LOCUS       NM_032305               1178 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens RNA polymerase III subunit GL (POLR3GL), transcript
            variant 1, mRNA.
ACCESSION   NM_032305
VERSION     NM_032305.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1178)
  AUTHORS   Wang,Q., Li,S., Wan,F., Xu,Y., Wu,Z., Cao,M., Lan,P., Lei,M. and
            Wu,J.
  TITLE     Structural insights into transcriptional regulation of human RNA
            polymerase III
  JOURNAL   Nat Struct Mol Biol 28 (2), 220-227 (2021)
   PUBMED   33558766
  REMARK    GeneRIF: Structural insights into transcriptional regulation of
            human RNA polymerase III.
REFERENCE   4  (bases 1 to 1178)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1178)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1178)
  AUTHORS   Petrie,J.L., Swan,C., Ingram,R.M., Frame,F.M., Collins,A.T.,
            Dumay-Odelot,H., Teichmann,M., Maitland,N.J. and White,R.J.
  TITLE     Effects on prostate cancer cells of targeting RNA polymerase III
  JOURNAL   Nucleic Acids Res 47 (8), 3937-3956 (2019)
   PUBMED   30820548
  REMARK    GeneRIF: Depletion of POLR3G selectively triggers proliferative
            arrest and differentiation of prostate cancer cells, responses not
            elicited when POLR3GL is depleted.
REFERENCE   7  (bases 1 to 1178)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   8  (bases 1 to 1178)
  AUTHORS   Renaud,M., Praz,V., Vieu,E., Florens,L., Washburn,M.P., l'Hote,P.
            and Hernandez,N.
  TITLE     Gene duplication and neofunctionalization: POLR3G and POLR3GL
  JOURNAL   Genome Res 24 (1), 37-51 (2014)
   PUBMED   24107381
REFERENCE   9  (bases 1 to 1178)
  AUTHORS   Lefevre,S., Dumay-Odelot,H., El-Ayoubi,L., Budd,A., Legrand,P.,
            Pinaud,N., Teichmann,M. and Fribourg,S.
  TITLE     Structure-function analysis of hRPC62 provides insights into RNA
            polymerase III transcription initiation
  JOURNAL   Nat Struct Mol Biol 18 (3), 352-358 (2011)
   PUBMED   21358628
  REMARK    Erratum:[Nat Struct Mol Biol. 2011 Apr;18(4):516]
REFERENCE   10 (bases 1 to 1178)
  AUTHORS   Haurie,V., Durrieu-Gaillard,S., Dumay-Odelot,H., Da Silva,D.,
            Rey,C., Prochazkova,M., Roeder,R.G., Besser,D. and Teichmann,M.
  TITLE     Two isoforms of human RNA polymerase III with specific functions in
            cell growth and transformation
  JOURNAL   Proc Natl Acad Sci U S A 107 (9), 4176-4181 (2010)
   PUBMED   20154270
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BI914801.1 and BC004355.1.
            
            On Nov 23, 2018 this sequence version replaced NM_032305.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.635323.1,
                                           SRR5189667.245848.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369314.2/ ENSP00000358320.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                BI914801.1         9-21
            14-1178             BC004355.1         1-1165
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q21.1"
     gene            1..1178
                     /gene="POLR3GL"
                     /gene_synonym="flj32422; RPC32HOM; SOFM"
                     /note="RNA polymerase III subunit GL"
                     /db_xref="GeneID:84265"
                     /db_xref="HGNC:HGNC:28466"
                     /db_xref="MIM:617457"
     exon            1..79
                     /gene="POLR3GL"
                     /gene_synonym="flj32422; RPC32HOM; SOFM"
                     /inference="alignment:Splign:2.1.0"
     exon            80..246
                     /gene="POLR3GL"
                     /gene_synonym="flj32422; RPC32HOM; SOFM"
                     /inference="alignment:Splign:2.1.0"
     CDS             121..777
                     /gene="POLR3GL"
                     /gene_synonym="flj32422; RPC32HOM; SOFM"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DNA-directed RNA polymerase III subunit RPC7-like;
                     RPC32-like protein; alternative RNA polymerase III subunit
                     32; RNA polymerase III subunit C7-like; polymerase (RNA)
                     III subunit G like; polymerase (RNA) III (DNA directed)
                     polypeptide G (32kD)-like; DNA-directed RNA polymerase III
                     subunit G-like; RPC32-beta; RNA polymerase III 32 kDa beta
                     subunit; RNA polymerase III subunit G like"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase III subunit
                     RPC7-like isoform 1"
                     /protein_id="NP_115681.1"
                     /db_xref="CCDS:CCDS72875.1"
                     /db_xref="GeneID:84265"
                     /db_xref="HGNC:HGNC:28466"
                     /db_xref="MIM:617457"
                     /translation="MASRGGGRGRGRGQLTFNVEAVGIGKGDALPPPTLQPSPLFPPL
                     EFRPVPLPSGEEGEYVLALKQELRGAMRQLPYFIRPAVPKRDVERYSDKYQMSGPIDN
                     AIDWNPDWRRLPRELKIRVRKLQKERITILLPKRPPKTTEDKEETIQKLETLEKKEEE
                     VTSEEDEEKEEEEEKEEEEEEEYDEEEHEEETDYIMSYFDNGEDFGGDSDDNMDEAIY
                     "
     exon            247..376
                     /gene="POLR3GL"
                     /gene_synonym="flj32422; RPC32HOM; SOFM"
                     /inference="alignment:Splign:2.1.0"
     exon            377..445
                     /gene="POLR3GL"
                     /gene_synonym="flj32422; RPC32HOM; SOFM"
                     /inference="alignment:Splign:2.1.0"
     exon            446..502
                     /gene="POLR3GL"
                     /gene_synonym="flj32422; RPC32HOM; SOFM"
                     /inference="alignment:Splign:2.1.0"
     exon            503..576
                     /gene="POLR3GL"
                     /gene_synonym="flj32422; RPC32HOM; SOFM"
                     /inference="alignment:Splign:2.1.0"
     exon            577..690
                     /gene="POLR3GL"
                     /gene_synonym="flj32422; RPC32HOM; SOFM"
                     /inference="alignment:Splign:2.1.0"
     exon            691..1178
                     /gene="POLR3GL"
                     /gene_synonym="flj32422; RPC32HOM; SOFM"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1158..1163
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR3GL"
                     /gene_synonym="flj32422; RPC32HOM; SOFM"
                     /note="hexamer: AATAAA"
     polyA_site      1178
                     /gene="POLR3GL"
                     /gene_synonym="flj32422; RPC32HOM; SOFM"
                     /note="major polyA site"
ORIGIN      
        1 agaacgccac cgacttgagg aagcccagta catttcaagt tggtcgcggc ttgggctccg
       61 ctttggggag gggcagcagg tttattcact ggatctctga atacccaggc cccctccacc
      121 atggccagcc ggggtggggg ccggggtcgt ggccggggcc agttgacctt caacgtggag
      181 gccgtgggca ttgggaaagg ggatgctttg cccccaccca ccctgcagcc ttctccactc
      241 ttccctccct tggagttccg cccagtacct ttgccctcag gcgaggaagg ggaatatgtc
      301 ctggcactga agcaagagct acgaggagcc atgaggcagc tcccctactt catccggcca
      361 gctgtcccca agagagatgt ggagcgttat tcagacaaat atcagatgtc aggtccgatt
      421 gacaatgcca tcgattggaa ccctgattgg cggcgtctac cccgggagct aaagatccga
      481 gtgcggaagc tacagaagga acggattaca attctgctcc ccaagaggcc ccctaagacc
      541 acagaagata aggaggaaac aatacagaaa ctagagaccc tggagaagaa ggaagaagaa
      601 gtaacttcag aggaggatga ggagaaagaa gaagaagaag agaaggaaga ggaggaagaa
      661 gaagagtatg atgaagaaga acatgaagag gaaactgatt acatcatgtc atattttgac
      721 aatggagagg actttggtgg tgacagtgat gacaatatgg acgaggctat atactgaaga
      781 aggactctgg accctcgtgt ctttctttag gatacagaga gtaactgtac ctattatttg
      841 tttcttcaga caagcaaatc atttggtcag agttcatata atctgtctgt tccctggaga
      901 tgggaataga ggatgatgac agtttatttt ctacacttcc cctccttcca catttgtatc
      961 acctttgcta tcttggggaa agtgcaaagg acaaacatct caattgtatg aagggagaaa
     1021 ggagaattga aagaagaact ggggttgtta gagctgagat gactgtacac atacccctgc
     1081 ccaatttata tagctctttg tggagataat taggggtggg agcagtttga aggagtaagc
     1141 ctggttttat acttttaaat aaagtgtttt tatctgtc
//
