LOCUS       NM_032833               5259 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens protein phosphatase 1 regulatory subunit 15B
            (PPP1R15B), mRNA.
ACCESSION   NM_032833
VERSION     NM_032833.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Fatalska,A., Hodgson,G., Freund,S.M.V., Maslen,S.L., Morgan,T.,
            Thorkelsson,S.R., van Slegtenhorst,M., Lorenz,S., Andreeva,A.,
            Kaat,L.D. and Bertolotti,A.
  TITLE     Recruitment of trimeric eIF2 by phosphatase non-catalytic subunit
            PPP1R15B
  JOURNAL   Mol Cell 84 (3), 506-521 (2024)
   PUBMED   38159565
  REMARK    GeneRIF: Recruitment of trimeric eIF2 by phosphatase non-catalytic
            subunit PPP1R15B.
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Hicks,D., Giresh,K., Wrischnik,L.A. and Weiser,D.C.
  TITLE     The PPP1R15 Family of eIF2-alpha Phosphatase Targeting Subunits
            (GADD34 and CReP)
  JOURNAL   Int J Mol Sci 24 (24), 17321 (2023)
   PUBMED   38139150
  REMARK    GeneRIF: The PPP1R15 Family of eIF2-alpha Phosphatase Targeting
            Subunits (GADD34 and CReP).
            Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5259)
  AUTHORS   Gu,F., Xu,F., Pan,Z., Shi,L., Yu,J., Song,F., Huang,S. and Sun,M.
  TITLE     An integrative pan-cancer analysis illustrating the key role of
            LRP11 in cervical cancer
  JOURNAL   Medicine (Baltimore) 102 (11), e33201 (2023)
   PUBMED   36930084
  REMARK    GeneRIF: An integrative pan-cancer analysis illustrating the key
            role of LRP11 in cervical cancer.
REFERENCE   4  (bases 1 to 5259)
  AUTHORS   Hodgson,G., Andreeva,A. and Bertolotti,A.
  TITLE     Substrate recognition determinants of human eIF2alpha phosphatases
  JOURNAL   Open Biol 11 (12), 210205 (2021)
   PUBMED   34847777
  REMARK    GeneRIF: Substrate recognition determinants of human eIF2alpha
            phosphatases.
REFERENCE   5  (bases 1 to 5259)
  AUTHORS   Chen,R., Rato,C., Yan,Y., Crespillo-Casado,A., Clarke,H.J.,
            Harding,H.P., Marciniak,S.J., Read,R.J. and Ron,D.
  TITLE     G-actin provides substrate-specificity to eukaryotic initiation
            factor 2alpha holophosphatases
  JOURNAL   Elife 4, e04871 (2015)
   PUBMED   25774600
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5259)
  AUTHORS   Andreev,D.E., O'Connor,P.B., Fahey,C., Kenny,E.M., Terenin,I.M.,
            Dmitriev,S.E., Cormican,P., Morris,D.W., Shatsky,I.N. and
            Baranov,P.V.
  TITLE     Translation of 5' leaders is pervasive in genes resistant to eIF2
            repression
  JOURNAL   Elife 4, e03971 (2015)
   PUBMED   25621764
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5259)
  AUTHORS   Kloft,N., Neukirch,C., von Hoven,G., Bobkiewicz,W., Weis,S.,
            Boller,K. and Husmann,M.
  TITLE     A subunit of eukaryotic translation initiation factor
            2alpha-phosphatase (CreP/PPP1R15B) regulates membrane traffic
  JOURNAL   J Biol Chem 287 (42), 35299-35317 (2012)
   PUBMED   22915583
  REMARK    GeneRIF: the mammalian traffic machinery co-opts p-eIF2alpha and
            CReP, regulators of translation initiation.
REFERENCE   8  (bases 1 to 5259)
  AUTHORS   Fardilha,M., Esteves,S.L., Korrodi-Gregorio,L., Vintem,A.P.,
            Domingues,S.C., Rebelo,S., Morrice,N., Cohen,P.T., da Cruz e
            Silva,O.A. and da Cruz e Silva,E.F.
  TITLE     Identification of the human testis protein phosphatase 1
            interactome
  JOURNAL   Biochem Pharmacol 82 (10), 1403-1415 (2011)
   PUBMED   21382349
REFERENCE   9  (bases 1 to 5259)
  AUTHORS   Harding,H.P., Zhang,Y., Scheuner,D., Chen,J.J., Kaufman,R.J. and
            Ron,D.
  TITLE     Ppp1r15 gene knockout reveals an essential role for translation
            initiation factor 2 alpha (eIF2alpha) dephosphorylation in
            mammalian development
  JOURNAL   Proc Natl Acad Sci U S A 106 (6), 1832-1837 (2009)
   PUBMED   19181853
REFERENCE   10 (bases 1 to 5259)
  AUTHORS   Ceulemans,H., Stalmans,W. and Bollen,M.
  TITLE     Regulator-driven functional diversification of protein
            phosphatase-1 in eukaryotic evolution
  JOURNAL   Bioessays 24 (4), 371-381 (2002)
   PUBMED   11948623
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL606489.26 and KF459739.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 27, 2020 this sequence version replaced NM_032833.4.
            
            Summary: This gene encodes a protein phosphatase I-interacting
            protein that promotes the dephosphorylation of eukaryotic
            translation initiation factor 2A to regulate translation under
            conditions of cellular stress. The transcribed messenger RNA
            contains two upstream open reading frames (ORFs) that repress
            translation of the main protein coding ORF under normal conditions,
            while the protein coding ORF is expressed at high levels in
            response to stress. Continual translation of the mRNA under
            conditions of eukaryotic translation initiation factor 2A
            inactivation is thought to create a feedback loop for reactivation
            of the gene during recovery from stress. In addition, it has been
            shown that this protein plays a role in membrane traffic that is
            independent of translation and that it is required for exocytosis
            from erythroleukemia cells. Allelic variants of this gene are
            associated with microcephaly, short stature, and impaired glucose
            metabolism. [provided by RefSeq, Feb 2016].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK027650.1, BC065280.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367188.5/ ENSP00000356156.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            regulatory uORF        :: PMID: 25621764
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1144              AL606489.26        62632-63775         c
            1145-1517           KF459739.1         1-373
            1518-2326           AL606489.26        61450-62258         c
            2327-5259           AL606489.26        55339-58271         c
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.1"
     gene            1..5259
                     /gene="PPP1R15B"
                     /gene_synonym="CREP; MSSGM2"
                     /note="protein phosphatase 1 regulatory subunit 15B"
                     /db_xref="GeneID:84919"
                     /db_xref="HGNC:HGNC:14951"
                     /db_xref="MIM:613257"
     exon            1..2326
                     /gene="PPP1R15B"
                     /gene_synonym="CREP; MSSGM2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      128..154
                     /regulatory_class="uORF"
                     /gene="PPP1R15B"
                     /gene_synonym="CREP; MSSGM2"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:25621764]"
                     /note="this uORF is predicted to encode an 8 aa peptide
                     (PMID:25621764)"
     regulatory      176..331
                     /regulatory_class="uORF"
                     /gene="PPP1R15B"
                     /gene_synonym="CREP; MSSGM2"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:25621764]"
                     /note="this uORF is predicted to encode a 51 aa peptide
                     (PMID:25621764)"
     CDS             407..2548
                     /gene="PPP1R15B"
                     /gene_synonym="CREP; MSSGM2"
                     /note="protein phosphatase 1, regulatory (inhibitor)
                     subunit 15B"
                     /codon_start=1
                     /product="protein phosphatase 1 regulatory subunit 15B"
                     /protein_id="NP_116222.4"
                     /db_xref="CCDS:CCDS1445.1"
                     /db_xref="GeneID:84919"
                     /db_xref="HGNC:HGNC:14951"
                     /db_xref="MIM:613257"
                     /translation="MEPGTGGSRKRLGPRAGFRFWPPFFPRRSQAGSSKFPTPLGPEN
                     SGNPTLLSSAQPETRVSYWTKLLSQLLAPLPGLLQKVLIWSQLFGGMFPTRWLDFAGV
                     YSALRALKGREKPAAPTAQKSLSSLQLDSSDPSVTSPLDWLEEGIHWQYSPPDLKLEL
                     KAKGSALDPAAQAFLLEQQLWGVELLPSSLQSRLYSNRELGSSPSGPLNIQRIDNFSV
                     VSYLLNPSYLDCFPRLEVSYQNSDGNSEVVGFQTLTPESSCLREDHCHPQPLSAELIP
                     ASWQGCPPLSTEGLPEIHHLRMKRLEFLQQASKGQDLPTPDQDNGYHSLEEEHSLLRM
                     DPKHCRDNPTQFVPAAGDIPGNTQESTEEKIELLTTEVPLALEEESPSEGCPSSEIPM
                     EKEPGEGRISVVDYSYLEGDLPISARPACSNKLIDYILGGASSDLETSSDPEGEDWDE
                     EAEDDGFDSDSSLSDSDLEQDPEGLHLWNSFCSVDPYNPQNFTATIQTAARIVPEEPS
                     DSEKDLSGKSDLENSSQSGSLPETPEHSSGEEDDWESSADEAESLKLWNSFCNSDDPY
                     NPLNFKAPFQTSGENEKGCRDSKTPSESIVAISECHTLLSCKVQLLGSQESECPDSVQ
                     RDVLSGGRHTHVKRKKVTFLEEVTEYYISGDEDRKGPWEEFARDGCRFQKRIQETEDA
                     IGYCLTFEHRERMFNRLQGTCFKGLNVLKQC"
     exon            2327..5259
                     /gene="PPP1R15B"
                     /gene_synonym="CREP; MSSGM2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5229..5234
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP1R15B"
                     /gene_synonym="CREP; MSSGM2"
                     /note="hexamer: AATAAA"
     regulatory      5248..5253
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP1R15B"
                     /gene_synonym="CREP; MSSGM2"
                     /note="hexamer: ATTAAA"
     polyA_site      5248
                     /gene="PPP1R15B"
                     /gene_synonym="CREP; MSSGM2"
                     /note="major polyA site"
     polyA_site      5259
                     /gene="PPP1R15B"
                     /gene_synonym="CREP; MSSGM2"
                     /note="major polyA site"
ORIGIN      
        1 attttgggct tcgcttccac cgcaccagcc ggcctaccca gtccttccgg tatcgcgttg
       61 ctcaggggct tttcaaccct ctgtcagtcg gaaaaccatc gccgaggccg tggggggact
      121 cctatccatg gtgttgaagc gtcgagccga ctagggaacc tccttccccg ccaggatgga
      181 agtcgcatca gtcgccgcct attgcgcggg ctgttcttcc ctgtgttctg ccgcccgctg
      241 ccgcattcgc tgccctctgt ggcttttctg ctggctcgaa gatcggcctg gagcagcgac
      301 gccaccgctg ggcaaggccg agactctgta ggcttcctcc gaatcccgtc gacctccagc
      361 cgctgagcgc cgcggcccta cctgagagac tgtcaagaaa aaggagatgg agccggggac
      421 aggcggatcg cggaaacggc ttggccctcg ggcgggcttc cggttctggc cacccttttt
      481 ccctcggcga tcgcaagcag gctcttctaa gttcccgacg cctcttggcc cggaaaactc
      541 cgggaacccc acactgcttt cctctgccca gcccgagact cgggtcagtt actggacgaa
      601 actgctctcc cagctccttg cgccgctccc cggattgctt cagaaggtgc taatttggag
      661 ccaacttttc ggtggaatgt ttccgaccag atggctagat tttgctggag tctacagcgc
      721 cctgagagcc ctgaagggac gggagaaacc agccgccccc acagcgcaga aatctttgag
      781 ttcgctgcag ctcgactcct cagacccctc ggtcaccagt ccccttgatt ggctagagga
      841 ggggatccac tggcaatact cgcccccaga cctaaaattg gagcttaagg ccaagggaag
      901 tgctttggac cctgcagcac aggcttttct cttagagcag cagctgtggg gagtggagct
      961 gttgcccagt agccttcaat cccgtctgta ctctaaccgg gaacttggct cttcgccctc
     1021 tgggcctcta aacattcaac gcatagacaa tttcagtgtg gtatcctatt tgctgaaccc
     1081 ttcctacctg gactgctttc ctaggctaga agtcagctat cagaacagtg atggaaatag
     1141 cgaggtagtc ggcttccaga cactaacccc agagagcagc tgcctgagag aggaccattg
     1201 tcatccccag ccgctgagtg cagaactcat tccggcctcg tggcagggat gtccacctct
     1261 ttctacggaa ggcctaccag aaattcacca tcttcgcatg aaacggctgg aattccttca
     1321 acaggctagc aaggggcaag atttacccac ccctgaccag gataatggct accacagcct
     1381 ggaggaggaa cacagccttc tccggatgga tccaaaacac tgcagagata acccaacaca
     1441 gtttgttcct gctgctggag acattcctgg aaacacccag gaatccactg aagaaaaaat
     1501 agaattatta actacagagg ttccacttgc tttggaagaa gagagccctt ctgagggctg
     1561 tccatctagt gagataccta tggaaaagga gcctggagag ggccgaataa gtgtagttga
     1621 ttactcatac ctagaaggtg accttcccat ttctgccaga ccagcttgta gtaacaaact
     1681 gatagattat attttgggag gtgcatccag tgacctggaa acaagttctg atccagaagg
     1741 tgaggattgg gatgaggaag ctgaggatga tggttttgat agtgatagct cactgtcaga
     1801 ctcagacctt gaacaagacc ctgaagggct tcacctttgg aactctttct gcagtgtaga
     1861 tccttataat ccccagaact ttacagcaac aattcagact gctgccagaa ttgttcctga
     1921 agagccttct gattcagaga aggatttgtc tggcaagtct gatctagaga attcctccca
     1981 gtctggaagc cttcctgaga cccctgagca tagttctggg gaggaagatg actgggaatc
     2041 tagtgcagat gaagcagaga gtctcaaact gtggaactca ttctgtaatt ctgatgaccc
     2101 ctacaaccct ttaaatttta aggctccttt tcaaacatca ggggaaaatg agaaaggctg
     2161 tcgtgactca aagaccccat ctgagtccat tgtggccatt tctgagtgtc acaccttact
     2221 ttcttgtaag gtgcagctgt tggggagcca agaaagtgaa tgtccagact cggtacagcg
     2281 tgacgttctt tctggaggaa gacacacaca tgtcaaaaga aaaaaggtaa ccttccttga
     2341 agaagttact gagtattata taagtggtga tgaggatcgc aaaggaccat gggaagaatt
     2401 tgcaagggat ggatgcaggt tccagaaacg aattcaagaa acagaagatg ctattggata
     2461 ttgcttgaca tttgaacaca gagaaagaat gtttaataga ctccagggaa catgcttcaa
     2521 aggacttaat gttctcaagc aatgttgagt tggcagcctg tagtcctagc tagcatacac
     2581 tacctcttac ctgagaggtg tcttttaaaa acaaatcttg gcagctgtcc tttgacattt
     2641 ttttttttag aggaaatgta acttggatct agtttaattt ttttttttgc aacatatccc
     2701 actcagaaac attcaggttt gaagccagcc ctgataatga aggatgaact agtgtgattt
     2761 ctaatcctcc cttttttgat ttagttggat gtgcttttaa atgtcctttg cctgcatgag
     2821 gtggaaaggg gacctttttg agttgtcatt ttgcactttc aaaacttatt ttcttggaaa
     2881 acaatattta tagggcttaa agcccatttt catttctaat ctaaattatg tgtgcctatc
     2941 tgaaaacttt gggctctttc ttgtttcttt cccaaaattc agaagttaat gggcttttat
     3001 gtttttctat atttttttta tttcaatgat ttggcctgtc tatgttaggc taaaaaataa
     3061 ccttgtgtat gctaccaact taaagtgcat tattttgtgt cacttttttt tttcttgtaa
     3121 aaatgacttg gattgaaaat atgtggtagc ctttttattt ctacattaag ttctacctag
     3181 gatatttcca aggactgcca caaaacccat atgtgcagta ctttactact ttgggaaagc
     3241 tgcatctttc taccacattt taacatctaa tatatttaat ttctttgaag agggttctgt
     3301 gtacgttatt gtagttccca gtttaatata gttctttgta tctcttaaca ggttgaagtt
     3361 attgcaaaac actctggaaa gtaataatta catcataatc atttattttt taaacttaaa
     3421 agcctagaaa tttcctagaa agaaaatagg agacatctca gagcaatttg gttttggtgt
     3481 atatgttctc aacagaaaac cagtgttaat gaatatcatg cctcagcact gtcactttta
     3541 aaacctgtca ggatcccact gtaaaattgg aaatgggcag ttctgaattt tcacgtttga
     3601 aatgtaaaat ataaacttca gtcaatatcc aggtttattg tgtcctacta tttaataatg
     3661 agagaagtaa tggcaaggcc tttactttca ggaaaggata gaagtataga ttaatgactg
     3721 gaaagtttta atatatttag cccaaaggtt actttgaatt gaagtctttg cattgactgt
     3781 ttgtgtttgg tttatttgtt tagctttaca aggtacacat aagttaggtt gaggggttgt
     3841 taacccttcc gtggtctgct ttcattccgt gtgcttcctg tcacaggtaa tggaaaacat
     3901 aagtagaata ggtgacctct tagttttgaa cttatttaag tgtggggatg aatttttcat
     3961 cagaagtgct tacagggtta ctacctcagt ttacaatcta cctggtcatt attttatttc
     4021 tatccagttc taagaactgc ctccactgtt tatatattca taattaaaca cattgagaat
     4081 gcaacactat aaaagctggt caaatttttg cagagccctt attctgtgtg ttttttgttt
     4141 ttttcttttt tttttgagac agagtctcgt tcggtccccc agcttggagt gcagtggcgc
     4201 gatctcggct cactgcaacc tccgcctcct gggttcacgc gattctcctg cctcagcctc
     4261 ccgagtagct gggattacag gcacacacca ccacgcccgg ctaatttttt gtgtcttttt
     4321 agtagagacg gggtttcgct atgttggcca gactggtctt gaactgctga cctcgtgatc
     4381 cgcccgcctc ggcctctcaa agtgctggga ttctgtgtgt tttgtgcacc tccactttag
     4441 gtaatcatag ggagcacatt tacaggatgg tctaataaca tgaaaacagg ctagtttcaa
     4501 gcaacagcaa tgtcggttgg aaagcaggcg tcatttgcct tgaaaaaagc cttttgacaa
     4561 catacaggca ttcttttaaa accaggctga aacattttat ttccgagact taacgttgtg
     4621 tttcctgttt cttaaaccta gcacctctgt gtatttgaaa ataatgagac atctttcatt
     4681 ggattttgga aaattgttcc ccatgggatt ctaacctcac taccaaatga gtgaaagctt
     4741 gattaagagt tcttccatat actagcctcc ttggaagaag tgatcagaag gtgataagaa
     4801 ggacagaaag gactatttta aagttggact gaaggagaaa aaagcaaaat tcttgtttca
     4861 tcccaattct agttagaaca aagttaaacc cccgtaatct taaagagaaa atctttggag
     4921 gttttaatta aacattttat acatttaaag tcttgttaat ggtgctttaa gtgtcaatgt
     4981 agcatgtaaa aggctttgta cagacaggta aaagttccat ttctgagtga tgaaatgtaa
     5041 cacttcttca tctttaactt gaaatcaaaa ctatcagatt ttatttttgt ataatttaag
     5101 gaaggtaaag ttaggggact agaagactct aaattggctt ctacagatca ataatttaaa
     5161 tgtaactagt tgggatttta tagttaaaat tatatttgtg tatataacat aactaatctg
     5221 taaattgtaa taaatatatt tgcaattatt aaatgttaa
//
