LOCUS       NM_032959                495 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens RNA polymerase II subunit J2 (POLR2J2), mRNA.
ACCESSION   NM_032959
VERSION     NM_032959.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
REFERENCE   2  (bases 1 to 495)
  AUTHORS   Shpakovskii,D.G., Shematorova,E.K. and Shpakovskii,G.V.
  TITLE     Human PMS2 gene family: origin, molecular evolution, and biological
            implications
  JOURNAL   Dokl Biochem Biophys 408, 175-179 (2006)
   PUBMED   16913423
REFERENCE   3  (bases 1 to 495)
  AUTHORS   Benga,W.J., Grandemange,S., Shpakovski,G.V., Shematorova,E.K.,
            Kedinger,C. and Vigneron,M.
  TITLE     Distinct regions of RPB11 are required for heterodimerization with
            RPB3 in human and yeast RNA polymerase II
  JOURNAL   Nucleic Acids Res 33 (11), 3582-3590 (2005)
   PUBMED   15987790
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 495)
  AUTHORS   Durrin,L.K. and Krontiris,T.G.
  TITLE     The thymocyte-specific MAR binding protein, SATB1, interacts in
            vitro with a novel variant of DNA-directed RNA polymerase II,
            subunit 11
  JOURNAL   Genomics 79 (6), 809-817 (2002)
   PUBMED   12036295
REFERENCE   5  (bases 1 to 495)
  AUTHORS   Grandemange,S., Schaller,S., Yamano,S., Du Manoir,S.,
            Shpakovski,G.V., Mattei,M.G., Kedinger,C. and Vigneron,M.
  TITLE     A human RNA polymerase II subunit is encoded by a recently
            generated multigene family
  JOURNAL   BMC Mol Biol 2, 14 (2001)
   PUBMED   11747469
REFERENCE   6  (bases 1 to 495)
  AUTHORS   Cramer,P., Bushnell,D.A., Fu,J., Gnatt,A.L., Maier-Davis,B.,
            Thompson,N.E., Burgess,R.R., Edwards,A.M., David,P.R. and
            Kornberg,R.D.
  TITLE     Architecture of RNA polymerase II and implications for the
            transcription mechanism
  JOURNAL   Science 288 (5466), 640-649 (2000)
   PUBMED   10784442
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC105052.3.
            
            On Oct 21, 2021 this sequence version replaced NM_032959.6.
            
            Summary: This gene is a member of the RNA polymerase II subunit 11
            gene family, which includes three genes in a cluster on chromosome
            7q22.1 and a pseudogene on chromosome 7p13. The founding member of
            this family, DNA directed RNA polymerase II polypeptide J, has been
            shown to encode a subunit of RNA polymerase II, the polymerase
            responsible for synthesizing messenger RNA in eukaryotes. This
            locus produces multiple, alternatively spliced transcripts that
            potentially express isoforms with distinct C-termini compared to
            DNA directed RNA polymerase II polypeptide J. Most or all variants
            are spliced to include additional non-coding exons at the 3' end
            which makes them candidates for nonsense-mediated decay (NMD).
            Consequently, it is not known if this locus expresses a protein or
            proteins in vivo. [provided by RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DB150130.1, SRR14038192.804778.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2144333, SAMEA2146411
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000333432.9/ ENSP00000330898.7
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-112               AC105052.3         85293-85404         c
            113-202             AC105052.3         82656-82745         c
            203-377             AC105052.3         80831-81005         c
            378-495             AC105052.3         79787-79904         c
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q22.1"
     gene            1..495
                     /gene="POLR2J2"
                     /gene_synonym="HRPB11B; RPB11b1"
                     /note="RNA polymerase II subunit J2"
                     /db_xref="GeneID:246721"
                     /db_xref="HGNC:HGNC:23208"
                     /db_xref="MIM:609881"
     exon            1..112
                     /gene="POLR2J2"
                     /gene_synonym="HRPB11B; RPB11b1"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..410
                     /gene="POLR2J2"
                     /gene_synonym="HRPB11B; RPB11b1"
                     /note="DNA directed RNA polymerase II polypeptide
                     J-related; polymerase (RNA) II subunit J2; RNA polymerase
                     II subunit B11-b1; polymerase (RNA) II (DNA directed)
                     polypeptide J2; DNA-directed RNA polymerase II subunit J2"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase II subunit RPB11-b1"
                     /protein_id="NP_116581.3"
                     /db_xref="CCDS:CCDS43627.1"
                     /db_xref="GeneID:246721"
                     /db_xref="HGNC:HGNC:23208"
                     /db_xref="MIM:609881"
                     /translation="MNAPPAFESFLLFEGEKITINKDTKVPKACLFTINKEDHTLGNI
                     IKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEE
                     RFRTCLLPLRLLP"
     exon            113..202
                     /gene="POLR2J2"
                     /gene_synonym="HRPB11B; RPB11b1"
                     /inference="alignment:Splign:2.1.0"
     exon            203..377
                     /gene="POLR2J2"
                     /gene_synonym="HRPB11B; RPB11b1"
                     /inference="alignment:Splign:2.1.0"
     exon            378..495
                     /gene="POLR2J2"
                     /gene_synonym="HRPB11B; RPB11b1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 attagggctc tggtggcggc ggtggcggac acttggggtc tggacgcaac ggcggcggga
       61 gcatgaacgc ccctccagcc ttcgagtcgt tcttgctctt cgagggcgag aagatcacca
      121 ttaacaagga caccaaggta cccaaggcct gcttattcac catcaacaaa gaagaccaca
      181 cactgggaaa catcattaaa tcacaactcc taaaagaccc gcaagtgcta tttgctggct
      241 acaaagtccc ccaccccttg gagcacaaga tcatcatccg agtgcagacc acgccggact
      301 acagccccca ggaagccttt accaacgcca tcaccgacct catcagtgag ctgtccctgc
      361 tggaggagcg cttccggacg tgcctgcttc cccttcgcct tctgccgtga ttgtcagttt
      421 cctgaggcct ccccagccac gcttcctgta cagcctgcag aactgtgagt caattaaacc
      481 tcttttcttc ataaa
//
