LOCUS       NM_002697              13843 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens POU class 2 homeobox 1 (POU2F1), transcript variant 1,
            mRNA.
ACCESSION   NM_002697
VERSION     NM_002697.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13843)
  AUTHORS   Nazarov,I.B., Zilov,D.S., Gordeev,M.N., Potapenko,E.V.,
            Yeremenko,N. and Tomilin,A.N.
  TITLE     Transcriptional Coactivator BOB1 (OBF1, OCA-B) Modulates the
            Specificity of DNA Recognition by the POU-Domain Factors OCT1 and
            OCT2 in a Monomeric Configuration
  JOURNAL   Biomolecules 14 (1), 123 (2024)
   PUBMED   38254723
  REMARK    GeneRIF: Transcriptional Coactivator BOB1 (OBF1, OCA-B) Modulates
            the Specificity of DNA Recognition by the POU-Domain Factors OCT1
            and OCT2 in a Monomeric Configuration.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13843)
  AUTHORS   Kamhieh-Milz,J., Chen,L., Goettsch,C., Pfefferkorn,A.M.,
            Hofmann,A., Brunssen,C., Muller,G.M., Walther,T., Ashraf,M.I.,
            Moll,G., Morawietz,H., Witowski,J. and Catar,R.
  TITLE     Ang II Promotes ET-1 Production by Regulating NOX2 Activity Through
            Transcription Factor Oct-1
  JOURNAL   Arterioscler Thromb Vasc Biol 43 (8), 1429-1440 (2023)
   PUBMED   37381986
  REMARK    GeneRIF: Ang II Promotes ET-1 Production by Regulating NOX2
            Activity Through Transcription Factor Oct-1.
REFERENCE   3  (bases 1 to 13843)
  AUTHORS   Takasawa,S., Shobatake,R., Itaya-Hironaka,A., Makino,M.,
            Uchiyama,T., Sakuramoto-Tsuchida,S., Takeda,Y., Ota,H. and
            Yamauchi,A.
  TITLE     Upregulation of IL-8, osteonectin, and myonectin mRNAs by
            intermittent hypoxia via OCT1- and NRF2-mediated mechanisms in
            skeletal muscle cells
  JOURNAL   J Cell Mol Med 26 (24), 6019-6031 (2022)
   PUBMED   36457269
  REMARK    GeneRIF: Upregulation of IL-8, osteonectin, and myonectin mRNAs by
            intermittent hypoxia via OCT1- and NRF2-mediated mechanisms in
            skeletal muscle cells.
REFERENCE   4  (bases 1 to 13843)
  AUTHORS   Wang,Y., Tan,K., Hu,W., Hou,Y. and Yang,G.
  TITLE     LncRNA AC026401.3 interacts with OCT1 to intensify sorafenib and
            lenvatinib resistance by activating E2F2 signaling in
            hepatocellular carcinoma
  JOURNAL   Exp Cell Res 420 (1), 113335 (2022)
   PUBMED   36084669
  REMARK    GeneRIF: LncRNA AC026401.3 interacts with OCT1 to intensify
            sorafenib and lenvatinib resistance by activating E2F2 signaling in
            hepatocellular carcinoma.
REFERENCE   5  (bases 1 to 13843)
  AUTHORS   Doetsch,D.A., Ansari,S., Jensen,O., Gebauer,L., Ducker,C.,
            Brockmoller,J. and Sachkova,A.
  TITLE     Substrates of the Human Brain Proton-Organic Cation Antiporter and
            Comparison with Organic Cation Transporter 1 Activities
  JOURNAL   Int J Mol Sci 23 (15), 8430 (2022)
   PUBMED   35955563
  REMARK    GeneRIF: Substrates of the Human Brain Proton-Organic Cation
            Antiporter and Comparison with Organic Cation Transporter 1
            Activities.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 13843)
  AUTHORS   Nakshatri,H., Nakshatri,P. and Currie,R.A.
  TITLE     Interaction of Oct-1 with TFIIB. Implications for a novel response
            elicited through the proximal octamer site of the lipoprotein
            lipase promoter
  JOURNAL   J Biol Chem 270 (33), 19613-19623 (1995)
   PUBMED   7642649
REFERENCE   7  (bases 1 to 13843)
  AUTHORS   Cox,M., van Tilborg,P.J., de Laat,W., Boelens,R., van Leeuwen,H.C.,
            van der Vliet,P.C. and Kaptein,R.
  TITLE     Solution structure of the Oct-1 POU homeodomain determined by NMR
            and restrained molecular dynamics
  JOURNAL   J Biomol NMR 6 (1), 23-32 (1995)
   PUBMED   7663141
REFERENCE   8  (bases 1 to 13843)
  AUTHORS   Roberts,S.B., Segil,N. and Heintz,N.
  TITLE     Differential phosphorylation of the transcription factor Oct1
            during the cell cycle
  JOURNAL   Science 253 (5023), 1022-1026 (1991)
   PUBMED   1887216
REFERENCE   9  (bases 1 to 13843)
  AUTHORS   Hsieh,C.L., Sturm,R., Herr,W. and Francke,U.
  TITLE     The gene for the ubiquitous octamer-binding protein Oct-1 is on
            human chromosome 1, region cen-q32, and near Ly-22 and Ltw-4 on
            mouse chromosome 1
  JOURNAL   Genomics 6 (4), 666-672 (1990)
   PUBMED   2341156
REFERENCE   10 (bases 1 to 13843)
  AUTHORS   Sturm,R.A., Das,G. and Herr,W.
  TITLE     The ubiquitous octamer-binding protein Oct-1 contains a POU domain
            with a homeo box subdomain
  JOURNAL   Genes Dev 2 (12A), 1582-1599 (1988)
   PUBMED   2905684
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX954888.1, X13403.1, BC001664.2, AL136984.20 and AL359962.15.
            
            On Nov 22, 2018 this sequence version replaced NM_002697.3.
            
            Summary: The OCT1 transcription factor was among the first
            identified members of the POU transcription factor family
            (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of
            this family contain the POU domain, a 160-amino acid region
            necessary for DNA binding to the octameric sequence
            ATGCAAAT.[supplied by OMIM, Jul 2010].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.4476890.1,
                                           SRR14038192.434670.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367866.7/ ENSP00000356840.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                BX954888.1         59-80
            23-754              X13403.1           1-732
            755-2572            BC001664.2         728-2545
            2573-6666           AL136984.20        165534-169627
            6667-13843          AL359962.15        2001-9177
FEATURES             Location/Qualifiers
     source          1..13843
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q24.2"
     gene            1..13843
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /note="POU class 2 homeobox 1"
                     /db_xref="GeneID:5451"
                     /db_xref="HGNC:HGNC:9212"
                     /db_xref="MIM:164175"
     exon            1..73
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             13..2313
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Octamer-binding transcription factor-1; POU domain, class
                     2, transcription factor 1; NF-A1; OTF-1; oct-1;
                     octamer-binding protein 1; octamer-binding transcription
                     factor 1"
                     /codon_start=1
                     /product="POU domain, class 2, transcription factor 1
                     isoform 1"
                     /protein_id="NP_002688.3"
                     /db_xref="CCDS:CCDS1259.2"
                     /db_xref="GeneID:5451"
                     /db_xref="HGNC:HGNC:9212"
                     /db_xref="MIM:164175"
                     /translation="MADGGAASQDESSAAAAAAADSRMNNPSETSKPSMESGDGNTGT
                     QTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTSLQAAAQSLNVQSKSNEESG
                     DSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQAQAQLLAAAV
                     QQHSASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQQLQQQNLNLQQFVLV
                     HPTTNLQPAQFIISQTPQGQQGLLQAQNLLTQLPQQSQANLLQSQPSITLTSQPATPT
                     RTIAATPIQTLPQSQSTPKRIDTPSLEEPSDLEELEQFAKTFKQRRIKLGFTQGDVGL
                     AMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAENLSSDSSLSSPSALN
                     SPGIEGLSRRRKKRTSIETNIRVALEKSFLENQKPTSEEITMIADQLNMEKEVIRVWF
                     CNRRQKEKRINPPSSGGTSSSPIKAIFPSPTSLVATTPSLVTSSAATTLTVSPVLPLT
                     SAAVTNLSVTGTSDTTSNNTATVISTAPPASSAVTSPSLSPSPSASASTSEASSASET
                     STTQTTSTPLSSPLGTSQVMVTASGLQTAAAAALQGAAQLPANASLAAMAAAAGLNPS
                     LMAPSQFAAGGALLSLNPGTLSGALSPALMSNSTLATIQALASGGSLPITSLDATGNL
                     VFANAGGAPNIVTAPLFLNPQNLSLLTSNPVSLVSAAAASAGNSAPVASLHATSTSAE
                     SIQNSLFTVASASGAASTTTTASKAQ"
     exon            74..139
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            140..240
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            241..294
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            295..414
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            415..603
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            604..730
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            731..825
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            826..999
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1141
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1281
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..1461
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1567
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1913
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1914..2002
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2003..13843
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      12916..12921
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /note="hexamer: AATAAA"
     polyA_site      12937
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /note="major polyA site"
     regulatory      13818..13823
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /note="hexamer: ATTAAA"
     polyA_site      13843
                     /gene="POU2F1"
                     /gene_synonym="oct-1B; OCT1; Oct1Z; OTF1"
                     /note="major polyA site"
ORIGIN      
        1 aaaatattca aaatggcgga cggaggagca gcgagtcaag atgagagttc agccgcggcg
       61 gcagcagcag cagactcaag aatgaacaat ccgtcagaaa ccagtaaacc atctatggag
      121 agtggagatg gcaacacagg cacacaaacc aatggtctgg actttcagaa gcagcctgtg
      181 cctgtaggag gagcaatctc aacagcccag gcgcaggctt tccttggaca tctccatcag
      241 gtccaactcg ctggaacaag tttacaggct gctgctcagt ctttaaatgt acagtctaaa
      301 tctaatgaag aatcggggga ttcgcagcag ccaagccagc cttcccagca gccttcagtg
      361 caggcagcca ttccccagac ccagcttatg ctagctggag gacagataac tgggcttact
      421 ttgacgcctg cccagcaaca gttactactc cagcaggcac aggcacaggc acagctgctg
      481 gctgctgcag tgcagcagca ctccgccagc cagcagcaca gtgctgctgg agccaccatc
      541 tccgcctctg ctgccacgcc catgacgcag atccccctgt ctcagcccat acagatcgca
      601 caggatcttc aacaactgca acagcttcaa cagcagaatc tcaacctgca acagtttgtg
      661 ttggtgcatc caaccaccaa tttgcagcca gcgcagttta tcatctcaca gacgccccag
      721 ggccagcagg gtctcctgca agcgcaaaat cttctaacgc aactacctca gcaaagccaa
      781 gccaacctcc tacagtcgca gccaagcatc accctcacct cccagccagc aaccccaaca
      841 cgcacaatag cagcaacccc aattcagaca cttccacaga gccagtcaac accaaagcga
      901 attgatactc ccagcttgga ggagcccagt gaccttgagg agcttgagca gtttgccaag
      961 accttcaaac aaagacgaat caaacttgga ttcactcagg gtgatgttgg gctcgctatg
     1021 gggaaactat atggaaatga cttcagccaa actaccatct ctcgatttga agccttgaac
     1081 ctcagcttta agaacatgtg caagttgaag ccacttttag agaagtggct aaatgatgca
     1141 gagaacctct catctgattc gtccctctcc agcccaagtg ccctgaattc tccaggaatt
     1201 gagggcttga gccgtaggag gaagaaacgc accagcatag agaccaacat ccgtgtggcc
     1261 ttagagaaga gtttcttgga gaatcaaaag cctacctcgg aagagatcac tatgattgct
     1321 gatcagctca atatggaaaa agaggtgatt cgtgtttggt tctgtaaccg ccgccagaaa
     1381 gaaaaaagaa tcaacccacc aagcagtggt gggaccagca gctcacctat taaagcaatt
     1441 ttccccagcc caacttcact ggtggcgacc acaccaagcc ttgtgactag cagtgcagca
     1501 actaccctca cagtcagccc tgtcctccct ctgaccagtg ctgctgtgac gaatctttca
     1561 gttacaggca cttcagacac cacctccaac aacacagcaa ccgtgatttc cacagcgcct
     1621 ccagcttcct cagcagtcac gtccccctct ctgagtccct ccccttctgc ctcagcctcc
     1681 acctccgagg catccagtgc cagtgagacc agcacaacac agaccacctc cactcctttg
     1741 tcctcccctc ttgggaccag ccaggtgatg gtgacagcat caggtttgca aacagcagca
     1801 gctgctgccc ttcaaggagc tgcacagttg ccagcaaatg ccagtcttgc tgccatggca
     1861 gctgctgcag gactaaaccc aagcctgatg gcaccctcac agtttgcggc tggaggtgcc
     1921 ttactcagtc tgaatccagg gaccctgagc ggtgctctca gcccagctct aatgagcaac
     1981 agtacactgg caactattca agctcttgct tctggtggct ctcttccaat aacatcactt
     2041 gatgcaactg ggaacctggt atttgccaat gcgggaggag cccccaacat cgtgactgcc
     2101 cctctgttcc tgaaccctca gaacctctct ctgctcacca gcaaccctgt tagcttggtc
     2161 tctgccgccg cagcatctgc agggaactct gcacctgtag ccagccttca cgccacctcc
     2221 acctctgctg agtccatcca gaactctctc ttcacagtgg cctctgccag cggggctgcg
     2281 tccaccacca ccaccgcctc caaggcacag tgagctgggc agagctgggc tgccagaagc
     2341 ctttttcact ctgcagtgtg attggactgc cagccaggtt aataaactga aaaatgtgat
     2401 tggcttcctc tcgccgtgtt gtgagggcaa aggagagaag ggagaaaaaa aaaaaaaaac
     2461 cacacacacc catacacaca taccagaaaa agaaagaaag gatggagacg gaacatttgc
     2521 ctaattttgt aataaaacac tgtcttttca ggattgcttc atggattgga gaactttcta
     2581 accaaaaatt aaaaaaaaaa aaaaaaaaag aaacaaaaaa atcaaaaaca aacaaaaata
     2641 agtgaaagga ctacttatca tttccagcag tcatgatgac aagttaaggt gggttaccaa
     2701 ttccaatata ggaaggggat ttcttgtttg tctaaatttc tttttttctt aaaaaaaaaa
     2761 aatcattttt atgggtctgt tgtacaggcc attaagtcat aacaaggtgt ttataatcgt
     2821 atcaattgtg ttgggggttc ctttcttaac tggtacaatt taggcaggcc tgtattactg
     2881 tattattatt gttgttgttg ttattgtttt atatatatag tttggacatg tttattatct
     2941 cttgctcctg gatcctattt cagtgagctc ccacactgtg gggagggagg gttttggggt
     3001 aaggggagac ttctgttctt aactgtgggg aatgttcttg ctggtttcct tatccttgat
     3061 gactcttaca gaggtgctag aaaattattt tcttttgcca cttatgcaag aggcttggtg
     3121 cagaagctga aggcagtgtg tgcaaacact cccattccac acacgcccct tcccctcccc
     3181 acatcaggtg gtgcctttgg agcacttttg tgtaggaagg aattcctgct gaactgtagc
     3241 tctcactcac ccccaaatca ttgaatgacc ctgaggccca gttcctgtgg tgcaacagtg
     3301 ctgctttctg ctactttcaa tgggaacagc tgtacatgtt gcagggcagg atttggttcc
     3361 caagagcaaa gactactgca gacacctgac ttggtggttc tcctgatttc ttatctcctg
     3421 aaaaatgctc ctgctgttgg tgtgtttgtt tgtttgttcc attttgtgga agtttttcat
     3481 ccaaacttta actaacttgg caaagaaaaa aaactgactt actgaaattg ggaaactttt
     3541 cccttctttt attttctaaa ggaattctac tagactattt tccattgata atatatacaa
     3601 aagaggtgtg atgagaaggg tataaggtta ccaccttttc ctctagagag ccacagactt
     3661 cctttccact ggatggttta taaattcctc taaccctgag attcttcttg gttctctgac
     3721 taaaggaggc acttattagc cgggctggga tctgtagaac ctttgaagaa ggactttgag
     3781 caggaacttt gcacacacct taagtgtctc cttggtttgt tcatgatgct cctgctaggt
     3841 aggtctaaat ggcaatacac atctctttat tcaatgtaga aagtaattag ctctgtaatt
     3901 attttcaggg tgtttgtaga ctatataaat gagcccattg aaaaggaaga gtttgtgaaa
     3961 gtggatgaag aggaataagg gtaacttgga gattaaattc ctttttttgt cagtgtactg
     4021 ttgttatgcc atctgtacca aaaaattttc caaaagactg gatcactgca gtgcagctca
     4081 tctaagttcc ccttcttatt ggagttaaag gttcccattt ggtggctgtg ggtgcttgct
     4141 taaatctctg gtggtcccaa gactgcactt tttctccctt tcgagctggg gatgtagaga
     4201 gaacttacag gtgattttta ttctgattgc ttttgtttgt gtccacaagg gagcttggat
     4261 tctgtgtact tgtctctttc ccattgtcca gtaaggcagt aaatacagac acaatgtgta
     4321 cttttgtgtg tgattcaagt gggagacagt ctgttaaaag gtcagaaaca ttttagtccc
     4381 ctttaaaata tttcttttag tttatttcta tcgtgtttta tttcacagat atttaattac
     4441 cacccctaaa tttcctcctc ttacccttca ccttccctaa aatccttcca aaatcagtga
     4501 actgcaagca gggaaactaa caaatgtcca ttcacccttt ttgtgtggtg tgttttttta
     4561 tgttttgttt tgttttttaa ctaagcttga accaaagtca atttttagca agctgctgta
     4621 ctaatggact agttgataac gtgcagttca gacacatcga ggagatagat gctactgaca
     4681 atttcttttt catttgtctt tattaatttt atatgaaagt tgctgcttgt ttttaatctt
     4741 ttatgttggt aaattgtgat atcctctggg cagacattaa cttgatgttt aactagttta
     4801 ttaggttggt gtgtaaatag aatgacaatg tcaattacta atttctctcc tctccattca
     4861 ctttttctct gttgtagtta aatttaatct tctatcctga cttgacatct ctacctttag
     4921 tctgtggaaa tgattttagg gctatcagta atgctgtagc tgcccataat aatgagtccc
     4981 tcccttggga gtggtgaaac ttagaaggaa ggattcaggg agggaaatgc tggtacccag
     5041 tatactggat tagaagttat agcattaatt tctcaaaatt gctaccaaag gaagtgtgga
     5101 gtcccaaggg actcaagctc agtgaggtaa gacaaagggc aaggaaaggc tgtttgggtg
     5161 gttttttttt ccccacatat ggtttagaaa atgatccctt tcccaaacaa aactcaccaa
     5221 cagactttaa tattagaaaa gatactaagc tttaaatgtc aatacagtgg tcttttattc
     5281 cctgccctgt ctatattttc tactcaattt gggataaggg gattttatgt gaagcacttg
     5341 atgagggtag accagcataa aaaatctgtt taaaagaaga aataatcttt aggaaaagat
     5401 aaatctttag ataaaaataa atttttttct ctttttttac ttatttaaaa ataagttgtg
     5461 aaatgaaagg tacatttaca catgtacaca cgaaacacta atatagcttt ttggtggttg
     5521 tttagtttaa aatcaactcc ctatccatca tccctagata ttctacttat cgtattgctt
     5581 catgaacacc ttagtcattt tttactttac gtatatattt ttttatcatc tcaagaaatg
     5641 cattttgaat tgaatgaact atcttctaag ctaagatact caaatttaac ttttagccac
     5701 cttatatggg agagcctgga aactaaaggg gaccagggac catttgacca aaagaggtgt
     5761 ttggtggagt ttctgcattt caaagaagaa aggccagata ggtcatcaca attcattgta
     5821 ttattctttt tttttcccct taatgtttat gcaaatttta ttttatattt ctgaatttgg
     5881 ttagcacata ctaagccaaa gactattcta aatgcattta tagtgaatac tatgtatgcc
     5941 catggtaatg ggtcctccct aaggagtggt gaatgtaatt gtgtgaatcc tgcttatcac
     6001 aagtggtgca atttggtcat aaactttatt tataccctgt atacatctga ataacagagc
     6061 agaaagacct aaaaaagggc ttctcacagg aacaatatta ctatgttgtt ctaagaaaaa
     6121 ataagttgag tgctgtatta gtttaagtgt ttctaaagtt ctataggcag cattttagaa
     6181 ttaagaattg tgtatatcca ttaactgata taattcacat gtccttttat ttgttatcag
     6241 aaagatcaat actatcccaa tttattcaac ttgttaccca agaaatggaa ccaaaggaat
     6301 ccaactttca ttttgtgtag aatcatacct acttatactt aatgctagca ctgtaagaaa
     6361 taagtctttt ttgtttaaaa aagggaagat aagattaaag attcttagtg agatcatctt
     6421 gccaatttgt tgtacatctc tcattcattg ttgggggaaa aaaaagcaca actatacctc
     6481 tttaatgtta ttttcttcca ttatccctca tttatttgga aagataaggg aaaccttgtc
     6541 tgtattatcc agaagcacag atatttgctt gacacaatat ttaaattcag cccaatcttt
     6601 aacattaaaa aattttcttt ttaacttgct ggaaaagttg gatacattgg caaatatagg
     6661 gaattcatgt cttttttttt tttttttttc tggtcttgct ctgttgccta ggctagagtg
     6721 caacggcatg atctcggctc actgcaacct ctgcctcccg ggttcaagca attctcctgc
     6781 ttcagcctcc caagtagctg ggattacagg cgcccaccac cgcacccggc taatttttgt
     6841 atttttagta gggacgggat ttctccgtgt tggccaggct ttttgaactc ctgaccttag
     6901 gtgatctgcc tgccttggcc tcccaaagtg ctgggattac aggtatgagc cactgtgccc
     6961 atcctcatgt caatttttaa agtgataaat cctgatatta tacattgcaa ttagtgtaga
     7021 ataaacgctt ggcttataga actctctgtt cttagtctaa agctgttccc aaaccaggtc
     7081 atcctcacga aacattcttg accaagaaaa aagataaggt cattgagata ggaagacaga
     7141 ggaaaagcct cttgctgttg tttcttccca agaaaggaga agccctgcca gggagaagtc
     7201 agtagtattg ctgactcact gtatcactga gtgtagggtg tggtagcaag gaggaggcag
     7261 gggattcacg ctgacaggtg gctctggcct ggctcttggg gggccttctg aagaccagtc
     7321 tgcagtttga ggaagggccc caacaattca tttggagagc ttggccaaat actttctcat
     7381 taaatcagca cctaaacttg atgaacttaa agtttgttta ttagagttga aaacattaaa
     7441 agatgactga tactaatggg agaaaaatac taaagaccca aaatttaaaa ttttccaaaa
     7501 tggacaagaa ttgatgggtt aatttgctca taactgatat gagtttgctt ttatgtgtgt
     7561 gtggaaagca tttaatgtgt gatgctgttc atagtcctgg ctatttgatg tcattttcag
     7621 ttttacaaca ttttgaggat gacactttat aaaatggtaa atctttctca gcgtttacaa
     7681 acttggcttt tgagaaggga aaaatcctct ttgctatttc tgtaagaaga ataactgaga
     7741 tcagctatta tataggtttg catggatggt gtccacaata caaatgtaaa cttggacagg
     7801 aataggataa ataaattgct ttcatcataa aggccaatca tccatttctt agtccaagga
     7861 gattaaatat aaataggaca cccctgagat ttccagtaag gtatttgaaa ggattccact
     7921 gggagggcag ggaatatatt ttttctcaga gcatccagtt tgtacccagt tttctatatg
     7981 gtttgtgaac tgtagctttt aaaaaaaaat tttgtagttt tcttctaacc ttattcatta
     8041 agtagttcct tgttttggcc cttctactct tggccttagc taaaagcagt aaatgtgagt
     8101 gaattttatg ttgattatgt cacctggatc tcctttcctt cccttctttt tctcattgaa
     8161 tggtggtacc ttaatttggg taacagctag cttctggcac cttcataaaa tacctcagaa
     8221 tagcttagca ttgttgcaaa actggtttta aactaaaaac caaataaata agtgaaaaaa
     8281 ttttataaat agtggaaata attctagctg tagcatttag ttgaactaat gtttattatg
     8341 atcgataaac aagattgatg ctgtatgtat ttgggatcct gtttataggt tacaatttat
     8401 agggttaggg agggttgggt gggggaagag gagatgttga ttatgttaga aggaaaacat
     8461 tgctcgtgtg tgtgtttgtt ttttcttata tctgtcttgc caaaggaaac ttgattttcc
     8521 ctgtgattgg ctttggcctc tgtggtctgg tctgcagcag tgtgtggtgg catgcaggta
     8581 acatatgggg tgtccagtag gttcagggac cctgagggtg catttacatc ccttcctatg
     8641 gttgcccatg ccccccagac cctgccaacc tctcctaatg attcattacc tctgtacata
     8701 gcaaaagcat aggcaaagga tgaatgcgtg aatgcctgga tgtgcagttg agaaacagag
     8761 gcagacatag acatgcacca attaatttgg agccaagagg tgagtgattg atgtatctta
     8821 aatctaggaa agttaaagtg aaacaggttc tttgtgttgc cctctgtcaa gttactgaat
     8881 gttgtgctaa taacacaaca ctttaaaacc ttacttactt ttctcatacc aaggctagtt
     8941 ctttctagac caaaaacaca aacaggataa aaagcaaacc aagaagttga aagctaacct
     9001 gcccacctca cccctgtgat acaaagtgtg aaccttaagg attagttggt gggattgagg
     9061 agagaattat tcttggtggg aacatgaatg agaaatgatg ctggcttagg aaaaatcaca
     9121 agccctttct gcccatactc tgggacaaac tttcttggct ccctgagttc ggatatagac
     9181 tgtatctcta gaaaggctat ttttttaaat aaatgatccc acagaactta agtgggattt
     9241 ggtttagata ccaaagacac tgctaatgtc attgcttatc cttaagtttt caaaacaggc
     9301 agagggaata ggagcaaaaa gccaaaattt ggaaaagctt tactttatct ctttccttcc
     9361 cttttcccct tttcccagtc ccctgctttt tgaaggaagt cttcttggtt atcctggctt
     9421 tggaaaagag atttattttg ttttgctctg ctggctcttt aaagatggat agttgctcaa
     9481 tctagcagtg atgttcttgg aattgctgag aaatttgggg agggcaaaag ataggggtag
     9541 aattttttca ttatttccct ttatctaata cttttaaata gaaccaacac agcctatatg
     9601 agttcagaca atatttagat gtggtatctc catctgtctc ctgtaaaaga taagaatttt
     9661 caagaacagg attacgtgga aaaccaaaag atcttccctt actctcctat aaatgttttg
     9721 ttccaaatgt ttttatatat gggctctagg gagtcaggta gttcattgtt tcggtgtact
     9781 attgatagga cacagaaagg gagagagggt aaagaaatgt atcactctct gaatattgca
     9841 tcaaaaatga ttaggttgca gaacttcatg aaagctttac taataatctt attgttctga
     9901 cattatgtaa aggtggtatt aatattgtat gacttgtcaa agtgctagat aggtttataa
     9961 gtaggtaata gggatgttga agattagagc acttgaaaca gaagttctgg gaaaacaagg
    10021 tgtgtgtaat ggaacaccac tttgagcaca gaaacaaagg tcccttggac ctggtaggga
    10081 agatgtgatt tatgattgtt tctgtgtccc tgtatctgcc caccctgcac agggctccac
    10141 cacccagggc caccttctgg tttaaaccca ggacactgtc aaaaagttaa gaccccaaaa
    10201 ctaatttact gtaaaaaaca ttgaggactg ctgcaaagtt ttcccttgtt ttctttgtgt
    10261 acttgttcat cattgtataa gttagccaca gcttcacaag agcagcttaa ggcttctttc
    10321 ataaattggc agtggcattg agttctcaaa cattatatcc caaagtctgc aggcagacag
    10381 ctggatacag cgctgtgtat aaatgagacg tccaaacact tgagtttctt aagattggga
    10441 tctctttcaa atgaaaaagg acagagccaa gtagagaaaa gactttgtgc tcccacccag
    10501 ccttaatgag tctcatggtc taaaagtata ggaagaaatg aaatgcactt tcagaagcta
    10561 aaatgacagt gtctgctaca aaaggctgta gttgtaggca gtcggggatg ccatgtcctt
    10621 ggttccattc ctgcgtgagt ctgcagaagg cacacacttt gtaagagtag agtggactag
    10681 tgccagcctg aataggttta aaactgcaaa cagttggaga acatggaaca ggttggtgca
    10741 ggaagcctaa gattttgcaa tcatattata acattggctt ttgacaacat aaatgttgta
    10801 tcttccctaa ggtcaggtcg gggaaagaaa gacttccagc ttcttacctc tgcgtgcatg
    10861 ggcacgtgtg cattgctcag tccgcaggag gtctcactcc gcaggaaacg ctctcctccc
    10921 gcataagtct gtacttccat cccctcatct gtggtagtag tgaaggctag gtgagtaagc
    10981 gtgggctgtt ctacccacca gaagtccagg agctgttgta tacctcattt ctaactcgtg
    11041 accgagtgac ttgctttaac tttctcgaaa tcctacagag ttgccaagtc tgccctccct
    11101 cctcagtcat gttaaactct ggcctatagc atcatgggac ctgtagccta gggtgggacc
    11161 ccctaaagcc tctgaatgtc gctgcttaaa agctactgca aactgagggc aaattgcaat
    11221 cttctattcc tttttgttgc aaggggtctt cacaggtctc ttaacatctg ctttccctgc
    11281 caccctgcct ttaggggctg gccagctatc cacaccccta acccaccctg tgtttctgac
    11341 agctggccac acgtcaactt ctgtacttgc cttttccttg gtggggtaga ggccaacccc
    11401 ttctcctctg aggcctcagg gttctgtttc ttttcaggac tttgggtaga agggaagaca
    11461 ccaaaggctc ctttaagctg actgctgcat acacatttca ctttttttcc tttgacatga
    11521 ccaaaaatat ccaaatattt aagtttatta ttattattat tattatttga caatcttata
    11581 tccaatgggc tctctagaag ctgcatcccc agccctttca ccttttcttt gaatgtagtt
    11641 cccaaccttc aattcccacc cctaatgctg acaaaaagct tagccaggtc atgatactgc
    11701 tgctataagc caggggaggg tggtttcttt gttttgtttt gttttgtttt gtttttcaaa
    11761 tttccagcca aaaccaaaga tttcttaatg attgtataaa ctcaaaacaa acaaaagaac
    11821 caaagaaaag ggaagtcttc agcatcagtc cagtctgtgg tgtcctggca cttagaagag
    11881 tgaggccagg gaaggtgggg cggggggacc ctctgactgg atcagaaagg gcaggcaagc
    11941 tgcttaccac cttccagggt ccagcacagg tgcatgcccc tagctttggc cctcacaaac
    12001 ttgtgagcat gccctgaaac aaattggcat ttcagatttt ccaaaacaaa agtcaaaaaa
    12061 ccccatcacc accatcatcc accctacacc aaccagggtc actatgggga cttagcacag
    12121 ggcctaggga gggtgggagc tgctgctgga ctggcccgag gactccacag agtgggggac
    12181 gttgaagaca ctggcgcacg attggctgct ctgctgggtg ctttaacctc tggggctggg
    12241 cgaatgcacg ccacggtgcc cttctgagcc tttttttctt ttcctgtact ggaactgctt
    12301 ggaagtggct gtcctgtttg tgagaaaaca tgcagaaaga ttatcaagct tttcatttct
    12361 ctttattgta gttttctttc ctttttgcag caccactgtg caactatgca ttgtacttca
    12421 caaccttttg tgctaggtag atgcctgtga ctttttaact tggggggtgg ggggattgca
    12481 aatgaaggaa ctttttacat ggatcttttt aatctcagtt ggttggggga ggggggtact
    12541 tggttcttgg gtcacacaag ctctatccca aagcaaaatc caaatgttaa taatattagc
    12601 ctgatgcaga taccaaagat aatttctttc ctgcagaacc ttagactcgt gtattaagta
    12661 cgattaccca gcacttgcat tagtctaacc tcagtaattc caaagcttag atgtatattt
    12721 tggtatattt ctgggatatt aaaaaaaaaa tgtcgtttaa cttcttgttt gtttcagtgt
    12781 aatgctctta aagtcatagc atgaaaataa ttgaactgtc ctattcttag tagtttgaaa
    12841 taatagtatt tttgtatgtt ttgggtgtgt ctatgtatgt attaacataa cagttttcac
    12901 tgcctaggtg ttcataataa aaaagaaaat gaaaaaagtt ctgagtgtac ataatattca
    12961 ataataatct cccaccaggt gtcaatttag attgcatatt cctcattact gttcccaact
    13021 gggattttgt tttcttttaa agagacagtg atcgtgtttt tctaaagatg ttttctttct
    13081 ctctttcaat atcttaactc actttttttt tcttcctttt ttgtcttaat tattaattca
    13141 ggggtgtttg tccactgttg tcaaaggagg ggcacaaggg gaattggccc ccggcctcct
    13201 agaacttttt gttttatgta ctttaaaatg tgagtttgag ttcttctttg tggaaactta
    13261 agatgtggtg taaatgattc tttccaaaat tgtccagcag cttgaatgag gcagtgacaa
    13321 ttctgaggtg gtttattggg agtccttcta catttctctt agataactct gcactctggg
    13381 tggctattgt gtagccttac tgccccagag tgcctgttag ccaaatattt cccctctgat
    13441 aggaatattt tctaagaatc agctgataac ttgcgtgctg gaccttgtta tctgtgcccc
    13501 tgggagacac acgttttctt ggttttgaaa acctgaaaca caggcaactt tacattttgg
    13561 ggaattagct gatgcctcct gaagcctgag gaggtggcgg ggaatatgag cggtgctgtc
    13621 tctctcaaaa gtgcccttta gatgattccc cctcctaggg ctgcctgcag gggctgtagg
    13681 cttgggaaag attgtgtagg tgacagtgaa tcagaatgaa gtggtagatt ttgtgtagat
    13741 gcatttgtct gctgtaattt tttatatata tattaaactt actgtaactg tacagttcat
    13801 ttctgttgta aaacatcatt aaaccatctt ccaagtgttt tca
//
