LOCUS       NM_018082               4183 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens RNA polymerase III subunit B (POLR3B), transcript
            variant 1, mRNA.
ACCESSION   NM_018082
VERSION     NM_018082.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Geroldi,A., Tozza,S., Fiorillo,C., Nolano,M., Fossa,P., Vitale,F.,
            Domi,R., Gaudio,A., Mammi,A., Patrone,S., Barbera,A., Origone,P.,
            Ponti,C., Sanguineri,F., Zara,F., Cataldi,M., Salpietro,V.,
            Venturi,C.B., Massucco,S., Schenone,A., Manganelli,F., Mandich,P.,
            Bellone,E. and Gotta,F.
  TITLE     A novel de novo variant in POLR3B gene associated with a primary
            axonal involvement of the largest nerve fibers
  JOURNAL   J Peripher Nerv Syst 28 (4), 620-628 (2023)
   PUBMED   37897416
  REMARK    GeneRIF: A novel de novo variant in POLR3B gene associated with a
            primary axonal involvement of the largest nerve fibers.
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Bai,H., Li,D., Zheng,Y. and Jiang,X.
  TITLE     Case report: Biallelic variants in POLR3B gene lead to 4H
            leukodystrophy from the study of brother and sister
  JOURNAL   Medicine (Baltimore) 101 (34), e30350 (2022)
   PUBMED   36042647
  REMARK    GeneRIF: Case report: Biallelic variants in POLR3B gene lead to 4H
            leukodystrophy from the study of brother and sister.
REFERENCE   3  (bases 1 to 4183)
  AUTHORS   Tetreault,M., Choquet,K., Orcesi,S., Tonduti,D., Balottin,U.,
            Teichmann,M., Fribourg,S., Schiffmann,R., Brais,B., Vanderver,A.
            and Bernard,G.
  TITLE     Recessive mutations in POLR3B, encoding the second largest subunit
            of Pol III, cause a rare hypomyelinating leukodystrophy
  JOURNAL   Am J Hum Genet 89 (5), 652-655 (2011)
   PUBMED   22036172
  REMARK    GeneRIF: Recessive mutations in POLR3B, encoding the second largest
            subunit of Pol III, cause a rare hypomyelinating leukodystrophy
REFERENCE   4  (bases 1 to 4183)
  AUTHORS   Saitsu,H., Osaka,H., Sasaki,M., Takanashi,J., Hamada,K.,
            Yamashita,A., Shibayama,H., Shiina,M., Kondo,Y., Nishiyama,K.,
            Tsurusaki,Y., Miyake,N., Doi,H., Ogata,K., Inoue,K. and
            Matsumoto,N.
  TITLE     Mutations in POLR3A and POLR3B encoding RNA Polymerase III subunits
            cause an autosomal-recessive hypomyelinating leukoencephalopathy
  JOURNAL   Am J Hum Genet 89 (5), 644-651 (2011)
   PUBMED   22036171
  REMARK    GeneRIF: Mutations in POLR3A and POLR3B encoding RNA Polymerase III
            subunits cause an autosomal-recessive hypomyelinating
            leukoencephalopathy
REFERENCE   5  (bases 1 to 4183)
  AUTHORS   Shen,E., Lei,Y., Liu,Q., Zheng,Y., Song,C., Marc,J., Wang,Y.,
            Sun,L. and Liang,Q.
  TITLE     Identification and characterization of INMAP, a novel interphase
            nucleus and mitotic apparatus protein that is involved in spindle
            formation and cell cycle progression
  JOURNAL   Exp Cell Res 315 (7), 1100-1116 (2009)
   PUBMED   19331820
  REMARK    GeneRIF: Results suggest that INMAP is a novel protein that plays
            essential role in spindle formation and cell-cycle progression.
REFERENCE   6  (bases 1 to 4183)
  AUTHORS   Hu,P., Wu,S., Sun,Y., Yuan,C.C., Kobayashi,R., Myers,M.P. and
            Hernandez,N.
  TITLE     Characterization of human RNA polymerase III identifies orthologues
            for Saccharomyces cerevisiae RNA polymerase III subunits
  JOURNAL   Mol Cell Biol 22 (22), 8044-8055 (2002)
   PUBMED   12391170
  REMARK    GeneRIF: Data describe the purification and identification of RNA
            polymerase III subunits RPC2 and RPC5.
REFERENCE   7  (bases 1 to 4183)
  AUTHORS   Vanderver,A., Tonduti,D., Schiffmann,R., Schmidt,J. and van der
            Knaap,M.S.
  TITLE     Leukodystrophy Overview - RETIRED CHAPTER, FOR HISTORICAL REFERENCE
            ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   24501781
REFERENCE   8  (bases 1 to 4183)
  AUTHORS   Bernard,G. and Vanderver,A.
  TITLE     POLR3-Related Leukodystrophy
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   22855961
REFERENCE   9  (bases 1 to 4183)
  AUTHORS   Balasubramanian,R. and Crowley,W.F. Jr.
  TITLE     Isolated Gonadotropin-Releasing Hormone (GnRH) Deficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301509
REFERENCE   10 (bases 1 to 4183)
  AUTHORS   Perlman,S.
  TITLE     Hereditary Ataxia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301317
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX537447.1 and BC033724.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_018082.5.
            
            Summary: This gene encodes the second largest subunit of RNA
            polymerase III, the polymerase responsible for synthesizing
            transfer and small ribosomal RNAs in eukaryotes. The largest
            subunit and the encoded protein form the catalytic center of RNA
            polymerase III. Mutations in this gene are a cause of
            hypomyelinating leukodystrophy. Alternatively spliced transcript
            variants encoding multiple isoforms have been observed for this
            gene. [provided by RefSeq, Dec 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX537447.1, SRR1803615.228111.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000228347.9/ ENSP00000228347.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3125              BX537447.1         39-3163
            3126-4183           BC033724.2         1269-2326
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q23.3"
     gene            1..4183
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /note="RNA polymerase III subunit B"
                     /db_xref="GeneID:55703"
                     /db_xref="HGNC:HGNC:30348"
                     /db_xref="MIM:614366"
     exon            1..204
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..3534
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /EC_number="2.7.7.6"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DNA-directed RNA polymerase III subunit B; RNA polymerase
                     III subunit C2; polymerase (RNA) III (DNA directed)
                     polypeptide B; DNA-directed RNA polymerase III subunit
                     RPC2; DNA-directed RNA polymerase III 127.6 kDa
                     polypeptide; interphase nucleus and mitotic apparatus
                     associated protein; polymerase (RNA) III subunit B"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase III subunit RPC2
                     isoform 1"
                     /protein_id="NP_060552.4"
                     /db_xref="CCDS:CCDS9105.1"
                     /db_xref="GeneID:55703"
                     /db_xref="HGNC:HGNC:30348"
                     /db_xref="MIM:614366"
                     /translation="MDVLAEEFGNLTPEQLAAPIPTVEEKWRLLPAFLKVKGLVKQHI
                     DSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHEC
                     RLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNE
                     CPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGAVGASVTSSTHEKKSRTNMAV
                     KQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQ
                     IFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIY
                     TAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVI
                     PKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALG
                     MMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMED
                     GPIVKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVNTFRLMRRAG
                     YINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDF
                     LHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPR
                     NTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIEFEKLPAGQNAT
                     VAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAA
                     TRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPI
                     TYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQE
                     DMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDV
                     CEDLVRHGYNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAV
                     LTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGY
                     SGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLSKYNE"
     exon            205..237
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            238..294
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            295..359
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            360..435
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            436..536
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            537..628
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            629..746
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            747..855
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            856..978
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1098
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1233
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1395
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1396..1596
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..1759
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1913
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1914..1988
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1989..2087
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2088..2215
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2216..2425
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2426..2584
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2585..2702
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2703..2845
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2846..2949
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2950..3116
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3117..3230
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3231..3404
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3405..4183
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4008..4013
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /note="hexamer: ATTAAA"
     polyA_site      4034
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /note="major polyA site"
     regulatory      4063..4068
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /note="hexamer: AATGAA"
     polyA_site      4091
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
     polyA_site      4183
                     /gene="POLR3B"
                     /gene_synonym="C128; CMT1I; HLD8; INMAP; RPC2"
                     /note="major polyA site"
ORIGIN      
        1 gtctctagct aacacgcacg gcggggacag tttaggcctc cgcgcaccgt tcgccgggag
       61 tcttgcagtt tgcttggtgc agggaaggcg ggcgcggagg ttctatctgt ttcttcctcc
      121 ttcgtgagca gcatggacgt gctagcggag gagtttggga acctgactcc ggagcagctg
      181 gcggcgccga tcccgactgt agaggaaaaa tggaggctgc ttccagcatt tttaaaggtg
      241 aaaggccttg tgaaacagca tatagattca tttaactatt tcattaatgt agagataaag
      301 aagataatga aagccaatga aaaggttaca agtgacgctg accctatgtg gtacttaaaa
      361 tatcttaata tctatgttgg gcttcctgat gttgaagaaa gcttcaatgt aactagacca
      421 gtgtcccctc atgagtgccg tttgagagac atgacatact ctgcccctat tacagtggat
      481 attgaatata cccgaggcag ccagaggatc atccgcaatg ccttacctat cggcagaatg
      541 cccataatgc tacgtagttc aaactgtgtt cttacaggaa aaacgccagc agaatttgcc
      601 aaactgaacg aatgtccctt agatccaggt ggctacttca ttgttaaagg agtagaaaaa
      661 gttattctta tccaagagca gctgtctaag aacaggatca tcgtggaggc tgatagaaaa
      721 ggggctgttg gagcttcagt taccagctct acccatgaga aaaaaagcag aaccaatatg
      781 gctgtgaaac aaggacgatt ttatttgagg cataatactt tgtcagaaga tatacccatt
      841 gtcatcatat ttaaggccat gggtgttgag agtgaccagg aaattgtgca gatgattgga
      901 acagaggagc acgtgatggc tgcatttggg cccagtctgg aagagtgcca gaaagctcag
      961 attttcacac agatgcaggc attaaaatat atagggaaca aagtaagaag gcaaaggatg
     1021 tggggaggtg gaccaaagaa aaccaaaata gaagaagcaa gagagctcct ggcttccacc
     1081 attctgaccc atgtcccagt taaggaattc aatttccgag ccaaatgtat ctatactgca
     1141 gtgatggtgc gaagagttat tctggcccaa ggagataata aagttgacga cagagattat
     1201 tatggtaaca agcgactgga attggcagga cagcttttat ctcttctttt tgaagacttg
     1261 ttcaaaaaat ttaattctga aatgaaaaag attgccgacc aggtgattcc taagcaaaga
     1321 gcagcccagt ttgatgttgt caaacacatg cgccaagacc agatcaccaa tggcatggtg
     1381 aatgctattt ctaccggaaa ttggtcttta aagagattta aaatggaccg ccagggtgta
     1441 acccaagtgc tgtctcgctt gtcatatata tccgcactgg gcatgatgac aagaatctct
     1501 tcccagtttg aaaaaacgag aaaagtgagt ggtcctcgct ccctccagcc atctcagtgg
     1561 ggaatgctgt gtccttcgga cactcctgaa ggagaggcat gtggtttggt taaaaacttg
     1621 gcccttatga cacacatcac aactgatatg gaagatggac ccattgttaa attagccagt
     1681 aacttgggag tagaagatgt gaatttatta tgtggggaag agctctctta cccaaatgtg
     1741 tttcttgtct ttcttaatgg taacatctta ggtgtcattc gagaccacaa aaagctagtg
     1801 aatacatttc gactcatgag aagagcagga tatatcaatg aatttgtttc catctcaaca
     1861 aatcttacag atcgatgtgt ctatatttct tctgatgggg gaaggctatg cagaccctac
     1921 ataattgtca agaaacagaa gccagcagtc acaaataaac atatggaaga gctggcccaa
     1981 gggtacagga attttgaaga tttcttacat gagagtctgg ttgaatattt agatgtgaat
     2041 gaagaaaatg attgtaacat tgcactgtac gaacacacaa ttaataaaga caccacccac
     2101 ttggagattg aacccttcac tcttctcggc gtgtgtgctg gacttatccc ataccctcac
     2161 cataaccagt caccgagaaa cacttatcag tgtgccatgg ggaaacaagc catgggtact
     2221 ataggataca accagcgaaa cagaattgat actctcatgt atctactagc atatccacaa
     2281 aaacccatgg ttaagacaaa aaccattgaa ttgatagaat ttgagaaact gccagctgga
     2341 cagaatgcaa cagttgctgt gatgagctat agtggctatg atattgaaga tgctcttgtt
     2401 ttaaacaagg cctctttaga cagaggcttt gggcgttgcc ttgtatataa aaatgctaaa
     2461 tgtacgttga aacgatacac caatcagact tttgataaag tgatggggcc catgttggat
     2521 gctgctacaa ggaaacctat ctggcgacat gaaatcttag atgcagatgg tatttgttct
     2581 ccaggtgaga aagtagaaaa caaacaagtg cttgtaaata agtccatgcc cacagtgact
     2641 cagattcctt tggaaggaag taatgtacca cagcaaccac agtacaaaga tgtacccata
     2701 acctacaaag gagcaacaga ctcatatatt gaaaaagtga tgatatcttc aaatgctgaa
     2761 gatgcttttc tgatcaaaat gctgctgaga cagacaaggc gtccagaaat tggagacaaa
     2821 ttcagcagtc gtcatgggca aaaaggtgtt tgtggcttga tcgtccccca ggaagacatg
     2881 ccattttgtg attctggcat ctgtccggac atcatcatga acccacacgg cttcccatca
     2941 cgaatgacgg tggggaagct cattgagctg ctggctggca aggccggtgt gctggacggc
     3001 agattccact acggcactgc gtttggaggc agtaaagtga aggatgtgtg tgaggacctc
     3061 gttcgccatg gttataacta cttggggaaa gactatgtta catccggcat cacaggtgag
     3121 cccttagaag catacatcta ttttggcccc gtgtactatc agaagctgaa acacatggtg
     3181 ctagataaaa tgcatgcccg ggcccggggc ccacgagccg tccttaccag gcaacccact
     3241 gaaggacggt ctcgtgatgg tggcttgcgt ctcggggaaa tggaacgtga ctgtttaatc
     3301 ggttatggag ccagtatgct tttgctagag agactaatga tttcaagtga tgcctttgag
     3361 gttgatgtct gtgggcagtg tggacttctg gggtattctg gctggtgcca ttactgcaag
     3421 tcatcctgcc acgtgtcttc cctccgtatt ccgtatgcct gcaagctgct cttccaggaa
     3481 ctacagtcta tgaacatcat ccccaggtta aaactgtcca agtacaatga atgaggatgg
     3541 aaaaaatgat tattaaagag aacaagtgat acatccaatg caacggaaag cagaagggat
     3601 ttaggactac gtctcctcct gtgaagaatt cccttgcgta ttctctctct aaaacaacca
     3661 aaaaaaaatg gagaggcttt ttatatactc taagactggc taaacaacct tgatcattga
     3721 gcctcgagcc atgggagaga tgctgaccat gtggactgca aggctgcttg attcacagat
     3781 ggatgtgacc taaaggataa ataagctatt acttatgtgc tgatctcttg acattcactc
     3841 attagaagac cttactcctt caagcaaatg tttggggtca aatttaccat atcttctggc
     3901 taaccatatt caagattctt ctgaaacttg gaggatgtaa agaatccatt tgatttggtc
     3961 agcctggctt ttgtcgtggt ggctggctcg gataaatttt cccaacaatt aaatcttgcc
     4021 tttacacacc caaactttgt aattttagtc ttggtgaaat ataatgaatt tgttcctacc
     4081 ttgtcaagca agaatgtcgt cttctcctat ggactcaatt gctattattt taaacctgca
     4141 tgattgtacc atgcaatact attcgttaaa tatctgaatc taa
//
