LOCUS       NM_016310               1372 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens RNA polymerase III subunit K (POLR3K), mRNA.
ACCESSION   NM_016310
VERSION     NM_016310.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Hou,H., Li,Y., Wang,M., Liu,A., Yu,Z., Chen,K., Zhao,D. and Xu,Y.
  TITLE     Structural insights into RNA polymerase III-mediated transcription
            termination through trapping poly-deoxythymidine
  JOURNAL   Nat Commun 12 (1), 6135 (2021)
   PUBMED   34675218
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Girbig,M., Misiaszek,A.D., Vorlander,M.K., Lafita,A., Grotsch,H.,
            Baudin,F., Bateman,A. and Muller,C.W.
  TITLE     Cryo-EM structures of human RNA polymerase III in its unbound and
            transcribing states
  JOURNAL   Nat Struct Mol Biol 28 (2), 210-219 (2021)
   PUBMED   33558764
  REMARK    GeneRIF: Cryo-EM structures of human RNA polymerase III in its
            unbound and transcribing states.
REFERENCE   3  (bases 1 to 1372)
  AUTHORS   Ramsay,E.P., Abascal-Palacios,G., Daiss,J.L., King,H., Gouge,J.,
            Pilsl,M., Beuron,F., Morris,E., Gunkel,P., Engel,C. and Vannini,A.
  TITLE     Structure of human RNA polymerase III
  JOURNAL   Nat Commun 11 (1), 6409 (2020)
   PUBMED   33335104
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1372)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1372)
  AUTHORS   Hasegawa,Y., Taylor,D., Ovchinnikov,D.A., Wolvetang,E.J., de
            Torrente,L. and Mar,J.C.
  TITLE     Variability of Gene Expression Identifies Transcriptional
            Regulators of Early Human Embryonic Development
  JOURNAL   PLoS Genet 11 (8), e1005428 (2015)
   PUBMED   26288249
  REMARK    GeneRIF: Results from a study on gene expression variability
            markers in early-stage human embryos shows that POLR3K is a
            putative expression variability marker for the 3-day, 8-cell embryo
            stage.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1372)
  AUTHORS   Daniels,R.J., Peden,J.F., Lloyd,C., Horsley,S.W., Clark,K.,
            Tufarelli,C., Kearney,L., Buckle,V.J., Doggett,N.A., Flint,J. and
            Higgs,D.R.
  TITLE     Sequence, structure and pathology of the fully annotated terminal 2
            Mb of the short arm of human chromosome 16
  JOURNAL   Hum Mol Genet 10 (4), 339-352 (2001)
   PUBMED   11157797
REFERENCE   7  (bases 1 to 1372)
  AUTHORS   Chedin,S., Riva,M., Schultz,P., Sentenac,A. and Carles,C.
  TITLE     The RNA cleavage activity of RNA polymerase III is mediated by an
            essential TFIIS-like subunit and is important for transcription
            termination
  JOURNAL   Genes Dev 12 (24), 3857-3871 (1998)
   PUBMED   9869639
REFERENCE   8  (bases 1 to 1372)
  AUTHORS   Spakovskii,G.V. and Lebedenko,E.N.
  TITLE     [Molecular identification and characteristics of hRPC11, the
            smallest specific subunit of human RNA polymerase III]
  JOURNAL   Bioorg Khim 24 (11), 877-880 (1998)
   PUBMED   10079944
REFERENCE   9  (bases 1 to 1372)
  AUTHORS   Flint,J., Thomas,K., Micklem,G., Raynham,H., Clark,K.,
            Doggett,N.A., King,A. and Higgs,D.R.
  TITLE     The relationship between chromosome structure and function at a
            human telomeric region
  JOURNAL   Nat Genet 15 (3), 252-257 (1997)
   PUBMED   9054936
REFERENCE   10 (bases 1 to 1372)
  AUTHORS   Jang,K.L., Collins,M.K. and Latchman,D.S.
  TITLE     The human immunodeficiency virus tat protein increases the
            transcription of human Alu repeated sequences by increasing the
            activity of the cellular transcription factor TFIIIC
  JOURNAL   J Acquir Immune Defic Syndr (1988) 5 (11), 1142-1147 (1992)
   PUBMED   1403646
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from KF456133.1, BC011932.2,
            DR978472.1 and Z69719.1.
            
            On May 17, 2019 this sequence version replaced NM_016310.4.
            
            Summary: This gene encodes a small essential subunit of RNA
            polymerase III, the polymerase responsible for synthesizing
            transfer and small ribosomal RNAs in eukaryotes. The
            carboxy-terminal domain of this subunit shares a high degree of
            sequence similarity to the carboxy-terminal domain of an RNA
            polymerase II elongation factor. This similarity in sequence is
            supported by functional studies showing that this subunit is
            required for proper pausing and termination during transcription.
            Pseudogenes of this gene are found on chromosomes 13 and
            17.[provided by RefSeq, Jul 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF060223.1, SRR1803613.29839.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000293860.6/ ENSP00000293860.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                KF456133.1         85-99               c
            16-730              BC011932.2         1-715
            731-800             DR978472.1         33-102
            801-1372            Z69719.1           9101-9672           c
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..1372
                     /gene="POLR3K"
                     /gene_synonym="C11; C11-RNP3; HLD21; My010; RPC10; RPC11;
                     RPC12.5"
                     /note="RNA polymerase III subunit K"
                     /db_xref="GeneID:51728"
                     /db_xref="HGNC:HGNC:14121"
                     /db_xref="MIM:606007"
     exon            1..133
                     /gene="POLR3K"
                     /gene_synonym="C11; C11-RNP3; HLD21; My010; RPC10; RPC11;
                     RPC12.5"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..349
                     /gene="POLR3K"
                     /gene_synonym="C11; C11-RNP3; HLD21; My010; RPC10; RPC11;
                     RPC12.5"
                     /EC_number="2.7.7.6"
                     /note="RNA polymerase III subunit CII; RNA polymerase III
                     subunit C10; RNA polymerase III subunit C11; DNA-directed
                     RNA polymerase III subunit K; DNA-directed RNA polymerases
                     III 12.5 kDa polypeptide; RNA polymerase III 12.5 kDa
                     subunit; polymerase (RNA) III subunit K; polymerase (RNA)
                     III (DNA directed) polypeptide K, 12.3 kDa"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase III subunit RPC10"
                     /protein_id="NP_057394.3"
                     /db_xref="CCDS:CCDS10395.1"
                     /db_xref="GeneID:51728"
                     /db_xref="HGNC:HGNC:14121"
                     /db_xref="MIM:606007"
                     /translation="MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYP
                     KLKEVDDVLGGAAAWENVDSTAESCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQC
                     GHRWRD"
     exon            134..221
                     /gene="POLR3K"
                     /gene_synonym="C11; C11-RNP3; HLD21; My010; RPC10; RPC11;
                     RPC12.5"
                     /inference="alignment:Splign:2.1.0"
     exon            222..1372
                     /gene="POLR3K"
                     /gene_synonym="C11; C11-RNP3; HLD21; My010; RPC10; RPC11;
                     RPC12.5"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      730
                     /gene="POLR3K"
                     /gene_synonym="C11; C11-RNP3; HLD21; My010; RPC10; RPC11;
                     RPC12.5"
                     /note="major polyA site"
     regulatory      781..786
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR3K"
                     /gene_synonym="C11; C11-RNP3; HLD21; My010; RPC10; RPC11;
                     RPC12.5"
                     /note="hexamer: AATGAA"
     polyA_site      794
                     /gene="POLR3K"
                     /gene_synonym="C11; C11-RNP3; HLD21; My010; RPC10; RPC11;
                     RPC12.5"
     regulatory      863..868
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR3K"
                     /gene_synonym="C11; C11-RNP3; HLD21; My010; RPC10; RPC11;
                     RPC12.5"
                     /note="hexamer: AATAGA"
     polyA_site      887
                     /gene="POLR3K"
                     /gene_synonym="C11; C11-RNP3; HLD21; My010; RPC10; RPC11;
                     RPC12.5"
     polyA_site      1015
                     /gene="POLR3K"
                     /gene_synonym="C11; C11-RNP3; HLD21; My010; RPC10; RPC11;
                     RPC12.5"
ORIGIN      
        1 ggagcctgcg gagttcgaga ccatgctgct gttctgcccc ggctgcggga acgggctgat
       61 cgtggaggag ggacaacgct gccaccgctt cgcctgcaac acgtgcccct acgtgcacaa
      121 catcacccgc aaggtaacaa atcggaagta cccaaaactg aaagaagtgg atgatgtgct
      181 tggtggagca gctgcctggg agaatgttga ctctactgca gagtcgtgtc ccaaatgcga
      241 acatcctcgt gcttacttca tgcagcttca gacccgctct gcagatgagc cgatgaccac
      301 cttctacaag tgctgcaatg ctcagtgtgg acaccgctgg agggattagg gccaggatgg
      361 cccagctgcc ctagtgtgtg cttgccttgt ccctcggggt agatgcttag ctggcagtat
      421 gagttgtgtg tcctgagggt ctttgctagt gtggtggaaa gataaacctt ttgaggtgaa
      481 gagccagggg gtcaggaaat atggcctatc tgccaggcag ggtggatgaa gtcatgaatg
      541 tctgggagtt tttctgtgtg gggaggagac agagacccat aactaaatat gctctgtgta
      601 aagtcctatt ctttcatctt ccactttatt ggcagttgac attcccttac tcccaatcaa
      661 cactcttaaa tatttgtact gtttgtaaaa cttagtacat gtccctaaat atttaactgt
      721 tacttgtaaa cttgtgtaat ttattattta ttttaatcaa aattctgaat atttcattta
      781 aatgaaagtt ggaatattgc cttctcagtg ttttaaatag cttaagaggt agaaagcaaa
      841 ccccaaatca cagtgatccc agaatagact acatacttac atttgaacag gctacgaaaa
      901 tgttaatcac cgggatttca atttctttct ttattcctat tcttactccc ttattcattt
      961 ttttgaacct cccctgattt gcttcctcgg gttgctttaa atgtataaga tactatttat
     1021 ttatttattt attttattta tttatttatt tatttaagac agagtcttgt tctgtcgccc
     1081 aggctggagt gcagtggtac gaattcagct cactgcaacc tctgccttcc gggttcaagg
     1141 gattctcctg tctcagcctc ccaagcagct gggactacag gcatgcacca ccacacctgg
     1201 gtaattttgc atttttagta gagatggggt ttctccatgt tggtcgggct ggtctccaac
     1261 tcctgacctc agataatctg tctgcctcgg cctcctaaag tgctgggatt acaggcgtga
     1321 gccactgtgt cccaccaatc tttacattta atatgactat tttcttattg tt
//
