LOCUS       NM_015425              12480 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens RNA polymerase I subunit A (POLR1A), mRNA.
ACCESSION   NM_015425 NR_026984
VERSION     NM_015425.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12480)
  AUTHORS   Ford,B.L., Wei,T., Liu,H., Scull,C.E., Najmi,S.M., Pitts,S.,
            Fan,W., Schneider,D.A. and Laiho,M.
  TITLE     Expression of RNA polymerase I catalytic core is influenced by
            RPA12
  JOURNAL   PLoS One 18 (5), e0285660 (2023)
   PUBMED   37167337
  REMARK    GeneRIF: Expression of RNA polymerase I catalytic core is
            influenced by RPA12.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12480)
  AUTHORS   Smallwood,K., Watt,K.E.N., Ide,S., Baltrunaite,K., Brunswick,C.,
            Inskeep,K., Capannari,C., Adam,M.P., Begtrup,A., Bertola,D.R.,
            Demmer,L., Demo,E., Devinsky,O., Gallagher,E.R., Guillen
            Sacoto,M.J., Jech,R., Keren,B., Kussmann,J., Ladda,R.,
            Lansdon,L.A., Lunke,S., Mardy,A., McWalters,K., Person,R.,
            Raiti,L., Saitoh,N., Saunders,C.J., Schnur,R., Skorvanek,M.,
            Sell,S.L., Slavotinek,A., Sullivan,B.R., Stark,Z., Symonds,J.D.,
            Wenger,T., Weber,S., Whalen,S., White,S.M., Winkelmann,J., Zech,M.,
            Zeidler,S., Maeshima,K., Stottmann,R.W., Trainor,P.A. and
            Weaver,K.N.
  TITLE     POLR1A variants underlie phenotypic heterogeneity in craniofacial,
            neural, and cardiac anomalies
  JOURNAL   Am J Hum Genet 110 (5), 809-825 (2023)
   PUBMED   37075751
  REMARK    GeneRIF: POLR1A variants underlie phenotypic heterogeneity in
            craniofacial, neural, and cardiac anomalies.
REFERENCE   3  (bases 1 to 12480)
  AUTHORS   Daiss,J.L., Pilsl,M., Straub,K., Bleckmann,A., Hocherl,M.,
            Heiss,F.B., Abascal-Palacios,G., Ramsay,E.P., Tluckova,K.,
            Mars,J.C., Furtges,T., Bruckmann,A., Rudack,T., Bernecky,C.,
            Lamour,V., Panov,K., Vannini,A., Moss,T. and Engel,C.
  TITLE     The human RNA polymerase I structure reveals an HMG-like docking
            domain specific to metazoans
  JOURNAL   Life Sci Alliance 5 (11), e202201568 (2022)
   PUBMED   36271492
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 12480)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 12480)
  AUTHORS   Misiaszek,A.D., Girbig,M., Grotsch,H., Baudin,F., Murciano,B.,
            Lafita,A. and Muller,C.W.
  TITLE     Cryo-EM structures of human RNA polymerase I
  JOURNAL   Nat Struct Mol Biol 28 (12), 997-1008 (2021)
   PUBMED   34887565
REFERENCE   6  (bases 1 to 12480)
  AUTHORS   Hirschler-Laszkiewicz,I., Cavanaugh,A.H., Mirza,A., Lun,M., Hu,Q.,
            Smink,T. and Rothblum,L.I.
  TITLE     Rrn3 becomes inactivated in the process of ribosomal DNA
            transcription
  JOURNAL   J Biol Chem 278 (21), 18953-18959 (2003)
   PUBMED   12646563
REFERENCE   7  (bases 1 to 12480)
  AUTHORS   Dundr,M., Hoffmann-Rohrer,U., Hu,Q., Grummt,I., Rothblum,L.I.,
            Phair,R.D. and Misteli,T.
  TITLE     A kinetic framework for a mammalian RNA polymerase in vivo
  JOURNAL   Science 298 (5598), 1623-1626 (2002)
   PUBMED   12446911
  REMARK    GeneRIF: analyzed the kinetics of assembly and elongation of the
            RNA polymerase I complex on endogenous ribosomal genes in the
            nuclei of living cells with the use of in vivo microscopy [RPA43]
REFERENCE   8  (bases 1 to 12480)
  AUTHORS   Yuan,X., Zhao,J., Zentgraf,H., Hoffmann-Rohrer,U. and Grummt,I.
  TITLE     Multiple interactions between RNA polymerase I, TIF-IA and TAF(I)
            subunits regulate preinitiation complex assembly at the ribosomal
            gene promoter
  JOURNAL   EMBO Rep 3 (11), 1082-1087 (2002)
   PUBMED   12393749
REFERENCE   9  (bases 1 to 12480)
  AUTHORS   Jansa,P., Mason,S.W., Hoffmann-Rohrer,U. and Grummt,I.
  TITLE     Cloning and functional characterization of PTRF, a novel protein
            which induces dissociation of paused ternary transcription
            complexes
  JOURNAL   EMBO J 17 (10), 2855-2864 (1998)
   PUBMED   9582279
REFERENCE   10 (bases 1 to 12480)
  AUTHORS   Seither,P., Coy,J.F., Pouska,A. and Grummt,I.
  TITLE     Molecular cloning and characterization of the cDNA encoding the
            largest subunit of mouse RNA polymerase I
  JOURNAL   Mol Gen Genet 255 (2), 180-186 (1997)
   PUBMED   9236775
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP363163.1, AK302458.1,
            AK307490.1, AC012511.7 and BC073824.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_015425.5.
            
            Summary: The protein encoded by this gene is the largest subunit of
            the RNA polymerase I complex. The encoded protein represents the
            catalytic subunit of the complex, which transcribes DNA into
            ribosomal RNA precursors. Defects in this gene are a cause of the
            Cincinnati type of acrofacial dysostosis. [provided by RefSeq, May
            2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK302458.1, BC126303.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263857.11/ ENSP00000263857.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 BP363163.1         270-275
            7-2977              AK302458.1         1-2971
            2978-3326           AK307490.1         2967-3315
            3327-4333           AK302458.1         3321-4327
            4334-4334           AC012511.7         134209-134209       c
            4335-5430           AK302458.1         4329-5424
            5431-12148          AC012511.7         122437-129154       c
            12149-12480         BC073824.1         947-1278
FEATURES             Location/Qualifiers
     source          1..12480
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p11.2"
     gene            1..12480
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /note="RNA polymerase I subunit A"
                     /db_xref="GeneID:25885"
                     /db_xref="HGNC:HGNC:17264"
                     /db_xref="MIM:616404"
     exon            1..187
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..5273
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /EC_number="2.7.7.6"
                     /note="DNA-directed RNA polymerase I largest subunit;
                     DNA-directed RNA polymerase I subunit A1; RNA polymerase I
                     194 kDa subunit; polymerase (RNA) I polypeptide A, 194kDa;
                     polymerase (RNA) I subunit A"
                     /codon_start=1
                     /product="DNA-directed RNA polymerase I subunit RPA1"
                     /protein_id="NP_056240.2"
                     /db_xref="CCDS:CCDS42706.1"
                     /db_xref="GeneID:25885"
                     /db_xref="HGNC:HGNC:17264"
                     /db_xref="MIM:616404"
                     /translation="MLISKNMPWRRLQGISFGMYSAEELKKLSVKSITNPRYLDSLGN
                     PSANGLYDLALGPADSKEVCSTCVQDFSNCSGHLGHIELPLTVYNPLLFDKLYLLLRG
                     SCLNCHMLTCPRAVIHLLLCQLRVLEVGALQAVYELERILNRFLEENPDPSASEIREE
                     LEQYTTEIVQNNLLGSQGAHVKNVCESKSKLIALFWKAHMNAKRCPHCKTGRSVVRKE
                     HNSKLTITFPAMVHRTAGQKDSEPLGIEEAQIGKRGYLTPTSAREHLSALWKNEGFFL
                     NYLFSGMDDDGMESRFNPSVFFLDFLVVPPSRYRPVSRLGDQMFTNGQTVNLQAVMKD
                     VVLIRKLLALMAQEQKLPEEVATPTTDEEKDSLIAIDRSFLSTLPGQSLIDKLYNIWI
                     RLQSHVNIVFDSEMDKLMMDKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMY
                     INTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTALS
                     AVDMTQREAVAKQLLTPATGAPKPQGTKIVCRHVKNGDILLLNRQPTLHRPSIQAHRA
                     RILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDG
                     QPLAGLIQDHMVSGASMTTRGCFFTREHYMELVYRGLTDKVGRVKLLSPSILKPFPLW
                     TGKQVVSTLLINIIPEDHIPLNLSGKAKITGKAWVKETPRSVPGFNPDSMCESQVIIR
                     EGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGKVLTCLARLFTAYLQLYRGFTLG
                     VEDILVKPKADVKRQRIIEESTHCGPQAVRAALNLPEAASYDEVRGKWQDAHLGKDQR
                     DFNMIDLKFKEEVNHYSNEINKACMPFGLHRQFPENSLQMMVQSGAKGSTVNTMQISC
                     LLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIKPPEFFFHCMAGR
                     EGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQ
                     FLQPKQFPFLASNYEVIMKSQHLHEVLSRADPKKALHHFRAIKKWQSKHPNTLLRRGA
                     FLSYSQKIQEAVKALKLESENRNGRSPGTQEMLRMWYELDEESRRKYQKKAAACPDPS
                     LSVWRPDIYFASVSETFETKVDDYSQEWAAQTEKSYEKSELSLDRLRTLLQLKWQRSL
                     CEPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTP
                     MMSVPVLNTKKALKRVKSLKKQLTRVCLGEVLQKIDVQESFCMEEKQNKFQVYQLRFQ
                     FLPHAYYQQEKCLRPEDILRFMETRFFKLLMESIKKKNNKASAFRNVNTRRATQRDLD
                     NAGELGRSRGEQEGDEEEEGHIVDAEAEEGDADASDAKRKEKQEEEVDYESEEEEERE
                     GEENDDEDMQEERNPHREGARKTQEQDEEVGLGTEEDPSLPALLTQPRKPTHSQEPQG
                     PEAMERRVQAVREIHPFIDDYQYDTEESLWCQVTVKLPLMKINFDMSSLVVSLAHGAV
                     IYATKGITRCLLNETTNNKNEKELVLNTEGINLPELFKYAEVLDLRRLYSNDIHAIAN
                     TYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIRSNSSP
                     LQQMTFETSFQFLKQATMLGSHDELRSPSACLVVGKVVRGGTGLFELKQPLR"
     exon            188..392
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            393..542
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            543..650
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            651..736
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            737..840
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            841..927
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1033
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1196
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1367
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1490
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1721
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1976
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            1977..2168
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            2169..2318
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            2319..2502
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            2503..2585
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            2586..2744
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            2745..2843
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            2844..2996
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            2997..3079
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            3080..3245
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            3246..3467
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            3468..3682
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            3683..3850
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            3851..3986
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            3987..4144
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            4145..4271
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            4272..4382
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            4383..4688
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            4689..4889
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            4890..5007
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            5008..5172
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     exon            5173..12480
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6338..6343
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /note="hexamer: AAGAAA"
     polyA_site      6368
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /note="major polyA site"
     regulatory      11787..11792
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /note="hexamer: ATTAAA"
     polyA_site      11807
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
     regulatory      12457..12462
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /note="hexamer: AATAAA"
     polyA_site      12480
                     /gene="POLR1A"
                     /gene_synonym="A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;
                     RPO1-4; RPO14"
                     /note="major polyA site"
ORIGIN      
        1 aagcgcacgt tgaatcgcgt ggtgactccg ggcttgaggt tgaattaaga atagtcaggt
       61 ggtgagtgga acgtctcttg gggtgtcgga attcaaaacg gacctggagg atgttgatct
      121 ccaagaacat gccctggcgg cggctgcagg gcatttcctt cgggatgtat tcggctgaag
      181 agctcaagaa attaagtgtt aaatccatta cgaaccctcg atacctggac agcctgggga
      241 acccatcggc aaacggcctg tacgatttag ctttgggccc tgcagattcc aaagaggtgt
      301 gctccacctg cgtgcaggac ttcagcaact gttctgggca cctgggccac attgagctcc
      361 cactcacagt gtataaccct ctcctcttcg ataagctgta cctgctgctt cggggctctt
      421 gtttaaactg ccacatgctg acttgtcccc gggccgtgat tcacctctta ctctgccagc
      481 tgagggttct ggaagtcggg gccctacaag cagtctacga gcttgagaga attctgaaca
      541 ggtttctgga agaaaatccc gatccctctg cctctgaaat tcgggaggaa ttagaacaat
      601 acacaactga aattgtgcag aacaacctcc tggggtccca gggcgcacat gtaaagaacg
      661 tgtgtgagag caagagcaag ctcattgctc tcttctggaa ggcacatatg aatgctaagc
      721 gctgtcccca ctgcaagacc gggcgatccg ttgtccgaaa ggaacacaac agcaagttga
      781 ctatcacgtt tccagccatg gtgcacagga cagctggcca gaaggactct gagcccctgg
      841 gaattgagga agctcagata ggaaaacgag gatacttaac acccaccagt gcccgcgaac
      901 acctttctgc cctgtggaag aatgaaggat tctttctgaa ctaccttttt tcgggaatgg
      961 atgatgatgg tatggaatcc agattcaatc ccagtgtgtt ctttctagat ttcttggtgg
     1021 tgccgccctc aaggtatcgc ccagtcagtc gcctaggaga ccagatgttt actaatggcc
     1081 agacggtgaa cttgcaggct gtcatgaagg atgtagttct gattcgaaaa cttctggcat
     1141 tgatggccca agaacagaag ttgccagagg aagtggccac acccactaca gatgaggaaa
     1201 aagactcttt gattgctatt gaccgatcct ttttgagtac acttccaggc cagtccctca
     1261 tagacaaact ttacaacatt tggattcgcc ttcagagcca cgtcaatatt gtgtttgata
     1321 gcgagatgga caaactaatg atggacaagt acccaggcat taggcagatc ctggagaaga
     1381 aagaaggcct gttccgaaaa cacatgatgg gaaagcgagt ggactacgct gcgcgctcag
     1441 tcatctgccc agacatgtac atcaacacca acgaaattgg aattcccatg gtgtttgcca
     1501 caaaactgac ctacccacag ccagttaccc catggaatgt tcaggaactt aggcaagcgg
     1561 tcatcaacgg ccctaatgtg cacccaggag cctccatggt catcaatgag gacggcagcc
     1621 gcacagccct gagcgctgtg gacatgaccc agcgagaggc cgtggccaag cagcttctga
     1681 ccccagccac gggggcacct aagccccagg ggacaaaaat tgtgtgccgg catgtgaaga
     1741 atggggacat tctgctactg aaccgacagc ccacactgca cagaccctcc atccaggccc
     1801 accgtgcccg catcctgcct gaagagaaag tgctgcggct ccactatgcc aactgcaagg
     1861 cctataatgc cgactttgat ggagacgaga tgaatgccca tttcccccag agtgagctgg
     1921 gccgggccga ggcctacgtc ctggcctgca ctgatcagca gtaccttgtt cccaaggatg
     1981 gccaaccatt ggcgggactg atccaggatc acatggtttc aggggcaagc atgactactc
     2041 ggggttgctt tttcacccgg gagcactata tggagctggt gtaccgagga ctcacggaca
     2101 aagtggggcg cgtgaagctc ctttctcctt ccatcctgaa gccctttccg ctgtggacag
     2161 gaaaacaggt tgtgtcaacg ctgctcataa atataatccc agaggaccac atcccactga
     2221 acttatctgg aaaggcgaaa atcactggga aagcctgggt gaaggaaact cctcgatccg
     2281 ttcctggctt taaccctgac tcgatgtgcg agtcccaggt gatcatcagg gaaggggagc
     2341 tgctctgcgg agtgctggac aaggcgcact atgggagctc cgcctacggc ctggtccact
     2401 gctgctatga gatctatgga ggcgagacca gcggcaaggt tctaacctgc ctggcccgcc
     2461 tcttcaccgc ctacctgcag ctctacagag gcttcacctt gggcgtggaa gacattttgg
     2521 tgaagccaaa ggcagatgtc aagaggcaac gtatcattga agaatccacc cactgcgggc
     2581 cccaggctgt cagggctgca ttaaacctgc cagaagccgc atcatatgat gaggtccgag
     2641 gaaaatggca ggatgcccat ctgggcaagg accagaggga ttttaacatg attgatctga
     2701 agttcaagga ggaagtgaac cattacagca atgagattaa caaggcatgc atgccttttg
     2761 gcctacacag acagttccca gagaacagcc tgcagatgat ggtgcagtcg ggagccaaag
     2821 gttcaactgt gaacacgatg cagatctcgt gcctgctggg ccagattgaa ctggaaggtc
     2881 ggagaccccc gctgatggcg tctggcaagt cactgccctg ctttgagcct tatgagttca
     2941 cccccagggc tggtggcttt gtcactggca ggttcctcac cggcatcaaa cctcctgagt
     3001 tcttcttcca ctgcatggca ggacgagagg gcctggtgga cactgctgtg aaaaccagcc
     3061 gctcaggcta tctccaaagg tgcatcatca agcacctaga ggggctggtc gtgcagtatg
     3121 atctcacggt ccgtgacagt gacggcagtg tggtgcagtt cctgtatggg gaggatggcc
     3181 tggacatccc caagacacag ttcctgcagc ccaagcagtt ccccttcctg gccagcaact
     3241 acgaggtgat aatgaaatca cagcatctcc atgaagtttt atccagagca gatcccaaaa
     3301 aagctctcca ccacttcaga gctatcaaaa aatggcaaag caagcacccc aacaccctgc
     3361 tgagaagagg cgccttcttg agttattccc agaaaattca ggaagctgtg aaagccctga
     3421 aacttgagag tgaaaaccgc aatggccgca gccctgggac tcaggagatg ctgaggatgt
     3481 ggtatgagtt ggatgaggaa agccgaagga aataccagaa gaaggcggcc gcttgtcctg
     3541 accccagtct gtctgtctgg cgtcctgaca tctactttgc atcagtgtca gaaacatttg
     3601 aaacaaaggt tgatgactac agtcaagagt gggcagctca aacagagaag agttatgaga
     3661 aatcagagct ttctctcgac aggttgagga ccttgctgca gctgaagtgg cagcgctcac
     3721 tgtgtgagcc gggcgaggct gtgggcctgc tggctgccca gagcatcgga gagccctcca
     3781 cccagatgac cctcaacacc ttccactttg caggcagagg cgagatgaac gtcaccctgg
     3841 gcattccaag gttgcgggag attctcatgg tggccagcgc caacatcaag acacccatga
     3901 tgagcgtgcc cgtgctcaac accaagaaag ccctgaagag agtgaaaagc ctgaagaagc
     3961 aactcaccag ggtgtgcttg ggggaggtgt tgcagaaaat tgacgtccag gagtccttct
     4021 gtatggaaga aaaacagaac aaattccagg tgtaccagct gcggtttcag ttcctgccac
     4081 atgcatatta ccagcaggag aagtgcctga gacccgagga catcctgcgc ttcatggaaa
     4141 caagattctt taaacttctg atggaatcca tcaaaaagaa gaataataaa gcatcagctt
     4201 tcaggaacgt aaacactcga agagctacac agcgggatct ggacaacgct ggggagttgg
     4261 ggaggagtcg gggagagcag gagggtgatg aggaagagga ggggcacatt gtggatgctg
     4321 aagctgagga gggggacgcc gatgcctctg atgccaaacg caaggagaag caggaggagg
     4381 aggttgatta tgagagtgag gaagaggagg agagggaggg cgaggagaac gacgatgaag
     4441 acatgcagga ggaacgaaat ccccacaggg aaggtgctcg aaagacccaa gagcaagatg
     4501 aagaggtggg cttaggcact gaggaggacc cgtcccttcc cgccctcctg acgcagcccc
     4561 ggaaacccac ccacagccag gagccccagg ggcccgaggc catggagcgc cgggtccagg
     4621 ctgtgcgtga gatccacccg ttcatagatg actaccagta cgacaccgag gagagcctgt
     4681 ggtgccaggt gacagtgaag ctccctctga tgaagatcaa ctttgacatg agctccctgg
     4741 tagtatcttt ggcccatggt gccgtcatct atgcgaccaa gggcatcact cggtgcctcc
     4801 tgaatgaaac aaccaacaat aagaacgaga aggagcttgt gctaaacaca gaaggaatca
     4861 acctcccaga gctattcaag tatgcagagg tcctggatct gcgccgcctc tactccaacg
     4921 acatccacgc catagccaac acgtatggca ttgaggccgc gctgcgggtg atcgagaagg
     4981 agatcaagga tgtgtttgcc gtgtatggca tcgcggtcga ccctcgccat ctctccctgg
     5041 ttgctgatta tatgtgcttc gagggtgttt acaagccact gaatcgcttt gggatccggt
     5101 caaactcttc cccgctacag cagatgacat ttgaaaccag cttccagttt ctgaagcaag
     5161 ccaccatgct gggatcccac gatgagctga ggtctccttc tgcctgcctt gtggtcggga
     5221 aggtcgtcag gggcgggaca ggcctgttcg agctcaagca gcctctgaga tagcagctac
     5281 cccggcacca tctgcccagc tccaaggacc cttggtgagg gcgtggccca gcctgccttc
     5341 tgcatgagag gaccaggaga ctggaatcca gggcagttcc aagtgacagt acagagcaca
     5401 gcagcgacct tgggcctgaa agcagtgggc ctctgagctg ggccagcttc acctggaaag
     5461 tgacagagtt gctcatcctt gcccctccct gtctctggat ttttatcaag gtttaccaag
     5521 tcttctgagt ccccctgaga tggctggggc ctcacctgtg ctgcaggagg cctctgtggc
     5581 ataaccccta aggagaagtc ctgattcacg attcactgag aagaccaagg ggaagccatg
     5641 ctttgctgct ggggacccca ggcacctcca gagtagggaa gcggggttct tttgctgtga
     5701 gtggccaggg acaacagaca agattcctgg gggctcccga tgagcaggaa cgtggagcct
     5761 gctgcccaag gcctgctcct tccggctgct ccagcccctg ggggcagagt ccacaaagag
     5821 tccccatcaa gacttcttcc ctgagtcaag tacagcgtag catagtcctc cacccaccca
     5881 acctctctgc ctggccaggg tcctggccct gccactgtgt ggcgaggtgt ccttctagac
     5941 cacatcagcc ccaaggctgg gagcagtcgc tccagggccg cagcagttca ctcccacaca
     6001 tagaacccag gtcactgctg gggcgattga acaggttgcc tggcttttct ctgctgtcag
     6061 tttggtgtgg aggcctatgt tctgccccat acaccccaca ggccctgctt atgggaagga
     6121 acacaggcct ccagcccaga ggactgtgcc gccctgttct tggccgtcca cgtttcctct
     6181 ccctctagca ccagcaatac atttccctgg catggacaga aaagacagag aggacttata
     6241 caaaggcttt gtaaaaccag aggctagctt ctatctttgt ctactgttat ttcagctcag
     6301 ggcgggtaat taacatcatt ggaactagct attaggaaag aaaaaaaact tggttttttt
     6361 tacaacaatt ttttcccttg gtttgcagtt tctgcatttg gatttgagaa cccagggcgg
     6421 ggattttctt ggcacagtca ggtcttgggt ccctcttcct gtgctttctt gtctgaggcc
     6481 ttagggggaa gagctcagcc tctgcagcct cctccacctc tgcccctggc ctgcctctgc
     6541 tgtgacctcc ttccagagct agctgctggc cctgtggcca ggagaagcag aggggagaag
     6601 cctttatgca gaagctgtgc tagccttggt ctggggtggg gaggctgctg ctgtgacctt
     6661 aggggtaggg ttggggatca agggaggctt tgtcaaggga gagtgaccat gttgctccct
     6721 acatccatgc aataggaccc agcctgcacc tcctaggcct acactgggga aagcctgaca
     6781 gaaatcagta cctcctaacg gggtctgcca ggttcaccaa ggcatgggca cagaggaata
     6841 tgacagtttt gaggcaggca ggaagggccc agcctgcagg gtaagacatt cattctttga
     6901 gttaaaggcc atattcactt gatgatttct acacacacta gaattgtgag agtgtccttg
     6961 aaggagatca caaagtaacc tggtgactgt ggcacagggt ccgtggattc catctctccc
     7021 aggcactgag ggcgcagagg cgaaggagca agccctgcat attggcaggg ctgtgaccat
     7081 cgcacttaga tcagaccccc gacctgtgtt gggatgtgag ggatgccctc ctgccccacc
     7141 catccacatg gttcctgggc agctctgttt tattcctgtg tctttcctga tggatgagta
     7201 ttaagacagc atgtctggtt tttaaacaga catttgggag cttgcacatc ctgatgagca
     7261 gggactcact gtggtggcct tagggaatta cacgctcact ttggtggttc tctatgctcg
     7321 gaagcctctg agagtggcct tggcagctta agatggcaga ggctttggag tcttcttagg
     7381 actgtaagtg cccattgagg atgcatttga gttttggaaa gtaggcccaa gtcaaatctg
     7441 atggatggat tcaagaacat ggactaatct gcgttgtcat ttttaatcaa aatgtaatgg
     7501 agattgatca tctggtgggg ttcacaaact tttcatgaga gctgtgagtt ctttttttga
     7561 gatgcacttt tgcacttgtt acccaggctg agtgcaatgg cgcaatctcg gctccactgc
     7621 aacctccatc tcccgagttc aagcgattct cctgcctcag cctcccgagt agctgagatt
     7681 acaggcatgc gccaccacgc ctggctaatt tttgtatttt tagtagagat ggggtttcac
     7741 catgttggcc aggctagtct cgaactccta atctcaggtg atccgcctgc ctcagcctcc
     7801 caaagtgctg ggtttacagg cgtgagctac tgtgccggcc agagctatga gttctttcag
     7861 aatcctcaga tgccattagc tagccactgt ttggacatcc ttctcacctt gccctttggg
     7921 gtgatatcca tacctacttg cttgcttcta acagtttttt tatttgtttt gtagagacgg
     7981 gttttggttt attgctcagg ctggtttcaa actcctaggc tcaagcggtc ctcccactgt
     8041 ggcctcccaa agtgctgggt ggtgtgagcc accgtgcctg gccagttaat tttttttagt
     8101 gtctcttttt tgggttctca ccagaaacac tatcagcatt cccacagata ttcctgtgag
     8161 tctttgaaac attttctatc ctgcattgag taagaataaa accgaggaag gggtgaagca
     8221 ccttgtctca tccccagtct ggaacttgaa ccttcttttc tcttcacttt gccctacccc
     8281 caaataatat gtacaaatgt ctgtatccat tgagcactaa ctccctattc cttccacctc
     8341 cccagcccct ggcaaccact gttctacgtt ctgtctctac gaatttgact tctctaggtc
     8401 cctcatacaa gtggaaaagt acagcatgtc tttttgtgac cggcttattt cacttagcat
     8461 aatgtcctca aggttcatcc atgctgtagc atgtgtcaga attgccttct tgcttgaggc
     8521 tgctgatacc tcctcatgtg tatataccgt attttgttta atcactcatc tgttggtgga
     8581 cacttgggtt gcctccacct cttggctgtt gcaaataacg ctgtgaacat ggatatacag
     8641 atatctgagg ccaggccagg cgttcacacc tgtaatccca atacgttggg aggccgaggc
     8701 gggcagatca tctgaggtca ggattcgaga ccaccctggc caacatggca aaaccccatc
     8761 tctactaaaa atacaaaaat tagctgggtg tggtggtgcg cgcctataat cccagctact
     8821 cgggaggctg aggcaggaga atcttgaact caggaggtgg aggttgcagt gagccgagat
     8881 tgcgccactg cactccagcc tgggtgacag agtgagattc cgtctcaaaa aaaaaaaaaa
     8941 acaaaaactc agaccctgct ttcctttgaa tatatactag aagtggaatt gctggatcat
     9001 atggtaattc tttaattttt ctgggggaac tgctgtccca ttttccacag cagctacacc
     9061 attttatgtt cccaccaacg gtgtacaaca atttcaattt ctccacatcc ttgttcttgt
     9121 taatagtagc caccctaatg tgtatgaggt gccatctcgt ggtttggtgt gggctgtgtt
     9181 tcaatcagat gttgggaccc ttaagtgttc tgcacctggg gagagttagg aaccacttaa
     9241 ggagtgtctg ttgcttgcca atcttaaaat ttttagtttg cacctgaatc aggggctgtc
     9301 agagtccaga ctgctcttga aatagaaaag actggtcgtt aaaggctggt tcgttgagtc
     9361 tttcatcgtt gagtgatacc acctctgcag atggctcagt ggggcagccc cgtaccacag
     9421 cctgcccttg aggttgtgta gtttagatag gtcacagagt tgctgggcct accgcaggtc
     9481 tcctgaccct ggtgcagttt tacttcaatt acaaccacca aaatagctac agtcaaaaat
     9541 tccaaagttc ctacaccatc aacctccacc ccacttccgt ccaaagtcca cttagaggct
     9601 ctcagttttg gaccttagaa acacttaggg agctttttaa aaagatcgat tctgtatttt
     9661 tagtagagat ggggtttcac ccagctactc aggaggctga ggcaggagaa tcacttgaac
     9721 cggggagttg gaggttgcag tgagccgaga ttatgtcact ccagcctggg caacagagtg
     9781 agactccgtc ttaggaaaaa aaaaaagatt gattctggcc gggcacagtg gctcacgctt
     9841 acagtcccag cactttaaga gactcaggca ggaggatcac ttgagcttag gagtttgaga
     9901 ctagcctggg caacccagtg agaccccgta tctacaaaaa ttagccaggt gttgtgacat
     9961 gtgcctgtgg tcccagctac tcaagaggct gaggtgagag gagcaagtga gcccaggagt
    10021 tcaaggctgc aatgagctgt gatcgcacca ctgcactcca gctcgggtga caagagtgag
    10081 accccgtctg aaaaaaagat tgatcccaag gccttgccca gattctgatt ttattggcct
    10141 ggagtgtggc ctaggcatta gaaaatcttc aagagagtct aaggtgcagc ggaattgaga
    10201 gccacggaca gaaaaagccc taaattgtgg aaatgcacag ccaccgtcac cacctatcgg
    10261 tacgatgaca tctcaaggag tcactggccc taggtttctg cagtaggatc ttaagatctg
    10321 atctctgccc tcaagtgagt gcacgtgcag taaagctgtc atcgtgccat gacagatgtc
    10381 tgcaaggact ttacagtggg gtccaaagga tgccaccatc agctccacct ggggctcaac
    10441 aaagcctgaa agaagaaagg gatgatgctt ccagcagaaa cctggaggcc tgcagctctg
    10501 ccagcccagc acagcaagcg aggccatttg tccagcaggg gcagaaggag ggaactcagc
    10561 cagccagcgt ttcctacaag cagctccatg agaggtttcc acttaaaatg ctaaatcata
    10621 attatttaat cagattcgtt ccttaagttc tttaagcagc atcgctgtcc taccacgagt
    10681 ggtcatcaag gagcagggtt taactcaact ctgagttacc cggcagggta cctgggtaac
    10741 tgcctgggtt gaggtaccca gcaggcttca tgtgcatgtg cacactgccc cctgatagga
    10801 cctggtttat tcacaggctc aggtgtcact gcagctcccc agtgactatc ttcgcgcaag
    10861 ttggactcaa tggcctggga ggtttgtccc agccgacaga tacctgacca tgcccatcct
    10921 caccattccc tcccccatgc tgcatccatc caaccttgat ctccactgtg tcccaggcac
    10981 cctaagatgc acaagctatg tgggccctgc cctcacagaa cttgtagtct tctgccccaa
    11041 acaagccagt tcaccgtctt catgatcagg ccaaaggttg aggtacccag caggcttcat
    11101 atgcacatgc acactgtccc ctataggacc atgccttgcc cagctggctg cactctagag
    11161 ggtgctttgt aggaaggaca gagtggagag gacagtgggg aggtacttgg gtgttcaaca
    11221 gccagaaggg agggaaactg aaaaagaatc aggagtccag ccatcccctc cccacctcct
    11281 gcccccactt ccaccctggc tgctggccct ttgcccttga atatgggtga tataggtgtg
    11341 gctattcagt tccagtcaca gtgctgcctc tatgtctttt gcaagctggg gtcttcctta
    11401 ctcagaaggc ctggaggccc tgccagatgc tgccttccgg actggctgcc cgctgcagtg
    11461 ttttcacggg agccaagcca gccccgggcc tgtagcctcc catgtgacaa agtttcagac
    11521 accacagtcc tcagctggac cacagtaaac acttgccttc cccaacccat gattcttgct
    11581 tggaaacatg aacctgatgt tccacgtgcg ttcagccaat caaacctcaa gaatgtgatt
    11641 gagcagtgag gctgcgtttt gggcaagaca ctgagctctg aaataagact tttcttggaa
    11701 tctcaagccc tcaacttaca gtgggaccct aagaaatgca cttcagtttc cttttatgta
    11761 aaatggtggt aaaattaatt gtgttcatta aattgcacac aactatacat gtttacctat
    11821 acactaagtg gttctcaact tggggcaatt tgccccccag gggacattca gcagtgtctg
    11881 cggacatttt ttgttgctac tggagggagg tgctactggc atctagggag tggaggctag
    11941 agatgctgct aaactacagt ccacaggaca gtctccacag caaagaattg tccagcccca
    12001 aatatcaata atgccgaggt tgaggaaacc ctgccacata cacatagatg cgggttaaca
    12061 ttgtttattt tacaaagtgg aatcatttac attttagcca ttctgataga tgaagcagtg
    12121 gcatcttttt tttttttttt ttttttttga gacggggtct cactctgttg cccaggctgg
    12181 agtgcagggt tgcaatcacg gctcacagca gcctcgaagt cccaggacca agcaaccctc
    12241 ccaccgcagc cttccaagta actgggacta caggcacaca acaccatgcc cagctaattt
    12301 tttaattttt ttgtagagac ggggtttcac catgttgccc aggctcaaac tcctgagctc
    12361 acacgatctg ctgccttcac cttccaaagt gctgggatta caggtgtaag ccactatgcc
    12421 cagcctcatt tgtacttcag tttgcaattc tctattaata aatctaagca tcttttcaca
//
