LOCUS       NM_006466               2156 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens RNA polymerase III subunit F (POLR3F), transcript
            variant 1, mRNA.
ACCESSION   NM_006466
VERSION     NM_006466.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Hou,H., Li,Y., Wang,M., Liu,A., Yu,Z., Chen,K., Zhao,D. and Xu,Y.
  TITLE     Structural insights into RNA polymerase III-mediated transcription
            termination through trapping poly-deoxythymidine
  JOURNAL   Nat Commun 12 (1), 6135 (2021)
   PUBMED   34675218
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2156)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2156)
  AUTHORS   Ramsay,E.P., Abascal-Palacios,G., Daiss,J.L., King,H., Gouge,J.,
            Pilsl,M., Beuron,F., Morris,E., Gunkel,P., Engel,C. and Vannini,A.
  TITLE     Structure of human RNA polymerase III
  JOURNAL   Nat Commun 11 (1), 6409 (2020)
   PUBMED   33335104
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2156)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 2156)
  AUTHORS   Hu,P., Wu,S., Sun,Y., Yuan,C.C., Kobayashi,R., Myers,M.P. and
            Hernandez,N.
  TITLE     Characterization of human RNA polymerase III identifies orthologues
            for Saccharomyces cerevisiae RNA polymerase III subunits
  JOURNAL   Mol Cell Biol 22 (22), 8044-8055 (2002)
   PUBMED   12391170
REFERENCE   7  (bases 1 to 2156)
  AUTHORS   Mertens,C., Hofmann,I., Wang,Z., Teichmann,M., Sepehri Chong,S.,
            Schnolzer,M. and Franke,W.W.
  TITLE     Nuclear particles containing RNA polymerase III complexes
            associated with the junctional plaque protein plakophilin 2
  JOURNAL   Proc Natl Acad Sci U S A 98 (14), 7795-7800 (2001)
   PUBMED   11416169
REFERENCE   8  (bases 1 to 2156)
  AUTHORS   Jones,E., Kimura,H., Vigneron,M., Wang,Z., Roeder,R.G. and
            Cook,P.R.
  TITLE     Isolation and characterization of monoclonal antibodies directed
            against subunits of human RNA polymerases I, II, and III
  JOURNAL   Exp Cell Res 254 (1), 163-172 (2000)
   PUBMED   10623476
REFERENCE   9  (bases 1 to 2156)
  AUTHORS   Hsieh,Y.J., Kundu,T.K., Wang,Z., Kovelman,R. and Roeder,R.G.
  TITLE     The TFIIIC90 subunit of TFIIIC interacts with multiple components
            of the RNA polymerase III machinery and contains a histone-specific
            acetyltransferase activity
  JOURNAL   Mol Cell Biol 19 (11), 7697-7704 (1999)
   PUBMED   10523658
REFERENCE   10 (bases 1 to 2156)
  AUTHORS   Wang,Z. and Roeder,R.G.
  TITLE     Three human RNA polymerase III-specific subunits form a subcomplex
            with a selective function in specific transcription initiation
  JOURNAL   Genes Dev 11 (10), 1315-1326 (1997)
   PUBMED   9171375
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121893.21.
            
            On Nov 23, 2018 this sequence version replaced NM_006466.3.
            
            Summary: The protein encoded by this gene is one of more than a
            dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III),
            which transcribes 5S ribosomal RNA and tRNA genes. This protein has
            been shown to bind both TFIIIB90 and TBP, two subunits of RNA
            polymerase III transcription initiation factor IIIB (TFIIIB).
            Unlike most of the other RNA Pol III subunits, the encoded protein
            is unique to this polymerase. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Sep 2013].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.180635.1,
                                           SRR3476690.1022305.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000377603.5/ ENSP00000366828.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-179               AL121893.21        57926-58104
            180-297             AL121893.21        59480-59597
            298-365             AL121893.21        63378-63445
            366-433             AL121893.21        63927-63994
            434-546             AL121893.21        65611-65723
            547-690             AL121893.21        70574-70717
            691-798             AL121893.21        70938-71045
            799-990             AL121893.21        72155-72346
            991-2156            AL121893.21        74017-75182
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p11.23"
     gene            1..2156
                     /gene="POLR3F"
                     /gene_synonym="C34; IMD101; RPC39; RPC6"
                     /note="RNA polymerase III subunit F"
                     /db_xref="GeneID:10621"
                     /db_xref="HGNC:HGNC:15763"
                     /db_xref="MIM:617455"
     exon            1..179
                     /gene="POLR3F"
                     /gene_synonym="C34; IMD101; RPC39; RPC6"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..1068
                     /gene="POLR3F"
                     /gene_synonym="C34; IMD101; RPC39; RPC6"
                     /EC_number="2.7.7.6"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DNA-directed RNA polymerase III subunit RPC6; polymerase
                     (RNA) III (DNA directed) polypeptide F, 39 kDa; RNA
                     polymerase III C39 subunit; RNA polymerase III subunit C6;
                     polymerase (RNA) III subunit F"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase III subunit RPC6
                     isoform 1"
                     /protein_id="NP_006457.2"
                     /db_xref="CCDS:CCDS13135.1"
                     /db_xref="GeneID:10621"
                     /db_xref="HGNC:HGNC:15763"
                     /db_xref="MIM:617455"
                     /translation="MAEVKVKVQPPDADPVEIENRIIELCHQFPHGITDQVIQNEMPH
                     IEAQQRAVAINRLLSMGQLDLLRSNTGLLYRIKDSQNAGKMKGSDNQEKLVYQIIEDA
                     GNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYMLYNLQPDRS
                     VTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPMIQRNSSFASSHEVWK
                     YICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYRAVNPI
                     IPPTGLVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF"
     exon            180..297
                     /gene="POLR3F"
                     /gene_synonym="C34; IMD101; RPC39; RPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            298..365
                     /gene="POLR3F"
                     /gene_synonym="C34; IMD101; RPC39; RPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            366..433
                     /gene="POLR3F"
                     /gene_synonym="C34; IMD101; RPC39; RPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            434..546
                     /gene="POLR3F"
                     /gene_synonym="C34; IMD101; RPC39; RPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            547..690
                     /gene="POLR3F"
                     /gene_synonym="C34; IMD101; RPC39; RPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            691..798
                     /gene="POLR3F"
                     /gene_synonym="C34; IMD101; RPC39; RPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            799..990
                     /gene="POLR3F"
                     /gene_synonym="C34; IMD101; RPC39; RPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            991..2156
                     /gene="POLR3F"
                     /gene_synonym="C34; IMD101; RPC39; RPC6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1301..1306
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR3F"
                     /gene_synonym="C34; IMD101; RPC39; RPC6"
                     /note="hexamer: AATAAA"
     polyA_site      1320
                     /gene="POLR3F"
                     /gene_synonym="C34; IMD101; RPC39; RPC6"
                     /note="major polyA site"
     regulatory      2131..2136
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR3F"
                     /gene_synonym="C34; IMD101; RPC39; RPC6"
                     /note="hexamer: AATAAA"
     polyA_site      2156
                     /gene="POLR3F"
                     /gene_synonym="C34; IMD101; RPC39; RPC6"
                     /note="major polyA site"
ORIGIN      
        1 gaagaaggcc cctcggcctc agcagagcgc tatcctccac tggttccccg ggttccccgg
       61 cttgctaccg ggctgctccg tgcatctttc cccccaggcg tcaggaactg cgccctcatg
      121 gcggaggtga aggtgaaggt gcagccgcct gacgcggatc cggtcgaaat agaaaacagg
      181 attatagaat tatgtcacca gttccctcat ggaatcacag accaagtaat tcagaatgaa
      241 atgcctcata tagaagccca gcagcgggca gtagccatca ataggttgtt gtctatgggt
      301 cagttggatc tcttaaggag caatacgggc cttttatata gaataaagga ctctcagaat
      361 gctggtaaaa tgaagggatc cgataaccaa gaaaaactag tatatcaaat catagaggat
      421 gcaggaaata aaggaatatg gagcagagat atccgctata aaagtaattt gccattaaca
      481 gaaatcaaca aaattctgaa gaatctggaa agtaaaaagc ttatcaaagc tgttaagtct
      541 gtagcagcct caaaaaagaa ggtgtatatg ctctataacc tgcagccaga ccggtctgtg
      601 actggtggag cctggtacag tgaccaggat tttgaatctg aatttgtaga ggtgcttaac
      661 caacagtgtt ttaaattcct acagtccaag gcagaaacag cacgagaaag caaacagaac
      721 ccaatgatac aaagaaatag ttcatttgcc tcatcacatg aagtgtggaa atatatctgc
      781 gaattgggaa tcagtaaggt agagttatcc atggaagaca ttgaaaccat cctgaataca
      841 ctcatttatg atggaaaagt ggagatgacg attattgctg caaaagaagg cacagttggc
      901 agtgtagatg gacacatgaa actgtacagg gcagtcaatc caatcatccc tcccacaggt
      961 ttggtccggg caccctgtgg actctgcccg gtttttgatg actgccacga aggtggtgag
     1021 atttcaccat ctaactgtat ttacatgaca gagtggctcg aattttaata gagagctatg
     1081 aactttattg acattttgca aatgaagtta cttagggagc agataattta attcatgatg
     1141 gaacacgaaa tctccttgaa agcaaacttc acaataatgg acgtagactt gctgctatga
     1201 aaacatattt tttttattta tgaagactaa atttatattg gtaaaatagc cagtagaata
     1261 tgaaagaaat aaggttagta gtgaaattca ttcttcaata aataaaacac tttgaaactc
     1321 cggaggacca catctttcaa gacttctgat gggtcaagga aaaagatgcc aacatacccg
     1381 tatttaccaa gtactatgat aatggctaga gtataaaaat gttcttttta aagttattta
     1441 ttaagttctt cattggaagt ttttttttat atctggttca ctaccaccat tttctgtttc
     1501 ctactttctc agtggtttca ttgaaaagaa attagaaggg gttaaaggca ggaatagcaa
     1561 agagtgcaaa cttggggtat gactggggga gagtggaaca tgccttttcc gcacaatatt
     1621 aattcctttt tgtatcagaa atgttctttt aggagattat gctaccatac ttacttcaaa
     1681 cccaatgact actgtcaagg acatattttc agtacataaa tactatcatt ttcattctaa
     1741 agaatatttt cactgttcct tctttcttaa agtcttatgt ttcactcttt aactcaaatg
     1801 tattctttgt tagaatttac cctagattct tatttaatgt ctgcagtaga ctgaatgttt
     1861 gtgtgccccc agaattctaa tgttgaaatc tcatttccaa tgtgatggta tttggaggtg
     1921 gggcttttgg taagtgatag gtcaggagag taacagcgct catgaatggg attagtgccc
     1981 ttatataaag agacccagag agctccatca ccccttctgc catgtgaaag ggagaagaca
     2041 aacatccacg aaccaggaag tgggtcctca ccagaaaaca aatctgtaag caccttgatc
     2101 ttggacttct cagcctccag aattgtgaga aataaatttc tgttgttgat aaacca
//
