LOCUS       NM_005034                947 bp    mRNA    linear   PRI 13-APR-2024
DEFINITION  Homo sapiens RNA polymerase II, I and III subunit K (POLR2K), mRNA.
ACCESSION   NM_005034
VERSION     NM_005034.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Daiss,J.L., Pilsl,M., Straub,K., Bleckmann,A., Hocherl,M.,
            Heiss,F.B., Abascal-Palacios,G., Ramsay,E.P., Tluckova,K.,
            Mars,J.C., Furtges,T., Bruckmann,A., Rudack,T., Bernecky,C.,
            Lamour,V., Panov,K., Vannini,A., Moss,T. and Engel,C.
  TITLE     The human RNA polymerase I structure reveals an HMG-like docking
            domain specific to metazoans
  JOURNAL   Life Sci Alliance 5 (11), e202201568 (2022)
   PUBMED   36271492
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  AUTHORS   Hou,H., Li,Y., Wang,M., Liu,A., Yu,Z., Chen,K., Zhao,D. and Xu,Y.
  TITLE     Structural insights into RNA polymerase III-mediated transcription
            termination through trapping poly-deoxythymidine
  JOURNAL   Nat Commun 12 (1), 6135 (2021)
   PUBMED   34675218
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 947)
  AUTHORS   Zhao,D., Liu,W., Chen,K., Wu,Z., Yang,H. and Xu,Y.
  TITLE     Structure of the human RNA polymerase I elongation complex
  JOURNAL   Cell Discov 7 (1), 97 (2021)
   PUBMED   34671025
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 947)
  AUTHORS   Ramsay,E.P., Abascal-Palacios,G., Daiss,J.L., King,H., Gouge,J.,
            Pilsl,M., Beuron,F., Morris,E., Gunkel,P., Engel,C. and Vannini,A.
  TITLE     Structure of human RNA polymerase III
  JOURNAL   Nat Commun 11 (1), 6409 (2020)
   PUBMED   33335104
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 947)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 947)
  AUTHORS   Kato,H., Sumimoto,H., Pognonec,P., Chen,C.H., Rosen,C.A. and
            Roeder,R.G.
  TITLE     HIV-1 Tat acts as a processivity factor in vitro in conjunction
            with cellular elongation factors
  JOURNAL   Genes Dev 6 (4), 655-666 (1992)
   PUBMED   1559613
REFERENCE   7  (bases 1 to 947)
  AUTHORS   Jang,K.L., Collins,M.K. and Latchman,D.S.
  TITLE     The human immunodeficiency virus tat protein increases the
            transcription of human Alu repeated sequences by increasing the
            activity of the cellular transcription factor TFIIIC
  JOURNAL   J Acquir Immune Defic Syndr (1988) 5 (11), 1142-1147 (1992)
   PUBMED   1403646
REFERENCE   8  (bases 1 to 947)
  AUTHORS   Jacob,G.A., Luse,S.W. and Luse,D.S.
  TITLE     Abortive initiation is increased only for the weakest members of a
            set of down mutants of the adenovirus 2 major late promoter
  JOURNAL   J Biol Chem 266 (33), 22537-22544 (1991)
   PUBMED   1939271
REFERENCE   9  (bases 1 to 947)
  AUTHORS   Southgate,C., Zapp,M.L. and Green,M.R.
  TITLE     Activation of transcription by HIV-1 Tat protein tethered to
            nascent RNA through another protein
  JOURNAL   Nature 345 (6276), 640-642 (1990)
   PUBMED   2190099
REFERENCE   10 (bases 1 to 947)
  AUTHORS   Conaway,R.C. and Conaway,J.W.
  TITLE     ATP activates transcription initiation from promoters by RNA
            polymerase II in a reversible step prior to RNA synthesis
  JOURNAL   J Biol Chem 263 (6), 2962-2968 (1988)
   PUBMED   2449431
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC018157.1 and AC025647.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_005034.3.
            
            Summary: This gene encodes one of the smallest subunits of RNA
            polymerase II, the polymerase responsible for synthesizing
            messenger RNA in eukaryotes. This subunit is shared by the other
            two DNA-directed RNA polymerases. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BM807282.1, BG708922.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000353107.8/ ENSP00000342889.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-557               BC018157.1         26-582
            558-947             AC025647.9         47300-47689
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q22.2"
     gene            1..947
                     /gene="POLR2K"
                     /gene_synonym="ABC10-alpha; hRPB7.0; hsRPB10a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /note="RNA polymerase II, I and III subunit K"
                     /db_xref="GeneID:5440"
                     /db_xref="HGNC:HGNC:9198"
                     /db_xref="MIM:606033"
     exon            1..74
                     /gene="POLR2K"
                     /gene_synonym="ABC10-alpha; hRPB7.0; hsRPB10a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /inference="alignment:Splign:2.1.0"
     exon            75..144
                     /gene="POLR2K"
                     /gene_synonym="ABC10-alpha; hRPB7.0; hsRPB10a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..260
                     /gene="POLR2K"
                     /gene_synonym="ABC10-alpha; hRPB7.0; hsRPB10a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /EC_number="2.7.7.6"
                     /note="DNA directed RNA polymerases I, II, and III 7.0 kda
                     polypeptide; polymerase (RNA) II subunit K; RNA polymerase
                     II 7.0 kDa subunit; DNA-directed RNA polymerase II subunit
                     K; RNA polymerases I, II, and III subunit ABC4; polymerase
                     (RNA) II (DNA directed) polypeptide K, 7.0kDa"
                     /codon_start=1
                     /product="DNA-directed RNA polymerases I, II, and III
                     subunit RPABC4"
                     /protein_id="NP_005025.1"
                     /db_xref="CCDS:CCDS6285.1"
                     /db_xref="GeneID:5440"
                     /db_xref="HGNC:HGNC:9198"
                     /db_xref="MIM:606033"
                     /translation="MDTQKDVQPPKQQPMIYICGECHTENEIKSRDPIRCRECGYRIM
                     YKKRTKRLVVFDAR"
     exon            145..237
                     /gene="POLR2K"
                     /gene_synonym="ABC10-alpha; hRPB7.0; hsRPB10a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /inference="alignment:Splign:2.1.0"
     exon            238..947
                     /gene="POLR2K"
                     /gene_synonym="ABC10-alpha; hRPB7.0; hsRPB10a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /inference="alignment:Splign:2.1.0"
     regulatory      537..542
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR2K"
                     /gene_synonym="ABC10-alpha; hRPB7.0; hsRPB10a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /note="hexamer: AATAAA"
     polyA_site      557
                     /gene="POLR2K"
                     /gene_synonym="ABC10-alpha; hRPB7.0; hsRPB10a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /note="major polyA site"
     regulatory      927..932
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR2K"
                     /gene_synonym="ABC10-alpha; hRPB7.0; hsRPB10a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /note="hexamer: AATAAA"
     polyA_site      947
                     /gene="POLR2K"
                     /gene_synonym="ABC10-alpha; hRPB7.0; hsRPB10a; RPABC4;
                     RPB10alpha; RPB12; RPB7.0"
                     /note="major polyA site"
ORIGIN      
        1 ggaagttggt ctcgacacct ggactagccg ggttgtattt ggaaacgcgg agtgagtttt
       61 tccgtgctgt gtaggggcta acaatggaca cccagaagga cgttcaacct ccaaagcagc
      121 aaccaatgat atatatctgt ggagagtgtc acacagaaaa tgaaataaaa tctagggatc
      181 caatcagatg cagagaatgt ggatacagaa taatgtacaa gaaaaggact aaaagattgg
      241 tcgtttttga tgctcgatga atgctgggaa ttcagaggaa tgtcttcact tatacttgga
      301 tttgctctct tcccatttct gattgttgta tagctttcga ttttgcttac agtagttccc
      361 ccttatcttc gggagataca ttccaaggcc cccagtgaac tcctgaaacc tcaaacagta
      421 ccaaaccttt atacactgtt ttttccatat atatatacct atgataaagt ataatgtata
      481 aattaagcat agcaagagat taataataat gtaatagaac aatgataaca tactataata
      541 aaagttatgt gaatgtggtt ggtctctctt gctttcaaaa tatcttcttg tacagtactc
      601 acctatttta gaatgtggtt gactacaggt aaccaaaacc acagaaaggg aaactttgga
      661 tgaggggggc actactgtac ttaggaatac aactatatac atatgatttt atttttaaga
      721 ccatattata tttgggtatc tactaatatt ttgtataaag caattttttg ttccattacg
      781 tgactttttg ttttattgta tatgtaattt aacacacaat aaagggtaaa gttgcttccc
      841 caaaccacac ttttaatcaa aacctagaat catctgcagt ccttgttaaa aatgcaggtt
      901 tctagaaccc tctgaagttc tgattaaata aatttattgc aaaccac
//
