LOCUS       NM_152542               6309 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1K (PPM1K),
            mRNA.
ACCESSION   NM_152542
VERSION     NM_152542.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Mu,L., Ye,Z., Hu,J., Zhang,Y., Chen,K., Sun,H., Li,R., Mao,W.,
            Long,X., Zhang,C., Lai,Y., Liu,J., Zhao,Y. and Qiao,J.
  TITLE     PPM1K-regulated impaired catabolism of branched-chain amino acids
            orchestrates polycystic ovary syndrome
  JOURNAL   EBioMedicine 89, 104492 (2023)
   PUBMED   36863088
  REMARK    GeneRIF: PPM1K-regulated impaired catabolism of branched-chain
            amino acids orchestrates polycystic ovary syndrome.
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 6309)
  AUTHORS   Hu,W., Liu,Z., Yu,W., Wen,S., Wang,X., Qi,X., Hao,H., Lu,Y., Li,J.,
            Li,S. and Zhou,H.
  TITLE     Effects of PPM1K rs1440581 and rs7678928 on serum branched-chain
            amino acid levels and risk of cardiovascular disease
  JOURNAL   Ann Med 53 (1), 1316-1326 (2021)
   PUBMED   34382495
  REMARK    GeneRIF: Effects of PPM1K rs1440581 and rs7678928 on serum
            branched-chain amino acid levels and risk of cardiovascular
            disease.
REFERENCE   4  (bases 1 to 6309)
  AUTHORS   Neumann,J., Boknik,P., Kirchhefer,U. and Gergs,U.
  TITLE     The role of PP5 and PP2C in cardiac health and disease
  JOURNAL   Cell Signal 85, 110035 (2021)
   PUBMED   33964402
  REMARK    GeneRIF: The role of PP5 and PP2C in cardiac health and disease.
            Review article
REFERENCE   5  (bases 1 to 6309)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 6309)
  AUTHORS   Cronin,S., Tomik,B., Bradley,D.G., Slowik,A. and Hardiman,O.
  TITLE     Screening for replication of genome-wide SNP associations in
            sporadic ALS
  JOURNAL   Eur J Hum Genet 17 (2), 213-218 (2009)
   PUBMED   18987618
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
REFERENCE   7  (bases 1 to 6309)
  AUTHORS   Sonuga-Barke,E.J., Lasky-Su,J., Neale,B.M., Oades,R., Chen,W.,
            Franke,B., Buitelaar,J., Banaschewski,T., Ebstein,R., Gill,M.,
            Anney,R., Miranda,A., Mulas,F., Roeyers,H., Rothenberger,A.,
            Sergeant,J., Steinhausen,H.C., Thompson,M., Asherson,P. and
            Faraone,S.V.
  TITLE     Does parental expressed emotion moderate genetic effects in ADHD?
            An exploration using a genome wide association scan
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 147B (8), 1359-1368 (2008)
   PUBMED   18846501
REFERENCE   8  (bases 1 to 6309)
  AUTHORS   Joshi,M., Jeoung,N.H., Popov,K.M. and Harris,R.A.
  TITLE     Identification of a novel PP2C-type mitochondrial phosphatase
  JOURNAL   Biochem Biophys Res Commun 356 (1), 38-44 (2007)
   PUBMED   17336929
  REMARK    GeneRIF: The cloned phosphatase has a mitochondrial leader sequence
            and its amino acid sequence places it in the PP2C family like two
            known mitochondrial phosphatases.
            Erratum:[Biochem Biophys Res Commun. 2007 Oct 5;361(4):1061]
REFERENCE   9  (bases 1 to 6309)
  AUTHORS   Lu,G., Ren,S., Korge,P., Choi,J., Dong,Y., Weiss,J., Koehler,C.,
            Chen,J.N. and Wang,Y.
  TITLE     A novel mitochondrial matrix serine/threonine protein phosphatase
            regulates the mitochondria permeability transition pore and is
            essential for cellular survival and development
  JOURNAL   Genes Dev 21 (7), 784-796 (2007)
   PUBMED   17374715
REFERENCE   10 (bases 1 to 6309)
  AUTHORS   Dai,J., Zhang,J., Sun,Y., Wu,Q., Sun,L., Ji,C., Gu,S., Feng,C.,
            Xie,Y. and Mao,Y.
  TITLE     Characterization of a novel human protein phosphatase 2C family
            member, PP2Ckappa
  JOURNAL   Int J Mol Med 17 (6), 1117-1123 (2006)
   PUBMED   16685424
  REMARK    GeneRIF: A novel member of human protein phosphatase 2C gene family
            named PP2Ckappa was isolated from a human fetal brain cDNA library.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY435431.1, AC108213.6 and
            AC097484.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_152542.4.
            
            Summary: This gene encodes a member of the PPM family of
            Mn2+/Mg2+-dependent protein phosphatases. The encoded protein,
            essential for cell survival and development, is targeted to the
            mitochondria where it plays a key role in regulation of the
            mitochondrial permeability transition pore. [provided by RefSeq,
            Sep 2012].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY994097.1, AL834271.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000608933.6/
                                                  ENSP00000477341.1
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1702              AY435431.1         176-1877
            1703-2812           AC108213.6         1-1110              c
            2813-6309           AC097484.3         164723-168219       c
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q22.1"
     gene            1..6309
                     /gene="PPM1K"
                     /gene_synonym="BCKDH; BDP; MSUDMV; PP2Ckappa; PP2Cm; PTMP;
                     UG0882E07"
                     /note="protein phosphatase, Mg2+/Mn2+ dependent 1K"
                     /db_xref="GeneID:152926"
                     /db_xref="HGNC:HGNC:25415"
                     /db_xref="MIM:611065"
     exon            1..156
                     /gene="PPM1K"
                     /gene_synonym="BCKDH; BDP; MSUDMV; PP2Ckappa; PP2Cm; PTMP;
                     UG0882E07"
                     /inference="alignment:Splign:2.1.0"
     exon            157..655
                     /gene="PPM1K"
                     /gene_synonym="BCKDH; BDP; MSUDMV; PP2Ckappa; PP2Cm; PTMP;
                     UG0882E07"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..1334
                     /gene="PPM1K"
                     /gene_synonym="BCKDH; BDP; MSUDMV; PP2Ckappa; PP2Cm; PTMP;
                     UG0882E07"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.52"
                     /note="protein phosphatase 2C kappa; PP2C-type
                     mitochondrial phosphoprotein phosphatase; protein
                     phosphatase 1K, mitochondrial; PP2C-kappa; PP2C
                     domain-containing protein phosphatase 1K; branched-chain
                     alpha-ketoacid dehydrogenase phosphatase; branched-chain
                     I+/--ketoacid dehydrogenase phosphatase; protein
                     phosphatase 2C family member; [3-methyl-2-oxobutanoate
                     dehydrogenase
                     (2-methylpropanoyl-transferring)]-phosphatase,
                     mitochondrial"
                     /codon_start=1
                     /product="protein phosphatase Mn(2+)-dependent 1K"
                     /protein_id="NP_689755.3"
                     /db_xref="CCDS:CCDS3629.1"
                     /db_xref="GeneID:152926"
                     /db_xref="HGNC:HGNC:25415"
                     /db_xref="MIM:611065"
                     /translation="MSTAALITLVRSGGNQVRRRVLLSSRLLQDDRRVTPTCHSSTSE
                     PRCSRFDPDGSGSPATWDNFGIWDNRIDEPILLPPSIKYGKPIPKISLENVGCASQIG
                     KRKENEDRFDFAQLTDEVLYFAVYDGHGGPAAADFCHTHMEKCIMDLLPKEKNLETLL
                     TLAFLEIDKAFSSHARLSADATLLTSGTTATVALLRDGIELVVASVGDSRAILCRKGK
                     PMKLTIDHTPERKDEKERIKKCGGFVAWNSLGQPHVNGRLAMTRSIGDLDLKTSGVIA
                     EPETKRIKLHHADDSFLVLTTDGINFMVNSQEICDFVNQCHDPNEAAHAVTEQAIQYG
                     TEDNSTAVVVPFGAWGKYKNSEINFSFSRSFASSGRWA"
     exon            656..756
                     /gene="PPM1K"
                     /gene_synonym="BCKDH; BDP; MSUDMV; PP2Ckappa; PP2Cm; PTMP;
                     UG0882E07"
                     /inference="alignment:Splign:2.1.0"
     exon            757..922
                     /gene="PPM1K"
                     /gene_synonym="BCKDH; BDP; MSUDMV; PP2Ckappa; PP2Cm; PTMP;
                     UG0882E07"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1067
                     /gene="PPM1K"
                     /gene_synonym="BCKDH; BDP; MSUDMV; PP2Ckappa; PP2Cm; PTMP;
                     UG0882E07"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1202
                     /gene="PPM1K"
                     /gene_synonym="BCKDH; BDP; MSUDMV; PP2Ckappa; PP2Cm; PTMP;
                     UG0882E07"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..6309
                     /gene="PPM1K"
                     /gene_synonym="BCKDH; BDP; MSUDMV; PP2Ckappa; PP2Cm; PTMP;
                     UG0882E07"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6287..6292
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPM1K"
                     /gene_synonym="BCKDH; BDP; MSUDMV; PP2Ckappa; PP2Cm; PTMP;
                     UG0882E07"
                     /note="hexamer: AATAAA"
     polyA_site      6309
                     /gene="PPM1K"
                     /gene_synonym="BCKDH; BDP; MSUDMV; PP2Ckappa; PP2Cm; PTMP;
                     UG0882E07"
                     /note="major polyA site"
ORIGIN      
        1 gagaatttcc agcaggcaag gcagtggccg ctttgactgc ttgcttcgga gatccgagac
       61 gacggagaag gcactcttat ttaccgacca agaaagctcc tcccccgtcc tccgttagct
      121 aattaaaaca tttttcaggg acgtagccat ccagagacat tccattattg ttccattgac
      181 ctttccctca tcactgagtc ctttggagct gagttatgtc aacagctgcc ttaattactt
      241 tggtcagaag tggtgggaac caggtgagaa ggagagtgct gctaagctcc cgcctgctgc
      301 aggacgacag gcgggtgaca cccacgtgcc acagctccac ttcagagcct aggtgttctc
      361 ggtttgaccc agatggtagt gggagtccag ctacctggga caattttggg atctgggata
      421 accgcattga tgagccaatt ctgctgccac ccagcattaa gtatggcaag ccaattccca
      481 aaatcagctt ggaaaatgtg gggtgcgcct cacagattgg caaacggaaa gagaatgaag
      541 atcggtttga cttcgctcag ctgacagatg aggtcctgta ctttgcagtg tatgatggac
      601 acggtggacc tgcagcagct gatttctgtc atacccacat ggagaaatgt attatggatt
      661 tgcttcctaa ggagaagaac ttggaaactc tgttgacctt ggcttttcta gaaatagata
      721 aagccttttc gagtcatgcc cgcctgtctg ctgatgcaac tcttctgacc tctgggacta
      781 ctgcaacagt agccctattg cgagatggta ttgaactggt tgtagccagt gttggggaca
      841 gccgggctat tttgtgtaga aaaggaaaac ccatgaagct gaccattgac catactccag
      901 aaagaaaaga tgaaaaagaa aggatcaaga aatgtggtgg ttttgtagct tggaatagtt
      961 tggggcagcc tcacgtaaat ggcaggcttg caatgacaag aagtattgga gatttggacc
     1021 ttaagaccag tggtgtcata gcagaacctg aaactaagag gattaagtta catcatgctg
     1081 atgacagctt cctggtcctc accacagatg gaattaactt catggtgaat agtcaagaga
     1141 tttgtgactt tgtcaatcag tgccatgatc ccaacgaagc agcccatgcg gtgactgaac
     1201 aggcaataca gtacggtact gaggataaca gtactgcagt agtagtgcct tttggtgcct
     1261 ggggaaaata taagaactct gaaatcaact tctcattcag cagaagcttt gcctccagtg
     1321 gacgatgggc ctgattacca gctgggactt agagtttctg tgcaacagtt tttcactgag
     1381 catgtcaaga aactgataag atcaaaaagg tctcctaact cactagatca gcgcacaagt
     1441 cagtgtaaac cacttagata gtagtttttt cataaatgct catcatattt atgttccgct
     1501 gtacatgttc agtataaata tatgtgtagt gaagctactg tgagtcttta aatggaaaga
     1561 gcaaatgaga agtggtttgg atacacttga tgagagatga gagtgtcaca ttaataattt
     1621 ttaagactct taggcagcta tgggtttctt ttgatcattt ttgttcttta ttcatttgaa
     1681 cacgtttttg aagttcttca aaactagtca gtttgaattt tgacagctat tcaatatgtg
     1741 atctccaagt ttaaaaaaat ttttttccag acttccctaa tcctaaaatg cgagttttta
     1801 tttttaataa ctgtaccaag gaataagtat gaaaacagtt ctctgttacc atattttgta
     1861 ttctggacca cttactggtg aaagcaacca tgcaaaagaa attaatttgg ccaggcacag
     1921 tggctcatgc ctgtaatccc agcactttga gaggccaagg tgggtagatc atctgaggtc
     1981 aggaattcaa gaccagcctg gccaacatgg tgaaaccctg tctctagtaa aaatccaaaa
     2041 aaaaaaaaaa aaaaaaaaaa aaaattggct gggcgtggtg gcagacacct gtaatcccag
     2101 ctactcggga ggctgaggaa agaggaatca cttgaaccca ggagatgggg gttgcagtga
     2161 gctgagatca tgccattgca ctccagcctg ggcagcaaga gcgaaaaact ccatctcaaa
     2221 aaaaaaaaaa aaaagaaaga aaagaaatta attcagatga tgtgacatta ctaaattgta
     2281 tacatattta taaatgtgta tttcagctgt ctcatcagcc ctcccctcca tttattccta
     2341 tttttctgta gttaagaata ctgtaaaaat gtgactattc cttaatatca gaaaagaatg
     2401 cacaggctga gtcttccggt caaaagttaa atactgatgg aatcaagtat tttgtatgaa
     2461 gttctcattt gttatctcta acgctatttt gtgttttgca tatagtatta tagtatgtgt
     2521 atatcacttt ttgtatagag taagcaattg aataatttgt taataaaaat aataccacat
     2581 tgactgatac tgctatagac atagcttgag ttttatgtct cctttttgct cattttctaa
     2641 gacttaggag aaagaataaa tctaaaatga ccattaaaac attctccatt tcagcttgct
     2701 gtgtaactta ggaagtataa aactatactt ctctttatct gcatgtaagt ttgctgttaa
     2761 aatgtgaatt tctaaatgtg tatttggaat tatttccgta gtttatttct gcaaactatg
     2821 taaatttgtt atatgtgtga gtatgtgtac atatgtatat tttagagtaa tataaaatta
     2881 aagggaacaa tcttgcatag ctctttcacc agttttaaat tattgatacg ttatttttaa
     2941 ggactcttga caaactaggg gcaattttct tatagtggag acccagtgat tatcagcaga
     3001 tgaggaaata ggttaaaaat tgctatatgg caatttgtat ataaagtaat aggatgtgag
     3061 aaaaatactg aatcttaaga atgacatgga attctgtggc agaaacaaaa gaaaaagttg
     3121 atgtagtata taccttcaac tgtgttttga gttgactttt ttttttttct ttaagctttg
     3181 ggtaaaaatg tatgaggagg gagagggccc aatataaata tttacatctt cttacctttt
     3241 tggaaatgga agaagataaa tcttgagttt ttctgcctat attaatcagg aattgccctt
     3301 tgaaaaaagt gctaaataaa tattttgatt ttttttttca agggaagtta ggtgaaagaa
     3361 ggaaaaacat cacaggaaag actgttaaca ttctgtttgt tgtctgagag gtgaggaacc
     3421 aaagggaggc aactaaagcg ggaaaccatc ttgctttttc taatcagttc ttggaacaga
     3481 aatgtggaag ctacctttag gaacatggag aatttccaaa ccaacaggca aaggaaaact
     3541 aacgcacaaa aatgacattc tgaagatgca ggtttcagcc aggcgcggtg gctcaagcct
     3601 gtaatcctag cactttggga ggccaaggca ggtggatcac ctgaggtcaa gagttagaga
     3661 ccagcctggc caacatggtg aaacctcatc ttgaccaaaa aatgcaaaaa ttagccaggc
     3721 gtggtggtgg gtgcctgtaa tcccagctac tggggaggct gaggaaggag aattgcttga
     3781 acctgggagg cggaggttgc agtgagtcga gatcgcgcca ttgcactcca gcctggacag
     3841 cagagcaaaa actccatctc agataaataa ataaacaaat atgcaggttt catttttgtt
     3901 ttgaatgctt tacattacta ttcctatctt ttactttaaa aactaagatg aggagtgatg
     3961 ttgtcaagtt caaaggcatg agggtgattg atggattgag gtagatagcc aagtttgtct
     4021 gtttttgttt tttatagtta cagagtctca ctttgttgtc caggctgaag tgcagtggtg
     4081 caatcatagc tcactgagat tgccaggttc gacaaagagg aatttatagg atggggatat
     4141 agggtagact tgactctgct ttatccggga aagcttttaa aactctgagc cagttaactt
     4201 tgagtaagca taaaacatac tgtattggtg tttgtatttt tcatgccaca atattaaaat
     4261 ggaattttaa atgtagatta ttataatcta taaaagataa gtatgcatgt attaggatac
     4321 tggaaaatat gcaaatcata gtaaaaaaaa agggactgct ctagtttttc agttataact
     4381 gaatttgcca tgtgggtata ggcatagtgt aaattacatt aatgtagtaa aacatcaatt
     4441 gtggttcggt ttgtctttca tttatgtgta gtatagaaat cacctttcta atatgtgttg
     4501 ccaaactatt tgccaccatc tatttggtga aatattcatt gtcattgtga ttttccacaa
     4561 gtataagttc ttaagtactt tatagattca gaagtaaatg ctgtcctgtt ctccatcagc
     4621 tgttcgtttg ttacagattt tgtatttctt tttttttttt tttgagatgg agtctcactc
     4681 tgtcacacag gctggagtac agtggtgcgg tctcggctcc ctgcaacctc tgcctcccgg
     4741 gttcaaacga ttcttctgcc tcagcctcct gagtagctgg gactacaggc gcgtgccacc
     4801 acccctggct aatttttgta tttttttttc tttttttttt ttgagaagga gtctctctct
     4861 gtcacccagg ctggagtgca gtggcacgat ctcggctcac tgcaagctgc gcctcctggg
     4921 ttcacgccat tctcctgcct cagcctcccg agtagctgga attacaggcg tccgccaccg
     4981 tgcccggcta atttttttgt atttttagta gagatggggt tttgccatat tggccaggct
     5041 ggtctcgaac tcctgacctc aagtgatcca cccacctcgg cctcccaaag tgctgggatt
     5101 acaggcatga gccaccgcac ctggccagat ctttgtatgt cttaagtgtt tcaaagttat
     5161 aagcattttt ctggggggat gtccattttg gagggatcca ttttgatcct ttgtactcta
     5221 taatgtgaac tttcccctgt tccaacactt aaaagagaat tattagcaca taatctaaaa
     5281 gatggaattt tttttttttc ttgagacaga gtctcgctct gtcgccaggc tggagtgcag
     5341 tggcgcgatc ttggctcact gcaacctctg cctcctgggt ttaagcgatt ctcctgcctc
     5401 agcctctgga gtagctggga ctactcgtgc atgccaccac gcccggctaa tttttgtatt
     5461 tttagtagag acagggtttc accatattgg ccaggatggt ctcgatctct tgacctcgtg
     5521 atccacctgc ctcggcctcc caaattgctg ggattacagc actgtgccct cctaggaaat
     5581 tattttttaa gtgaaatttt atttttattt tttttaggat tttggtagag aatgagtagg
     5641 cctactcatc aatatcaaac aggacattta gtttctttcc ttagaacaga cataaattta
     5701 atttcatggt aatatgataa taagaaaatg cttctatttt tctttagcac ctccatggtt
     5761 ctcatatacc catgtctgta aaaagtgaca tgagaatttt gttgggttac attttattgt
     5821 atttattaga ttcgcttata tagatgactt aggcagaaat aaagtcatgt ctttagaagg
     5881 tgaacaagcc aacttgtgat ggcctgcctt ttgcttttgg cagttgggat gagaacaatt
     5941 gactctccca ttggttgtta gatagttgaa atggtgcgtt ggtggtcata cttagtgttc
     6001 taggctgtga aatcatggag ttcttccact tccaagaatg actcatttgc tgttggattc
     6061 tagtacagaa tttagcagcc tgatgtgtcc ccaaactgat ttaatttcta ctgaagtgcc
     6121 cttgtgtaca tttgttttgt aatttaccaa agtactacct gagtgtataa tgactcctgc
     6181 agtgagttaa tgtaattgct gctttgacca ttgttttaaa tctgtgtact agagtaactg
     6241 tgagcagaat gaaatcacat tatctcagtg ttcaaaatat cattctaata aagtacatgc
     6301 attaaacaa
//
