LOCUS       NM_006232               1807 bp    mRNA    linear   PRI 13-APR-2024
DEFINITION  Homo sapiens RNA polymerase II, I and III subunit H (POLR2H),
            transcript variant 2, mRNA.
ACCESSION   NM_006232 XM_006713668
VERSION     NM_006232.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Daiss,J.L., Pilsl,M., Straub,K., Bleckmann,A., Hocherl,M.,
            Heiss,F.B., Abascal-Palacios,G., Ramsay,E.P., Tluckova,K.,
            Mars,J.C., Furtges,T., Bruckmann,A., Rudack,T., Bernecky,C.,
            Lamour,V., Panov,K., Vannini,A., Moss,T. and Engel,C.
  TITLE     The human RNA polymerase I structure reveals an HMG-like docking
            domain specific to metazoans
  JOURNAL   Life Sci Alliance 5 (11), e202201568 (2022)
   PUBMED   36271492
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Hou,H., Li,Y., Wang,M., Liu,A., Yu,Z., Chen,K., Zhao,D. and Xu,Y.
  TITLE     Structural insights into RNA polymerase III-mediated transcription
            termination through trapping poly-deoxythymidine
  JOURNAL   Nat Commun 12 (1), 6135 (2021)
   PUBMED   34675218
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1807)
  AUTHORS   Zhao,D., Liu,W., Chen,K., Wu,Z., Yang,H. and Xu,Y.
  TITLE     Structure of the human RNA polymerase I elongation complex
  JOURNAL   Cell Discov 7 (1), 97 (2021)
   PUBMED   34671025
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1807)
  AUTHORS   Ramsay,E.P., Abascal-Palacios,G., Daiss,J.L., King,H., Gouge,J.,
            Pilsl,M., Beuron,F., Morris,E., Gunkel,P., Engel,C. and Vannini,A.
  TITLE     Structure of human RNA polymerase III
  JOURNAL   Nat Commun 11 (1), 6409 (2020)
   PUBMED   33335104
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1807)
  AUTHORS   Paulsen,T., Shibata,Y., Kumar,P., Dillon,L. and Dutta,A.
  TITLE     Small extrachromosomal circular DNAs, microDNA, produce short
            regulatory RNAs that suppress gene expression independent of
            canonical promoters
  JOURNAL   Nucleic Acids Res 47 (9), 4586-4596 (2019)
   PUBMED   30828735
  REMARK    GeneRIF: We also show that endogenous microDNA associate with RNA
            polymerases subunits, POLR2H and POLR3F. Together, these results
            suggest that microDNA may modulate gene expression through the
            production of both known and novel regulatory small RNA.
REFERENCE   6  (bases 1 to 1807)
  AUTHORS   Kato,H., Sumimoto,H., Pognonec,P., Chen,C.H., Rosen,C.A. and
            Roeder,R.G.
  TITLE     HIV-1 Tat acts as a processivity factor in vitro in conjunction
            with cellular elongation factors
  JOURNAL   Genes Dev 6 (4), 655-666 (1992)
   PUBMED   1559613
REFERENCE   7  (bases 1 to 1807)
  AUTHORS   Jang,K.L., Collins,M.K. and Latchman,D.S.
  TITLE     The human immunodeficiency virus tat protein increases the
            transcription of human Alu repeated sequences by increasing the
            activity of the cellular transcription factor TFIIIC
  JOURNAL   J Acquir Immune Defic Syndr (1988) 5 (11), 1142-1147 (1992)
   PUBMED   1403646
REFERENCE   8  (bases 1 to 1807)
  AUTHORS   Jacob,G.A., Luse,S.W. and Luse,D.S.
  TITLE     Abortive initiation is increased only for the weakest members of a
            set of down mutants of the adenovirus 2 major late promoter
  JOURNAL   J Biol Chem 266 (33), 22537-22544 (1991)
   PUBMED   1939271
REFERENCE   9  (bases 1 to 1807)
  AUTHORS   Southgate,C., Zapp,M.L. and Green,M.R.
  TITLE     Activation of transcription by HIV-1 Tat protein tethered to
            nascent RNA through another protein
  JOURNAL   Nature 345 (6276), 640-642 (1990)
   PUBMED   2190099
REFERENCE   10 (bases 1 to 1807)
  AUTHORS   Conaway,R.C. and Conaway,J.W.
  TITLE     ATP activates transcription initiation from promoters by RNA
            polymerase II in a reversible step prior to RNA synthesis
  JOURNAL   J Biol Chem 263 (6), 2962-2968 (1988)
   PUBMED   2449431
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC078797.20.
            
            On Aug 13, 2020 this sequence version replaced NM_006232.4.
            
            Summary: The three eukaryotic RNA polymerases are complex
            multisubunit enzymes that play a central role in the transcription
            of nuclear genes. This gene encodes an essential and highly
            conserved subunit of RNA polymerase II that is shared by the other
            two eukaryotic DNA-directed RNA polymerases, I and III. Alternative
            splicing results in multiple transcript variants of this gene.
            [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 3' coding region, which results in a frameshift, compared to
            variant 1. It encodes isoform 2, which has a shorter and distinct
            C-terminus, compared to isoform 1. Variants 2 and 8-14 all encode
            the same isoform (2).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.3185127.1,
                                           SRR14038193.71669.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000456318.6/ ENSP00000392913.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-437               AC078797.20        103075-103511
            438-1130            AC078797.20        104238-104930
            1131-1214           AC078797.20        106331-106414
            1215-1308           AC078797.20        106498-106591
            1309-1392           AC078797.20        108082-108165
            1393-1807           AC078797.20        109542-109956
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q27.1"
     gene            1..1807
                     /gene="POLR2H"
                     /gene_synonym="RPABC3; RPB17; RPB8"
                     /note="RNA polymerase II, I and III subunit H"
                     /db_xref="GeneID:5437"
                     /db_xref="HGNC:HGNC:9195"
                     /db_xref="MIM:606023"
     exon            1..437
                     /gene="POLR2H"
                     /gene_synonym="RPABC3; RPB17; RPB8"
                     /inference="alignment:Splign:2.1.0"
     exon            438..1130
                     /gene="POLR2H"
                     /gene_synonym="RPABC3; RPB17; RPB8"
                     /inference="alignment:Splign:2.1.0"
     CDS             1058..1510
                     /gene="POLR2H"
                     /gene_synonym="RPABC3; RPB17; RPB8"
                     /EC_number="2.7.7.6"
                     /note="isoform 2 is encoded by transcript variant 2;
                     DNA-directed RNA polymerases I, II, and III subunit
                     RPABC3; RPB8 homolog; DNA-directed RNA polymerase II
                     subunit H; DNA-directed RNA polymerases I, II, and III
                     17.1 kDa polypeptide; polymerase (RNA) II (DNA directed)
                     polypeptide H; polymerase (RNA) II subunit H"
                     /codon_start=1
                     /product="DNA-directed RNA polymerases I, II, and III
                     subunit RPABC3 isoform 2"
                     /protein_id="NP_006223.2"
                     /db_xref="CCDS:CCDS3264.1"
                     /db_xref="GeneID:5437"
                     /db_xref="HGNC:HGNC:9195"
                     /db_xref="MIM:606023"
                     /translation="MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVN
                     IQIYPVDLGDKFRLVIASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRIEGD
                     ETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF"
     exon            1131..1214
                     /gene="POLR2H"
                     /gene_synonym="RPABC3; RPB17; RPB8"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1308
                     /gene="POLR2H"
                     /gene_synonym="RPABC3; RPB17; RPB8"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1392
                     /gene="POLR2H"
                     /gene_synonym="RPABC3; RPB17; RPB8"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1807
                     /gene="POLR2H"
                     /gene_synonym="RPABC3; RPB17; RPB8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1734..1739
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR2H"
                     /gene_synonym="RPABC3; RPB17; RPB8"
                     /note="hexamer: ATTAAA"
     polyA_site      1764
                     /gene="POLR2H"
                     /gene_synonym="RPABC3; RPB17; RPB8"
     polyA_site      1807
                     /gene="POLR2H"
                     /gene_synonym="RPABC3; RPB17; RPB8"
                     /note="major polyA site"
ORIGIN      
        1 atccggaggg gacggcccag ccaggctggg gtccgcgcgg ggcctcccga gaggcggcaa
       61 ggggcgctgg gaaggcgcaa gcccgcgccg gctccctgcg gatgcccggg tcggagaggg
      121 gcgcccggga ggggcgggtc tactggcacg tggaggggcc ggcccgggtt cgcggttccc
      181 cgtgcccagg caggaggggc tgcagacgcc tgagctccgt aggggttggg gctgggggct
      241 gcggagctgt ctacatcgga ctcctccggg tcagcccgtt aggagccgcc tcccctctgc
      301 ccctccgaag agacgagcgg cccagacagg cctgggaagg cccctctgcc ccgtcagggg
      361 tgaaaagcaa agctggaagg attcggagag ggttggggcc gtcttcctca tccttccttt
      421 tctcggggct cccgtggcga cgtcatggca aattcccaaa ctggacggat ccggctcggg
      481 ttacgtccag cagggccgag ccggcccagg ccggccccgg ggtcctcggt gccggctgga
      541 ggggctgggc acctctgggg cggggttctg ctccacctgc cctcggcgcg gactgtgccc
      601 tcggcgggca cgcggaccgg cggggtaggg ctagggcccg gcttctctgg gccaaggatc
      661 aaggtccctg cctgggttag gcccccacgc attccagtct tcggaacccg gcctctgaag
      721 agggcgcagt agggccccag cggagcgcga gaacccgctc tacagcctat tgcttccccg
      781 ccctgggcag agccacctgg acatgagacc cgccctcaat gccgaagcct ctcggaagca
      841 atctttcggg acggaagtta agtagccccg agcgggaggc tgtggcggaa gtggtcgcgt
      901 taccgcttgt ttgtgcgcat gcgccactct cgtctggccg ccgcgctttc aggaggtgct
      961 tttggttctc tccggtcttg tccacgctag ggggtgcacg tactcccaac tgtggtcgcg
     1021 ctctcacccc ttctgctgct ctcgtggccc cctcgcgatg gcgggcatcc tgtttgagga
     1081 tattttcgat gtgaaggata ttgacccgga gggcaagaag tttgaccgag tgtctcgact
     1141 gcattgtgag agtgaatctt tcaagatgga tctaatctta gatgtaaaca ttcaaattta
     1201 ccctgtagac ttgggtgaca agtttcggtt ggtcatagct agtaccttgt atgaagatgg
     1261 taccctggat gatggtgaat acaaccccac tgatgatagg ccttccaggg ctgaccagtt
     1321 tgagtatgta atgtatggaa aagtgtacag gattgaggga gatgaaactt ctactgaagc
     1381 agcaacacgc ctctctgcgt acgtgtccta tgggggcctg ctcatgaggc tgcaggggga
     1441 tgccaacaac ctgcatggat tcgaggtgga ctccagagtt tatctcctga tgaagaagct
     1501 agccttctga acctcgcctg aagccagcct ctctgccaag tcactcaggt catgggcatt
     1561 gttcaagcct gagtggcagc cgctcttgct cacctgttga ggaagggctg gctcactgtc
     1621 caccgtggcg gcatctttaa ctggcctcca ctcaatggga aactgactcg cctgtgaaag
     1681 acacagtggg agagctgaaa atgaatcaga agctttatgt atatgatttt taaattaaac
     1741 tttacttttt cagactgccc ctcccctttt tgtaaaaagt ccatttactg taaaatcgtt
     1801 ttttcca
//
