LOCUS       NM_001354386            5673 bp    mRNA    linear   PRI 08-APR-2024
DEFINITION  Homo sapiens diphosphoinositol pentakisphosphate kinase 1
            (PPIP5K1), transcript variant 12, mRNA.
ACCESSION   NM_001354386 XM_017022740
VERSION     NM_001354386.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Gu,C., Nguyen,H.N., Hofer,A., Jessen,H.J., Dai,X., Wang,H. and
            Shears,S.B.
  TITLE     The Significance of the Bifunctional Kinase/Phosphatase Activities
            of Diphosphoinositol Pentakisphosphate Kinases (PPIP5Ks) for
            Coupling Inositol Pyrophosphate Cell Signaling to Cellular
            Phosphate Homeostasis
  JOURNAL   J Biol Chem 292 (11), 4544-4555 (2017)
   PUBMED   28126903
  REMARK    GeneRIF: This study characterized kinetic properties of the
            bifunctional inositol pyrophosphate 5-diphosphoinositol
            1,2,3,4,6-pentakisphosphatekinase/inositol pyrophosphate,
            1,5-bisdiphosphoinositol 2,3,4,6-tetrakisphosphate phosphatase
            activities of full-length diphosphoinositol pentakisphosphate
            kinase 1 and 2.
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Shears,S.B., Baughman,B.M., Gu,C., Nair,V.S. and Wang,H.
  TITLE     The significance of the 1-kinase/1-phosphatase activities of the
            PPIP5K family
  JOURNAL   Adv Biol Regul 63, 98-106 (2017)
   PUBMED   27776974
  REMARK    GeneRIF: PPIP5K is a rare example of a single protein that
            catalyzes a kinase/phosphatase futile cycle. (Review)
            Review article
REFERENCE   3  (bases 1 to 5673)
  AUTHORS   Machkalyan,G., Trieu,P., Petrin,D., Hebert,T.E. and Miller,G.J.
  TITLE     PPIP5K1 interacts with the exocyst complex through a C-terminal
            intrinsically disordered domain and regulates cell motility
  JOURNAL   Cell Signal 28 (5), 401-411 (2016)
   PUBMED   26854614
  REMARK    GeneRIF: results suggest that PPIP5K1 might play an important role
            in regulating function of exocyst complex in establishing cellular
            polarity and directional migration of cells
REFERENCE   4  (bases 1 to 5673)
  AUTHORS   He,M., Wu,C., Xu,J., Guo,H., Yang,H., Zhang,X., Sun,J., Yu,D.,
            Zhou,L., Peng,T., He,Y., Gao,Y., Yuan,J., Deng,Q., Dai,X., Tan,A.,
            Feng,Y., Zhang,H., Min,X., Yang,X., Zhu,J., Zhai,K., Chang,J.,
            Qin,X., Tan,W., Hu,Y., Lang,M., Tao,S., Li,Y., Li,Y., Feng,J.,
            Li,D., Kim,S.T., Zhang,S., Zhang,H., Zheng,S.L., Gui,L., Wang,Y.,
            Wei,S., Wang,F., Fang,W., Liang,Y., Zhai,Y., Chen,W., Miao,X.,
            Zhou,G., Hu,F.B., Lin,D., Mo,Z. and Wu,T.
  TITLE     A genome wide association study of genetic loci that influence
            tumour biomarkers cancer antigen 19-9, carcinoembryonic antigen and
            alpha fetoprotein and their associations with cancer risk
  JOURNAL   Gut 63 (1), 143-151 (2014)
   PUBMED   23300138
REFERENCE   5  (bases 1 to 5673)
  AUTHORS   Lin,H., Fridy,P.C., Ribeiro,A.A., Choi,J.H., Barma,D.K., Vogel,G.,
            Falck,J.R., Shears,S.B., York,J.D. and Mayr,G.W.
  TITLE     Structural analysis and detection of biological inositol
            pyrophosphates reveal that the family of VIP/diphosphoinositol
            pentakisphosphate kinases are 1/3-kinases
  JOURNAL   J Biol Chem 284 (3), 1863-1872 (2009)
   PUBMED   18981179
REFERENCE   6  (bases 1 to 5673)
  AUTHORS   Choi,J.H., Williams,J., Cho,J., Falck,J.R. and Shears,S.B.
  TITLE     Purification, sequencing, and molecular identification of a
            mammalian PP-InsP5 kinase that is activated when cells are exposed
            to hyperosmotic stress
  JOURNAL   J Biol Chem 282 (42), 30763-30775 (2007)
   PUBMED   17702752
REFERENCE   7  (bases 1 to 5673)
  AUTHORS   Fridy,P.C., Otto,J.C., Dollins,D.E. and York,J.D.
  TITLE     Cloning and characterization of two human VIP1-like inositol
            hexakisphosphate and diphosphoinositol pentakisphosphate kinases
  JOURNAL   J Biol Chem 282 (42), 30754-30762 (2007)
   PUBMED   17690096
REFERENCE   8  (bases 1 to 5673)
  AUTHORS   Washietl,S., Pedersen,J.S., Korbel,J.O., Stocsits,C., Gruber,A.R.,
            Hackermuller,J., Hertel,J., Lindemeyer,M., Reiche,K., Tanzer,A.,
            Ucla,C., Wyss,C., Antonarakis,S.E., Denoeud,F., Lagarde,J.,
            Drenkow,J., Kapranov,P., Gingeras,T.R., Guigo,R., Snyder,M.,
            Gerstein,M.B., Reymond,A., Hofacker,I.L. and Stadler,P.F.
  TITLE     Structured RNAs in the ENCODE selected regions of the human genome
  JOURNAL   Genome Res 17 (6), 852-864 (2007)
   PUBMED   17568003
REFERENCE   9  (bases 1 to 5673)
  AUTHORS   Mulugu,S., Bai,W., Fridy,P.C., Bastidas,R.J., Otto,J.C.,
            Dollins,D.E., Haystead,T.A., Ribeiro,A.A. and York,J.D.
  TITLE     A conserved family of enzymes that phosphorylate inositol
            hexakisphosphate
  JOURNAL   Science 316 (5821), 106-109 (2007)
   PUBMED   17412958
REFERENCE   10 (bases 1 to 5673)
  AUTHORS   Ohler,U., Shomron,N. and Burge,C.B.
  TITLE     Recognition of unknown conserved alternatively spliced exons
  JOURNAL   PLoS Comput Biol 1 (2), 113-122 (2005)
   PUBMED   16110330
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011330.8 and AC019011.7.
            
            On Apr 19, 2021 this sequence version replaced NM_001354386.1.
            
            Summary: This gene encodes a dual functional inositol kinase. The
            encoded enzyme converts inositol hexakisphosphate to
            diphosphoinositol pentakisphosphate and diphosphoinositol
            pentakisphosphate to bis-diphosphoinositol tetrakisphosphate. This
            protein may be important for intracellular signaling pathways.
            Alternate splicing results in multiple transcript variants. A
            pseudogene of this gene is found on chromosome 15.[provided by
            RefSeq, Jun 2010].
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.1663829.1,
                                           SRR1803611.141932.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-62                AC011330.8         58921-58982         c
            63-156              AC011330.8         53838-53931         c
            157-331             AC011330.8         53397-53571         c
            332-527             AC011330.8         52443-52638         c
            528-618             AC011330.8         51901-51991         c
            619-704             AC011330.8         51603-51688         c
            705-859             AC011330.8         51134-51288         c
            860-961             AC011330.8         50892-50993         c
            962-1123            AC011330.8         50266-50427         c
            1124-1245           AC011330.8         50041-50162         c
            1246-1347           AC011330.8         48058-48159         c
            1348-1434           AC011330.8         47109-47195         c
            1435-1510           AC011330.8         46915-46990         c
            1511-1620           AC011330.8         46661-46770         c
            1621-1703           AC011330.8         46492-46574         c
            1704-1835           AC011330.8         46147-46278         c
            1836-1957           AC011330.8         45866-45987         c
            1958-2140           AC011330.8         45410-45592         c
            2141-2282           AC011330.8         43261-43402         c
            2283-2506           AC011330.8         42848-43071         c
            2507-2634           AC011330.8         42396-42523         c
            2635-2749           AC011330.8         42073-42187         c
            2750-2857           AC011330.8         41808-41915         c
            2858-2974           AC019011.7         160243-160359       c
            2975-3139           AC019011.7         159931-160095       c
            3140-3230           AC019011.7         156982-157072       c
            3231-3356           AC019011.7         153386-153511       c
            3357-3419           AC019011.7         152622-152684       c
            3420-3557           AC019011.7         147314-147451       c
            3558-3671           AC019011.7         127989-128102       c
            3672-5673           AC019011.7         121994-123995       c
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.3"
     gene            1..5673
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /note="diphosphoinositol pentakisphosphate kinase 1"
                     /db_xref="GeneID:9677"
                     /db_xref="HGNC:HGNC:29023"
                     /db_xref="MIM:610979"
     exon            1..62
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            63..156
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            157..331
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             185..4474
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /EC_number="2.7.4.24"
                     /note="isoform 7 is encoded by transcript variant 12;
                     inositol pyrophosphate synthase 1; histidine acid
                     phosphatase domain containing 2A; inositol
                     hexakisphosphate and diphosphoinositol-pentakisphosphate
                     kinase 1; IP6 kinase; insP6 and PP-IP5 kinase 1; histidine
                     acid phosphatase domain-containing protein 2A; VIP1
                     homolog"
                     /codon_start=1
                     /product="inositol hexakisphosphate and
                     diphosphoinositol-pentakisphosphate kinase 1 isoform 7"
                     /protein_id="NP_001341315.1"
                     /db_xref="CCDS:CCDS86452.1"
                     /db_xref="GeneID:9677"
                     /db_xref="HGNC:HGNC:29023"
                     /db_xref="MIM:610979"
                     /translation="MWSLTASEGESTTAHFFLGAGDEGLGTRGIGMRPEESDSELLED
                     EEDEVPPEPQIIVGICAMTKKSKSKPMTQILERLCRFDYLTVVILGEDVILNEPVENW
                     PSCHCLISFHSKGFPLDKAVAYSKLRNPFLINDLAMQYYIQDRREVYRILQEEGIDLP
                     RYAVLNRDPARPEECNLIEGEDQVEVNGAVFPKPFVEKPVSAEDHNVYIYYPSSAGGG
                     SQRLFRKIGSRSSVYSPESSVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSP
                     ALDGKVERDSEGKEIRYPVMLTAMEKLVARKVCVAFKQTVCGFDLLRANGHSFVCDVN
                     GFSFVKNSMKYYDDCAKILGNTIMRELAPQFQIPWSIPTEAEDIPIVPTTSGTMMELR
                     CVIAIIRHGDRTPKQKMKMEVKHPRFFALFEKHGGYKTGKLKLKRPEQLQEVLDITRL
                     LLAELEKEPGGEIEEKTGKLEQLKSVLEMYGHFSGINRKVQLTYYPHGVKASNEGQDP
                     QRETLAPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQGDYAGFPGCGLLRLHS
                     TFRHDLKIYASDEGRVQMTAAAFAKGLLALEGELTPILVQMVKSANMNGLLDSDGDSL
                     SSCQHRVKARLHHILQQDAPFGPEDYDQLAPTRSTSLLNSMTIIQNPVKVCDQVFALI
                     ENLTHQIRERMQDPRSVDLQLYHSETLELMLQRWSKLERDFRQKSGRYDISKIPDIYD
                     CVKYDVQHNGSLGLQGTAELLRLSKALADVVIPQEYGISREEKLEIAVGFCLPLLRKI
                     LLDLQRTHEDESVNKLHPLYSRGVLSPGRHVRTRLYFTSESHVHSLLSVFRYGGLLDE
                     TQDAQWQRALDYLSAISELNYMTQIVIMLYEDNTQDPLSEERFHVELHFSPGVKGVEE
                     EGSAPAGCGFRPASSENEEMKTNQGSMENLCPGKASDEPDRALQTSPQPPEGPGLPRR
                     SPLIRNRKAGSMEVLSETSSSRPGGYRLFSSSRPPTEMKQSGLGSQCTGLFSTTVLGG
                     SSSAPNLQDYARSHGKKLPPASLKHRDELLFVPAVKRFSVSFAKHPTNGFEGCSMVPT
                     IYPLETLHNALSLRQVSEFLSRVCQRHTDAQAQASAALFDSMHSSQASDNPFSPPRTL
                     HSPPLQLQQRSEKPPWYSSGPSSTVSSAGPSSPTTVDGNSQFGFSDQPSLNSHVAEEH
                     QGLGLLQETPGSGAQELSIEGEQELFEPNQSPQVPPMETSQPYEEVSQPCQEVPDISQ
                     PCQDISEALSQPCQKVPDISQQCQENHDNGNHTCQEVPHISQPCQKSSQLCQKVSEEV
                     CQLCLENSEEVSQPCQGVSVEVGKLVHKFHVGVGSLVQETLVEVGSPAEEIPEEVIQP
                     YQEFSVEVGRLAQETSAINLLSQGIPEIDKPSQEFPEEIDLQAQEVPEEIN"
     exon            332..527
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            528..618
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            619..704
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            705..859
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            860..961
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1123
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1245
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1246..1347
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1348..1434
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1435..1510
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1620
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1703
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1835
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1957
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1958..2140
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2141..2282
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2283..2506
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2507..2634
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2635..2749
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2750..2857
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2858..2974
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2975..3139
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3140..3230
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3231..3356
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3357..3419
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3420..3557
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3558..3671
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3672..5673
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5658..5663
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /note="hexamer: TATAAA"
     polyA_site      5673
                     /gene="PPIP5K1"
                     /gene_synonym="HISPPD2A; hsVIP1; IP6K; IPS1; VIP1"
                     /note="major polyA site"
ORIGIN      
        1 attcgtgacc cggagctgtg cgaggagagt accgaggagg gggtcggcgg gagaggagat
       61 aggactcaag agtagccttc ctcgaggacc tgcctttccc atttgctgcc tgaagttaat
      121 gtttcttgct ggccaaatca gggacatgcc ggcattagcg ggatgagtgg gtgttccggc
      181 agggatgtgg tcattgacgg ccagtgaggg cgagagtacc acggcccact tcttccttgg
      241 agctggagat gaggggctgg gcacccgtgg aataggcatg aggccagaag agagtgacag
      301 cgagctcctt gaggatgagg aggatgaagt gcctcctgaa cctcagatca ttgttggcat
      361 ctgtgccatg accaagaaat ccaagtccaa gccaatgact caaatcctag agcgactctg
      421 cagatttgac tacctgactg ttgtcattct gggagaagat gtaatcctta atgaacctgt
      481 ggaaaactgg ccatcctgcc actgcctcat ctctttccac tccaaaggct ttcctctgga
      541 caaagctgtt gcttactcca agcttcgaaa cccctttctt atcaatgatc tggccatgca
      601 gtattacatc caagatagga gggaggtgta ccggatcctg caggaagagg gtattgatct
      661 gcctcgatat gctgtgctca accgtgatcc tgcccggcct gaggaatgca acctgataga
      721 aggtgaagac caagtagagg tcaatggagc tgtctttccc aagccctttg tggagaagcc
      781 agtgagtgca gaagaccaca atgtttacat ctactacccc agctcagctg gaggaggaag
      841 ccagcgtctc tttcgtaaga ttggcagccg aagcagtgtt tactctcctg agagcagcgt
      901 ccgaaagacg gggtcgtaca tctatgagga gtttatgcca acagatggca cagatgtcaa
      961 ggtgtataca gtggggccag attatgccca tgctgaagct agaaaatctc cagctttgga
     1021 tgggaaggtt gaacgagaca gtgaggggaa agagattcga tatccagtca tgctgactgc
     1081 catggaaaag ctggtggcca ggaaagtctg cgtagctttc aagcaaacag tttgtggatt
     1141 tgaccttctt cgtgccaatg gtcattcctt tgtgtgtgat gtcaatggct ttagttttgt
     1201 caagaactcg atgaaatact acgatgactg tgccaagatt ctggggaaca ccataatgcg
     1261 ggagcttgcc ccacagttcc agattccatg gtccatcccc acggaggctg aggacattcc
     1321 cattgttccc accacatctg gcactatgat ggaacttcgt tgtgtcattg caattattcg
     1381 tcatggggat cgtactccca agcagaagat gaagatggaa gtgaaacacc caaggttttt
     1441 tgctctgttt gaaaaacatg gtggctacaa gacagggaaa ttaaaactca agcgacctga
     1501 gcagctccag gaggtgctgg atatcacaag gctgttgttg gctgaactgg agaaagaacc
     1561 aggtggtgag atcgaggaga agactggaaa actagagcag ctgaagtctg tactggagat
     1621 gtatggtcac ttctcaggta taaaccggaa ggtacaattg acttactacc ctcatggagt
     1681 aaaagcttct aatgaggggc aagatccaca gagggaaact ctggccccat ctctgttgct
     1741 ggtactgaag tggggtggag aactgactcc tgctggccgt gttcaggctg aggagctggg
     1801 gcgagctttt cgctgcatgt accctggagg acagggtgac tatgctggct tccctggttg
     1861 tgggctgctt cgtctccata gcactttccg ccacgatctc aagatctatg cctctgatga
     1921 gggtcgtgtt cagatgactg ctgctgcctt cgccaagggc cttctggctc tagaagggga
     1981 gctgacaccc attttggtgc aaatggtgaa gagtgccaac atgaatgggc tactggacag
     2041 cgatggggat tccttgagca gctgccagca ccgggtgaag gctcggctgc accatattct
     2101 acagcaggat gcgccctttg gccctgagga ctacgatcag ctggctccca ccagaagtac
     2161 ttccctgctc aactccatga ctatcatcca gaatcctgtg aaggtctgtg atcaggtatt
     2221 tgccctgatc gaaaacctca cccaccagat ccgggaacga atgcaggacc ccaggtctgt
     2281 agacctgcag ctctaccaca gtgagacact agagctaatg ctacagcgtt ggagcaagct
     2341 ggagcgtgac tttcgacaga agagtgggcg ctatgatatc agtaagatcc ctgacatcta
     2401 tgactgtgtc aagtatgatg tgcagcacaa tgggagtctg ggacttcaag gcacagcaga
     2461 gttgctccgt ctctctaagg cactggctga tgtggtcatt ccccaggagt acgggatcag
     2521 tcgggaggag aaactggaaa ttgctgtggg cttctgtctt ccactgttgc ggaagatact
     2581 acttgacctg cagagaaccc acgaggatga gtctgtcaac aagctgcatc ccctgtactc
     2641 ccgaggcgtg ctctccccag gtcgccacgt tcgaacgcgt ctctatttca ccagtgagag
     2701 ccatgtccac tccctgctca gtgtcttccg ttatggagga cttcttgatg agacccagga
     2761 tgcacaatgg cagcgagctt tggattatct tagtgccatc tcagagctta actacatgac
     2821 ccagattgtc atcatgcttt atgaggacaa cacacaggat cccttatcag aggaacggtt
     2881 ccatgtggag ctacacttca gccccggagt gaaaggtgtt gaggaagaag gcagtgcccc
     2941 ggctggctgt ggattccgtc cagcctcttc tgagaatgag gagatgaaaa ccaaccaagg
     3001 cagtatggag aacctgtgtc caggaaaggc atcagatgaa ccagaccggg cattgcagac
     3061 ttcaccccag cctcctgagg gccctggcct tccgaggaga tcacccctca ttcgtaaccg
     3121 aaaagctggt tccatggagg tactttctga gacttcatcc tcgaggcctg gtggctaccg
     3181 gctcttttca tcttcacggc cacccacaga aatgaagcag agtggcctag gctcacagtg
     3241 cacagggctg ttcagcacca cagtgctggg tggctcctcc agtgccccga atcttcagga
     3301 ctacgcccgc agccatggca aaaagctacc acctgccagt ctgaagcacc gagatgagct
     3361 cttgtttgtc ccggccgtaa aacgattttc tgtgtcgttt gcaaagcatc cgactaacgg
     3421 gtttgaaggg tgttccatgg tgcctaccat ctaccctctg gaaacactgc ataatgccct
     3481 ttccctacgt caagtgagtg aattcttgag tagagtctgc cagcgccaca ctgatgccca
     3541 ggcacaggca tctgcagccc tctttgattc catgcacagc agccaggcct cagataaccc
     3601 attttctcca ccacgtactc ttcattcacc tcccctgcaa ctccagcagc gctctgagaa
     3661 gcccccttgg tacagcagtg gcccttctag cactgtgtcc agtgctggtc cttcttcccc
     3721 tactacagta gatggtaact cccaatttgg cttcagtgat caaccctccc taaattcaca
     3781 cgtggctgaa gaacatcaag gccttgggct gctccaggag acccctggga gtggagcaca
     3841 agagctctcc atagaagggg agcaagagct ttttgaacca aatcagtccc cacaggtgcc
     3901 acctatggaa accagccagc catacgagga ggtcagccag ccatgtcagg aggtccctga
     3961 catcagccag ccatgccagg acatttctga ggcgctcagc cagccatgtc agaaggtccc
     4021 tgacatcagc cagcaatgcc aggagaacca tgacaatggt aaccacacat gccaggaggt
     4081 ccctcacatc agccagccat gccagaagtc cagccaactg tgccagaaag tctctgagga
     4141 agtttgccag ctatgtctgg agaactccga ggaggtcagc cagccatgcc agggggtctc
     4201 tgtggaggtt ggcaagctgg tccataagtt ccatgtaggg gttggtagct tggtccagga
     4261 aacccttgta gaagttggca gcccagctga agagatccct gaggaggtca tccagccata
     4321 ccaggagttc tctgtggagg ttggcaggct ggcccaggag acttctgcga tcaatctgtt
     4381 atctcagggc atccctgaga ttgataaacc atcccaagag ttccctgagg agattgatct
     4441 gcaggcccag gaggtccctg aggagataaa ttagaagtcc tgggtggtcc ctgaagtgat
     4501 tgatcagctg cctggagagg gtattcctca agcccagcat ccatctggtg atccaaaccc
     4561 tcagagccag tctctagccc atgaccagca ctcacccctt ccaccagcaa catgtgatta
     4621 attttctcat tagtggtatc acactatacc agccatttga gccagcaacc ttttctgttg
     4681 gctaactcac tggccagctc tcaccagcgg tgtctgggga agtagttctc tttgtatgaa
     4741 gcatacctgt gccagagctg tggttgagga ggagccagtt ttaggttcga agaagccatt
     4801 ggctcctcac ttagccatta gacttgaata ggatttcctt ggggtgggtt ggtctgtcat
     4861 tacccagcct tctctgagca tcctaggaaa tcacagattg ttaaaggaaa tgccgcttca
     4921 ctgctgaaga caccatctgg cgacagcaaa tgcaaaagag gggactctag ggtcttcact
     4981 tttctgggga aatgttcacg acttctcaag gtacgcttag accatatgtg cattcagggg
     5041 actcttgtct ttgccagcac tcctcaggtg agcccgggat ggctatctga aatgtctgga
     5101 aaatagagtc ccttccccaa acatctgaca tggccactaa atctttagac ttgccttata
     5161 gatccttcca tatcatcccc aagtgcccct tctgcctcag tcctgttcct ctgggcaata
     5221 gctctaggga aaagtaggta ttagactgct gtgcaaaatt cagagcaact acttaaaatg
     5281 ctctagagga ttcttagcca gtctgtgaaa gtgggcctcc tctgtggtgg ggctgtttgg
     5341 cttaggagat gctgtagtag tagagataat caggttctat tttaagcaat cagcagagat
     5401 caatattgta cagatacaag caaaggttta ataaatatat atatatatat ttatttctgt
     5461 agttgtgcca gggacagact ggccaaagac caaacactgc agggtcccca agaaatttgt
     5521 ccttatatca ttgtgtctga gggccagata tgattatgaa gctttttcca aagatccagg
     5581 gatgggaatg ggagtgggta ggaggggtaa tgcggtcatt ggagtcgggg gctggaacat
     5641 tatgagtgct caataaatat aaactaatga gaa
//
