LOCUS       NM_001394395            5831 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens diphosphoinositol pentakisphosphate kinase 1
            (PPIP5K1), transcript variant 32, mRNA.
ACCESSION   NM_001394395
VERSION     NM_001394395.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Gu,C., Nguyen,H.N., Hofer,A., Jessen,H.J., Dai,X., Wang,H. and
            Shears,S.B.
  TITLE     The Significance of the Bifunctional Kinase/Phosphatase Activities
            of Diphosphoinositol Pentakisphosphate Kinases (PPIP5Ks) for
            Coupling Inositol Pyrophosphate Cell Signaling to Cellular
            Phosphate Homeostasis
  JOURNAL   J Biol Chem 292 (11), 4544-4555 (2017)
   PUBMED   28126903
  REMARK    GeneRIF: This study characterized kinetic properties of the
            bifunctional inositol pyrophosphate 5-diphosphoinositol
            1,2,3,4,6-pentakisphosphatekinase/inositol pyrophosphate,
            1,5-bisdiphosphoinositol 2,3,4,6-tetrakisphosphate phosphatase
            activities of full-length diphosphoinositol pentakisphosphate
            kinase 1 and 2.
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Shears,S.B., Baughman,B.M., Gu,C., Nair,V.S. and Wang,H.
  TITLE     The significance of the 1-kinase/1-phosphatase activities of the
            PPIP5K family
  JOURNAL   Adv Biol Regul 63, 98-106 (2017)
   PUBMED   27776974
  REMARK    GeneRIF: PPIP5K is a rare example of a single protein that
            catalyzes a kinase/phosphatase futile cycle. (Review)
            Review article
REFERENCE   3  (bases 1 to 5831)
  AUTHORS   Machkalyan,G., Trieu,P., Petrin,D., Hebert,T.E. and Miller,G.J.
  TITLE     PPIP5K1 interacts with the exocyst complex through a C-terminal
            intrinsically disordered domain and regulates cell motility
  JOURNAL   Cell Signal 28 (5), 401-411 (2016)
   PUBMED   26854614
  REMARK    GeneRIF: results suggest that PPIP5K1 might play an important role
            in regulating function of exocyst complex in establishing cellular
            polarity and directional migration of cells
REFERENCE   4  (bases 1 to 5831)
  AUTHORS   He,M., Wu,C., Xu,J., Guo,H., Yang,H., Zhang,X., Sun,J., Yu,D.,
            Zhou,L., Peng,T., He,Y., Gao,Y., Yuan,J., Deng,Q., Dai,X., Tan,A.,
            Feng,Y., Zhang,H., Min,X., Yang,X., Zhu,J., Zhai,K., Chang,J.,
            Qin,X., Tan,W., Hu,Y., Lang,M., Tao,S., Li,Y., Li,Y., Feng,J.,
            Li,D., Kim,S.T., Zhang,S., Zhang,H., Zheng,S.L., Gui,L., Wang,Y.,
            Wei,S., Wang,F., Fang,W., Liang,Y., Zhai,Y., Chen,W., Miao,X.,
            Zhou,G., Hu,F.B., Lin,D., Mo,Z. and Wu,T.
  TITLE     A genome wide association study of genetic loci that influence
            tumour biomarkers cancer antigen 19-9, carcinoembryonic antigen and
            alpha fetoprotein and their associations with cancer risk
  JOURNAL   Gut 63 (1), 143-151 (2014)
   PUBMED   23300138
REFERENCE   5  (bases 1 to 5831)
  AUTHORS   Lin,H., Fridy,P.C., Ribeiro,A.A., Choi,J.H., Barma,D.K., Vogel,G.,
            Falck,J.R., Shears,S.B., York,J.D. and Mayr,G.W.
  TITLE     Structural analysis and detection of biological inositol
            pyrophosphates reveal that the family of VIP/diphosphoinositol
            pentakisphosphate kinases are 1/3-kinases
  JOURNAL   J Biol Chem 284 (3), 1863-1872 (2009)
   PUBMED   18981179
REFERENCE   6  (bases 1 to 5831)
  AUTHORS   Choi,J.H., Williams,J., Cho,J., Falck,J.R. and Shears,S.B.
  TITLE     Purification, sequencing, and molecular identification of a
            mammalian PP-InsP5 kinase that is activated when cells are exposed
            to hyperosmotic stress
  JOURNAL   J Biol Chem 282 (42), 30763-30775 (2007)
   PUBMED   17702752
REFERENCE   7  (bases 1 to 5831)
  AUTHORS   Fridy,P.C., Otto,J.C., Dollins,D.E. and York,J.D.
  TITLE     Cloning and characterization of two human VIP1-like inositol
            hexakisphosphate and diphosphoinositol pentakisphosphate kinases
  JOURNAL   J Biol Chem 282 (42), 30754-30762 (2007)
   PUBMED   17690096
REFERENCE   8  (bases 1 to 5831)
  AUTHORS   Washietl,S., Pedersen,J.S., Korbel,J.O., Stocsits,C., Gruber,A.R.,
            Hackermuller,J., Hertel,J., Lindemeyer,M., Reiche,K., Tanzer,A.,
            Ucla,C., Wyss,C., Antonarakis,S.E., Denoeud,F., Lagarde,J.,
            Drenkow,J., Kapranov,P., Gingeras,T.R., Guigo,R., Snyder,M.,
            Gerstein,M.B., Reymond,A., Hofacker,I.L. and Stadler,P.F.
  TITLE     Structured RNAs in the ENCODE selected regions of the human genome
  JOURNAL   Genome Res 17 (6), 852-864 (2007)
   PUBMED   17568003
REFERENCE   9  (bases 1 to 5831)
  AUTHORS   Mulugu,S., Bai,W., Fridy,P.C., Bastidas,R.J., Otto,J.C.,
            Dollins,D.E., Haystead,T.A., Ribeiro,A.A. and York,J.D.
  TITLE     A conserved family of enzymes that phosphorylate inositol
            hexakisphosphate
  JOURNAL   Science 316 (5821), 106-109 (2007)
   PUBMED   17412958
REFERENCE   10 (bases 1 to 5831)
  AUTHORS   Ohler,U., Shomron,N. and Burge,C.B.
  TITLE     Recognition of unknown conserved alternatively spliced exons
  JOURNAL   PLoS Comput Biol 1 (2), 113-122 (2005)
   PUBMED   16110330
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011330.8 and AC019011.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: This gene encodes a dual functional inositol kinase. The
            encoded enzyme converts inositol hexakisphosphate to
            diphosphoinositol pentakisphosphate and diphosphoinositol
            pentakisphosphate to bis-diphosphoinositol tetrakisphosphate. This
            protein may be important for intracellular signaling pathways.
            Alternate splicing results in multiple transcript variants. A
            pseudogene of this gene is found on chromosome 15.[provided by
            RefSeq, Jun 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.123426.1,
                                           SRR1660805.8170.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000420765.6/ ENSP00000400887.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                AC011330.8         59211-59247         c
            38-131              AC011330.8         53838-53931         c
            132-306             AC011330.8         53397-53571         c
            307-502             AC011330.8         52443-52638         c
            503-593             AC011330.8         51901-51991         c
            594-679             AC011330.8         51603-51688         c
            680-834             AC011330.8         51134-51288         c
            835-936             AC011330.8         50892-50993         c
            937-1098            AC011330.8         50266-50427         c
            1099-1220           AC011330.8         50041-50162         c
            1221-1322           AC011330.8         48058-48159         c
            1323-1409           AC011330.8         47109-47195         c
            1410-1485           AC011330.8         46915-46990         c
            1486-1595           AC011330.8         46661-46770         c
            1596-1678           AC011330.8         46492-46574         c
            1679-1810           AC011330.8         46147-46278         c
            1811-1932           AC011330.8         45866-45987         c
            1933-2115           AC011330.8         45410-45592         c
            2116-2257           AC011330.8         43261-43402         c
            2258-2481           AC011330.8         42848-43071         c
            2482-2609           AC011330.8         42396-42523         c
            2610-2724           AC011330.8         42073-42187         c
            2725-2832           AC011330.8         41808-41915         c
            2833-2949           AC019011.7         160243-160359       c
            2950-3114           AC019011.7         159931-160095       c
            3115-3205           AC019011.7         156982-157072       c
            3206-3331           AC019011.7         153386-153511       c
            3332-3394           AC019011.7         152622-152684       c
            3395-3577           AC019011.7         148932-149114       c
            3578-3715           AC019011.7         147314-147451       c
            3716-3829           AC019011.7         127989-128102       c
            3830-5831           AC019011.7         121994-123995       c
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.3"
     gene            1..5831
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /note="diphosphoinositol pentakisphosphate kinase 1"
                     /db_xref="GeneID:9677"
                     /db_xref="HGNC:HGNC:29023"
                     /db_xref="MIM:610979"
     exon            1..37
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            38..131
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            132..306
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             160..4632
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /EC_number="2.7.4.24"
                     /note="isoform 17 is encoded by transcript variant 32;
                     inositol pyrophosphate synthase 1; histidine acid
                     phosphatase domain containing 2A; inositol
                     hexakisphosphate and diphosphoinositol-pentakisphosphate
                     kinase 1; IP6 kinase; insP6 and PP-IP5 kinase 1; histidine
                     acid phosphatase domain-containing protein 2A; VIP1
                     homolog"
                     /codon_start=1
                     /product="inositol hexakisphosphate and
                     diphosphoinositol-pentakisphosphate kinase 1 isoform 17"
                     /protein_id="NP_001381324.1"
                     /db_xref="CCDS:CCDS91989.1"
                     /db_xref="GeneID:9677"
                     /db_xref="HGNC:HGNC:29023"
                     /db_xref="MIM:610979"
                     /translation="MWSLTASEGESTTAHFFLGAGDEGLGTRGIGMRPEESDSELLED
                     EEDEVPPEPQIIVGICAMTKKSKSKPMTQILERLCRFDYLTVVILGEDVILNEPVENW
                     PSCHCLISFHSKGFPLDKAVAYSKLRNPFLINDLAMQYYIQDRREVYRILQEEGIDLP
                     RYAVLNRDPARPEECNLIEGEDQVEVNGAVFPKPFVEKPVSAEDHNVYIYYPSSAGGG
                     SQRLFRKIGSRSSVYSPESSVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSP
                     ALDGKVERDSEGKEIRYPVMLTAMEKLVARKVCVAFKQTVCGFDLLRANGHSFVCDVN
                     GFSFVKNSMKYYDDCAKILGNTIMRELAPQFQIPWSIPTEAEDIPIVPTTSGTMMELR
                     CVIAIIRHGDRTPKQKMKMEVKHPRFFALFEKHGGYKTGKLKLKRPEQLQEVLDITRL
                     LLAELEKEPGGEIEEKTGKLEQLKSVLEMYGHFSGINRKVQLTYYPHGVKASNEGQDP
                     QRETLAPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQGDYAGFPGCGLLRLHS
                     TFRHDLKIYASDEGRVQMTAAAFAKGLLALEGELTPILVQMVKSANMNGLLDSDGDSL
                     SSCQHRVKARLHHILQQDAPFGPEDYDQLAPTRSTSLLNSMTIIQNPVKVCDQVFALI
                     ENLTHQIRERMQDPRSVDLQLYHSETLELMLQRWSKLERDFRQKSGRYDISKIPDIYD
                     CVKYDVQHNGSLGLQGTAELLRLSKALADVVIPQEYGISREEKLEIAVGFCLPLLRKI
                     LLDLQRTHEDESVNKLHPLYSRGVLSPGRHVRTRLYFTSESHVHSLLSVFRYGGLLDE
                     TQDAQWQRALDYLSAISELNYMTQIVIMLYEDNTQDPLSEERFHVELHFSPGVKGVEE
                     EGSAPAGCGFRPASSENEEMKTNQGSMENLCPGKASDEPDRALQTSPQPPEGPGLPRR
                     SPLIRNRKAGSMEVLSETSSSRPGGYRLFSSSRPPTEMKQSGLGSQCTGLFSTTVLGG
                     SSSAPNLQDYARSHGKKLPPASLKHRDELLFVPAVKRFSVSFAKHPTNELLDDQHPVV
                     RLLRSFSSDCTGGRPVSLDATLAHHLHQCSYHLRLFRNWLRSGQDDPECLYGFEGCSM
                     VPTIYPLETLHNALSLRQVSEFLSRVCQRHTDAQAQASAALFDSMHSSQASDNPFSPP
                     RTLHSPPLQLQQRSEKPPWYSSGPSSTVSSAGPSSPTTVDGNSQFGFSDQPSLNSHVA
                     EEHQGLGLLQETPGSGAQELSIEGEQELFEPNQSPQVPPMETSQPYEEVSQPCQEVPD
                     ISQPCQDISEALSQPCQKVPDISQQCQENHDNGNHTCQEVPHISQPCQKSSQLCQKVS
                     EEVCQLCLENSEEVSQPCQGVSVEVGKLVHKFHVGVGSLVQETLVEVGSPAEEIPEEV
                     IQPYQEFSVEVGRLAQETSAINLLSQGIPEIDKPSQEFPEEIDLQAQEVPEEIN"
     exon            307..502
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            503..593
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            594..679
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            680..834
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            835..936
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1098
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1220
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1322
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1409
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1485
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1595
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1596..1678
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1679..1810
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1811..1932
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..2115
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2116..2257
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2258..2481
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2482..2609
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2610..2724
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2725..2832
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2833..2949
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2950..3114
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3115..3205
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3206..3331
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3332..3394
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3395..3577
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3578..3715
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3716..3829
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3830..5831
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5816..5821
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /note="hexamer: TATAAA"
     polyA_site      5831
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /note="major polyA site"
ORIGIN      
        1 tgcctgcagg ttcggctgcg cgagcggagg gaggcgggac tcaagagtag ccttcctcga
       61 ggacctgcct ttcccatttg ctgcctgaag ttaatgtttc ttgctggcca aatcagggac
      121 atgccggcat tagcgggatg agtgggtgtt ccggcaggga tgtggtcatt gacggccagt
      181 gagggcgaga gtaccacggc ccacttcttc cttggagctg gagatgaggg gctgggcacc
      241 cgtggaatag gcatgaggcc agaagagagt gacagcgagc tccttgagga tgaggaggat
      301 gaagtgcctc ctgaacctca gatcattgtt ggcatctgtg ccatgaccaa gaaatccaag
      361 tccaagccaa tgactcaaat cctagagcga ctctgcagat ttgactacct gactgttgtc
      421 attctgggag aagatgtaat ccttaatgaa cctgtggaaa actggccatc ctgccactgc
      481 ctcatctctt tccactccaa aggctttcct ctggacaaag ctgttgctta ctccaagctt
      541 cgaaacccct ttcttatcaa tgatctggcc atgcagtatt acatccaaga taggagggag
      601 gtgtaccgga tcctgcagga agagggtatt gatctgcctc gatatgctgt gctcaaccgt
      661 gatcctgccc ggcctgagga atgcaacctg atagaaggtg aagaccaagt agaggtcaat
      721 ggagctgtct ttcccaagcc ctttgtggag aagccagtga gtgcagaaga ccacaatgtt
      781 tacatctact accccagctc agctggagga ggaagccagc gtctctttcg taagattggc
      841 agccgaagca gtgtttactc tcctgagagc agcgtccgaa agacggggtc gtacatctat
      901 gaggagttta tgccaacaga tggcacagat gtcaaggtgt atacagtggg gccagattat
      961 gcccatgctg aagctagaaa atctccagct ttggatggga aggttgaacg agacagtgag
     1021 gggaaagaga ttcgatatcc agtcatgctg actgccatgg aaaagctggt ggccaggaaa
     1081 gtctgcgtag ctttcaagca aacagtttgt ggatttgacc ttcttcgtgc caatggtcat
     1141 tcctttgtgt gtgatgtcaa tggctttagt tttgtcaaga actcgatgaa atactacgat
     1201 gactgtgcca agattctggg gaacaccata atgcgggagc ttgccccaca gttccagatt
     1261 ccatggtcca tccccacgga ggctgaggac attcccattg ttcccaccac atctggcact
     1321 atgatggaac ttcgttgtgt cattgcaatt attcgtcatg gggatcgtac tcccaagcag
     1381 aagatgaaga tggaagtgaa acacccaagg ttttttgctc tgtttgaaaa acatggtggc
     1441 tacaagacag ggaaattaaa actcaagcga cctgagcagc tccaggaggt gctggatatc
     1501 acaaggctgt tgttggctga actggagaaa gaaccaggtg gtgagatcga ggagaagact
     1561 ggaaaactag agcagctgaa gtctgtactg gagatgtatg gtcacttctc aggtataaac
     1621 cggaaggtac aattgactta ctaccctcat ggagtaaaag cttctaatga ggggcaagat
     1681 ccacagaggg aaactctggc cccatctctg ttgctggtac tgaagtgggg tggagaactg
     1741 actcctgctg gccgtgttca ggctgaggag ctggggcgag cttttcgctg catgtaccct
     1801 ggaggacagg gtgactatgc tggcttccct ggttgtgggc tgcttcgtct ccatagcact
     1861 ttccgccacg atctcaagat ctatgcctct gatgagggtc gtgttcagat gactgctgct
     1921 gccttcgcca agggccttct ggctctagaa ggggagctga cacccatttt ggtgcaaatg
     1981 gtgaagagtg ccaacatgaa tgggctactg gacagcgatg gggattcctt gagcagctgc
     2041 cagcaccggg tgaaggctcg gctgcaccat attctacagc aggatgcgcc ctttggccct
     2101 gaggactacg atcagctggc tcccaccaga agtacttccc tgctcaactc catgactatc
     2161 atccagaatc ctgtgaaggt ctgtgatcag gtatttgccc tgatcgaaaa cctcacccac
     2221 cagatccggg aacgaatgca ggaccccagg tctgtagacc tgcagctcta ccacagtgag
     2281 acactagagc taatgctaca gcgttggagc aagctggagc gtgactttcg acagaagagt
     2341 gggcgctatg atatcagtaa gatccctgac atctatgact gtgtcaagta tgatgtgcag
     2401 cacaatggga gtctgggact tcaaggcaca gcagagttgc tccgtctctc taaggcactg
     2461 gctgatgtgg tcattcccca ggagtacggg atcagtcggg aggagaaact ggaaattgct
     2521 gtgggcttct gtcttccact gttgcggaag atactacttg acctgcagag aacccacgag
     2581 gatgagtctg tcaacaagct gcatcccctg tactcccgag gcgtgctctc cccaggtcgc
     2641 cacgttcgaa cgcgtctcta tttcaccagt gagagccatg tccactccct gctcagtgtc
     2701 ttccgttatg gaggacttct tgatgagacc caggatgcac aatggcagcg agctttggat
     2761 tatcttagtg ccatctcaga gcttaactac atgacccaga ttgtcatcat gctttatgag
     2821 gacaacacac aggatccctt atcagaggaa cggttccatg tggagctaca cttcagcccc
     2881 ggagtgaaag gtgttgagga agaaggcagt gccccggctg gctgtggatt ccgtccagcc
     2941 tcttctgaga atgaggagat gaaaaccaac caaggcagta tggagaacct gtgtccagga
     3001 aaggcatcag atgaaccaga ccgggcattg cagacttcac cccagcctcc tgagggccct
     3061 ggccttccga ggagatcacc cctcattcgt aaccgaaaag ctggttccat ggaggtactt
     3121 tctgagactt catcctcgag gcctggtggc taccggctct tttcatcttc acggccaccc
     3181 acagaaatga agcagagtgg cctaggctca cagtgcacag ggctgttcag caccacagtg
     3241 ctgggtggct cctccagtgc cccgaatctt caggactacg cccgcagcca tggcaaaaag
     3301 ctaccacctg ccagtctgaa gcaccgagat gagctcttgt ttgtcccggc cgtaaaacga
     3361 ttttctgtgt cgtttgcaaa gcatccgact aacgagctgc tggatgacca gcaccctgtg
     3421 gtccggttgc tgcgcagttt ttcctctgac tgtacagggg gccggccagt ctccttggat
     3481 gccacgctgg cgcatcacct gcaccagtgc tcctaccacc tgcgcctctt ccggaactgg
     3541 ctgcgctcag gccaggatga ccccgagtgc ctctacgggt ttgaagggtg ttccatggtg
     3601 cctaccatct accctctgga aacactgcat aatgcccttt ccctacgtca agtgagtgaa
     3661 ttcttgagta gagtctgcca gcgccacact gatgcccagg cacaggcatc tgcagccctc
     3721 tttgattcca tgcacagcag ccaggcctca gataacccat tttctccacc acgtactctt
     3781 cattcacctc ccctgcaact ccagcagcgc tctgagaagc ccccttggta cagcagtggc
     3841 ccttctagca ctgtgtccag tgctggtcct tcttccccta ctacagtaga tggtaactcc
     3901 caatttggct tcagtgatca accctcccta aattcacacg tggctgaaga acatcaaggc
     3961 cttgggctgc tccaggagac ccctgggagt ggagcacaag agctctccat agaaggggag
     4021 caagagcttt ttgaaccaaa tcagtcccca caggtgccac ctatggaaac cagccagcca
     4081 tacgaggagg tcagccagcc atgtcaggag gtccctgaca tcagccagcc atgccaggac
     4141 atttctgagg cgctcagcca gccatgtcag aaggtccctg acatcagcca gcaatgccag
     4201 gagaaccatg acaatggtaa ccacacatgc caggaggtcc ctcacatcag ccagccatgc
     4261 cagaagtcca gccaactgtg ccagaaagtc tctgaggaag tttgccagct atgtctggag
     4321 aactccgagg aggtcagcca gccatgccag ggggtctctg tggaggttgg caagctggtc
     4381 cataagttcc atgtaggggt tggtagcttg gtccaggaaa cccttgtaga agttggcagc
     4441 ccagctgaag agatccctga ggaggtcatc cagccatacc aggagttctc tgtggaggtt
     4501 ggcaggctgg cccaggagac ttctgcgatc aatctgttat ctcagggcat ccctgagatt
     4561 gataaaccat cccaagagtt ccctgaggag attgatctgc aggcccagga ggtccctgag
     4621 gagataaatt agaagtcctg ggtggtccct gaagtgattg atcagctgcc tggagagggt
     4681 attcctcaag cccagcatcc atctggtgat ccaaaccctc agagccagtc tctagcccat
     4741 gaccagcact caccccttcc accagcaaca tgtgattaat tttctcatta gtggtatcac
     4801 actataccag ccatttgagc cagcaacctt ttctgttggc taactcactg gccagctctc
     4861 accagcggtg tctggggaag tagttctctt tgtatgaagc atacctgtgc cagagctgtg
     4921 gttgaggagg agccagtttt aggttcgaag aagccattgg ctcctcactt agccattaga
     4981 cttgaatagg atttccttgg ggtgggttgg tctgtcatta cccagccttc tctgagcatc
     5041 ctaggaaatc acagattgtt aaaggaaatg ccgcttcact gctgaagaca ccatctggcg
     5101 acagcaaatg caaaagaggg gactctaggg tcttcacttt tctggggaaa tgttcacgac
     5161 ttctcaaggt acgcttagac catatgtgca ttcaggggac tcttgtcttt gccagcactc
     5221 ctcaggtgag cccgggatgg ctatctgaaa tgtctggaaa atagagtccc ttccccaaac
     5281 atctgacatg gccactaaat ctttagactt gccttataga tccttccata tcatccccaa
     5341 gtgccccttc tgcctcagtc ctgttcctct gggcaatagc tctagggaaa agtaggtatt
     5401 agactgctgt gcaaaattca gagcaactac ttaaaatgct ctagaggatt cttagccagt
     5461 ctgtgaaagt gggcctcctc tgtggtgggg ctgtttggct taggagatgc tgtagtagta
     5521 gagataatca ggttctattt taagcaatca gcagagatca atattgtaca gatacaagca
     5581 aaggtttaat aaatatatat atatatattt atttctgtag ttgtgccagg gacagactgg
     5641 ccaaagacca aacactgcag ggtccccaag aaatttgtcc ttatatcatt gtgtctgagg
     5701 gccagatatg attatgaagc tttttccaaa gatccaggga tgggaatggg agtgggtagg
     5761 aggggtaatg cggtcattgg agtcgggggc tggaacatta tgagtgctca ataaatataa
     5821 actaatgaga a
//
