LOCUS       NM_021974               2070 bp    mRNA    linear   PRI 13-APR-2024
DEFINITION  Homo sapiens RNA polymerase II, I and III subunit F (POLR2F),
            transcript variant 1, mRNA.
ACCESSION   NM_021974
VERSION     NM_021974.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Daiss,J.L., Pilsl,M., Straub,K., Bleckmann,A., Hocherl,M.,
            Heiss,F.B., Abascal-Palacios,G., Ramsay,E.P., Tluckova,K.,
            Mars,J.C., Furtges,T., Bruckmann,A., Rudack,T., Bernecky,C.,
            Lamour,V., Panov,K., Vannini,A., Moss,T. and Engel,C.
  TITLE     The human RNA polymerase I structure reveals an HMG-like docking
            domain specific to metazoans
  JOURNAL   Life Sci Alliance 5 (11), e202201568 (2022)
   PUBMED   36271492
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Okuda,M., Suwa,T., Suzuki,H., Yamaguchi,Y. and Nishimura,Y.
  TITLE     Three human RNA polymerases interact with TFIIH via a common RPB6
            subunit
  JOURNAL   Nucleic Acids Res 50 (1), 1-16 (2022)
   PUBMED   34268577
  REMARK    GeneRIF: Three human RNA polymerases interact with TFIIH via a
            common RPB6 subunit.
REFERENCE   3  (bases 1 to 2070)
  AUTHORS   Hou,H., Li,Y., Wang,M., Liu,A., Yu,Z., Chen,K., Zhao,D. and Xu,Y.
  TITLE     Structural insights into RNA polymerase III-mediated transcription
            termination through trapping poly-deoxythymidine
  JOURNAL   Nat Commun 12 (1), 6135 (2021)
   PUBMED   34675218
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2070)
  AUTHORS   Zhao,D., Liu,W., Chen,K., Wu,Z., Yang,H. and Xu,Y.
  TITLE     Structure of the human RNA polymerase I elongation complex
  JOURNAL   Cell Discov 7 (1), 97 (2021)
   PUBMED   34671025
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2070)
  AUTHORS   Pusch,C., Wang,Z., Roe,B. and Blin,N.
  TITLE     Genomic structure of the RNA polymerase II small subunit (hRPB14.4)
            locus (POLRF) and mapping to 22q13.1 by sequence identity
  JOURNAL   Genomics 34 (3), 440-442 (1996)
   PUBMED   8786150
REFERENCE   6  (bases 1 to 2070)
  AUTHORS   Acker,J., Wintzerith,M., Vigneron,M. and Kedinger,C.
  TITLE     A 14.4 KDa acidic subunit of human RNA polymerase II with a
            putative leucine-zipper
  JOURNAL   DNA Seq 4 (5), 329-331 (1994)
   PUBMED   7803819
REFERENCE   7  (bases 1 to 2070)
  AUTHORS   Kato,H., Sumimoto,H., Pognonec,P., Chen,C.H., Rosen,C.A. and
            Roeder,R.G.
  TITLE     HIV-1 Tat acts as a processivity factor in vitro in conjunction
            with cellular elongation factors
  JOURNAL   Genes Dev 6 (4), 655-666 (1992)
   PUBMED   1559613
REFERENCE   8  (bases 1 to 2070)
  AUTHORS   Jacob,G.A., Luse,S.W. and Luse,D.S.
  TITLE     Abortive initiation is increased only for the weakest members of a
            set of down mutants of the adenovirus 2 major late promoter
  JOURNAL   J Biol Chem 266 (33), 22537-22544 (1991)
   PUBMED   1939271
REFERENCE   9  (bases 1 to 2070)
  AUTHORS   Southgate,C., Zapp,M.L. and Green,M.R.
  TITLE     Activation of transcription by HIV-1 Tat protein tethered to
            nascent RNA through another protein
  JOURNAL   Nature 345 (6276), 640-642 (1990)
   PUBMED   2190099
REFERENCE   10 (bases 1 to 2070)
  AUTHORS   Conaway,R.C. and Conaway,J.W.
  TITLE     ATP activates transcription initiation from promoters by RNA
            polymerase II in a reversible step prior to RNA synthesis
  JOURNAL   J Biol Chem 263 (6), 2962-2968 (1988)
   PUBMED   2449431
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA372532.1 and AL031587.3.
            
            On Nov 22, 2018 this sequence version replaced NM_021974.4.
            
            Summary: This gene encodes the sixth largest subunit of RNA
            polymerase II, the polymerase responsible for synthesizing
            messenger RNA in eukaryotes. In yeast, this polymerase subunit, in
            combination with at least two other subunits, forms a structure
            that stabilizes the transcribing polymerase on the DNA template.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no quality transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.493439.1,
                                           SRR3476690.250556.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000442738.7/ ENSP00000403852.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-582               DA372532.1         37-618
            583-2070            AL031587.3         25103-26590
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.1"
     gene            1..2070
                     /gene="POLR2F"
                     /gene_synonym="HRBP14.4; POLRF; RPABC14.4; RPABC2;
                     RPB14.4; RPB6; RPC15"
                     /note="RNA polymerase II, I and III subunit F"
                     /db_xref="GeneID:5435"
                     /db_xref="HGNC:HGNC:9193"
                     /db_xref="MIM:604414"
     exon            1..109
                     /gene="POLR2F"
                     /gene_synonym="HRBP14.4; POLRF; RPABC14.4; RPABC2;
                     RPB14.4; RPB6; RPC15"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..473
                     /gene="POLR2F"
                     /gene_synonym="HRBP14.4; POLRF; RPABC14.4; RPABC2;
                     RPB14.4; RPB6; RPC15"
                     /EC_number="2.7.7.6"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DNA-directed RNA polymerases I, II, and III subunit
                     RPABC2; RNA Polymerase II subunit 14.4 kD; DNA-directed
                     RNA polymerase II subunit F; RNA polymerases I, II, and
                     III subunit ABC2; DNA-directed RNA polymerases I, II, and
                     III 14.4 kDa polypeptide; polymerase (RNA) II (DNA
                     directed) polypeptide F; polymerase (RNA) II subunit F"
                     /codon_start=1
                     /product="DNA-directed RNA polymerases I, II, and III
                     subunit RPABC2 isoform 1"
                     /protein_id="NP_068809.1"
                     /db_xref="CCDS:CCDS13963.1"
                     /db_xref="GeneID:5435"
                     /db_xref="HGNC:HGNC:9193"
                     /db_xref="MIM:604414"
                     /translation="MSDNEDNFDGDDFDDVEEDEGLDDLENAEEEGQENVEILPSGER
                     PQANQKRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKI
                     PIIIRRYLPDGSYEDWGVDELIITD"
     exon            110..179
                     /gene="POLR2F"
                     /gene_synonym="HRBP14.4; POLRF; RPABC14.4; RPABC2;
                     RPB14.4; RPB6; RPC15"
                     /inference="alignment:Splign:2.1.0"
     exon            180..310
                     /gene="POLR2F"
                     /gene_synonym="HRBP14.4; POLRF; RPABC14.4; RPABC2;
                     RPB14.4; RPB6; RPC15"
                     /inference="alignment:Splign:2.1.0"
     exon            311..382
                     /gene="POLR2F"
                     /gene_synonym="HRBP14.4; POLRF; RPABC14.4; RPABC2;
                     RPB14.4; RPB6; RPC15"
                     /inference="alignment:Splign:2.1.0"
     exon            383..2070
                     /gene="POLR2F"
                     /gene_synonym="HRBP14.4; POLRF; RPABC14.4; RPABC2;
                     RPB14.4; RPB6; RPC15"
                     /inference="alignment:Splign:2.1.0"
     regulatory      538..543
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR2F"
                     /gene_synonym="HRBP14.4; POLRF; RPABC14.4; RPABC2;
                     RPB14.4; RPB6; RPC15"
                     /note="hexamer: AATAAA"
     polyA_site      559
                     /gene="POLR2F"
                     /gene_synonym="HRBP14.4; POLRF; RPABC14.4; RPABC2;
                     RPB14.4; RPB6; RPC15"
                     /note="major polyA site"
     regulatory      2050..2055
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR2F"
                     /gene_synonym="HRBP14.4; POLRF; RPABC14.4; RPABC2;
                     RPB14.4; RPB6; RPC15"
                     /note="hexamer: AATAAA"
     polyA_site      2070
                     /gene="POLR2F"
                     /gene_synonym="HRBP14.4; POLRF; RPABC14.4; RPABC2;
                     RPB14.4; RPB6; RPC15"
ORIGIN      
        1 aagctaggag ccgcgcgagt cgtagtgtcg ctgtttgcgg gtctccgcgc gggaccgggg
       61 cgcagcgggg tcgctgaggc gagggtgtca tgtcagacaa cgaggacaat tttgatggcg
      121 acgactttga tgatgtggag gaggatgaag ggctagatga cttggagaat gccgaagagg
      181 aaggccagga gaatgtcgag atcctcccct ctggggagcg accgcaggcc aaccagaagc
      241 gaatcaccac accatacatg accaagtacg agcgagcccg cgtgctgggc acccgagcgc
      301 tccagattgc gatgtgtgcc cctgtgatgg tggagctgga gggggagaca gatcctctgc
      361 tcattgccat gaaggaactc aaggcccgaa agatccccat catcattcgc cgttacctgc
      421 cagatgggag ctatgaagac tggggggtgg acgagctcat catcaccgac tgagctggag
      481 tcatcttcct gcccttgccc catgcccaat tttcattctc actttatatg tgtaaataat
      541 aaaatattca actttccaac ccccttcccc tctgcttatc tgcaatgtca ccacctgttg
      601 cttccccgtt accgccatgc tgcgtggagc atgcacctat tccagtggcc ctgtgactgt
      661 cagctcctta agaagcacca ggggccctta gcccctttgg atcccccaca tccttccctc
      721 catctccctg ttccccagag caaaggctgc tgcaggggag acacctcagc tgccttccaa
      781 gcagacagac aagtctttgt gcccagggag ctggttgcca cggaaacccc caatttcctt
      841 tccagtgggg actggctgca ggggcttctc ccttctcagg agtatcacag agcaggtctc
      901 atcaagccac ccattgtttc ctaaggacct gcttcgagcc tcttatcgtg ggctcggatc
      961 ccctttcagg agcagtgccc cagcaggaag cgtgggggtg tgctgatctc ccaccctccc
     1021 caggcagagc cctgctgggc aagtcagcag ctggagcaag gaccgagcac ctgcctaccc
     1081 ctgcccccat ggctctgtcc ccactcctcc tcaggactct gcccacacgc tgtcctctgt
     1141 ggcccacctc tttggtgtgc cacacccatg ccttcttcct agcctctata agatatcctt
     1201 tccctcctat ttggggctgg tgatcccctg aggccttggg gacatggtgc tggggtggtg
     1261 gtgctcctgg gttccaggtg ttacattaag tcagagctgg agttccccct tcctctgcct
     1321 gaggttctaa tgggtcctct tcctttctcc accctgctta cccaacctga ggtaagacca
     1381 gtcacactgg ctcctccctc ctagaggggg tcagggggag ggtgtatatt gacatgaaca
     1441 gggatagagg gtaaactggc tccctgaata tgccagcctt aacctccatt ccactgccag
     1501 ctccccttca aagaggagga gctgggcttc cctaacctct gcaggaggca gggcctccag
     1561 gcctaggtgc agcctggccc tgggatggga tgtggggagt gaatggtgag gatctgcatt
     1621 ggtgggaggg gtgtccgctg ccctggagaa gggttaattc agggagcagt ggacttcaca
     1681 ctcccatcca ccctcctcca agcctgtgga atcctttaat caagttgggt gctgaaattt
     1741 cagctctgaa atgccgcctt tgtgctggca tccaggcagc cgccccagag tgcgggggtc
     1801 actttctcgg ccccatttct ctaaatggtc tctttgttcc ctgctgggct gctcagtatc
     1861 agatgtgatt aaagggagat ggggtttggg ctggggagga ggggaattgg gggttaaccc
     1921 ttaggggata gagtgcaagg gaaatgggac agaaggggtg tttttcctcc tgtcttcctc
     1981 ttcccattct cctcttttgg ggagtcccct gctattcagc tgtctgggcc tggcttttga
     2041 cttctcttga ataaaatgtc ccggtcacca
//
