LOCUS       NM_199420               8757 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens DNA polymerase theta (POLQ), mRNA.
ACCESSION   NM_199420 NM_014125
VERSION     NM_199420.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Carvajal-Maldonado,D., Li,Y., Returan,M., Averill,A.M., Doublie,S.
            and Wood,R.D.
  TITLE     Dynamic stem-loop extension by Pol theta; and templated insertion
            during DNA repair
  JOURNAL   J Biol Chem 300 (7), 107461 (2024)
   PUBMED   38876299
  REMARK    GeneRIF: Dynamic stem-loop extension by Pol theta; and templated
            insertion during DNA repair.
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   Sun,Z., Xu,Y., Shao,B., Dang,P., Hu,S., Sun,H., Chen,C., Wang,C.,
            Liu,J., Liu,Y. and Hu,J.
  TITLE     Exosomal circPOLQ promotes macrophage M2 polarization via
            activating IL-10/STAT3 axis in a colorectal cancer model
  JOURNAL   J Immunother Cancer 12 (5), e008491 (2024)
   PUBMED   38782541
  REMARK    GeneRIF: Exosomal circPOLQ promotes macrophage M2 polarization via
            activating IL-10/STAT3 axis in a colorectal cancer model.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8757)
  AUTHORS   Thomas,C., Avalos-Irving,L., Victorino,J., Green,S., Andrews,M.,
            Rodrigues,N., Ebirim,S., Mudd,A. and Towle-Weicksel,J.B.
  TITLE     Melanoma-Derived DNA Polymerase Theta Variants Exhibit Altered DNA
            Polymerase Activity
  JOURNAL   Biochemistry 63 (9), 1107-1117 (2024)
   PUBMED   38671548
  REMARK    GeneRIF: Melanoma-Derived DNA Polymerase Theta Variants Exhibit
            Altered DNA Polymerase Activity.
REFERENCE   4  (bases 1 to 8757)
  AUTHORS   Carvajal-Maldonado,D., Zahn,K., Jensen,R., Wood,R.D. and Doublie,S.
  TITLE     Human DNA polymerase theta; does not harbor intrinsic nuclease
            activity
  JOURNAL   Mol Cell 84 (8), 1394-1395 (2024)
   PUBMED   38640889
  REMARK    GeneRIF: Human DNA polymerase theta; does not harbor intrinsic
            nuclease activity.
REFERENCE   5  (bases 1 to 8757)
  AUTHORS   Peng,Y., Zheng,W., Chen,Y., Lei,X., Yang,Z., Yang,Y., Liang,W.,
            Sun,K., Li,G. and Yu,J.
  TITLE     POLQ inhibition attenuates the stemness and ferroptosis resistance
            in gastric cancer cells via downregulation of dihydroorotate
            dehydrogenase
  JOURNAL   Cell Death Dis 15 (4), 248 (2024)
   PUBMED   38575587
  REMARK    GeneRIF: POLQ inhibition attenuates the stemness and ferroptosis
            resistance in gastric cancer cells via downregulation of
            dihydroorotate dehydrogenase.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8757)
  AUTHORS   Kawamura,K., Bahar,R., Seimiya,M., Chiyo,M., Wada,A., Okada,S.,
            Hatano,M., Tokuhisa,T., Kimura,H., Watanabe,S., Honda,I.,
            Sakiyama,S., Tagawa,M. and O-Wang,J.
  TITLE     DNA polymerase theta is preferentially expressed in lymphoid
            tissues and upregulated in human cancers
  JOURNAL   Int J Cancer 109 (1), 9-16 (2004)
   PUBMED   14735462
  REMARK    GeneRIF: DNA Pol theta has a specialized function in lymphocytes
            and in tumor progression
REFERENCE   7  (bases 1 to 8757)
  AUTHORS   Seki,M., Marini,F. and Wood,R.D.
  TITLE     POLQ (Pol theta), a DNA polymerase and DNA-dependent ATPase in
            human cells
  JOURNAL   Nucleic Acids Res 31 (21), 6117-6126 (2003)
   PUBMED   14576298
  REMARK    GeneRIF: the isolation of the full-length human DNA POLQ gene, and
            an initial characterization of its gene product, DNA polymerase
            theta
REFERENCE   8  (bases 1 to 8757)
  AUTHORS   Maga,G., Shevelev,I., Ramadan,K., Spadari,S. and Hubscher,U.
  TITLE     DNA polymerase theta purified from human cells is a high-fidelity
            enzyme
  JOURNAL   J Mol Biol 319 (2), 359-369 (2002)
   PUBMED   12051913
  REMARK    GeneRIF: DNA polymerase theta purified from human cells is a
            high-fidelity enzyme.
REFERENCE   9  (bases 1 to 8757)
  AUTHORS   Sharief,F.S., Vojta,P.J., Ropp,P.A. and Copeland,W.C.
  TITLE     Cloning and chromosomal mapping of the human DNA polymerase theta
            (POLQ), the eighth human DNA polymerase
  JOURNAL   Genomics 59 (1), 90-96 (1999)
   PUBMED   10395804
REFERENCE   10 (bases 1 to 8757)
  AUTHORS   Robertson,N.G., Khetarpal,U., Gutierrez-Espeleta,G.A., Bieber,F.R.
            and Morton,C.C.
  TITLE     Isolation of novel and known genes from a human fetal cochlear cDNA
            library using subtractive hybridization and differential screening
  JOURNAL   Genomics 23 (1), 42-50 (1994)
   PUBMED   7829101
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY338826.2, AC069239.16, AB209867.1 and AC079841.10.
            
            On Nov 22, 2018 this sequence version replaced NM_199420.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript matching the
            genomic sequence was available for the full length of the gene. The
            extent of this transcript is supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY338826.2, AB209867.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264233.6/ ENSP00000264233.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-307               AY338826.2         19-325
            308-308             AC069239.16        53292-53292
            309-1061            AY338826.2         327-1079
            1062-1259           AB209867.1         1008-1205
            1260-1547           AY338826.2         1278-1565
            1548-1548           AC069239.16        78263-78263
            1549-3055           AY338826.2         1567-3073
            3056-3056           AC079841.10        147708-147708       c
            3057-7648           AY338826.2         3075-7666
            7649-7649           AC079841.10        93849-93849         c
            7650-8757           AY338826.2         7668-8775
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q13.33"
     gene            1..8757
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /note="DNA polymerase theta"
                     /db_xref="GeneID:10721"
                     /db_xref="HGNC:HGNC:9186"
                     /db_xref="MIM:604419"
     exon            1..274
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..7884
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /EC_number="2.7.7.7"
                     /EC_number="2.7.7.49"
                     /EC_number="3.6.4.12"
                     /note="polymerase (DNA directed), theta; polymerase (DNA)
                     theta; epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="DNA polymerase theta"
                     /protein_id="NP_955452.3"
                     /db_xref="CCDS:CCDS33833.1"
                     /db_xref="GeneID:10721"
                     /db_xref="HGNC:HGNC:9186"
                     /db_xref="MIM:604419"
                     /translation="MNLLRRSGKRRRSESGSDSFSGSGGDSSASPQFLSGSVLSPPPG
                     LGRCLKAAAAGECKPTVPDYERDKLLLANWGLPKAVLEKYHSFGVKKMFEWQAECLLL
                     GQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKALFILPFVSVAKEKKYYLQSL
                     FQEVGIKVDGYMGSTSPSRHFSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDEL
                     HMLGDSHRGYLLELLLTKICYITRKSASCQADLASSLSNAVQIVGMSATLPNLELVAS
                     WLNAELYHTDFRPVPLLESVKVGNSIYDSSMKLVREFEPMLQVKGDEDHVVSLCYETI
                     CDNHSVLLFCPSKKWCEKLADIIAREFYNLHHQAEGLVKPSECPPVILEQKELLEVMD
                     QLRRLPSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGV
                     NLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALL
                     QGSLKPVRSCLQRREGEEVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASMKEG
                     KQGIQRNQESVQLGAIEACVMWLLENEFIQSTEASDGTEGKVYHPTHLGSATLSSSLS
                     PADTLDIFADLQRAMKGFVLENDLHILYLVTPMFEDWTTIDWYRFFCLWEKLPTSMKR
                     VAELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEVPLREINQK
                     YGCNRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDLVR
                     VSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNAVPFKSARKAVDEEEEAVEER
                     RNMRTIWVTGRKGLTEREAAALIVEEARMILQQDLVEMGVQWNPCALLHSSTCSLTHS
                     ESEVKEHTFISQTKSSYKKLTSKNKSNTIFSDSYIKHSPNIVQDLNKSREHTSSFNCN
                     FQNGNQEHQTCSIFRARKRASLDINKEKPGASQNEGKTSDKKVVQTFSQKTKKAPLNF
                     NSEKMSRSFRSWKRRKHLKRSRDSSPLKDSGACRIHLQGQTLSNPSLCEDPFTLDEKK
                     TEFRNSGPFAKNVSLSGKEKDNKTSFPLQIKQNCSWNITLTNDNFVEHIVTGSQSKNV
                     TCQATSVVSEKGRGVAVEAEKINEVLIQNGSKNQNVYMKHHDIHPINQYLRKQSHEQT
                     STITKQKNIIERQMPCEAVSSYINRDSNVTINCERIKLNTEENKPSHFQALGDDISRT
                     VIPSEVLPSAGAFSKSEGQHENFLNISRLQEKTGTYTTNKTKNNHVSDLGLVLCDFED
                     SFYLDTQSEKIIQQMATENAKLGAKDTNLAAGIMQKSLVQQNSMNSFQKECHIPFPAE
                     QHPLGATKIDHLDLKTVGTMKQSSDSHGVDILTPESPIFHSPILLEENGLFLKKNEVS
                     VTDSQLNSFLQGYQTQETVKPVILLIPQKRTPTGVEGECLPVPETSLNMSDSLLFDSF
                     SDDYLVKEQLPDMQMKEPLPSEVTSNHFSDSLCLQEDLIKKSNVNENQDTHQQLTCSN
                     DESIIFSEMDSVQMVEALDNVDIFPVQEKNHTVVSPRALELSDPVLDEHHQGDQDGGD
                     QDERAEKSKLTGTRQNHSFIWSGASFDLSPGLQRILDKVSSPLENEKLKSMTINFSSL
                     NRKNTELNEEQEVISNLETKQVQGISFSSNNEVKSKIEMLENNANHDETSSLLPRKES
                     NIVDDNGLIPPTPIPTSASKLTFPGILETPVNPWKTNNVLQPGESYLFGSPSDIKNHD
                     LSPGSRNGFKDNSPISDTSFSLQLSQDGLQLTPASSSSESLSIIDVASDQNLFQTFIK
                     EWRCKKRFSISLACEKIRSLTSSKTATIGSRFKQASSPQEIPIRDDGFPIKGCDDTLV
                     VGLAVCWGGRDAYYFSLQKEQKHSEISASLVPPSLDPSLTLKDRMWYLQSCLRKESDK
                     ECSVVIYDFIQSYKILLLSCGISLEQSYEDPKVACWLLDPDSQEPTLHSIVTSFLPHE
                     LPLLEGMETSQGIQSLGLNAGSEHSGRYRASVESILIFNSMNQLNSLLQKENLQDVFR
                     KVEMPSQYCLALLELNGIGFSTAECESQKHIMQAKLDAIETQAYQLAGHSFSFTSSDD
                     IAEVLFLELKLPPNREMKNQGSKKTLGSTRRGIDNGRKLRLGRQFSTSKDVLNKLKAL
                     HPLPGLILEWRRITNAITKVVFPLQREKCLNPFLGMERIYPVSQSHTATGRITFTEPN
                     IQNVPRDFEIKMPTLVGESPPSQAVGKGLLPMGRGKYKKGFSVNPRCQAQMEERAADR
                     GMPFSISMRHAFVPFPGGSILAADYSQLELRILAHLSHDRRLIQVLNTGADVFRSIAA
                     EWKMIEPESVGDDLRQQAKQICYGIIYGMGAKSLGEQMGIKENDAACYIDSFKSRYTG
                     INQFMTETVKNCKRDGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADI
                     VKIATVNIQKQLETFHSTFKSHGHREGMLQSDQTGLSRKRKLQGMFCPIRGGFFILQL
                     HDELLYEVAEEDVVQVAQIVKNEMESAVKLSVKLKVKVKIGASWGELKDFDV"
     exon            275..454
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            455..585
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            586..742
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            743..851
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            852..1071
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1219
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1366
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1579
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1722
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1927
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..2070
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            2071..2264
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            2265..2389
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            2390..2633
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            2634..5740
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            5741..5884
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            5885..6081
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            6082..6322
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            6323..6516
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            6517..6654
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            6655..6829
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            6830..6956
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            6957..7078
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            7079..7263
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            7264..7375
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            7376..7500
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            7501..7654
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            7655..7770
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     exon            7771..8757
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8526..8531
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /note="hexamer: AATAAA"
     polyA_site      8564
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /note="major polyA site"
     regulatory      8741..8746
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /note="hexamer: AATGAA"
     polyA_site      8757
                     /gene="POLQ"
                     /gene_synonym="PRO0327"
                     /note="major polyA site"
ORIGIN      
        1 gagtttgaag actggcggga agatgtccgc agctgttgcc aggccagggt tctcccgaga
       61 gggaggacgc tgggactgtg gcttgccctg atcggccgag aagagtttgc catgaatctt
      121 ctgcgtcgga gtgggaaacg gcggcgttca gaatcaggct cagattcgtt ctcgggaagc
      181 ggcggtgaca gcagtgccag cccccagttc ctctccgggt ccgtgctgag cccgccgccc
      241 ggccttggtc gctgcctgaa ggccgcagct gcaggagaat gcaagcctac agttcctgac
      301 tacgaaagag acaagctact attggcaaac tggggacttc ctaaagcagt tctggaaaaa
      361 taccacagtt ttggtgtaaa aaagatgttt gaatggcagg cagagtgcct tttgcttgga
      421 caagtcctgg aaggaaagaa tttagtttat tcagctccta caagtgctgg gaagactctt
      481 gtggcagaat tacttatttt gaagcgggtt ttggaaatgc ggaagaaagc tttgtttatt
      541 cttccctttg tttctgtggc taaagagaag aaatactacc tccagagtct gtttcaggaa
      601 gtaggaataa aagtagacgg ttatatgggc agcacctctc catcaaggca tttctcttca
      661 ttggatattg cagtctgcac aattgagaga gccaatggtc tgatcaatcg cctcatagag
      721 gaaaataaga tggatctgtt aggaatggtg gttgtggatg aattacatat gctgggagac
      781 tctcaccgag ggtatctgct ggaacttttg ctgaccaaga tttgctatat tactcggaaa
      841 tcagcatctt gtcaggcaga tctagccagt tctctgtcta atgctgtgca aatcgttggc
      901 atgagtgcta cccttcctaa tttggagctt gtggcttcct ggttgaatgc tgaactctac
      961 cataccgact ttcgccctgt accgcttttg gagtcagtaa aagttggaaa ttccatatat
     1021 gactcttcaa tgaaacttgt gagggaattt gagcccatgc tacaagtgaa gggagatgag
     1081 gaccatgttg ttagtttatg ttatgagacg atttgtgata accattcagt attacttttt
     1141 tgtccatcaa agaaatggtg tgagaagctg gcagatatca ttgctcgaga gttttataat
     1201 ctacatcatc aagctgaggg attggtgaaa ccctctgaat gcccaccagt aattctggaa
     1261 caaaaagaac tcctggaagt gatggatcag ttaagacgtt tgccttcagg actggactct
     1321 gtattacaga aaactgtacc atggggagta gcatttcatc atgcaggtct tacttttgag
     1381 gagagggata tcattgaagg agcctttcgt caaggtctca ttcgggtctt ggcggcaact
     1441 tctactcttt cttctggggt gaatttacct gcacgtcgtg tgattattcg aacccctatt
     1501 tttggtggtc gacctctaga tattcttact tataagcaga tggttggccg tgctggcagg
     1561 aaaggagtgg acacagtagg cgagagtatc ttaatttgta agaactctga gaaatcaaaa
     1621 ggcatagctc tccttcaggg ttctctaaag cctgttcgca gctgtctgca aagacgagaa
     1681 ggagaagaag taactggcag catgatacga gctattctgg agataatagt tggtggagtg
     1741 gcaagtacat cacaagatat gcatacttat gctgcctgca catttttggc tgcaagtatg
     1801 aaagaaggga agcaaggaat tcagagaaat caagagtctg ttcagcttgg agcgattgag
     1861 gcctgtgtga tgtggctact agaaaatgaa ttcatccaga gtacagaagc cagtgatgga
     1921 acagaaggaa aggtgtatca tccaacacat cttggttcgg ccactctttc ttcttcactt
     1981 tctccagctg atactttaga tatttttgct gacctgcaaa gagcaatgaa gggctttgtt
     2041 ttagagaatg atcttcatat tctctatctg gttacaccta tgtttgagga ttggactact
     2101 attgattggt atcgattttt ctgtttatgg gagaagttgc caacttcaat gaaaagggtg
     2161 gcagagctag tgggagttga agaggggttc ttggcccgtt gtgtgaaagg aaaagtagta
     2221 gccagaactg agagacagca tcgacaaatg gccatccata aaaggttttt caccagtctt
     2281 gtgctattag atttaatcag tgaagttccc ttaagggaaa taaatcagaa atatggatgc
     2341 aatcgtgggc agattcaatc tttgcaacag tcagctgctg tttatgcagg gatgattaca
     2401 gtattttcca accgtctggg ctggcacaac atggaactac tactttccca atttcagaag
     2461 cgtcttacgt ttggcatcca gagggagctg tgtgacctgg ttcgggtatc cttactaaat
     2521 gctcagagag ccagggttct ctatgcttct ggctttcata ctgtggcaga ccttgctaga
     2581 gcaaatattg tggaggtgga ggtgattctg aaaaatgctg tgcctttcaa aagtgcccgg
     2641 aaggcagtgg atgaggaaga ggaagcagtt gaagaacgtc gcaatatgcg aactatctgg
     2701 gtgactggca gaaaaggttt aactgaaagg gaagcagcag cccttatagt ggaagaagcc
     2761 agaatgattc tgcagcagga cttagttgaa atgggagtgc aatggaatcc atgtgccctg
     2821 ttacattcta gtacatgctc attgactcat agtgagtccg aagtaaagga acacacattt
     2881 atatcccaaa ctaagagttc ttataaaaaa ttaacatcaa agaacaaaag taacacaata
     2941 tttagtgatt cttatattaa gcattcacca aatatagtgc aagacttaaa taaaagtaga
     3001 gagcatacaa gttcctttaa ttgtaatttc cagaatggga atcaagaaca tcagacatgt
     3061 tccattttca gagcaagaaa acgggcctct ttagatataa ataaagagaa gccaggagcc
     3121 tctcagaatg aggggaaaac aagtgataag aaagttgttc agactttttc acagaaaaca
     3181 aaaaaggcac ctttgaattt caattcagaa aagatgagca gaagttttcg atcttggaaa
     3241 cgtagaaagc atctaaagcg atctagggac agcagccccc tgaaagactc tggagcgtgt
     3301 agaatccatt tacaaggaca gactctgtct aatcctagtc tttgtgaaga cccgtttacc
     3361 ttagatgaga agaaaacgga atttagaaat tcagggccat ttgctaaaaa tgtatctttg
     3421 agtggtaagg aaaaagataa taaaacatca ttcccattac aaataaagca aaattgttca
     3481 tggaacataa cactaactaa tgataatttt gtggagcata ttgtcacagg atctcagagt
     3541 aaaaatgtga cttgtcaggc cactagtgtg gttagtgaaa agggcagagg agtagctgtt
     3601 gaggcagaaa aaataaatga agtgctgata caaaatggtt caaaaaacca gaatgtttat
     3661 atgaaacacc atgacatcca tccaattaac cagtacctgc gaaagcaatc tcatgaacag
     3721 acaagcacta ttaccaaaca gaaaaatata atagagagac aaatgccctg tgaagcagtc
     3781 agtagttaca taaatagaga ctcaaatgtt actatcaatt gtgaaaggat aaagcttaat
     3841 acagaggaaa ataaaccaag tcattttcag gcattaggag atgatataag cagaactgtg
     3901 atacccagtg aagtacttcc atcagctgga gcatttagca aatcagaagg ccagcatgag
     3961 aattttctaa atatttctag actacaagaa aaaacaggta cttatacaac aaacaaaact
     4021 aaaaataatc atgtttctga cttaggttta gtcctctgtg attttgaaga tagtttctat
     4081 ctggatactc agtcagagaa aataatacaa cagatggcaa ctgaaaatgc caaactagga
     4141 gcaaaggaca ccaacctggc agcagggata atgcagaaga gcttagtcca acagaactca
     4201 atgaactctt ttcagaagga gtgtcacatt ccttttcctg ctgaacagca ccctctagga
     4261 gcgactaaga tagatcattt ggaccttaag actgtaggta ctatgaaaca aagcagtgat
     4321 tcacatgggg ttgatatcct gactccagaa agcccgattt tccattctcc aatactattg
     4381 gaggaaaatg gtcttttttt aaaaaagaat gaagtttctg ttactgattc acaattaaat
     4441 agttttcttc aaggttatca aacacaagaa actgtgaaac cagttatact tctgattcct
     4501 caaaagagaa ctcccactgg tgtagaagga gaatgtcttc cagttcctga aacaagtttg
     4561 aatatgagtg atagtttact atttgatagc ttcagtgatg actatctagt aaaagaacaa
     4621 ttacctgata tgcaaatgaa agaacccctt ccttcagaag taacatcaaa ccattttagt
     4681 gattctctgt gtctacaaga agacctaatt aaaaaatcaa atgtaaatga gaatcaagat
     4741 acccaccagc agttgacttg ttccaatgat gaatctatta tattttcaga aatggattct
     4801 gttcagatgg ttgaagcttt ggacaatgtg gatatatttc ctgtccaaga gaagaatcat
     4861 actgtagtat ctcctagagc attagaacta agtgatccag tacttgatga gcaccaccaa
     4921 ggtgatcaag atggaggaga tcaagatgaa agggctgaaa aatcaaaatt aactgggacc
     4981 aggcaaaatc attcattcat atggtcaggg gcatcatttg atctaagtcc aggactgcaa
     5041 aggattttag ataaagtatc cagtcctcta gaaaatgaaa agctaaaatc aatgactata
     5101 aacttttcca gtttgaatag aaaaaataca gagttaaatg aagaacaaga agttatttca
     5161 aacttggaga caaaacaagt gcagggaatt tcattttctt ctaataatga agtaaaaagc
     5221 aagattgaga tgctagaaaa caatgccaat catgatgaaa cctcatccct cttacctcgt
     5281 aaagaaagta atatagttga tgataatggt ctcattcctc ctacacccat tccaacatct
     5341 gcttctaagc tgacatttcc agggattctt gaaacacctg taaacccttg gaaaactaat
     5401 aatgttttac aacctggtga aagttattta tttggctcac cttcagatat taaaaaccac
     5461 gatttaagtc cagggagtag aaatgggttc aaagacaaca gccctattag tgacacaagc
     5521 ttttcacttc agttatcaca ggatggatta cagttaactc cagcctcaag cagttcagaa
     5581 agtttgtcca taattgatgt agcaagtgac caaaatcttt tccaaacatt cattaaggag
     5641 tggcggtgca aaaagcgatt ttccatctca ctggcttgtg aaaagattag aagtttgaca
     5701 tcttctaaaa ctgctactat tggcagtagg tttaagcaag ctagctcacc tcaggaaatt
     5761 cctattagag atgatggatt tcccattaaa ggttgtgatg acaccttggt ggttggactg
     5821 gcagtatgct ggggtggaag ggatgcctat tatttttcac tgcagaagga acaaaagcat
     5881 tctgaaatta gtgccagttt ggttccacct tctttagatc caagcctgac tttgaaagac
     5941 aggatgtggt accttcaatc ttgcttgcga aaggaatctg ataaagaatg ttctgttgtc
     6001 atctatgact tcatccagag ctataaaatt cttcttcttt cttgtggcat ctccttggag
     6061 caaagttatg aagatcctaa ggtggcatgc tggttactag atccagattc tcaggagccg
     6121 actcttcata gcatagttac cagttttctt cctcatgagc ttccactcct agaagggatg
     6181 gagaccagcc aagggattca aagcctgggg ctaaatgctg gcagtgagca ttctgggcga
     6241 tacagagcat ctgtggagtc cattctcatc ttcaactcta tgaatcagct caactctttg
     6301 ttgcagaagg aaaaccttca agatgttttc cgtaaggtgg aaatgccctc tcagtactgc
     6361 ttggccttgc tagaactaaa tggaattggc tttagtactg cagaatgtga aagtcagaaa
     6421 catataatgc aagccaagct ggatgcaatt gagacccagg cctatcaact agctggccac
     6481 agtttttctt tcaccagttc agatgacatc gctgaggttt tatttttgga attgaagttg
     6541 cccccaaata gagagatgaa aaaccaaggc agcaagaaaa ctctgggttc taccagaaga
     6601 gggattgaca atggacgcaa gctaaggctg ggaagacagt tcagcactag taaggacgtt
     6661 ttaaataaat taaaggcatt acatccttta ccaggcttga tattagaatg gagaagaatc
     6721 actaatgcta ttaccaaagt ggtctttccc cttcagcggg aaaagtgtct taatcctttt
     6781 cttggaatgg aaagaatcta tcctgtatca cagtcgcaca ctgctacagg acgaataacc
     6841 tttacagaac caaatattca gaatgtgcca agagattttg aaatcaaaat gccaacacta
     6901 gtaggagaaa gcccaccttc tcaagctgta ggcaaaggcc tacttcccat gggcagagga
     6961 aaatataaga agggtttcag cgtgaatcct agatgccagg cacagatgga ggagagagct
     7021 gcagacagag gaatgccatt ttcaattagc atgcgacatg cctttgtgcc tttcccaggt
     7081 ggttcaatac tggctgctga ctactctcag cttgaactga ggatcttggc tcatttatcc
     7141 catgatcgtc gtctcattca agtgttaaac actggagctg atgttttcag gagcattgca
     7201 gcagagtgga agatgattga gccagagtct gttggggatg atctgaggca gcaggcaaaa
     7261 cagatttgct atgggatcat ttatggaatg ggagctaaat ctttgggaga gcagatgggc
     7321 attaaagaaa atgatgctgc atgctatatt gactccttca aatccagata cacagggatt
     7381 aatcaattca tgacagagac agtgaagaat tgtaaaagag acggatttgt tcagaccatt
     7441 ttgggaaggc gtagatattt gccaggaatc aaagacaaca acccttatcg taaagctcac
     7501 gctgagcgtc aagctatcaa cacaatagtc caaggatcag cagctgatat tgtcaaaata
     7561 gccacagtta acattcagaa gcaattagag accttccact caaccttcaa atcccatggt
     7621 catcgagagg gtatgctcca aagtgaccaa acaggattgt cacgaaagag aaaactgcaa
     7681 gggatgttct gcccaatcag aggaggcttc ttcatccttc aactccatga tgaactccta
     7741 tatgaagtgg cagaagaaga tgttgttcag gtagctcaga ttgtcaagaa tgaaatggaa
     7801 agtgctgtaa aactgtctgt gaaattgaaa gtgaaagtga aaataggcgc cagctgggga
     7861 gagctaaagg actttgatgt gtaactgtgc tgttgatgaa gtcctcccag ggaagcctgt
     7921 gcagatgcag tcacctggaa agaacagaga ttaccctttc acctacctca gcaaaacaaa
     7981 ctttcaagtc ttgatagact tagcctagta attttatagt gagagtttca aactatatat
     8041 cagtgtctat agcatcaaaa acttctgggg gcgtggggga agtagaatac caagtataat
     8101 agttacattc actttcaaag agcatctatg aatttgcctt ttgtaactta ctgtggcttt
     8161 aaacatattc agaacagatg cttgaaatat gcacttagca ctttggttcc acatctgtct
     8221 gggtaaacca tgaagaaaat gaagctgctg cctcaatcga cccagacagc agccataggc
     8281 agataaagat ttggtttcac cctggtggtg gtaggcatcg tgtgtgactt tttttcctct
     8341 aatatcaatt ttacagtacg gaaatagtat tttaaaatag tattggctaa taaattatga
     8401 attctataaa gtagtaagac ttggtatggt tggagtgtag gaatgaatat tcatgaaatg
     8461 tttcttattg cttttccttc cctaattcat acaatgaatg tatttggaat acttacatat
     8521 tataaaataa actatacctc ttcaagaggt atcctgttct gtaagatcag atgtttttat
     8581 tgcaggtcaa tataatactg ccagagacag aaaatacccc cttatcagtc ccttagtgcc
     8641 tctttctgtt tgtggcatgg tgagaaaacc catgctgaaa agattgtact ttgtgatccc
     8701 aatcagaggg atggagctaa tctttttgct gttgaaataa aatgaattta tgagaaa
//
