LOCUS       NM_014906               6560 bp    mRNA    linear   PRI 07-JUL-2024
DEFINITION  Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E),
            transcript variant 1, mRNA.
ACCESSION   NM_014906
VERSION     NM_014906.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Gheibi,S., Cataldo,L.R., Hamilton,A., Huang,M., Kalamajski,S.,
            Fex,M. and Mulder,H.
  TITLE     Reduced Expression Level of Protein Phosphatase PPM1E Serves to
            Maintain Insulin Secretion in Type 2 Diabetes
  JOURNAL   Diabetes 72 (4), 455-466 (2023)
   PUBMED   36662636
  REMARK    GeneRIF: Reduced Expression Level of Protein Phosphatase PPM1E
            Serves to Maintain Insulin Secretion in Type 2 Diabetes.
REFERENCE   2  (bases 1 to 6560)
  AUTHORS   Chen,M.B., Liu,Y.Y., Cheng,L.B., Lu,J.W., Zeng,P. and Lu,P.H.
  TITLE     AMPKalpha phosphatase Ppm1E upregulation in human gastric cancer is
            required for cell proliferation
  JOURNAL   Oncotarget 8 (19), 31288-31296 (2017)
   PUBMED   28423719
  REMARK    GeneRIF: Ppm1E upregulation in human gastric cancer is important
            for cell proliferation, possible via regulating AMPK-mTOR
            signaling.
REFERENCE   3  (bases 1 to 6560)
  AUTHORS   Li,Z.W., Zhu,Y.R., Zhou,X.Z., Zhuo,B.B. and Wang,X.D.
  TITLE     microRNA-135b expression silences Ppm1e to provoke AMPK activation
            and inhibit osteoblastoma cell proliferation
  JOURNAL   Oncotarget 8 (16), 26424-26433 (2017)
   PUBMED   28460435
  REMARK    GeneRIF: our results suggest that miR-135b-induced Ppm1e silence
            induces AMPK activation to inhibit osteoblastoma cell
            proliferation.
REFERENCE   4  (bases 1 to 6560)
  AUTHORS   Li,P., Fan,J.B., Gao,Y., Zhang,M., Zhang,L., Yang,N. and Zhao,X.
  TITLE     miR-135b-5p inhibits LPS-induced TNFalpha production via silencing
            AMPK phosphatase Ppm1e
  JOURNAL   Oncotarget 7 (47), 77978-77986 (2016)
   PUBMED   27793001
  REMARK    GeneRIF: miR-135b-5p expression downregulates Ppm1e to activate
            AMPK signaling, which inhibits LPS-induced TNFalpha production via
            suppressing ROS production and NFkappaB activation.
REFERENCE   5  (bases 1 to 6560)
  AUTHORS   Fan,J.B., Ruan,J.W., Liu,W., Zhu,L.Q., Zhu,X.H., Yi,H., Cui,S.Y.,
            Zhao,J.N. and Cui,Z.M.
  TITLE     miR-135b expression downregulates Ppm1e to activate AMPK signaling
            and protect osteoblastic cells from dexamethasone
  JOURNAL   Oncotarget 7 (43), 70613-70622 (2016)
   PUBMED   27661114
  REMARK    GeneRIF: Data show that miR-135b selectively targets the AMPK
            phosphatase Ppm1e.
REFERENCE   6  (bases 1 to 6560)
  AUTHORS   Sueyoshi,N., Takao,T., Nimura,T., Sugiyama,Y., Numano,T.,
            Shigeri,Y., Taniguchi,T., Kameshita,I. and Ishida,A.
  TITLE     Inhibitors of the Ca(2+)/calmodulin-dependent protein kinase
            phosphatase family (CaMKP and CaMKP-N)
  JOURNAL   Biochem Biophys Res Commun 363 (3), 715-721 (2007)
   PUBMED   17897624
REFERENCE   7  (bases 1 to 6560)
  AUTHORS   Ishida,A., Tada,Y., Nimura,T., Sueyoshi,N., Katoh,T., Takeuchi,M.,
            Fujisawa,H., Taniguchi,T. and Kameshita,I.
  TITLE     Identification of major Ca(2+)/calmodulin-dependent protein kinase
            phosphatase-binding proteins in brain: biochemical analysis of the
            interaction
  JOURNAL   Arch Biochem Biophys 435 (1), 134-146 (2005)
   PUBMED   15680915
REFERENCE   8  (bases 1 to 6560)
  AUTHORS   Takeuchi,M., Taniguchi,T. and Fujisawa,H.
  TITLE     Identification and characterization of nuclear localization signals
            of CaMKP-N
  JOURNAL   J Biochem 136 (2), 183-188 (2004)
   PUBMED   15496589
REFERENCE   9  (bases 1 to 6560)
  AUTHORS   Koh,C.G., Tan,E.J., Manser,E. and Lim,L.
  TITLE     The p21-activated kinase PAK is negatively regulated by POPX1 and
            POPX2, a pair of serine/threonine phosphatases of the PP2C family
  JOURNAL   Curr Biol 12 (4), 317-321 (2002)
   PUBMED   11864573
  REMARK    GeneRIF: The p21-activated kinase PAK is negatively regulated by
            POPX1 and POPX2, a pair of serine/threonine phosphatases of the
            PP2C family. (POPX1; POPX2)
REFERENCE   10 (bases 1 to 6560)
  AUTHORS   Takeuchi,M., Ishida,A., Kameshita,I., Kitani,T., Okuno,S. and
            Fujisawa,H.
  TITLE     Identification and characterization of CaMKP-N, nuclear
            calmodulin-dependent protein kinase phosphatase
  JOURNAL   J Biochem 130 (6), 833-840 (2001)
   PUBMED   11726284
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC025521.21, AK289966.1 and
            AC100832.2.
            
            On Nov 22, 2018 this sequence version replaced NM_014906.4.
            
            Summary: This gene encodes a member of the PPM family of
            serine/threonine-protein phosphatases. The encoded protein is
            localized to the nucleus and dephosphorylates and inactivates
            multiple substrates including serine/threonine-protein kinase PAK
            1, 5'-AMP-activated protein kinase (AMPK) and the multifunctional
            calcium/calmodulin-dependent protein kinases. Alternatively spliced
            transcript variants have been observed for this gene. [provided by
            RefSeq, May 2012].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and is protein-coding.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.165465.1, BC151228.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2145245
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000308249.4/ ENSP00000312411.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                AC025521.21        80887-80901         c
            16-275              AK289966.1         1-260
            276-1851            AK289966.1         267-1842
            1852-6560           AC100832.2         20031-24739         c
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q22"
     gene            1..6560
                     /gene="PPM1E"
                     /gene_synonym="caMKN; CaMKP-N; CAMKPN; POPX1; PP2CH"
                     /note="protein phosphatase, Mg2+/Mn2+ dependent 1E"
                     /db_xref="GeneID:22843"
                     /db_xref="HGNC:HGNC:19322"
                     /db_xref="MIM:619308"
     exon            1..608
                     /gene="PPM1E"
                     /gene_synonym="caMKN; CaMKP-N; CAMKPN; POPX1; PP2CH"
                     /inference="alignment:Splign:2.1.0"
     CDS             145..2412
                     /gene="PPM1E"
                     /gene_synonym="caMKN; CaMKP-N; CAMKPN; POPX1; PP2CH"
                     /EC_number="3.1.3.16"
                     /note="partner of PIX 1; nuclear calmodulin-dependent
                     protein kinase phosphatase; protein phosphatase 1E (PP2C
                     domain containing); caMKP-nucleus; partner of PIXA;
                     partner of PIX-alpha; ca(2+)/calmodulin-dependent protein
                     kinase phosphatase N"
                     /codon_start=1
                     /product="protein phosphatase 1E"
                     /protein_id="NP_055721.3"
                     /db_xref="CCDS:CCDS11613.1"
                     /db_xref="GeneID:22843"
                     /db_xref="HGNC:HGNC:19322"
                     /db_xref="MIM:619308"
                     /translation="MAGCIPEEKTYRRFLELFLGEFRGPCGGGEPEPEPEPEPEPEPE
                     SEPEPEPELVEAEAAEASVEEPGEEAATVAATEEGDQEQDPEPEEEAAVEGEEEEEGA
                     ATAAAAPGHSAVPPPPPQLPPLPPLPRPLSERITREEVEGESLDLCLQQLYKYNCPSF
                     LAAALARATSDEVLQSDLSAHYIPKETDGTEGTVEIETVKLARSVFSKLHEICCSWVK
                     DFPLRRRPQLYYETSIHAIKNMRRKMEDKHVCIPDFNMLFNLEDQEEQAYFAVFDGHG
                     GVDAAIYASIHLHVNLVRQEMFPHDPAEALCRAFRVTDERFVQKAARESLRCGTTGVV
                     TFIRGNMLHVAWVGDSQVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWR
                     VNGSLSVSRAIGDAEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVV
                     SDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRDMNKAVNVSEESDWTENSF
                     QGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFTDRTSLSPGSQINVLED
                     PGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKANLINELMMEKKSVQSSLPEWSGAG
                     EFPTAFNLGSTGEQIYRMQSLSPVCSGLENEQFKSPGNRVSRLSHLRHHYSKKWHRFR
                     FNPKFYSFLSAQEPSHKIGTSLSSLTGSGKRNRIRSSLPWRQNSWKGYSENMRKLRKT
                     HDIPCPDLPWSYKIE"
     exon            609..727
                     /gene="PPM1E"
                     /gene_synonym="caMKN; CaMKP-N; CAMKPN; POPX1; PP2CH"
                     /inference="alignment:Splign:2.1.0"
     exon            728..927
                     /gene="PPM1E"
                     /gene_synonym="caMKN; CaMKP-N; CAMKPN; POPX1; PP2CH"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1116
                     /gene="PPM1E"
                     /gene_synonym="caMKN; CaMKP-N; CAMKPN; POPX1; PP2CH"
                     /inference="alignment:Splign:2.1.0"
     exon            1117..1260
                     /gene="PPM1E"
                     /gene_synonym="caMKN; CaMKP-N; CAMKPN; POPX1; PP2CH"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1354
                     /gene="PPM1E"
                     /gene_synonym="caMKN; CaMKP-N; CAMKPN; POPX1; PP2CH"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..6560
                     /gene="PPM1E"
                     /gene_synonym="caMKN; CaMKP-N; CAMKPN; POPX1; PP2CH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6535..6540
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPM1E"
                     /gene_synonym="caMKN; CaMKP-N; CAMKPN; POPX1; PP2CH"
                     /note="hexamer: AATAAA"
     polyA_site      6560
                     /gene="PPM1E"
                     /gene_synonym="caMKN; CaMKP-N; CAMKPN; POPX1; PP2CH"
                     /note="major polyA site"
ORIGIN      
        1 ctctccaggc aacctagtgc tgatcgctcg tgccggtgcg gccgttaacc gcccttgccg
       61 gagccctagg ctcaaaagca gccccttacc cttcctgggc ttcccccaac ccctttcccg
      121 gtctgccctg gggcatgagc agcgatggcc ggctgcatcc ctgaggagaa aacttaccgg
      181 cgcttcctgg agctattcct gggcgagttt cgcggaccgt gcggcggcgg cgagccggag
      241 ccggaacccg aacccgaacc cgaacccgaa cccgagtccg agcccgagcc cgaacctgaa
      301 ctggtagaag ctgaggcggc cgaggcttcg gtagaggaac ccggggagga ggcggccacg
      361 gtagccgcga cggaggaggg ggaccaggag caagacccgg agcccgagga ggaggcggcg
      421 gttgagggtg aggaggagga ggagggcgcg gcgacggcgg cggcagcccc ggggcactcg
      481 gccgtgccgc cgccgccgcc ccagctgccg cctttgcccc cgctcccgcg accgctgtca
      541 gagcgcatca cccgcgagga ggtggagggc gaaagcctgg acctgtgcct gcagcagctc
      601 tacaaatata attgcccttc ctttttggct gctgctttag ccagagccac atcagatgaa
      661 gtccttcaga gtgatctttc tgcacattat atcccaaagg aaacggatgg cacagaaggg
      721 actgtggaga ttgagacagt gaaattggcc cgttctgtct tcagcaaact acacgagatt
      781 tgctgcagct gggtgaaaga cttccccctc cgcaggagac cccagcttta ttatgagaca
      841 tcaatccatg ccatcaaaaa catgcgcagg aaaatggagg acaaacatgt ctgcattcct
      901 gactttaata tgctcttcaa cctagaggac caggaagaac aagcttactt tgcagtgttt
      961 gatggccatg ggggagtaga tgctgctatt tatgcctcca ttcacctcca cgttaactta
     1021 gtccgccagg agatgttccc ccatgatcct gctgaggccc tgtgcagggc cttccgggtc
     1081 actgatgagc ggtttgtgca gaaagcagcc agggagagct taagatgtgg gaccacagga
     1141 gtggtgactt tcatcagagg caacatgcta catgtggcct gggtgggtga ttcccaggtt
     1201 atgcttgtga gaaagggcca agctgttgaa ctaatgaagc cacacaaacc agacagagag
     1261 gatgaaaagc agagaattga ggcccttgga ggttgcgtag tctggtttgg tgcctggagg
     1321 gtgaatggaa gtctgtcggt ttccagagct attggagatg ctgaacataa gccatatatc
     1381 tgtggggatg cagattctgc ctccactgtt ctggatggga ccgaagacta cctcattctg
     1441 gcctgtgatg gcttctatga caccgtgaac cctgatgagg cagtgaaagt tgtgtccgac
     1501 cacctgaaag agaataatgg agacagcagc atggttgccc acaaattagt ggcatcagct
     1561 cgtgatgctg ggtcaagtga taacatcacg gttattgtgg tattcctgag ggacatgaac
     1621 aaagctgtaa atgttagtga ggaatcagat tggacagaga actcttttca aggagggcaa
     1681 gaagatggtg gggatgataa ggagaatcat ggagagtgca aacgcccttg gcctcagcac
     1741 cagtgctcag caccagccga cctaggctat gatgggcgtg tggattcatt cactgataga
     1801 actagcctga gcccagggtc ccaaatcaac gtgctggaag acccaggcta cctagatctc
     1861 acacaaatag aagcaagcaa acctcacagt gcccagtttt tgctaccagt tgagatgttt
     1921 ggtcctggtg caccaaagaa agcaaatctt attaatgagt taatgatgga gaaaaaatca
     1981 gttcagtcat cattgcctga atggagtggt gctggagagt ttcccactgc tttcaatttg
     2041 ggttcaacag gggagcagat atacagaatg cagagcttgt ctcctgtctg ttcagggttg
     2101 gaaaatgaac agttcaaatc cccgggaaac agagtttcta gattgtctca tttacgccac
     2161 cactactcaa agaagtggca cagattcagg tttaatccaa agttttattc atttctctct
     2221 gctcaagagc cttcccacaa aataggcact agcctgtcct cacttactgg aagtgggaag
     2281 agaaatagga taagaagttc tctgccatgg aggcaaaata gttggaaagg gtacagtgaa
     2341 aacatgagga agctcagaaa gactcatgat attccatgcc cagatcttcc ttggagctat
     2401 aaaatagaat aatttttctt tcaagtaggt tagctagctc tcccccaata aaaataccac
     2461 tatcagagta gaaacaaggt agacatttct aaaacatatg tgcttcatta tgaatccatg
     2521 gatggctcaa ttcttaaatg taaatagatc tctaggaaac tcaaagtaca gtgttttcaa
     2581 tctaaaaaga agtattggca gtttcacttg caaaattaca cagctggtcc ctgtgatgtg
     2641 tctcgacacc aatacacaac ccccttccca ccatccttca tgtcactaga tacacaaccc
     2701 ccttcccacc atcccttcag tcactagtgg aagctttcaa gttagttatt tcagtcagga
     2761 tatacagtgt tgaaatctca atgcagttga aatctggtct gatgtgccta atttatctgt
     2821 ggaaaattta atgctgaatt acatttggtt gggaaatgtc cctcaaaatc ctgggcacta
     2881 tgaaggaacc ccctgccccc tcaccttttt gggtaggtaa aagactaaaa gccatatgga
     2941 ttttaactga taacaatgaa agtggtaaat cagtgtaaaa gtgtcatatt ctcagacttg
     3001 tgaggcggtt tatagtcaga aagatttacg gatttttttc ctgtaacata aaagattgtg
     3061 aacttttttt aattaaaaaa tatttcctag ggctgtagtt atttgggagt ttcataacct
     3121 gttatggtgc ttttggtgga aatttttatt atttagcatt ttaggagacc gctgtcaact
     3181 ggttttaatc tatgatgcta atgtgttttc cactgtaccc tcatctcagg aataaaactg
     3241 ctttaacgga gatgatgtca ggtacaaata cactatagag tcaaaatacc atttacaaag
     3301 aaaatcaaaa gcatttctat attttgtctt tttttagttc agacagcaaa ggcatgtact
     3361 actataaaat acaaagtgat tttagagaat gaaaaatgct acttttatct tctctaaaat
     3421 tatttccccc aaggtagtga agtaattgga atgaaggagg ctgaaagtat tgtctaaagt
     3481 gagcccagag gccactgaga atgcagatta ctgacagcca ggtctgttta gttgtaattg
     3541 gaagacacat gagtgtcctg cttacatgta gcttcagact gcagagacag gacgtgtgct
     3601 tttcatttca atatttagtt atatttgata ttttgaaact gtctgctttt tgctatttct
     3661 gcagtttcaa gttagttaga agcatgttgt caactaaaga caacaaacta tcagattcat
     3721 tcattcagtg aagcagcctc tgattctcta agagtcacga atgtcttagt gttaccctcc
     3781 cctagtcaac agcagaccag cctggccaat gcctatgggt ggcccctctg gagtgctcac
     3841 taacaagggt gagtgctctc gctaagaagt gtcccccgcc taatcatgtg tttataggat
     3901 agtacacgtt ccccaggccc ataacagagg actaaaatct ctgaatttta aagacacaga
     3961 tgactggcat attttggata ccagtatagc tatatcaaat agacaaaaac agcttcactt
     4021 tagcaatgat cagattgtta atctacagat tttatttttt aaaatttgga tgtaagtaga
     4081 gactttcagt atttgttttc tcttgatttt gaagtcattt cttcttctca cgtctgtgac
     4141 aaatggttga aaaggagtca acatggcccc aactatagtg ccggaacctt ttcatcattc
     4201 tgaggctttg ccccacacat ggtcctcact catatctgtc accttctgaa gcctagatct
     4261 tgttaaccca tcaggtgcag tgtcagtttc aaatcaaatt atctaagaaa acaagaaaac
     4321 aaaggcagca gactattggt acacattata gtccaaagtg cttagcgaag taaaaaaaaa
     4381 agctttttaa aatttctatt gttgtctatt ggtaatgttt ttgatcagaa taaagagggt
     4441 aaagggaaaa agttactaca catgctaggc tttctcagtg gggaaaaaaa tggctggata
     4501 gaactgggac aaacacagac ccatctttag gggtctggat tttgtaggtc cgactacaca
     4561 gcagtgttaa ctcatttctc atgccattag ctctctacaa aataaagcaa agtagttcta
     4621 gtgtggtcgt tataaaccaa tattgtgaaa aatagcaact attcatttgt tcacaacatg
     4681 cgtatttata gagtagttag gtaccatttg taaggtaaat cctttaaaat tctataatac
     4741 atactaaaat agtggttatt ggtctgatat atgctgctct tggttctata aactagataa
     4801 aagcagtgct ttgtgaaatg cagtgttctc tcttaacgcc actggtgata ggaagtagtt
     4861 cccttcagtt caaatcctgt gcccttattt gctgcttgct gacgtaagca ataagtaacc
     4921 ctctaactaa tggtatctac atatttctgt aacttgtatt taatgatggt gtacctggtg
     4981 attgtaaaaa tattagacag atataaaagt atctatataa tatctataaa ctgttaatgc
     5041 tgaggtatag tctgtgaatt atgtgttttg tatttttatt cattgtgtaa tttagtggtg
     5101 gtgaaagttc tacactcaat ccttaaagag tggcagtatc cctttttcaa tttaacatgg
     5161 tctgcatcaa tctgtttgcc tgctaaacaa gttagaatag gaaatagtaa aataaatcca
     5221 aacaagagtg taatattggt tagggaaaca aacttcaggt caccaactga agtcattaca
     5281 aactccttgt acattcactg tgagtttcaa agggagtcag tccctaattt acaggtttcc
     5341 tttgttcact ttctagatgt gtacttttga gggacatgag gttaggcaac attacagcaa
     5401 cacacactgg ggctattaat cccatttagg tctgtactaa agaggatggg tagaaacaca
     5461 tatgtatata ccttttcttt acctagaagt gccatccatt gtccttgaat tattatgatt
     5521 aaagttactg ttgcatttag gaggctcctt gaaagtaaca ccttttccaa ggacaatggc
     5581 aacaatgtca atgtcaacac tgagtctaat tttgaccaca tttatagtgg tatagtgtca
     5641 atactgcatt ttcatgacaa ccacactcca ctgttgaaac actgtgccag tgagaagtgc
     5701 aacatccagt gggcagatga aaatgatgca tggccaagtt cagtgtttac agataaaact
     5761 gctgtggttc aagtgcttcc ttccccatca aatccttgat aaggctttgt gggaaaatga
     5821 tctaaattat tgttttaatt tttaaaccta atttattcac tcagtgaaca ttcttaccta
     5881 agcaaggcta taaaagggaa tactacagtt ttgtccacac tttaatttga gaccattttt
     5941 ctttgaatcg taagttagca aaagaaattt ttttcacctt tgaaaatcac gtgatgttaa
     6001 aggacaaatg cccactcttc tattctgatc tgcaaatagc ttaaatgtct cttgcaaaac
     6061 aaagtaagat accaccagta ttacaacaca atgattttcc agacaaatgg tatggtgttg
     6121 aaaaataact gttacttctt aaagcagcat tttatcttct attttgaaga ctatttattg
     6181 taataattag aaaacatgaa atagggaact ccagactgac acagcagttg tttttgaaaa
     6241 ggaaataaac tttgatgaat tagataatcc agatacatca ttgtaaactc ttactctagg
     6301 tgctctttgg tgagagacag gctttgttct cttgttcata acatttctct gcaaagaatt
     6361 ctctatggag tgaagcgaat gaagtgaata atttcttacc aaataaattt atcaatttac
     6421 aaatctgctc tacatctcac ttttgagttc agttgtagtc atgagtgatc cttcatattt
     6481 tattaaaagt gttcattcag gtaaggtgta tgttgaaatt ttgccaatta tcttaataaa
     6541 acctggcaat ttaaaaacca
//
