LOCUS       NM_002705               6251 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens periplakin (PPL), mRNA.
ACCESSION   NM_002705
VERSION     NM_002705.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Shoda,T., Kaufman,K.M., Wen,T., Caldwell,J.M., Osswald,G.A.,
            Purnima,P., Zimmermann,N., Collins,M.H., Rehn,K., Foote,H.,
            Eby,M.D., Zhang,W., Ben-Baruch Morgenstern,N., Ballaban,A.Y.,
            Habel,J.E., Kottyan,L.C., Abonia,J.P., Mukkada,V.A., Putnam,P.E.,
            Martin,L.J. and Rothenberg,M.E.
  TITLE     Desmoplakin and periplakin genetically and functionally contribute
            to eosinophilic esophagitis
  JOURNAL   Nat Commun 12 (1), 6795 (2021)
   PUBMED   34815391
  REMARK    GeneRIF: Desmoplakin and periplakin genetically and functionally
            contribute to eosinophilic esophagitis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 6251)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 6251)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 6251)
  AUTHORS   Odintsova,E., Mohammed,F., Trieber,C., Rodriguez-Zamora,P.,
            Al-Jassar,C., Huang,T.H., Fogl,C., Knowles,T., Sridhar,P.,
            Kumar,J., Jeeves,M., Chidgey,M. and Overduin,M.
  TITLE     Binding of the periplakin linker requires vimentin acidic residues
            D176 and E187
  JOURNAL   Commun Biol 3 (1), 83 (2020)
   PUBMED   32081916
  REMARK    GeneRIF: Binding of the periplakin linker requires vimentin acidic
            residues D176 and E187.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6251)
  AUTHORS   Amagai,M.
  TITLE     Autoimmunity against desmosomal cadherins in pemphigus
  JOURNAL   J Dermatol Sci 20 (2), 92-102 (1999)
   PUBMED   10379702
  REMARK    Review article
REFERENCE   7  (bases 1 to 6251)
  AUTHORS   Aho,S., Rothenberger,K., Tan,E.M., Ryoo,Y.W., Cho,B.H., McLean,W.H.
            and Uitto,J.
  TITLE     Human periplakin: genomic organization in a clonally unstable
            region of chromosome 16p with an abundance of repetitive sequence
            elements
  JOURNAL   Genomics 56 (2), 160-168 (1999)
   PUBMED   10051401
REFERENCE   8  (bases 1 to 6251)
  AUTHORS   Ruhrberg,C., Williamson,J.A., Maatta,A. and Watt,F.M.
  TITLE     The periplakin gene maps to 16p13.3 in human and 16A-B1 in mouse
  JOURNAL   Genomics 49 (1), 157-159 (1998)
   PUBMED   9570964
REFERENCE   9  (bases 1 to 6251)
  AUTHORS   Aho,S., McLean,W.H., Li,K. and Uitto,J.
  TITLE     cDNA cloning, mRNA expression, and chromosomal mapping of human and
            mouse periplakin genes
  JOURNAL   Genomics 48 (2), 242-247 (1998)
   PUBMED   9521878
REFERENCE   10 (bases 1 to 6251)
  AUTHORS   Ruhrberg,C., Hajibagheri,M.A., Parry,D.A. and Watt,F.M.
  TITLE     Periplakin, a novel component of cornified envelopes and desmosomes
            that belongs to the plakin family and forms complexes with
            envoplakin
  JOURNAL   J Cell Biol 139 (7), 1835-1849 (1997)
   PUBMED   9412476
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC027687.7.
            
            On Nov 24, 2018 this sequence version replaced NM_002705.4.
            
            Summary: The protein encoded by this gene is a component of
            desmosomes and of the epidermal cornified envelope in
            keratinocytes. The N-terminal domain of this protein interacts with
            the plasma membrane and its C-terminus interacts with intermediate
            filaments. Through its rod domain, this protein forms complexes
            with envoplakin. This protein may serve as a link between the
            cornified envelope and desmosomes as well as intermediate
            filaments. AKT1/PKB, a protein kinase mediating a variety of cell
            growth and survival signaling processes, is reported to interact
            with this protein, suggesting a possible role for this protein as a
            localization signal in AKT1-mediated signaling. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF001691.1, AF013717.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000345988.7/ ENSP00000340510.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-165               AC027687.7         129565-129729       c
            166-265             AC027687.7         103431-103530       c
            266-420             AC027687.7         96467-96621         c
            421-541             AC027687.7         94987-95107         c
            542-667             AC027687.7         93545-93670         c
            668-709             AC027687.7         93411-93452         c
            710-871             AC027687.7         91804-91965         c
            872-979             AC027687.7         91594-91701         c
            980-1075            AC027687.7         90256-90351         c
            1076-1198           AC027687.7         88175-88297         c
            1199-1345           AC027687.7         87842-87988         c
            1346-1497           AC027687.7         87048-87199         c
            1498-1595           AC027687.7         86122-86219         c
            1596-1753           AC027687.7         85794-85951         c
            1754-1932           AC027687.7         84616-84794         c
            1933-2071           AC027687.7         84392-84530         c
            2072-2265           AC027687.7         83309-83502         c
            2266-2416           AC027687.7         82765-82915         c
            2417-2500           AC027687.7         81559-81642         c
            2501-2617           AC027687.7         80683-80799         c
            2618-2710           AC027687.7         79716-79808         c
            2711-6251           AC027687.7         75088-78628         c
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..6251
                     /gene="PPL"
                     /note="periplakin"
                     /db_xref="GeneID:5493"
                     /db_xref="HGNC:HGNC:9273"
                     /db_xref="MIM:602871"
     exon            1..165
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     CDS             104..5374
                     /gene="PPL"
                     /note="190 kDa paraneoplastic pemphigus antigen; 195 kDa
                     cornified envelope precursor protein"
                     /codon_start=1
                     /product="periplakin"
                     /protein_id="NP_002696.4"
                     /db_xref="CCDS:CCDS10526.1"
                     /db_xref="GeneID:5493"
                     /db_xref="HGNC:HGNC:9273"
                     /db_xref="MIM:602871"
                     /translation="MNSLFRKRNKGKYSPTVQTRSISNKELSELIEQLQKNADQVEKN
                     IVDTEAKMQSDLARLQEGRQPEHRDVTLQKVLDSEKLLYVLEADAAIAKHMKHPQGDM
                     IAEDIRQLKERVTNLRGKHKQIYRLAVKEVDPQVNWAALVEEKLDKLNNQSFGTDLPL
                     VDHQVEEHNIFHNEVKAIGPHLAKDGDKEQNSELRAKYQKLLAASQARQQHLSSLQDY
                     MQRCTNELYWLDQQAKGRMQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHSE
                     GDQLLAAEHPGRNSIEAHMEAVHADWKEYLNLLICEESHLKYMEDYHQFHEDVKDAQE
                     LLRKVDSDLNQKYGPDFKDRYQIELLLRELDDQEKVLDKYEDVVQGLQKRGQQVVPLK
                     YRRETPLKPIPVEALCDFEGEQGLISRGYSYTLQKNNGESWELMDSAGNKLIAPAVCF
                     VIPPTDPEALALADSLGSQYRSVRQKAAGSKRTLQQRYEVLKTENPGDASDLQGRQLL
                     AGLDKVASDLDRQEKAITGILRPPLEQGRAVQDSAERAKDLKNITNELLRIEPEKTRS
                     TAEGEAFIQALPGSGTTPLLRTRVEDTNRKYEHLLQLLDLAQEKVDVANRLEKSLQQS
                     WELLATHENHLNQDDTVPESSRVLDSKGQELAAMACELQAQKSLLGEVEQNLQAAKQC
                     SSTLASRFQEHCPDLERQEAEVHKLGQRFNNLRQQVERRAQSLQSAKAAYEHFHRGHD
                     HVLQFLVSIPSYEPQETDSLSQMETKLKNQKNLLDEIASREQEVQKICANSQQYQQAV
                     KDYELEAEKLRSLLDLENGRRSHVSKRARLQSPATKVKEEEAALAAKFTEVYAINRQR
                     LQNLEFALNLLRQQPEVEVTHETLQRNRPDSGVEEAWKIRKELDEETERRRQLENEVK
                     STQEEIWTLRNQGPQESVVRKEVLKKVPDPVLEESFQQLQRTLAEEQHKNQLLQEELE
                     ALQLQLRALEQETRDGGQEYVVKEVLRIEPDRAQADEVLQLREELEALRRQKGAREAE
                     VLLLQQRVAALAEEKSRAQEKVTEKEVVKLQNDPQLEAEYQQLQEDHQRQDQLREKQE
                     EELSFLQDKLKRLEKERAMAEGKITVKEVLKVEKDAATEREVSDLTRQYEDEAAKARA
                     SQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQERKYRG
                     AEEQLRSYQSELEALRRRGPQVEVKEVTKEVIKYKTDPEMEKELQRLREEIVDKTRLI
                     ERCDLEIYQLKKEIQALKDTKPQVQTKEVVQEILQFQEDPQTKEEVASLRAKLSEEQK
                     KQVDLERERASQEEQIARKEEELSRVKERVVQQEVVRYEEEPGLRAEASAFAESIDVE
                     LRQIDKLRAELRRLQRRRTELERQLEELERERQARREAEREVQRLQQRLAALEQEEAE
                     AREKVTHTQKVVLQQDPQQAREHALLRLQLEEEQHRRQLLEGELETLRRKLAALEKAE
                     VKEKVVLSESVQVEKGDTEQEIQRLKSSLEEESRSKRELDVEVSRLEARLSELEFHNS
                     KSSKELDFLREENHKLQLERQNLQLETRRLQSEINMAATETRDLRNMTVADSGTNHDS
                     RLWSLERELDDLKRLSKDKDLEIDELQKRLGSVAVKREQRENHLRRSIVVIHPDTGRE
                     LSPEEAHRAGLIDWNMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEEALQ
                     SGRLTPAQYDRYVNKDMSIQELAVLVSGQK"
     exon            166..265
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            266..420
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            421..541
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            542..667
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            668..709
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            710..871
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            872..979
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1075
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1198
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1345
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1497
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1498..1595
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1596..1753
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..1932
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..2071
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            2072..2265
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            2266..2416
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..2500
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            2501..2617
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            2618..2710
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     exon            2711..6251
                     /gene="PPL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6217..6222
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPL"
                     /note="hexamer: AATAAA"
     polyA_site      6251
                     /gene="PPL"
                     /note="major polyA site"
ORIGIN      
        1 agtccgggct ccccgcgccg ccgctcgctc acctgcgctc ccgcccgccc ctcggtgcgc
       61 gcccggcccg ccagccgccg ccgcccgcac cccgagcgcc accatgaact cgctcttcag
      121 gaagagaaac aaaggcaaat acagccccac tgtgcagacc cggagcatct ctaacaagga
      181 gctctcggag ctgatcgagc agctgcagaa gaatgccgac caggtggaga agaacatcgt
      241 ggacacagag gccaagatgc agagtgacct ggctcggctg caggagggtc ggcagcctga
      301 gcaccgggac gtgaccctgc agaaggtgtt ggactctgag aagctgctct atgtgctaga
      361 ggcggatgcg gccattgcca agcacatgaa gcacccacag ggggacatga tcgccgagga
      421 tatccgccag ctgaaggagc gtgtgaccaa cctgcgcggg aaacacaagc agatctacag
      481 gctggcggtg aaggaagtgg atccacaggt caactgggcg gcactggtgg aggagaagct
      541 ggacaagctg aacaaccaga gctttgggac tgacctgccg ctggtggacc accaagtgga
      601 ggagcataac atcttccaca atgaggtcaa ggccatcggg ccccacctgg ccaaggacgg
      661 ggacaaggag cagaacagcg aactccgggc caagtaccag aaactgctgg cagcatcaca
      721 ggcccggcag cagcacctga gttcgctgca ggactacatg cagcgctgca ccaatgagct
      781 gtactggctg gaccagcagg ccaagggccg catgcagtac gactggagtg accgcaacct
      841 cgactacccc agccgccggc gccagtatga gaatttcatc aaccggaacc tggaggccaa
      901 agaggagaga atcaacaaac tgcacagcga gggcgaccag ctgctggcgg ccgagcaccc
      961 cgggaggaac tccattgagg cgcacatgga ggctgtgcac gcagactgga aggagtacct
     1021 gaacctgctc atctgcgagg agagccacct caagtacatg gaggactacc accagtttca
     1081 cgaagacgtg aaggacgctc aggagctgct gcgcaaggtg gactcggacc tgaaccagaa
     1141 gtatggccct gacttcaagg accggtacca gattgagctg ctgctgcggg agctggatga
     1201 ccaggagaag gtgctggaca agtatgagga cgtggtgcag gggctgcaga agcgaggcca
     1261 gcaggtggtg cccctcaagt accgccggga gactccgctc aagcccatcc ccgtggaggc
     1321 actctgtgac tttgaggggg agcagggcct gatctcgcgg ggctacagct acaccctgca
     1381 gaagaacaac ggggagagct gggagctcat ggacagcgct gggaacaagc tgattgctcc
     1441 ggccgtgtgt tttgtgatcc cccccacaga ccctgaggcc ctggctctgg ctgacagcct
     1501 gggcagccag taccggagcg tgcggcagaa ggcagctggg agcaaacgca cgctgcagca
     1561 gcggtatgag gtgctgaaga ccgagaatcc cggagatgcc tctgacctac aggggcggca
     1621 gctgctggct ggcttggaca aggtggccag cgacctggac cggcaggaga aggccatcac
     1681 agggatcctg cggccaccac tggagcaagg ccgggctgtg caggacagtg ccgagcgggc
     1741 caaggacctc aagaacatca ccaacgagct actgcggatt gaacctgaga agacgcggag
     1801 cacggctgag ggcgaagcct tcatccaggc cctcccaggc agtggcacca cacccctgct
     1861 gaggacccgg gtggaggaca ccaaccggaa atacgagcac ctcctgcagc tgctggactt
     1921 ggcccaggag aaggttgatg tggccaaccg cctggagaag agcctgcagc agagctggga
     1981 gttgctggcc acacacgaga accatctgaa tcaggatgac acagtgcctg agagcagccg
     2041 tgtcctggac agcaaggggc aggagctggc ggccatggcc tgtgagttac aggcccagaa
     2101 gtccctcctg ggtgaggtgg agcagaactt gcaggcggcc aagcagtgct cgagcacact
     2161 ggccagccgc ttccaggagc actgtccgga cctggagcgc caggaggccg aggtgcacaa
     2221 gctgggccag cgtttcaaca acctgcgcca gcaggtggaa cgcagggcgc agagcctaca
     2281 gagcgccaag gcagcctacg agcacttcca ccgcggccat gaccacgtgc tgcagttcct
     2341 agtcagcatc cccagttacg agccccagga gacagacagc ctcagccaga tggagaccaa
     2401 gctgaagaac cagaagaacc tgctagatga gatagcaagt agggagcagg aagtacagaa
     2461 gatctgtgcc aattcccagc agtaccagca agctgtaaag gactatgagt tagaagcaga
     2521 aaaactaagg tctcttctcg acttggagaa tggaaggaga agccacgtga gcaagagagc
     2581 caggctccaa tctcctgcca ccaaagtgaa ggaagaggaa gcagcacttg ccgccaagtt
     2641 cactgaagtt tatgccatca acagacagag gctgcagaat ctggagtttg ctctgaatct
     2701 cctcagacag cagccggaag tagaagtgac ccatgagacc ctgcaaagga ataggccgga
     2761 ctctggagtg gaggaggcgt ggaagatcag gaaggaactg gatgaggaga ctgagcggag
     2821 gcggcagctg gagaacgagg tcaagagcac ccaggaagaa atctggacct tgaggaatca
     2881 ggggcctcag gaatcggtgg tgaggaagga ggtgctcaag aaggtgccgg atcccgtgct
     2941 ggaggagagc ttccagcagc tgcagcggac gctggcagag gagcagcaca agaaccagct
     3001 gctgcaggag gagctggagg cactgcagct gcagctgcgt gccctggagc aggagaccag
     3061 agacgggggg caggagtacg tggtcaagga ggtcctgcgc atcgagcctg acagggccca
     3121 ggcggatgag gtcttgcagc tgcgggagga gctggaggca ctgaggcggc agaagggcgc
     3181 ccgggaggca gaggtgctcc tcctgcagca gcgtgtggcc gccctggctg aagagaagag
     3241 ccgggcgcag gagaaggtca cagagaaaga ggtggtgaaa ctgcagaatg acccccagct
     3301 ggaggcagag taccagcagc tgcaggagga ccaccagcgc caggaccagc tcagggagaa
     3361 gcaggaggag gagctgagct tcctccagga caagctcaag aggctagaga aggagcgggc
     3421 catggccgag ggcaagatca ccgtcaagga ggtgctcaag gtggagaagg acgcggccac
     3481 cgagagggag gtcagcgatc tcacccgcca atatgaggac gaggctgcca aggctcgcgc
     3541 tagccagagg gagaagacgg agctgctccg aaagatatgg gccttggagg aggagaacgc
     3601 caaagtggtg gtgcaggaga aggtgcggga gatcgtgcgg ccagacccca aggcggaaag
     3661 tgaagtggcg aacctccgcc tggagcttgt ggagcaggag cgaaagtacc ggggtgccga
     3721 ggagcagctc cggagctacc agagtgagct ggaggccctc aggaggcgag gcccccaggt
     3781 ggaagtcaaa gaggtgacta aggaagtcat taagtacaag actgaccctg agatggagaa
     3841 ggagcttcag cggctcaggg aggagatcgt ggacaagacc agactgatcg aaaggtgtga
     3901 tttagagatc taccagctga aaaaggaaat ccaggccctg aaagacacca aaccccaggt
     3961 ccagaccaaa gaggtggtcc aggagatcct ccaattccaa gaagaccctc aaaccaagga
     4021 ggaggtggcg tctctgaggg caaagctctc agaggagcag aagaaacaag tggatctgga
     4081 gagggaaaga gcttcccagg aagagcagat cgcccggaaa gaggaggagc tctcgcgggt
     4141 gaaggaaagg gtggtgcagc aggaggtggt caggtatgag gaggagccag gcctgcgggc
     4201 cgaggcgagc gcctttgccg agagcatcga tgtggagctg cggcagattg acaagctgcg
     4261 ggcagagctg cggcggctgc agcgccggcg caccgagctt gagcggcagc tggaggagct
     4321 agagcgcgag cggcaggccc gcagggaggc cgagcgcgag gtacagcggt tgcagcagcg
     4381 gctggcagcg ctggagcagg aagaagctga ggcccgtgag aaggtaaccc atacgcagaa
     4441 ggtggtgctg cagcaggacc cgcagcaggc gcgagagcat gccctgctcc gactccagct
     4501 ggaagaagag cagcaccggc ggcagctcct ggagggggag ctcgagaccc tccggaggaa
     4561 actggctgca ctggagaagg cggaggtcaa ggagaaggtg gtgctctccg agagtgtcca
     4621 ggtggagaag ggcgacaccg agcaagagat ccagaggctc aagagcagcc tggaggagga
     4681 gagccgcagc aagcgcgagc tggacgtcga ggtgagccgg ctggaagcca ggctttcgga
     4741 gctggaattc cataactcca agtcatccaa ggaactagac tttctgaggg aagagaacca
     4801 caaattacag ctggagaggc aaaacctgca gctggagacc cgaaggctcc aatcggaaat
     4861 caacatggca gcgacggaaa cacgagacct gcggaacatg accgtggcgg actctgggac
     4921 caaccatgac tccagactgt ggtccctgga gagggaactg gatgacctca agaggctctc
     4981 caaggacaaa gacctcgaga tcgacgagct gcagaagcgc ctgggctccg tggccgtcaa
     5041 gcgggagcag cgggagaacc acctgcggcg ctccatcgta gtcatccacc ctgacacagg
     5101 ccgcgagctg tccccggagg aagcccaccg tgccgggctc attgactgga acatgttcgt
     5161 gaaactcaga agccaggagt gcgactggga ggagatctca gtgaagggtc ccaatgggga
     5221 gtcctcagtg atacacgaca ggaagtctgg caagaagttc tccatcgaag aggccctgca
     5281 gagtggcagg ctgacccctg ctcagtatga ccgctatgtc aacaaggata tgtccatcca
     5341 ggagctggcg gtcttggtat ctgggcagaa gtaggcacag ctcttgcaac tcttcgtaac
     5401 ggcagacgct ggccctctcc tacgacgcag tgacctcctt ggtgtcgtcc tctcccttgc
     5461 cctggtgcag gccaagcatt ttatacatac cctcgctgcc aggctgctca gagtcccgcc
     5521 cacatatgag acaggcactg gctgaagaca ggcaactctc gtcccaacca tcattcaccc
     5581 tgagtgacgt acttcatcct ccaatgactg gacaactgcg atgccttttc ctcccttttc
     5641 ctgtacactc ccagcaacaa actcctcatg ataactgcac acaatctgaa aaccactgaa
     5701 ggacaagcca accacagcag ccaagcccac tccttgcagc atgggtactg gtggcacacc
     5761 agacagtgac actgccccac aaaggcctgg gcccgtgggg gctgctgcct ggcatgacat
     5821 ctctccagat ttctggctta aaaccaactt tccatccgag aagcctcctc agtagttact
     5881 ctgctcatga gacagatctg ggctccaagc caggaaaggt gaacagaaac cacaagtgtc
     5941 cagccctcgg tgctggagtg gacgttaatt gtcagccacc agactgtccc ggcacctaca
     6001 gagaatgttt cacagttctg gcatttaaat cctttgatag tggattgtgc tgctgttagc
     6061 cttagtttca gtgctttaca agtctcgctt attatctcac tggtatttag gtatacaaaa
     6121 cagttgatta ttcaccacgc caatatctgg gtctctgtat ctcatgtaga acataagaaa
     6181 atgggaacta atagggaaat ttatttatag catgaaaata aacctggtgg ctggagtctg
     6241 cttgtacggt c
//
