LOCUS       NM_032940               1764 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens RNA polymerase II subunit C (POLR2C), mRNA.
ACCESSION   NM_032940
VERSION     NM_032940.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Burke,K.A., Janke,A.M., Rhine,C.L. and Fawzi,N.L.
  TITLE     Residue-by-Residue View of In Vitro FUS Granules that Bind the
            C-Terminal Domain of RNA Polymerase II
  JOURNAL   Mol Cell 60 (2), 231-241 (2015)
   PUBMED   26455390
  REMARK    GeneRIF: FUS low-complexicity phase separates to recruit RNA
            polymerase II C-terminal domain in vitro.
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Fang,Z.P., Jiang,B.G., Zhang,F.B., Wang,A.D., Ji,Y.M., Xu,Y.F.,
            Li,J.C., Zhou,W.P., Zhou,W.J. and Han,H.X.
  TITLE     Rpb3 promotes hepatocellular carcinoma through its N-terminus
  JOURNAL   Oncotarget 5 (19), 9256-9268 (2014)
   PUBMED   25211001
  REMARK    GeneRIF: These findings suggest that N-terminus of Rpb3 selectively
            inhibits Rpb3-high-expression hepatocellular carcinoma cells
            proliferation. N-terminus of Rpb3 may be useful in treating
            patients diagnosed with Rpb3-high-expression hepatocellular
            carcinoma.
REFERENCE   3  (bases 1 to 1764)
  AUTHORS   Corbi,N., Batassa,E.M., Pisani,C., Onori,A., Di Certo,M.G.,
            Strimpakos,G., Fanciulli,M., Mattei,E. and Passananti,C.
  TITLE     The eEF1gamma subunit contacts RNA polymerase II and binds vimentin
            promoter region
  JOURNAL   PLoS One 5 (12), e14481 (2010)
   PUBMED   21217813
  REMARK    GeneRIF: eEF1gamma, in addition to its role in translation
            elongation complex, is involved in regulating Vimentin gene by
            contacting both pol II and the Vimentin promoter region and
            shuttling/nursing the Vimentin mRNA
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1764)
  AUTHORS   Michiels,S., Laplanche,A., Boulet,T., Dessen,P., Guillonneau,B.,
            Mejean,A., Desgrandchamps,F., Lathrop,M., Sarasin,A. and
            Benhamou,S.
  TITLE     Genetic polymorphisms in 85 DNA repair genes and bladder cancer
            risk
  JOURNAL   Carcinogenesis 30 (5), 763-768 (2009)
   PUBMED   19237606
  REMARK    GeneRIF: Observational study of gene-disease association, gene-gene
            interaction, and gene-environment interaction. (HuGE Navigator)
REFERENCE   5  (bases 1 to 1764)
  AUTHORS   Oufattole,M., Lin,S.W., Liu,B., Mascarenhas,D., Cohen,P. and
            Rodgers,B.D.
  TITLE     Ribonucleic acid polymerase II binding subunit 3 (Rpb3), a
            potential nuclear target of insulin-like growth factor binding
            protein-3
  JOURNAL   Endocrinology 147 (5), 2138-2146 (2006)
   PUBMED   16455777
  REMARK    GeneRIF: Rpb3 is a potential nuclear target of insulin-like growth
            factor binding protein-3
REFERENCE   6  (bases 1 to 1764)
  AUTHORS   Kato,H., Sumimoto,H., Pognonec,P., Chen,C.H., Rosen,C.A. and
            Roeder,R.G.
  TITLE     HIV-1 Tat acts as a processivity factor in vitro in conjunction
            with cellular elongation factors
  JOURNAL   Genes Dev 6 (4), 655-666 (1992)
   PUBMED   1559613
REFERENCE   7  (bases 1 to 1764)
  AUTHORS   Jacob,G.A., Luse,S.W. and Luse,D.S.
  TITLE     Abortive initiation is increased only for the weakest members of a
            set of down mutants of the adenovirus 2 major late promoter
  JOURNAL   J Biol Chem 266 (33), 22537-22544 (1991)
   PUBMED   1939271
REFERENCE   8  (bases 1 to 1764)
  AUTHORS   Southgate,C., Zapp,M.L. and Green,M.R.
  TITLE     Activation of transcription by HIV-1 Tat protein tethered to
            nascent RNA through another protein
  JOURNAL   Nature 345 (6276), 640-642 (1990)
   PUBMED   2190099
REFERENCE   9  (bases 1 to 1764)
  AUTHORS   Pati,U.K. and Weissman,S.M.
  TITLE     The amino acid sequence of the human RNA polymerase II 33-kDa
            subunit hRPB 33 is highly conserved among eukaryotes
  JOURNAL   J Biol Chem 265 (15), 8400-8403 (1990)
   PUBMED   2187864
REFERENCE   10 (bases 1 to 1764)
  AUTHORS   Rubinstein,M.H.
  TITLE     A new granulation method for compressed tablets [proceedings]
  JOURNAL   J Pharm Pharmacol 28 Suppl, 67P (1976)
   PUBMED   12345
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI458170.1 and BC003159.1.
            
            On Nov 23, 2018 this sequence version replaced NM_032940.2.
            
            Summary: This gene encodes the third largest subunit of RNA
            polymerase II, the polymerase responsible for synthesizing
            messenger RNA in eukaryotes. The product of this gene contains a
            cysteine rich region and exists as a heterodimer with another
            polymerase subunit, POLR2J. These two subunits form a core
            subassembly unit of the polymerase. A pseudogene has been
            identified on chromosome 21. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC003159.1, SRR3476690.801283.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000219252.10/ ENSP00000219252.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-503               BI458170.1         49-551
            504-1764            BC003159.1         516-1776
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q21"
     gene            1..1764
                     /gene="POLR2C"
                     /gene_synonym="hRPB33; hsRPB3; RPB3; RPB31"
                     /note="RNA polymerase II subunit C"
                     /db_xref="GeneID:5432"
                     /db_xref="HGNC:HGNC:9189"
                     /db_xref="MIM:180663"
     exon            1..132
                     /gene="POLR2C"
                     /gene_synonym="hRPB33; hsRPB3; RPB3; RPB31"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..874
                     /gene="POLR2C"
                     /gene_synonym="hRPB33; hsRPB3; RPB3; RPB31"
                     /EC_number="2.7.7.6"
                     /note="RNA polymerase II subunit 3; RPB33; polymerase
                     (RNA) II (DNA directed) polypeptide C, 33kDa; RNA
                     polymerase II subunit B3; DNA-directed RNA polymerase II
                     subunit C; polymerase (RNA) II subunit C; DNA-directed RNA
                     polymerase II 33 kDa polypeptide"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase II subunit RPB3"
                     /protein_id="NP_116558.1"
                     /db_xref="CCDS:CCDS10782.1"
                     /db_xref="GeneID:5432"
                     /db_xref="HGNC:HGNC:9189"
                     /db_xref="MIM:180663"
                     /translation="MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPI
                     IAIDWVQIDANSSVLHDEFIAHRLGLIPLISDDIVDKLQYSRDCTCEEFCPECSVEFT
                     LDVRCNEDQTRHVTSRDLISNSPRVIPVTSRNRDNDPNDYVEQDDILIVKLRKGQELR
                     LRAYAKKGFGKEHAKWNPTAGVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQA
                     PYDPNGKPERFYYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN"
     exon            133..182
                     /gene="POLR2C"
                     /gene_synonym="hRPB33; hsRPB3; RPB3; RPB31"
                     /inference="alignment:Splign:2.1.0"
     exon            183..251
                     /gene="POLR2C"
                     /gene_synonym="hRPB33; hsRPB3; RPB3; RPB31"
                     /inference="alignment:Splign:2.1.0"
     exon            252..304
                     /gene="POLR2C"
                     /gene_synonym="hRPB33; hsRPB3; RPB3; RPB31"
                     /inference="alignment:Splign:2.1.0"
     exon            305..433
                     /gene="POLR2C"
                     /gene_synonym="hRPB33; hsRPB3; RPB3; RPB31"
                     /inference="alignment:Splign:2.1.0"
     exon            434..485
                     /gene="POLR2C"
                     /gene_synonym="hRPB33; hsRPB3; RPB3; RPB31"
                     /inference="alignment:Splign:2.1.0"
     exon            486..654
                     /gene="POLR2C"
                     /gene_synonym="hRPB33; hsRPB3; RPB3; RPB31"
                     /inference="alignment:Splign:2.1.0"
     exon            655..729
                     /gene="POLR2C"
                     /gene_synonym="hRPB33; hsRPB3; RPB3; RPB31"
                     /inference="alignment:Splign:2.1.0"
     exon            730..1764
                     /gene="POLR2C"
                     /gene_synonym="hRPB33; hsRPB3; RPB3; RPB31"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1417..1422
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR2C"
                     /gene_synonym="hRPB33; hsRPB3; RPB3; RPB31"
                     /note="hexamer: AATAAA"
     polyA_site      1439
                     /gene="POLR2C"
                     /gene_synonym="hRPB33; hsRPB3; RPB3; RPB31"
                     /note="major polyA site"
     regulatory      1742..1747
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR2C"
                     /gene_synonym="hRPB33; hsRPB3; RPB3; RPB31"
                     /note="hexamer: AATAAA"
     polyA_site      1764
                     /gene="POLR2C"
                     /gene_synonym="hRPB33; hsRPB3; RPB3; RPB31"
                     /note="major polyA site"
ORIGIN      
        1 gcagtcaccg cggagcagac gcggaggctg gtggcccctg ggcgagatgc cgtacgccaa
       61 ccagcctacc gtgcggatca cggagctcac tgacgagaat gtcaagttca tcatcgagaa
      121 caccgacctg gcggtggcca attcgattcg gagggtcttc atcgctgagg ttcccataat
      181 agccattgac tgggttcaga ttgatgccaa ttcctcagtt cttcatgatg aattcattgc
      241 tcacaggctt ggattaattc ccctcattag tgatgacatt gtggacaagc tgcagtactc
      301 tcgggactgc acatgtgagg agttctgccc cgagtgctcg gtggagttca ccctcgatgt
      361 gcggtgcaat gaagaccaga cgcgacatgt cacgtctcga gacctcatct ccaacagccc
      421 ccgggtcatt ccggtgacat cccggaaccg agataatgac cccaatgact acgtggagca
      481 ggatgacatc ctcatcgtca agttgagaaa gggccaggag ctgagacttc gagcctatgc
      541 caaaaagggc tttggcaagg agcatgccaa gtggaaccct actgcagggg tggcttttga
      601 atacgatcca gacaatgccc tgaggcacac agtgtacccc aagcccgagg aatggccaaa
      661 gagtgagtac tcggagctgg atgaggatga gtcgcaggct ccctatgacc ccaacggcaa
      721 gccagaaagg ttttactaca atgtggagtc ctgtggctct ctgcgtcctg aaaccattgt
      781 cctgtcagcc ctctcaggat tgaagaagaa actgagtgat ttacaaactc aattaagcca
      841 cgagatccag agtgatgtgc taaccataaa ttaactgcag cttgcctgct tcagcaaaaa
      901 cggagattca ggccagcagc tggatatggg ggtctctctt cagactcttc tcgtttctga
      961 gaatctagtc tactgttggt tgagcttctt ggcaggacat cagtaccaac tagaagtggg
     1021 tcatagatag attaccaggg atgcagtggt gtttaggcag gataggtctt tactggccct
     1081 gactgctgtt aataattggc agcagtgctc cccagatccc agaaggtccc tgctggagtg
     1141 tttccagtgc acctgtaggg aaccaactag acttctctcc tggttagtcc agctctttac
     1201 tctaaaccct ttctgtccaa aatgagtcat tttcagttgt accttagatg tctggtgttg
     1261 aggatcaagt gccatagcct ttattcaggg ggcctataaa cccttccagt tcttgcccca
     1321 gggctggcct gctagcgctt caaattccca ggtgtcccta atttgagaag taaccttttg
     1381 gaatagcatt agaccctggc tgtcccctcc ccaccaaata aacatgatat ttcattctct
     1441 gtccagcagt catgaacccc ttcacctcca atgacctgat catttagttt ggtgggggtg
     1501 ggggtggggg tgggggtgga agcagccgca ggagcaaggg cccctcccac atacacagga
     1561 ggagtatttc atttctcctt aatgaaggct ctggccctaa cccctcagca ctgtctccag
     1621 ataggaacat gcacaaagca gttaattagg cagcctggag aaaaccagag atccagtaca
     1681 gaaaggaaag gatatttatt gattaacaga agttgtcttt ttaaaagtgt ttatttttgg
     1741 caataaagag cacaacataa tctc
//
