LOCUS       NM_003625               5674 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens PTPRF interacting protein alpha 2 (PPFIA2), transcript
            variant 1, mRNA.
ACCESSION   NM_003625
VERSION     NM_003625.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   He,Y., de Witte,L.D., Schubart,C.D., Van Gastel,W.A.,
            Koeleman,B.P.C., de Jong,S., Ophoff,R.A., Hol,E.M. and Boks,M.P.
  TITLE     Liprin alfa 2 gene expression is increased by cannabis use and
            associated with neuropsychological function
  JOURNAL   Eur Neuropsychopharmacol 29 (5), 643-652 (2019)
   PUBMED   30879928
  REMARK    GeneRIF: Liprin alfa 2 gene expression is increased by cannabis use
            and associated with neuropsychological function.
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Stucchi,R., Plucinska,G., Hummel,J.J.A., Zahavi,E.E., Guerra San
            Juan,I., Klykov,O., Scheltema,R.A., Altelaar,A.F.M. and
            Hoogenraad,C.C.
  TITLE     Regulation of KIF1A-Driven Dense Core Vesicle Transport: Ca2+/CaM
            Controls DCV Binding and Liprin-alpha/TANC2 Recruits DCVs to
            Postsynaptic Sites
  JOURNAL   Cell Rep 24 (3), 685-700 (2018)
   PUBMED   30021165
REFERENCE   3  (bases 1 to 5674)
  AUTHORS   Uehara,D.T., Hayashi,S., Okamoto,N., Mizuno,S., Chinen,Y.,
            Kosaki,R., Kosho,T., Kurosawa,K., Matsumoto,H., Mitsubuchi,H.,
            Numabe,H., Saitoh,S., Makita,Y., Hata,A., Imoto,I. and Inazawa,J.
  TITLE     SNP array screening of cryptic genomic imbalances in 450 Japanese
            subjects with intellectual disability and multiple congenital
            anomalies previously negative for large rearrangements
  JOURNAL   J Hum Genet 61 (4), 335-343 (2016)
   PUBMED   26740234
  REMARK    GeneRIF: mong the 22 cases, we identified PPFIA2 as a novel
            candidate gene forIntellect Analysis of copy-neutral loss of
            heterozygosity (CNLOH) detected one case in which the CNLOH regions
            seem to be significant. The SNP array detected a modest fraction of
            small causative CNVs, which is explained by the fact that the
            majority of causative CNVs have larger sizes, and those had been
            mostly identified in the two previous screenings.
REFERENCE   4  (bases 1 to 5674)
  AUTHORS   Rietveld,C.A., Esko,T., Davies,G., Pers,T.H., Turley,P.,
            Benyamin,B., Chabris,C.F., Emilsson,V., Johnson,A.D., Lee,J.J., de
            Leeuw,C., Marioni,R.E., Medland,S.E., Miller,M.B., Rostapshova,O.,
            van der Lee,S.J., Vinkhuyzen,A.A., Amin,N., Conley,D.,
            Derringer,J., van Duijn,C.M., Fehrmann,R., Franke,L., Glaeser,E.L.,
            Hansell,N.K., Hayward,C., Iacono,W.G., Ibrahim-Verbaas,C.,
            Jaddoe,V., Karjalainen,J., Laibson,D., Lichtenstein,P.,
            Liewald,D.C., Magnusson,P.K., Martin,N.G., McGue,M., McMahon,G.,
            Pedersen,N.L., Pinker,S., Porteous,D.J., Posthuma,D.,
            Rivadeneira,F., Smith,B.H., Starr,J.M., Tiemeier,H., Timpson,N.J.,
            Trzaskowski,M., Uitterlinden,A.G., Verhulst,F.C., Ward,M.E.,
            Wright,M.J., Davey Smith,G., Deary,I.J., Johannesson,M., Plomin,R.,
            Visscher,P.M., Benjamin,D.J., Cesarini,D. and Koellinger,P.D.
  TITLE     Common genetic variants associated with cognitive performance
            identified using the proxy-phenotype method
  JOURNAL   Proc Natl Acad Sci U S A 111 (38), 13790-13794 (2014)
   PUBMED   25201988
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 2015 Jan 27;112(4):E380. doi:
            10.1073/pnas.1424631112.. PMID: 25572966]
REFERENCE   5  (bases 1 to 5674)
  AUTHORS   Ritchie,M.D., Verma,S.S., Hall,M.A., Goodloe,R.J., Berg,R.L.,
            Carrell,D.S., Carlson,C.S., Chen,L., Crosslin,D.R., Denny,J.C.,
            Jarvik,G., Li,R., Linneman,J.G., Pathak,J., Peissig,P.,
            Rasmussen,L.V., Ramirez,A.H., Wang,X., Wilke,R.A., Wolf,W.A.,
            Torstenson,E.S., Turner,S.D. and McCarty,C.A.
  TITLE     Electronic medical records and genomics (eMERGE) network
            exploration in cataract: several new potential susceptibility loci
  JOURNAL   Mol Vis 20, 1281-1295 (2014)
   PUBMED   25352737
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5674)
  AUTHORS   Wei,Z., Zheng,S., Spangler,S.A., Yu,C., Hoogenraad,C.C. and
            Zhang,M.
  TITLE     Liprin-mediated large signaling complex organization revealed by
            the liprin-alpha/CASK and liprin-alpha/liprin-beta complex
            structures
  JOURNAL   Mol Cell 43 (4), 586-598 (2011)
   PUBMED   21855798
  REMARK    GeneRIF: Liprins can mediate assembly of target proteins into large
            protein complexes capable of regulating numerous cellular
            activities.
REFERENCE   7  (bases 1 to 5674)
  AUTHORS   Ko,J., Kim,S., Valtschanoff,J.G., Shin,H., Lee,J.R., Sheng,M.,
            Premont,R.T., Weinberg,R.J. and Kim,E.
  TITLE     Interaction between liprin-alpha and GIT1 is required for AMPA
            receptor targeting
  JOURNAL   J Neurosci 23 (5), 1667-1677 (2003)
   PUBMED   12629171
REFERENCE   8  (bases 1 to 5674)
  AUTHORS   Fujinami,K., Uemura,H., Ishiguro,H. and Kubota,Y.
  TITLE     Liprin-alpha2 gene, protein tyrosine phosphatase LAR interacting
            protein related gene, is downregulated by androgens in the human
            prostate cancer cell line LNCaP
  JOURNAL   Int J Mol Med 10 (2), 173-176 (2002)
   PUBMED   12119554
  REMARK    GeneRIF: Liprin-alpha2 expression is downregulated by androgens in
            prostate cancer cells. It might play a role in androgen-responsive
            human prostate cancer & the loss of this gene expression might be
            associated with the androgen-independence.
REFERENCE   9  (bases 1 to 5674)
  AUTHORS   Wyszynski,M., Kim,E., Dunah,A.W., Passafaro,M., Valtschanoff,J.G.,
            Serra-Pages,C., Streuli,M., Weinberg,R.J. and Sheng,M.
  TITLE     Interaction between GRIP and liprin-alpha/SYD2 is required for AMPA
            receptor targeting
  JOURNAL   Neuron 34 (1), 39-52 (2002)
   PUBMED   11931740
REFERENCE   10 (bases 1 to 5674)
  AUTHORS   Serra-Pages,C., Medley,Q.G., Tang,M., Hart,A. and Streuli,M.
  TITLE     Liprins, a family of LAR transmembrane protein-tyrosine
            phosphatase-interacting proteins
  JOURNAL   J Biol Chem 273 (25), 15611-15620 (1998)
   PUBMED   9624153
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC079408.25, AF034799.1,
            DA329958.1, DA533482.1, DA368637.1, BM661844.1, CB051855.1,
            EL947964.1 and AW304872.1.
            
            On Jun 2, 2019 this sequence version replaced NM_003625.4.
            
            Summary: The protein encoded by this gene is a member of the LAR
            protein-tyrosine phosphatase-interacting protein (liprin) family.
            Liprins interact with members of LAR family of transmembrane
            protein tyrosine phosphatases, which are known to be important for
            axon guidance and mammary gland development. It has been proposed
            that liprins are multivalent proteins that form complex structures
            and act as scaffolds for the recruitment and anchoring of LAR
            family of tyrosine phosphatases. This protein has been shown to
            bind the calcium/calmodulin-dependent serine protein kinase (MAGUK
            family) protein (also known as CASK) and proposed to regulate
            higher-order brain functions in mammals. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Sep
            2013].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF034799.1, SRR1803617.231105.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000549396.6/ ENSP00000450337.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                AC079408.25        89246-89265
            21-1300             AF034799.1         9-1288
            1301-1366           DA329958.1         258-323
            1367-1452           AF034799.1         1355-1440
            1453-1737           DA533482.1         188-472
            1738-2265           AF034799.1         1726-2253
            2266-2276           DA368637.1         200-210
            2277-4072           AF034799.1         2265-4060
            4073-4382           BM661844.1         18-327              c
            4383-4782           CB051855.1         27-426              c
            4783-5284           EL947964.1         83-584
            5285-5674           AW304872.1         44-433
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q21.31"
     gene            1..5674
                     /gene="PPFIA2"
                     /note="PTPRF interacting protein alpha 2"
                     /db_xref="GeneID:8499"
                     /db_xref="HGNC:HGNC:9246"
                     /db_xref="MIM:603143"
     exon            1..71
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            72..179
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            180..430
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     CDS             182..3955
                     /gene="PPFIA2"
                     /note="isoform a is encoded by transcript variant 1;
                     liprin-alpha-2; protein tyrosine phosphatase, receptor
                     type, f polypeptide (PTPRF), interacting protein (liprin),
                     alpha 2"
                     /codon_start=1
                     /product="liprin-alpha-2 isoform a"
                     /protein_id="NP_003616.2"
                     /db_xref="CCDS:CCDS55857.1"
                     /db_xref="GeneID:8499"
                     /db_xref="HGNC:HGNC:9246"
                     /db_xref="MIM:603143"
                     /translation="MMCEVMPTINEDTPMSQRGSQSSGSDSDSHFEQLMVNMLDERDR
                     LLDTLRETQESLSLAQQRLQDVIYDRDSLQRQLNSALPQDIESLTGGLAGSKGADPPE
                     FAALTKELNACREQLLEKEEEISELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQ
                     AQSPSGVSSEVEVLKALKSLFEHHKALDEKVRERLRVSLERVSALEEELAAANQEIVA
                     LREQNVHIQRKMASSEGSTESEHLEGMEPGQKVHEKRLSNGSIDSTDETSQIVELQEL
                     LEKQNYEMAQMKERLAALSSRVGEVEQEAETARKDLIKTEEMNTKYQRDIREAMAQKE
                     DMEERITTLEKRYLSAQRESTSIHDMNDKLENELANKEAILRQMEEKNRQLQERLELA
                     EQKLQQTMRKAETLPEVEAELAQRIAALTKAEERHGNIEERMRHLEGQLEEKNQELQR
                     ARQREKMNEEHNKRLSDTVDRLLTESNERLQLHLKERMAALEEKNVLIQESETFRKNL
                     EESLHDKERLAEEIEKLRSELDQLKMRTGSLIEPTIPRTHLDTSAELRYSVGSLVDSQ
                     SDYRTTKVIRRPRRGRMGVRRDEPKVKSLGDHEWNRTQQIGVLSSHPFESDTEMSDID
                     DDDRETIFSSMDLLSPSGHSDAQTLAMMLQEQLDAINKEIRLIQEEKESTELRAEEIE
                     NRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMG
                     VMTLPSDLRKHRRKIAVVEEDGREDKATIKCETSPPPTPRALRMTHTLPSSYHNDARS
                     SLSVSLEPESLGLGSANSSQDSLHKAPKKKGIKSSIGRLFGKKEKARLGQLRGFMETE
                     AAAQESLGLGKLGTQAEKDRRLKKKHELLEEARRKGLPFAQWDGPTVVAWLELWLGMP
                     AWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPPT
                     SRTPSGNVWVTHEEMENLAAPAKTKESEEGSWAQCPVFLQTLAYGDMNHEWIGNEWLP
                     SLGLPQYRSYFMECLVDARMLDHLTKKDLRVHLKMVDSFHRTSLQYGIMCLKRLNYDR
                     KELERRREASQHEIKDVLVWSNDRVIRWIQAIGLREYANNILESGVHGSLIALDENFD
                     YSSLALLLQIPTQNTQARQILEREYNNLLALGTERRLDESDDKNFRRGSTWRRQFPPR
                     EVHGISMMPGSSETLPAGFRLTTTSGQSRKMTTDVASSRLQRLDNSTVRTYSC"
     exon            431..484
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            485..586
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            587..751
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            752..826
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            827..943
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1165
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1312
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1447
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1531
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1663
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1664..1726
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..1818
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1819..1954
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1955..2175
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            2176..2413
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            2414..2443
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            2444..2574
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            2575..2729
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            2730..2823
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            2824..2905
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            2906..3106
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            3107..3169
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            3170..3199
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            3200..3393
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            3394..3491
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            3492..3667
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            3668..3736
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            3737..3896
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            3897..3988
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     exon            3989..5674
                     /gene="PPFIA2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5362..5367
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPFIA2"
                     /note="hexamer: AATAAA"
     polyA_site      5383
                     /gene="PPFIA2"
                     /note="major polyA site"
ORIGIN      
        1 aggagtagca gccaccgcac gaggcaagtg aggagagaag atgctgtagc gtcctcaccg
       61 gctgccagca gggaaatggt ccaggagtgc tgggtgtgag cctcccttct cctcaagccg
      121 gagactgcgg ttgtcattga tcaattgaag aagcaaggac ccgaaatcac agacattagc
      181 aatgatgtgt gaagtgatgc ccacgattaa tgaggacacc ccaatgagcc aaagggggtc
      241 ccaaagcagt ggctcggact cagactccca ttttgagcag ctgatggtga atatgctaga
      301 tgaaagggat cgtcttctag acacccttcg ggagacccag gaaagcctct cacttgccca
      361 gcaaagactt caggatgtca tctatgaccg agactcactc cagagacagc tcaattcagc
      421 cctgccacag gatatcgaat ccctaacagg agggctggct ggttctaagg gggctgatcc
      481 accggaattt gctgcactga caaaagaatt aaatgcctgc agggaacaac ttctagaaaa
      541 ggaagaagaa atctctgaac ttaaagctga aagaaacaac acaagactat tactggagca
      601 tttggagtgc cttgtgtcac gacatgaaag atcactaaga atgacggtgg taaaacggca
      661 agcccagtct ccctcaggag tatccagtga agttgaagtt ctcaaggcac tgaaatcttt
      721 gtttgagcac cacaaggcct tggatgaaaa ggtaagggag cgactgaggg tttctttaga
      781 aagagtctct gcactggaag aagaactagc tgctgctaat caggagattg ttgccttgcg
      841 tgaacaaaat gttcatatac aaagaaaaat ggcatcaagc gagggatcca cagagtcaga
      901 acatcttgaa gggatggaac ctggacagaa agtccatgag aagcgtttgt ccaatggttc
      961 tatagactca accgatgaaa ctagtcaaat agttgaacta caagaattgc ttgaaaagca
     1021 aaactatgaa atggcccaga tgaaagaacg tttagcagcc ctttcttccc gagtgggaga
     1081 ggtggaacag gaagcagaga cagcaagaaa ggatctcatt aaaacagaag aaatgaacac
     1141 caagtatcaa agggacatta gggaggccat ggcacaaaag gaagatatgg aagaaagaat
     1201 tacaaccctt gaaaagcgtt acctcagtgc tcagagagaa tctacctcca tacatgacat
     1261 gaatgataaa ctagaaaatg agttagcaaa taaagaagct atcctgcggc agatggaaga
     1321 gaaaaacaga cagttacaag aacgtcttga gctagctgaa caaaagttgc agcagaccat
     1381 gagaaaggct gaaaccttgc ctgaagtaga ggctgaactg gctcagagaa ttgcagccct
     1441 aaccaaggct gaagagagac atggaaatat tgaagaacgt atgagacatt tagagggtca
     1501 acttgaagag aagaatcaag aacttcaaag agctaggcaa agagagaaaa tgaatgagga
     1561 gcataacaag agattatcgg atacggttga tagacttctg actgaatcca atgaacgcct
     1621 acaactacac ttaaaggaaa gaatggctgc tctagaagaa aagaatgttt taattcaaga
     1681 atcagaaact ttcagaaaga atcttgaaga atctttacat gataaggaaa gattagcaga
     1741 agaaattgaa aagctgagat ctgaacttga ccaattgaaa atgagaactg gctctttaat
     1801 tgaacccaca ataccaagaa ctcatctaga cacctcagct gagttgcggt actcagtggg
     1861 atccctagtg gacagccagt ctgattacag aacaactaaa gtaataagaa gaccaaggag
     1921 aggccgcatg ggtgtgcgaa gagatgagcc aaaggtgaaa tctcttgggg atcacgagtg
     1981 gaatagaact caacagattg gagtactaag cagccaccct tttgaaagtg acactgaaat
     2041 gtctgatatt gatgatgatg acagagaaac aatttttagc tcaatggatc ttctctctcc
     2101 aagtggtcat tccgatgccc agacgctagc catgatgctt caggaacaat tggatgccat
     2161 caacaaagaa atcaggctaa ttcaggaaga aaaagaatct acagagttgc gtgctgaaga
     2221 aattgaaaat agagtggcta gtgtgagcct cgaaggcctg aatttggcaa gggtccaccc
     2281 aggtacctcc attactgcct ctgttacagc ttcatcgctg gccagttcat ctccccccag
     2341 tggacactca actccaaagc tcacccctcg aagccctgcc agggaaatgg atcggatggg
     2401 agtcatgaca ctgccaagtg atctgaggaa acatcggaga aagattgcag ttgtggaaga
     2461 agatggtcga gaggacaaag caacaattaa atgtgaaact tctcctcctc ctacccctag
     2521 agccctcaga atgactcaca ctctcccttc ttcctaccac aatgatgctc gaagtagttt
     2581 atctgtctct cttgagccag aaagcctcgg gcttggtagt gccaacagca gccaagactc
     2641 tcttcacaaa gcccccaaga agaaaggaat caagtcttca ataggacgtt tgtttggtaa
     2701 aaaagaaaaa gctcgacttg ggcagctccg aggctttatg gagactgaag ctgcagctca
     2761 ggagtccctg gggttaggca aactcggaac tcaagctgag aaggatcgaa gactaaagaa
     2821 aaagcatgaa cttcttgaag aagctcggag aaagggatta ccttttgccc agtgggatgg
     2881 gccaactgtg gtcgcatggc tagagctttg gttgggaatg cctgcgtggt acgtggcagc
     2941 ctgccgagcc aacgtgaaga gtggtgccat catgtctgct ttatctgaca ctgagatcca
     3001 gagagaaatt ggaatcagca atccactgca tcgcttaaaa cttcgattag caatccagga
     3061 gatggtttcc ctaacaagtc cttcagctcc tccaacatct cgaactcctt caggcaacgt
     3121 ttgggtgact catgaagaaa tggaaaatct tgcagctcca gcaaaaacga aagaatctga
     3181 ggaaggaagc tgggcccagt gtccggtttt tctacagacc ctggcttatg gagatatgaa
     3241 tcatgagtgg attggaaatg aatggcttcc cagcttgggg ttacctcagt acagaagtta
     3301 ctttatggaa tgcttggtag atgcaagaat gttagatcac ctaacaaaaa aagatctccg
     3361 tgtccattta aaaatggtgg atagtttcca tcgaacaagt ttacaatatg gaattatgtg
     3421 cttaaagagg ttgaattatg acagaaaaga actagaaaga agacgggaag caagccaaca
     3481 tgaaataaaa gacgtgttgg tgtggagcaa tgaccgagtt attcgctgga tacaagcaat
     3541 tggacttcga gaatatgcaa ataatatact tgagagcggt gtgcatggct cacttatagc
     3601 cctggatgaa aactttgact acagcagctt agctttatta ttacagattc caacacagaa
     3661 cacccaggca aggcagattc ttgaaagaga atacaataac ctcttggccc tgggaactga
     3721 aaggcgactg gatgaaagtg atgacaagaa cttcagacgt ggatcaacct ggagaaggca
     3781 gtttcctcct cgtgaagtac atggaatcag catgatgcct gggtcctcag aaacattacc
     3841 agctggattt aggttaacca caacctctgg gcagtcaaga aaaatgacaa cagatgttgc
     3901 ttcatcaaga ctgcagaggt tagacaactc cactgttcgc acatactcat gttgaccagc
     3961 cactcaaagg aggcagcact gacctgctat ggcgtctttt cagtctactc tacctaaagt
     4021 gcactaccat ctaagaagac gagcagtgaa aacctttgtg aaaactgaat tctaaggaaa
     4081 taatgacgtc atgacttatt aaaagctgaa aaatgtgatt tttgggggga gtcagatatt
     4141 acatttgatt agtttactac aaattgtaat aaaatgctta agtcatttga ataataaaca
     4201 tcatctacat cataaactct gtacaacaga tgcttttatg aaatgaagcc agttgttttt
     4261 catgttttat tgtaatatac taggcattta tgtattaccg tgcatttctt tttaaatgtg
     4321 taagtcttat gtaaatggat ataaatatga ttttttaaaa aataaaatat atggttcatg
     4381 gagtctcgag tgcaaacatt tgacaattcc aagtactgtt tgtattttac cattccacca
     4441 tttttacagt ttttggattg ttaatagtca aatcaatatg tttccttgaa gcatgtttca
     4501 tgcttcaacg tgtttctcct tcaagtctgt caatacttaa agctgaacaa cctgcctctg
     4561 atcatgtaaa aaagaatgat ttaacctgga accggagcca aaaatagagc tttaaaggca
     4621 atcagggatg tcctatatct ttagaaatag cactgtgatg gcttgatctc cttttcaata
     4681 caaaacaaag ccaagctgtt tacaagggtc aaaagcaatt atttaaaaat ttatattaaa
     4741 aaaccataat cttctcttgt tacctgtgga ccaagaaaaa aaaaaagata aaaaaacttc
     4801 tttactaaga accacatgtt cattcaaata tcttgaaatg ccagccagta ctggacagtg
     4861 aatgtcaacc accgcagagt ctgaggctca tttccactag agaaaacgaa acccaagata
     4921 cctttccctt ttgtcaatgt aacagggcat tagccagact catgtctaat gttataagct
     4981 tttagccgag atggccttgt tgcgacttcc ctgaagtcag ttttatcaga ggaaataaat
     5041 gaaagacaga atgattaaca tatatagtgt ataaaagtat agaagagtaa tctcttccct
     5101 gtggctttat ttggtggtgt tactgttgtt tattctttgt ttatatggga gattcaaagt
     5161 aaagcctatt taataatacc atttatctaa ctgctgattt tctgctgctt gatcttatta
     5221 agcgcaagac ctgtcattag taatttcttt tctgtatttt aatttgctat gtttgcacgt
     5281 acattacatt tgttttgatg tctattttta tttaacagat tcagtcaaaa ggtaatgata
     5341 acagaaaccc tttccattgt caataaaaaa aataataatt ccagttctct gtttaagctt
     5401 tgttttctag tcacataagg ctcgacaatt tacttaaact atcaggaaat gtgttctttg
     5461 ggtgacccaa atatttagct tcaaaattga gtctgccaca agagggagat ctaaacttgc
     5521 ttatgggagt gatcttaaaa tttttttcta aaaatgtggc ctgagtagga acaatgagaa
     5581 gtagctttct aagctcatct aaacatgggc atcttgattc atagaaatga aatagaatat
     5641 aggaactacg acattataat gccataacta gatt
//
