LOCUS       NM_003622               5983 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens PPFIA binding protein 1 (PPFIBP1), transcript variant
            1, mRNA.
ACCESSION   NM_003622 XM_005253513
VERSION     NM_003622.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Waqas,A., Liaqat,R., Shaheen,S., Khan,A.Z., Mujahid, Habib,A.H.,
            Binothman,N., Aljadani,M., Zehri,Z., Shaheen,S., Alkathiri,A.,
            Naz,R., Umair,M. and Abbas,S.
  TITLE     A novel homozygous truncating variant in PPFIBP1 further delineates
            PPFIBP1-associated neurodevelopmental disorder
  JOURNAL   Int J Dev Neurosci 83 (2), 191-200 (2023)
   PUBMED   36527195
  REMARK    GeneRIF: A novel homozygous truncating variant in PPFIBP1 further
            delineates PPFIBP1-associated neurodevelopmental disorder.
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   3  (bases 1 to 5983)
  AUTHORS   Kim,D.K., Weller,B., Lin,C.W., Sheykhkarimli,D., Knapp,J.J.,
            Dugied,G., Zanzoni,A., Pons,C., Tofaute,M.J., Maseko,S.B.,
            Spirohn,K., Laval,F., Lambourne,L., Kishore,N., Rayhan,A.,
            Sauer,M., Young,V., Halder,H., la Rosa,N.M., Pogoutse,O.,
            Strobel,A., Schwehn,P., Li,R., Rothballer,S.T., Altmann,M.,
            Cassonnet,P., Cote,A.G., Vergara,L.E., Hazelwood,I., Liu,B.B.,
            Nguyen,M., Pandiarajan,R., Dohai,B., Coloma,P.A.R., Poirson,J.,
            Giuliana,P., Willems,L., Taipale,M., Jacob,Y., Hao,T., Hill,D.E.,
            Brun,C., Twizere,J.C., Krappmann,D., Heinig,M., Falter,C., Aloy,P.,
            Demeret,C., Vidal,M., Calderwood,M.A., Roth,F.P. and
            Falter-Braun,P.
  TITLE     A proteome-scale map of the SARS-CoV-2-human contactome
  JOURNAL   Nat Biotechnol 41 (1), 140-149 (2023)
   PUBMED   36217029
REFERENCE   4  (bases 1 to 5983)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 5983)
  AUTHORS   Dong,C., Li,X., Yang,J., Yuan,D., Zhou,Y., Zhang,Y., Shi,G.,
            Zhang,R., Liu,J., Fu,P. and Sun,M.
  TITLE     PPFIBP1 induces glioma cell migration and invasion through
            FAK/Src/JNK signaling pathway
  JOURNAL   Cell Death Dis 12 (9), 827 (2021)
   PUBMED   34480020
  REMARK    GeneRIF: PPFIBP1 induces glioma cell migration and invasion through
            FAK/Src/JNK signaling pathway.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5983)
  AUTHORS   Benzinger,A., Muster,N., Koch,H.B., Yates,J.R. 3rd and Hermeking,H.
  TITLE     Targeted proteomic analysis of 14-3-3 sigma, a p53 effector
            commonly silenced in cancer
  JOURNAL   Mol Cell Proteomics 4 (6), 785-795 (2005)
   PUBMED   15778465
REFERENCE   7  (bases 1 to 5983)
  AUTHORS   Jin,J., Smith,F.D., Stark,C., Wells,C.D., Fawcett,J.P.,
            Kulkarni,S., Metalnikov,P., O'Donnell,P., Taylor,P., Taylor,L.,
            Zougman,A., Woodgett,J.R., Langeberg,L.K., Scott,J.D. and Pawson,T.
  TITLE     Proteomic, functional, and domain-based analysis of in vivo 14-3-3
            binding proteins involved in cytoskeletal regulation and cellular
            organization
  JOURNAL   Curr Biol 14 (16), 1436-1450 (2004)
   PUBMED   15324660
REFERENCE   8  (bases 1 to 5983)
  AUTHORS   Meek,S.E., Lane,W.S. and Piwnica-Worms,H.
  TITLE     Comprehensive proteomic analysis of interphase and mitotic
            14-3-3-binding proteins
  JOURNAL   J Biol Chem 279 (31), 32046-32054 (2004)
   PUBMED   15161933
REFERENCE   9  (bases 1 to 5983)
  AUTHORS   Kriajevska,M., Fischer-Larsen,M., Moertz,E., Vorm,O.,
            Tulchinsky,E., Grigorian,M., Ambartsumian,N. and Lukanidin,E.
  TITLE     Liprin beta 1, a member of the family of LAR transmembrane tyrosine
            phosphatase-interacting proteins, is a new target for the
            metastasis-associated protein S100A4 (Mts1)
  JOURNAL   J Biol Chem 277 (7), 5229-5235 (2002)
   PUBMED   11836260
  REMARK    GeneRIF: new molecular target of the S100A4 protein, liprin beta1
REFERENCE   10 (bases 1 to 5983)
  AUTHORS   Serra-Pages,C., Medley,Q.G., Tang,M., Hart,A. and Streuli,M.
  TITLE     Liprins, a family of LAR transmembrane protein-tyrosine
            phosphatase-interacting proteins
  JOURNAL   J Biol Chem 273 (25), 15611-15620 (1998)
   PUBMED   9624153
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC068794.25, AC087257.13 and
            AC009509.7.
            
            On Nov 23, 2018 this sequence version replaced NM_003622.3.
            
            Summary: The protein encoded by this gene is a member of the LAR
            protein-tyrosine phosphatase-interacting protein (liprin) family.
            Liprins interact with members of LAR family of transmembrane
            protein tyrosine phosphatases, which are known to be important for
            axon guidance and mammary gland development. It has been proposed
            that liprins are multivalent proteins that form complex structures
            and act as scaffolds for the recruitment and anchoring of LAR
            family of tyrosine phosphatases. This protein was found to interact
            with S100A4, a calcium-binding protein related to tumor
            invasiveness and metastasis. In vitro experiment demonstrated that
            the interaction inhibited the phosphorylation of this protein by
            protein kinase C and protein kinase CK2. Alternatively spliced
            transcript variants encoding distinct isoforms have been reported.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) has multiple differences in
            the coding sequence compared to variant 2. The resulting isoform
            (1) has the same N- and C-termini but is 6 aa shorter compared to
            isoform 2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.102193.1, AF034802.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000228425.11/ ENSP00000228425.6
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-160               AC068794.25        119371-119530
            161-248             AC087257.13        145344-145431
            249-347             AC009509.7         47266-47364
            348-553             AC009509.7         48814-49019
            554-640             AC009509.7         59966-60052
            641-754             AC009509.7         61633-61746
            755-886             AC009509.7         63914-64045
            887-979             AC009509.7         68626-68718
            980-1094            AC009509.7         70520-70634
            1095-1127           AC009509.7         72720-72752
            1128-1189           AC009509.7         74788-74849
            1190-1274           AC009509.7         78266-78350
            1275-1429           AC009509.7         81070-81224
            1430-1545           AC009509.7         85335-85450
            1546-1602           AC009509.7         86331-86387
            1603-1663           AC009509.7         87671-87731
            1664-1693           AC009509.7         88096-88125
            1694-1865           AC009509.7         90332-90503
            1866-1898           AC009509.7         90968-91000
            1899-2049           AC009509.7         93393-93543
            2050-2178           AC009509.7         93837-93965
            2179-2329           AC009509.7         95450-95600
            2330-2441           AC009509.7         96291-96402
            2442-2530           AC009509.7         96519-96607
            2531-2653           AC009509.7         101289-101411
            2654-2779           AC009509.7         102202-102327
            2780-2968           AC009509.7         102919-103107
            2969-3148           AC009509.7         105653-105832
            3149-3214           AC009509.7         106495-106560
            3215-5983           AC009509.7         106700-109468
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p11.23-p11.22"
     gene            1..5983
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /note="PPFIA binding protein 1"
                     /db_xref="GeneID:8496"
                     /db_xref="HGNC:HGNC:9249"
                     /db_xref="MIM:603141"
     exon            1..160
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            161..248
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            249..347
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     CDS             284..3301
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     liprin related protein; liprin-beta-1; protein-tyrosine
                     phosphatase receptor-type f polypeptide-interacting
                     protein-binding protein 1; PTPRF-interacting
                     protein-binding protein 1; PTPRF interacting protein,
                     binding protein 1 (liprin beta 1)"
                     /codon_start=1
                     /product="liprin-beta-1 isoform 1"
                     /protein_id="NP_003613.4"
                     /db_xref="CCDS:CCDS8713.1"
                     /db_xref="GeneID:8496"
                     /db_xref="HGNC:HGNC:9249"
                     /db_xref="MIM:603141"
                     /translation="MMSDASDMLAAALEQMDGIIAGSKALEYSNGIFDCQSPTSPFMG
                     SLRALHLVEDLRGLLEMMETDEKEGLRCQIPDSTAETLVEWLQSQMTNGHLPGNGDVY
                     QERLARLENDKESLVLQVSVLTDQVEAQGEKIRDLEFCLEEHREKVNATEEMLQQELL
                     SRTSLETQKLDLMAEISNLKLKLTAVEKDRLDYEDKFRDTEGLIQEINDLRLKVSEMD
                     SERLQYEKKLKSTKDELASLKEQLEEKESEVKRLQEKLVCKMKGEGVEIVDRDENFKK
                     KLKEKNIEVQKMKKAVESLMAANEEKDRKIEDLRQCLNRYKKMQDTVVLAQGKKGKDG
                     EYEELLNSSSISSLLDAQGFSDLEKSPSPTPVMGSPSCDPFNTSVPEEFHTTILQVSI
                     PSLLPATVSMETSEKSKLTPKPETSFEENDGNIILGATVDTQLCDKLLTSSLQKSSSL
                     GNLKKETSDGEKETIQKTSEDRAPAESRPFGTLPPRPPGQDTSMDDNPFGTRKVRSSF
                     GRGFFKIKSNKRTASAPNLDRKRSASAPTLAETEKETAEHLDLAGASSRPKDSQRNSP
                     FQIPPPSPDSKKKSRGIMKLFGKLRRSQSTTFNPDDMSEPEFKRGGTRATAGPRLGWS
                     RDLGQSNSDLDMPFAKWTKEQVCNWLMEQGLGSYLNSGKHWIASGQTLLQASQQDLEK
                     ELGIKHSLHRKKLQLALQALGSEEETNHGKLDFNWVTRWLDDIGLPQYKTQFDEGRVD
                     GRMLHYMTVDDLLSLKVVSVLHHLSIKRAIQVLRINNFEPNCLRRRPSDENTIAPSEV
                     QKWTNHRVMEWLRSVDLAEYAPNLRGSGVHGGLMVLEPRFNVETMAQLLNIPPNKTLL
                     RRHLATHFNLLIGAEAQHQKRDAMELPDYVLLTATAKVKPKKLAFSNFGNLRKKKQED
                     GEEYVCPMELGQASGSASKKGFKPGLDMRLYEEDDLDRLEQMEDSEGTVRQIGAFSEG
                     INNLTHMLKEDDMFKDFAARSPSASITDEDSNV"
     exon            348..553
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            554..640
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            641..754
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            755..886
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            887..979
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1094
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1127
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1189
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1190..1274
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1429
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1545
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1602
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1603..1663
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1664..1693
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1694..1865
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..1898
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..2049
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2050..2178
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2179..2329
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2330..2441
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2442..2530
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2531..2653
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2654..2779
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2780..2968
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2969..3148
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3149..3214
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3215..5983
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5958..5963
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /note="hexamer: AATAAA"
     polyA_site      5983
                     /gene="PPFIBP1"
                     /gene_synonym="hSGT2; hSgt2p; L2; NEDSMBA; SGT2"
                     /note="major polyA site"
ORIGIN      
        1 ggattcctga catggtgtag tgcaggcagg gtggggaaag gacggggaag gactcgtgtg
       61 ctgcgagctg gcggccgggc cggagtgctg gggctttgaa ctccgagagg aggtggacca
      121 gaacttttgg aactagtgcc ggcggctctc caccccccag tataaaagaa cgtgtggatc
      181 actttgctga gtacatccaa gatttgaaga actgaaataa atcagcttta aacctgcttt
      241 ttaaaaatat ctgggttgga atttgcccct gacaaataat aaaatgatga gtgatgcaag
      301 tgacatgttg gctgcagcgt tggagcagat ggatggtatc atagcaggtt ctaaggctct
      361 ggaatattcc aatgggattt ttgattgcca atctcccacc tctccattca tgggaagttt
      421 gcgagctctg caccttgtgg aagacctgcg tggattgtta gagatgatgg aaacagatga
      481 gaaagaaggc ttgagatgcc agatcccaga ttcaacagca gaaacgcttg ttgaatggct
      541 tcagagtcaa atgacaaatg gacacctacc agggaacgga gatgtgtatc aagaaaggct
      601 ggcacgttta gaaaatgata aagaatccct cgttcttcag gtaagtgtgt taacagacca
      661 ggtggaggct cagggagaga agattcgaga tttggagttt tgtcttgaag agcacagaga
      721 gaaggtgaat gccacagaag aaatgctgca gcaggagctt ctaagtagga catccttaga
      781 aactcagaag ttggatctga tggctgaaat atctaacttg aagttgaaac tgacagctgt
      841 agagaaggac agattggatt atgaagataa gttcagagac acagaggggc tgattcagga
      901 gatcaatgat ttgaggttaa aagttagtga aatggacagt gagagacttc agtatgaaaa
      961 aaagcttaaa tcaaccaaag atgaactggc atctttaaaa gaacaactag aagaaaagga
     1021 atctgaagta aaaaggctac aagaaaaatt ggtttgcaag atgaaaggag aaggggttga
     1081 aattgttgat agagatgaaa attttaaaaa gaagctcaaa gaaaaaaaca tcgaagtaca
     1141 aaaaatgaaa aaagctgtgg agtccttgat ggcagcaaat gaagaaaagg atcggaaaat
     1201 agaagatctt cgacagtgcc tgaacaggta caagaaaatg caagacacgg tggtactggc
     1261 ccaaggtaaa aaaggcaaag atggagaata tgaagagctg ctcaattcca gttccatctc
     1321 ctctttgctg gatgcacagg gtttcagtga tctggagaaa agtccatcac ccactccagt
     1381 aatgggatct cccagttgtg acccatttaa cacaagtgtt cccgaagagt tccatactac
     1441 catcttgcaa gtttccatcc cttcattatt gccagcaact gtaagcatgg aaacttctga
     1501 aaaatcaaag ttgactccta agccagagac ttcatttgaa gaaaatgatg gaaacataat
     1561 ccttggtgcc actgttgata cccaactgtg tgataaactt ttaacttcaa gtctgcagaa
     1621 gtccagcagc ctgggcaatc tgaagaaaga gacatctgat ggggaaaagg aaactattca
     1681 gaagacttca gaggacagag ctccggcaga aagcaggcca tttgggaccc ttcctcccag
     1741 gcccccaggg caggacacct ccatggatga caaccccttc ggcactcgaa aagtcagatc
     1801 ttcctttggc cggggctttt ttaaaatcaa aagtaacaag agaacagcaa gtgcaccaaa
     1861 cttagatcgt aaacgaagtg ccagtgcacc caccctagct gaaacagaaa aagagacagc
     1921 agagcaccta gatctggctg gtgcttcttc tcggccaaaa gattcacaga ggaacagtcc
     1981 cttccagata ccgcctccat ctccagattc caaaaagaaa tccagaggta tcatgaaact
     2041 ctttggaaaa cttaggagaa gtcaatcaac tacattcaac ccagatgaca tgtctgagcc
     2101 tgaattcaaa agaggaggga caagggcaac cgcggggccc cgattaggtt ggtctcgaga
     2161 cttgggacag tctaacagtg acttggatat gccatttgcc aagtggacca aggagcaggt
     2221 ttgcaattgg ctgatggaac agggcttggg ctcgtacctg aattctggca agcactggat
     2281 tgcatctggc caaacgcttt tgcaggcttc tcaacaagat ctagagaagg aacttggaat
     2341 caagcattca cttcatcgaa agaaactcca gctagcactc caagccctgg gatctgaaga
     2401 agaaaccaat catgggaagc tggatttcaa ctgggtcact agatggttgg atgacattgg
     2461 cctccctcaa tataagaccc agtttgatga aggacgggtt gatggtcgaa tgctacatta
     2521 catgactgtt gatgacttac tgtctctgaa ggttgtaagt gtgctacacc atctcagtat
     2581 caaaagggcc atccaggtcc tgaggatcaa taactttgaa ccaaactgtc tacggaggcg
     2641 gccatctgat gagaatacca tcgccccatc agaagttcag aagtggacta accatcgagt
     2701 gatggagtgg ctgcgctccg tggacttggc agaatatgcg cccaatctca gaggcagtgg
     2761 tgtccatggt gggctcatgg ttctagagcc tcgttttaac gtagaaacaa tggctcagtt
     2821 attgaacatc ccacccaata agactttgct gcgaagacat ttggccactc atttcaacct
     2881 tctgattggg gctgaggcac agcaccagaa gcgagatgcc atggagctgc cggattatgt
     2941 acttctaaca gctactgcca aagtgaagcc aaagaaactt gcctttagca attttgggaa
     3001 tttgagaaag aagaaacagg aagatggtga agaatatgtt tgtccaatgg aattgggaca
     3061 ggcatcagga agtgcatcta agaaaggatt taaacctggt ttggatatgc gcctgtatga
     3121 ggaagatgat ttggaccggt tagagcagat ggaagattca gaagggacag tgagacagat
     3181 aggtgcattc tctgaaggca tcaacaatct gacgcacatg ttaaaagaag atgacatgtt
     3241 taaagatttt gctgcccgtt cccccagtgc cagcattaca gatgaagact caaacgtttg
     3301 accgtagcac ctggatgaac attaggagtg cttagtcttt tttctacttg cttttccaaa
     3361 cactcacagt atatacaaca ggcagcggat tgtctattgt ttgttgttcc aacttctgct
     3421 gtcgagaagt ttaaacagaa agcaggagta atgtgccgat tctgaagttg ccacaaaaaa
     3481 taagacactg gtgaatgaga gtataattgt ttttcttcta tttaatgtaa aaatctgtga
     3541 tatattatat ttaaagtgtt gcatttaaga tgagtatttt accagagtgt ttccattcat
     3601 atccgcggta tggaggattt gaggaacagt aaccaggatg tgaatgattt tgttacatca
     3661 gtgttcactg tagccaccta agtaggacat tatatgattt cagaatcaat atgtggaact
     3721 tctttaagca ttcagtgtgc ccactaaatg ccagccacac ctccacttgc ctcttattgt
     3781 cttattttta tatatttttc taaatatatg tatatataca gtacatagaa aatagaactt
     3841 ttattttgtg acctaaggac gatggtgaaa agatcacgtt ttcaaaacaa tctggtgatc
     3901 agaatgttca tataccagct ggtttctgaa gaggtcagaa tgatctttct ccatactgac
     3961 ttttaacaat gttgatcatt gaggctaaat taatatatat gaaatattcc tttttgatga
     4021 caccacaaaa ttgttgaaca gtttaagaat ttcaacctta atcttggatc cctttacctc
     4081 atatggaaga acttgaggga cattagtata ctttttttta agatggagtc ttgctctgtc
     4141 acccaggttg gagtgccatg gcatgatctt ggctcactgc aacctccacc tcctgggtca
     4201 agccattctg cttcagcccc aagtaggtgg gactccaggc atgcaccacc atgcctggct
     4261 aatttttgca tttttagtag agacagggtt tcaccatatt ggccaggctg ggactcgaac
     4321 tcctgacctt gtgatctgcc cgcctcagcc tcccaaagta ctgggattat aggcatgagc
     4381 caccacgccc agcctgttat ttttttatta ttattgtttt tttttagtga cagagtctca
     4441 ttctgttgcc catgctggag tgcagtggcg tgatcatagc tcactgcagc cttgaattcc
     4501 taggctccag tgatcctctc acctcagctt ccctaatagc taggattaca ggtgtgtggc
     4561 ctcccacccc accccaccct tcacacctgg ctgatttttc aaaaagtttt tttgtagaaa
     4621 cagggtctca ccatgttgtc cagcctggtc tcaaactcct gtcctcaagt gatcctccca
     4681 cctcagcctt tcaaagtgct ggaattacag gtgtgagcca ctctgcctgg cctaccacta
     4741 acttgaatac attcagaatc acctcctctc cccaaaattt gtagaaatag tttttgagga
     4801 agccaaaagc aaagcagaaa cctttacagt attgtttctt ttctctttgt taactgtgtc
     4861 attacagcaa aatactagca gtctgcctaa acatgttcat tgtacatttc tcaggctatc
     4921 aatgaatgga ggtttttaaa aagttgaata tttgtctgaa cattttattt caaagttcaa
     4981 aaaaacagag gctgcaaaat tcattttata atggctattt tgtgacgata agatgtagtt
     5041 catgtttttc tgtagcactg ggcccaaata ttctttgtaa agaaaatcgc tgcagcaaaa
     5101 actgttactg tgtttattat atttgtagaa gtattagaaa aatattctat tttttattca
     5161 gtgctgcgta attacccatg gtagccaacc ctacaaaaga caggttttca caaattgagg
     5221 tggaggtggg cggttcagta tctgccactg gacttgatta taaactgtat ttgaatatca
     5281 gtggtattat cttttaagtt gtcagcaagt taccaaggta ttcattaaag aacttgtaat
     5341 atcaaattac tatttattca taacaattga tttgatgcta ataataattt tctttaaact
     5401 ctaccattca ttatgtggta actgtattga acttacttta tttggatttt attttaatgt
     5461 gactagatgt caccacttca aaaaatcaat ttgttcttag aacctggttg aaaataccag
     5521 gaaactgtta cagacgccat tttttttttt tttgagacgg agtcttgctc tgttgcccag
     5581 gctggagtgc agtggcacaa tctcagctca ctgcaagctc cgcctcctgg gttcacgcca
     5641 ttctcccacc tcagcttccc aagcagctgg gactacaggt acctgccacc acgcctggct
     5701 aattttgttt ttgtattttt agtagagacg gggtttcacc gtgttagcca ggaaggtctc
     5761 aatctcctga cctcgtgaat cgcccccctc ggtctcccaa agtgctggga ttacaggcgt
     5821 gagccaccat gcccggccca aatatttttt attcaggatg gtataaccta actgataata
     5881 ggtaataagg ttaaattttt ttatgacgta ttttatttac aaatatcata cactgctggt
     5941 gttaccatat gaaaggaaat aaagtcaatt gataattgcc tca
//
