LOCUS       NM_139245              10906 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1L (PPM1L),
            transcript variant 1, mRNA.
ACCESSION   NM_139245
VERSION     NM_139245.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10906)
  AUTHORS   Merhi,Z., Polotsky,A.J., Bradford,A.P., Buyuk,E., Chosich,J.,
            Phang,T., Jindal,S. and Santoro,N.
  TITLE     Adiposity Alters Genes Important in Inflammation and Cell Cycle
            Division in Human Cumulus Granulosa Cell
  JOURNAL   Reprod Sci 22 (10), 1220-1228 (2015)
   PUBMED   25676576
REFERENCE   2  (bases 1 to 10906)
  AUTHORS   Lu,G., Ota,A., Ren,S., Franklin,S., Rau,C.D., Ping,P., Lane,T.F.,
            Zhou,Z.H., Reue,K., Lusis,A.J., Vondriska,T. and Wang,Y.
  TITLE     PPM1l encodes an inositol requiring-protein 1 (IRE1) specific
            phosphatase that regulates the functional outcome of the ER stress
            response
  JOURNAL   Mol Metab 2 (4), 405-416 (2013)
   PUBMED   24327956
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10906)
  AUTHORS   Tonevitsky,A.G., Maltseva,D.V., Abbasi,A., Samatov,T.R.,
            Sakharov,D.A., Shkurnikov,M.U., Lebedev,A.E., Galatenko,V.V.,
            Grigoriev,A.I. and Northoff,H.
  TITLE     Dynamically regulated miRNA-mRNA networks revealed by exercise
  JOURNAL   BMC Physiol 13, 9 (2013)
   PUBMED   24219008
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10906)
  AUTHORS   Shinoda,Y., Fujita,K., Saito,S., Matsui,H., Kanto,Y., Nagaura,Y.,
            Fukunaga,K., Tamura,S. and Kobayashi,T.
  TITLE     Acyl-CoA binding domain containing 3 (ACBD3) recruits the protein
            phosphatase PPM1L to ER-Golgi membrane contact sites
  JOURNAL   FEBS Lett 586 (19), 3024-3029 (2012)
   PUBMED   22796112
  REMARK    GeneRIF: identified ACBD3 as an interacting partner of PPM1L, and
            showed that this association, which recruits PPM1L to ER-Golgi
            membrane contact sites, is mediated by a GOLD (Golgi dynamics)
            domain in ACBD3
REFERENCE   5  (bases 1 to 10906)
  AUTHORS   Thean,L.F., Loi,C., Ho,K.S., Koh,P.K., Eu,K.W. and Cheah,P.Y.
  TITLE     Genome-wide scan identifies a copy number variable region at 3q26
            that regulates PPM1L in APC mutation-negative familial colorectal
            cancer patients
  JOURNAL   Genes Chromosomes Cancer 49 (2), 99-106 (2010)
   PUBMED   19847890
  REMARK    GeneRIF: Genetic variation at chromosome 3 harbors an element that
            regulates expression of an upstream candidate tumor suppressor,
            PPM1L, thus providing a novel mechanism for colorectal
            tumorigenesis.
REFERENCE   6  (bases 1 to 10906)
  AUTHORS   Saito,S., Matsui,H., Kawano,M., Kumagai,K., Tomishige,N.,
            Hanada,K., Echigo,S., Tamura,S. and Kobayashi,T.
  TITLE     Protein phosphatase 2Cepsilon is an endoplasmic reticulum integral
            membrane protein that dephosphorylates the ceramide transport
            protein CERT to enhance its association with organelle membranes
  JOURNAL   J Biol Chem 283 (10), 6584-6593 (2008)
   PUBMED   18165232
  REMARK    GeneRIF: CERT is a physiological substrate of PP2Cepsilon and that
            dephosphorylation of CERT by PP2Cepsilon may play an important role
            in the regulation of ceramide trafficking from the ER to the Golgi
            apparatus.
REFERENCE   7  (bases 1 to 10906)
  AUTHORS   Saito,J., Toriumi,S., Awano,K., Ichijo,H., Sasaki,K., Kobayashi,T.
            and Tamura,S.
  TITLE     Regulation of apoptosis signal-regulating kinase 1 by protein
            phosphatase 2Cepsilon
  JOURNAL   Biochem J 405 (3), 591-596 (2007)
   PUBMED   17456047
  REMARK    GeneRIF: These results suggest that PP2Cepsilon maintains ASK1 in
            an inactive state by dephosphorylation in quiescent cells,
            supporting the possibility that PP2Cepsilon and PP5 play different
            roles in H2O2-induced regulation of ASK1 activity.
REFERENCE   8  (bases 1 to 10906)
  AUTHORS   Jin,F., Ji,C., Liu,L., Dai,J., Gu,S., Sun,X., Xie,Y. and Mao,Y.
  TITLE     Molecular cloning and characterization of a novel human protein
            phosphatase 2C cDNA (PP2C epsilon*)
  JOURNAL   Mol Biol Rep 31 (3), 197-202 (2004)
   PUBMED   15560375
  REMARK    GeneRIF: cloning and characterization of a novel human protein
            phosphatase 2C cDNA (PP2C epsilon*)
REFERENCE   9  (bases 1 to 10906)
  AUTHORS   Li,M.G., Katsura,K., Nomiyama,H., Komaki,K., Ninomiya-Tsuji,J.,
            Matsumoto,K., Kobayashi,T. and Tamura,S.
  TITLE     Regulation of the interleukin-1-induced signaling pathways by a
            novel member of the protein phosphatase 2C family (PP2Cepsilon)
  JOURNAL   J Biol Chem 278 (14), 12013-12021 (2003)
   PUBMED   12556533
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY337264.1, AC021649.18 and
            AW274846.1.
            
            On Nov 22, 2018 this sequence version replaced NM_139245.3.
            
            Summary: The protein encoded by this gene is a magnesium or
            manganese-requiring phosphatase that is involved in several
            signaling pathways. The encoded protein downregulates apoptosis
            signal-regulating kinase 1, a protein that initiates a signaling
            cascade that leads to apoptosis when cells are subjected to
            cytotoxic stresses. This protein also is an endoplasmic reticulum
            transmembrane protein that helps regulate ceramide transport from
            the endoplasmic reticulum to the Golgi apparatus. Finally, this
            gene may be involved in adiposity since it is upregulated in
            adipose tissues. Several transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Dec
            2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.33845.1,
                                           SRR1803612.170966.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000498165.6/ ENSP00000417659.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1717              AY337264.1         24-1740
            1718-10721          AC021649.18        115092-124095       c
            10722-10906         AW274846.1         13-197              c
FEATURES             Location/Qualifiers
     source          1..10906
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q25.33-q26.1"
     gene            1..10906
                     /gene="PPM1L"
                     /gene_synonym="PP2C-epsilon; PP2CE; PPM1-LIKE"
                     /note="protein phosphatase, Mg2+/Mn2+ dependent 1L"
                     /db_xref="GeneID:151742"
                     /db_xref="HGNC:HGNC:16381"
                     /db_xref="MIM:611931"
     exon            1..477
                     /gene="PPM1L"
                     /gene_synonym="PP2C-epsilon; PP2CE; PPM1-LIKE"
                     /inference="alignment:Splign:2.1.0"
     CDS             79..1161
                     /gene="PPM1L"
                     /gene_synonym="PP2C-epsilon; PP2CE; PPM1-LIKE"
                     /EC_number="3.1.3.16"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein phosphatase 1L; PP2C epsilon"
                     /codon_start=1
                     /product="protein phosphatase 1L isoform 1"
                     /protein_id="NP_640338.2"
                     /db_xref="CCDS:CCDS33886.1"
                     /db_xref="GeneID:151742"
                     /db_xref="HGNC:HGNC:16381"
                     /db_xref="MIM:611931"
                     /translation="MIEDTMTLLSLLGRIMRYFLLRPETLFLLCISLALWSYFFHTDE
                     VKTIVKSSRDAVKMVKGKVAEIMQNDRLGGLDVLEAEFSKTWEFKNHNVAVYSIQGRR
                     DHMEDRFEVLTDLANKTHPSIFGIFDGHGGETAAEYVKSRLPEALKQHLQDYEKDKEN
                     SVLSYQTILEQQILSIDREMLEKLTVSYDEAGTTCLIALLSDKDLTVANVGDSRGVLC
                     DKDGNAIPLSHDHKPYQLKERKRIKRAGGFISFNGSWRVQGILAMSRSLGDYPLKNLN
                     VVIPDPDILTFDLDKLQPEFMILASDGLWDAFSNEEAVRFIKERLDEPHFGAKSIVLQ
                     SFYRGCPDNITVMVVKFRNSSKTEEQ"
     exon            478..652
                     /gene="PPM1L"
                     /gene_synonym="PP2C-epsilon; PP2CE; PPM1-LIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            653..814
                     /gene="PPM1L"
                     /gene_synonym="PP2C-epsilon; PP2CE; PPM1-LIKE"
                     /inference="alignment:Splign:2.1.0"
     exon            815..10906
                     /gene="PPM1L"
                     /gene_synonym="PP2C-epsilon; PP2CE; PPM1-LIKE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8457..8462
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPM1L"
                     /gene_synonym="PP2C-epsilon; PP2CE; PPM1-LIKE"
                     /note="hexamer: AATAAA"
     polyA_site      8484
                     /gene="PPM1L"
                     /gene_synonym="PP2C-epsilon; PP2CE; PPM1-LIKE"
                     /note="major polyA site"
     regulatory      10837..10842
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPM1L"
                     /gene_synonym="PP2C-epsilon; PP2CE; PPM1-LIKE"
                     /note="hexamer: AATGAA"
     regulatory      10879..10884
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPM1L"
                     /gene_synonym="PP2C-epsilon; PP2CE; PPM1-LIKE"
                     /note="hexamer: AATAAA"
     polyA_site      10880
                     /gene="PPM1L"
                     /gene_synonym="PP2C-epsilon; PP2CE; PPM1-LIKE"
     polyA_site      10906
                     /gene="PPM1L"
                     /gene_synonym="PP2C-epsilon; PP2CE; PPM1-LIKE"
                     /note="major polyA site"
ORIGIN      
        1 gtccccgcag tgctcccgga cccggcgagc cttcggggcg cgcgtcgctg gtggtggttg
       61 aggctctagc gataataaat gatagaggat acaatgactt tgctgtctct gctgggtcgc
      121 atcatgcgct acttcttgct gagacccgag acgcttttcc tgctgtgcat cagcttggct
      181 ctatggagtt acttcttcca caccgacgag gtgaagacca tcgtgaagtc cagccgggac
      241 gccgtgaaga tggtgaaggg caaggtagcc gagatcatgc agaacgatcg actcgggggg
      301 cttgatgtgc tcgaggccga gttttccaag acctgggagt tcaagaacca caacgtggcg
      361 gtgtactcca tccagggccg gagagaccac atggaggacc gcttcgaagt tctcacggat
      421 ctggccaaca agacgcaccc gtccatcttc gggatcttcg acgggcacgg gggagagact
      481 gcagctgaat atgtaaaatc tcgactccca gaggccctta aacagcatct tcaggactac
      541 gagaaagaca aagaaaatag tgtattatct taccagacca tccttgaaca gcagattttg
      601 tcaattgacc gagaaatgct agaaaaattg actgtatcct atgatgaagc aggcacaacg
      661 tgtttgattg ctctgctatc agataaagac ctcactgtgg ccaacgtggg tgactcgcgc
      721 ggggtcctgt gtgacaaaga tgggaacgct attcctttgt ctcatgatca caagccttac
      781 cagttgaagg aaagaaagag gataaagaga gcaggtggtt tcatcagttt caatggctcc
      841 tggagggtcc agggaatcct ggccatgtct cggtccctgg gggattatcc gctgaaaaat
      901 ctcaacgtgg tcatcccaga cccagacatc ctgacctttg acctggacaa gcttcagcct
      961 gagttcatga tcttggcatc agatggtctc tgggatgctt tcagcaatga agaagcagtt
     1021 cgattcatca aggagcgctt ggatgaacct cactttgggg ccaagagcat agttttacag
     1081 tcattttaca gaggctgccc tgacaatata acagtcatgg tggtgaagtt cagaaatagc
     1141 agcaaaacag aagagcagtg aacccttcag gggtctcagc tgccttagac taaaggactt
     1201 tcaacacact ggtctctttt aatttagtga aaagtgtggg agttgtaatt aggatcatcc
     1261 accccagaca tggaatcccc cctccctggt ggtcttaggt ctataatcag tgacgaacag
     1321 agggtgccct tggccaatgt agttaagaaa ctggaaaatg gtttcttcat gttttcccaa
     1381 ctctttcatc cagtgtccaa aatatataag taaatagctg tagagtcaca tatatgaagt
     1441 gaatagcata tgtgtcattt agtctccctg aagattcttt tcaagatcct gttcagggtc
     1501 ctccaggcat cagctgttgt gtcctctctt tgtaacagtg gacaggacag accacccagt
     1561 gctgcaggag acaggccact gcgtcacctg tgagtggtca ggggctgatg tggcaacacc
     1621 ctctgccaag agacagagct gtcctgagaa tgctttgtcc ttctgagccc atgttttctg
     1681 ctcagtagca gcttggaagc agatttggaa tggtttacta ttttggctgc tcttggggac
     1741 tgcgagaagc agagagaatg agagaccagt ggcaactgcc tgcacagcag agataaccct
     1801 cttcccttgc ttcctttaat agttaaatag actttgtata ccacctgacc agcctttgtg
     1861 catttatcct aatcatgcat gaccgttaac cttttgctta gtccttacca tatgtaatag
     1921 gcagctgtta aattcaccaa cagataccct gatttttcat cttacgtgac caagaaacca
     1981 cgttagggga aatgaaaaaa gcaagccaca ataccatgat tccttccatt ttcaacagta
     2041 gatgaaggaa atgatactga atgagtcaca gtgttccctg gcaagtaagc tgtttgcatt
     2101 gagaaaggag tgagctggtg aggttaccac cctgaattga gctccagctg ccagtttttg
     2161 tgtttttcct tgcccctttc caagtggttt tcaagtgtca ggcagtgttc tgagaagcag
     2221 cagcctataa ctgtatgtgt gttccttgaa gccaggtgca gagttcccag ctactgcagc
     2281 ttgggatttg gtgggaaact actgggataa gcttctcctt gacaatggaa aggcagcagt
     2341 cttcaacatt tggttgcaaa tctccatcca catcagggag ctttccccag gcaaatacaa
     2401 accgccccgt ggcctgcagg cctgcagggg aggcagcaaa gggacctggc agttgcaaca
     2461 cagtaagtca ggaattcaag cgtgaaccca tattgataag tgggccagaa ttatttaggg
     2521 gcctggcttg ctgctctcca tttcctgtat cgctaattta tcatttttcc aaccttggta
     2581 ttttatatta tttagagaaa atgctgggtc actctctttg cctaaggtga ctcaaacagc
     2641 caaacgaaac ttccgttgcc tcccagccct ctgtatccac ctggttttgt tctttccctg
     2701 tgagcatttg tggttatagc ttcccatatg ccacattcac cggccacctc ctcgccgtgg
     2761 aaccctgccc tctcccctcc tgctgctgta gagttttccc aaagcagtgt ccaggagaga
     2821 atttgagaga gtgaagaaat tgccttgtag atgtggaggg ctgcaatctg tcttgatttc
     2881 tccaagcact taactttctt tgactgcact gagaattgct aatgatttcc catgagattt
     2941 gcttactttt gtatactgta ttttccagca ttacagaacc ttggttattg ttttttagcc
     3001 atagaatctt ctagtaaaaa atatctgcca ccattttaga tttaagcatt tgcctatggg
     3061 gagacactga atatgtggat gtgtgtatta atatttgggg tggggacagg gaagggaatg
     3121 tggaaaacaa atgctggctg tgagcagtgc tgagatggcc aggccaggcg gctgagtttg
     3181 cttggaaatt caggacattc tgactcctaa gagttgcccc cacccaccat caaactgaaa
     3241 tcagcaccaa tggtgtcagc actttacagc ccatagccaa ctttctttat ttttaacgta
     3301 gcacaaaaat gtataatagc aaggaaaaga catttttaaa ttccggttat ttttattgtc
     3361 taaaatgaaa gcaacagtgt tttgataaag atgaaaaaga aaagctacta aattagtaaa
     3421 tcagtggtta cgtgccctgc agaatttctt aacagatggt gctgagtgca cgagttacat
     3481 aactttctct ctaattgagg ttcacaaggc gtcttctaaa ttttgctttg tacaattaat
     3541 tcatttctga tgttaaccaa atagagtgta tatatcctac tcccattact gcctctttcc
     3601 cccctactat ggcttgtaga ttttcaaaag atagaagttc taggcaaaac tgtagcagtt
     3661 tcattaatag ttgttaggat agttatatct aaaatcagag tattttgttc ccttccttct
     3721 ggagctctct ctccctagcc cttttccagc tgttggggat gtgcagacat cactcctggg
     3781 ggtgccctat ggtacactgg ctgctcccca gggacctggg aatgctgaat ctagccatta
     3841 gggaaattga tttgagatgt ttgcatgtga agcttcccta agcagcactg ccaaagcttt
     3901 ggaattgtca cccaaggctt cctagactcc ctagctagct cgagttcgaa ttgccaatag
     3961 tccccatggt gccaaatttt gggatggttt tacagtcttt tagaaatgaa gacaggaatg
     4021 gccatgtgct tctgagaccc cagcacgatg ccaggcccag gtctccatta atgcagtgct
     4081 ctgctgctgc tccactggca atttgtactc ttaacatagg ttgggggagg gacagctagg
     4141 gaaatttttt ttaattaatt gtatctaaaa ttctttcatc ataggaataa acaacacata
     4201 tagaaagctc caaagctgtt cccaagacct gtattattta ttttatatga ccaacttgcc
     4261 ccaaggcaat taactctgaa ttgctgttct aatgtgacat atctgtgtac atatgtacat
     4321 agtactggta accagtggct gcattttatt ccttgctgtg gactgaggta ttggcttctt
     4381 gaatcttgta tatgtcataa gaatattata cagagagtgt tatagtgaag gatgtaagct
     4441 ggataattta aaacagacct tatttcatac aagtgtaatt attacatcat tttgggtaaa
     4501 tggcaaacag gattgcatga gagtgatggc atcatcattt caattgaatc tgaagtgatt
     4561 ctaggaaatt gattccactg ttggattcta ggaaattgag tccactgttt tcctttgcgt
     4621 ttaatttcct ttgtgtttaa tttgaccaca gaaacttttt tgagtagcac ctagtaacat
     4681 tgcaaggaac ttgtgtatca aggtgtatct tgattatcct gatttttttt gttctctaag
     4741 ttgttgccag atctctcttg caactgtgtc aatgaaaggt ctgtgtttag aaaaggcagc
     4801 atatcattgt atatttgaaa ctataggaat atatactttg tataaaactt ttccaatgat
     4861 ttcagaaatt cttttttcct ccttttgacc cacaactaga ctgttcatac cctaatagtt
     4921 ccccaaaatt gccttagcat gtcacaccag catttgtcat ccagctctgg agaaatgaca
     4981 tgactgttag atactatgcc tgtttaattg cctctggatt aagtcattga tagctagact
     5041 tttgagctag ttagctgtag aaataataga atccagtgtt tcccaaagtg tgttccaaag
     5101 aacacaagtt ccaaagatgc tcttctaaat ataggattct aggatgaaat aagtttggaa
     5161 aacacatacc atcttagaaa gttagtgaac atgagcacat caaaggctct aatttaaatg
     5221 tcctgtgagg agagaaaaac acaacaaata tctactgtca gttagcccag tgttttccag
     5281 attaatttga ccacagaaac ttttttgagt ggcacctagt aacactgcaa ggaacttgtg
     5341 ttctaaagaa caccctttgg gaaattctgg tatattgaaa tattgctgtg ttttcattca
     5401 cgatgactct tagtagcagt acaatttgca acttagaagc atgagccttt catatatgaa
     5461 gctgacttgt taataaaagc agtgttaaga agtagtatga cttaatatga ccaacagcag
     5521 cctcatattg atagcagaac aatcctactt aaagctctaa acatcatccc cccctttttt
     5581 tttttaacgg aatctcgctc tgtcacccag gctggaatgc cgtggcgcaa tgtcaggtca
     5641 ctgcaagctc cgcctcccag gttcacacca ttctcctgcc tcagcctcct gagcagctgg
     5701 gactacaggc ggcctccacc acacctggct aattttttgt atttttagta gagacggggt
     5761 ttcaccatgt tagccaggat ggtcttgata tcctgacctt gtaatccacc agccttggcc
     5821 tcccaaagtg ctgggattac aggtgtgagc cactgcaccc ggccatccct ttcttttata
     5881 tgaataatga gcaagagccc tgccatagct aaatatgtct caaattcatt ccaagatctt
     5941 tccattgctt ttttgcagtt acctttgctt tttgggttga atcaaaatga atatttttat
     6001 atttcattac tatatgatat ttaaaaatat acagaaaatc acagaaaagg aaaaacagaa
     6061 atttgatttt aattcacttt tgaaatttta acgattttta aaactaatga tctgttatat
     6121 aataactgaa atgtaaacta ttaacagtta tttactttct ttcctttatt gtcctctctg
     6181 gattcagtga aataatttga aatcaattag agctcatacc ttctaaaact cctgtcccat
     6241 atcatcccac ctttcatgtt ttatgtacat agttaagttt ggcatgttat ctcttgccta
     6301 aaatgtgagg ccttcctgta gttccacagt agttactctt ggctgcagaa ataggtttgg
     6361 gaagctatga gagattaccc caaagtcatg gatgaaacaa atctagtcga aaacatggta
     6421 cagagtgaat taaggcaaaa gtcattaact taaaattaca tccaatattt aggaatagct
     6481 catcctctgc acattatcca aaatatttta aaaaactaaa agtaagatct cttaggctag
     6541 gtggcggatt gcaaagggca gggggcagtt gataccatga agcttccaat agatgcattt
     6601 ctaaacattt tttctaatat gtaaatttgc tgatccttag taatgttaat gctttgtcta
     6661 tttgttattt ttaccactgt tgaacagcaa actgttgagg ctagggttga ttattgataa
     6721 tttttatgtg tttagtaata accacagcat catacgtaca actgctttaa taaatccaag
     6781 ttaaggactt tgaggagagc atgtagcaag tattcatttt aacaaatcat cacagacagt
     6841 ttccctgttc aaagctccac caaaaagcaa gcatgcagca aaggcataga ctgtatttag
     6901 agatgtggtt tcatttattc ttgaagtcct ataaccttgc attatttaaa cacaaaatcc
     6961 cacctaacat ttgccaaatc caagttaatc tcaaaaacct ccgaggacat tgagcacaag
     7021 cagattactg taagggcact ggtggcagat tgtggtggag agagggctga gtgacccaga
     7081 gcaaagcctg cccgggttac tcgctgccac ttcaggagag ggattgtatc agctctcatt
     7141 accttttgtc atgaggcagt tcataaatta aatatgatat catgcattat ctcaaaatat
     7201 ttctatatta attagataat tcacgatgta aaacgtgttc atgcagaagc aaaggcggat
     7261 gtaatagtca agtatcaggg aaaaacccct ttaggatacc cagacattaa aaatgtaaaa
     7321 agtagcttat aagaaatgtg gattatcaga tttttactat atataggttg tgtttaatag
     7381 taatttctga cacctgcaca tagatgaaga aaaggcagtt gttaccactt ttcaccattc
     7441 aaaaaatggg cttgtctctt agagtattgg ttaatgcttt gcttatttgt taatgaagac
     7501 agcattttaa ttttaaagct tccttttgta ggactgatgg cacgggcaga atcacattta
     7561 agaaaaatgg tttggaacac tttttaataa aaaattgtaa aatcagtttc cagtgtggtc
     7621 aaaattagaa tgtaaaggaa agcattttta agaaaataca gaagcccaaa tcaagggaga
     7681 gtaatataaa aggaaatatt ttaaattaag aaaaggaggt gttatagttt attacatcat
     7741 gaattatgct ttctgtactt acgctcttag tgaaggagat aacattgaag tgagaaaatg
     7801 aacattctca gttatttgcg aagttagtaa aattgcagtc cctattccat ttttttctga
     7861 tgaaaatgga tacatgagat ctcatttatt gctctcctca acacctttac tgtcccagat
     7921 tccattcacg ttccaaatcg tggacagtaa aactgaagtc agataaacac agtcacaggt
     7981 acaactggga gcgagtttta acatagtggt aattttgcaa atgccaacat gaatactagt
     8041 gagtactcaa tttacgacag acaatttcac aaaaaccaac cacagagtga tttgatattc
     8101 ttgcttctta gagagttttc taatccatat aatgcaatgg gtcaggttgc tcatatgatt
     8161 aaaaaaatta atcctgtaga aatgatgtat tagacatctc taacccaaga aaaagatgtc
     8221 ttttgtataa cttttaaatt cccatttgtc tatcttagga caaatgatga cttcttcgtg
     8281 tagtaatgaa aacgtctaaa tcctgctctg tggagaaaag ctagaaccat gggacattaa
     8341 gcaagaatac tcctctgatt taggctccca gactacaact aacagtcttt atgtatttat
     8401 catcttggtt attgttttat ctcaagcata tacagtgtgc agaaagtaga ataatgaata
     8461 aaatatgacc atggagaaaa cccataactt ttgcttctat tatagaaagc agtgtttgtt
     8521 ctttttaatt tattacatgt tcacattgaa acattaacat ctacaatatt gtctttcagt
     8581 ttactcttca attactaaag caaatgattt tgatttatag cacttacaca actgcagttc
     8641 aggtcacaga attgaaatct atttgataat gtctcagcat tctcatgggc agccaggaaa
     8701 aaaaaaaaga gagagaaaga caaatagaaa ttttagtggg ttcagataaa tgattattct
     8761 gacaaacctg gaaaatagta attaaacagt tcattccaaa agaccccaat gtaaactctg
     8821 ctcacataca taatttgtat aaggaaaatg tatgagtttt tttcttaaaa ataaatagaa
     8881 gcatcattgc atttataaca gattgatcaa tcttatgaac agattacatt gggaaagtca
     8941 ctattattgc tgtgtgtttg tatattttat tctttccttt tttgtgtgag catctactat
     9001 gtgccaggca gtgactgtgt ctgagtgaat gtatccatgg tgagcactca ggcgccatca
     9061 ctgccttctt ggggcttaca ccctattggg gtgtcagaga tattataaca ctcaatattg
     9121 acccaatggg atgaaaattg ttatcttcat gatttcttcc aatgggctta gatttgttgg
     9181 gacagggcag aattagaata attatttctc acatataaga gtaagtgata tgagattggg
     9241 aatttcttgc caacactcaa gcaaactgca gaagataatt actccatttt actagctcac
     9301 ctagtttctg aaggatgggg tggggagtga gacatggagc ctctggggac aaggataggg
     9361 gggtgggact gtcccagtgg agggtgccac tggaagcctc cacccaagtg ggccagcagc
     9421 tgtgcaaggg tgcagcagta ggctggtgaa agccaggttg tccagatctt tctaggggaa
     9481 gagaaggatt atagaaaagg agaaaaggga ggagcaagaa catctctcca tggcctattt
     9541 ttatttttgt tcccaagaga ctagtgtaat aaccttatgc ccccaaagac agcccagaac
     9601 tgagtatctt cagtgttcct gagtgtaaat cagtgaatct gttatataga aagttaacat
     9661 ttcaggagca caattatttc ctgatttcaa atatgaacag aagactaaat gtcatttttt
     9721 taaatcataa atttggtatc catagttact ttacacattc cagagtctaa gcatggaatt
     9781 taagttttgt attttgaaca aaaatagcaa catctgtttt ctgtatggca aagaaaaact
     9841 aaatcccaga cttttatttt taaccacaga aaccatcact tctttctaaa ttatttgctt
     9901 gcctgctcat ttcccctaaa catctctgtt tatgccaatg gctttttaaa tccaaataat
     9961 actagttatt gccaaattgc attagaatcc tattgaatta gaataattca tatacttcat
    10021 atactaggca gtaaatggtt agagacggta aagatctgat tgtgatcaaa agtagggttt
    10081 ttgttttgtt tttaatcatg agcacatata aaaggaaatt caaatgaaac caagccaatt
    10141 tctgatttag taagattaaa accatagatg gagctcagtt attaaggatg gaaaggagga
    10201 aagcacagag tgaggagaga aaaaggggaa ggaaaaaaag tagtgttttt ttggggaaca
    10261 gtataatgtc tcagaagttc ttctctctta tcctgctgat gttgagacag tggctttgta
    10321 taagctttta tttataccta tttcaacatc atgaaaattt ttccatcagc attttcttgc
    10381 aactttaaaa atcacaaggg attatttgtt tataaagaga tacaggatct ataatttatt
    10441 ggcaatactt tattttagga atcttcaaat tgtacttaat aaataaatca caggtcaaaa
    10501 tgactgtaaa atcaatcagg ctttctcgta agttatttcc tgaaaaccat ccatgataaa
    10561 tacaacaagg cttggcgaaa cctcattact cttggaagtc ttcagaggtt attataccta
    10621 ctgatctgta tttgtctcta agagacattt gaaacacagt tacaatgtca atttggtgta
    10681 acattacctg tagtagagct cagagtatat ggcagtgcac ggtcactcaa gatgcatggc
    10741 gtgtcatttg ggtcgctttg atgggtgctc gggtagaatt agccttgtag agactaatgt
    10801 gttcatgcta tctctgatcc tgtggtgttg tataaaaatg aacagctaaa aatttaccca
    10861 tgacaagatt aaagcaaaaa taaacacaaa gttgttagac tttaaa
//
