LOCUS       NM_000938               3785 bp    mRNA    linear   PRI 19-AUG-2024
DEFINITION  Homo sapiens RNA polymerase II subunit B (POLR2B), transcript
            variant 1, mRNA.
ACCESSION   NM_000938
VERSION     NM_000938.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Lin,H., Cui,Z., E,T., Xu,H., Wang,D., Wang,P., Ruan,X., Liu,L. and
            Xue,Y.
  TITLE     M6A-methylated circPOLR2B forms an R-loop and regulates the
            biological behavior of glioma stem cells through positive feedback
            loops
  JOURNAL   Cell Death Dis 15 (8), 554 (2024)
   PUBMED   39090090
  REMARK    GeneRIF: M6A-methylated circPOLR2B forms an R-loop and regulates
            the biological behavior of glioma stem cells through positive
            feedback loops.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Li,X.L., Xie,Y., Chen,Y.L., Zhang,Z.M., Tao,Y.F., Li,G., Wu,D.,
            Wang,H.R., Zhuo,R., Pan,J.J., Yu,J.J., Jia,S.Q., Zhang,Z.,
            Feng,C.X., Wang,J.W., Fang,F., Qian,G.H., Lu,J., Hu,S.Y., Li,Z.H.
            and Pan,J.
  TITLE     The RNA polymerase II subunit B (RPB2) functions as a growth
            regulator in human glioblastoma
  JOURNAL   Biochem Biophys Res Commun 674, 170-182 (2023)
   PUBMED   37423037
  REMARK    GeneRIF: The RNA polymerase II subunit B (RPB2) functions as a
            growth regulator in human glioblastoma.
REFERENCE   3  (bases 1 to 3785)
  AUTHORS   Lykke-Andersen,S., Rouviere,J.O. and Jensen,T.H.
  TITLE     ARS2/SRRT: at the nexus of RNA polymerase II transcription,
            transcript maturation and quality control
  JOURNAL   Biochem Soc Trans 49 (3), 1325-1336 (2021)
   PUBMED   34060620
  REMARK    GeneRIF: ARS2/SRRT: at the nexus of RNA polymerase II
            transcription, transcript maturation and quality control.
            Review article
REFERENCE   4  (bases 1 to 3785)
  AUTHORS   He,M., Xu,M., Zhang,B., Liang,J., Chen,P., Lee,J.Y., Johnson,T.A.,
            Li,H., Yang,X., Dai,J., Liang,L., Gui,L., Qi,Q., Huang,J., Li,Y.,
            Adair,L.S., Aung,T., Cai,Q., Cheng,C.Y., Cho,M.C., Cho,Y.S.,
            Chu,M., Cui,B., Gao,Y.T., Go,M.J., Gu,D., Gu,W., Guo,H., Hao,Y.,
            Hong,J., Hu,Z., Hu,Y., Huang,J., Hwang,J.Y., Ikram,M.K., Jin,G.,
            Kang,D.H., Khor,C.C., Kim,B.J., Kim,H.T., Kubo,M., Lee,J., Lee,J.,
            Lee,N.R., Li,R., Li,J., Liu,J., Longe,J., Lu,W., Lu,X., Miao,X.,
            Okada,Y., Ong,R.T., Qiu,G., Seielstad,M., Sim,X., Song,H.,
            Takeuchi,F., Tanaka,T., Taylor,P.R., Wang,L., Wang,W., Wang,Y.,
            Wu,C., Wu,Y., Xiang,Y.B., Yamamoto,K., Yang,H., Liao,M., Yokota,M.,
            Young,T., Zhang,X., Kato,N., Wang,Q.K., Zheng,W., Hu,F.B., Lin,D.,
            Shen,H., Teo,Y.Y., Mo,Z., Wong,T.Y., Lin,X., Mohlke,K.L., Ning,G.,
            Tsunoda,T., Han,B.G., Shu,X.O., Tai,E.S., Wu,T. and Qi,L.
  TITLE     Meta-analysis of genome-wide association studies of adult height in
            East Asians identifies 17 novel loci
  JOURNAL   Hum Mol Genet 24 (6), 1791-1800 (2015)
   PUBMED   25429064
REFERENCE   5  (bases 1 to 3785)
  AUTHORS   Grolimund,L., Aeby,E., Hamelin,R., Armand,F., Chiappe,D.,
            Moniatte,M. and Lingner,J.
  TITLE     A quantitative telomeric chromatin isolation protocol identifies
            different telomeric states
  JOURNAL   Nat Commun 4, 2848 (2013)
   PUBMED   24270157
REFERENCE   6  (bases 1 to 3785)
  AUTHORS   Schaller,S., Grandemange,S., Shpakovski,G.V., Golemis,E.A.,
            Kedinger,C. and Vigneron,M.
  TITLE     Interactions between the full complement of human RNA polymerase II
            subunits
  JOURNAL   FEBS Lett 461 (3), 253-257 (1999)
   PUBMED   10567706
REFERENCE   7  (bases 1 to 3785)
  AUTHORS   Kershnar,E., Wu,S.Y. and Chiang,C.M.
  TITLE     Immunoaffinity purification and functional characterization of
            human transcription factor IIH and RNA polymerase II from clonal
            cell lines that conditionally express epitope-tagged subunits of
            the multiprotein complexes
  JOURNAL   J Biol Chem 273 (51), 34444-34453 (1998)
   PUBMED   9852112
REFERENCE   8  (bases 1 to 3785)
  AUTHORS   Acker,J., de Graaff,M., Cheynel,I., Khazak,V., Kedinger,C. and
            Vigneron,M.
  TITLE     Interactions between the human RNA polymerase II subunits
  JOURNAL   J Biol Chem 272 (27), 16815-16821 (1997)
   PUBMED   9201987
REFERENCE   9  (bases 1 to 3785)
  AUTHORS   Acker,J., Mattei,M.G., Wintzerith,M., Roeckel,N., Depetris,D.,
            Vigneron,M. and Kedinger,C.
  TITLE     Chromosomal localization of human RNA polymerase II subunit genes
  JOURNAL   Genomics 20 (3), 496-499 (1994)
   PUBMED   8034326
REFERENCE   10 (bases 1 to 3785)
  AUTHORS   Acker,J., Wintzerith,M., Vigneron,M. and Kedinger,C.
  TITLE     Primary structure of the second largest subunit of human RNA
            polymerase II (or B)
  JOURNAL   J Mol Biol 226 (4), 1295-1299 (1992)
   PUBMED   1518060
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC023503.2 and AK294901.1.
            
            On Nov 23, 2018 this sequence version replaced NM_000938.2.
            
            Summary: This gene encodes the second largest subunit of RNA
            polymerase II (Pol II), a DNA-dependent RNA polymerase that
            catalyzes the transcription of DNA into precursors of mRNA, snRNA
            and microRNA. This subunit and the largest subunit form opposite
            sides of the center cleft of Pol II. Deletion of the flap loop
            region of this subunit results in a decrease in the rate of
            transcriptional elongation. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Dec 2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.148220.1, BC023503.2
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000314595.6/ ENSP00000312735.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1679              BC023503.2         15-1693
            1680-1938           AK294901.1         1561-1819
            1939-3785           BC023503.2         1953-3799
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q12"
     gene            1..3785
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /note="RNA polymerase II subunit B"
                     /db_xref="GeneID:5431"
                     /db_xref="HGNC:HGNC:9188"
                     /db_xref="MIM:180661"
     exon            1..109
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..3615
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /EC_number="2.7.7.6"
                     /EC_number="2.7.7.48"
                     /note="isoform 1 is encoded by transcript variant 1; RNA
                     polymerase II second largest subunit; DNA-directed RNA
                     polymerase II subunit RPB2; RNA polymerase II subunit 2;
                     RNA polymerase II subunit B2; DNA-directed RNA polymerase
                     II subunit B; DNA-directed RNA polymerase II 140 kDa
                     polypeptide; polymerase (RNA) II (DNA directed)
                     polypeptide B, 140kDa; polymerase (RNA) II subunit B;
                     3'-5' exoribonuclease; RNA-directed RNA polymerase II
                     subunit RPB2"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase II subunit RPB2
                     isoform 1"
                     /protein_id="NP_000929.1"
                     /db_xref="CCDS:CCDS3511.1"
                     /db_xref="GeneID:5431"
                     /db_xref="HGNC:HGNC:9188"
                     /db_xref="MIM:180661"
                     /translation="MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQL
                     DSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERD
                     GAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTY
                     CLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYT
                     GECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRAL
                     GFVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEK
                     RIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKR
                     LDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLAT
                     GNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGM
                     VCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATK
                     IFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRP
                     LLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDL
                     QEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNF
                     HVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNR
                     SAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAP
                     GVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLNQE
                     GYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAI
                     PSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYN
                     GFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGE
                     MERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGCRNKTQI
                     SLVRMPYACKLLFQELMSMSIAPRMMSV"
     exon            110..182
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            183..333
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            334..446
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            447..666
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            667..825
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            826..990
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1187
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1307
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1308..1494
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1638
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..1778
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1890
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1891..2045
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2046..2244
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2245..2413
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2414..2510
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2511..2605
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2606..2856
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2857..2946
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2947..3054
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3055..3168
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3169..3329
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3330..3525
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3526..3785
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3765..3770
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /note="hexamer: AATAAA"
     polyA_site      3785
                     /gene="POLR2B"
                     /gene_synonym="hRPB140; POL2RB; RPB2"
                     /note="major polyA site"
ORIGIN      
        1 acttcgtctt tagctcctgg cgctgctggc ttctgggcgg tttttgtctt ttgatttcaa
       61 gagttaggag ctcgagaacc gtttggcaat atgtacgacg cggatgagga tatgcaatat
      121 gatgaggatg atgatgaaat caccccggat ttgtggcaag aagcatgctg gattgtaatc
      181 agttcctatt ttgacgagaa aggcttggtt agacaacagc tggattcttt tgatgagttt
      241 attcagatgt ctgttcaaag aattgtggaa gacgctcctc ctatagacct acaggctgaa
      301 gctcagcatg ctagtggaga agttgaagaa ccgccacgat atttgctgaa gtttgaacaa
      361 atttatcttt ccaagcctac ccattgggaa agagatggtg ctccttcacc aatgatgccc
      421 aatgaagcta gattaaggaa tctcacgtat tctgctccgc tttatgttga tataacaaaa
      481 acagtcatta aagaaggtga agaacaactt cagactcagc atcagaaaac ttttatagga
      541 aaaattccaa ttatgttgcg gtcaacttac tgccttttga atggcttgac agatcgtgat
      601 ctttgtgagt taaatgaatg ccctttggat cctggtggct atttcattat taatggatca
      661 gaaaaggttc tgattgccca agagaaaatg gcaacaaaca cagtttatgt gtttgccaaa
      721 aaggattcta aatatgccta cacaggagag tgtagatcat gtcttgagaa ttcttcccga
      781 cccaccagta ctatatgggt tagcatgctg gcaagaggag gacagggtgc caagaagagt
      841 gctattggtc agcgcattgt ggcaactcta ccatatatca agcaagaagt tcccatcatt
      901 attgtgttca gagcattagg ttttgtgtcc gacagagata ttttagaaca tattatttat
      961 gattttgaag atccagagat gatggaaatg gttaaacctt ctctcgatga agcttttgtc
     1021 atccaagaac agaatgttgc actaaatttc attggttcaa gaggagcaaa gcctggtgtt
     1081 actaaagaga aaagaattaa atatgcaaag gaagttttac aaaaagaaat gctccctcat
     1141 gttggtgtca gtgatttttg tgagaccaaa aaagcctatt tcttgggata catggttcat
     1201 aggttacttc tggcagcttt gggtagaaga gaactagatg acagagatca ctatggaaac
     1261 aagagattgg atcttgctgg gccgctgctt gcattcttat ttagaggtat gtttaagaat
     1321 ttgcttaaag aagtgcggat ctatgcacag aaatttattg atcgaggaaa ggattttaac
     1381 ttggagttgg caattaaaac acggatcata tctgatggcc taaaatactc tttagctact
     1441 ggaaactggg gtgatcaaaa gaaagctcat caagccagag ctggagtatc tcaggtgtta
     1501 aaccgcctga cttttgcgtc tactctttct cacctgcgtc gtttaaattc tcctattggt
     1561 agagacggca agctagcaaa accaagacag ttgcataata cgttgtgggg aatggtgtgt
     1621 cctgccgaga ccccagaggg ccatgctgta ggacttgtga agaatttagc cttgatggcg
     1681 tatatttcag ttggatctca accatctcca attctggaat ttttagaaga atggagtatg
     1741 gaaaatttag aagaaatttc tcctgcagct attgctgatg caaccaagat ttttgttaat
     1801 ggctgctggg ttggaataca taaagatccc gaacaactta tgaacaccct aaggaaattg
     1861 agacgtcaga tggacatcat tgtgtctgaa gtttctatga tcagagatat tcgagagagg
     1921 gagattcgga tctatacgga tgcaggccgt atttgtagac cacttctgat tgtggaaaaa
     1981 caaaagctac ttttgaagaa gaggcatatt gaccaattga aagagagaga atataacaac
     2041 tatagttggc aggatcttgt ggccagtggg gtagtggagt atattgatac cctggaagaa
     2101 gaaacagtga tgcttgcaat gactccagat gatttacagg agaaagaagt agcttattgt
     2161 tccacatata cacactgtga gattcatccc tcaatgatcc ttggtgtctg tgcatctatt
     2221 attccctttc ctgatcataa ccagtcccct agaaacacat accagtctgc tatgggtaag
     2281 caggctatgg gagtttacat caccaacttc catgttcgca tggacacatt ggcccatgtt
     2341 ctctattatc ctcaaaagcc acttgtgact acacggtcta tggaatatct acgatttaga
     2401 gagctgccag caggcatcaa ctcaattgtg gccattgcat catacactgg atataatcag
     2461 gaagactctg ttatcatgaa tcgttcagct gtagaccgcg gcttcttcag gtctgttttc
     2521 tatcgctcat acaaagaaca ggagtctaaa aaaggatttg atcaagaaga agtttttgag
     2581 aagcctacac gtgaaacatg ccagggcatg aggcatgcca tttacgacaa gctggatgat
     2641 gatggtttga tagctccagg ggttcgtgta tcaggagatg atgttattat aggcaaaaca
     2701 gtcaccttgc ctgaaaatga agatgaattg gagagcacca atagacgcta taccaagaga
     2761 gactgtagca cttttctcag aactagcgag acgggcattg tggatcaggt tatggtaact
     2821 ctcaatcagg aaggatataa attttgtaaa ataagggtac gctctgttag gattccacag
     2881 attggagaca aatttgctag tcgacatggt caaaagggta cttgtggtat tcagtataga
     2941 caagaggata tgcctttcac ctgtgaaggt atcacccctg atatcatcat caatccccat
     3001 gccatcccct ctcgtatgac tattggtcac ttaattgaat gccttcaagg gaaggtatcg
     3061 gctaacaagg gtgaaattgg tgatgccact ccatttaatg atgctgttaa cgtgcagaag
     3121 atttctaatc ttttatctga ttatggctat catctcagag gaaatgaggt cctgtacaat
     3181 gggttcactg gtcgaaaaat cacatcacaa atatttattg gccccactta ttaccagcgt
     3241 ttgaagcata tggtggatga taagattcac tctcgtgcta ggggacctat tcagatcctc
     3301 aatagacagc ccatggaggg tagatctcgt gatggtggcc tgcgttttgg agaaatggaa
     3361 cgagattgtc agattgccca tggagcagcc cagtttttaa gggaaagatt gtttgaggca
     3421 tcagatccat atcaggttca tgtttgcaat ctttgtggaa taatggcgat tgccaacacc
     3481 aggacccata catatgaatg caggggctgc cgcaataaaa cccagatttc tttggtgcga
     3541 atgccttacg catgcaaact attgtttcag gaacttatgt ctatgagtat tgcaccgcga
     3601 atgatgagtg tttagctatt ttacaggagt caacaagata attaaatatc ttggtgtctt
     3661 gtttctattg tgtggctttt taaaaatgac aaatatgtac tgtgttgtga taaaaagtat
     3721 tttatttgtt taatgatatg catgcttttc ttctgtaaat atataataaa tttttgtaga
     3781 tagtc
//
