LOCUS       NM_019014               7527 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens RNA polymerase I subunit B (POLR1B), transcript
            variant 1, mRNA.
ACCESSION   NM_019014 XM_352943
VERSION     NM_019014.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Ford,B.L., Wei,T., Liu,H., Scull,C.E., Najmi,S.M., Pitts,S.,
            Fan,W., Schneider,D.A. and Laiho,M.
  TITLE     Expression of RNA polymerase I catalytic core is influenced by
            RPA12
  JOURNAL   PLoS One 18 (5), e0285660 (2023)
   PUBMED   37167337
  REMARK    GeneRIF: Expression of RNA polymerase I catalytic core is
            influenced by RPA12.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  AUTHORS   Daiss,J.L., Pilsl,M., Straub,K., Bleckmann,A., Hocherl,M.,
            Heiss,F.B., Abascal-Palacios,G., Ramsay,E.P., Tluckova,K.,
            Mars,J.C., Furtges,T., Bruckmann,A., Rudack,T., Bernecky,C.,
            Lamour,V., Panov,K., Vannini,A., Moss,T. and Engel,C.
  TITLE     The human RNA polymerase I structure reveals an HMG-like docking
            domain specific to metazoans
  JOURNAL   Life Sci Alliance 5 (11), e202201568 (2022)
   PUBMED   36271492
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7527)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 7527)
  AUTHORS   Misiaszek,A.D., Girbig,M., Grotsch,H., Baudin,F., Murciano,B.,
            Lafita,A. and Muller,C.W.
  TITLE     Cryo-EM structures of human RNA polymerase I
  JOURNAL   Nat Struct Mol Biol 28 (12), 997-1008 (2021)
   PUBMED   34887565
REFERENCE   5  (bases 1 to 7527)
  AUTHORS   Zhao,D., Liu,W., Chen,K., Wu,Z., Yang,H. and Xu,Y.
  TITLE     Structure of the human RNA polymerase I elongation complex
  JOURNAL   Cell Discov 7 (1), 97 (2021)
   PUBMED   34671025
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7527)
  AUTHORS   Miller,G., Panov,K.I., Friedrich,J.K., Trinkle-Mulcahy,L.,
            Lamond,A.I. and Zomerdijk,J.C.
  TITLE     hRRN3 is essential in the SL1-mediated recruitment of RNA
            Polymerase I to rRNA gene promoters
  JOURNAL   EMBO J 20 (6), 1373-1382 (2001)
   PUBMED   11250903
REFERENCE   7  (bases 1 to 7527)
  AUTHORS   Jansa,P., Mason,S.W., Hoffmann-Rohrer,U. and Grummt,I.
  TITLE     Cloning and functional characterization of PTRF, a novel protein
            which induces dissociation of paused ternary transcription
            complexes
  JOURNAL   EMBO J 17 (10), 2855-2864 (1998)
   PUBMED   9582279
REFERENCE   8  (bases 1 to 7527)
  AUTHORS   Seither,P., Zatsepina,O., Hoffmann,M. and Grummt,I.
  TITLE     Constitutive and strong association of PAF53 with RNA polymerase I
  JOURNAL   Chromosoma 106 (4), 216-225 (1997)
   PUBMED   9254723
REFERENCE   9  (bases 1 to 7527)
  AUTHORS   Seither,P. and Grummt,I.
  TITLE     Molecular cloning of RPA2, the gene encoding the second largest
            subunit of mouse RNA polymerase I
  JOURNAL   Genomics 37 (1), 135-139 (1996)
   PUBMED   8921381
REFERENCE   10 (bases 1 to 7527)
  AUTHORS   Barbosa,M., Jabs,E.W. and Huston,S.
  TITLE     Treacher Collins Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301704
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC012442.7.
            
            On Jul 31, 2019 this sequence version replaced NM_019014.5.
            
            Summary: Eukaryotic RNA polymerase I (pol I) is responsible for the
            transcription of ribosomal RNA (rRNA) genes and production of rRNA,
            the primary component of ribosomes. Pol I is a multisubunit enzyme
            composed of 6 to 14 polypeptides, depending on the species. Most of
            the mass of the pol I complex derives from the 2 largest subunits,
            Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and
            Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.2343229.1,
                                           SRR11853567.17245.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263331.10/ ENSP00000263331.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-207               AC012442.7         95897-96103
            208-375             AC012442.7         100444-100611
            376-522             AC012442.7         100853-100999
            523-655             AC012442.7         102699-102831
            656-792             AC012442.7         104298-104434
            793-1016            AC012442.7         105207-105430
            1017-1188           AC012442.7         106077-106248
            1189-1360           AC012442.7         111342-111513
            1361-1642           AC012442.7         112725-113006
            1643-1776           AC012442.7         117798-117931
            1777-1947           AC012442.7         121399-121569
            1948-2104           AC012442.7         122178-122334
            2105-2301           AC012442.7         125994-126190
            2302-2555           AC012442.7         126994-127247
            2556-7527           AC012442.7         128279-133250
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q14.1"
     gene            1..7527
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /note="RNA polymerase I subunit B"
                     /db_xref="GeneID:84172"
                     /db_xref="HGNC:HGNC:20454"
                     /db_xref="MIM:602000"
     exon            1..207
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..3438
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /EC_number="2.7.7.6"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DNA-directed RNA polymerase I subunit RPA2; DNA-directed
                     RNA polymerase I 135kDa polypeptide; RNA polymerase I
                     subunit 2; DNA-directed RNA polymerase I 135 kDa
                     polypeptide; polymerase (RNA) I polypeptide B, 128kDa;
                     polymerase (RNA) I subunit B"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase I subunit RPA2
                     isoform 1"
                     /protein_id="NP_061887.2"
                     /db_xref="CCDS:CCDS2097.1"
                     /db_xref="GeneID:84172"
                     /db_xref="HGNC:HGNC:20454"
                     /db_xref="MIM:602000"
                     /translation="MDPGSRWRNLPSGPSLKHLTDPSYGIPREQQKAALQELTRAHVE
                     SFNYAVHEGLGLAVQAIPPFEFAFKDERISFTILDAVISPPTVPKGTICKEANVYPAE
                     CRGRRSTYRGKLTADINWAVNGISKGIIKQFLGYVPIMVKSKLCNLRNLPPQALIEHH
                     EEAEEMGGYFIINGIEKVIRMLIMPRRNFPIAMIRPKWKTRGPGYTQYGVSMHCVREE
                     HSAVNMNLHYLENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEDDS
                     FLRNSVSQMLRIVMEEGCSTQKQVLNYLGECFRVKLNVPDWYPNEQAAEFLFNQCICI
                     HLKSNTEKFYMLCLMTRKLFALAKGECMEDNPDSLVNQEVLTPGQLFLMFLKEKLEGW
                     LVSIKIAFDKKAQKTSVSMNTDNLMRIFTMGIDLTKPFEYLFATGNLRSKTGLGLLQD
                     SGLCVVADKLNFIRYLSHFRCVHRGADFAKMRTTTVRRLLPESWGFLCPVHTPDGEPC
                     GLMNHLTAVCEVVTQFVYTASIPALLCNLGVTPIDGAPHRSYSECYPVLLDGVMVGWV
                     DKDLAPGIADSLRHFKVLREKRIPPWMEVVLIPMTGKPSLYPGLFLFTTPCRLVRPVQ
                     NLALGKEELIGTMEQIFMNVAIFEDEVFAGVTTHQELFPHSLLSVIANFIPFSDHNQS
                     PRNMYQCQMGKQTMGFPLLTYQDRSDNKLYRLQTPQSPLVRPSMYDYYDMDNYPIGTN
                     AIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKIKQGDSSLVFGIKP
                     GDPRVLQKLDDDGLPFIGAKLQYGDPYYSYLNLNTGESFVMYYKSKENCVVDNIKVCS
                     NDTGSGKFKCVCITMRVPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMVPDIL
                     FNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYN
                     FYGTERLYSGISGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDRVTNQPIGGRN
                     VQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAHVCVKCGSLLSPLLEKPPPSW
                     SAMRNRKYNCTLCSRSDTIDTVSVPYVFRYFVAELAAMNIKVKLDVV"
     exon            208..375
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /inference="alignment:Splign:2.1.0"
     exon            376..522
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /inference="alignment:Splign:2.1.0"
     exon            523..655
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /inference="alignment:Splign:2.1.0"
     exon            656..792
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /inference="alignment:Splign:2.1.0"
     exon            793..1016
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1188
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1360
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1642
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1776
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1947
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..2104
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2105..2301
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2302..2555
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2556..7527
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3983..3988
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /note="hexamer: AATAAA"
     polyA_site      4005
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /note="major polyA site"
     regulatory      4836..4841
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /note="hexamer: ATTATA"
     polyA_site      4862
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
     regulatory      6534..6539
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /note="hexamer: AATAGA"
     polyA_site      6554
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
     regulatory      7507..7512
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
                     /note="hexamer: TATAAA"
     polyA_site      7527
                     /gene="POLR1B"
                     /gene_synonym="A135; RPA135; RPA2; Rpo1-2; TCS4"
ORIGIN      
        1 gagactggcg tccggtgtgc aggtggccac atggatcctg gcagccggtg gcggaacctg
       61 cccagcgggc ctagcctaaa gcacttgact gacccctctt atggaatccc gcgggaacag
      121 caaaaggcag cgttgcagga gctgacgcgg gcgcacgtgg agtccttcaa ctacgctgtg
      181 cacgagggtc tcggcctcgc ggtgcaggct atacctccct ttgaatttgc tttcaaagat
      241 gagcgtatct cttttactat tctggatgct gttatcagtc cacctacagt tccaaaaggg
      301 accatctgca aagaggccaa tgtttatcca gcagaatgcc ggggccgaag gagtacctac
      361 cgtgggaagt tgacagctga tatcaactgg gcagtgaatg gaatctcaaa aggaatcatt
      421 aagcagtttc ttggctatgt tcccatcatg gtgaaatcca agctttgcaa cttacgtaac
      481 cttcccccac aagccctcat tgagcaccat gaggaggcag aggaaatggg gggctatttt
      541 ataatcaatg gcattgaaaa agtcatccga atgttgatta tgcctcggag aaattttccc
      601 attgcaatga taagaccaaa atggaaaacc agagggcctg gttatactca gtatggagtt
      661 tcaatgcact gtgtgaggga agaacattcc gctgtcaata tgaacctcca ctacttggaa
      721 aatggcactg ttatgttgaa ctttatttac cgaaaagaac tgttctttct tcctttggga
      781 tttgcactta aggcacttgt cagcttttct gattatcaga tctttcagga gctcatcaaa
      841 ggaaaagagg atgattcttt ccttaggaac tctgtttctc agatgttaag gattgtaatg
      901 gaagagggtt gttcgacaca aaaacaggtc cttaactacc taggtgaatg cttcagagta
      961 aaactcaatg ttcctgactg gtacccaaat gagcaagctg cggagttcct gtttaaccag
     1021 tgcatctgta tccacttgaa atccaatact gaaaagtttt atatgctttg tctcatgacg
     1081 cgaaagctct ttgctttagc caaaggagag tgcatggagg acaatcctga tagtttggtg
     1141 aaccaggaag tcctcacacc gggtcagctc ttccttatgt tcctgaagga aaaactggaa
     1201 ggttggttag tgtctattaa aatagctttt gataagaagg ctcagaagac cagtgtttcc
     1261 atgaacactg acaatttgat gaggattttt acaatgggca tagaccttac aaaaccattt
     1321 gaataccttt ttgctactgg gaatctgcgt tctaaaacag gtcttggcct cctacaagat
     1381 tctggacttt gtgttgtggc tgacaagctg aacttcatac gctacctctc ccatttccgc
     1441 tgcgtgcaca gaggggctga ttttgccaag atgaggacca ccacagtacg caggctgctg
     1501 ccagagtcct ggggcttcct ttgtcccgtg cataccccag acggggagcc ctgtggcctg
     1561 atgaaccacc taactgccgt atgtgaggtt gtcacacagt ttgtgtatac ggcatctatt
     1621 ccagctttac tgtgcaactt gggggtcact cccattgatg gagctcccca ccgatcatac
     1681 agtgagtgct accctgtcct gctggacggt gtcatggttg gctgggtgga taaggatctt
     1741 gctccaggca tcgcagattc tcttcgtcat tttaaggtgt tgagagagaa aagaattcct
     1801 ccctggatgg aagtggtcct tatacccatg acaggaaaac caagtctgta cccaggattg
     1861 ttccttttta ccactccttg tagactggta cggcctgtgc agaacttagc attgggcaaa
     1921 gaagagctaa ttggaactat ggaacagatc ttcatgaatg tcgctatctt tgaggatgaa
     1981 gtttttgctg gagttaccac acaccaggaa ctctttccac acagcctgct gagtgtgatt
     2041 gccaacttca tccctttctc tgatcacaac cagagtccac ggaacatgta ccaatgccag
     2101 atgggtaagc aaactatggg ctttccactt ctcacttatc aagaccgatc ggataacaaa
     2161 ctgtatcgtc ttcagactcc tcagagtccc ttggtgagac cctccatgta tgattattat
     2221 gacatggata actatccaat tgggaccaat gccatcgttg ctgtgatttc ttacactggc
     2281 tatgatatgg aagatgccat gattgtgaat aaggcctctt gggaacgagg ctttgcccat
     2341 ggaagtgtct acaagtctga gttcatagac ctctctgaaa aaattaaaca aggagatagt
     2401 agcctggtgt ttggcatcaa acctggtgac ccacgcgttc tgcagaagtt agatgacgat
     2461 ggattgccgt ttataggagc aaaactgcag tacggagatc cgtattacag ctacctcaac
     2521 ctcaacaccg gggaaagttt tgtgatgtac tataagagta aagaaaattg tgttgtggat
     2581 aacatcaaag tgtgcagtaa tgacactggg agtggaaaat tcaagtgtgt ttgcatcact
     2641 atgagagtgc ctcggaaccc aactatcgga gataaatttg ccagtcgcca tgggcagaag
     2701 ggcattttaa gcagattgtg gccggctgag gacatgcctt ttactgagag tgggatggtc
     2761 ccagacattc tgttcaatcc ccatggtttt ccatcccgca tgaccattgg gatgttaatt
     2821 gagagtatgg ccgggaagtc tgcagctttg catggtctct gccatgatgc tacacccttc
     2881 atcttctcag aggagaactc ggccttagaa tactttggtg agatgttaaa ggctgctggc
     2941 tacaatttct atggcaccga gaggttatat agtggcatca gtgggctaga actggaagca
     3001 gacatcttca taggagtggt ttattatcag cgcttacgcc atatggtctc agacaaattt
     3061 caagtaagga caactggagc ccgagacaga gtcaccaacc agcctattgg gggaagaaat
     3121 gtccagggtg gaatccgttt tggggagatg gaacgggatg cgcttttagc tcatggtaca
     3181 tcttttctcc ttcatgaccg cctcttcaac tgctcagatc ggtcggtagc ccatgtgtgt
     3241 gtgaagtgtg gcagtttact ctctccactg ttggagaagc cacccccttc ttggtctgcc
     3301 atgcgcaaca gaaaatacaa ctgtactctg tgtagtcgca gtgacactat cgatactgtt
     3361 tctgtgcctt atgtttttcg gtattttgta gctgaactgg cagctatgaa catcaaagtg
     3421 aaactggatg ttgtttaact tgatgttgac cttttggatt aagaggacta tcagattaaa
     3481 gcaaaatgta attttaattc aatgaagata tcattaccag gttactcttg agatttttca
     3541 acggtgttag aactctcaac caagacctga aaaccaagta tgcaaggttt ctgaatctct
     3601 ctggtagatt aactattgac aatgattttc tgttatcttt gttcaaaaag ttcatgtctt
     3661 ctcaaaatat gaaatattga taaatggaag agcatacggt gacaagtctc ctttccaacc
     3721 ccaggttccc tacaccctgc tctcagcagg cagtgagtgt cacacacctg ttaatccatc
     3781 ttgagcagga cagtactata caaatagaat gcaagctgta atgtaatttt atattttctt
     3841 atagccacgt tgaagtaaaa acaaacaggt acagtgtttt ttaccagctt tatagaagta
     3901 cagttgttac atatttaatg aatacaattt gatgggtctg actatatgca cacacctttg
     3961 ataccatcac cacaatcagg gtaataaaca tacctgtcat ctccacaagt ttcctcctgc
     4021 ccctttgttt tttgcttttt ggttgctgtt gagtttttgt tttgtcttct gtggtaagaa
     4081 cacttaactc aagacctacc ctcttaacaa atctttaagt gcacgatata gtattgttaa
     4141 ttccaggcac catgttgtac aacagatctt tagaccttac ttgtcttgca taactgaagc
     4201 tttatacctg ttgaacaact ctccatttcc ctggccccta gcaaccaccc ttctaccctg
     4261 tttctatgag tttgactatt acagatatct catatagtgg gatcatgcaa tatttgtcct
     4321 gtgactggct tatttcactt agcatagtga aataagattc atccattttg gaagccaggc
     4381 atggtgctgt gcatctatag tccctgctat ttgagaggct gaggtgggag gatcatttga
     4441 gtgcaggagt tcaaggacag cctgggtaat ataggaagac cctgtcttga agaccctgac
     4501 ctcaagtgat ccacccacct cggcctccga aagtgctagg attacaggtg tgagccactg
     4561 tgcctggcct ccggtgagta ttttatattt agtctacact tccatacttg gcttttttct
     4621 gcttttatat tgatctgctt tcatagcagt gtgtagagtg ccacttatgt tttctttctt
     4681 gtgtacagta ttttattgta tggatttacc atcccctgtg tatttaagtt gttccattct
     4741 ttggccatta taactttttt ctgcaaatat tctggtgact tatctttggc cattataaac
     4801 tgttgataat agatcatctt gtatatactt ctgcaattat aagatgtttt ttgatgatga
     4861 aagctttcct aaggtaattc tttcctgagt ttggttcaga tggtctgtca ctaattataa
     4921 ggcagatagg aacagacaga aaaatctatc atttcaagat aggcttagca ggatgggcgc
     4981 agtggctcac gccactaatc ccaacatttt aggaggccta ggcaggagca atcacttgtg
     5041 cctgggagtt ctagactagc ctgggcgaga cttcatctct acaaaaaaag caacaacgac
     5101 aaaaaaaatt agccaagcat agtggcacac ccctgtagtc ccagctactt gggaggctga
     5161 ggtgggagga ttgcttgaac ccaagaggtc gaggctgcag tgagccaaga ttgtgccact
     5221 gcactccagc ctgggtgaca gagcaggacc ctgtctctat tttataaatt aaaaaaggct
     5281 gggtgtggtg gctcacaccc ataatcccaa cactttggct cagcagattg cttgaaccca
     5341 ggaattcaag tccaatctgg gcaacatggg gaaaccccag ctctacaaaa aaaattagcc
     5401 tggtgtggtg gcacatgcct gtagttccag ctactcagga ggctgaggtg ggagaatctc
     5461 ctgagcctgg aaggtccagg cagtgagcca agattgtgcc accacactcc agcctgggcg
     5521 acagaatgag accctgtctc aaaaaaaaaa aaaaaaaaaa agcggggagg cataagaaaa
     5581 aaccaattta atagaaaaga taggctttgc ttcaggaagc tggttgagaa gaagaaggaa
     5641 aaagtcgatt ctactgactg acgtttcccc ctgctgttaa gaatcccaac cacacacttt
     5701 cacacactat tccaggttct ggctactgaa tgatcccaca gctgaggtct attgtcatcg
     5761 ctccacttct atttttagca gcactaaaaa cattcccaaa aaaaatgttt tttagctttt
     5821 taactgtaga ttcaccacta agaaattggc attggaacag tccacagagc ttattcaaat
     5881 ttcacccatt ttacatgcac tcatttgtgt tgcatgtgat atatagttct atttcatttt
     5941 atcacctgtg tagatggatg aaaacagcaa cataagcaag atacagagct gttccgtcat
     6001 cacagagctc tgccatacta tccttttata gccatctcta cctctgtccc ccatttctaa
     6061 cccctggaaa ccactaatct gttcttcata attttcttat ttcaagaatc ttacgtaaat
     6121 aggatcacga agtataacct ttgagaatgg ccttttcact ccattccctt gagatacatc
     6181 caggtagttg catgtatcaa tagttaattc ctttttattg ctacacagta ctccatagta
     6241 tgaatatact atgtacatag catatatatt tatagtttaa ccattcacct tgaacatttt
     6301 ggttgtttcc agtttggggg cattaacaat aaagctgatc tgaacaatca ggtataatag
     6361 tcccccttta tctttgaaga ttatatccca agactcccag tgagtgtctg aaaccatgga
     6421 tagtatatac actatccata tgtgctatat gtactatatg tcctctatat attttttcta
     6481 tacatacata cctttgatgt ttaattaagc actgcaagaa actgacagca ataaatagaa
     6541 caattatact gtaattaaat gaatgtggcc tcaaaatgta ctgttctcac atattttcag
     6601 actgtgggta actgaaactg cataaagcaa aactggctgg gtgcggtggt gcacacctgt
     6661 aatcccagga ctttggcagg ctgaggtgtg cagatcactt gaggtcagga gtttgagacc
     6721 agcctggcca tcatggggaa accccgtctc tattaaaaat acaaaaatta gctgggtgtg
     6781 atggcacacg cctgtaatcc cagctactca ggaggctgag acaggagaat cacttgaacc
     6841 caggaggcag aggttgcagt gagccaagat cacgccactg cacagcaacc tgggcaacag
     6901 agcaagacta gagtctcaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag gaaagcaaaa
     6961 ttgtagataa ggaggactac tatacaggtt attgtgtaaa cataagtttc atttccctgg
     7021 gacaagtgtt caagactggg attgctgggt catatggtag ttgcatattt catttaataa
     7081 actgccaaac ttttcagagt ggctgtgcca ttttacattc ctaccagcaa tgtataagtg
     7141 attcatttct tggcattctg accaaccagc atttggtgat gtcacttttt ttttaaggct
     7201 agtcaagtga agcagtggga gtggaaaaag aacaaataaa tctgtaagtg gttgtgatca
     7261 atttgttgca acaccattgc acttggacca gcccactatt tcttatttta gccattctga
     7321 tagatgtgta gtgatatctc attgtggctt taatttgcat ttctctaatg gctgaaaatg
     7381 ttgaacatct cttcatgtgt ttatttgcca tctgtgtatc ttcagtgaaa tgtctgctgt
     7441 tcatttttta attggattgt ttgtcttctt actgttgaat ttcaagactt cattatatat
     7501 tccagatata aagcctttgt catacaa
//
