LOCUS       NM_021129               1292 bp    mRNA    linear   PRI 24-APR-2024
DEFINITION  Homo sapiens inorganic pyrophosphatase 1 (PPA1), mRNA.
ACCESSION   NM_021129
VERSION     NM_021129.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Zheng,S., Zheng,C., Chen,S., Guo,J., Huang,L., Huang,Z., Xu,S.,
            Wu,Y., Li,S., Lin,J., You,Y. and Hu,F.
  TITLE     Structural and biochemical characterization of active sites mutant
            in human inorganic pyrophosphatase
  JOURNAL   Biochim Biophys Acta Gen Subj 1868 (5), 130594 (2024)
   PUBMED   38428647
  REMARK    GeneRIF: Structural and biochemical characterization of active
            sites mutant in human inorganic pyrophosphatase.
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Kirkbride,J.A., Nilsson,G.Y., Kim,J.I., Takeya,K., Tanaka,Y.,
            Tokumitsu,H., Suizu,F. and Eto,M.
  TITLE     PHI-1, an Endogenous Inhibitor Protein for Protein Phosphatase-1
            and a Pan-Cancer Marker, Regulates Raf-1 Proteostasis
  JOURNAL   Biomolecules 13 (12), 1741 (2023)
   PUBMED   38136612
  REMARK    GeneRIF: PHI-1, an Endogenous Inhibitor Protein for Protein
            Phosphatase-1 and a Pan-Cancer Marker, Regulates Raf-1
            Proteostasis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1292)
  AUTHORS   Niu,T., Zhu,J., Dong,L., Yuan,P., Zhang,L. and Liu,D.
  TITLE     Inorganic pyrophosphatase 1 activates the phosphatidylinositol
            3-kinase/Akt signaling to promote tumorigenicity and stemness
            properties in colorectal cancer
  JOURNAL   Cell Signal 108, 110693 (2023)
   PUBMED   37141926
  REMARK    GeneRIF: Inorganic pyrophosphatase 1 activates the
            phosphatidylinositol 3-kinase/Akt signaling to promote
            tumorigenicity and stemness properties in colorectal cancer.
REFERENCE   4  (bases 1 to 1292)
  AUTHORS   Troyanovsky,R.B., Indra,I., Kato,R., Mitchell,B.J. and
            Troyanovsky,S.M.
  TITLE     Basolateral protein Scribble binds phosphatase PP1 to establish a
            signaling network maintaining apicobasal polarity
  JOURNAL   J Biol Chem 297 (5), 101289 (2021)
   PUBMED   34634305
  REMARK    GeneRIF: Basolateral protein Scribble binds phosphatase PP1 to
            establish a signaling network maintaining apicobasal polarity.
REFERENCE   5  (bases 1 to 1292)
  AUTHORS   Saidy,B., Kotecha,S., Butler,A., Rakha,E.A., Ellis,I.O.,
            Green,A.R., Martin,S.G. and Storr,S.J.
  TITLE     PP1, PKA and DARPP-32 in breast cancer: A retrospective assessment
            of protein and mRNA expression
  JOURNAL   J Cell Mol Med 25 (11), 5015-5024 (2021)
   PUBMED   33991172
  REMARK    GeneRIF: PP1, PKA and DARPP-32 in breast cancer: A retrospective
            assessment of protein and mRNA expression.
REFERENCE   6  (bases 1 to 1292)
  AUTHORS   Fukami,M.H., Dangelmaier,C.A., Bauer,J.S. and Holmsen,H.
  TITLE     Secretion, subcellular localization and metabolic status of
            inorganic pyrophosphate in human platelets. A major constituent of
            the amine-storing granules
  JOURNAL   Biochem J 192 (1), 99-105 (1980)
   PUBMED   6118133
REFERENCE   7  (bases 1 to 1292)
  AUTHORS   Thuillier,L.
  TITLE     Purification and kinetic properties of human erythrocyte
            Mg2+-dependent inorganic pyrophosphatase
  JOURNAL   Biochim Biophys Acta 524 (1), 198-206 (1978)
   PUBMED   656444
REFERENCE   8  (bases 1 to 1292)
  AUTHORS   McAlpine,P.J., Mohandas,T., Ray,M., Wang,H. and Hamerton,J.L.
  TITLE     Assignment of the inorganic pyrophosphatase gene locus (PP) to
            chromosome 10 in man
  JOURNAL   Cytogenet Cell Genet 16 (1-5), 201-203 (1976)
   PUBMED   975879
REFERENCE   9  (bases 1 to 1292)
  AUTHORS   Fisher,R.A., Turner,B.M., Dorkin,H.L. and Harris,H.
  TITLE     Studies on human erythrocyte inorganic pyrophosphatase
  JOURNAL   Ann Hum Genet 37 (3), 341-353 (1974)
   PUBMED   4130389
REFERENCE   10 (bases 1 to 1292)
  AUTHORS   Pynes,G.D. and Younathan,E.S.
  TITLE     Purification and some properties of inorganic pyrophosphatase from
            human erythrocytes
  JOURNAL   J Biol Chem 242 (9), 2119-2123 (1967)
   PUBMED   6022858
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL731540.4 and AB026723.1.
            
            On Nov 22, 2018 this sequence version replaced NM_021129.3.
            
            Summary: The protein encoded by this gene is a member of the
            inorganic pyrophosphatase (PPase) family. PPases catalyze the
            hydrolysis of pyrophosphate to inorganic phosphate, which is
            important for the phosphate metabolism of cells. Studies of a
            similar protein in bovine suggested a cytoplasmic localization of
            this enzyme. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.440882.1,
                                           SRR3476690.930360.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373232.8/ ENSP00000362329.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-166               AL731540.4         175806-175971       c
            167-225             AL731540.4         172883-172941       c
            226-279             AL731540.4         161306-161359       c
            280-399             AL731540.4         160354-160473       c
            400-486             AL731540.4         157042-157128       c
            487-613             AL731540.4         156005-156131       c
            614-741             AL731540.4         152100-152227       c
            742-827             AL731540.4         151747-151832       c
            828-897             AL731540.4         148806-148875       c
            898-940             AL731540.4         147415-147457       c
            941-1254            AL731540.4         145415-145728       c
            1255-1292           AB026723.1         1183-1220
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q22.1"
     gene            1..1292
                     /gene="PPA1"
                     /gene_synonym="HEL-S-66p; IOPPP; PP; PP1; SID6-8061"
                     /note="inorganic pyrophosphatase 1"
                     /db_xref="GeneID:5464"
                     /db_xref="HGNC:HGNC:9226"
                     /db_xref="MIM:179030"
     exon            1..166
                     /gene="PPA1"
                     /gene_synonym="HEL-S-66p; IOPPP; PP; PP1; SID6-8061"
                     /inference="alignment:Splign:2.1.0"
     CDS             103..972
                     /gene="PPA1"
                     /gene_synonym="HEL-S-66p; IOPPP; PP; PP1; SID6-8061"
                     /EC_number="3.6.1.1"
                     /note="pyrophosphate phospho-hydrolase; pyrophosphatase
                     (inorganic) 1; cytosolic inorganic pyrophosphatase;
                     diphosphate phosphohydrolase; PPase; pyrophosphatase 1;
                     epididymis secretory sperm binding protein Li 66p;
                     inorganic diphosphatase 1"
                     /codon_start=1
                     /product="inorganic pyrophosphatase"
                     /protein_id="NP_066952.1"
                     /db_xref="CCDS:CCDS7299.1"
                     /db_xref="GeneID:5464"
                     /db_xref="HGNC:HGNC:9226"
                     /db_xref="MIM:179030"
                     /translation="MSGFSTEERAAPFSLEYRVFLKNEKGQYISPFHDIPIYADKDVF
                     HMVVEVPRWSNAKMEIATKDPLNPIKQDVKKGKLRYVANLFPYKGYIWNYGAIPQTWE
                     DPGHNDKHTGCCGDNDPIDVCEIGSKVCARGEIIGVKVLGILAMIDEGETDWKVIAIN
                     VDDPDAANYNDINDVKRLKPGYLEATVDWFRRYKVPDGKPENEFAFNAEFKDKDFAID
                     IIKSTHDHWKALVTKKTNGKGISCMNTTLSESPFKCDPDAARAIVDALPPPCESACTV
                     PTDVDKWFHHQKN"
     exon            167..225
                     /gene="PPA1"
                     /gene_synonym="HEL-S-66p; IOPPP; PP; PP1; SID6-8061"
                     /inference="alignment:Splign:2.1.0"
     exon            226..279
                     /gene="PPA1"
                     /gene_synonym="HEL-S-66p; IOPPP; PP; PP1; SID6-8061"
                     /inference="alignment:Splign:2.1.0"
     exon            280..399
                     /gene="PPA1"
                     /gene_synonym="HEL-S-66p; IOPPP; PP; PP1; SID6-8061"
                     /inference="alignment:Splign:2.1.0"
     exon            400..486
                     /gene="PPA1"
                     /gene_synonym="HEL-S-66p; IOPPP; PP; PP1; SID6-8061"
                     /inference="alignment:Splign:2.1.0"
     exon            487..613
                     /gene="PPA1"
                     /gene_synonym="HEL-S-66p; IOPPP; PP; PP1; SID6-8061"
                     /inference="alignment:Splign:2.1.0"
     exon            614..741
                     /gene="PPA1"
                     /gene_synonym="HEL-S-66p; IOPPP; PP; PP1; SID6-8061"
                     /inference="alignment:Splign:2.1.0"
     exon            742..827
                     /gene="PPA1"
                     /gene_synonym="HEL-S-66p; IOPPP; PP; PP1; SID6-8061"
                     /inference="alignment:Splign:2.1.0"
     exon            828..897
                     /gene="PPA1"
                     /gene_synonym="HEL-S-66p; IOPPP; PP; PP1; SID6-8061"
                     /inference="alignment:Splign:2.1.0"
     exon            898..940
                     /gene="PPA1"
                     /gene_synonym="HEL-S-66p; IOPPP; PP; PP1; SID6-8061"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1292
                     /gene="PPA1"
                     /gene_synonym="HEL-S-66p; IOPPP; PP; PP1; SID6-8061"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1272..1277
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PPA1"
                     /gene_synonym="HEL-S-66p; IOPPP; PP; PP1; SID6-8061"
                     /note="hexamer: AATAAA"
     polyA_site      1292
                     /gene="PPA1"
                     /gene_synonym="HEL-S-66p; IOPPP; PP; PP1; SID6-8061"
                     /note="major polyA site"
ORIGIN      
        1 ccagtgctcg cagtgcgcag gcgtggggct ctctccttgt cagtcggcgc cgcgtgcggg
       61 ctggtggctc tgtggcagcg gcggcggcag gactccggca ctatgagcgg cttcagcacc
      121 gaggagcgcg ccgcgccctt ctccctggag taccgagtct tcctcaaaaa tgagaaagga
      181 caatatatat ctccatttca tgatattcca atttatgcag ataaggatgt gtttcacatg
      241 gtagttgaag taccacgctg gtctaatgca aaaatggaga ttgctacaaa ggacccttta
      301 aaccctatta aacaagatgt gaaaaaagga aaacttcgct atgttgcgaa tttgttcccg
      361 tataaaggat atatctggaa ctatggtgcc atccctcaga cttgggaaga cccagggcac
      421 aatgataaac atactggctg ttgtggtgac aatgacccaa ttgatgtgtg tgaaattgga
      481 agcaaggtat gtgcaagagg tgaaataatt ggcgtgaaag ttctaggcat attggctatg
      541 attgacgaag gggaaaccga ctggaaagtc attgccatta atgtggatga tcctgatgca
      601 gccaattata atgatatcaa tgatgtcaaa cggctgaaac ctggctactt agaagctact
      661 gtggactggt ttagaaggta taaggttcct gatggaaaac cagaaaatga gtttgcgttt
      721 aatgcagaat ttaaagataa ggactttgcc attgatatta ttaaaagcac tcatgaccat
      781 tggaaagcat tagtgactaa gaaaacgaat ggaaaaggaa tcagttgcat gaatacaact
      841 ttgtctgaga gccccttcaa gtgtgatcct gatgctgcca gagccattgt ggatgcttta
      901 ccaccaccct gtgaatctgc ctgcacagta ccaacagacg tggataagtg gttccatcac
      961 cagaaaaact aatgagattt ctctggaata caagctgata ttgctacatc gtgttcatct
     1021 ggatgtatta gaagtaaaag tagtagcttt tcaaagcttt aaatttgtag aactcatcta
     1081 actaaagtaa attctgctgt gactaatcca atatactcag aatgttatcc atctaaagca
     1141 tttttcatat ctcaactaag ataactttta gcacatgctt aaatatcaaa gcagttgtca
     1201 tttggaagtc acttgtgaat agatgtgcaa ggggagcaca tattggatgt atatgttacc
     1261 atatgttagg aaataaaatt attttgctga aa
//
