg ctgtaaaaca gtatgagccc agactgacgc tgaaatccct
     4141 catgagccaa ccttagctac aaggtaggga gttctgaggg aagccgcgtg ctcctcagga
     4201 gagagctgtt taggttttcc gatctttttg ctcaggggcc aaacactgaa ggcacgtact
     4261 gcccaaccca ctgagcgcct gaggccattc cctccttttc cgcatgcctc ctgcctcctg
     4321 ggctattcct ctccacccag aaggctggga atcccagctg attccctgac aggagccgac
     4381 ttcacacaca ggtgactctc aggcattggc tcatgttttc agccagggat aaaccatccc
     4441 ttcttggggc tttaagtccc tggggagctt tccctgtagg tctcctgggt gttgagagac
     4501 aagttggaga ccaacctcca atgaatgagc cgcggtcatt cattaattca ctcacgtaat
     4561 ttactgagta gctgcaacat gccagcctct acgttaggtt ctgcggataa aggaggaata
     4621 agacagagtc aggagaactg ttccttgtgg tttccgtccc ttggggacca caggcatcag
     4681 cagtcccatt caagtcacct gaggcaaagt gtctgcatct tcgtccagcg accctttgct
     4741 tttcggctcc tagaatcctt agagtctgaa ttcctttagc tgggaacagc tgtcatggtc
     4801 acccctggat aacatttgcc accaagtata gatgctggat cttgggttcc aggcagacat
     4861 catccaggtc catctggaac tttcagtgat agctgccttc agccagcatc tttgggggac
     4921 tctataatag cagcttgaga tcagtgtcta gaagactgtt ctgcaatttg ctgccaaatg
     4981 catctcaggt ttttaaagtc attgtttctt gctcatggtg gctcatttat tacatagtcc
     5041 cctcacccca ctaatggata atgggaggaa aagttgctgc ttccttcagc atcaaagcct
     5101 ttccttggga atctgcctcc ctccatggca ggggtggatt cgggagctgg gagtaaccag
     5161 gcaaagtcaa ccagatgcct agctcctgct gagacccagg tcctatggca gctcctcatt
     5221 agattaaagg agaccacttc caaagcaggt gctgcatggc tcaccatcat atgccccaaa
     5281 caactgaaag ttggcggtta tcaccagact gtgagtttct ggcaagtagc ttggggaagc
     5341 tgaataaact ctaggcccag ggctactaaa gacttcagga tagaattctc catcaaatat
     5401 acagcataag taaaactgct ctgcactgtt taatccattt ccaaggggct tagaaaagct
     5461 aacaagggtg tgtcccctgt cctgccccac cggtttgctg gctttgtaat aacataagac
     5521 cattgtggtt gttggtgtca gataccttcc catcctgagc tctctcacct acctgctctc
     5581 tctcctagag caggatactg gggtactttt aagaagggtg ctccttttaa gatgcccaga
     5641 aaagctgtat ttaactcttg ctatttgtaa cttggggatg gtctcccctg ccccagggca
     5701 cataagagca aaggctccaa tggtcagtgg atgactctgc aaaagtgacc ccctgtgcca
     5761 gaagctatag ccctctcccc aacaggtctc tcttgttggc cagagggcct gcttcccatg
     5821 ggcattgcaa gtgccaccgt gcggggcctg gctctgcaca cccaggaaaa gtctgcagac
     5881 ccccagccct ccgcaataat tcaccagacc agaagccact ggtgtacaga gaacacttaa
     5941 aaaaatgtat tttatgtgaa aaaaaattaa aactctgtat actgtatcag cagctttgtg
     6001 taaaaatggc aatcaagaga gtctaatata tttaaaactt ttttaaaaaa aatcttcgca
     6061 gatctttgat atcgtactga ggtaacttcc acgtagcccc ttgccacgcg gcaccggtgg
     6121 gccttgggtc caaaactgtg gctcagccac atcccaaagg gggcacatgt ccctggagtt
     6181 gcttccagct gccaaggcct gtgacagaat tcgctgttaa gagtttttaa ttaaaattat
     6241 taaattcctt ttaataaca
//
