LOCUS       NM_002481              15244 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens protein phosphatase 1 regulatory subunit 12B
            (PPP1R12B), transcript variant 1, mRNA.
ACCESSION   NM_002481
VERSION     NM_002481.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 15244)
  AUTHORS   Jia,D., Wang,K., Huang,L., Zhou,Z., Zhang,Y., Chen,N., Yang,Q.,
            Wen,Z., Jiang,H., Yao,C. and Wu,R.
  TITLE     Revealing PPP1R12B and COL1A1 as piRNA pathway genes contributing
            to abdominal aortic aneurysm through integrated analysis and
            experimental validation
  JOURNAL   Gene 897, 148068 (2024)
   PUBMED   38070790
  REMARK    GeneRIF: Revealing PPP1R12B and COL1A1 as piRNA pathway genes
            contributing to abdominal aortic aneurysm through integrated
            analysis and experimental validation.
REFERENCE   2  (bases 1 to 15244)
  AUTHORS   Song,S.E., Kim,Y., Jeong,H., Lee,B., Lee,J., Roh,J.S., So,M.W.,
            Lee,S.G. and Sohn,D.H.
  TITLE     FAT10 differentially stabilizes MYPT2 isoforms
  JOURNAL   Biochem Biophys Res Commun 676, 115-120 (2023)
   PUBMED   37506472
  REMARK    GeneRIF: FAT10 differentially stabilizes MYPT2 isoforms.
REFERENCE   3  (bases 1 to 15244)
  AUTHORS   Tan,J., Lu,T., Xu,J., Hou,Y., Chen,Z., Zhou,K., Ding,Y., Jiang,B.
            and Zhu,Y.
  TITLE     MicroRNA-4463 facilitates the development of colon cancer by
            suppression of the expression of PPP1R12B
  JOURNAL   Clin Transl Oncol 24 (6), 1115-1123 (2022)
   PUBMED   35064454
  REMARK    GeneRIF: MicroRNA-4463 facilitates the development of colon cancer
            by suppression of the expression of PPP1R12B.
REFERENCE   4  (bases 1 to 15244)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 15244)
  AUTHORS   Bannert,N., Vollhardt,K., Asomuddinov,B., Haag,M., Konig,H.,
            Norley,S. and Kurth,R.
  TITLE     PDZ Domain-mediated interaction of interleukin-16 precursor
            proteins with myosin phosphatase targeting subunits
  JOURNAL   J Biol Chem 278 (43), 42190-42199 (2003)
   PUBMED   12923170
REFERENCE   6  (bases 1 to 15244)
  AUTHORS   Arimura,T., Suematsu,N., Zhou,Y.B., Nishimura,J., Satoh,S.,
            Takeshita,A., Kanaide,H. and Kimura,A.
  TITLE     Identification, characterization, and functional analysis of
            heart-specific myosin light chain phosphatase small subunit
  JOURNAL   J Biol Chem 276 (9), 6073-6082 (2001)
   PUBMED   11067852
REFERENCE   7  (bases 1 to 15244)
  AUTHORS   Somlyo,A.P.
  TITLE     Kinases, myosin phosphatase and Rho proteins: curiouser and
            curiouser
  JOURNAL   J Physiol 516 (Pt 3) (Pt 3), 630 (1999)
   PUBMED   10200412
REFERENCE   8  (bases 1 to 15244)
  AUTHORS   Somlyo,A.P., Wu,X., Walker,L.A. and Somlyo,A.V.
  TITLE     Pharmacomechanical coupling: the role of calcium, G-proteins,
            kinases and phosphatases
  JOURNAL   Rev Physiol Biochem Pharmacol 134, 201-234 (1999)
   PUBMED   10087910
  REMARK    Review article
REFERENCE   9  (bases 1 to 15244)
  AUTHORS   Moorhead,G., Johnson,D., Morrice,N. and Cohen,P.
  TITLE     The major myosin phosphatase in skeletal muscle is a complex
            between the beta-isoform of protein phosphatase 1 and the MYPT2
            gene product
  JOURNAL   FEBS Lett 438 (3), 141-144 (1998)
   PUBMED   9827534
REFERENCE   10 (bases 1 to 15244)
  AUTHORS   Fujioka,M., Takahashi,N., Odai,H., Araki,S., Ichikawa,K., Feng,J.,
            Nakamura,M., Kaibuchi,K., Hartshorne,D.J., Nakano,T. and Ito,M.
  TITLE     A new isoform of human myosin phosphatase targeting/regulatory
            subunit (MYPT2): cDNA cloning, tissue expression, and chromosomal
            mapping
  JOURNAL   Genomics 49 (1), 59-68 (1998)
   PUBMED   9570949
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL356953.17, AK307591.1,
            BC144699.1, AF324888.1, AC099336.2 and AC118556.2.
            
            On Nov 22, 2018 this sequence version replaced NM_002481.3.
            
            Summary: Myosin phosphatase is a protein complex comprised of three
            subunits: a catalytic subunit (PP1c-delta, protein phosphatase 1,
            catalytic subunit delta), a large regulatory subunit (MYPT, myosin
            phosphatase target) and small regulatory subunit (sm-M20). Two
            isoforms of MYPT have been isolated--MYPT1 and MYPT2, the first of
            which is widely expressed, and the second of which may be specific
            to heart, skeletal muscle, and brain. Each of the MYPT isoforms
            functions to bind PP1c-delta and increase phosphatase activity.
            This locus encodes both MYTP2 and M20. Alternatively spliced
            transcript variants encoding different isoforms have been
            identified. Related pseudogenes have been defined on the Y
            chromosome. [provided by RefSeq, Oct 2011].
            
            Transcript Variant: This variant (1) lacks an alternate in-frame
            exon compared to variant 8. The resulting isoform (a, also known as
            the large myosin binding subunit) has the same N- and C-termini but
            is shorter compared to isoform h.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.139958.1, AF324888.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000608999.6/ ENSP00000476755.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AL356953.17        165718-165720
            4-11                AK307591.1         1-8
            12-5224             BC144699.1         1-5213
            5225-11111          AF324888.1         5216-11102
            11112-12794         AC099336.2         158156-159838
            12795-15244         AC118556.2         6576-9025           c
FEATURES             Location/Qualifiers
     source          1..15244
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.1"
     gene            1..15244
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /note="protein phosphatase 1 regulatory subunit 12B"
                     /db_xref="GeneID:4660"
                     /db_xref="HGNC:HGNC:7619"
                     /db_xref="MIM:603768"
     exon            1..444
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     CDS             154..3102
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /EC_number="3.1.3.53"
                     /note="isoform a is encoded by transcript variant 1;
                     myosin phosphatase target subunit 2; myosin
                     phosphatase-targeting subunit 2; protein phosphatase 1,
                     regulatory (inhibitor) subunit 12B"
                     /codon_start=1
                     /product="protein phosphatase 1 regulatory subunit 12B
                     isoform a"
                     /protein_id="NP_002472.2"
                     /db_xref="CCDS:CCDS1426.1"
                     /db_xref="GeneID:4660"
                     /db_xref="HGNC:HGNC:7619"
                     /db_xref="MIM:603768"
                     /translation="MAELEHLGGKRAESARMRRAEQLRRWRGSLTEQEPAERRGAGRQ
                     PLTRRGSPRVRFEDGAVFLAACSSGDTDEVRKLLARGADINTVNVDGLTALHQACIDE
                     NLDMVKFLVENRANVNQQDNEGWTPLHAAASCGYLNIAEYFINHGASVGIVNSEGEVP
                     SDLAEEPAMKDLLLEQVKKQGVDLEQSRKEEEQQMLQDARQWLNSGKIEDVRQARSGA
                     TALHVAAAKGYSEVLRLLIQAGYELNVQDYDGWTPLHAAAHWGVKEACSILAEALCDM
                     DIRNKLGQTPFDVADEGLVEHLELLQKKQNVLRSEKETRNKLIESDLNSKIQSGFFKN
                     KEKMLYEEETPKSQEMEEENKESSSSSSEEEEGEDEASESETEKEADKKPEAFVNHSN
                     SESKSSITEQIPAPAQNTFSASSARRFSSGLFNKPEEPKDESPSSWRLGLRKTGSHNM
                     LSEVANSREPIRDRGSSIYRSSSSPRISALLDNKDKERENKSYISSLAPRKLNSTSDI
                     EEKENRESAVNLVRSGSYTRQLWRDEAKGNEIPQTIAPSTYVSTYLKRTPHKSQADTT
                     AEKTADNVSSSTPLCVITNRPLPSTANGVTATPVLSITGTDSSVEAREKRRSYLTPVR
                     DEEAESLRKARSRQARQTRRSTQGVTLTDLQEAERTFSRSRAERQAQEQPREKPTDTE
                     GLEGSPEKHEPSAVPATEAGEGQQPWGRSLDEEPICHRLRCPAQPDKPTTPASPSTSR
                     PSLYTSSHLLWTNRFSVPDSESSETTTNTTTAKEMDKNENEEADLDEQSSKRLSIRER
                     RRPKERRRGTGINFWTKDEDETDGSEEVKETWHERLSRLESGGSNPTTSDSYGDRASA
                     RARREAREARLATLTSRVEEDSNRDYKKLYESALTENQKLKTKLQEAQLELADIKSKL
                     EKVAQQKQEKTSDRSSVLEMEKRERRALERKMSEMEEEMKVLTELKSDNQRLKDENGA
                     LIRVISKLSK"
     exon            445..575
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            576..694
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            695..854
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            855..999
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1074
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            1075..1154
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1294
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            1295..1407
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1611
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            1612..1694
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1820
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..2003
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2094
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            2095..2298
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            2299..2488
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            2489..2601
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            2602..2643
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            2644..2660
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            2661..2805
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            2806..2910
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            2911..2964
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            2965..3015
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     exon            3016..15244
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11089..11094
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /note="hexamer: AATAAA"
     polyA_site      11111
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /note="major polyA site"
     regulatory      15220..15225
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
                     /note="hexamer: AATAAA"
     polyA_site      15244
                     /gene="PPP1R12B"
                     /gene_synonym="MYPT2; PP1bp55"
ORIGIN      
        1 ggtactactg gcgggaggag taaagatggc ggcgcgaggg tctccgccct ctgctccggg
       61 ctgaagcgct ctgagagagg cggcagcggc aactcgagcc ccaacagtaa tttagtgttg
      121 gtagttttgg cagcagctgc cgaggccgga gcaatggcgg aactggagca cctaggaggg
      181 aagcgggcag agtcggcgcg aatgcggcgg gcagagcagc ttcggcgctg gcggggctcg
      241 ctgacagagc aggagcctgc ggagcgacga ggcgcggggc ggcagccgct gaccaggcgc
      301 gggagcccca gggtccgctt cgaggacggt gctgtctttc tggccgcctg ctctagcggg
      361 gacaccgacg aggtgagaaa gcttctggca agaggtgctg atatcaacac ggtcaacgtg
      421 gacggcttga cagccctgca ccaggcatgt attgatgaaa atttggacat ggtgaagttt
      481 ctggtggaga acagagccaa tgtaaaccag caagacaacg agggctggac accccttcat
      541 gcagcagctt cctgtggcta tctcaacata gcagagtatt tcattaatca cggagccagt
      601 gtaggtattg tcaatagtga aggtgaagtt ccctctgacc ttgcagaaga gccagccatg
      661 aaggatcttc ttctggagca agtaaagaag caaggagttg atctagagca gtcaagaaaa
      721 gaagaagagc agcagatgtt gcaggatgcc cgccagtggc tcaacagtgg gaaaatagag
      781 gatgtgaggc aggctcgctc aggggctaca gcccttcatg tggctgctgc caagggctac
      841 tctgaagtcc tcagactttt aattcaggct ggctatgaac tcaatgttca ggattatgat
      901 ggctggactc ccctccatgc tgctgcacac tggggagtga aggaggcttg ctccatcctg
      961 gcagaagcac tttgtgacat ggatattcga aataaactgg gccagacacc atttgatgtg
     1021 gctgatgagg gtctcgtgga gcatttggag ttgctccaga agaagcagaa tgtgcttcga
     1081 agtgaaaagg agacacggaa taaactcatt gagtcagatc tgaacagcaa gattcagagt
     1141 gggttcttta agaacaaaga gaagatgctc tatgaggagg agacacctaa gtcccaagaa
     1201 atggaggaag aaaataaaga atctagtagc tccagctcag aggaggagga aggtgaagat
     1261 gaagcttctg agtcagaaac tgagaaggag gcagataaaa agccagaagc ctttgtcaat
     1321 cattccaact ctgaaagcaa gagtagtatc acagagcaga taccagcacc agctcaaaat
     1381 accttctctg cctcttctgc taggaggttc tcttctggcc tttttaacaa gccagaagag
     1441 cccaaagatg aatctccttc ttcatggaga ttgggactga gaaaaactgg cagccacaac
     1501 atgctgagtg aggtggccaa ttccagggaa cctataaggg accgaggctc ttccatctat
     1561 cgctcctctt caagccctcg gatttctgct ctactggaca acaaagataa ggagagagaa
     1621 aacaaaagct atattagttc actagcaccc cggaagctca acagcacaag tgatattgaa
     1681 gaaaaggaga acagagaatc agctgttaat ctagtgagga gtggctccta tacccggcag
     1741 ctatggaggg atgaagcaaa aggaaatgaa atcccacaga caattgctcc ctccacctat
     1801 gtatcaactt acttgaaaag gactcctcac aaatcccagg ccgacacaac agcagagaaa
     1861 acagcagaca atgtctcttc tagcaccccg ctctgtgtga tcaccaatcg ccctcttcct
     1921 agcactgcca atggggttac agctactcct gtgctctcca ttactggaac agattcctct
     1981 gtggaagcca gggagaagag gaggtcctat ctgactcctg tacgggatga ggaagcagag
     2041 tctttacgga aagcacgctc cagacaagct cggcagacac gaaggtctac tcaaggtgtc
     2101 accctaacag accttcaaga agcagaaagg acattcagcc ggtcgagggc agagaggcaa
     2161 gctcaggagc agcctcgtga gaagcccaca gacactgaag ggcttgaggg gagccctgag
     2221 aagcatgagc cctcagcagt tccagcaaca gaagctgggg agggccagca gccctggggc
     2281 aggagtctgg atgaagagcc tatctgtcat cgcctgaggt gcccagctca gccagacaaa
     2341 cccaccacgc cagcatctcc ttctacgtca agaccctcac tctacaccag ttcccacctg
     2401 ctatggacaa atagattttc agtccctgat tctgagagtt cagagactac cacaaacact
     2461 acaactgcaa aggaaatgga caaaaatgag aatgaagaag cagatttgga tgagcagtcc
     2521 tctaagaggc tgtccatccg agagaggagg cggcccaagg aacgacgaag aggcacaggc
     2581 atcaatttct ggacaaagga tgaggatgaa actgatggct ctgaagaggt caaagaaacg
     2641 tggcatgaaa gactttctag gttggaatcg ggaggtagta atcctacaac cagtgattct
     2701 tacggtgacc gggcttcagc aagagcccgt cgggaggccc gggaggcccg cctagccacc
     2761 ctgaccagcc gtgtagaaga agacagcaac agagattata aaaaactcta tgagagtgct
     2821 ctgactgaaa accaaaaact gaaaacaaaa cttcaggaag cccagctaga gctagcagat
     2881 ataaagtcca agcttgagaa ggtggcccag cagaaacaag aaaagacctc tgaccgatca
     2941 tcagtgctgg agatggagaa acgggagagg cgagccttgg agcgcaaaat gtcagaaatg
     3001 gaggaagaaa tgaaggtgtt aacagaactg aaatccgaca accagaggct gaaagatgaa
     3061 aatggtgccc tcatcagagt catcagcaaa ctgtccaagt aggctaggct ccagatttat
     3121 gaggaaagaa agggacagca tttgctgccc ccacccctct tttccagtcc ttgccttcca
     3181 accaaaagaa atggatgttt tggtggaagg acacttcttt ctatcaccct cttcagtcac
     3241 ctctatacac tctacatttt ctctgcactt tcaatgccct gttcttccaa acccctatcc
     3301 caagttttat gacagtttta attgaagcat gattgtggta attcgagcca tctggagaat
     3361 gctctgggga gtacaccagg ctcagctgtg gacccctcaa cttcctgctg ctcagctact
     3421 ttgtccacat tggatttggt ccaaacatgt aagacttcta ccctaatcag tatccttcag
     3481 ctttttacat taacccagtg tcctctgata taggtgagtc ttgtggtagc cactccagga
     3541 tcctgattgg ggtgccaaga gaaacagcag gatgttgaat tgatcatcag atgccctctg
     3601 gaatggttag catccaaggt gacagtgact gcattgaggc gctctattct tcttcacctc
     3661 tcaggaactg acttttattt tttctgtcaa cacccagtaa tctccctaac tagttttaac
     3721 ccttattcct ccctcatacc tagccatttc tccaaggcgc aaatggccct ggcttcattt
     3781 attcctttcc tttctatcct tttatatttt tcccttcccc accccctcac tcaatggtat
     3841 aaaagctagg acagagcacc tgacctcagt tgtctttggc cattgtggga agtcattatt
     3901 ctggagacaa gaaaatcatc actctggtgc cttggtggca gcaccactgc ctgctccctg
     3961 gaaggtagac tatggcataa gtgtcagctc attgttttcg tctcccactt atcttccccc
     4021 ttcaaagtga ttttctttat aagaaaattt gggctctgaa tactccattc tgcctcactt
     4081 ccttgacctt gttttcctct cttcgccctg acacctgctt ctaatgagaa atgaagtcag
     4141 tgcaaggagg aatgagaggt ggggagggga gattgttgag agatggacta ggaagtggaa
     4201 gcaggctgga aagacacata tggcgaaagt caagtatctg aggccacttg caacattgtg
     4261 acaaagaaga tggaagatag cagcctactg ggctgtgaga atagtggtga atttgattat
     4321 ttccagtggt tagatttagc agagagattt actttcttag aaattaatag gggaaatggc
     4381 acttaataga ggtttaatgg aatgaagtac tttcaaccca ttgatgagag tcatggaagt
     4441 ttagagtggc aagacccaga ggtcattagg tccagctttc tattattgtg tcacagggac
     4501 ccctgctacc acagctttca cccatgggca tggtcaggta gcctctggtg gctatgcatt
     4561 tattactcaa aagttgggca actctaataa cgagagagtt tttcctcata tcagctgaag
     4621 tctgcctgtg gattctcctt gtagtccaga tctactatct gcaactcttt aacggcactt
     4681 gagatatgtg cagtgggtgg tctccccctc agtcttactt tgagctgctg ttactctagt
     4741 cctttaagtc attcctcata tggcatgctt ttcttacctg tcacatcttg gccatcctcc
     4801 tctagacaaa accagccttt aaaatatcat gtccaggcct ggaaagaata caaatccagt
     4861 gcaaaattaa accattagtt cttgatgata acctagcatt aatacttaca gtattttctt
     4921 tttgtaggag ttttatccct tggttcactt gagtgtgcaa gtcattaaat cctcatcatt
     4981 ttagggttgc cagtgatatt cagtgtttag agtggttcat tcccttaata tatactacct
     5041 ctgtctgtta attctttcct aacatgacat gtagctctcc atggtcctcc ctctgaaaca
     5101 ccacatgctg tttaaactag aagctgtaat tcaagatccc aattccacca ggtgcagtgg
     5161 ctcatgcctg taatcccagc acttcaggag gccgaggtgg acagatcacg aggtcaggag
     5221 ttcgagacca tcctggctaa cacggtgaaa cccaatctct actaaaaata caaaaaatta
     5281 gccaggcgtg gtggcaggcg cctgtagtcc cagctactca ggaggctgag gcaggagaat
     5341 ggcgtgaacc cgggaggcgg agcttgcagt gagccttgat cacgccactg cactccagcc
     5401 tgggtgacag agcaagactc catctcaaaa aaaataataa taataaataa ataaaaaatc
     5461 ccaattacag aacaggagat atgtttcctc tgacaaaacc ccatttttag agtgtataaa
     5521 attctgtctt tcatttccaa ccatttttca agagaagctg ctactatctt gagacttcgt
     5581 attatcatgg gagtactgta agttcagttc tcagggaatg ctaaattaga gtaataagtt
     5641 ttatatcctt cattctggga gaggaaatca acataaggtg ttgactaatt gattatcctg
     5701 gaacattttg aatattgggg aaggaagatg atacttcttt ctcaagaaaa gaaaattgga
     5761 ttattaggaa tacatcttga cccttttcca ctcaatagac cagggaaagg ggttacagag
     5821 agcctcctca gatctgtttg tgcctttcaa aagtactggg ggcagattct caaaactctt
     5881 agattggtgt tgcatccctg ccacttcaca tgacccttcc atgttaaagc tgacttttct
     5941 tgccagaaag ttttccttgt gacaagaaat ttgcaagctt ttcaatctca ggaatactgg
     6001 attctatagc caagaactca tgaaagtaag tgtaaactat cctaggtaat ttcaggtact
     6061 ttctcttatg caattaattt tattaactga tttctggtct atctccagag ataaaatacc
     6121 attccttcac aactgtattg tctggaagcc ccatgagatg gtaattatag gccttctact
     6181 agccagcagt tctctctgga tactggcatc agcaggtctc ctttttcttg tgctgcttgg
     6241 acaggtacct actgctggcc tcttcagtgg ccacaatcct cagtcctcac ctgctggttt
     6301 ggagactcat gagcacccca ggcagctcaa gctgaataaa tagctgcaga catggccaac
     6361 aacatggggc tcaggtctgt cacttaactt agagtcttta catgaaatat cagaatggga
     6421 aagagaatgt tcctggtaaa actctgtttt cattaatttg ttatttttca aaaaccgagc
     6481 cacacctttg gaagtaatga tttggccatt ccagagattc tggggctaaa gtttgttttt
     6541 ccagggctgc tgatcctttt ccagatagct ccagagtatg atgaccccag ccccatccca
     6601 atctctagcg aaagggttat gcaaatctca gggtgttgcc tctaaagaat ctaaagaata
     6661 gacatgcttt gggcttactc agtgtaccag attctctagt gggccattct catttgatgt
     6721 tccctagaac ctccctgtct tccaactgca ggatgtgttt acttctcttc tgggcttccc
     6781 ctggtttctg tcctagttcc caccccaagt ttaagaagac ttagattcat ttattgctat
     6841 ctgaggccac tagtgagatt tcagatctct cttttgcaac attttaccca tttcccttcc
     6901 ccaaaccatg tgcttattag atggttccac tcaggaagca aggaagctaa aactcaagtt
     6961 aatggtctgt ggatcccacg aaagtgtgtg taaactactg actgtttctc cactagaggc
     7021 atttaattga gccaaagatg atggaagaag aaactctcaa agccatgtac tttgttaggt
     7081 gtctggattt ttggcctgtt tgcagcctct cctagccaca gacagacact gtctcctctc
     7141 agagaaaggc cagtgtttcg gtccaggcca ctggtgagtg tcggcaccct tgaggtgggc
     7201 atggagtgag cagaagaagc ctggagaaga gagaactatt taaaagtgtt gccagttatt
     7261 cagcttccag gcggctttgc acctccaaag gtgcaaaaag gaaaaagaaa gcagaaagga
     7321 ttcagcattt aagcagaaag aaaagggaaa ttctactcta ccagatgagc cactggttcc
     7381 actggagctt atcagtgaag taacactgca ggagccctgg tatccttccc tcagagatat
     7441 catcaggggc cctcaaaccc cattgtttat gccagcactg accgcacttt aagcacacac
     7501 ttaccaatca gaggtcgtgt aattggccta aatctaggcc cagttattac tacaaatctt
     7561 agtcaccata ctctctcgcc caggctggag tgcagtggtg tgatctcagc tcactgcaac
     7621 ctccacctcc caggctcaag ggatcctctc acctcagctc ccgagcagct aggactgcag
     7681 gtgtgcacca ccatgcccag ctaatttttg aatttttttg tagagatggg gttttgccat
     7741 gttgcccagg ctggtctgaa gctgctgggc tcaagcaatc cacctggctg ggcctcccaa
     7801 agcgatagga ttacaggcgt aagggcccac gcccagccta ccatactgat tttcaaggca
     7861 gggtctaacc cttcataaga tgtgagttct tccaacccaa taagcaggca gctttgagga
     7921 caaaaaggca acattaaaag ataagaccct agaagtcaga acatctgtat tccaaggcca
     7981 ccccttctcc ttttagtcat gtgactttgg gcaggtcacc ctccctgtct gtaaaatgag
     8041 gttggactgg ataatcttta tgtgcccttt ccttttggct taaaattctg tgatttattc
     8101 attcttagga agatcagcat atttattggg atttctgctt caaaaattct ttctaacctc
     8161 aaaaatccaa gtccagaatt ttttgttttc ttctggattt aaactgttta atatgagcac
     8221 atcaaggttg acttctcatc ctttttggga agtgaattta atattgaggg taattgaatg
     8281 atgttgcaat ggctttccct cctgctggct tggtaagaat attaaagcct gcagaggtgg
     8341 aaattggagc tcatggagaa atggatagaa agcaatttgt tgcaggcagc ctttggacaa
     8401 gttgttttaa taggaaatag acctgctgct tcataggttt cctcaaccac ctttcctcag
     8461 ctttcttaaa atgggatcta cattggctct tcacacccaa atagcagact aatcgttttt
     8521 ctgcttagca ccgtctggtt cattgtcttg aactctgcct tacagcagca agaaaatttt
     8581 cctcgacaag aacctcaatc tttagttcca ttgagctccc cctctggatt ttggacttac
     8641 cagaagtagg aggttctgat accattcaag atggtctttc cttcaaagca ggtctgaaga
     8701 ggagactacc aaagcagtgt ttacaaaccc agagtccaca caaccatatt gcatagaaca
     8761 gcacttggct ttcacaagcc tcctacagga cctggtgtaa ttggagtgaa agggcagaga
     8821 ccctggaagt ggaggtggct gtgtgctgcg atgggaagaa ggcagaaggc ccaggggctt
     8881 tggacataga gcagggtgga agctgcaagt actgggaagg aagagagttt cacagaaaca
     8941 aagctttgtc acacagaaat gagttctgtc tcactggtga cttcatccct caggctccag
     9001 ctgagcagag attttaatca gcttccttaa tgggtattga cactgctcag gaagcagtag
     9061 accctgtcag ggacagctat tgatcttttg tgttctgatt agattggaaa atagatcaac
     9121 ttcattgtag tccaggaact gttggtcaca gctactagga atgaggtgat ttctgagggc
     9181 tgagaaaaaa cacagaatct tggccagcag ccagcagctg catggtgaaa gatgcattca
     9241 cttctccttt gagagttggg gttgagggca aacatagaac ccaggtttgg cttacaaccc
     9301 agtgtcccgg aagccctcct tcgggagaac tgtaagtaag aggtgggtgt gtctaaagac
     9361 aataccatta atgaatgttc tggccttacc taaaaaggtt tagcaatttg gggataactc
     9421 ttggatctag cttatgtgcg ttcacatgca catttgctag cccagagctt ttaaaatgag
     9481 gtctggcata tacttgatta caaatgaaaa ctcagaaacc aattttattt attaaatcat
     9541 atcttttgtt tttccccctc ccttctaatc ccccaaagga cctatttgag ctgttcccca
     9601 attcatctgc ttattttgga ccatgaatct gccagagtga tattttctgt tatttctcct
     9661 ccaaattttt ccctgatgtt tccaataaag atttacttgg gtggcccctt aaggtgacat
     9721 caggatgctc ttatgtcctt ccagaataag catacacttc actcctctcc ctttcatctc
     9781 cctctgcatt cttaattcct tgcttttctc acttggagcc gagggtgctt tagagaggtg
     9841 gttttccatg aatcagccaa gattcctgta gaagttgggt ataccaacca ccaccaccac
     9901 cgccccctat tccagtttca aagctcctcg gctatgctaa tgtcccctca gagatgaggt
     9961 ttgactttta ggcccgtatg actcctccat agcctggcca aggagaccat gagtagccat
    10021 gtctggttta ctctttatcc tgagactgtt tatagcttaa aacagaagtg tgtcttccca
    10081 gcacaaacct aatcaatcag tgtatcagtg catctggtgg caacagctca gcccattcaa
    10141 agagcaagga ttcaggaaag gcacactgat ggtggggagc ctcttaagag cctctaatgt
    10201 tctcccaaaa ccagagttga gagtcggagt gccagtcgtc ggggcccact attcctgaat
    10261 aagggacatg caagggccag aagtagcttg actctcgcct aaatatctgt gcctttgcct
    10321 gtcctttctc ccactctact gaaacccgga acagattccc gcttgccttc tgatgaagag
    10381 aggttaggta aagagagttt ggaggaaaaa agacaccagg aggcaggctg cggggtagga
    10441 gagggttctg agaggaggca gcaatccaga atacctcctt ttctagccag catcccttga
    10501 acttttgaaa ggttgtgcct accactggct ggcacaccag ggcaatgatt tccctgcaga
    10561 aggaaggaaa gaatgtttca cccttgcatc cttcttggag aagctacagc ctgtgctcag
    10621 ttgagtggtt cacactcaga ctttggcttt atggttttcc ttcctccttg tctttgccct
    10681 gaccttgatc aacaggggtg aaaagaacca ccctgaggtt tccatgcctc tcccatttta
    10741 gtggtagcat tttgtgtctt tactccaccc ttcaccctag ttccaccaag gttcacacac
    10801 cagatgtaac tgtttttcag ctgagttgta tggattaact tcagtccact gtaaatacac
    10861 ctgggatggg gtggggttgg ggttgtttag ggagaagcag ccagacttgc tttgtgaact
    10921 gaatgtattt ttatgcaatt ttgagtggcc tttcaaccct aagatgaatg gttttgtttt
    10981 actggttgtt gttatatagt tttgagtatt ctgtgtttga aagtttggga ataataatat
    11041 tcacttctat atgatgcctg taaacacaac agtatttata aatgagtaaa taaaactgtg
    11101 ttttaacttt gtgactgtct ccacagttca gagcatggga tttctagaat ctcacatctg
    11161 gggcagcctc ttctgatttt acatcttatc attggtgggg gcctcgccca ccagcctcct
    11221 acaactcctg tccacataga ggccccttcc ccagggacag ccacagtgag tcatggccac
    11281 tgagaagggt ctctggtagt atcaaggaat ttcctaaatg gagtcaggcc aaggagaagc
    11341 ctagattggc gttcaaaaga accgtaagat agatagatag atagatagat agatagatag
    11401 atagatagat agatatcaag gttccaagct tcaagtaacc aagagtatat acggttattt
    11461 cttggaagac accaggttta agtgacaaag gtgttgaggc catccaggtg aacactagca
    11521 cactctagta catgggaaag ttgaggtcta gggaggtggt gatttccttg ggctcatgga
    11581 agtctcctgc catcaccctt agtcatctgc tatatccata agtcttcatg aggtcattgt
    11641 ttaattcagg agatccaaaa ctatcctgta gttccaaact acaaatgtag ttaacttggg
    11701 ggctattgaa ggttaaatct aaactcaaaa tgaaggagcc atgcccagag aagagctgtt
    11761 caggcccgct ggcagggttt gtacgtgggt ggccaggtct gaccccaggc agccaggatc
    11821 tgggtctggt ttctccatcc agcaagccct gcctctggct ttgctcagcc ctgtgtttcc
    11881 tgccaagcgg aaatccctgg tcactgactt ggggagaggt cagggaggga agattcttct
    11941 cccctgatgt cacccagctg tttgtaaccc aagcagcagc agaacaagca cacttgatgt
    12001 catggttgaa gaattcagtc ctgtcctggg gcacaaaaca gaggcaaaga agcatgaccg
    12061 atgcaaaggg ttcctagact gcaaatgtca tttctctttc tgacccccac tggttgtagt
    12121 aaggtctgca gatacagtat cacctagccg tcacgtggtc cagccctctt ggggggaaag
    12181 gagaaggcca caacctattg gcagagtaaa aagctcatat tcttagccag gagaccctca
    12241 ggtgataaat cccagtccgt ctgctgaaag gctcatgccc ctcaccctac ccagccttag
    12301 ggactgaagc agcaagacag gatagatgtt aaaggatctg ggctcatgct ccctcaggtg
    12361 ggcagctccc atccctgctt gcattcactg cccccatgcc tttggacctg atggacaagc
    12421 acacatctag ccaacggtgt tcctggagga caggtgctgg tgtaaactga caaaaatggt
    12481 agctgctggt ctatcctcaa ggagaggaga caggtggaag tgggaaccga agaaggagtt
    12541 ttactcttcc ctggtctttg ggggcaatgc tgtcccactc tccctttccc taaaaattct
    12601 gacgccctat ccctggtccc taacatcttt gcttcagaag ttaaagacat ttcaagttat
    12661 ttgcaaagag tcaactgagg actttgtaga aattgttact tttcaaattc ccacttccca
    12721 gggccagcct ccctctcgtc attgggctca gactgtggct gttccctaag cccagctcta
    12781 ctggaaggga attcaagaat atagtcccca ccaccacccc gccccgccca gcctcccaga
    12841 caatgttccc cggcaccacc acactcccca cagttccaca gcctggcatg tgttctcagg
    12901 agatgtgtgg gccccagcac ccaccagagt gccaatctgg tcttccccca accccaggag
    12961 agaggggacc tcagcccctt ttggtgcttc cctgaaggga gattctgggt tagaagccag
    13021 agcctcagga ccatctcacc accctcaccc accatactga ccctcacagg cagctcccac
    13081 tccctcaccc ccacctcagc gcagcaaaaa caaccagaga caccccccac ccccaggttt
    13141 ctttcagctc atttctttaa taaggagacg cagttcatta caaaataaca atttgacaag
    13201 agatcagaca agaacaagag tccacataag ggagatggag agcattgcca agcagaagtg
    13261 ggaatgagag ggccgggggc aagggctgta catgtgtcct tcctatggag acgaaggcag
    13321 ggctcctggt gggctgacct tggcgaagct aggctctgcc aggccctgat tttgaagttt
    13381 tcagccccag ggttttcaga aagcagcaaa tcaagtcctt agatgggcag gagtcagggc
    13441 agaagggtca ctatctttga agaggccccc ctaaagtcct gatcgctaag gcaggtggga
    13501 tggaggaact cctggtgccc catgcacact gctcccatca cctcaccaga cagatgctct
    13561 ctcaactctt gcctcaattt gtctctgctg tgggtgtgga gagtgggagg gccccagaga
    13621 gagctggagg aagcaagaca tgtcttcact ggtccaagca cctgattcca gcctgctctg
    13681 aaatggtccc ctccaccttc cccactcttt cagttcccag cacaaaggtc cctgggactt
    13741 ctgtgtgcaa cttctgttgt ggtttgtgca gggcaagcct gcctcgccac ctctggcctc
    13801 ctcgaatgcg ccctgcctga ttcccttccc gttctctctc cttcctcata cttgtcacag
    13861 ttgccaggcc tgaggcatcc tgggctcttc ccatgcagta ctgccctcag gtgcccatgg
    13921 ggccaggggc cgcaggcagg ggggctacct gtccctctca gttcaacagg atgacctggg
    13981 gccttggaag cccccaggcc cctctactca cctctgctac ttggtgcaac tggctggggc
    14041 ttctctgggg agcaggtgtg aaggtcaggg gacttagtcc acacctgctc tgcctgagcc
    14101 tctctcacag ctgaacctgc ccaccccact ctcaggggat cgctggtagc cctgaggttg
    14161 ccccgctatt gggctgcttt ctgtcagact gcaggaggat gcacgaggga agagcgtcag
    14221 cccctgtatt caccggcctc tccctgagtc cctcctccat caccacccac agaccctcac
    14281 actacagagg tgagtaagca gggatggttc tgtcatccct ggctgagctg ctgactggct
    14341 gctcctgaaa atcctggttg cctccacagg gaccccagga aaggtatcca gaaccatgga
    14401 gtcccactgc tgtctgctcc tcgcccagcg tagagatgga gctgaactgt gaacccaggc
    14461 cgagaaagcc agctcgggca gggccagcat gggcatctcc tgagacccca ccggaagccc
    14521 tggacaccgt ccattctcat gacacgggac tgagggagca ggagggagtg acaacaggtg
    14581 agcccttcag aagtggtctg tggggagcca gttggagaga catgggggct cctgggagga
    14641 gccaagggag gccaaatctc cagcaggagg ccccctttat ggcttttcag tgaattcaaa
    14701 cagtgggagg aaggggcaca ccacacacat acacagaaca tggaagctac tgggatgcca
    14761 gataccaggc tggacactgc accgggccct gaggtttggc tctgcaggca gatgtgcagc
    14821 aaacaggcct tcagggcgac agggtcctcc agcatctccc aaggcagggc tggactggag
    14881 caggcaccga ctcccgccca ctgatgactc ccatgggggg tttcccgatg ggaggaggca
    14941 ggggtggggc tggggaacaa agactttaca cgacataaaa tcaaaggagc agcctcaacc
    15001 agttctgtaa aatgggtttg aggttgggga tcaaggggac tctcagcatt gccatgcggc
    15061 tatttacaga aagttataga catgcatctt gattaaacaa gattctgttc atatgtttct
    15121 aggtaattat cttcttttat atataattaa atatacacgg atgggggcag atgaggctca
    15181 aatggcttca gaaggttctt attttgttgt tgcttaaaaa ataaagtaat tcatgtgtct
    15241 tcaa
//
