LOCUS       NM_033256                560 bp    mRNA    linear   PRI 06-AUG-2024
DEFINITION  Homo sapiens protein phosphatase 1 regulatory inhibitor subunit 14A
            (PPP1R14A), transcript variant 1, mRNA.
ACCESSION   NM_033256
VERSION     NM_033256.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Ma,J.J., Zhang,L., Lu,J. and Zhang,H.X.
  TITLE     PPP1R14A is Associated with Immunotherapy Resistance in Head and
            Neck Squamous Cell Carcinoma Identified by Single-Cell and Bulk
            RNA-Sequencing
  JOURNAL   Chin Med Sci J 39 (2), 111-121 (2024)
   PUBMED   38887993
  REMARK    GeneRIF: PPP1R14A is Associated with Immunotherapy Resistance in
            Head and Neck Squamous Cell Carcinoma Identified by Single-Cell and
            Bulk RNA-Sequencing.
REFERENCE   2  (bases 1 to 560)
  AUTHORS   Dong,Y., Wang,J., Yang,C., Bao,J., Liu,X., Chen,H., Zhang,X.,
            Shi,W., Zhang,L., Qi,Q., Li,Y., Wang,S., Ma,R., Cong,B. and
            Zhang,G.
  TITLE     Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary Artery
            Spasm-Induced Sudden Cardiac Death
  JOURNAL   Int J Mol Sci 25 (5), 2941 (2024)
   PUBMED   38474189
  REMARK    GeneRIF: Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary
            Artery Spasm-Induced Sudden Cardiac Death.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 560)
  AUTHORS   Tian,Y., Soupir,A., Liu,Q., Wu,L., Huang,C.C., Park,J.Y. and
            Wang,L.
  TITLE     Novel role of prostate cancer risk variant rs7247241 on PPP1R14A
            isoform transition through allelic TF binding and CpG methylation
  JOURNAL   Hum Mol Genet 31 (10), 1610-1621 (2022)
   PUBMED   34849858
  REMARK    GeneRIF: Novel role of prostate cancer risk variant rs7247241 on
            PPP1R14A isoform transition through allelic TF binding and CpG
            methylation.
REFERENCE   4  (bases 1 to 560)
  AUTHORS   Xu,J., Zhang,Y., Shi,Y., Yin,D., Dai,P., Zhao,W. and Zhang,T.
  TITLE     CPI-17 Overexpression and Its Correlation With the NF2 Mutation
            Spectrum in Sporadic Vestibular Schwannomas
  JOURNAL   Otol Neurotol 41 (1), e94-e102 (2020)
   PUBMED   31789805
  REMARK    GeneRIF: CPI-17 Overexpression and Its Correlation With the NF2
            Mutation Spectrum in Sporadic Vestibular Schwannomas.
REFERENCE   5  (bases 1 to 560)
  AUTHORS   Liao,Q., Yan,J., Zhou,Z., Luo,J., Han,Q., Zhang,Q. and Chen,R.
  TITLE     Relationship between uterine smooth muscular CPI-17-signal
            pathway-mediated Ca2+ sensitivity changes and uterine atony-induced
            postpartum haemorrhage
  JOURNAL   J Obstet Gynaecol 39 (3), 302-307 (2019)
   PUBMED   30428734
  REMARK    GeneRIF: The down-regulated expression of CPI-17 phosphorylated
            proteins might be one of the important factors of uterine
            atony-induced postpartum hemorrhage.
REFERENCE   6  (bases 1 to 560)
  AUTHORS   Yamawaki,K., Ito,M., Machida,H., Moriki,N., Okamoto,R., Isaka,N.,
            Shimpo,H., Kohda,A., Okumura,K., Hartshorne,D.J. and Nakano,T.
  TITLE     Identification of human CPI-17, an inhibitory phosphoprotein for
            myosin phosphatase
  JOURNAL   Biochem Biophys Res Commun 285 (4), 1040-1045 (2001)
   PUBMED   11467857
REFERENCE   7  (bases 1 to 560)
  AUTHORS   Hamaguchi,T., Ito,M., Feng,J., Seko,T., Koyama,M., Machida,H.,
            Takase,K., Amano,M., Kaibuchi,K., Hartshorne,D.J. and Nakano,T.
  TITLE     Phosphorylation of CPI-17, an inhibitor of myosin phosphatase, by
            protein kinase N
  JOURNAL   Biochem Biophys Res Commun 274 (3), 825-830 (2000)
   PUBMED   10924361
REFERENCE   8  (bases 1 to 560)
  AUTHORS   Koyama,M., Ito,M., Feng,J., Seko,T., Shiraki,K., Takase,K.,
            Hartshorne,D.J. and Nakano,T.
  TITLE     Phosphorylation of CPI-17, an inhibitory phosphoprotein of smooth
            muscle myosin phosphatase, by Rho-kinase
  JOURNAL   FEBS Lett 475 (3), 197-200 (2000)
   PUBMED   10869555
REFERENCE   9  (bases 1 to 560)
  AUTHORS   Eto,M., Senba,S., Morita,F. and Yazawa,M.
  TITLE     Molecular cloning of a novel phosphorylation-dependent inhibitory
            protein of protein phosphatase-1 (CPI17) in smooth muscle: its
            specific localization in smooth muscle
  JOURNAL   FEBS Lett 410 (2-3), 356-360 (1997)
   PUBMED   9237662
REFERENCE   10 (bases 1 to 560)
  AUTHORS   Eto,M., Ohmori,T., Suzuki,M., Furuya,K. and Morita,F.
  TITLE     A novel protein phosphatase-1 inhibitory protein potentiated by
            protein kinase C. Isolation from porcine aorta media and
            characterization
  JOURNAL   J Biochem 118 (6), 1104-1107 (1995)
   PUBMED   8720121
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB056508.1.
            
            On Nov 22, 2018 this sequence version replaced NM_033256.2.
            
            Summary: The protein encoded by this gene belongs to the protein
            phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor
            of smooth muscle myosin phosphatase, and has higher inhibitory
            activity when phosphorylated. Inhibition of myosin phosphatase
            leads to increased myosin phosphorylation and enhanced smooth
            muscle contraction. Alternatively spliced transcript variants
            encoding different isoforms have been noted for this gene.
            [provided by RefSeq, Sep 2011].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript, and encodes the longer isoform (1, also known as
            CPI-17alpha).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB056508.1, AY050670.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000301242.9/ ENSP00000301242.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-560               AB056508.1         159-718
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.2"
     gene            1..560
                     /gene="PPP1R14A"
                     /gene_synonym="CPI-17; CPI17; PPP1INL"
                     /note="protein phosphatase 1 regulatory inhibitor subunit
                     14A"
                     /db_xref="GeneID:94274"
                     /db_xref="HGNC:HGNC:14871"
                     /db_xref="MIM:608153"
     exon            1..236
                     /gene="PPP1R14A"
                     /gene_synonym="CPI-17; CPI17; PPP1INL"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..479
                     /gene="PPP1R14A"
                     /gene_synonym="CPI-17; CPI17; PPP1INL"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein phosphatase 1 regulatory subunit 14A;
                     PKC-potentiated inhibitory protein of PP1; 17 kDa
                     PKC-potentiated inhibitory protein of PP1; protein kinase
                     C-potentiated inhibitor protein of 17 kDa; 17-KDa protein"
                     /codon_start=1
                     /product="protein phosphatase 1 regulatory subunit 14A
                     isoform 1"
                     /protein_id="NP_150281.1"
                     /db_xref="CCDS:CCDS12509.1"
                     /db_xref="GeneID:94274"
                     /db_xref="HGNC:HGNC:14871"
                     /db_xref="MIM:608153"
                     /translation="MAAQRLGKRVLSKLQSPSRARGPGGSPGGLQKRHARVTVKYDRR
                     ELQRRLDVEKWIDGRLEELYRGMEADMPDEINIDELLELESEEERSRKIQGLLKSCGK
                     PVEDFIQELLAKLQGLHRQPGLRQPSPSHDGSLSPLQDRARTAHP"
     exon            237..317
                     /gene="PPP1R14A"
                     /gene_synonym="CPI-17; CPI17; PPP1INL"
                     /inference="alignment:Splign:2.1.0"
     exon            318..350
                     /gene="PPP1R14A"
                     /gene_synonym="CPI-17; CPI17; PPP1INL"
                     /inference="alignment:Splign:2.1.0"
     exon            351..560
                     /gene="PPP1R14A"
                     /gene_synonym="CPI-17; CPI17; PPP1INL"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      560
                     /gene="PPP1R14A"
                     /gene_synonym="CPI-17; CPI17; PPP1INL"
                     /note="major polyA site"
ORIGIN      
        1 gcacagcgcg gggccaggct gggtccagca gcgcgatggc agctcagcgg ctgggcaagc
       61 gcgtgctgag caagctgcag tctccatcgc gggcccgcgg gccagggggc agtcccgggg
      121 ggctgcagaa gcggcacgcg cgcgtcaccg tcaagtatga ccggcgggag ctgcagcggc
      181 ggctggacgt ggagaagtgg atcgacgggc gcctggagga gctgtaccgc ggcatggagg
      241 cagacatgcc cgatgagatc aacattgatg aattgttgga gttagagagt gaagaggaga
      301 gaagccggaa aatccaggga ctcctgaagt catgtgggaa acctgtcgag gacttcatcc
      361 aggagctgct ggcaaagctt caaggcctcc acaggcagcc cggcctccgc cagccaagcc
      421 cctcccacga cggcagcctc agccccctcc aggaccgggc ccggactgct cacccctgac
      481 cctcttgcac tctccctgcc ccccggacgc cgcccagctt gcttgtgtat aagttgtatt
      541 taatggttct gtaacaataa
//
