LOCUS       NM_002703               3682 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens phosphoribosyl pyrophosphate amidotransferase (PPAT),
            transcript variant 1, mRNA.
ACCESSION   NM_002703
VERSION     NM_002703.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Huo,F.C., Xie,M., Zhu,Z.M., Zheng,J.N. and Pei,D.S.
  TITLE     SHMT2 promotes the tumorigenesis of renal cell carcinoma by
            regulating the m6A modification of PPAT
  JOURNAL   Genomics 114 (4), 110424 (2022)
   PUBMED   35798250
  REMARK    GeneRIF: SHMT2 promotes the tumorigenesis of renal cell carcinoma
            by regulating the m6A modification of PPAT.
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3682)
  AUTHORS   Huang,H., Zhao,J., Wang,T.Y., Zhang,S., Zhou,Y., Rao,Y., Qin,C.,
            Liu,Y., Chen,Y., Xia,Z. and Feng,P.
  TITLE     Species-Specific Deamidation of RIG-I Reveals Collaborative Action
            between Viral and Cellular Deamidases in HSV-1 Lytic Replication
  JOURNAL   mBio 12 (2), e00115-21 (2021)
   PUBMED   33785613
  REMARK    GeneRIF: Species-Specific Deamidation of RIG-I Reveals
            Collaborative Action between Viral and Cellular Deamidases in HSV-1
            Lytic Replication.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3682)
  AUTHORS   Gupta,A., Sharma,P., Singh,T.P. and Sharma,S.
  TITLE     Phosphopantetheine Adenylyltransferase: A promising drug target to
            combat antibiotic resistance
  JOURNAL   Biochim Biophys Acta Proteins Proteom 1869 (2), 140566 (2021)
   PUBMED   33271445
  REMARK    GeneRIF: Phosphopantetheine Adenylyltransferase: A promising drug
            target to combat antibiotic resistance.
            Review article
REFERENCE   5  (bases 1 to 3682)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 3682)
  AUTHORS   Chen,S., Nagy,P.L. and Zalkin,H.
  TITLE     Role of NRF-1 in bidirectional transcription of the human GPAT-AIRC
            purine biosynthesis locus
  JOURNAL   Nucleic Acids Res 25 (9), 1809-1816 (1997)
   PUBMED   9108165
REFERENCE   7  (bases 1 to 3682)
  AUTHORS   Brayton,K.A., Chen,Z., Zhou,G., Nagy,P.L., Gavalas,A., Trent,J.M.,
            Deaven,L.L., Dixon,J.E. and Zalkin,H.
  TITLE     Two genes for de novo purine nucleotide synthesis on human
            chromosome 4 are closely linked and divergently transcribed
  JOURNAL   J Biol Chem 269 (7), 5313-5321 (1994)
   PUBMED   8106516
REFERENCE   8  (bases 1 to 3682)
  AUTHORS   Iwahana,H., Oka,J., Mizusawa,N., Kudo,E., Ii,S., Yoshimoto,K.,
            Holmes,E.W. and Itakura,M.
  TITLE     Molecular cloning of human amidophosphoribosyltransferase
  JOURNAL   Biochem Biophys Res Commun 190 (1), 192-200 (1993)
   PUBMED   8380692
REFERENCE   9  (bases 1 to 3682)
  AUTHORS   Zalkin,H. and Dixon,J.E.
  TITLE     De novo purine nucleotide biosynthesis
  JOURNAL   Prog Nucleic Acid Res Mol Biol 42, 259-287 (1992)
   PUBMED   1574589
  REMARK    Review article
REFERENCE   10 (bases 1 to 3682)
  AUTHORS   Stanley,W. and Chu,E.H.
  TITLE     Assignment of the gene for phosphoribosylpyrophosphate
            amidotransferase to the pter leads to q21 region of human
            chromosome 4
  JOURNAL   Cytogenet Cell Genet 22 (1-6), 228-231 (1978)
   PUBMED   752480
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB039655.1, BC004200.2,
            AB209246.1 and U00238.1.
            
            On Nov 22, 2018 this sequence version replaced NM_002703.4.
            
            Summary: The protein encoded by this gene is a member of the
            purine/pyrimidine phosphoribosyltransferase family. It is a
            regulatory allosteric enzyme that catalyzes the first step of de
            novo purine nucleotide biosythetic pathway. This gene and
            PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and
            seven of this pathway, are located in close proximity on chromosome
            4, and divergently transcribed from an intergenic region. [provided
            by RefSeq, Mar 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.695382.1,
                                           SRR1803616.225891.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264220.6/ ENSP00000264220.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-532               DB039655.1         22-553
            533-2178            BC004200.2         426-2071
            2179-3679           AB209246.1         2544-4044
            3680-3682           U00238.1           3596-3598
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q12"
     gene            1..3682
                     /gene="PPAT"
                     /gene_synonym="ATASE; GPAT; PRAT"
                     /note="phosphoribosyl pyrophosphate amidotransferase"
                     /db_xref="GeneID:5471"
                     /db_xref="HGNC:HGNC:9238"
                     /db_xref="MIM:172450"
     exon            1..266
                     /gene="PPAT"
                     /gene_synonym="ATASE; GPAT; PRAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..1692
                     /gene="PPAT"
                     /gene_synonym="ATASE; GPAT; PRAT"
                     /EC_number="2.4.2.14"
                     /note="glutamine PRPP amidotransferase; glutamine
                     phosphoribosylpyrophosphatate amidotransferase; glutamine
                     phosphoribosylpyrophosphate amidotransferase"
                     /codon_start=1
                     /product="amidophosphoribosyltransferase"
                     /protein_id="NP_002694.3"
                     /db_xref="CCDS:CCDS3505.1"
                     /db_xref="GeneID:5471"
                     /db_xref="HGNC:HGNC:9238"
                     /db_xref="MIM:172450"
                     /translation="MELEELGIREECGVFGCIASGEWPTQLDVPHVITLGLVGLQHRG
                     QESAGIVTSDGSSVPTFKSHKGMGLVNHVFTEDNLKKLYVSNLGIGHTRYATTGKCEL
                     ENCQPFVVETLHGKIAVAHNGELVNAARLRKKLLRHGIGLSTSSDSEMITQLLAYTPP
                     QEQDDTPDWVARIKNLMKEAPTAYSLLIMHRDVIYAVRDPYGNRPLCIGRLIPVSDIN
                     DKEKKTSETEGWVVSSESCSFLSIGARYYREVLPGEIVEISRHNVQTLDIISRSEGNP
                     VAFCIFEYVYFARPDSMFEDQMVYTVRYRCGQQLAIEAPVDADLVSTVPESATPAALA
                     YAGKCGLPYVEVLCKNRYVGRTFIQPNMRLRQLGVAKKFGVLSDNFKGKRIVLVDDSI
                     VRGNTISPIIKLLKESGAKEVHIRVASPPIKYPCFMGINIPTKEELIANKPEFDHLAE
                     YLGANSVVYLSVEGLVSSVQEGIKFKKQKEKKHDIMIQENGNGLECFEKSGHCTACLT
                     GKYPVELEW"
     exon            267..333
                     /gene="PPAT"
                     /gene_synonym="ATASE; GPAT; PRAT"
                     /inference="alignment:Splign:2.1.0"
     exon            334..540
                     /gene="PPAT"
                     /gene_synonym="ATASE; GPAT; PRAT"
                     /inference="alignment:Splign:2.1.0"
     exon            541..653
                     /gene="PPAT"
                     /gene_synonym="ATASE; GPAT; PRAT"
                     /inference="alignment:Splign:2.1.0"
     exon            654..799
                     /gene="PPAT"
                     /gene_synonym="ATASE; GPAT; PRAT"
                     /inference="alignment:Splign:2.1.0"
     exon            800..872
                     /gene="PPAT"
                     /gene_synonym="ATASE; GPAT; PRAT"
                     /inference="alignment:Splign:2.1.0"
     exon            873..1024
                     /gene="PPAT"
                     /gene_synonym="ATASE; GPAT; PRAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1025..1152
                     /gene="PPAT"
                     /gene_synonym="ATASE; GPAT; PRAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1374
                     /gene="PPAT"
                     /gene_synonym="ATASE; GPAT; PRAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1495
                     /gene="PPAT"
                     /gene_synonym="ATASE; GPAT; PRAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..3682
                     /gene="PPAT"
                     /gene_synonym="ATASE; GPAT; PRAT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3654..3659
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPAT"
                     /gene_synonym="ATASE; GPAT; PRAT"
                     /note="hexamer: GATAAA"
     polyA_site      3682
                     /gene="PPAT"
                     /gene_synonym="ATASE; GPAT; PRAT"
                     /note="major polyA site"
ORIGIN      
        1 agtagctggg gccaccctcg ggaaggaaga gcctgtcgcg agcgcgagct tctgagctcg
       61 acgggccgag ctggcagctg gttggtgctt acaccttggc cgcagcggca ggtccttcca
      121 cgtgctttcg gcggcgacat ggagctggag gagttgggga tccgagagga atgtggcgtg
      181 ttcgggtgca tcgcctcagg agagtggccc acgcagctgg atgtaccgca tgtgatcact
      241 ctgggactcg tggggctgca gcaccggggt caggagagtg ctggtattgt gactagtgat
      301 gggagttcgg tgccaacatt caaatcacac aagggaatgg gtcttgtaaa tcacgtcttt
      361 actgaagaca atttgaaaaa attatatgtt tcaaatcttg gaattggaca caccaggtat
      421 gccaccacag gaaaatgtga actagaaaat tgtcagccct tcgttgttga aacacttcat
      481 gggaagatag ctgtggcaca taatggcgaa ttggtaaatg ctgctcgatt aaggaaaaag
      541 cttctgcgtc atggtattgg tctgtctaca agttctgata gtgaaatgat tacccagtta
      601 ctggcgtata cccctcctca ggaacaagat gacaccccag actgggtagc caggattaaa
      661 aacttgatga aggaagcacc cacagcatac tccctgctta taatgcacag agatgttatt
      721 tatgcagtac gagatcctta tggaaatcgt cccttatgca ttggtcgtct tattccagtg
      781 tctgatataa atgacaaaga gaaaaaaaca tcagaaacag aaggatgggt ggtgtcttca
      841 gaatcttgta gcttcttatc tattggtgca agatattacc gtgaagtctt gcctggagaa
      901 attgtggaaa tatccagaca caatgtccaa actcttgata ttatatcaag gtctgaagga
      961 aacccagtgg ctttttgtat ctttgaatat gtttattttg caagaccaga cagtatgttc
     1021 gaagaccaaa tggtttatac agtaagatac cgttgtggcc agcagctagc gattgaagca
     1081 cctgtggatg cagatttggt tagcactgtt ccagaatctg ctacgcctgc tgctcttgct
     1141 tacgcaggaa agtgtggact tccatatgtg gaggtgctgt gtaaaaaccg gtatgtaggg
     1201 agaaccttca ttcagccaaa catgaggtta agacaacttg gtgttgcaaa aaaatttgga
     1261 gtattgtcag acaactttaa aggcaaaaga attgttcttg tagatgattc aattgtcaga
     1321 ggcaatacca tctcacctat aataaaactg ctcaaagaat ctggtgcaaa agaggtacac
     1381 attcgagtag cttcaccacc aattaaatat ccatgcttca tgggaataaa cattcctaca
     1441 aaagaagagc tcattgccaa taaaccagaa tttgatcacc ttgcagaata tctaggagca
     1501 aacagtgttg tgtatctgtc agtagaagga ctggtttcat ctgtacaaga agggataaag
     1561 tttaaaaaac agaaagagaa aaagcacgat attatgatcc aagaaaatgg aaatggtctg
     1621 gaatgttttg aaaagagtgg tcattgtaca gcttgtctca ctggaaaata tcctgtagaa
     1681 ttagaatggt agctggtagg gttggatgtg tgtagtttca agatagaaag ttggtcaaga
     1741 agttatagtg gtcacacctc atctatttac tgttactcag ttggtacaat gtaaaatgcc
     1801 atgcttatgt ttataagttt tgagattttt tttttttctg aaaaggatac caaagtgcga
     1861 taactgaaca tttccaattg catataatac aacaatatgt ggtgttcttt tttttacaca
     1921 agcattggct agccttttta acctggtcag agaaggcagg tggtcactga catttcccaa
     1981 gtccatgctt taaagggttt gcaagaagtt agggttaagg agaggtgatg ccaacaagac
     2041 aggtgagtta aatataccat ttcacacaaa gtttgaatag aatacattat acctcatagg
     2101 tgtctagcct ctacagttct ggctgtagtt atgaccttgg cttccctgtc taactgtaga
     2161 caaatcttta aaaaaaaata ataataatct ggtgcctcag tttccccaca tgtgcaatgg
     2221 gatacttatt aaataattaa taagaatgtg aataagtgtc atacttttgt gatttgagcc
     2281 atcatttcac ttctgatttt aagacaactc atgattgtta gctttcagaa agctaatgat
     2341 tgttaacttt ttgaaattag tttacaatta attaagattt cattatgatg gaaggagaca
     2401 taattggcag atctttgcca tctctctttg agatgtccta aaaagggttg taaaaatctg
     2461 tgaaaaagtt tttcctacat ttgactagaa aatgtgatcc atagtattta gtgccctgat
     2521 actataagct cagcaagtaa cctggtacat ttgaaataaa aaccaaattt ttagattcaa
     2581 acaatccctt tatccttaat ttaattaatt atcatatgct ttttttaatg aagtgcttga
     2641 tcacttgcaa acatatatac atgtagatgt acatatacat gtacacatac acataaatat
     2701 tattgcaatt aagtgatcaa gtacagacac aataggggcc agttttgttt aaggatcaaa
     2761 gagacaacca ctttggggaa ttagtatcaa cttacaatcc aagtccaagt atcatcttat
     2821 aatcactttt ttctactata ttaagatcta atgaatttga tttctttttt gaagtttttt
     2881 cttgtaacat ctgagattag aagtttaaga tgacttgacc ccaaaccttt gtttatgtaa
     2941 gaatttttaa acataaaagt gtttgtttct gttatgttac cataatttga tgtatatagt
     3001 gtccagatcc atttagaaat ttaatattta ttaataactg aaactgtttg tcttcctttg
     3061 gtatatagtc tcgcatatta tattatagca ggccaagata aaattttgac agctctttaa
     3121 gcccacatgc agcagtgggt cagataaccc tgtggcagtg acacgggcaa attggcattt
     3181 gaataaagcc ctgggaccac ctcaacatgc gtagcctctt gtcttaaatg tactccccat
     3241 ggcagcatgg aggaggcaag acctgtgggt caattttgaa ctggccttac tttgattttt
     3301 aaaacaagag actcagggaa agtactaaac caaaatctct gattttactt tgcgttttct
     3361 gtagtttttg ttttactgag atgcttttgt aaaggaaaat aatactgtga cagtttagta
     3421 attctacaga ttcttaatat ttctccatca tggcctttta cttcacaatt ttctgaagtc
     3481 tgaattcaat tacaattttt ttttaccaat ttaatctcaa atgttgttta actgctttaa
     3541 attcatatac gtagagtatt ataaactgca gagatgaaaa atgtgttttc acgggattta
     3601 tattgtgaac taaactaagc ctactttttg tgacttattt gtgatgcctt gttgataaat
     3661 atgtgtaata agtatgttta aa
//
