LOCUS       NM_016147               2487 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens protein phosphatase methylesterase 1 (PPME1),
            transcript variant 1, mRNA.
ACCESSION   NM_016147
VERSION     NM_016147.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ando,S., Tanaka,K., Matsumoto,M., Oyama,Y., Tomabechi,Y.,
            Yamagata,A., Shirouzu,M., Nakagawa,R., Okimoto,N., Taiji,M.,
            Sato,K. and Ohama,T.
  TITLE     The luciferase-based in vivo protein-protein interaction assay
            revealed that CHK1 promotes PP2A and PME-1 interaction
  JOURNAL   J Biol Chem 300 (5), 107277 (2024)
   PUBMED   38588804
  REMARK    GeneRIF: The luciferase-based in vivo protein-protein interaction
            assay revealed that CHK1 promotes PP2A and PME-1 interaction.
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Aakula,A., Isomursu,A., Rupp,C., Erickson,A., Gupta,N., Kauko,O.,
            Shah,P., Padzik,A., Pokharel,Y.R., Kaur,A., Li,S.P., Trotman,L.,
            Taimen,P., Rannikko,A., Lammerding,J., Paatero,I., Mirtti,T.,
            Ivaska,J. and Westermarck,J.
  TITLE     PP2A methylesterase PME-1 suppresses anoikis and is associated with
            therapy relapse of PTEN-deficient prostate cancers
  JOURNAL   Mol Oncol 17 (6), 1007-1023 (2023)
   PUBMED   36461911
  REMARK    GeneRIF: PP2A methylesterase PME-1 suppresses anoikis and is
            associated with therapy relapse of PTEN-deficient prostate cancers.
REFERENCE   3  (bases 1 to 2487)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2487)
  AUTHORS   Xu,T., Lei,T., Li,S.Q., Mai,E.H., Ding,F.H. and Niu,B.
  TITLE     DNAH17-AS1 promotes pancreatic carcinoma by increasing PPME1
            expression via inhibition of miR-432-5p
  JOURNAL   World J Gastroenterol 26 (15), 1745-1757 (2020)
   PUBMED   32351291
  REMARK    GeneRIF: DNAH17-AS1 promotes pancreatic carcinoma by increasing
            PPME1 expression via inhibition of miR-432-5p.
REFERENCE   5  (bases 1 to 2487)
  AUTHORS   Kauko,O., Imanishi,S.Y., Kulesskiy,E., Yetukuri,L., Laajala,T.D.,
            Sharma,M., Pavic,K., Aakula,A., Rupp,C., Jumppanen,M.,
            Haapaniemi,P., Ruan,L., Yadav,B., Suni,V., Varila,T.,
            Corthals,G.L., Reimand,J., Wennerberg,K., Aittokallio,T. and
            Westermarck,J.
  TITLE     Phosphoproteome and drug-response effects mediated by the three
            protein phosphatase 2A inhibitor proteins CIP2A, SET, and PME-1
  JOURNAL   J Biol Chem 295 (13), 4194-4211 (2020)
   PUBMED   32071079
  REMARK    GeneRIF: Phosphoproteome and drug-response effects mediated by the
            three protein phosphatase 2A inhibitor proteins CIP2A, SET, and
            PME-1.
REFERENCE   6  (bases 1 to 2487)
  AUTHORS   Migueleti,D.L., Smetana,J.H., Nunes,H.F., Kobarg,J. and
            Zanchin,N.I.
  TITLE     Identification and characterization of an alternatively spliced
            isoform of the human protein phosphatase 2Aalpha catalytic subunit
  JOURNAL   J Biol Chem 287 (7), 4853-4862 (2012)
   PUBMED   22167190
REFERENCE   7  (bases 1 to 2487)
  AUTHORS   Bachovchin,D.A., Mohr,J.T., Speers,A.E., Wang,C., Berlin,J.M.,
            Spicer,T.P., Fernandez-Vega,V., Chase,P., Hodder,P.S.,
            Schurer,S.C., Nomura,D.K., Rosen,H., Fu,G.C. and Cravatt,B.F.
  TITLE     Academic cross-fertilization by public screening yields a
            remarkable class of protein phosphatase methylesterase-1 inhibitors
  JOURNAL   Proc Natl Acad Sci U S A 108 (17), 6811-6816 (2011)
   PUBMED   21398589
  REMARK    GeneRIF: Academic cross-fertilization by public screening yields a
            remarkable class of protein phosphatase methylesterase-1
            inhibitors.
REFERENCE   8  (bases 1 to 2487)
  AUTHORS   Puustinen,P., Junttila,M.R., Vanhatupa,S., Sablina,A.A.,
            Hector,M.E., Teittinen,K., Raheem,O., Ketola,K., Lin,S., Kast,J.,
            Haapasalo,H., Hahn,W.C. and Westermarck,J.
  TITLE     PME-1 protects extracellular signal-regulated kinase pathway
            activity from protein phosphatase 2A-mediated inactivation in human
            malignant glioma
  JOURNAL   Cancer Res 69 (7), 2870-2877 (2009)
   PUBMED   19293187
  REMARK    GeneRIF: Observations identify PME-1 expression as one mechanism by
            which ERK pathway activity is maintained in cancer cells and
            suggest an important functional role for PME-1 in the disease
            progression of human astrocytic gliomas.
REFERENCE   9  (bases 1 to 2487)
  AUTHORS   Longin,S., Zwaenepoel,K., Martens,E., Louis,J.V., Rondelez,E.,
            Goris,J. and Janssens,V.
  TITLE     Spatial control of protein phosphatase 2A (de)methylation
  JOURNAL   Exp Cell Res 314 (1), 68-81 (2008)
   PUBMED   17803990
  REMARK    GeneRIF: We propose that stabilization of this inactive, nuclear
            PP2A pool is a major in vivo function of PME-1.
REFERENCE   10 (bases 1 to 2487)
  AUTHORS   Ogris,E., Du,X., Nelson,K.C., Mak,E.K., Yu,X.X., Lane,W.S. and
            Pallas,D.C.
  TITLE     A protein phosphatase methylesterase (PME-1) is one of several
            novel proteins stably associating with two inactive mutants of
            protein phosphatase 2A
  JOURNAL   J Biol Chem 274 (20), 14382-14391 (1999)
   PUBMED   10318862
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK022725.1 and AF157028.1.
            
            On Nov 23, 2018 this sequence version replaced NM_016147.2.
            
            Summary: This gene encodes a protein phosphatase methylesterase
            localized to the nucleus. The encoded protein acts on the protein
            phosphatase-2A catalytic subunit and supports the ERK pathway
            through dephosphorylation of regulatory proteins. It plays a role
            in malignant glioma progression. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Oct 2012].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the shorter isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.599365.1,
                                           SRR1803614.153902.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000328257.13/ ENSP00000329867.8
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                AK022725.1         37-70
            35-2487             AF157028.1         1-2453
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.4"
     gene            1..2487
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /note="protein phosphatase methylesterase 1"
                     /db_xref="GeneID:51400"
                     /db_xref="HGNC:HGNC:30178"
                     /db_xref="MIM:611117"
     exon            1..234
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             134..1294
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /EC_number="3.1.1.89"
                     /note="isoform a is encoded by transcript variant 1;
                     testicular secretory protein Li 39"
                     /codon_start=1
                     /product="protein phosphatase methylesterase 1 isoform a"
                     /protein_id="NP_057231.1"
                     /db_xref="CCDS:CCDS44678.1"
                     /db_xref="GeneID:51400"
                     /db_xref="HGNC:HGNC:30178"
                     /db_xref="MIM:611117"
                     /translation="MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPV
                     PWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISR
                     VQCRIVALDLRSHGETKVKNPEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGA
                     IAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKS
                     GQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESISKRKKEDD
                     METKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQ
                     MQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC"
     exon            235..328
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /inference="alignment:Splign:2.1.0"
     exon            329..421
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /inference="alignment:Splign:2.1.0"
     exon            422..479
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /inference="alignment:Splign:2.1.0"
     exon            480..531
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /inference="alignment:Splign:2.1.0"
     exon            532..686
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /inference="alignment:Splign:2.1.0"
     exon            687..777
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /inference="alignment:Splign:2.1.0"
     exon            778..843
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /inference="alignment:Splign:2.1.0"
     exon            844..967
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1097
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1142
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1207
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1275
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..2487
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2466..2471
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /note="hexamer: AATAAA"
     polyA_site      2487
                     /gene="PPME1"
                     /gene_synonym="ABDH19; PME-1"
                     /note="major polyA site"
ORIGIN      
        1 gctacgggta gctgggtgct gtccaaaggc gacagggcgt cgttagggga gcgagtcgtg
       61 accggttggg ccacactcaa cgtgggacga agcttcgcct actgtttgac tacgtgcgtg
      121 cagcctcccc tcgatgtcgg ccctcgaaaa gagcatgcac ctcggccgcc ttccctctcg
      181 cccacctcta cccggcagcg ggggcagtca gagcggagcc aagatgcgaa tgggccctgg
      241 aagaaagcgg gacttttccc ctgttccttg gagtcagtat tttgagtcca tggaagatgt
      301 agaagtagag aatgaaactg gcaaggatac ttttcgagtc tacaagagtg gttcagaggg
      361 tccagtcctg ctccttctgc atggaggagg tcattctgcc ctttcttggg ctgtgttcac
      421 ggcagcgatt attagtagag ttcagtgtag gattgtagct ttggatctgc gaagtcatgg
      481 tgaaacaaag gtcaagaatc ctgaagatct gtctgcagaa acaatggcaa aagacgttgg
      541 caatgtggtt gaagccatgt atggggacct tcctcctcca attatgctga ttggacatag
      601 catgggtggt gctattgcag tccacacagc atcatccaac ctggtaccaa gcctcttggg
      661 tctgtgcatg attgatgttg tagaaggtac agctatggat gcacttaata gcatgcagaa
      721 tttcttacgg ggtcgtccta aaaccttcaa gtctctggag aatgctattg aatggagtgt
      781 gaagagtggc cagattcgaa atctggagtc tgcccgtgtc tcaatggttg gccaagtcaa
      841 acagtgtgaa ggaattacaa gtccagaagg ctcaaaatct atagtggaag gaatcataga
      901 ggaagaagaa gaagatgagg aaggaagtga gtctataagc aagaggaaaa aggaagatga
      961 catggagacc aagaaagacc atccatacac ctggagaatt gaactggcaa aaacagaaaa
     1021 atactgggac ggctggttcc gaggcttatc caatctcttt cttagttgtc ccattcctaa
     1081 attgctgctc ttggctggtg ttgatagatt ggataaagat ctgaccattg gccagatgca
     1141 agggaagttc cagatgcagg tcctacccca gtgtggccat gcagtccatg aggatgcccc
     1201 tgacaaggta gctgaagctg ttgccacttt cctgatccgg cacaggtttg cagaacccat
     1261 cggtggattc cagtgtgtgt ttcctggctg ttagtgacct gctgtccacc cctcctcaac
     1321 atcgagctct gttgtaaata cgtcgcacca gaggccactg tgatgccact gtctcctctc
     1381 catcccgccc agccatgtga cactggctcc cggtagacgg gcaccccgag atgtaccaac
     1441 cttttcatgt attctgccaa aagcattgtt ttccagggcc cttgaccaac atcggcttcc
     1501 ccagtccagg gctcccctgc tcctttccct tccctgtact ggggtagctc ctgcctgctc
     1561 tccctgcgtt gcctagggta aagcctccag atttgccata ctgagcccct cttcctagca
     1621 tcaggcgata catctgagtt caaatgtctt cccaggctca gggacctcca ttccttgaga
     1681 ttgtcttggc atggcccagc cctgcctcat gggatggaca atgcatgggg tggtctttat
     1741 ttttcccttt caaataaaac actagtcagg taccgtttta tcccagtcgt actcttccag
     1801 gtttggaaga cccagagagg ccaagatccc atccttagcc atagcgagcg gtggtggtgg
     1861 atagcatcac aagaaacgag cctgaaaatc aggtccagcc ggtccaagca catggcctcc
     1921 catctgggag agcccactgt cccactccca catgtctggg cacctgccct gggctgaggc
     1981 caggctgctc caggggcctc ctgcgccctc acctgccaca gagcaaccca ggttaaatac
     2041 agcccatgca caaagccaca ggccaaagcc tatggaattg tttttaatca tcaaatttaa
     2101 ccattttcat aactggttcc tggaggtgtg cagtgccccc ttgcctcttc aaacctacag
     2161 cttctctttg ccatttgtgg atttcacatc actccacaca gaaacattac agcctggcat
     2221 ccccagtctt tgccttcttc cagctgcctc gacacagcac tgtggcctgt ccctattgcc
     2281 caggcacgcc atttccaagg gcaggaaggg gcagtgtcct gaagcccatc ttttctgtga
     2341 ctgtcttagg tgatgtgtag ccccctccac ctttccactc aacaacctcc cacccctgtc
     2401 ctgctgcatg gtccggagtc tgggacctac tttgtttttt gttatttatg accttgttta
     2461 aagaaaataa atatctccca accttta
//
