LOCUS       NM_006234                937 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens RNA polymerase II subunit J (POLR2J), transcript
            variant 1, mRNA.
ACCESSION   NM_006234 XM_001126227
VERSION     NM_006234.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 937)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 937)
  AUTHORS   Wang,J., Huo,K., Ma,L., Tang,L., Li,D., Huang,X., Yuan,Y., Li,C.,
            Wang,W., Guan,W., Chen,H., Jin,C., Wei,J., Zhang,W., Yang,Y.,
            Liu,Q., Zhou,Y., Zhang,C., Wu,Z., Xu,W., Zhang,Y., Liu,T., Yu,D.,
            Zhang,Y., Chen,L., Zhu,D., Zhong,X., Kang,L., Gan,X., Yu,X., Ma,Q.,
            Yan,J., Zhou,L., Liu,Z., Zhu,Y., Zhou,T., He,F. and Yang,X.
  TITLE     Toward an understanding of the protein interaction network of the
            human liver
  JOURNAL   Mol Syst Biol 7, 536 (2011)
   PUBMED   21988832
  REMARK    Erratum:[Mol Syst Biol. 2017 Dec 18;13(12):965. doi:
            10.15252/msb.20178107.. PMID: 29254952]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 937)
  AUTHORS   Proshkin,S.A., Shematorova,E.K., Souslova,E.A., Proshkina,G.M. and
            Shpakovski,G.V.
  TITLE     A minor isoform of the human RNA polymerase II subunit hRPB11
            (POLR2J) interacts with several components of the translation
            initiation factor eIF3
  JOURNAL   Biochemistry (Mosc) 76 (8), 976-980 (2011)
   PUBMED   22022972
  REMARK    GeneRIF: POLR2J interacts with three different subunits of eIF3,
            eIF3a, eIF3i, and eIF3m.
REFERENCE   5  (bases 1 to 937)
  AUTHORS   Michiels,S., Laplanche,A., Boulet,T., Dessen,P., Guillonneau,B.,
            Mejean,A., Desgrandchamps,F., Lathrop,M., Sarasin,A. and
            Benhamou,S.
  TITLE     Genetic polymorphisms in 85 DNA repair genes and bladder cancer
            risk
  JOURNAL   Carcinogenesis 30 (5), 763-768 (2009)
   PUBMED   19237606
  REMARK    GeneRIF: Observational study of gene-disease association, gene-gene
            interaction, and gene-environment interaction. (HuGE Navigator)
REFERENCE   6  (bases 1 to 937)
  AUTHORS   Wu-Baer,F., Sigman,D. and Gaynor,R.B.
  TITLE     Specific binding of RNA polymerase II to the human immunodeficiency
            virus trans-activating region RNA is regulated by cellular
            cofactors and Tat
  JOURNAL   Proc Natl Acad Sci U S A 92 (16), 7153-7157 (1995)
   PUBMED   7638159
REFERENCE   7  (bases 1 to 937)
  AUTHORS   Kato,H., Sumimoto,H., Pognonec,P., Chen,C.H., Rosen,C.A. and
            Roeder,R.G.
  TITLE     HIV-1 Tat acts as a processivity factor in vitro in conjunction
            with cellular elongation factors
  JOURNAL   Genes Dev 6 (4), 655-666 (1992)
   PUBMED   1559613
REFERENCE   8  (bases 1 to 937)
  AUTHORS   Jacob,G.A., Luse,S.W. and Luse,D.S.
  TITLE     Abortive initiation is increased only for the weakest members of a
            set of down mutants of the adenovirus 2 major late promoter
  JOURNAL   J Biol Chem 266 (33), 22537-22544 (1991)
   PUBMED   1939271
REFERENCE   9  (bases 1 to 937)
  AUTHORS   Southgate,C., Zapp,M.L. and Green,M.R.
  TITLE     Activation of transcription by HIV-1 Tat protein tethered to
            nascent RNA through another protein
  JOURNAL   Nature 345 (6276), 640-642 (1990)
   PUBMED   2190099
REFERENCE   10 (bases 1 to 937)
  AUTHORS   Conaway,R.C. and Conaway,J.W.
  TITLE     ATP activates transcription initiation from promoters by RNA
            polymerase II in a reversible step prior to RNA synthesis
  JOURNAL   J Biol Chem 263 (6), 2962-2968 (1988)
   PUBMED   2449431
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM545237.1 and BC065711.1.
            
            On Aug 13, 2020 this sequence version replaced NM_006234.5.
            
            Summary: This gene encodes a subunit of RNA polymerase II, the
            polymerase responsible for synthesizing messenger RNA in
            eukaryotes. The product of this gene exists as a heterodimer with
            another polymerase subunit; together they form a core subassembly
            unit of the polymerase. Two similar genes are located nearby on
            chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DA125686.1, DA912594.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000292614.10/ ENSP00000292614.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                BM545237.1         131-145
            16-558              BC065711.1         6-548
            559-937             BC065711.1         579-957
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q22.1"
     gene            1..937
                     /gene="POLR2J"
                     /gene_synonym="hRPB14; POLR2J1; RPB11; RPB11A; RPB11m"
                     /note="RNA polymerase II subunit J"
                     /db_xref="GeneID:5439"
                     /db_xref="HGNC:HGNC:9197"
                     /db_xref="MIM:604150"
     exon            1..115
                     /gene="POLR2J"
                     /gene_synonym="hRPB14; POLR2J1; RPB11; RPB11A; RPB11m"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..416
                     /gene="POLR2J"
                     /gene_synonym="hRPB14; POLR2J1; RPB11; RPB11A; RPB11m"
                     /EC_number="2.7.7.6"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DNA-directed RNA polymerase II subunit RPB11-a; RNA
                     polymerase II subunit B11-a; RNA polymerase II 13.3 kDa
                     subunit; polymerase (RNA) II subunit J; DNA-directed RNA
                     polymerase II subunit J-1; polymerase (RNA) II (DNA
                     directed) polypeptide J, 13.3kDa"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase II subunit RPB11-a
                     isoform 1"
                     /protein_id="NP_006225.1"
                     /db_xref="CCDS:CCDS5724.1"
                     /db_xref="GeneID:5439"
                     /db_xref="HGNC:HGNC:9197"
                     /db_xref="MIM:604150"
                     /translation="MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGN
                     IIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLE
                     ERFRVAIKDKQEGIE"
     exon            116..205
                     /gene="POLR2J"
                     /gene_synonym="hRPB14; POLR2J1; RPB11; RPB11A; RPB11m"
                     /inference="alignment:Splign:2.1.0"
     exon            206..380
                     /gene="POLR2J"
                     /gene_synonym="hRPB14; POLR2J1; RPB11; RPB11A; RPB11m"
                     /inference="alignment:Splign:2.1.0"
     exon            381..937
                     /gene="POLR2J"
                     /gene_synonym="hRPB14; POLR2J1; RPB11; RPB11A; RPB11m"
                     /inference="alignment:Splign:2.1.0"
     regulatory      588..593
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR2J"
                     /gene_synonym="hRPB14; POLR2J1; RPB11; RPB11A; RPB11m"
                     /note="hexamer: AATAAA"
     polyA_site      614
                     /gene="POLR2J"
                     /gene_synonym="hRPB14; POLR2J1; RPB11; RPB11A; RPB11m"
                     /note="major polyA site"
     regulatory      918..923
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR2J"
                     /gene_synonym="hRPB14; POLR2J1; RPB11; RPB11A; RPB11m"
                     /note="hexamer: AATAAA"
     polyA_site      925
                     /gene="POLR2J"
                     /gene_synonym="hRPB14; POLR2J1; RPB11; RPB11A; RPB11m"
     polyA_site      937
                     /gene="POLR2J"
                     /gene_synonym="hRPB14; POLR2J1; RPB11; RPB11A; RPB11m"
ORIGIN      
        1 attagggctc tggtggcggc ggcggcggac ccttggggtc tggacgcaac ggcggcggga
       61 gcatgaacgc ccctccagcc ttcgagtcgt tcttgctctt cgagggcgag aagaagatca
      121 ccattaacaa ggacaccaag gtacccaatg cctgtttatt caccatcaac aaagaagacc
      181 acacactggg aaacatcatt aaatcacaac tcctaaaaga cccgcaagtg ctatttgctg
      241 gctacaaagt cccccacccc ttggagcaca agatcatcat ccgagtgcag accacgccgg
      301 actacagccc ccaggaagcc tttaccaacg ccatcaccga cctcatcagt gagctgtccc
      361 tgctggagga gcgctttcgg gtggccataa aagacaagca ggaaggaatt gagtaggggc
      421 cagagggggc tctgctcggc ctgtgagccc cgttcctacc tgtgcctgac cctccgctcc
      481 aggtaccaca ccgaggagag cggccggtcc cagccatggc ccgccttgtg gccacccctc
      541 accctgacac cgacgtgtcc tgtacataga ttaggtttta tattcctaat aaagtatagc
      601 gggagagacc tggatgtgga cttgagcagc ggtgacttcg caagcaaatg gattgtcagg
      661 cttgatgcag gcagatgacc tgtttcaggg gcgtccggct ggcagggatg aattcattct
      721 ggaccaaaga tccggggtcc aggggctgct gcgggggctg tgctgagccg gagagaagtg
      781 tgcaaaccca tgagctccca agagtctctg ctctagaagc ctcaactcct gggcctgcct
      841 gtcagtcaaa gcaggaacac ttcttcctgc ataactcgaa acacctttcc acaggcttct
      901 tgtccacagt agagtttaat aaaaatattc actgaaa
//
