LOCUS       NM_002695               2854 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens RNA polymerase II, I and III subunit E (POLR2E),
            transcript variant 1, mRNA.
ACCESSION   NM_002695
VERSION     NM_002695.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Daiss,J.L., Pilsl,M., Straub,K., Bleckmann,A., Hocherl,M.,
            Heiss,F.B., Abascal-Palacios,G., Ramsay,E.P., Tluckova,K.,
            Mars,J.C., Furtges,T., Bruckmann,A., Rudack,T., Bernecky,C.,
            Lamour,V., Panov,K., Vannini,A., Moss,T. and Engel,C.
  TITLE     The human RNA polymerase I structure reveals an HMG-like docking
            domain specific to metazoans
  JOURNAL   Life Sci Alliance 5 (11), e202201568 (2022)
   PUBMED   36271492
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 2854)
  AUTHORS   Hou,H., Li,Y., Wang,M., Liu,A., Yu,Z., Chen,K., Zhao,D. and Xu,Y.
  TITLE     Structural insights into RNA polymerase III-mediated transcription
            termination through trapping poly-deoxythymidine
  JOURNAL   Nat Commun 12 (1), 6135 (2021)
   PUBMED   34675218
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2854)
  AUTHORS   Zhao,D., Liu,W., Chen,K., Wu,Z., Yang,H. and Xu,Y.
  TITLE     Structure of the human RNA polymerase I elongation complex
  JOURNAL   Cell Discov 7 (1), 97 (2021)
   PUBMED   34671025
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2854)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 2854)
  AUTHORS   Kato,H., Sumimoto,H., Pognonec,P., Chen,C.H., Rosen,C.A. and
            Roeder,R.G.
  TITLE     HIV-1 Tat acts as a processivity factor in vitro in conjunction
            with cellular elongation factors
  JOURNAL   Genes Dev 6 (4), 655-666 (1992)
   PUBMED   1559613
REFERENCE   7  (bases 1 to 2854)
  AUTHORS   Jacob,G.A., Luse,S.W. and Luse,D.S.
  TITLE     Abortive initiation is increased only for the weakest members of a
            set of down mutants of the adenovirus 2 major late promoter
  JOURNAL   J Biol Chem 266 (33), 22537-22544 (1991)
   PUBMED   1939271
REFERENCE   8  (bases 1 to 2854)
  AUTHORS   Pati,U.K. and Weissman,S.M.
  TITLE     Isolation and molecular characterization of a cDNA encoding the
            23-kDa subunit of human RNA polymerase II
  JOURNAL   J Biol Chem 266 (20), 13468 (1991)
   PUBMED   2071613
  REMARK    Correction to:[J Biol Chem. 1989 Aug 5;264(22):13114-21.. PMID:
            2753903]
REFERENCE   9  (bases 1 to 2854)
  AUTHORS   Southgate,C., Zapp,M.L. and Green,M.R.
  TITLE     Activation of transcription by HIV-1 Tat protein tethered to
            nascent RNA through another protein
  JOURNAL   Nature 345 (6276), 640-642 (1990)
   PUBMED   2190099
REFERENCE   10 (bases 1 to 2854)
  AUTHORS   Conaway,R.C. and Conaway,J.W.
  TITLE     ATP activates transcription initiation from promoters by RNA
            polymerase II in a reversible step prior to RNA synthesis
  JOURNAL   J Biol Chem 263 (6), 2962-2968 (1988)
   PUBMED   2449431
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC004151.1.
            
            On May 21, 2020 this sequence version replaced NM_002695.4.
            
            Summary: This gene encodes the fifth largest subunit of RNA
            polymerase II, the polymerase responsible for synthesizing
            messenger RNA in eukaryotes. This subunit is shared by the other
            two DNA-directed RNA polymerases and is present in two-fold molar
            excess over the other polymerase subunits. An interaction between
            this subunit and a hepatitis virus transactivating protein has been
            demonstrated, suggesting that interaction between transcriptional
            activators and the polymerase can occur through this subunit. A
            pseudogene is located on chromosome 11. Three transcript variants
            encoding two different isoforms have been found for this gene.
            [provided by RefSeq, Oct 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853566.13091.1,
                                           SRR11853563.16058.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000615234.5/ ENSP00000478303.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-117               AC004151.1         22962-23078         c
            118-292             AC004151.1         21607-21781         c
            293-408             AC004151.1         19495-19610         c
            409-489             AC004151.1         18611-18691         c
            490-548             AC004151.1         17790-17848         c
            549-627             AC004151.1         17587-17665         c
            628-707             AC004151.1         17175-17254         c
            708-2854            AC004151.1         14277-16423         c
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..2854
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
                     /note="RNA polymerase II, I and III subunit E"
                     /db_xref="GeneID:5434"
                     /db_xref="HGNC:HGNC:9192"
                     /db_xref="MIM:180664"
     exon            1..117
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..693
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
                     /EC_number="2.7.7.6"
                     /note="isoform a is encoded by transcript variant 1; DNA
                     directed RNA polymerase II 23 kda polypeptide; polymerase
                     (RNA) II (DNA directed) polypeptide E, 25kDa; DNA-directed
                     RNA polymerases I, II, and III subunit RPABC1; RPB5
                     homolog; DNA-directed RNA polymerase II subunit E; RNA
                     polymerases I, II, and III subunit ABC1; DNA-directed RNA
                     polymerase II 23 kDa polypeptide; DNA-directed RNA
                     polymerase subunit RPABC1; polymerase (RNA) II subunit E"
                     /codon_start=1
                     /product="DNA-directed RNA polymerases I, II, and III
                     subunit RPABC1 isoform a"
                     /protein_id="NP_002686.3"
                     /db_xref="CCDS:CCDS12056.1"
                     /db_xref="GeneID:5434"
                     /db_xref="HGNC:HGNC:9192"
                     /db_xref="MIM:180664"
                     /translation="MDDEEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQS
                     GDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRA
                     LIVVQQGMTPSAKQSLVDMAPKYILEQFLQQELLINITEHELVPEHVVMTKEEVTELL
                     ARYKLRENQLPRIQAGDPVARYFGIKRGQVVKIIRPSETAGRYITYRLVQ"
     exon            118..292
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            293..408
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            409..489
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            490..548
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            549..627
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            628..707
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            708..2854
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1242..1247
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
                     /note="hexamer: AATAAA"
     polyA_site      1264
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
                     /note="major polyA site"
     regulatory      2804..2809
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
                     /note="hexamer: ATTAAA"
     regulatory      2828..2833
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
                     /note="hexamer: AATAAA"
     polyA_site      2829
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
     polyA_site      2854
                     /gene="POLR2E"
                     /gene_synonym="hRPB25; hsRPB5; RPABC1; RPB5; XAP4"
ORIGIN      
        1 agtccgcgcg ggttctcgcg cggagaaggg gtgcgagcgg cggcggcggc ggaggctgcc
       61 atggacgacg aggaggagac gtaccggctc tggaaaatcc gcaagaccat catgcagctg
      121 tgccacgacc gtggctatct ggtgacccag gacgagcttg accagaccct ggaggagttc
      181 aaagcccaat ctggggacaa gccgagtgag gggcggccgc ggcgcacgga cctcaccgtg
      241 ctggtggccc acaacgatga ccccaccgac cagatgtttg tgttctttcc agaggagccc
      301 aaggtgggca tcaagaccat caaggtgtac tgccagcgca tgcaggagga gaacatcaca
      361 cgggctctca tcgtggtgca gcagggcatg acaccctccg ccaagcagtc cctggtcgac
      421 atggccccca agtacatcct ggagcagttt ctgcagcagg agctgctcat caacatcacg
      481 gagcacgagc tagtccctga gcacgtcgtc atgaccaagg aggaggtgac agagctgctg
      541 gcccgatata agctccgaga gaaccagctg cccaggatcc aggcggggga ccctgtggcg
      601 cgctactttg ggataaagcg tgggcaggtg gtgaagatca tccggcccag tgagacggct
      661 ggcaggtaca tcacctaccg gctggtgcag tagctaccgc ctgacagccc ctagaggcgg
      721 acacacagcg acccccatcc ctgcaggaca aacgcccctg ccctgccaga atccggcccc
      781 cacagctctc acggctgctg ctcctctgga ctccccaagg caggtggcct ccacccacgt
      841 tctcccgtcc tggggtgagg cttcctgtgg cccagcccgc cccattcacc tgtggatttg
      901 tgcgagatgc agcctcagaa ggaacaaggc ccccagaggg aggtcacctg ggggcagctg
      961 gtgccgggtc ttcacccaga ccacgctggg tcccctctgt tgggggtttg gggtccgggt
     1021 ctcccaccag ccactgcttc ctcctgggcc ctcggccttc cacccctcgt cttccctccc
     1081 tcgggggccc tgatgcgtgg cggcccccgc ccggcctcgg ctctttactc cattcacagc
     1141 catgcacgcg ctcaagccac cagggtgcga gatgccagct ctggagttct cggttgttgt
     1201 aggaggttgg gtgttttcaa atggtaaaga tgttttgagc aaataaattt gcttgataca
     1261 gaaaccaggc cgactaacaa ggtttccagc aggtggcctt gcctcacccc aagcacacga
     1321 ggtcagcctg gcaggtgtgc cctcctcgcg ctggcttcag gggtcagtgt ctccccaggc
     1381 cctcgccctg ccctcccact tcgtgctcct gggtaaggcg tgagcgtgtc tgtctcttcc
     1441 cttcccgtga gggtgtggag ttgttctctg gcttgtgcag ctgtggtttg cggccagttc
     1501 ctcttacaga cgtttgggat ctggctctac ttccccgttt gcttactgtc tgatttcaga
     1561 aggcctcgcc tgcccaagtt taacctagcc acgtggttct ctcacagtag ctttattaag
     1621 atgtaattca ccctcgggcg gtcggtggtc tgtaacgcgc ccactgttct gtgcagcagg
     1681 gtccccagat ccccaggccg cggactgtgt gtgtgatggg gacagtgctt ccccttctct
     1741 ccaaatgtac ttttttcaaa attgtcttgg ccaaagcaag ccagacccaa agggacaaac
     1801 actgatgtca ctcctaggag gtcactagag tcgtcagaga ctggaagtag gatgggggtt
     1861 cccgggactg gggagttggg tggggatggg ggttcccagg gctggggagg agggagggga
     1921 cagagtgtgt catgtgcaca gttttagttt gggaagatga gaaaattctg gaggtggccg
     1981 ggcgcggtgg ctcacgcctg taatcccagc actttgggag gccgaggtgg gcggatcacc
     2041 tgaggtcggg agttcgagac cagcctgacc aacatggcaa aatcccatca ctgcaaaaaa
     2101 tacaaaaatt acctgggcgt ggtggtgcat gcctgtaatc ccagctactt gggagctaag
     2161 gcaggagaat cacttgaacc caggaagtgg aggatcgctt gagctcagga gttaaaagac
     2221 cagcccgggc aacacagaga aaccccccac ctctacaaaa aactttaaga ttacctgggc
     2281 acagtggctc acacctgtaa tcccaacact ttgggaggct gaggcgggag gatcatttga
     2341 gcccaggagg ccgaggctgc agtgagctac gattgtgctc ctgtactcca gcctgggcca
     2401 cagggtcaca ccctgtctca aaaaaaaaaa aaaatgcagt tccacaggcg catgggccac
     2461 gtttccagtg cttggtggcc atgtggggct gacgtgcgaa accttagagc cgggggatac
     2521 gtccgtcatc gagaacgttt tcctggactg ccctgggctg caggtggacg tgacgaggtc
     2581 attcgatgcc tgaccgtctg cacagcctgt ggtgaagacc acccgggctc ccacctcgcg
     2641 gggccccgtg gcggggggtt cccttcctca cacatccctc ccctaggggg aggcccaggg
     2701 ccacgccacg gggctctctc tgtcccctcc ctggcccctt cttctaagct tgcagctgcc
     2761 acagaaaatc ttctaagctt gcagctgcca cagaaaacac ccgattaaaa actttttatt
     2821 tcagcaaaat aaacgtgcct gtgaaagaac caca
//
