LOCUS       NM_001395413            2446 bp    mRNA    linear   PRI 05-OCT-2024
DEFINITION  Homo sapiens cytochrome p450 oxidoreductase (POR), transcript
            variant 2, mRNA.
ACCESSION   NM_001395413 NM_000941
VERSION     NM_001395413.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Burris-Hiday,S.D. and Scott,E.E.
  TITLE     Allosteric modulation of cytochrome P450 enzymes by the NADPH
            cytochrome P450 reductase FMN-containing domain
  JOURNAL   J Biol Chem 299 (9), 105112 (2023)
   PUBMED   37517692
  REMARK    GeneRIF: Allosteric modulation of cytochrome P450 enzymes by the
            NADPH cytochrome P450 reductase FMN-containing domain.
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Esteves,F., Almeida,C.M.M., Silva,S., Saldanha,I., Urban,P.,
            Rueff,J., Pompon,D., Truan,G. and Kranendonk,M.
  TITLE     Single Mutations in Cytochrome P450 Oxidoreductase Can Alter the
            Specificity of Human Cytochrome P450 1A2-Mediated Caffeine
            Metabolism
  JOURNAL   Biomolecules 13 (7), 1083 (2023)
   PUBMED   37509119
  REMARK    GeneRIF: Single Mutations in Cytochrome P450 Oxidoreductase Can
            Alter the Specificity of Human Cytochrome P450 1A2-Mediated
            Caffeine Metabolism.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2446)
  AUTHORS   Nakagawa,J., Kinjo,T., Aiuchi,N., Ueno,K., Tomita,H. and Niioka,T.
  TITLE     Effect of pregnane X receptor and cytochrome P450 oxidoreductase
            gene polymorphisms on trough concentrations of rivaroxaban and
            edoxaban in patients with nonvalvular atrial fibrillation
  JOURNAL   Eur J Clin Pharmacol 79 (5), 703-705 (2023)
   PUBMED   36951964
  REMARK    GeneRIF: Effect of pregnane X receptor and cytochrome P450
            oxidoreductase gene polymorphisms on trough concentrations of
            rivaroxaban and edoxaban in patients with nonvalvular atrial
            fibrillation.
REFERENCE   4  (bases 1 to 2446)
  AUTHORS   Pandey,A.V. and Sproll,P.
  TITLE     Pharmacogenomics of human P450 oxidoreductase
  JOURNAL   Front Pharmacol 5, 103 (2014)
   PUBMED   24847272
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2446)
  AUTHORS   Pandey,A.V. and Fluck,C.E.
  TITLE     NADPH P450 oxidoreductase: structure, function, and pathology of
            diseases
  JOURNAL   Pharmacol Ther 138 (2), 229-254 (2013)
   PUBMED   23353702
  REMARK    GeneRIF: Structural, enzymatic and clinical implications of the
            NADPH P450 oxidoreductase mutations linked to newly identified
            disorders in humans, now categorized as POR deficiency[review].
            Review article
REFERENCE   6  (bases 1 to 2446)
  AUTHORS   Wilks,A., Black,S.M., Miller,W.L. and Ortiz de Montellano,P.R.
  TITLE     Expression and characterization of truncated human heme oxygenase
            (hHO-1) and a fusion protein of hHO-1 with human cytochrome P450
            reductase
  JOURNAL   Biochemistry 34 (13), 4421-4427 (1995)
   PUBMED   7703255
REFERENCE   7  (bases 1 to 2446)
  AUTHORS   Idkowiak,J., Cragun,D., Hopkin,R.J. and Arlt,W.
  TITLE     Cytochrome P450 Oxidoreductase Deficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301592
REFERENCE   8  (bases 1 to 2446)
  AUTHORS   Shephard,E.A., Palmer,C.N., Segall,H.J. and Phillips,I.R.
  TITLE     Quantification of cytochrome P450 reductase gene expression in
            human tissues
  JOURNAL   Arch Biochem Biophys 294 (1), 168-172 (1992)
   PUBMED   1550342
REFERENCE   9  (bases 1 to 2446)
  AUTHORS   Haniu,M., McManus,M.E., Birkett,D.J., Lee,T.D. and Shively,J.E.
  TITLE     Structural and functional analysis of NADPH-cytochrome P-450
            reductase from human liver: complete sequence of human enzyme and
            NADPH-binding sites
  JOURNAL   Biochemistry 28 (21), 8639-8645 (1989)
   PUBMED   2513880
REFERENCE   10 (bases 1 to 2446)
  AUTHORS   Shephard,E.A., Phillips,I.R., Santisteban,I., West,L.F.,
            Palmer,C.N., Ashworth,A. and Povey,S.
  TITLE     Isolation of a human cytochrome P-450 reductase cDNA clone and
            localization of the corresponding gene to chromosome 7q11.2
  JOURNAL   Ann Hum Genet 53 (4), 291-301 (1989)
   PUBMED   2516426
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005067.2 and AC006330.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jul 8, 2021 this sequence version replaced NM_000941.3.
            
            Summary: This gene encodes an endoplasmic reticulum membrane
            oxidoreductase that is essential for multiple metabolic processes,
            including reactions catalyzed by cytochrome P450 proteins for
            metabolism of steroid hormones, drugs and xenobiotics. The encoded
            protein has a flavin adenine dinucleotide (FAD)-binding domain and
            a flavodoxin-like domain which bind two cofactors, FAD and FMN,
            that allow it to donate electrons directly from NADPH to all
            microsomal P450 enzymes. Mutations in this gene cause a complex set
            of disorders, including apparent combined P450C17 and P450C21
            deficiency, amenorrhea and disordered steroidogenesis, congenital
            adrenal hyperplasia and Antley-Bixler syndrome, that resemble those
            caused by defects in steroid metabolizing enzymes such as
            aromatase, 21-hydroxylase, and 17 alpha-hydroxylase. [provided by
            RefSeq, Aug 2020].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853564.24700.1,
                                           SRR11853563.30382.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000461988.6/
                                                ENSP00000419970.2
            RefSeq Select criteria           :: based on manual assertion,
                                                conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                AC005067.2         41278-41302         c
            26-217              AC005067.2         2277-2468           c
            218-266             AC006330.5         80095-80143         c
            267-395             AC006330.5         72977-73105         c
            396-545             AC006330.5         72068-72217         c
            546-670             AC006330.5         71384-71508         c
            671-760             AC006330.5         70950-71039         c
            761-859             AC006330.5         70234-70332         c
            860-976             AC006330.5         68920-69036         c
            977-1095            AC006330.5         68700-68818         c
            1096-1277           AC006330.5         67598-67779         c
            1278-1427           AC006330.5         67349-67498         c
            1428-1698           AC006330.5         66707-66977         c
            1699-1844           AC006330.5         66488-66633         c
            1845-1927           AC006330.5         66315-66397         c
            1928-2446           AC006330.5         65701-66219         c
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q11.23"
     gene            1..2446
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /note="cytochrome p450 oxidoreductase"
                     /db_xref="GeneID:5447"
                     /db_xref="HGNC:HGNC:9208"
                     /db_xref="MIM:124015"
     exon            1..25
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            26..217
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     CDS             39..2072
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /EC_number="1.6.2.4"
                     /note="isoform 1 is encoded by transcript variant 2;
                     NADPH--cytochrome P450 reductase; NADPH-dependent
                     cytochrome P450 reductase; P450 (cytochrome)
                     oxidoreductase; NADPH--hemoprotein reductase"
                     /codon_start=1
                     /product="NADPH--cytochrome P450 reductase isoform 1"
                     /protein_id="NP_001382342.1"
                     /db_xref="CCDS:CCDS5579.2"
                     /db_xref="GeneID:5447"
                     /db_xref="HGNC:HGNC:9208"
                     /db_xref="MIM:124015"
                     /translation="MGDSHVDTSSTVSEAVAEEVSLFSMTDMILFSLIVGLLTYWFLF
                     RKKKEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDA
                     HRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETD
                     VDLSGVKFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDFIT
                     WREQFWPAVCEHFGVEATGEESSIRQYELVVHTDIDAAKVYMGEMGRLKSYENQKPPF
                     DAKNPFLAAVTTNRKLNQGTERHLMHLELDISDSKIRYESGDHVAVYPANDSALVNQL
                     GKILGADLDVVMSLNNLDEESNKKHPFPCPTSYRTALTYYLDITNPPRTNVLYELAQY
                     ASEPSEQELLRKMASSSGEGKELYLSWVVEARRHILAILQDCPSLRPPIDHLCELLPR
                     LQARYYSIASSSKVHPNSVHICAVVVEYETKAGRINKGVATNWLRAKEPAGENGGRAL
                     VPMFVRKSQFRLPFKATTPVIMVGPGTGVAPFIGFIQERAWLRQQGKEVGETLLYYGC
                     RRSDEDYLYREELAQFHRDGALTQLNVAFSREQSHKVYVQHLLKQDREHLWKLIEGGA
                     HIYVCGDARNMARDVQNTFYDIVAELGAMEHAQAVDYIKKLMTKGRYSLDVWS"
     exon            218..266
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            267..395
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            396..545
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            546..670
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            671..760
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            761..859
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            860..976
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1095
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1277
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1427
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1698
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            1699..1844
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..1927
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..2446
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2426..2431
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /note="hexamer: AATAAA"
     polyA_site      2446
                     /gene="POR"
                     /gene_synonym="CPR; CYPOR; P450R"
                     /note="major polyA site"
ORIGIN      
        1 gcagagagca gccgggctgc cagcgtttca tgatcaacat gggagactcc cacgtggaca
       61 ccagctccac cgtgtccgag gcggtggccg aagaagtatc tcttttcagc atgacggaca
      121 tgattctgtt ttcgctcatc gtgggtctcc taacctactg gttcctcttc agaaagaaaa
      181 aagaagaagt ccccgagttc accaaaattc agacattgac ctcctctgtc agagagagca
      241 gctttgtgga aaagatgaag aaaacgggga ggaacatcat cgtgttctac ggctcccaga
      301 cggggactgc agaggagttt gccaaccgcc tgtccaagga cgcccaccgc tacgggatgc
      361 gaggcatgtc agcggaccct gaggagtatg acctggccga cctgagcagc ctgccagaga
      421 tcgacaacgc cctggtggtt ttctgcatgg ccacctacgg tgagggagac cccaccgaca
      481 atgcccagga cttctacgac tggctgcagg agacagacgt ggatctctct ggggtcaagt
      541 tcgcggtgtt tggtcttggg aacaagacct acgagcactt caatgccatg ggcaagtacg
      601 tggacaagcg gctggagcag ctcggcgccc agcgcatctt tgagctgggg ttgggcgacg
      661 acgatgggaa cttggaggag gacttcatca cctggcgaga gcagttctgg ccggccgtgt
      721 gtgaacactt tggggtggaa gccactggcg aggagtccag cattcgccag tacgagcttg
      781 tggtccacac cgacatagat gcggccaagg tgtacatggg ggagatgggc cggctgaaga
      841 gctacgagaa ccagaagccc ccctttgatg ccaagaatcc gttcctggct gcagtcacca
      901 ccaaccggaa gctgaaccag ggaaccgagc gccacctcat gcacctggaa ttggacatct
      961 cggactccaa aatcaggtat gaatctgggg accacgtggc tgtgtaccca gccaacgact
     1021 ctgctctcgt caaccagctg ggcaaaatcc tgggtgccga cctggacgtc gtcatgtccc
     1081 tgaacaacct ggatgaggag tccaacaaga agcacccatt cccgtgccct acgtcctacc
     1141 gcacggccct cacctactac ctggacatca ccaacccgcc gcgtaccaac gtgctgtacg
     1201 agctggcgca gtacgcctcg gagccctcgg agcaggagct gctgcgcaag atggcctcct
     1261 cctccggcga gggcaaggag ctgtacctga gctgggtggt ggaggcccgg aggcacatcc
     1321 tggccatcct gcaggactgc ccgtccctgc ggccccccat cgaccacctg tgtgagctgc
     1381 tgccgcgcct gcaggcccgc tactactcca tcgcctcatc ctccaaggtc caccccaact
     1441 ctgtgcacat ctgtgcggtg gttgtggagt acgagaccaa ggctggccgc atcaacaagg
     1501 gcgtggccac caactggctg cgggccaagg agcctgccgg ggagaacggc ggccgtgcgc
     1561 tggtgcccat gttcgtgcgc aagtcccagt tccgcctgcc cttcaaggcc accacgcctg
     1621 tcatcatggt gggccccggc accggggtgg cacccttcat aggcttcatc caggagcggg
     1681 cctggctgcg acagcagggc aaggaggtgg gggagacgct gctgtactac ggctgccgcc
     1741 gctcggatga ggactacctg taccgggagg agctggcgca gttccacagg gacggtgcgc
     1801 tcacccagct caacgtggcc ttctcccggg agcagtccca caaggtctac gtccagcacc
     1861 tgctaaagca agaccgagag cacctgtgga agttgatcga aggcggtgcc cacatctacg
     1921 tctgtgggga tgcacggaac atggccaggg atgtgcagaa caccttctac gacatcgtgg
     1981 ctgagctcgg ggccatggag cacgcgcagg cggtggacta catcaagaaa ctgatgacca
     2041 agggccgcta ctccctggac gtgtggagct aggggcctgc ctgccccacc caccccacag
     2101 actccggcct gtaatcagct ctcctggctc cctcccgtag tctcctgggt gtgtttggct
     2161 tggccttggc atgggcgcag gcccagtgac aaagactcct ctgggcctgg ggtgcatcct
     2221 cctcagcccc caggccaggt gaggtccacc ggcccctggc agcacagccc agggcctgca
     2281 tgggggcacc gggctccatg cctctggagg cctctggccc tcggtggctg cacagaaggg
     2341 ctctttctct ctgctgagct gggcccagcc cctccacgtg atttccagtg agtgtaaata
     2401 attttaaata acctctggcc cttggaataa agttctgttt tctgta
//
