LOCUS       NM_001018050            4468 bp    mRNA    linear   PRI 13-APR-2024
DEFINITION  Homo sapiens RNA polymerase III subunit H (POLR3H), transcript
            variant 3, mRNA.
ACCESSION   NM_001018050
VERSION     NM_001018050.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Hou,H., Li,Y., Wang,M., Liu,A., Yu,Z., Chen,K., Zhao,D. and Xu,Y.
  TITLE     Structural insights into RNA polymerase III-mediated transcription
            termination through trapping poly-deoxythymidine
  JOURNAL   Nat Commun 12 (1), 6135 (2021)
   PUBMED   34675218
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Ramsay,E.P., Abascal-Palacios,G., Daiss,J.L., King,H., Gouge,J.,
            Pilsl,M., Beuron,F., Morris,E., Gunkel,P., Engel,C. and Vannini,A.
  TITLE     Structure of human RNA polymerase III
  JOURNAL   Nat Commun 11 (1), 6409 (2020)
   PUBMED   33335104
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4468)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 4468)
  AUTHORS   Franca,M.M., Han,X., Funari,M.F.A., Lerario,A.M., Nishi,M.Y.,
            Fontenele,E.G.P., Domenice,S., Jorge,A.A.L., Garcia-Galiano,D.,
            Elias,C.F. and Mendonca,B.B.
  TITLE     Exome Sequencing Reveals the POLR3H Gene as a Novel Cause of
            Primary Ovarian Insufficiency
  JOURNAL   J Clin Endocrinol Metab 104 (7), 2827-2841 (2019)
   PUBMED   30830215
  REMARK    GeneRIF: A pathogenic homozygous missense mutation (c.149A>G;
            p.Asp50Gly) in the POLR3H gene in two unrelated families with
            primary ovarian insufficiency was identified.
REFERENCE   5  (bases 1 to 4468)
  AUTHORS   Owen,T.J., O'Neil,J.D., Dawson,C.W., Hu,C., Chen,X., Yao,Y.,
            Wood,V.H., Mitchell,L.E., White,R.J., Young,L.S. and Arrand,J.R.
  TITLE     Epstein-Barr virus-encoded EBNA1 enhances RNA polymerase
            III-dependent EBER expression through induction of EBER-associated
            cellular transcription factors
  JOURNAL   Mol Cancer 9, 241 (2010)
   PUBMED   20843307
  REMARK    GeneRIF: Findings reveal that RNA polymerase III-dependent EBER
            expression through induction of cellular transcription factors and
            add to the repertoire of EBNA1's transcription-regulatory
            properties.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4468)
  AUTHORS   Greco-Stewart,V.S., Schissel,E. and Pelchat,M.
  TITLE     The hepatitis delta virus RNA genome interacts with the human RNA
            polymerases I and III
  JOURNAL   Virology 386 (1), 12-15 (2009)
   PUBMED   19246067
  REMARK    GeneRIF: The authors demonstrate the interaction of both RNA
            polymerase I and III with hepatitis delta virus RNA, both in vitro
            and in human cells.
REFERENCE   7  (bases 1 to 4468)
  AUTHORS   Wang,A.G., Yoon,S.Y., Oh,J.H., Jeon,Y.J., Kim,M., Kim,J.M.,
            Byun,S.S., Yang,J.O., Kim,J.H., Kim,D.G., Yeom,Y.I., Yoo,H.S.,
            Kim,Y.S. and Kim,N.S.
  TITLE     Identification of intrahepatic cholangiocarcinoma related genes by
            comparison with normal liver tissues using expressed sequence tags
  JOURNAL   Biochem Biophys Res Commun 345 (3), 1022-1032 (2006)
   PUBMED   16712791
REFERENCE   8  (bases 1 to 4468)
  AUTHORS   Hu,P., Wu,S., Sun,Y., Yuan,C.C., Kobayashi,R., Myers,M.P. and
            Hernandez,N.
  TITLE     Characterization of human RNA polymerase III identifies orthologues
            for Saccharomyces cerevisiae RNA polymerase III subunits
  JOURNAL   Mol Cell Biol 22 (22), 8044-8055 (2002)
   PUBMED   12391170
REFERENCE   9  (bases 1 to 4468)
  AUTHORS   Jang,K.L., Collins,M.K. and Latchman,D.S.
  TITLE     The human immunodeficiency virus tat protein increases the
            transcription of human Alu repeated sequences by increasing the
            activity of the cellular transcription factor TFIIIC
  JOURNAL   J Acquir Immune Defic Syndr (1988) 5 (11), 1142-1147 (1992)
   PUBMED   1403646
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA449644.1, DA238361.1, AB051452.1, AK000365.1, AL023553.5 and
            BM701572.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001018050.3.
            
            Transcript Variant: This variant (3) differs in the 5' UTR,
            compared to variant 1. Variants 1, 3, 5, and 6 encode the same
            isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.243919.1,
                                           SRR1803616.103110.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000355209.9/ ENSP00000347345.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                DA449644.1         20-53
            35-444              DA238361.1         67-476
            445-2626            AB051452.1         319-2500
            2627-3926           AK000365.1         214-1513
            3927-3930           AL023553.5         29206-29209         c
            3931-4468           BM701572.1         1-538
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.2"
     gene            1..4468
                     /gene="POLR3H"
                     /gene_synonym="C25; RPC22.9; RPC8"
                     /note="RNA polymerase III subunit H"
                     /db_xref="GeneID:171568"
                     /db_xref="HGNC:HGNC:30349"
                     /db_xref="MIM:619801"
     exon            1..480
                     /gene="POLR3H"
                     /gene_synonym="C25; RPC22.9; RPC8"
                     /inference="alignment:Splign:2.1.0"
     CDS             370..984
                     /gene="POLR3H"
                     /gene_synonym="C25; RPC22.9; RPC8"
                     /EC_number="2.7.7.6"
                     /note="isoform a is encoded by transcript variant 3;
                     DNA-directed RNA polymerase III subunit RPC8; polymerase
                     (RNA) III subunit H; RNA nucleotidyltransferase
                     (DNA-directed); RNA polymerase III subunit C8;
                     DNA-directed RNA polymerase III subunit H; DNA-directed
                     RNA polymerase III subunit 22.9 kDa polypeptide;
                     polymerase (RNA) III (DNA directed) polypeptide H
                     (22.9kD)"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase III subunit RPC8
                     isoform a"
                     /protein_id="NP_001018060.1"
                     /db_xref="CCDS:CCDS14018.1"
                     /db_xref="GeneID:171568"
                     /db_xref="HGNC:HGNC:30349"
                     /db_xref="MIM:619801"
                     /translation="MFVLVEMVDTVRIPPWQFERKLNDSIAEELNKKLANKVVYNVGL
                     CICLFDITKLEDAYVFPGDGASHTKVHFRCVVFHPFLDEILIGKIKGCSPEGVHVSLG
                     FFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDT
                     SPTGPSSADATTSSEELPKKEAPYTLVGSISEPGLGLLSWWTSN"
     exon            481..577
                     /gene="POLR3H"
                     /gene_synonym="C25; RPC22.9; RPC8"
                     /inference="alignment:Splign:2.1.0"
     exon            578..664
                     /gene="POLR3H"
                     /gene_synonym="C25; RPC22.9; RPC8"
                     /inference="alignment:Splign:2.1.0"
     exon            665..728
                     /gene="POLR3H"
                     /gene_synonym="C25; RPC22.9; RPC8"
                     /inference="alignment:Splign:2.1.0"
     exon            729..930
                     /gene="POLR3H"
                     /gene_synonym="C25; RPC22.9; RPC8"
                     /inference="alignment:Splign:2.1.0"
     exon            931..4468
                     /gene="POLR3H"
                     /gene_synonym="C25; RPC22.9; RPC8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1406..1411
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR3H"
                     /gene_synonym="C25; RPC22.9; RPC8"
                     /note="hexamer: AATAAA"
     regulatory      1410..1415
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR3H"
                     /gene_synonym="C25; RPC22.9; RPC8"
                     /note="hexamer: AATACA"
     polyA_site      1425
                     /gene="POLR3H"
                     /gene_synonym="C25; RPC22.9; RPC8"
                     /note="major polyA site"
     polyA_site      1434
                     /gene="POLR3H"
                     /gene_synonym="C25; RPC22.9; RPC8"
     regulatory      4424..4429
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR3H"
                     /gene_synonym="C25; RPC22.9; RPC8"
                     /note="hexamer: AATAAA"
     polyA_site      4468
                     /gene="POLR3H"
                     /gene_synonym="C25; RPC22.9; RPC8"
ORIGIN      
        1 agtcgcgcaa gcgcgagcgg ctgtgcgcgg tgcgtggtgc gtggtgcgtg atacgagcgg
       61 cggcacgtgg cgcgggtgcg gggcgtggag tggcgtggcg tggagtggcg tggcgtggcg
      121 gggtctcgcg gcgcgggcgc gcacccggag ctgtggacgg agagtgcctc cctctggcct
      181 cagtttcctc atgttgtagt agcggagcat ggcccggacc gggcccccga gaccgccccg
      241 tgcaacctca ccgccagcct gggggcctca gcgactggga cgggaccaag gggctcgggg
      301 attctccctg cccccggccc tggtgcgtga ctgaccctcc tgttcccaga gcccccagcg
      361 caggccggga tgttcgtcct ggtggaaatg gtggacaccg tccggatccc cccttggcag
      421 tttgagagga agctcaacga ctccattgcc gaggagctga acaagaagtt ggccaacaag
      481 gtcgtgtaca acgtgggact ctgcatttgt ctgtttgata tcaccaaact ggaggatgcc
      541 tatgtattcc ctggggatgg cgcatcacac accaaagtcc attttcgctg cgtggtgttt
      601 catccattcc tagatgagat tctcattggg aagatcaaag gctgcagccc agaaggagtg
      661 cacgtctctc taggcttctt cgatgacatt ctcatccccc cagagtcact gcagcagcca
      721 gccaagttcg acgaagcgga gcaggtgtgg gtgtgggagt acgagacgga ggaaggagca
      781 cacgacctct acatggacac cggcgaggag atccgcttcc gggtggtgga cgagagcttt
      841 gttgacacgt cccccacagg gcccagctca gcagatgcca ccacttccag tgaggagctg
      901 ccaaagaagg aggctccgta cacgcttgtg ggatccatca gtgagccagg cctgggcctt
      961 ctctcctggt ggaccagcaa ctagccctgg ggctggacag tggaccctac cagcctgcgg
     1021 gaaggtggta tggccggctg tgaagacaac agcagctgag gccgatgcta aggagatagt
     1081 gtctcgagct ggacagtgag gcaaggccag gagtgcccac cagcttgccc cgctcctcca
     1141 ccatctaggc cctgctcctc cttcactgca gtgatggatc catctgtggc tgtggctgtt
     1201 gggcctccac tgaccttgac tgaaaagacc cttcctggac cctggacttg acaacaaacg
     1261 gcacagacac caatcactca cagaacagtc actggttttg gattcttgtt ttccttgcaa
     1321 gaacaaaagg ttctctagaa tccttcagcc accaatgaaa tgatcaggca ggagaaaaac
     1381 tttagatggg agtcttgtca tcaaaaataa atacagttgc atggagctgc ttaagatctg
     1441 aaccaaactc aaaataggaa gacgggggct aaaaagttat tttaagaacc cccccaccac
     1501 aaccacagtc actccgtggg ctaagcgggg agcaggaagc atgtctggtc acaccatctt
     1561 ggaataggaa gccatggctg gacggatcgg atccactgat ggcacacgtg gagctgaact
     1621 tgacgccagc ggcggggcgg ggaggcactg ccctcactgt tgcagttcct tcattctgtt
     1681 gagggcactg ccagcgcgga accactcaat ctgcgtctcg ttgaaggtgt ggttcaggag
     1741 gatggtctcc tgggtcccgt tggggtgctt gatgatgcac ttcaggggct gcaaggagaa
     1801 ggtgggtgtg ggtggaagtg gtgggtactg tgtgcccttg gccccgcagg ggggtcaggg
     1861 agacagggca ctgcctgggg acctaagagg tcagcaggca aaccaaatcc taggccagtc
     1921 tgtgcctgat ggcccaggtc agacccaggt ggcacctaca ggggattcct ggggcccaga
     1981 gggtcctgca gtgggtgggc tgtcccctga gtgccaggtc ctggtgagta aagggaggtt
     2041 ggggaggtgt gagggccccg ggttgactcc aacccccaac tctgagaagc cactttgctt
     2101 tctctgtggg gccacctgga gaactggggg cctttccagc tggaatgaag cctcctggga
     2161 gaagctaccc tggggccagt gcaggagggg aaacaaggtg gctgcccctc accccaccac
     2221 ctcaggggga cccgggcccc taaccttgcc aggggtgaag tccttcaggc cctgaatggt
     2281 cagcttgtcc acagggtgaa tcttgttgta gtcagccggg tcagcgaagg tcagaggcag
     2341 caggccctgt ttcttcaggt tggtctctgg aaagcaggcg gcattcggtc atcgggggcc
     2401 tgactagcct ggagagcttc attttctgaa cccatctggg gctctgggag atgcccctaa
     2461 tcctgctgcc tgggacaatg cccgggcagt gactatgggg ccctggtctg ctccctttca
     2521 ctaggggtgc ccctagcaag tgtgcgctgg tgctgaggtc tgcgaggcct tcctgctgca
     2581 cattcatgat cagaaagaac cccctggcac atgtcctaga tctcagcggc tcagatctac
     2641 aagcctggaa gcagggcctg cgggaagccg ggaagctgag cgtcggatgt gcactgtgtg
     2701 agcctaagaa ggggcaagag gctgacgggc ctgtgggctg cagtggacac agcctagaac
     2761 caggcccaac tcagccacgg gcagggagag tgaccttgat cactagggat gggatgagga
     2821 tggcctgggt acagactcca gctcaccgtg gatcctggca aagctcttgg tgatgatggc
     2881 ccggccccca aggtggcgag gctccagagc tgcatgctcc cggctcgagc cctcgccgta
     2941 gttctcgtct ccgatcacca cccacctgat gccatgtttc tgtcaggcaa gcgggggtaa
     3001 ggttataagg cagaggaggg caccgtcctc acctgtctgg ccctacctgc ctggcctcac
     3061 ccacctggcg cctgggcatc ctgaggctcc cggtaaaggg gctggagggg cccttcctcg
     3121 cctgctgcct aagggcaagt gaagcctgcg tgaatgagac ccaaacgagg ccccgagggg
     3181 acagaggcac gtggttgttt gcagtggcaa agaagacaga agccacccat tggtgcttgg
     3241 cagaggcagg tgtgtgtgga gggaggttgg gtgagacagg gacggcctcc gggccccaca
     3301 caagatgcat ctgtgaagct caaggcgccc ccttcctggg agcaccctct gccccaccca
     3361 gccaccaggg ggtgccagag tcctttcacc cggctgggcc aggacttggg ttcagccctg
     3421 gttctgtttg tttctcaaag cagaaaaagt ccaatcacct cagaccctct ctgcctgctc
     3481 actgtgaggc cccgtgtagc cccctctctg agcttttggc ctctgacttc ctgagtcggc
     3541 ccccttcctt gtctttgtct agggccagat atcctaagat atctctcact aactcctcct
     3601 ggtgcaggga cccggctgta ccaccggacc tgggcctcat gccttctcag ccacagggag
     3661 gggctgcagg cctctcctcc cttccacagc agtccccttt gggcttggtc ggcctccttc
     3721 ccagtttcca ctcccctaat gtcctccctg cacctgcctt gcaggcccct tcaggagtga
     3781 ccactggcat tgcccctatc aactctgacc caccttgtag tagcgggcag tgtcagggac
     3841 ggggccaaac tcctgagtga cggcattgcg cacggagttg gccttgccgt tttcaatgtt
     3901 gatggcacca atgagcaggt tgttggagat gttatccaag tgcccacgga acttgagcca
     3961 ggggccagca gctgagatgt ggtcagtggt acacttccct ttgaccttgg gtggtaagta
     4021 gagaggacag aggtcagggc atggccctgg aggggtggat gacaagccct ccagaggcag
     4081 gcagcaggcc ccggggccca ggaggggtga cagaggggtg agaggcaggc aagtcttcag
     4141 acacgtgttc tgtggtcctg gtgcttccac cctaaggcca catgggctca ggccaggcct
     4201 tcatcccctc aaaggcctct ggctgctcag tctcccttat ggggcagagg ccgcccaaag
     4261 ttgggacagg tcaatgttcg ctcacccttc aggcctcagc tgcaagatca gccacagacc
     4321 tcatgctgga cacaggagcc caggacatgc ctgggtcctg gctgatgact gattagtctc
     4381 ttctgtcaca ggggtgcctg gagacccctt aataagcagc ataaataaat atctgacaca
     4441 gattggaggc ctctggccac accaaaaa
//
