LOCUS       NM_001174084            2781 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens DNA polymerase lambda (POLL), transcript variant 1,
            mRNA.
ACCESSION   NM_001174084
VERSION     NM_001174084.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ghazi,M., Khanna,S., Subramaniam,Y., Rengaraju,J., Sultan,F.,
            Gupta,I., Sharma,K., Chandna,S., Gokhale,R.S. and Natarajan,V.T.
  TITLE     Sustained pigmentation causes DNA damage and invokes translesion
            polymerase Polkappa for repair in melanocytes
  JOURNAL   Nucleic Acids Res 51 (19), 10451-10466 (2023)
   PUBMED   37697436
  REMARK    GeneRIF: Sustained pigmentation causes DNA damage and invokes
            translesion polymerase Polkappa for repair in melanocytes.
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Mentegari,E., Bertoletti,F., Kissova,M., Zucca,E., Galli,S.,
            Tagliavini,G., Garbelli,A., Maffia,A., Bione,S., Ferrari,E.,
            Fagagna,F.D.D., Francia,S., Sabbioneda,S., Chen,L.Y., Lingner,J.,
            Bergoglio,V., Hoffmann,J.S., Hubscher,U., Crespan,E. and Maga,G.
  TITLE     A Role for Human DNA Polymerase lambda in Alternative Lengthening
            of Telomeres
  JOURNAL   Int J Mol Sci 22 (5), 2365 (2021)
   PUBMED   33673424
  REMARK    GeneRIF: A Role for Human DNA Polymerase lambda in Alternative
            Lengthening of Telomeres.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2781)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2781)
  AUTHORS   Craxton,A., Munnur,D., Jukes-Jones,R., Skalka,G., Langlais,C.,
            Cain,K. and Malewicz,M.
  TITLE     PAXX and its paralogs synergistically direct DNA polymerase lambda
            activity in DNA repair
  JOURNAL   Nat Commun 9 (1), 3877 (2018)
   PUBMED   30250067
  REMARK    GeneRIF: PAXX, XLF and XRCC4 synergise in the efficient DNA
            double-strand breaks recruitment, substrate recognition and
            stimulation of Pol lambda enzymatic activity during nonhomologous
            end joining DNA repair.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2781)
  AUTHORS   van Loon,B., Hubscher,U. and Maga,G.
  TITLE     Living on the Edge: DNA Polymerase Lambda between Genome Stability
            and Mutagenesis
  JOURNAL   Chem Res Toxicol 30 (11), 1936-1941 (2017)
   PUBMED   28841305
  REMARK    GeneRIF: When mutated or deregulated, DNA polymerase lambda can
            also be a source of genetic instability. Its multiple roles in DNA
            damage tolerance and its ability in promoting tumor progression
            make it also a possible target for novel anticancer approaches.
            [review]
            Review article
REFERENCE   6  (bases 1 to 2781)
  AUTHORS   Haracska,L., Unk,I., Johnson,R.E., Phillips,B.B., Hurwitz,J.,
            Prakash,L. and Prakash,S.
  TITLE     Stimulation of DNA synthesis activity of human DNA polymerase kappa
            by PCNA
  JOURNAL   Mol Cell Biol 22 (3), 784-791 (2002)
   PUBMED   11784855
REFERENCE   7  (bases 1 to 2781)
  AUTHORS   Garcia-Diaz,M., Bebenek,K., Kunkel,T.A. and Blanco,L.
  TITLE     Identification of an intrinsic 5'-deoxyribose-5-phosphate lyase
            activity in human DNA polymerase lambda: a possible role in base
            excision repair
  JOURNAL   J Biol Chem 276 (37), 34659-34663 (2001)
   PUBMED   11457865
REFERENCE   8  (bases 1 to 2781)
  AUTHORS   Nagasawa,K., Kitamura,K., Yasui,A., Nimura,Y., Ikeda,K., Hirai,M.,
            Matsukage,A. and Nakanishi,M.
  TITLE     Identification and characterization of human DNA polymerase beta 2,
            a DNA polymerase beta -related enzyme
  JOURNAL   J Biol Chem 275 (40), 31233-31238 (2000)
   PUBMED   10887191
REFERENCE   9  (bases 1 to 2781)
  AUTHORS   Aoufouchi,S., Flatter,E., Dahan,A., Faili,A., Bertocci,B.,
            Storck,S., Delbos,F., Cocea,L., Gupta,N., Weill,J.C. and
            Reynaud,C.A.
  TITLE     Two novel human and mouse DNA polymerases of the polX family
  JOURNAL   Nucleic Acids Res 28 (18), 3684-3693 (2000)
   PUBMED   10982892
REFERENCE   10 (bases 1 to 2781)
  AUTHORS   Garcia-Diaz,M., Dominguez,O., Lopez-Fernandez,L.A., de Lera,L.T.,
            Saniger,M.L., Ruiz,J.F., Parraga,M., Garcia-Ortiz,M.J.,
            Kirchhoff,T., del Mazo,J., Bernad,A. and Blanco,L.
  TITLE     DNA polymerase lambda (Pol lambda), a novel eukaryotic DNA
            polymerase with a potential role in meiosis
  JOURNAL   J Mol Biol 301 (4), 851-867 (2000)
   PUBMED   10966791
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC296090.1, BC068529.1 and
            AJ131890.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001174084.1.
            
            Summary: This gene encodes a DNA polymerase. DNA polymerases
            catalyze DNA-template-directed extension of the 3'-end of a DNA
            strand. This particular polymerase, which is a member of the X
            family of DNA polymerases, likely plays a role in non-homologous
            end joining and other DNA repair processes. Alternatively spliced
            transcript variants have been described. [provided by RefSeq, Mar
            2010].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a). Both variants 1 and
            2 encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC068529.1, SRR1163655.400757.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370162.8/ ENSP00000359181.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-98                DC296090.1         6-103
            99-1374             BC068529.1         1-1276
            1375-1375           AJ131890.1         1264-1264
            1376-2781           BC068529.1         1278-2683
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q24.32"
     gene            1..2781
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /note="DNA polymerase lambda"
                     /db_xref="GeneID:27343"
                     /db_xref="HGNC:HGNC:9184"
                     /db_xref="MIM:606343"
     exon            1..436
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     exon            437..597
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     CDS             483..2210
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /EC_number="2.7.7.7"
                     /note="isoform a is encoded by transcript variant 1; DNA
                     polymerase beta-2; polymerase (DNA) lambda; DNA polymerase
                     beta-N; polymerase (DNA directed), lambda; DNA polymerase
                     kappa"
                     /codon_start=1
                     /product="DNA polymerase lambda isoform a"
                     /protein_id="NP_001167555.1"
                     /db_xref="CCDS:CCDS7513.1"
                     /db_xref="GeneID:27343"
                     /db_xref="HGNC:HGNC:9184"
                     /db_xref="MIM:606343"
                     /translation="MDPRGILKAFPKRQKIHADASSKVLAKIPRREEGEEAEEWLSSL
                     RAHVVRTGIGRARAELFEKQIVQHGGQLCPAQGPGVTHIVVDEGMDYERALRLLRLPQ
                     LPPGAQLVKSAWLSLCLQERRLVDVAGFSIFIPSRYLDHPQPSKAEQDASIPPGTHEA
                     LLQTALSPPPPPTRPVSPPQKAKEAPNTQAQPISDDEASDGEETQVSAADLEALISGH
                     YPTSLEGDCEPSPAPAVLDKWVCAQPSSQKATNHNLHITEKLEVLAKAYSVQGDKWRA
                     LGYAKAINALKSFHKPVTSYQEACSIPGIGKRMAEKIIEILESGHLRKLDHISESVPV
                     LELFSNIWGAGTKTAQMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEA
                     TEIEQTVQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDS
                     LRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLYFTG
                     SAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGRVLPTPTEKDVFRLLGLP
                     YREPAERDW"
     exon            598..892
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1055
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1373
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1547
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1676
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1845
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..2781
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2759..2764
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /note="hexamer: AGTAAA"
     polyA_site      2781
                     /gene="POLL"
                     /gene_synonym="BETAN; POLKAPPA"
                     /note="major polyA site"
ORIGIN      
        1 attctggcca tttgccctcc ttcccccctt cgtccgctct cattggctct gctggtaagt
       61 ggtctattcc tgcccacccc cgggtgacta gcttggccag tagtcgaccc cacccgggga
      121 ccgactctgg gggttggaga gactcttggg gccggggtcg ggcactccag ctttcttcta
      181 gccccgagct gggattccct ggcctgcgcc agctgcgtac acggcgagta caccgcacct
      241 gcccgggact tcacccgcag ctgcgagact cctccattcc cggagggctc cccacacctg
      301 ctgcggccgt gccccatctc cccgcagctg cggcgctgag cacccccatg tcgagaggct
      361 gagaccagga caggtgcagg gcgttcccac tcacccccga aagtcctcct ccttcctctg
      421 cgagtctgtg ttggagcagc cctgaccaac gctccaatag gccgggatcc agccatactt
      481 caatggatcc caggggtatc ttgaaggcat ttcccaagcg gcagaaaatt catgctgatg
      541 catcatcaaa agtacttgca aagattccta ggagggaaga gggagaagaa gcagaagagt
      601 ggctgagctc ccttcgggcc catgttgtgc gcactggcat tggacgagcc cgggcagaac
      661 tctttgagaa gcagattgtt cagcatggcg gccagctatg ccctgcccag ggcccaggtg
      721 tcactcacat tgtggtggat gaaggcatgg actatgagcg agccctccgc cttctcagac
      781 taccccagct gcccccgggt gctcagctgg tgaagtcagc ctggctgagc ttgtgccttc
      841 aggagaggag gctggtggat gtagctggat tcagcatctt catccccagt aggtacttgg
      901 accatccaca gcccagcaag gcagagcagg atgcttctat tcctcctggc acccatgagg
      961 ccctgcttca gacagccctt tctcctcctc ctcctcccac caggcctgtg tctcctcccc
     1021 aaaaggcaaa agaggcacca aacacccaag cccagcccat ctctgatgat gaagccagtg
     1081 atggggaaga aacccaggtt agtgcagctg atctggaagc cctcatcagt ggccactacc
     1141 ccacctccct tgagggagat tgtgagccta gcccagcccc tgctgtcctg gataagtggg
     1201 tctgtgcaca gccctcaagc cagaaggcga ccaatcacaa cctccatatc acagagaagc
     1261 tggaagttct ggccaaagcc tacagtgttc agggagacaa gtggagggcc ctgggctatg
     1321 ccaaggccat caatgccctc aagagcttcc ataagcctgt cacctcgtac caggaggcct
     1381 gcagtatccc tgggattggg aagcggatgg ctgagaaaat catagagatc ctggagagcg
     1441 ggcatttgcg gaagctggac catatcagtg agagcgtgcc tgtcttggag ctcttctcca
     1501 acatctgggg agctgggacc aagactgccc agatgtggta ccaacagggc ttccgaagtc
     1561 tggaagacat ccgcagccag gcctccctga caacccagca ggccatcggc ctgaagcatt
     1621 acagtgactt cctggaacgt atgcccaggg aggaggctac agagattgag cagacagtcc
     1681 agaaagcagc ccaggccttt aactctgggc tgctgtgtgt ggcatgtggt tcataccgac
     1741 ggggaaaggc gacctgtggt gatgtcgacg tgctcatcac tcacccagat ggccggtccc
     1801 accggggtat cttcagccgc ctccttgaca gtcttcggca ggaagggttc ctcacagatg
     1861 acttggtgag ccaagaggag aatggtcagc aacagaagta cttgggggtg tgccggctcc
     1921 cagggccagg gcggcggcac cggcgcctgg acatcatcgt ggtgccctat agcgagtttg
     1981 cctgtgccct gctctacttc accggctctg cacacttcaa ccgctccatg cgagccctgg
     2041 ccaaaaccaa gggcatgagt ctgtcagaac atgccctcag cactgctgtg gtccggaaca
     2101 cccatggctg caaggtgggg cctggccgag tgctgcccac tcccactgag aaggatgtct
     2161 tcaggctctt aggcctcccc taccgagaac ctgctgagcg ggactggtga cccatggctg
     2221 ggggtgctga ggagagccga gttggactgg ctacccctcc tggccaccca gtactccctc
     2281 cagcctcagc tggctgaacc tcgccgctcc aaccaccagc ttcctcagcg agcagggccc
     2341 agggctctgg gcctgaagca agagccagcc cggctcccag tgtctgcccg gctcccagtg
     2401 tctgcccagc cctctcccag acaggagcag gctgccaccc cttctacctc accactgccc
     2461 ctcgaagaat tttgcaaatg gccccttgcc ccattttaag caggagcagg tggctggttt
     2521 gaagccccag gtatccccct tccctgctat gggaaaggcc aagctgctgg gtggggacag
     2581 aagctgcagg ggagagggaa gcagccgtgc tgtcaacatc atccggcacc ctctggggta
     2641 ggagaacagc cattccacat gtgttccctc tatccgtcct gcttcctggg cagctggtgg
     2701 tgctgggaat ggggtgcccc agccttggtg aggacagtgt tgggaggccc aggggcccag
     2761 taaagtgcat ttgacattga a
//
