LOCUS       NM_018119               3690 bp    mRNA    linear   PRI 13-APR-2024
DEFINITION  Homo sapiens RNA polymerase III subunit E (POLR3E), transcript
            variant 1, mRNA.
ACCESSION   NM_018119
VERSION     NM_018119.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Hou,H., Li,Y., Wang,M., Liu,A., Yu,Z., Chen,K., Zhao,D. and Xu,Y.
  TITLE     Structural insights into RNA polymerase III-mediated transcription
            termination through trapping poly-deoxythymidine
  JOURNAL   Nat Commun 12 (1), 6135 (2021)
   PUBMED   34675218
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Ramsay,E.P., Abascal-Palacios,G., Daiss,J.L., King,H., Gouge,J.,
            Pilsl,M., Beuron,F., Morris,E., Gunkel,P., Engel,C. and Vannini,A.
  TITLE     Structure of human RNA polymerase III
  JOURNAL   Nat Commun 11 (1), 6409 (2020)
   PUBMED   33335104
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3690)
  AUTHORS   Ramanathan,A., Weintraub,M., Orlovetskie,N., Serruya,R., Mani,D.,
            Marcu,O., Stepensky,P., Weisblum,Y., Djian,E., Shaag,A.,
            Revel-Vilk,S., Fried,I., Kotler,M., Rouvinski,A., Wolf,D.,
            Elpeleg,O. and Jarrous,N.
  TITLE     A mutation in POLR3E impairs antiviral immune response and RNA
            polymerase III
  JOURNAL   Proc Natl Acad Sci U S A 117 (36), 22113-22121 (2020)
   PUBMED   32843346
  REMARK    GeneRIF: A mutation in POLR3E impairs antiviral immune response and
            RNA polymerase III.
REFERENCE   4  (bases 1 to 3690)
  AUTHORS   Carter-Timofte,M.E., Hansen,A.F., Christiansen,M., Paludan,S.R. and
            Mogensen,T.H.
  TITLE     Mutations in RNA Polymerase III genes and defective DNA sensing in
            adults with varicella-zoster virus CNS infection
  JOURNAL   Genes Immun 20 (3), 214-223 (2019)
   PUBMED   29728610
  REMARK    GeneRIF: Mutations in the coding region of POLR3E cause impaired
            expression of antiviral cytokines and susceptibility to
            varicella-zoster virus CNS infection.
REFERENCE   5  (bases 1 to 3690)
  AUTHORS   Renaud,M., Praz,V., Vieu,E., Florens,L., Washburn,M.P., l'Hote,P.
            and Hernandez,N.
  TITLE     Gene duplication and neofunctionalization: POLR3G and POLR3GL
  JOURNAL   Genome Res 24 (1), 37-51 (2014)
   PUBMED   24107381
REFERENCE   6  (bases 1 to 3690)
  AUTHORS   Hu,P., Wu,S., Sun,Y., Yuan,C.C., Kobayashi,R., Myers,M.P. and
            Hernandez,N.
  TITLE     Characterization of human RNA polymerase III identifies orthologues
            for Saccharomyces cerevisiae RNA polymerase III subunits
  JOURNAL   Mol Cell Biol 22 (22), 8044-8055 (2002)
   PUBMED   12391170
  REMARK    GeneRIF: Data describe the purification and identification of RNA
            polymerase III subunits RPC2 and RPC5.
REFERENCE   7  (bases 1 to 3690)
  AUTHORS   Jang,K.L., Collins,M.K. and Latchman,D.S.
  TITLE     The human immunodeficiency virus tat protein increases the
            transcription of human Alu repeated sequences by increasing the
            activity of the cellular transcription factor TFIIIC
  JOURNAL   J Acquir Immune Defic Syndr (1988) 5 (11), 1142-1147 (1992)
   PUBMED   1403646
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK026645.1, AK001371.1, AK222671.1, AA282721.1, AC092338.4 and
            AW205673.1.
            
            On Nov 22, 2018 this sequence version replaced NM_018119.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK026645.1, SRR1803614.117547.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299853.10/ ENSP00000299853.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1112              AK026645.1         1-1112
            1113-2574           AK001371.1         1088-2549
            2575-3038           AK222671.1         1484-1947
            3039-3175           AA282721.1         29-165
            3176-3176           AC092338.4         51799-51799
            3177-3357           AA282721.1         167-347
            3358-3690           AW205673.1         17-349              c
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p12.2"
     gene            1..3690
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /note="RNA polymerase III subunit E"
                     /db_xref="GeneID:55718"
                     /db_xref="HGNC:HGNC:30347"
                     /db_xref="MIM:617815"
     exon            1..129
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            130..203
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     CDS             168..2294
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /note="isoform 1 is encoded by transcript variant 1; RNA
                     polymerase III 80 kDa subunit RPC5; DNA-directed RNA
                     polymerase III subunit RPC5; RNA polymerase III subunit
                     C5; DNA-directed RNA polymerase III 80 kDa polypeptide;
                     polymerase (RNA) III (DNA directed) polypeptide E (80kD);
                     polymerase (RNA) III subunit E"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase III subunit RPC5
                     isoform 1"
                     /protein_id="NP_060589.1"
                     /db_xref="CCDS:CCDS10605.1"
                     /db_xref="GeneID:55718"
                     /db_xref="HGNC:HGNC:30347"
                     /db_xref="MIM:617815"
                     /translation="MANEEDDPVVQEIDVYLAKSLAEKLYLFQYPVRPASMTYDDIPH
                     LSAKIKPKQQKVELEMAIDTLNPNYCRSKGEQIALNVDGACADETSTYSSKLMDKQTF
                     CSSQTTSNTSRYAAALYRQGELHLTPLHGILQLRPSFSYLDKADAKHREREAANEAGD
                     SSQDEAEDDVKQITVRFSRPESEQARQRRVQSYEFLQKKHAEEPWVHLHYYGLRDSRS
                     EHERQYLLCPGSSGVENTELVKSPSEYLMMLMPPSQEEEKDKPVAPSNVLSMAQLRTL
                     PLADQIKILMKNVKVMPFANLMSLLGPSIDSVAVLRGIQKVAMLVQGNWVVKSDILYP
                     KDSSSPHSGVPAEVLCRGRDFVMWKFTQSRWVVRKEVATVTKLCAEDVKDFLEHMAVV
                     RINKGWEFILPYDGEFIKKHPDVVQRQHMLWTGIQAKLEKVYNLVKETMPKKPDAQSG
                     PAGLVCGDQRIQVAKTKAQQNHALLERELQRRKEQLRVPAVPPGVRIKEEPVSEEGEE
                     DEEQEAEEEPMDTSPSGLHSKLANGLPLGRAAGTDSFNGHPPQGCASTPVARELKAFV
                     EATFQRQFVLTLSELKRLFNLHLASLPPGHTLFSGISDRMLQDTVLAAGCKQILVPFP
                     PQTAASPDEQKVFALWESGDMSDQHRQVLLEIFSKNYRVRRNMIQSRLTQECGEDLSK
                     QEVDKVLKDCCVSYGGMWYLKGTVQS"
     exon            204..254
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            255..332
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            333..448
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            449..531
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            532..639
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            640..689
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            690..809
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            810..895
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            896..940
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1032
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1153
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1235
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1295
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1453
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1515
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..2033
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2034..2111
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2112..2237
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2238..3690
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2579..2584
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /note="hexamer: AATAAA"
     polyA_site      2619
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /note="major polyA site"
     regulatory      2775..2780
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /note="hexamer: ATTAAA"
     polyA_site      2798
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /note="major polyA site"
     regulatory      3014..3019
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /note="hexamer: AATAAA"
     polyA_site      3038
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
     regulatory      3671..3676
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
                     /note="hexamer: AGTAAA"
     polyA_site      3690
                     /gene="POLR3E"
                     /gene_synonym="C37; RPC5; SIN"
ORIGIN      
        1 acgtgtccgc cggagtttct ccaccagcaa catggccgcc gcctgagagg agagccgggc
       61 cgccgccgtc tctgcagccc gcgggtaact gggccgttgc cgccgtccgc gctcggcccc
      121 cgcggagaga tcgagctgaa ggactgcgcg gctggctctc ctctagtatg gccaatgaag
      181 aggatgaccc agttgtacag gagatcgatg tgtacttggc caagagtctg gcggaaaagc
      241 tgtatctatt tcagtaccct gtgcgtccag cctcgatgac ctacgatgac attccgcacc
      301 tctcagccaa gatcaagccc aagcagcaga aggtagagct tgagatggcc atcgacaccc
      361 tgaaccccaa ctattgccgc agcaaagggg agcagattgc gctgaacgtg gacggggcct
      421 gcgccgacga gaccagcacg tattcctcga agctgatgga caagcagacc ttctgctctt
      481 cccagaccac cagtaacaca tcccgttatg ccgctgcact ctacaggcaa ggtgagctcc
      541 acctgacacc tttacatggc atcctgcagc tgcggcccag cttctcctac ctggataagg
      601 ctgacgccaa gcaccgggag agggaggcgg ccaacgaggc aggggactct tcacaggatg
      661 aggcggaaga cgatgttaag cagatcacgg tgcggttctc ccggccggag tcagagcagg
      721 cccgccagcg ccgtgtgcag tcctatgagt tcctgcagaa gaagcacgca gaggagccct
      781 gggtccacct gcattactat ggcctgaggg acagtcgctc tgagcatgag cgtcagtacc
      841 tgctgtgccc cggctcaagc ggggtggaga acacggagct cgtcaagtca cccagtgagt
      901 acctgatgat gctgatgcca cccagccagg aggaggagaa agacaagcct gtggccccca
      961 gcaacgtcct gtcgatggcc cagctgcgca cgctgcccct ggccgatcag atcaagatcc
     1021 tgatgaagaa tgtgaaggtc atgccttttg ccaacttgat gagcctcctg ggcccctcca
     1081 tcgattccgt ggctgttctg cggggcatcc agaaggtggc gatgttggtc caagggaact
     1141 gggtggtgaa gagtgacatc ctatacccca aggactcgtc cagccctcac agcggcgtgc
     1201 ctgctgaggt gctctgcagg ggccgagact tcgttatgtg gaagttcacg cagagccgct
     1261 gggtggttag gaaagaggtg gcaaccgtga ccaaactctg cgccgaggat gtgaaggact
     1321 tcctggagca catggccgtg gtgaggatca acaaaggctg ggagttcatt ctgccttatg
     1381 atggggagtt catcaagaag cacccggatg tggtccagcg gcagcacatg ctgtggacgg
     1441 gtatccaggc caaactggaa aaagtctata atcttgtaaa ggaaaccatg ccaaagaagc
     1501 cggatgcaca atcagggcct gccgggctgg tctgtgggga ccagcggatc caagtagcca
     1561 aaaccaaggc ccagcagaac cacgcgttgc tggagcggga gctgcagcgg cggaaggagc
     1621 agctgcgggt gcctgcggtc ccgcccggtg tgcggatcaa ggaggagccc gtgagcgagg
     1681 agggcgagga ggacgaggag caggaggcgg aggaggagcc catggacact tcccccagcg
     1741 gcctccacag caagctggcc aacgggctgc ctctcgggcg ggctgcgggc acagacagct
     1801 tcaacgggca cccgccccag ggctgcgcca gcacccctgt ggctcgggaa ctgaaggcct
     1861 tcgtggaggc cacctttcag agacagtttg tgctcacgct gagcgaactc aagcgcctct
     1921 tcaatctgca cttggccagc ctgccccccg gccacacact cttcagcggc atctcggacc
     1981 gcatgctaca ggacacggtg ctggccgccg gttgcaagca gatactggtg ccttttcccc
     2041 cccagactgc tgcttccccg gatgagcaga aggtgtttgc cctctgggag tctggagaca
     2101 tgagtgatca gcatcgacag gttttgcttg aaattttttc caaaaattac cgggtacgcc
     2161 gaaacatgat ccagtctcgg ttgactcaag agtgtggaga agatctcagt aaacaggagg
     2221 tggataaagt actaaaggac tgctgtgtaa gctatggtgg catgtggtac cttaaaggga
     2281 cagtacagtc ttgacaatag tagcaaacta ctaacccagc aaatctaagc ccaaggaaga
     2341 agggcggaac cagaagtagg gcctcgactt gcttcagacg acacagagca agaggaactg
     2401 accatctcat gacctgtggc attgcacggt gcagtggaca gaagggatta tcctcagcca
     2461 gtcgcagggt cagcttaagt tagttagatc actcccagaa gagaccagct gggaccttct
     2521 ttgcagtaca atttgaaatt cctgatgtat tttgcttatt atttggtttc attctcataa
     2581 taaagagagt gtatactgac atgggcagga tgataaaaat catggtttaa tattttcttt
     2641 tgtaaactta atgccaacaa ggtctaagtt atgtttacaa catgaagaaa acctcaaagt
     2701 tcttaatttt taaaatgcct agaagacaat atttagtctt ggattatcta tctgctaaga
     2761 cctccaccaa tttcattaaa ccaaattgaa ttattctatt cttgggattc tgtggccact
     2821 tcacctttga caacaaccta ctttatgtag cagtctcaac tgtttacatg aaccatagca
     2881 aaaaaatcag aatcaaatcc atctcctttt aatgtttgca gaaagatgca aacaaaacca
     2941 ggtaagtatg gaacaatgtg taagtgaggt tatcacactt tgatgtaaaa atttctattt
     3001 tgtgtatttt taaaataaat gcaaacacta aactagcatc atggtgctat cttcaaatgg
     3061 ttacaggaag gcgatcttgt tgggctcagc gttacgtgta tctagaggga acggagctaa
     3121 tggaggaaga cagagcagca ctgtccacta gaaagaggcg agtcacgtac ataattttga
     3181 aagtttctag caaccacatt agtttaaaaa ggtcatgtta gcatattatt tagctcaaaa
     3241 tatctaaaac atcaacataa ggtaaaagtc ttcaaaatct ggcattttac actttcctca
     3301 acactcagtt tggactagcc acatcataag tgctccgatg ccacatgggg ctatttgcga
     3361 cagtattgga tagcacagcc ctagaaactg atggaaaaaa taagagtaag ctaggttgga
     3421 tgacatgtga attttggtat ctagataagg ctttcgccat cttttttgta caacacagtc
     3481 actaattgtc tgaaggttta aatgacggtc tatgctattt ccccactccc cccaaaataa
     3541 aacatctaat attttagcta tagaatcact aaggcattga tcaaggatgt actttgccaa
     3601 ataagtattt ggtaacatca cttaacaagt tgatcgtgta ttgattctgt taacatatgt
     3661 gaacctgaaa agtaaagtta ccaaaagcga
//
