LOCUS       NM_001174085            2442 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens DNA polymerase lambda (POLL), transcript variant 3,
            mRNA.
ACCESSION   NM_001174085
VERSION     NM_001174085.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ghazi,M., Khanna,S., Subramaniam,Y., Rengaraju,J., Sultan,F.,
            Gupta,I., Sharma,K., Chandna,S., Gokhale,R.S. and Natarajan,V.T.
  TITLE     Sustained pigmentation causes DNA damage and invokes translesion
            polymerase Polkappa for repair in melanocytes
  JOURNAL   Nucleic Acids Res 51 (19), 10451-10466 (2023)
   PUBMED   37697436
  REMARK    GeneRIF: Sustained pigmentation causes DNA damage and invokes
            translesion polymerase Polkappa for repair in melanocytes.
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Mentegari,E., Bertoletti,F., Kissova,M., Zucca,E., Galli,S.,
            Tagliavini,G., Garbelli,A., Maffia,A., Bione,S., Ferrari,E.,
            Fagagna,F.D.D., Francia,S., Sabbioneda,S., Chen,L.Y., Lingner,J.,
            Bergoglio,V., Hoffmann,J.S., Hubscher,U., Crespan,E. and Maga,G.
  TITLE     A Role for Human DNA Polymerase lambda in Alternative Lengthening
            of Telomeres
  JOURNAL   Int J Mol Sci 22 (5), 2365 (2021)
   PUBMED   33673424
  REMARK    GeneRIF: A Role for Human DNA Polymerase lambda in Alternative
            Lengthening of Telomeres.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2442)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2442)
  AUTHORS   Craxton,A., Munnur,D., Jukes-Jones,R., Skalka,G., Langlais,C.,
            Cain,K. and Malewicz,M.
  TITLE     PAXX and its paralogs synergistically direct DNA polymerase lambda
            activity in DNA repair
  JOURNAL   Nat Commun 9 (1), 3877 (2018)
   PUBMED   30250067
  REMARK    GeneRIF: PAXX, XLF and XRCC4 synergise in the efficient DNA
            double-strand breaks recruitment, substrate recognition and
            stimulation of Pol lambda enzymatic activity during nonhomologous
            end joining DNA repair.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2442)
  AUTHORS   van Loon,B., Hubscher,U. and Maga,G.
  TITLE     Living on the Edge: DNA Polymerase Lambda between Genome Stability
            and Mutagenesis
  JOURNAL   Chem Res Toxicol 30 (11), 1936-1941 (2017)
   PUBMED   28841305
  REMARK    GeneRIF: When mutated or deregulated, DNA polymerase lambda can
            also be a source of genetic instability. Its multiple roles in DNA
            damage tolerance and its ability in promoting tumor progression
            make it also a possible target for novel anticancer approaches.
            [review]
            Review article
REFERENCE   6  (bases 1 to 2442)
  AUTHORS   Haracska,L., Unk,I., Johnson,R.E., Phillips,B.B., Hurwitz,J.,
            Prakash,L. and Prakash,S.
  TITLE     Stimulation of DNA synthesis activity of human DNA polymerase kappa
            by PCNA
  JOURNAL   Mol Cell Biol 22 (3), 784-791 (2002)
   PUBMED   11784855
REFERENCE   7  (bases 1 to 2442)
  AUTHORS   Garcia-Diaz,M., Bebenek,K., Kunkel,T.A. and Blanco,L.
  TITLE     Identification of an intrinsic 5'-deoxyribose-5-phosphate lyase
            activity in human DNA polymerase lambda: a possible role in base
            excision repair
  JOURNAL   J Biol Chem 276 (37), 34659-34663 (2001)
   PUBMED   11457865
REFERENCE   8  (bases 1 to 2442)
  AUTHORS   Nagasawa,K., Kitamura,K., Yasui,A., Nimura,Y., Ikeda,K., Hirai,M.,
            Matsukage,A. and Nakanishi,M.
  TITLE     Identification and characterization of human DNA polymerase beta 2,
            a DNA polymerase beta -related enzyme
  JOURNAL   J Biol Chem 275 (40), 31233-31238 (2000)
   PUBMED   10887191
REFERENCE   9  (bases 1 to 2442)
  AUTHORS   Aoufouchi,S., Flatter,E., Dahan,A., Faili,A., Bertocci,B.,
            Storck,S., Delbos,F., Cocea,L., Gupta,N., Weill,J.C. and
            Reynaud,C.A.
  TITLE     Two novel human and mouse DNA polymerases of the polX family
  JOURNAL   Nucleic Acids Res 28 (18), 3684-3693 (2000)
   PUBMED   10982892
REFERENCE   10 (bases 1 to 2442)
  AUTHORS   Garcia-Diaz,M., Dominguez,O., Lopez-Fernandez,L.A., de Lera,L.T.,
            Saniger,M.L., Ruiz,J.F., Parraga,M., Garcia-Ortiz,M.J.,
            Kirchhoff,T., del Mazo,J., Bernad,A. and Blanco,L.
  TITLE     DNA polymerase lambda (Pol lambda), a novel eukaryotic DNA
            polymerase with a potential role in meiosis
  JOURNAL   J Mol Biol 301 (4), 851-867 (2000)
   PUBMED   10966791
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC409528.1, AK292225.1 and
            BC068529.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001174085.1.
            
            Summary: This gene encodes a DNA polymerase. DNA polymerases
            catalyze DNA-template-directed extension of the 3'-end of a DNA
            strand. This particular polymerase, which is a member of the X
            family of DNA polymerases, likely plays a role in non-homologous
            end joining and other DNA repair processes. Alternatively spliced
            transcript variants have been described. [provided by RefSeq, Mar
            2010].
            
            Transcript Variant: This variant (3) differs in the 5' UTR and uses
            an alternate in-frame splice site in the middle portion of the
            coding region, compared to variant 1. This results in a shorter
            protein (isoform b, also known as lambda2), compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK292225.1, SRR14038195.633330.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                DC409528.1         1-65
            66-2226             AK292225.1         1-2161
            2227-2442           BC068529.1         2468-2683
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q24.32"
     gene            1..2442
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /note="DNA polymerase lambda"
                     /db_xref="GeneID:27343"
                     /db_xref="HGNC:HGNC:9184"
                     /db_xref="MIM:606343"
     exon            1..373
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     exon            374..534
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     CDS             420..1871
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /EC_number="2.7.7.7"
                     /note="isoform b is encoded by transcript variant 3; DNA
                     polymerase beta-2; polymerase (DNA) lambda; DNA polymerase
                     beta-N; polymerase (DNA directed), lambda; DNA polymerase
                     kappa"
                     /codon_start=1
                     /product="DNA polymerase lambda isoform b"
                     /protein_id="NP_001167556.1"
                     /db_xref="GeneID:27343"
                     /db_xref="HGNC:HGNC:9184"
                     /db_xref="MIM:606343"
                     /translation="MDPRGILKAFPKRQKIHADASSKVLAKIPRREEGEEAEEWLSSL
                     RAHVVRTGIGRARAELFEKQIVQHGGQLCPAQGPGVTHIVVDEGMDYERALRLLRLPQ
                     LPPGAQLVKSAWLSLCLQERRLVDVAGFSIFIPSRYLDHPQPSKAEQDASIPPGTHEA
                     LLQTALSPPPPPTRPVSPPQKAKEAPNTQAQPISDDEASDGEETQEACSIPGIGKRMA
                     EKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQMWYQQGFRSLEDIRSQAS
                     LTTQQAIGLKHYSDFLERMPREEATEIEQTVQKAAQAFNSGLLCVACGSYRRGKATCG
                     DVDVLITHPDGRSHRGIFSRLLDSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGR
                     RHRRLDIIVVPYSEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHG
                     CKVGPGRVLPTPTEKDVFRLLGLPYREPAERDW"
     exon            535..829
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     exon            830..992
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1034
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1208
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1337
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1506
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1507..2442
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2420..2425
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /note="hexamer: AGTAAA"
     polyA_site      2442
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /note="major polyA site"
ORIGIN      
        1 attctggcca tttgccctcc ttcccccctt cgtccgctct cattggctct gctggtaagt
       61 ggtctattcc tgcccacccc cgggtgacta gcttggccag tagtcgaccc cacccgggga
      121 ccgactctgg gggttggaga gactcttggg gccggggtcg ggcactccag ctttcttcta
      181 gccccgagct gggattccct ggcctgcgcc agctgcgtac acggcgagta caccgcacct
      241 gcccgggact tcacccgcag ctgcgagact cctccattcc cggagggctc cccacacctg
      301 ctgcggccgt gccccatctc cccgcagctg cggcgctgag cacccccatg tcgagaggct
      361 gagaccagga cagcagccct gaccaacgct ccaataggcc gggatccagc catacttcaa
      421 tggatcccag gggtatcttg aaggcatttc ccaagcggca gaaaattcat gctgatgcat
      481 catcaaaagt acttgcaaag attcctagga gggaagaggg agaagaagca gaagagtggc
      541 tgagctccct tcgggcccat gttgtgcgca ctggcattgg acgagcccgg gcagaactct
      601 ttgagaagca gattgttcag catggcggcc agctatgccc tgcccagggc ccaggtgtca
      661 ctcacattgt ggtggatgaa ggcatggact atgagcgagc cctccgcctt ctcagactac
      721 cccagctgcc cccgggtgct cagctggtga agtcagcctg gctgagcttg tgccttcagg
      781 agaggaggct ggtggatgta gctggattca gcatcttcat ccccagtagg tacttggacc
      841 atccacagcc cagcaaggca gagcaggatg cttctattcc tcctggcacc catgaggccc
      901 tgcttcagac agccctttct cctcctcctc ctcccaccag gcctgtgtct cctccccaaa
      961 aggcaaaaga ggcaccaaac acccaagccc agcccatctc tgatgatgaa gccagtgatg
     1021 gggaagaaac ccaggaggcc tgcagtatcc ctgggattgg gaagcggatg gctgagaaaa
     1081 tcatagagat cctggagagc gggcatttgc ggaagctgga ccatatcagt gagagcgtgc
     1141 ctgtcttgga gctcttctcc aacatctggg gagctgggac caagactgcc cagatgtggt
     1201 accaacaggg cttccgaagt ctggaagaca tccgcagcca ggcctccctg acaacccagc
     1261 aggccatcgg cctgaagcat tacagtgact tcctggaacg tatgcccagg gaggaggcta
     1321 cagagattga gcagacagtc cagaaagcag cccaggcctt taactctggg ctgctgtgtg
     1381 tggcatgtgg ttcataccga cggggaaagg cgacctgtgg tgatgtcgac gtgctcatca
     1441 ctcacccaga tggccggtcc caccggggta tcttcagccg cctccttgac agtcttcggc
     1501 aggaagggtt cctcacagat gacttggtga gccaagagga gaatggtcag caacagaagt
     1561 acttgggggt gtgccggctc ccagggccag ggcggcggca ccggcgcctg gacatcatcg
     1621 tggtgcccta tagcgagttt gcctgtgccc tgctctactt caccggctct gcacacttca
     1681 accgctccat gcgagccctg gccaaaacca agggcatgag tctgtcagaa catgccctca
     1741 gcactgctgt ggtccggaac acccatggct gcaaggtggg gcctggccga gtgctgccca
     1801 ctcccactga gaaggatgtc ttcaggctct taggcctccc ctaccgagaa cctgctgagc
     1861 gggactggtg acccatggct gggggtgctg aggagagccg agttggactg gctacccctc
     1921 ctggccaccc agtactccct ccagcctcag ctggctgaac ctcgccgctc caaccaccag
     1981 cttcctcagc gagcagggcc cagggctctg ggcctgaagc aagagccagc ccggctccca
     2041 gtgtctgccc ggctcccagt gtctgcccag ccctctccca gacaggagca ggctgccacc
     2101 ccttctacct caccactgcc cctcgaagaa ttttgcaaat ggccccttgc cccattttaa
     2161 gcaggagcag gtggctggtt tgaagcccca ggtatccccc ttccctgcta tgggaaaggc
     2221 caagctgctg ggtggggaca gaagctgcag gggagaggga agcagccgtg ctgtcaacat
     2281 catccggcac cctctggggt aggagaacag ccattccaca tgtgttccct ctatccgtcc
     2341 tgcttcctgg gcagctggtg gtgctgggaa tggggtgccc cagccttggt gaggacagtg
     2401 ttgggaggcc caggggccca gtaaagtgca tttgacattg aa
//
