LOCUS       NM_001354393            5606 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens diphosphoinositol pentakisphosphate kinase 1
            (PPIP5K1), transcript variant 19, mRNA.
ACCESSION   NM_001354393 XM_017022752
VERSION     NM_001354393.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Gu,C., Nguyen,H.N., Hofer,A., Jessen,H.J., Dai,X., Wang,H. and
            Shears,S.B.
  TITLE     The Significance of the Bifunctional Kinase/Phosphatase Activities
            of Diphosphoinositol Pentakisphosphate Kinases (PPIP5Ks) for
            Coupling Inositol Pyrophosphate Cell Signaling to Cellular
            Phosphate Homeostasis
  JOURNAL   J Biol Chem 292 (11), 4544-4555 (2017)
   PUBMED   28126903
  REMARK    GeneRIF: This study characterized kinetic properties of the
            bifunctional inositol pyrophosphate 5-diphosphoinositol
            1,2,3,4,6-pentakisphosphatekinase/inositol pyrophosphate,
            1,5-bisdiphosphoinositol 2,3,4,6-tetrakisphosphate phosphatase
            activities of full-length diphosphoinositol pentakisphosphate
            kinase 1 and 2.
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Shears,S.B., Baughman,B.M., Gu,C., Nair,V.S. and Wang,H.
  TITLE     The significance of the 1-kinase/1-phosphatase activities of the
            PPIP5K family
  JOURNAL   Adv Biol Regul 63, 98-106 (2017)
   PUBMED   27776974
  REMARK    GeneRIF: PPIP5K is a rare example of a single protein that
            catalyzes a kinase/phosphatase futile cycle. (Review)
            Review article
REFERENCE   3  (bases 1 to 5606)
  AUTHORS   Machkalyan,G., Trieu,P., Petrin,D., Hebert,T.E. and Miller,G.J.
  TITLE     PPIP5K1 interacts with the exocyst complex through a C-terminal
            intrinsically disordered domain and regulates cell motility
  JOURNAL   Cell Signal 28 (5), 401-411 (2016)
   PUBMED   26854614
  REMARK    GeneRIF: results suggest that PPIP5K1 might play an important role
            in regulating function of exocyst complex in establishing cellular
            polarity and directional migration of cells
REFERENCE   4  (bases 1 to 5606)
  AUTHORS   He,M., Wu,C., Xu,J., Guo,H., Yang,H., Zhang,X., Sun,J., Yu,D.,
            Zhou,L., Peng,T., He,Y., Gao,Y., Yuan,J., Deng,Q., Dai,X., Tan,A.,
            Feng,Y., Zhang,H., Min,X., Yang,X., Zhu,J., Zhai,K., Chang,J.,
            Qin,X., Tan,W., Hu,Y., Lang,M., Tao,S., Li,Y., Li,Y., Feng,J.,
            Li,D., Kim,S.T., Zhang,S., Zhang,H., Zheng,S.L., Gui,L., Wang,Y.,
            Wei,S., Wang,F., Fang,W., Liang,Y., Zhai,Y., Chen,W., Miao,X.,
            Zhou,G., Hu,F.B., Lin,D., Mo,Z. and Wu,T.
  TITLE     A genome wide association study of genetic loci that influence
            tumour biomarkers cancer antigen 19-9, carcinoembryonic antigen and
            alpha fetoprotein and their associations with cancer risk
  JOURNAL   Gut 63 (1), 143-151 (2014)
   PUBMED   23300138
REFERENCE   5  (bases 1 to 5606)
  AUTHORS   Lin,H., Fridy,P.C., Ribeiro,A.A., Choi,J.H., Barma,D.K., Vogel,G.,
            Falck,J.R., Shears,S.B., York,J.D. and Mayr,G.W.
  TITLE     Structural analysis and detection of biological inositol
            pyrophosphates reveal that the family of VIP/diphosphoinositol
            pentakisphosphate kinases are 1/3-kinases
  JOURNAL   J Biol Chem 284 (3), 1863-1872 (2009)
   PUBMED   18981179
REFERENCE   6  (bases 1 to 5606)
  AUTHORS   Choi,J.H., Williams,J., Cho,J., Falck,J.R. and Shears,S.B.
  TITLE     Purification, sequencing, and molecular identification of a
            mammalian PP-InsP5 kinase that is activated when cells are exposed
            to hyperosmotic stress
  JOURNAL   J Biol Chem 282 (42), 30763-30775 (2007)
   PUBMED   17702752
REFERENCE   7  (bases 1 to 5606)
  AUTHORS   Fridy,P.C., Otto,J.C., Dollins,D.E. and York,J.D.
  TITLE     Cloning and characterization of two human VIP1-like inositol
            hexakisphosphate and diphosphoinositol pentakisphosphate kinases
  JOURNAL   J Biol Chem 282 (42), 30754-30762 (2007)
   PUBMED   17690096
REFERENCE   8  (bases 1 to 5606)
  AUTHORS   Washietl,S., Pedersen,J.S., Korbel,J.O., Stocsits,C., Gruber,A.R.,
            Hackermuller,J., Hertel,J., Lindemeyer,M., Reiche,K., Tanzer,A.,
            Ucla,C., Wyss,C., Antonarakis,S.E., Denoeud,F., Lagarde,J.,
            Drenkow,J., Kapranov,P., Gingeras,T.R., Guigo,R., Snyder,M.,
            Gerstein,M.B., Reymond,A., Hofacker,I.L. and Stadler,P.F.
  TITLE     Structured RNAs in the ENCODE selected regions of the human genome
  JOURNAL   Genome Res 17 (6), 852-864 (2007)
   PUBMED   17568003
REFERENCE   9  (bases 1 to 5606)
  AUTHORS   Mulugu,S., Bai,W., Fridy,P.C., Bastidas,R.J., Otto,J.C.,
            Dollins,D.E., Haystead,T.A., Ribeiro,A.A. and York,J.D.
  TITLE     A conserved family of enzymes that phosphorylate inositol
            hexakisphosphate
  JOURNAL   Science 316 (5821), 106-109 (2007)
   PUBMED   17412958
REFERENCE   10 (bases 1 to 5606)
  AUTHORS   Ohler,U., Shomron,N. and Burge,C.B.
  TITLE     Recognition of unknown conserved alternatively spliced exons
  JOURNAL   PLoS Comput Biol 1 (2), 113-122 (2005)
   PUBMED   16110330
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011330.8 and AC019011.7.
            
            On Aug 13, 2020 this sequence version replaced NM_001354393.1.
            
            Summary: This gene encodes a dual functional inositol kinase. The
            encoded enzyme converts inositol hexakisphosphate to
            diphosphoinositol pentakisphosphate and diphosphoinositol
            pentakisphosphate to bis-diphosphoinositol tetrakisphosphate. This
            protein may be important for intracellular signaling pathways.
            Alternate splicing results in multiple transcript variants. A
            pseudogene of this gene is found on chromosome 15.[provided by
            RefSeq, Jun 2010].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.3223698.1,
                                           SRR14038195.1165003.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-82                AC011330.8         59211-59292         c
            83-176              AC011330.8         53838-53931         c
            177-351             AC011330.8         53397-53571         c
            352-547             AC011330.8         52443-52638         c
            548-638             AC011330.8         51901-51991         c
            639-724             AC011330.8         51603-51688         c
            725-879             AC011330.8         51134-51288         c
            880-981             AC011330.8         50892-50993         c
            982-1143            AC011330.8         50266-50427         c
            1144-1265           AC011330.8         50041-50162         c
            1266-1367           AC011330.8         48058-48159         c
            1368-1454           AC011330.8         47109-47195         c
            1455-1530           AC011330.8         46915-46990         c
            1531-1640           AC011330.8         46661-46770         c
            1641-1723           AC011330.8         46492-46574         c
            1724-1855           AC011330.8         46147-46278         c
            1856-1977           AC011330.8         45866-45987         c
            1978-2160           AC011330.8         45410-45592         c
            2161-2302           AC011330.8         43261-43402         c
            2303-2526           AC011330.8         42848-43071         c
            2527-2630           AC011330.8         42420-42523         c
            2631-2745           AC011330.8         42073-42187         c
            2746-2853           AC011330.8         41808-41915         c
            2854-2970           AC019011.7         160243-160359       c
            2971-3135           AC019011.7         159931-160095       c
            3136-3226           AC019011.7         156982-157072       c
            3227-3352           AC019011.7         153386-153511       c
            3353-3490           AC019011.7         147314-147451       c
            3491-3604           AC019011.7         127989-128102       c
            3605-5606           AC019011.7         121994-123995       c
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.3"
     gene            1..5606
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /note="diphosphoinositol pentakisphosphate kinase 1"
                     /db_xref="GeneID:9677"
                     /db_xref="HGNC:HGNC:29023"
                     /db_xref="MIM:610979"
     exon            1..82
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            83..176
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            177..351
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             205..4407
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /EC_number="2.7.4.24"
                     /note="isoform 9 is encoded by transcript variant 19;
                     inositol pyrophosphate synthase 1; histidine acid
                     phosphatase domain containing 2A; inositol
                     hexakisphosphate and diphosphoinositol-pentakisphosphate
                     kinase 1; IP6 kinase; insP6 and PP-IP5 kinase 1; histidine
                     acid phosphatase domain-containing protein 2A; VIP1
                     homolog"
                     /codon_start=1
                     /product="inositol hexakisphosphate and
                     diphosphoinositol-pentakisphosphate kinase 1 isoform 9"
                     /protein_id="NP_001341322.1"
                     /db_xref="GeneID:9677"
                     /db_xref="HGNC:HGNC:29023"
                     /db_xref="MIM:610979"
                     /translation="MWSLTASEGESTTAHFFLGAGDEGLGTRGIGMRPEESDSELLED
                     EEDEVPPEPQIIVGICAMTKKSKSKPMTQILERLCRFDYLTVVILGEDVILNEPVENW
                     PSCHCLISFHSKGFPLDKAVAYSKLRNPFLINDLAMQYYIQDRREVYRILQEEGIDLP
                     RYAVLNRDPARPEECNLIEGEDQVEVNGAVFPKPFVEKPVSAEDHNVYIYYPSSAGGG
                     SQRLFRKIGSRSSVYSPESSVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSP
                     ALDGKVERDSEGKEIRYPVMLTAMEKLVARKVCVAFKQTVCGFDLLRANGHSFVCDVN
                     GFSFVKNSMKYYDDCAKILGNTIMRELAPQFQIPWSIPTEAEDIPIVPTTSGTMMELR
                     CVIAIIRHGDRTPKQKMKMEVKHPRFFALFEKHGGYKTGKLKLKRPEQLQEVLDITRL
                     LLAELEKEPGGEIEEKTGKLEQLKSVLEMYGHFSGINRKVQLTYYPHGVKASNEGQDP
                     QRETLAPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQGDYAGFPGCGLLRLHS
                     TFRHDLKIYASDEGRVQMTAAAFAKGLLALEGELTPILVQMVKSANMNGLLDSDGDSL
                     SSCQHRVKARLHHILQQDAPFGPEDYDQLAPTRSTSLLNSMTIIQNPVKVCDQVFALI
                     ENLTHQIRERMQDPRSVDLQLYHSETLELMLQRWSKLERDFRQKSGRYDISKIPDIYD
                     CVKYDVQHNGSLGLQGTAELLRLSKALADVVIPQEYGISREEKLEIAVGFCLPLLRKI
                     LLDLQRTHEDEYSRGVLSPGRHVRTRLYFTSESHVHSLLSVFRYGGLLDETQDAQWQR
                     ALDYLSAISELNYMTQIVIMLYEDNTQDPLSEERFHVELHFSPGVKGVEEEGSAPAGC
                     GFRPASSENEEMKTNQGSMENLCPGKASDEPDRALQTSPQPPEGPGLPRRSPLIRNRK
                     AGSMEVLSETSSSRPGGYRLFSSSRPPTEMKQSGLGSQCTGLFSTTVLGGSSSAPNLQ
                     DYARSHGKKLPPASLKHRDGFEGCSMVPTIYPLETLHNALSLRQVSEFLSRVCQRHTD
                     AQAQASAALFDSMHSSQASDNPFSPPRTLHSPPLQLQQRSEKPPWYSSGPSSTVSSAG
                     PSSPTTVDGNSQFGFSDQPSLNSHVAEEHQGLGLLQETPGSGAQELSIEGEQELFEPN
                     QSPQVPPMETSQPYEEVSQPCQEVPDISQPCQDISEALSQPCQKVPDISQQCQENHDN
                     GNHTCQEVPHISQPCQKSSQLCQKVSEEVCQLCLENSEEVSQPCQGVSVEVGKLVHKF
                     HVGVGSLVQETLVEVGSPAEEIPEEVIQPYQEFSVEVGRLAQETSAINLLSQGIPEID
                     KPSQEFPEEIDLQAQEVPEEIN"
     exon            352..547
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            548..638
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            639..724
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            725..879
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            880..981
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1143
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1265
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1266..1367
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1454
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1530
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1640
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1723
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1724..1855
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1856..1977
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1978..2160
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2161..2302
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2303..2526
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2527..2630
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2631..2745
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2746..2853
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2854..2970
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2971..3135
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3136..3226
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3227..3352
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3353..3490
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3491..3604
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3605..5606
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5591..5596
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /note="hexamer: TATAAA"
     polyA_site      5606
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /note="major polyA site"
ORIGIN      
        1 accgcaaaga agtcgcgctt tgattgggcc gctgtcaggt gtcgctgcct gcaggttcgg
       61 ctgcgcgagc ggagggaggc gggactcaag agtagccttc ctcgaggacc tgcctttccc
      121 atttgctgcc tgaagttaat gtttcttgct ggccaaatca gggacatgcc ggcattagcg
      181 ggatgagtgg gtgttccggc agggatgtgg tcattgacgg ccagtgaggg cgagagtacc
      241 acggcccact tcttccttgg agctggagat gaggggctgg gcacccgtgg aataggcatg
      301 aggccagaag agagtgacag cgagctcctt gaggatgagg aggatgaagt gcctcctgaa
      361 cctcagatca ttgttggcat ctgtgccatg accaagaaat ccaagtccaa gccaatgact
      421 caaatcctag agcgactctg cagatttgac tacctgactg ttgtcattct gggagaagat
      481 gtaatcctta atgaacctgt ggaaaactgg ccatcctgcc actgcctcat ctctttccac
      541 tccaaaggct ttcctctgga caaagctgtt gcttactcca agcttcgaaa cccctttctt
      601 atcaatgatc tggccatgca gtattacatc caagatagga gggaggtgta ccggatcctg
      661 caggaagagg gtattgatct gcctcgatat gctgtgctca accgtgatcc tgcccggcct
      721 gaggaatgca acctgataga aggtgaagac caagtagagg tcaatggagc tgtctttccc
      781 aagccctttg tggagaagcc agtgagtgca gaagaccaca atgtttacat ctactacccc
      841 agctcagctg gaggaggaag ccagcgtctc tttcgtaaga ttggcagccg aagcagtgtt
      901 tactctcctg agagcagcgt ccgaaagacg gggtcgtaca tctatgagga gtttatgcca
      961 acagatggca cagatgtcaa ggtgtataca gtggggccag attatgccca tgctgaagct
     1021 agaaaatctc cagctttgga tgggaaggtt gaacgagaca gtgaggggaa agagattcga
     1081 tatccagtca tgctgactgc catggaaaag ctggtggcca ggaaagtctg cgtagctttc
     1141 aagcaaacag tttgtggatt tgaccttctt cgtgccaatg gtcattcctt tgtgtgtgat
     1201 gtcaatggct ttagttttgt caagaactcg atgaaatact acgatgactg tgccaagatt
     1261 ctggggaaca ccataatgcg ggagcttgcc ccacagttcc agattccatg gtccatcccc
     1321 acggaggctg aggacattcc cattgttccc accacatctg gcactatgat ggaacttcgt
     1381 tgtgtcattg caattattcg tcatggggat cgtactccca agcagaagat gaagatggaa
     1441 gtgaaacacc caaggttttt tgctctgttt gaaaaacatg gtggctacaa gacagggaaa
     1501 ttaaaactca agcgacctga gcagctccag gaggtgctgg atatcacaag gctgttgttg
     1561 gctgaactgg agaaagaacc aggtggtgag atcgaggaga agactggaaa actagagcag
     1621 ctgaagtctg tactggagat gtatggtcac ttctcaggta taaaccggaa ggtacaattg
     1681 acttactacc ctcatggagt aaaagcttct aatgaggggc aagatccaca gagggaaact
     1741 ctggccccat ctctgttgct ggtactgaag tggggtggag aactgactcc tgctggccgt
     1801 gttcaggctg aggagctggg gcgagctttt cgctgcatgt accctggagg acagggtgac
     1861 tatgctggct tccctggttg tgggctgctt cgtctccata gcactttccg ccacgatctc
     1921 aagatctatg cctctgatga gggtcgtgtt cagatgactg ctgctgcctt cgccaagggc
     1981 cttctggctc tagaagggga gctgacaccc attttggtgc aaatggtgaa gagtgccaac
     2041 atgaatgggc tactggacag cgatggggat tccttgagca gctgccagca ccgggtgaag
     2101 gctcggctgc accatattct acagcaggat gcgccctttg gccctgagga ctacgatcag
     2161 ctggctccca ccagaagtac ttccctgctc aactccatga ctatcatcca gaatcctgtg
     2221 aaggtctgtg atcaggtatt tgccctgatc gaaaacctca cccaccagat ccgggaacga
     2281 atgcaggacc ccaggtctgt agacctgcag ctctaccaca gtgagacact agagctaatg
     2341 ctacagcgtt ggagcaagct ggagcgtgac tttcgacaga agagtgggcg ctatgatatc
     2401 agtaagatcc ctgacatcta tgactgtgtc aagtatgatg tgcagcacaa tgggagtctg
     2461 ggacttcaag gcacagcaga gttgctccgt ctctctaagg cactggctga tgtggtcatt
     2521 ccccaggagt acgggatcag tcgggaggag aaactggaaa ttgctgtggg cttctgtctt
     2581 ccactgttgc ggaagatact acttgacctg cagagaaccc acgaggatga gtactcccga
     2641 ggcgtgctct ccccaggtcg ccacgttcga acgcgtctct atttcaccag tgagagccat
     2701 gtccactccc tgctcagtgt cttccgttat ggaggacttc ttgatgagac ccaggatgca
     2761 caatggcagc gagctttgga ttatcttagt gccatctcag agcttaacta catgacccag
     2821 attgtcatca tgctttatga ggacaacaca caggatccct tatcagagga acggttccat
     2881 gtggagctac acttcagccc cggagtgaaa ggtgttgagg aagaaggcag tgccccggct
     2941 ggctgtggat tccgtccagc ctcttctgag aatgaggaga tgaaaaccaa ccaaggcagt
     3001 atggagaacc tgtgtccagg aaaggcatca gatgaaccag accgggcatt gcagacttca
     3061 ccccagcctc ctgagggccc tggccttccg aggagatcac ccctcattcg taaccgaaaa
     3121 gctggttcca tggaggtact ttctgagact tcatcctcga ggcctggtgg ctaccggctc
     3181 ttttcatctt cacggccacc cacagaaatg aagcagagtg gcctaggctc acagtgcaca
     3241 gggctgttca gcaccacagt gctgggtggc tcctccagtg ccccgaatct tcaggactac
     3301 gcccgcagcc atggcaaaaa gctaccacct gccagtctga agcaccgaga tgggtttgaa
     3361 gggtgttcca tggtgcctac catctaccct ctggaaacac tgcataatgc cctttcccta
     3421 cgtcaagtga gtgaattctt gagtagagtc tgccagcgcc acactgatgc ccaggcacag
     3481 gcatctgcag ccctctttga ttccatgcac agcagccagg cctcagataa cccattttct
     3541 ccaccacgta ctcttcattc acctcccctg caactccagc agcgctctga gaagccccct
     3601 tggtacagca gtggcccttc tagcactgtg tccagtgctg gtccttcttc ccctactaca
     3661 gtagatggta actcccaatt tggcttcagt gatcaaccct ccctaaattc acacgtggct
     3721 gaagaacatc aaggccttgg gctgctccag gagacccctg ggagtggagc acaagagctc
     3781 tccatagaag gggagcaaga gctttttgaa ccaaatcagt ccccacaggt gccacctatg
     3841 gaaaccagcc agccatacga ggaggtcagc cagccatgtc aggaggtccc tgacatcagc
     3901 cagccatgcc aggacatttc tgaggcgctc agccagccat gtcagaaggt ccctgacatc
     3961 agccagcaat gccaggagaa ccatgacaat ggtaaccaca catgccagga ggtccctcac
     4021 atcagccagc catgccagaa gtccagccaa ctgtgccaga aagtctctga ggaagtttgc
     4081 cagctatgtc tggagaactc cgaggaggtc agccagccat gccagggggt ctctgtggag
     4141 gttggcaagc tggtccataa gttccatgta ggggttggta gcttggtcca ggaaaccctt
     4201 gtagaagttg gcagcccagc tgaagagatc cctgaggagg tcatccagcc ataccaggag
     4261 ttctctgtgg aggttggcag gctggcccag gagacttctg cgatcaatct gttatctcag
     4321 ggcatccctg agattgataa accatcccaa gagttccctg aggagattga tctgcaggcc
     4381 caggaggtcc ctgaggagat aaattagaag tcctgggtgg tccctgaagt gattgatcag
     4441 ctgcctggag agggtattcc tcaagcccag catccatctg gtgatccaaa ccctcagagc
     4501 cagtctctag cccatgacca gcactcaccc cttccaccag caacatgtga ttaattttct
     4561 cattagtggt atcacactat accagccatt tgagccagca accttttctg ttggctaact
     4621 cactggccag ctctcaccag cggtgtctgg ggaagtagtt ctctttgtat gaagcatacc
     4681 tgtgccagag ctgtggttga ggaggagcca gttttaggtt cgaagaagcc attggctcct
     4741 cacttagcca ttagacttga ataggatttc cttggggtgg gttggtctgt cattacccag
     4801 ccttctctga gcatcctagg aaatcacaga ttgttaaagg aaatgccgct tcactgctga
     4861 agacaccatc tggcgacagc aaatgcaaaa gaggggactc tagggtcttc acttttctgg
     4921 ggaaatgttc acgacttctc aaggtacgct tagaccatat gtgcattcag gggactcttg
     4981 tctttgccag cactcctcag gtgagcccgg gatggctatc tgaaatgtct ggaaaataga
     5041 gtcccttccc caaacatctg acatggccac taaatcttta gacttgcctt atagatcctt
     5101 ccatatcatc cccaagtgcc ccttctgcct cagtcctgtt cctctgggca atagctctag
     5161 ggaaaagtag gtattagact gctgtgcaaa attcagagca actacttaaa atgctctaga
     5221 ggattcttag ccagtctgtg aaagtgggcc tcctctgtgg tggggctgtt tggcttagga
     5281 gatgctgtag tagtagagat aatcaggttc tattttaagc aatcagcaga gatcaatatt
     5341 gtacagatac aagcaaaggt ttaataaata tatatatata tatttatttc tgtagttgtg
     5401 ccagggacag actggccaaa gaccaaacac tgcagggtcc ccaagaaatt tgtccttata
     5461 tcattgtgtc tgagggccag atatgattat gaagcttttt ccaaagatcc agggatggga
     5521 atgggagtgg gtaggagggg taatgcggtc attggagtcg ggggctggaa cattatgagt
     5581 gctcaataaa tataaactaa tgagaa
//
