LOCUS       NM_014330               2350 bp    mRNA    linear   PRI 13-MAY-2024
DEFINITION  Homo sapiens protein phosphatase 1 regulatory subunit 15A
            (PPP1R15A), mRNA.
ACCESSION   NM_014330
VERSION     NM_014330.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Magg,V., Manetto,A., Kopp,K., Wu,C.C., Naghizadeh,M., Lindner,D.,
            Eke,L., Welsch,J., Kallenberger,S.M., Schott,J., Haucke,V.,
            Locker,N., Stoecklin,G. and Ruggieri,A.
  TITLE     Turnover of PPP1R15A mRNA encoding GADD34 controls responsiveness
            and adaptation to cellular stress
  JOURNAL   Cell Rep 43 (4), 114069 (2024)
   PUBMED   38602876
  REMARK    GeneRIF: Turnover of PPP1R15A mRNA encoding GADD34 controls
            responsiveness and adaptation to cellular stress.
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Liu,C., Chen,L., Cong,Y., Cheng,L., Shuai,Y., Lv,F., Chen,K.,
            Song,Y. and Xing,Y.
  TITLE     Protein phosphatase 1 regulatory subunit 15 A promotes translation
            initiation and induces G2M phase arrest during cuproptosis in
            cancers
  JOURNAL   Cell Death Dis 15 (2), 149 (2024)
   PUBMED   38365764
  REMARK    GeneRIF: Protein phosphatase 1 regulatory subunit 15 A promotes
            translation initiation and induces G2M phase arrest during
            cuproptosis in cancers.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2350)
  AUTHORS   Hicks,D., Giresh,K., Wrischnik,L.A. and Weiser,D.C.
  TITLE     The PPP1R15 Family of eIF2-alpha Phosphatase Targeting Subunits
            (GADD34 and CReP)
  JOURNAL   Int J Mol Sci 24 (24), 17321 (2023)
   PUBMED   38139150
  REMARK    GeneRIF: The PPP1R15 Family of eIF2-alpha Phosphatase Targeting
            Subunits (GADD34 and CReP).
            Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2350)
  AUTHORS   Li,H., Li,W., Zhang,S., Qiu,M., Li,Z., Lin,Y., Tan,J. and Qiao,W.
  TITLE     Enterovirus 71 Activates GADD34 via Precursor 3CD to Promote
            IRES-Mediated Viral Translation
  JOURNAL   Microbiol Spectr 10 (1), e0138821 (2022)
   PUBMED   34985336
  REMARK    GeneRIF: Enterovirus 71 Activates GADD34 via Precursor 3CD to
            Promote IRES-Mediated Viral Translation.
REFERENCE   5  (bases 1 to 2350)
  AUTHORS   Hodgson,G., Andreeva,A. and Bertolotti,A.
  TITLE     Substrate recognition determinants of human eIF2alpha phosphatases
  JOURNAL   Open Biol 11 (12), 210205 (2021)
   PUBMED   34847777
  REMARK    GeneRIF: Substrate recognition determinants of human eIF2alpha
            phosphatases.
REFERENCE   6  (bases 1 to 2350)
  AUTHORS   Lee,Y.Y., Cevallos,R.C. and Jan,E.
  TITLE     An upstream open reading frame regulates translation of GADD34
            during cellular stresses that induce eIF2alpha phosphorylation
  JOURNAL   J Biol Chem 284 (11), 6661-6673 (2009)
   PUBMED   19131336
  REMARK    GeneRIF: GADD34 translation is regulated by a unique 5'UTR uORF
            mechanism to ensure proper GADD34 expression during eIF2alpha
            phosphorylation
REFERENCE   7  (bases 1 to 2350)
  AUTHORS   Adler,H.T., Chinery,R., Wu,D.Y., Kussick,S.J., Payne,J.M.,
            Fornace,A.J. Jr. and Tkachuk,D.C.
  TITLE     Leukemic HRX fusion proteins inhibit GADD34-induced apoptosis and
            associate with the GADD34 and hSNF5/INI1 proteins
  JOURNAL   Mol Cell Biol 19 (10), 7050-7060 (1999)
   PUBMED   10490642
REFERENCE   8  (bases 1 to 2350)
  AUTHORS   Hasegawa,T. and Isobe,K.
  TITLE     Evidence for the interaction between Translin and GADD34 in
            mammalian cells
  JOURNAL   Biochim Biophys Acta 1428 (2-3), 161-168 (1999)
   PUBMED   10434033
REFERENCE   9  (bases 1 to 2350)
  AUTHORS   Korabiowska,M., Betke,H., Kellner,S., Stachura,J. and Schauer,A.
  TITLE     Differential expression of growth arrest, DNA damage genes and
            tumour suppressor gene p53 in naevi and malignant melanomas
  JOURNAL   Anticancer Res 17 (5A), 3697-3700 (1997)
   PUBMED   9413226
REFERENCE   10 (bases 1 to 2350)
  AUTHORS   Hollander,M.C., Zhan,Q., Bae,I. and Fornace,A.J. Jr.
  TITLE     Mammalian GADD34, an apoptosis- and DNA damage-inducible gene
  JOURNAL   J Biol Chem 272 (21), 13731-13737 (1997)
   PUBMED   9153226
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC026803.7.
            
            On Nov 19, 2020 this sequence version replaced NM_014330.4.
            
            Summary: This gene is a member of a group of genes whose transcript
            levels are increased following stressful growth arrest conditions
            and treatment with DNA-damaging agents. The induction of this gene
            by ionizing radiation occurs in certain cell lines regardless of
            p53 status, and its protein response is correlated with apoptosis
            following ionizing radiation. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK225632.1, BC003067.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000200453.6/ ENSP00000200453.4
            RefSeq Select criteria :: based on single protein-coding transcript
            regulatory uORF        :: PMID: 19131336
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-231               AC026803.7         109755-109985
            232-1905            AC026803.7         110559-112232
            1906-2350           AC026803.7         112948-113392
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..2350
                     /gene="PPP1R15A"
                     /gene_synonym="GADD34"
                     /note="protein phosphatase 1 regulatory subunit 15A"
                     /db_xref="GeneID:23645"
                     /db_xref="HGNC:HGNC:14375"
                     /db_xref="MIM:611048"
     exon            1..231
                     /gene="PPP1R15A"
                     /gene_synonym="GADD34"
                     /inference="alignment:Splign:2.1.0"
     regulatory      134..214
                     /regulatory_class="uORF"
                     /gene="PPP1R15A"
                     /gene_synonym="GADD34"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:19131336]"
                     /note="this uORF is predicted to encode a 26 aa peptide
                     (PMID:19131336)"
     exon            232..1905
                     /gene="PPP1R15A"
                     /gene_synonym="GADD34"
                     /inference="alignment:Splign:2.1.0"
     CDS             241..2265
                     /gene="PPP1R15A"
                     /gene_synonym="GADD34"
                     /note="growth arrest and DNA-damage-inducible 34; growth
                     arrest and DNA damage-inducible protein GADD34; myeloid
                     differentiation primary response protein MyD116 homolog;
                     protein phosphatase 1, regulatory (inhibitor) subunit 15A"
                     /codon_start=1
                     /product="protein phosphatase 1 regulatory subunit 15A"
                     /protein_id="NP_055145.3"
                     /db_xref="CCDS:CCDS12738.1"
                     /db_xref="GeneID:23645"
                     /db_xref="HGNC:HGNC:14375"
                     /db_xref="MIM:611048"
                     /translation="MAPGQAPHQATPWRDAHPFFLLSPVMGLLSRAWSRLRGLGPLEP
                     WLVEAVKGAALVEAGLEGEARTPLAIPHTPWGRRPEEEAEDSGGPGEDRETLGLKTSS
                     SLPEAWGLLDDDDGMYGEREATSVPRGQGSQFADGQRAPLSPSLLIRTLQGSDKNPGE
                     EKAEEEGVAEEEGVNKFSYPPSHRECCPAVEEEDDEEAVKKEAHRTSTSALSPGSKPS
                     TWVSCPGEEENQATEDKRTERSKGARKTSVSPRSSGSDPRSWEYRSGEASEEKEEKAH
                     KETGKGEAAPGPQSSAPAQRPQLKSWWCQPSDEEEGEVKALGAAEKDGEAECPPCIPP
                     PSAFLKAWVYWPGEDTEEEEDEEEDEDSDSGSDEEEGEAEASSSTPATGVFLKSWVYQ
                     PGEDTEEEEDEDSDTGSAEDEREAETSASTPPASAFLKAWVYRPGEDTEEEEDEDVDS
                     EDKEDDSEAALGEAESDPHPSHPDQRAHFRGWGYRPGKETEEEEAAEDWGEAEPCPFR
                     VAIYVPGEKPPPPWAPPRLPLRLQRRLKRPETPTHDPDPETPLKARKVRFSEKVTVHF
                     LAVWAGPAQAARQGPWEQLARDRSRFARRITQAQEELSPCLTPAARARAWARLRNPPL
                     APIPALTQTLPSSSVPSSPVQTTPLSQAVATPSRSSAAAAAALDLSGRRG"
     exon            1906..2350
                     /gene="PPP1R15A"
                     /gene_synonym="GADD34"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2330..2335
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP1R15A"
                     /gene_synonym="GADD34"
                     /note="hexamer: AATAAA"
     polyA_site      2350
                     /gene="PPP1R15A"
                     /gene_synonym="GADD34"
                     /note="major polyA site"
ORIGIN      
        1 gctcttatcg gttcccatcc cagttgttga tcttatgcaa gacgctgcac gaccccgcgc
       61 ccgcttgtcg ccacggcact tgaggcagcc ggagatactc tgagttactc ggagcccgac
      121 gcctgagggt gagatgaacg cgctggcctc cctaaccgtc cggacctgtg atcgcttctg
      181 gcagaccgaa ccggcgctcc tgcccccggg gtgacgcgca gctcccagcc gcccagacac
      241 atggccccag gccaagcacc ccatcaggct accccgtgga gggatgccca ccctttcttc
      301 ctcctgtccc cagtgatggg cctcctcagc cgcgcctgga gccgcctgag gggcctggga
      361 cctctagagc cctggctggt ggaagcagta aaaggagcag ctctggtaga agctggcctg
      421 gagggagaag ctaggactcc tctggcaatc ccccataccc cttggggcag acgccctgaa
      481 gaggaggctg aagacagtgg aggccctgga gaggacagag aaacactggg gctgaaaacc
      541 agcagttccc ttcctgaagc ctggggactt ttggatgatg atgatggcat gtatggtgag
      601 cgagaggcaa ccagtgtccc tagagggcag ggaagtcaat ttgcagatgg ccagcgtgct
      661 cccctgtctc ccagccttct gataaggaca ctgcaaggtt ctgataagaa cccaggggag
      721 gagaaagccg aggaagaggg agttgctgaa gaggagggag ttaacaagtt ctcttatcca
      781 ccatcacacc gggagtgttg tccagccgtg gaggaggagg acgatgaaga agctgtaaag
      841 aaagaagctc acagaacctc tacttctgcc ttgtctccag gatccaagcc cagcacttgg
      901 gtgtcttgcc caggggagga agagaatcaa gccacggagg ataaaagaac agaaagaagt
      961 aaaggagcca ggaagacctc cgtgtccccc cgatcttcag gctccgaccc caggtcctgg
     1021 gagtatcgtt caggagaggc gtccgaggag aaggaggaaa aggcacacaa agaaactggg
     1081 aaaggagaag ctgccccagg gccgcaatcc tcagccccag cccagaggcc ccagctcaag
     1141 tcctggtggt gccaacccag tgatgaagag gagggtgagg tcaaggcttt gggggcagct
     1201 gagaaggatg gagaagctga gtgtcctccc tgcatccccc caccaagtgc cttcctgaag
     1261 gcctgggtgt attggccagg agaggacaca gaggaagagg aagatgagga agaagatgag
     1321 gacagtgact ctggatcaga tgaggaagag ggagaagctg aggcttcctc ttccactcct
     1381 gctacaggtg tcttcttgaa gtcctgggtc tatcagccag gagaggacac agaggaggag
     1441 gaagatgagg acagtgatac aggatcagcc gaggatgaaa gagaagctga gacttctgct
     1501 tccacacccc ctgcaagtgc tttcttgaag gcctgggtgt atcggccagg agaggacacg
     1561 gaggaggagg aagatgagga tgtggatagt gaggataagg aagatgattc agaagcagcc
     1621 ttgggagaag ctgagtcaga cccacatccc tcccacccgg accagagggc ccacttcagg
     1681 ggctggggat atcgacctgg aaaagagaca gaggaagagg aagctgctga ggactgggga
     1741 gaagctgagc cctgcccctt ccgagtggcc atctatgtac ctggagagaa gccaccgcct
     1801 ccctgggctc ctcctaggct gcccctccga ctgcaaaggc ggctcaagcg cccagaaacc
     1861 cctactcatg atccggaccc tgagactccc ctaaaggcca gaaaggtgcg cttctccgag
     1921 aaggtcactg tccatttcct ggctgtctgg gcagggccgg cccaggccgc ccgccagggc
     1981 ccctgggagc agcttgctcg ggatcgcagc cgcttcgcac gccgcatcac ccaggcccag
     2041 gaggagctga gcccctgcct cacccctgct gcccgggcca gagcctgggc acgcctcagg
     2101 aacccacctt tagcccccat ccctgccctc acccagacct tgccttcctc ctctgtccct
     2161 tcgtccccag tccagaccac gcccttgagc caagctgtgg ccacaccttc ccgctcgtct
     2221 gctgctgcag cggctgccct ggacctcagt gggaggcgtg gctgagacca actggtttgc
     2281 ctataattta ttaactattt attttttcta agtgtgggtt tatataagga ataaagcctt
     2341 ttgatttgta
//
