LOCUS       NM_001370959            6123 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens POU class 6 homeobox 2 (POU6F2), transcript variant 3,
            mRNA.
ACCESSION   NM_001370959 XM_011515113
VERSION     NM_001370959.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Cho,H.J., Gurbuz,F., Stamou,M., Kotan,L.D., Farmer,S.M., Can,S.,
            Tompkins,M.F., Mammadova,J., Altincik,S.A., Gokce,C., Catli,G.,
            Bugrul,F., Bartlett,K., Turan,I., Balasubramanian,R., Yuksel,B.,
            Seminara,S.B., Wray,S. and Topaloglu,A.K.
  TITLE     POU6F2 mutation in humans with pubertal failure alters GnRH
            transcript expression
  JOURNAL   Front Endocrinol (Lausanne) 14, 1203542 (2023)
   PUBMED   37600690
  REMARK    GeneRIF: POU6F2 mutation in humans with pubertal failure alters
            GnRH transcript expression.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 6123)
  AUTHORS   Miao,Y., Li,C., Guo,J., Wang,H., Gong,L., Xie,W. and Zhang,Y.
  TITLE     Identification of a novel somatic mutation of POU6F2 by
            whole-genome sequencing in prolactinoma
  JOURNAL   Mol Genet Genomic Med 7 (12), e1022 (2019)
   PUBMED   31692290
  REMARK    GeneRIF: Whole-genome sequencing identified two novel somatic
            mutations in the POU6F2 gene (c. 839 C>T; c. 875A>G) in patients
            with prolactinoma. The mutations obviously decreased the expression
            level of POU6F2. Inhibition its activity increased the cell
            proliferation and PRL secretion in pituitary tumor cell line, but
            proliferation and PRL secretion were decreased in cells with POU6F2
            overexpression.
REFERENCE   4  (bases 1 to 6123)
  AUTHORS   King,R., Struebing,F.L., Li,Y., Wang,J., Koch,A.A., Cooke
            Bailey,J.N., Gharahkhani,P., MacGregor,S., Allingham,R.R.,
            Hauser,M.A., Wiggs,J.L. and Geisert,E.E.
  CONSRTM   International Glaucoma Genetics Consortium; NEIGHBORHOOD Consortium
  TITLE     Genomic locus modulating corneal thickness in the mouse identifies
            POU6F2 as a potential risk of developing glaucoma
  JOURNAL   PLoS Genet 14 (1), e1007145 (2018)
   PUBMED   29370175
  REMARK    GeneRIF: Genomic loci were examined in the NEIGHBORHOOD database to
            determine if they are potential risk factors for human glaucoma
            identified using meta-data from human GWAS. The top 50 hits all
            resided within one gene (POU6F2), with the highest significance
            level of p = 10-6 for SNP rs76319873. POU6F2 is found in retinal
            ganglion cells and in corneal limbal stem cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6123)
  AUTHORS   Yoshihara,M., Hara,S., Tsujikawa,M., Kawasaki,S., Hayashizaki,Y.,
            Itoh,M., Kawaji,H. and Nishida,K.
  TITLE     Restricted Presence of POU6F2 in Human Corneal Endothelial Cells
            Uncovered by Extension of the Promoter-level Expression Atlas
  JOURNAL   EBioMedicine 25, 175-186 (2017)
   PUBMED   29113774
  REMARK    GeneRIF: We further discovered that the expression of transcription
            factor POU class 6 homeobox 2 (POU6F2) is restricted to Corneal
            endothelial cells (CECs) , and upregulated during human CEC
            differentiation, suggesting that POU6F2 is pivotal to terminal
            differentiation of CECs
REFERENCE   6  (bases 1 to 6123)
  AUTHORS   Perotti,D., Vecchi,G.D., Lualdi,E., Testi,M.A., Sozzi,G.,
            Collini,P., Spreafico,F., Terenziani,M., Fossati-Bellani,F. and
            Radice,P.
  TITLE     Wilms tumor in monozygous twins: clinical, pathological,
            cytogenetic and molecular case report
  JOURNAL   J Pediatr Hematol Oncol 27 (10), 521-525 (2005)
   PUBMED   16217254
REFERENCE   7  (bases 1 to 6123)
  AUTHORS   Perotti,D., De Vecchi,G., Testi,M.A., Lualdi,E., Modena,P.,
            Mondini,P., Ravagnani,F., Collini,P., Di Renzo,F., Spreafico,F.,
            Terenziani,M., Sozzi,G., Fossati-Bellani,F. and Radice,P.
  TITLE     Germline mutations of the POU6F2 gene in Wilms tumors with loss of
            heterozygosity on chromosome 7p14
  JOURNAL   Hum Mutat 24 (5), 400-407 (2004)
   PUBMED   15459955
  REMARK    GeneRIF: observations suggest that POU6F2 is a tumor suppressor and
            is involved in hereditary predisposition to Wilms tumor
REFERENCE   8  (bases 1 to 6123)
  AUTHORS   Perotti,D., Testi,M.A., Mondini,P., Pilotti,S., Green,E.D.,
            Pession,A., Sozzi,G., Pierotti,M.A., Fossati-Bellani,F. and
            Radice,P.
  TITLE     Refinement within single yeast artificial chromosome clones of a
            minimal region commonly deleted on the short arm of chromosome 7 in
            Wilms tumours
  JOURNAL   Genes Chromosomes Cancer 31 (1), 42-47 (2001)
   PUBMED   11284034
REFERENCE   9  (bases 1 to 6123)
  AUTHORS   Phillips,K. and Luisi,B.
  TITLE     The virtuoso of versatility: POU proteins that flex to fit
  JOURNAL   J Mol Biol 302 (5), 1023-1039 (2000)
   PUBMED   11183772
  REMARK    Review article
REFERENCE   10 (bases 1 to 6123)
  AUTHORS   Zhou,H., Yoshioka,T. and Nathans,J.
  TITLE     Retina-derived POU-domain factor-1: a complex POU-domain gene
            implicated in the development of retinal ganglion and amacrine
            cells
  JOURNAL   J Neurosci 16 (7), 2261-2274 (1996)
   PUBMED   8601806
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011292.3, AC073345.11,
            AC092174.5 and AC005483.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 17, 2019 this sequence version replaced XM_011515113.2.
            
            Summary: This gene encodes a member of the POU protein family
            characterized by the presence of a bipartite DNA binding domain,
            consisting of a POU-specific domain and a homeodomain, separated by
            a variable polylinker. The DNA binding domain may bind to DNA as
            monomers or as homo- and/or heterodimers, in a sequence-specific
            manner. The POU family members are transcriptional regulators, many
            of which are known to control cell type-specific differentiation
            pathways. This gene is a tumor suppressor involved in Wilms tumor
            (WT) predisposition. Alternatively spliced transcript variants
            encoding distinct isoforms have been found for this gene.[provided
            by RefSeq, Oct 2009].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1968968, SAMEA2142348
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000518318.7/ ENSP00000430514.3
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-150               AC011292.3         55361-55510
            151-322             AC073345.11        56375-56546
            323-414             AC092174.5         31915-32006
            415-643             AC092174.5         35072-35300
            644-1017            AC005483.1         150160-150533       c
            1018-1158           AC005483.1         83435-83575         c
            1159-1365           AC005483.1         56892-57098         c
            1366-1534           AC005483.1         38474-38642         c
            1535-1703           AC005483.1         29460-29628         c
            1704-6123           AC005483.1         21574-25993         c
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p14.1"
     gene            1..6123
                     /gene="POU6F2"
                     /gene_synonym="RPF-1; WT5; WTSL"
                     /note="POU class 6 homeobox 2"
                     /db_xref="GeneID:11281"
                     /db_xref="HGNC:HGNC:21694"
                     /db_xref="MIM:609062"
     exon            1..150
                     /gene="POU6F2"
                     /gene_synonym="RPF-1; WT5; WTSL"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..2208
                     /gene="POU6F2"
                     /gene_synonym="RPF-1; WT5; WTSL"
                     /note="isoform 3 is encoded by transcript variant 3; Wilms
                     tumor suppressor locus; retina-derived POU-domain
                     factor-1; POU domain, class 6, transcription factor 2"
                     /codon_start=1
                     /product="POU domain, class 6, transcription factor 2
                     isoform 3"
                     /protein_id="NP_001357888.1"
                     /db_xref="CCDS:CCDS94088.1"
                     /db_xref="GeneID:11281"
                     /db_xref="HGNC:HGNC:21694"
                     /db_xref="MIM:609062"
                     /translation="MHARNPHSPFQRQHMMDCYLSAQQDTGTMQAVIGQDPMIAGQVS
                     KPLLSVRSEMNAELRGEDKAATSDSELNEPLLAPVESNDSEDTPSKLFGARGNPALSD
                     PGTPDQHQASQTHPPFPVGPQPLLTAQQLASAVAGVMPGGPPALNQPILIPFNMAGQL
                     GGQQGLVLTLPTANLTNIQGLVAAAAAGGIMTLPLQNLQATSSLNSQLQQLQLQLQQQ
                     QQQQQQQPPPSTNQHPQPAPQAPSQSQQQPLQPTPPQQPPPASQQPPAPTSQLQQAPQ
                     PQQHQPHSHSQNQNQPSPTQQSSSPPQKPSQSPGHGLPSPLTPPNPLQLVNNPLASQA
                     AAAAAAMSSIASSQAFGNALSSLQGVTGQLVTNAQGQIIGTIPLMPNPGPSSQAASGT
                     QGLQVQPITPQLLTNAQGQIIATVIGNQILPVINTQGITLSPIKPGQQLHQPSQTSVG
                     QAASQGNLLHLAHSQASMSQSPVRQASSSSSSSSSSSALSVGQLVSNPQTAAGEVDGV
                     NLEEIREFAKAFKIRRLSLGLTQTQVGQALSATEGPAYSQSAICRHTILRSHFFLPQE
                     AQENTIASSLTAKLNPGLLYPARFEKLDITPKSAQKIKPVLERWMAEAEARHRAGMQN
                     LTEFIGSEPSKKRKRRTSFTPQALEILNAHFEKNTHPSGQEMTEIAEKLNYDREVVRV
                     WFCNKRQALKNTIKRLKQHEPATAVPLEPLTDSLEENS"
     exon            151..322
                     /gene="POU6F2"
                     /gene_synonym="RPF-1; WT5; WTSL"
                     /inference="alignment:Splign:2.1.0"
     exon            323..414
                     /gene="POU6F2"
                     /gene_synonym="RPF-1; WT5; WTSL"
                     /inference="alignment:Splign:2.1.0"
     exon            415..643
                     /gene="POU6F2"
                     /gene_synonym="RPF-1; WT5; WTSL"
                     /inference="alignment:Splign:2.1.0"
     exon            644..1017
                     /gene="POU6F2"
                     /gene_synonym="RPF-1; WT5; WTSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1158
                     /gene="POU6F2"
                     /gene_synonym="RPF-1; WT5; WTSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1159..1365
                     /gene="POU6F2"
                     /gene_synonym="RPF-1; WT5; WTSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1534
                     /gene="POU6F2"
                     /gene_synonym="RPF-1; WT5; WTSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1703
                     /gene="POU6F2"
                     /gene_synonym="RPF-1; WT5; WTSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..6123
                     /gene="POU6F2"
                     /gene_synonym="RPF-1; WT5; WTSL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6102..6107
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POU6F2"
                     /gene_synonym="RPF-1; WT5; WTSL"
                     /note="hexamer: AATAAA"
     polyA_site      6123
                     /gene="POU6F2"
                     /gene_synonym="RPF-1; WT5; WTSL"
                     /note="major polyA site"
ORIGIN      
        1 cccttgcttt tggtgatggt tgtgagtgca gaagttgatt gacagatgca tgccagaaac
       61 ccccattctc cttttcaaag acaacatatg atggattgct atctctcagc gcagcaggac
      121 acggggacca tgcaagctgt aattggtcag gatccaatga tagctggaca agtcagtaag
      181 cccttgctgt cagtgcggag tgaaatgaat gcggagttga gaggtgagga caaggctgct
      241 acttcagaca gcgagctgaa tgagcccctg cttgcgcctg tggaatcaaa tgacagcgag
      301 gacactccca gcaagctctt cggggctaga ggaaacccag cattatcaga cccaggcact
      361 cctgaccaac accaggccag tcagacccac cccccatttc cagttgggcc acagccactt
      421 ctgacggcac agcagttagc ttctgctgtg gccggcgtga tgccgggagg ccccccagcc
      481 ctcaaccagc caatcctcat tcccttcaac atggcgggac agctaggagg ccagcaagga
      541 ctggttctca cactgccaac agcgaatctc accaacatcc aagggctggt ggcagcagct
      601 gcagccggag gcattatgac tctgccactg caaaatctac aagctacctc atccctgaac
      661 tcccagctcc agcagctcca gctccagctc cagcagcagc agcagcagca gcagcagcag
      721 cctcccccgt caaccaacca gcacccgcaa ccagccccac aggcgccctc gcagtcccag
      781 cagcagccgc tgcagcccac cccaccccag cagccaccac ccgcctctca gcagccgcca
      841 gctcctacat ctcagctgca acaggcgcct cagccccagc agcaccaacc ccactcccac
      901 tcccagaacc agaaccaacc atctccaacc cagcagagct ccagcccccc gcagaaacct
      961 agtcagtctc caggacatgg cctgccttca ccgctcacgc cacccaatcc tctacagctg
     1021 gttaataatc cactagcaag tcaggctgca gcggctgcag cagccatgag ctccatagca
     1081 agctcacagg cctttggcaa tgccctctcc agtcttcagg gggtcacagg tcaactagtt
     1141 actaatgcac aaggacagat tatcgggacc attccactga tgcctaatcc agggccatcg
     1201 agccaagcag caagcggcac tcagggcttg caagtgcagc caatcacccc ccagctcctc
     1261 acaaacgccc agggccagat catcgccaca gtcattggga accagatcct gcccgtgatc
     1321 aacacccagg gcatcacgct gtcacccatc aagcccggcc agcagctcca ccaaccctcc
     1381 cagacgtcag tgggtcaagc agcctcccaa ggcaaccttc tgcacctggc tcacagccaa
     1441 gcatccatgt ctcaaagtcc cgtccggcag gcttcctctt cttcctcctc atcctcctct
     1501 tcttcagctt tgagcgtggg ccagttagtc agcaatcctc aaacggcagc gggtgaggtg
     1561 gatggggtta atctggagga gatccgagaa tttgccaaag cttttaaaat ccggcgcctg
     1621 tcccttggcc tgacccagac tcaggtggga caggctctca gtgctacaga gggccccgcg
     1681 tacagccagt cggccatctg cagacacacc atcctgagaa gccacttttt cctaccacag
     1741 gaagcccaag agaacactat agctagcagt ctgacagcca aactgaaccc tggccttttg
     1801 tatcctgcca ggtttgaaaa gctggacatc acccctaaaa gtgcccagaa gatcaagccg
     1861 gtgcttgagc ggtggatggc tgaggctgag gcccgccatc gagcaggtat gcagaacctg
     1921 accgagttta tcgggagtga accatccaaa aagcgcaagc ggcgcacctc cttcacaccc
     1981 caggcccttg agatcctcaa tgcccacttt gagaagaaca cacacccttc tgggcaggaa
     2041 atgaccgaaa ttgctgagaa gctgaactat gaccgagaag tagttagagt ttggttctgc
     2101 aataagaggc aagccctgaa gaacacaatt aaacgcttaa aacagcacga gccggccacg
     2161 gcagtccctt tggagccctt aacagactct ctggaagaaa actcctaaag agatgcccac
     2221 ccataatcag aagcaaaatt cacagaaact aaactccacc cttgggactc cacaacaaca
     2281 acaacaacaa aatttaattt aatttaaaaa tagccccagt cgtcatcacc cttgtaagta
     2341 aatgactaag aaaactacca agtggacaga atggtttcta catgtccgtt ggttttccaa
     2401 aaaggaaaga agaaaatttt tagaaaattt ttaaacaagg aatacaccac actgaaggtg
     2461 tgtgtggtag gatagttccc ttcccccacc tgtctccccc aaagccagtt ttttaatgga
     2521 cttaaagcaa accaaataac cacgtacttt tttctgtata ttatgaaaat gtgaacacat
     2581 tttaaggaaa aagaaaaaaa aaaactaaac caaaaaccaa caacgaagga acaaaaactt
     2641 tgatctgttc aaagcgaata caagcctgcc acctggagga aggactgcac cccttcaggt
     2701 actaagtgct gattcactat gaaacctatt aaccaaagtc agaaacatgg cattgcaacg
     2761 cgatcgttcg tctacgcttc tccgcacgta aagttgtgtt acgaattttt acatttgtac
     2821 taacagaaca ataggaagcc tgatttctcc catctttccc atctactgtc tccacccatg
     2881 gggtgggtac cattgttgaa gccattctgt gaggctcact attgggtttt tttgtggggg
     2941 tggtagggag ggtggtcttt tttctttgtc tttctttttg acgggagggc atcctggatc
     3001 gtgtgccaaa gcatttgttg cttttttctc actatgactt gtgggtttga gaaaagaaaa
     3061 tggagctcgc atttctctct tttcctccat ttctccatct ccctcgcgcg tggctcctgg
     3121 ggtctgctgg aaggccacag aagtggggag aagcagtgtc ttttccactc agatcctagt
     3181 gaaatgcagg agagactcca aaataactag ggctttgctc gatgaactgt caacactggc
     3241 ataagctgta attgtgctca ctgtccacac cagagcttgg gatttttctc agtctgttgg
     3301 ccacgtacat ggagagctga ccaaaactaa ttttgtaata taaacataag ctgcacattt
     3361 ggttcaatac ttacatctat gttatgcttc tgtgcaaagc aatttctctc agaagtctga
     3421 tagccaaaga aatgtctcaa gatttcagtc aaatacacac atgacatgca cacacccata
     3481 aacacacaca caaagaaaca ggctaaaaag aaagtgatag caactggatc attaaaggcc
     3541 atcgtgtatc ctgttaatct catcttttct gctacttctt tcattccaat agcgatggca
     3601 gacttttttc agggggaata attactcttc gggatatata aatggagcag aaaggtggcg
     3661 ggcaaattta aacaactgga attgggggcc aggttcttca ggggaaagga agtttgaaga
     3721 agctttcacc aaaagaaaaa aatatagaag gggcttatct aacaatcaga atagcctgca
     3781 atatgagaac tagaggatta tttcctttaa aaaaaataac ttaaaagatc tgtgggccac
     3841 aataacctaa taagcaagga catgttggga tgtgaggacg gctgtgcagg tgagcacttg
     3901 gcagctgcca ctggtgtttt cacccaggta caggcagacg gtgttccatt aaaggagcag
     3961 gcaaaagaga gctgtcaatt tgcttgctgt tgaaatgtac atatttatgc ataatttata
     4021 atcatgctaa tgtattatct agaagtaagt tgtgaaagaa aaaagagaaa cccaatctca
     4081 agttgcccaa ctgattagaa atgccatctc cttcccctaa tacactccct ctccctgggg
     4141 agtttgagtt tcttctgttg gctcctttgc tgtttgtgta aatgctacag tataattgtc
     4201 ccctccaatg attcatgcag caatcaagag actaccagca aggaaactct aaaaggaatc
     4261 ctggaagtgg aacaagtcac tcgtaaaggt gtttccattt taccagtcaa tgctaactgg
     4321 accacagcta acttgatctt caatgccaac atcacagagc ccctttcgcc acctcaaaaa
     4381 ctgcacaaac atggacaacc cagaaaccca aaactttatt gattaaaaca gattttggat
     4441 tctcctagga cctgctccaa attccatcaa gaaaagctcc ctgcgaagag aagttaacaa
     4501 gataacatat gatggatgct aaatatttaa atatatgccg gcagcggtta cataaggcct
     4561 gctcagttct gagatctata attgggaggg tctaactagg acagggaaaa tataaaataa
     4621 aagtaatctt ccagagtgga acaaaaaaga aatatattca tccgacagtc gtaacaccat
     4681 tcatgtgcag tgattttttt atagttttat aattttgtat tgttttataa attatttata
     4741 aggtgttgta atgcttctta tattaatttt ttacagataa attttttgct acaaggcata
     4801 aaaaggtgcc tgaaccgatt cttaggaata taaattatac tgtgtaactc ataagtcttt
     4861 gggaccacac ctaatgctta attttatgat gacatttctc atgtctactt gtaaacaact
     4921 tcatatgcca atggcgttta agtgtctttt atgtttccat gaactgaagg ttaaggttgc
     4981 cacccacaaa aaataaaagc cactttgggc atatgtgatt gtatttcaag cttcagtatt
     5041 tttctcacat aatttttaat tattgttatc tgcattttca ttacttcatg aaaaaatgac
     5101 agtggatatt acattttaca cttgtttata gattttcttt ctttttcaac caagaaaata
     5161 ttttgaacaa ttgaaacttt taattgcatt tgcaaggttt tggcttcttt atgttgtcat
     5221 cacacagatg cacacacaca tacacataca acacaaaaga gagaacaaaa aagcaaaagg
     5281 agctaggaaa aaaacaaaac aaaacaagta gaggtgtatc aaagaactca agctataacc
     5341 aaaaagaaat cgtaaaatgc ctttgctcgt ttctctacgc tggaccaaag ctcaatattt
     5401 gtaggtatat gcacattgta tagatatggc taaatgttgc tgacaatctc gcaatactaa
     5461 actgttccta ttttaagaaa aaaaaaagaa atacaaactg ttcatcaatg ttttacctca
     5521 gcactctact tgtacccagt taatgaccaa gcttaaaaaa aaattgagat aaaggaaatt
     5581 gattttcatt tcaatgtttg actgtaaaat ctgtttggat aacattttgt aatgagcttt
     5641 ttgtcatgtg atttgcttgt cttcaacttg aaattatgtg aggcacattt gtttatttgt
     5701 tgttaagaaa gtgatttttt tttttttttg tcctatgtgc tgtgatctag actggtcacc
     5761 ggggtcactt atgaggcacc acaaagagat ctgcttctcc gtgcccggag caggcagcag
     5821 gagggagggc aggaaacagg actgggttgc tttggaaaaa tcatcatgac aagaagttga
     5881 catgatagac ttgtgaccaa gaagccaaac tgaatattta aaagctcctt acttgctctg
     5941 acattgaaac caaagctgat tttatctgca caggttgctt aatatttaaa aaaaaaaaaa
     6001 actgtactta atctagagca atatctgtat ggtcagtaaa gctgcacttt gtgtatttct
     6061 taacagcttc agatctgtca cttttaattt gtaccataaa aaataaataa ttgtttgaca
     6121 tga
//
