LOCUS       NM_021003               8231 bp    mRNA    linear   PRI 06-AUG-2024
DEFINITION  Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1A (PPM1A),
            transcript variant 1, mRNA.
ACCESSION   NM_021003
VERSION     NM_021003.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Cheng,P., Wang,Y., Wu,Q., Zhang,H., Fang,W. and Feng,F.
  TITLE     Role of TRIM59 in regulating PPM1A in the pathogenesis of silicosis
            and the intervention effect of tanshinone IIA
  JOURNAL   Biomed Pharmacother 177, 117014 (2024)
   PUBMED   38908195
  REMARK    GeneRIF: Role of TRIM59 in regulating PPM1A in the pathogenesis of
            silicosis and the intervention effect of tanshinone IIA.
REFERENCE   2  (bases 1 to 8231)
  AUTHORS   Weon,S., Jo,S., Nam,B., Choi,S.H., Park,Y.S., Kim,Y.G. and Kim,T.H.
  TITLE     Extracellular PPM1A promotes mineralization of osteoblasts
            differentiation in ankylosing spondylitis via the FOXO1A-RUNX2
            pathway
  JOURNAL   J Cell Mol Med 27 (5), 650-658 (2023)
   PUBMED   36756789
  REMARK    GeneRIF: Extracellular PPM1A promotes mineralization of osteoblasts
            differentiation in ankylosing spondylitis via the FOXO1A-RUNX2
            pathway.
REFERENCE   3  (bases 1 to 8231)
  AUTHORS   Feng,D., Zhou,J., Liu,H., Wu,X., Li,F., Zhao,J., Zhang,Y., Wang,L.,
            Chao,M., Wang,Q., Qin,H., Ge,S., Liu,Q., Zhang,J. and Qu,Y.
  TITLE     Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier
            disruption after subarachnoid hemorrhage
  JOURNAL   Sci Adv 8 (39), eabq2423 (2022)
   PUBMED   36179025
  REMARK    GeneRIF: Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain
            barrier disruption after subarachnoid hemorrhage.
REFERENCE   4  (bases 1 to 8231)
  AUTHORS   Liu,C., Sun,W., Yang,K. and Xia,B.
  TITLE     Knockdown of TRIM65 suppressed the proliferation and invasiveness
            of gastric cancer cells by restricting the ubiquitin degradation of
            PPM1A
  JOURNAL   Exp Cell Res 416 (2), 113154 (2022)
   PUBMED   35421368
  REMARK    GeneRIF: Knockdown of TRIM65 suppressed the proliferation and
            invasiveness of gastric cancer cells by restricting the ubiquitin
            degradation of PPM1A.
REFERENCE   5  (bases 1 to 8231)
  AUTHORS   Li,M., Xu,X., Su,Y., Shao,X., Zhou,Y. and Yan,J.
  TITLE     A comprehensive overview of PPM1A: From structure to disease
  JOURNAL   Exp Biol Med (Maywood) 247 (6), 453-461 (2022)
   PUBMED   34861123
  REMARK    GeneRIF: A comprehensive overview of PPM1A: From structure to
            disease.
            Review article
REFERENCE   6  (bases 1 to 8231)
  AUTHORS   Fjeld,C.C. and Denu,J.M.
  TITLE     Kinetic analysis of human serine/threonine protein phosphatase
            2Calpha
  JOURNAL   J Biol Chem 274 (29), 20336-20343 (1999)
   PUBMED   10400656
REFERENCE   7  (bases 1 to 8231)
  AUTHORS   Takekawa,M., Maeda,T. and Saito,H.
  TITLE     Protein phosphatase 2Calpha inhibits the human stress-responsive
            p38 and JNK MAPK pathways
  JOURNAL   EMBO J 17 (16), 4744-4752 (1998)
   PUBMED   9707433
REFERENCE   8  (bases 1 to 8231)
  AUTHORS   Das,A.K., Helps,N.R., Cohen,P.T. and Barford,D.
  TITLE     Crystal structure of the protein serine/threonine phosphatase 2C at
            2.0 A resolution
  JOURNAL   EMBO J 15 (24), 6798-6809 (1996)
   PUBMED   9003755
REFERENCE   9  (bases 1 to 8231)
  AUTHORS   Redpath,N.T., Price,N.T., Severinov,K.V. and Proud,C.G.
  TITLE     Regulation of elongation factor-2 by multisite phosphorylation
  JOURNAL   Eur J Biochem 213 (2), 689-699 (1993)
   PUBMED   8386634
REFERENCE   10 (bases 1 to 8231)
  AUTHORS   Mann,D.J., Campbell,D.G., McGowan,C.H. and Cohen,P.T.
  TITLE     Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and
            comparative analysis of amino acid sequences
  JOURNAL   Biochim Biophys Acta 1130 (1), 100-104 (1992)
   PUBMED   1311954
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL157756.3, DA439296.1,
            BC063243.1, AL132778.6 and AI076736.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 24, 2018 this sequence version replaced NM_021003.4.
            
            Summary: The protein encoded by this gene is a member of the PP2C
            family of Ser/Thr protein phosphatases. PP2C family members are
            known to be negative regulators of cell stress response pathways.
            This phosphatase dephosphorylates, and negatively regulates the
            activities of, MAP kinases and MAP kinase kinases. It has been
            shown to inhibit the activation of p38 and JNK kinase cascades
            induced by environmental stresses. This phosphatase can also
            dephosphorylate cyclin-dependent kinases, and thus may be involved
            in cell cycle control. Overexpression of this phosphatase is
            reported to activate the expression of the tumor suppressor gene
            TP53/p53, which leads to G2/M cell cycle arrest and apoptosis.
            Three alternatively spliced transcript variants encoding distinct
            isoforms have been described. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) is the longest transcript and
            encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.219432.1,
                                           SRR1803614.109713.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000395076.9/ ENSP00000378514.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                AL157756.3         44091-44117         c
            28-244              DA439296.1         1-217
            245-2495            BC063243.1         1-2251
            2496-8229           AL132778.6         7331-13064
            8230-8231           AI076736.1         7-8                 c
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q23.1"
     gene            1..8231
                     /gene="PPM1A"
                     /gene_synonym="PP2C-ALPHA; PP2CA; PP2Calpha"
                     /note="protein phosphatase, Mg2+/Mn2+ dependent 1A"
                     /db_xref="GeneID:5494"
                     /db_xref="HGNC:HGNC:9275"
                     /db_xref="MIM:606108"
     exon            1..457
                     /gene="PPM1A"
                     /gene_synonym="PP2C-ALPHA; PP2CA; PP2Calpha"
                     /inference="alignment:Splign:2.1.0"
     exon            458..1311
                     /gene="PPM1A"
                     /gene_synonym="PP2C-ALPHA; PP2CA; PP2Calpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             478..1626
                     /gene="PPM1A"
                     /gene_synonym="PP2C-ALPHA; PP2CA; PP2Calpha"
                     /EC_number="3.1.3.16"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein phosphatase 1A"
                     /codon_start=1
                     /product="protein phosphatase 1A isoform 1"
                     /protein_id="NP_066283.1"
                     /db_xref="CCDS:CCDS9744.1"
                     /db_xref="GeneID:5494"
                     /db_xref="HGNC:HGNC:9275"
                     /db_xref="MIM:606108"
                     /translation="MGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVI
                     GLPSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIR
                     TGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFF
                     TQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPE
                     PEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCL
                     YKGSRDNMSVILICFPNAPKVSPEAVKKEAELDKYLECRVEEIIKKQGEGVPDLVHVM
                     RTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW"
     exon            1312..1429
                     /gene="PPM1A"
                     /gene_synonym="PP2C-ALPHA; PP2CA; PP2Calpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1538
                     /gene="PPM1A"
                     /gene_synonym="PP2C-ALPHA; PP2CA; PP2Calpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1539..1596
                     /gene="PPM1A"
                     /gene_synonym="PP2C-ALPHA; PP2CA; PP2Calpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..8231
                     /gene="PPM1A"
                     /gene_synonym="PP2C-ALPHA; PP2CA; PP2Calpha"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2472..2477
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPM1A"
                     /gene_synonym="PP2C-ALPHA; PP2CA; PP2Calpha"
                     /note="hexamer: AATAAA"
     polyA_site      2499
                     /gene="PPM1A"
                     /gene_synonym="PP2C-ALPHA; PP2CA; PP2Calpha"
                     /note="major polyA site"
     regulatory      8211..8216
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPM1A"
                     /gene_synonym="PP2C-ALPHA; PP2CA; PP2Calpha"
                     /note="hexamer: AATAAA"
     polyA_site      8231
                     /gene="PPM1A"
                     /gene_synonym="PP2C-ALPHA; PP2CA; PP2Calpha"
                     /note="major polyA site"
ORIGIN      
        1 agaggcggcg gcggcggcgg tggcggcgct agggacggga gcgcgcgcgg gagctagaga
       61 gcagtggtct cggcgctcgt ccggcccgca gcttcgggtc ctcaggcggc tgttgctccg
      121 gaacgggtgg ttggggaggg gggggtgggg ggactctaga cagctgaggc gcgaaagcga
      181 tgagtcctcg gctcttcctc ctccttctcc gggacccgct ctctgcctcc ctctccaacg
      241 cccggatgat ctgagccgcg agggcgccga cagccggggg cccggacgca gcccggctcc
      301 tcccctcctc cgccccttcc ccagcctgac ctggcccgcc gctgcagcgg tgacccctcc
      361 cccggctgcc gccgtcgccg ccgcggtgac cccctccccg gctgccgccg ccgccgcctc
      421 ggccgaccag ggacctgccc gcctgcggct gctccggacc tagaggatca agacataatg
      481 ggagcatttt tagacaagcc aaagatggaa aagcataatg cccaggggca gggtaatggg
      541 ttgcgatatg ggctaagcag catgcaaggc tggcgtgttg aaatggagga tgcacatacg
      601 gctgtgatcg gtttgccaag tggacttgaa tcgtggtcat tctttgctgt gtatgatggg
      661 catgctggtt ctcaggttgc caaatactgc tgtgagcatt tgttagatca catcaccaat
      721 aaccaggatt ttaaagggtc tgcaggagca ccttctgtgg aaaatgtaaa gaatggaatc
      781 agaacaggtt ttctggagat tgatgaacac atgagagtta tgtcagagaa gaaacatggt
      841 gcagatagaa gtgggtcaac agctgtaggt gtcttaattt ctccccaaca tacttatttc
      901 attaactgtg gagactcaag aggtttactt tgtaggaaca ggaaagttca tttcttcaca
      961 caagatcaca aaccaagtaa tccgctggag aaagaacgaa ttcagaatgc aggtggctct
     1021 gtaatgattc agcgtgtgaa tggctctctg gctgtatcga gggcccttgg ggattttgat
     1081 tacaaatgtg tccatggaaa aggtcctact gagcagcttg tctcaccaga gcctgaagtc
     1141 catgatattg aaagatctga agaagatgat cagttcatta tccttgcatg tgatggtatc
     1201 tgggatgtta tgggaaatga agagctctgt gattttgtaa gatccagact tgaagtcact
     1261 gatgaccttg agaaagtttg caatgaagta gtcgacacct gtttgtataa gggaagtcga
     1321 gacaacatga gtgtgatttt gatctgtttt ccaaatgcac ccaaagtatc gccagaagca
     1381 gtgaagaagg aggcagagtt ggacaagtac ctggaatgca gagtagaaga aatcataaag
     1441 aagcaggggg aaggcgtccc cgacttagtc catgtgatgc gcacattagc gagtgagaac
     1501 atccccagcc tcccaccagg gggtgaattg gcaagcaaga ggaatgttat tgaagccgtt
     1561 tacaatagac tgaatcctta caaaaatgac gacactgact ctacatcaac agatgatatg
     1621 tggtaaaact gctcatctag ccatggagtt taccttcacc tccaaaggag agtacagctc
     1681 aactttgttg aaacttttaa catccatcct caactttaag gaaggggata tgacatgggt
     1741 gagaatgatt acatcagaga acttcagcag tacaacagct agcccagaac tgattttttt
     1801 tttttttttt gtaaatttga gacttatgta agcgtgattt caaaccataa ttcgtgttgt
     1861 aaatcagact ccagcaattt ttgttgtatg attttgtttt tttgtaaagt gtaattgtcc
     1921 ttgtacaaaa tgctcatatt taattatgaa ctgctttaaa tcactatcaa agttacaaga
     1981 aatgtttggc ttattgtgtg atgcaacaga tatatagccc tttcaagtca tgttgtgttt
     2041 ggacttgggg ttggaacagg gagagcagca gccatgtcag ctacacgctc aaatgtgcag
     2101 atgattatgg aaaataacct caaaatctta caaagctgaa catccaagga gttattgaaa
     2161 actatcttaa atgttcttgg taggggagtt ggcattgttg ataaagccag tcccttcatt
     2221 taactgtctt tcaggatgtt ccttcgttgt ttccatgagt attgcaggta ataatacagt
     2281 gtattcataa gaatctcaat cttggggcta aatgccttgt ttctttgcac ctcttttcaa
     2341 gtccttacat ttaattacta attgataagc agcagcttcc tacatatagt aggaaactgc
     2401 cacatttttg ctatcatgat tggctgggcc tgctgctgtt cctagtaaga tattctgaat
     2461 tccattttat caataaagct tgatttaaca aacaagaaac ttaatcatgt atgtgtaatt
     2521 cctcttttac cctggccttt taaaacactg tgccgttgta atgagacgtt tctcataggg
     2581 aaagatgtta gtctctttta attggacaac actgtcactc aaggcataga tgaaactttc
     2641 cttccattag aaagactaaa agatttaatt cttggttgta ccttaatcta ttttttaaat
     2701 aggtttcttt caggctgctt atttttcatt aagatgtgta tcagcttgga tttgcctact
     2761 gtttaattaa aatatttatt gtcaaagttt gacaatctaa cactctatgg taggggtgtg
     2821 tgtgtgtgtg tttgtgagtg tgtgtttgcc tgtgattttt aattggccca tgtctttaga
     2881 atccaagtgg ttaagatgta tttgtgattt gaaatatagc atgttgataa tatttagctg
     2941 ttggccttta caaataactt tcaaagctta aggaattgta gatataaaaa taacctaatt
     3001 taatttaggc ttaaattcct ctgattaagc atgtgaaagt aagttttaaa atctgtcgca
     3061 ttgaaaagat tactgttccg tgcccttctg tatttttgtc tctttaggtt gaatattgta
     3121 tttatcacca tgtaatcatt cagtaggcag attcccacta gaaaactgtt gaaatgtaag
     3181 actaaaatac aacattgaat acaaaatcaa aattttgtgt ataaaaacca gtatagtcca
     3241 ttttgttata tttgtttttt ccctaacttg gaaatataca tatttgtata tatagcctta
     3301 aaattaatgt aaagttaggg gagtagtggg gaaagtaatg tgaaatgtct cagatttaag
     3361 tagttaaata ccagcaaaat cttttcatta tccctcttat tttgtgaggt gattaaatgt
     3421 aacttaattg tatttaattt atatcttatt ccagcatgaa tgaggaaaaa ctgaagtact
     3481 atttatattt agaaattcat atcagttgaa attacagaac caattccata cttacaataa
     3541 atacttaatg tctaaatctg tggtagagtg cgaagtatga taatgttcta agttatggct
     3601 ttgcaagcat ctaaatgtgc atttaatgaa taccagtgct tctagtatag actaattacc
     3661 agacatactg gtactgaaag ctaaatccct attataacaa accagttcct taatatttta
     3721 agtagactga caactttagt tccagaaatt gcaaaacttt gaactggact gtgtaatctt
     3781 ttgagatgca aaacttaagt cacaagtaga gtatgtgatg gaaagctgta tttcaaacca
     3841 taacagcata tttagagcct tttttttttg agtctttaaa caagagaaaa ttaaaatatt
     3901 cctgtcaaaa ttattagtat tgaaattagg cttggacacg agagagaacc gtatttgagt
     3961 gatgtgagaa gactaaatct tttccacatg agtcagcact gccatactaa taattttttt
     4021 actataaaaa tacaggaagg aagtatacat tataacagca gactgtgtgt gttcctgatt
     4081 cctggaggta atagtggggg gaaaccaacc atacttttta aaggcacttt tgcacctcta
     4141 ttgtgcactt cattcttgta ccacttaaat tcttcacccc catccccttt ttttgtgcta
     4201 attagcatct cagggcaatg cctcaaaaat gtttgatgtg ttctgttctt tggagggaaa
     4261 aagttcttat gtgatgataa tatagtactc aaaatatact tttatcattt aaatgtctta
     4321 tttgctgcta tgaataggaa ataacatttt gtatagcagg ctctgtttta ccctaacatt
     4381 aaaaaatttc actgatcttt ctttcattaa cagggtagaa tctcctaatt tccactttct
     4441 tgggaatata cttttataga caatagaagc agttctcaat attagcatat actttaaaaa
     4501 atcaaagtga taacttaatt cagctttgga agtatctcaa acatattttt actttatagt
     4561 gcattaactt gcttctagag tacttaatgc aactgctcta gccacttaat tttttatact
     4621 aatctcaaca ttaagaaatt tggattaagt aataaattag ttatgtaatt caagtaatct
     4681 gaattacagc agtactttta gtgatcattc ataggactat atattaaccc agctaataac
     4741 tcagtttttt tacaaaatgt ttcgagtatt attggtaaaa cactgttcta ggctaagcac
     4801 attgggactg taaagaaatg agtagatcct tggcttcaag tttacatctg gacaatttat
     4861 aatctagtgt atgttagtat tataactgga tcactcatca aaaaatatat atatatatct
     4921 attgcccacc tgctatctac caggtactta gctgatcaag gcaggcccct gccctaaaga
     4981 ccttgtttat acttccttta ctcacctgaa aactgttctc cagtttattt tcttctctct
     5041 aaagttaaag agtaattcag aagaaaattt tgcttagcat aagaataaaa ttggactgaa
     5101 gaggcttaag cccattcagt atccttgatt gcatttatcc aacggccttt attcttcctg
     5161 ctgacagcag taactcagag gaataggtag tagatttctg aaaattatcc agccatggaa
     5221 atgtaggtgg ggtttgagtt taaggcattt aaaaatgtaa atatctctag ctaaatttat
     5281 cttaagtaga actctgtgtt tttgtaacac actgccagtg ttaatatcaa attttagcca
     5341 aattattact atgtgtttta atattttaaa ataatttcac tgcccatctt tactggacaa
     5401 actcatttgg agttcaactt gtgatttctg aaagaactga tgaaattggg tactgctttt
     5461 tttctccatt tttcgttttg ttttaatttt gaatttcatg gtatatactt ttagttcaaa
     5521 ctcagctgtt tgtacagtat tgtattagga tttggtatta gaaaagatgt gtaaatatct
     5581 tagtatataa ttgtttctca tttgaggttt ttcttctaag ggaccttaaa gagttttata
     5641 tacttttgct cacagaaact gctggtgaga ttaccatttt ttgagtatct agtcttctag
     5701 tttttctttt aggcattagg aagccttctt tagagttcaa aattttagaa gcctaatttg
     5761 ctcttacttc cttcaattat gtgccatgtg ttttggtttg tatatgtttt aaattgtata
     5821 tttccttgga atatgcttga aatatttaag aatacatttt caaaatgtat aatactgtat
     5881 tgttttgttg atcagaataa taagtctcag ttaaatgttt gttattactg atagtcaaaa
     5941 tgctcaatag aaatgatgag aggcattggt tccaattcat tgtcaaatga acgttttcta
     6001 attttgttca cagattcttt ccctttcgat tgttctgtat gttaagatag tggcttctgc
     6061 tctcactgtt ttcctattta tattactagc aggtaggagt gctaattaga aaaacttaga
     6121 tggtattgaa attacagttg acaacttata tttttatgag atggagaaaa aagattaagt
     6181 tgatataaca acaaagtgga ctttttttct tccttatcct gcacgaaata ttgcccttgt
     6241 ttcctctact ttcctcttgg tgttttctct ttttttcaaa cagaaacagg ccaattccat
     6301 tttcttgagc aagaaagctt agtgtgttac ttcatcaagg ccagctaata ctgtgttaaa
     6361 ccgggctgaa aatgagaaaa cttgggagat ggaggaatgg ggaaatggca gtgggatagg
     6421 tagggaagga ttactcttaa ttgttttaaa agccatagga aagtcttcct tgtacgtggc
     6481 tgtaaattta taagaactat tgtgtcacat aaaccaacaa gaatgaacct ttgctgcttc
     6541 agataatttg atttttccag caaggaaatt aataagttac tgattcttca gcatagaaac
     6601 aactgagaag aattaatgca atgtttcttc actagaaaac ccaacccttc atttcttttc
     6661 attgctccaa aacccagttt tcaactaatg gttttctcat taaactaaat gtttagaaaa
     6721 gttgtttaga gtttttcttt ttcttttaca tagtcctcct gatccagtat aagactattt
     6781 agtaacgtgc atttgtatgg tactatctaa agtaagttag attgatgtaa gagatcgggt
     6841 agctgcggaa caaaattagt tatatcctaa ttaggtacag tgaatgacac aaaatcattt
     6901 tagcaatgct tcttaacctt ttggggtcac aggcgttttg agactgatga atcctaggga
     6961 cttatttacc caggaaaatg cgtatataac atacatatct ccctaaagtt tacaatattg
     7021 tagtggttca tgggccccct ggttaagagc ccattctaaa gtacaatagg gcatcatccc
     7081 ttttcctgca aagcccaaaa gtatatttct agggcatgaa aataacttga gtctatttta
     7141 aggaattgtt tcactctaga ggtagatagg ggacctggct agaatctgac attaaaatat
     7201 actttttaaa aaatattata tttggggtgg ggaaagtgat taaaaggtga aaaaaaaaca
     7261 tagtattcag aagttttgga ggttaatgtc tttctctaag atttgccact ttagaaattc
     7321 aacagaaaag aggtaaaaca gaaatggaat gtatctggaa catttttggc ctccatagtg
     7381 cagatatact atattaacaa gtaatacatt tatttacctg tcagatctcc aggttttaag
     7441 attttgagct ttctagtatt aggattcatt aaatgttcaa ttcatttcat attctaagga
     7501 attaggttat ttacttacta attcaggatg ttaaaataac atccaagtcg gacaaccacc
     7561 accaatgcac acagttaatg agatttctaa aatataataa gtacaatgta acaaacgtat
     7621 agaattttgc atttgttgcc aaaattagat gtttaatgac agcttattta attcccattt
     7681 gtgggacttc tggaacatag aaaccattat cttacctggt tatcccttga ctaaatagca
     7741 tatctgcagg aaaatatctt gtttgtagtg atatgcccca atagtgattg atttcactct
     7801 tgaaatgagt tatatcactt aatttgtata aatgttatga gtggagagac atgtacatgt
     7861 taaaagcatg ttgcattata tattcatttt ttaaactcta taaatgttaa gaataatata
     7921 attgcagaaa tatttttctt aaatacaatg tgtaacaaaa ttctccgtag caactcaccc
     7981 actttgcagt ttatgtgatc cacactttta aagaaattcc ataaatgtat attttgtatt
     8041 atgtattatt tcctggtcca aagaaaatat gtgaattcag ttctaacttt aagaatgtac
     8101 tgtttgtttt caagttcatt gaaaaattgc attcagcctg cgaatggttg cagattgtat
     8161 gttagatgaa aagtagaaat aatttctagt ttggaaaact ggtgccacta aataaacagg
     8221 caattacata a
//
