LOCUS       NM_001395899           14470 bp    mRNA    linear   PRI 13-DEC-2024
DEFINITION  Homo sapiens DNA polymerase kappa (POLK), transcript variant 14,
            mRNA.
ACCESSION   NM_001395899
VERSION     NM_001395899.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14470)
  AUTHORS   Ribeiro,D.L., Latancia,M.T., de Souza,I., Ariwoola,A.A., Mendes,D.,
            Rocha,C.R.R., Lengert,A.V.H. and Menck,C.F.M.
  TITLE     Temozolomide resistance mechanisms: unveiling the role of
            translesion DNA polymerase kappa in glioblastoma spheroids in vitro
  JOURNAL   Biosci Rep 44 (5) (2024)
   PUBMED   38717250
  REMARK    GeneRIF: Temozolomide resistance mechanisms: unveiling the role of
            translesion DNA polymerase kappa in glioblastoma spheroids in
            vitro.
REFERENCE   2  (bases 1 to 14470)
  AUTHORS   Maghsoud,Y., Roy,A., Leddin,E.M. and Cisneros,G.A.
  TITLE     Effects of the Y432S Cancer-Associated Variant on the Reaction
            Mechanism of Human DNA Polymerase kappa
  JOURNAL   J Chem Inf Model 64 (10), 4231-4249 (2024)
   PUBMED   38717969
  REMARK    GeneRIF: Effects of the Y432S Cancer-Associated Variant on the
            Reaction Mechanism of Human DNA Polymerase kappa.
REFERENCE   3  (bases 1 to 14470)
  AUTHORS   Pathira Kankanamge,L.S., Mora,A., Ondrechen,M.J. and Beuning,P.J.
  TITLE     Biochemical Activity of 17 Cancer-Associated Variants of DNA
            Polymerase Kappa Predicted by Electrostatic Properties
  JOURNAL   Chem Res Toxicol 36 (11), 1789-1803 (2023)
   PUBMED   37883788
  REMARK    GeneRIF: Biochemical Activity of 17 Cancer-Associated Variants of
            DNA Polymerase Kappa Predicted by Electrostatic Properties.
REFERENCE   4  (bases 1 to 14470)
  AUTHORS   Yudkina,A.V. and Zharkov,D.O.
  TITLE     Miscoding and DNA Polymerase Stalling by Methoxyamine-Adducted
            Abasic Sites
  JOURNAL   Chem Res Toxicol 35 (2), 303-314 (2022)
   PUBMED   35089032
  REMARK    GeneRIF: Miscoding and DNA Polymerase Stalling by
            Methoxyamine-Adducted Abasic Sites.
REFERENCE   5  (bases 1 to 14470)
  AUTHORS   Lancey,C., Tehseen,M., Bakshi,S., Percival,M., Takahashi,M.,
            Sobhy,M.A., Raducanu,V.S., Blair,K., Muskett,F.W., Ragan,T.J.,
            Crehuet,R., Hamdan,S.M. and De Biasio,A.
  TITLE     Cryo-EM structure of human Pol kappa bound to DNA and
            mono-ubiquitylated PCNA
  JOURNAL   Nat Commun 12 (1), 6095 (2021)
   PUBMED   34667155
  REMARK    GeneRIF: Cryo-EM structure of human Pol kappa bound to DNA and
            mono-ubiquitylated PCNA.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 14470)
  AUTHORS   Gerlach,V.L., Feaver,W.J., Fischhaber,P.L. and Friedberg,E.C.
  TITLE     Purification and characterization of pol kappa, a DNA polymerase
            encoded by the human DINB1 gene
  JOURNAL   J Biol Chem 276 (1), 92-98 (2001)
   PUBMED   11024016
REFERENCE   7  (bases 1 to 14470)
  AUTHORS   Ohashi,E., Ogi,T., Kusumoto,R., Iwai,S., Masutani,C., Hanaoka,F.
            and Ohmori,H.
  TITLE     Error-prone bypass of certain DNA lesions by the human DNA
            polymerase kappa
  JOURNAL   Genes Dev 14 (13), 1589-1594 (2000)
   PUBMED   10887153
REFERENCE   8  (bases 1 to 14470)
  AUTHORS   Johnson,R.E., Prakash,S. and Prakash,L.
  TITLE     The human DINB1 gene encodes the DNA polymerase Poltheta
  JOURNAL   Proc Natl Acad Sci U S A 97 (8), 3838-3843 (2000)
   PUBMED   10760255
REFERENCE   9  (bases 1 to 14470)
  AUTHORS   Ogi,T., Kato,T. Jr., Kato,T. and Ohmori,H.
  TITLE     Mutation enhancement by DINB1, a mammalian homologue of the
            Escherichia coli mutagenesis protein dinB
  JOURNAL   Genes Cells 4 (11), 607-618 (1999)
   PUBMED   10620008
REFERENCE   10 (bases 1 to 14470)
  AUTHORS   Gerlach,V.L., Aravind,L., Gotway,G., Schultz,R.A., Koonin,E.V. and
            Friedberg,E.C.
  TITLE     Human and mouse homologs of Escherichia coli DinB (DNA polymerase
            IV), members of the UmuC/DinB superfamily
  JOURNAL   Proc Natl Acad Sci U S A 96 (21), 11922-11927 (1999)
   PUBMED   10518552
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC116341.2, AC026424.5 and
            AC010245.4.
            
            Summary: This gene encodes a member of the DNA polymerase type-Y
            family of proteins. The encoded protein is a specialized DNA
            polymerase that catalyzes translesion DNA synthesis, which allows
            DNA replication in the presence of DNA lesions. Human cell lines
            lacking a functional copy of this gene exhibit impaired genome
            integrity and enhanced susceptibility to oxidative damage.
            Mutations in this gene that impair enzyme activity may be
            associated with prostate cancer in human patients. [provided by
            RefSeq, Sep 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA2144120,
                              SAMEA2145240 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AC116341.2         23623-23646
            25-172              AC026424.5         92059-92206         c
            173-292             AC026424.5         86625-86744         c
            293-424             AC010245.4         7988-8119
            425-578             AC010245.4         11030-11183
            579-818             AC010245.4         15459-15698
            819-943             AC010245.4         17543-17667
            944-1110            AC010245.4         19010-19176
            1111-1143           AC010245.4         21276-21308
            1144-1240           AC010245.4         24594-24690
            1241-1412           AC010245.4         28128-28299
            1413-2369           AC010245.4         30472-31428
            2370-2412           AC010245.4         31997-32039
            2413-14470          AC010245.4         32184-44241
FEATURES             Location/Qualifiers
     source          1..14470
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q13.3"
     gene            1..14470
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /note="DNA polymerase kappa"
                     /db_xref="GeneID:51426"
                     /db_xref="HGNC:HGNC:9183"
                     /db_xref="MIM:605650"
     exon            1..24
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /inference="alignment:Splign"
     exon            25..172
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /inference="alignment:Splign"
     CDS             38..2497
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /EC_number="2.7.7.7"
                     /note="isoform 7 is encoded by transcript variant 14;
                     polymerase (DNA directed) kappa"
                     /codon_start=1
                     /product="DNA polymerase kappa isoform 7"
                     /protein_id="NP_001382828.1"
                     /db_xref="GeneID:51426"
                     /db_xref="HGNC:HGNC:9183"
                     /db_xref="MIM:605650"
                     /translation="MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEAT
                     KGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQSTSNYHARRFGVRAAMP
                     GFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEER
                     QNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISPLLFEESPSDVQPPGDPFQV
                     NFEEQNNPQILQNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKN
                     KPNGQYQILPNRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSL
                     LFSETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELA
                     QDLQKERLKGRTVTIKLKNVNFEVKTRASTVSSVVSTAEEIFAIAKELLKTEIDADFP
                     HPLRLRLMGVRISSFPNEEDRKHQQRSIIGFLQAGNQALSATECTLEKTDKDKFVKPL
                     EMSHKKSFFDKKRSERKWSHQDTFKCEAVNKQSFQTSQPFQVLKKKMNENLEISENSD
                     DCQILTCPVCFRAQGCISLEALNKHVDECLDGPSISENFKMFSCSHVSATKVNKKENV
                     PASSLCEKQDYEAHPKIKEISSVDCIALVDTIDNSSKAESIDALSNKHSKEECSSLPS
                     KSFNIEHCHQNSSSTVSLENEDVGSFRQEYRQPYLCEVKTGQALVCPVCNVEQKTSDL
                     TLFNVHVDVCLNKSFIQELRKDKFNPVNQPKESSRSTGSSSGVQKAVTRTKRPGLMTK
                     YSTSKKIKPNNPKHTLDIFFK"
     exon            173..292
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /inference="alignment:Splign"
     exon            293..424
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /inference="alignment:Splign"
     exon            425..578
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /inference="alignment:Splign"
     exon            579..818
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /inference="alignment:Splign"
     exon            819..943
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /inference="alignment:Splign"
     exon            944..1110
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /inference="alignment:Splign"
     exon            1111..1143
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /inference="alignment:Splign"
     exon            1144..1240
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /inference="alignment:Splign"
     exon            1241..1412
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /inference="alignment:Splign"
     exon            1413..2369
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /inference="alignment:Splign"
     exon            2370..2412
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /inference="alignment:Splign"
     exon            2413..14470
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /inference="alignment:Splign"
     regulatory      2493..2498
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /note="hexamer: AGTAAA"
     polyA_site      2519
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
     regulatory      4272..4277
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /note="hexamer: AATAAA"
     polyA_site      4295
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /note="major polyA site"
     regulatory      5599..5604
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /note="hexamer: AATAAA"
     polyA_site      5623
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
     regulatory      14446..14451
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
                     /note="hexamer: AATAAA"
     polyA_site      14470
                     /gene="POLK"
                     /gene_synonym="DINB1; DINP; POLQ"
ORIGIN      
        1 gtagtcgcga tcctgaggta acggataagt ttataccatg gatagcacaa aggagaagtg
       61 tgacagttac aaagatgatc ttctgcttag gatgggactt aatgataata aagcaggaat
      121 ggaaggatta gataaagaga aaattaacaa aattataatg gaagccacga aggggtccag
      181 attttatgga aatgagctca agaaagaaaa gcaagtcaac caacgaattg aaaatatgat
      241 gcaacaaaaa gctcaaatca ccagccaaca gctaagaaaa gcacaattac agtctacttc
      301 aaattaccat gcaaggagat ttggtgttcg tgcagccatg ccaggattta ttgctaagag
      361 gctgtgccca caacttataa tagtgccccc caactttgac aaataccgag ctgtgagtaa
      421 agaggttaag gaaatacttg ctgattatga tcccaatttt atggccatga gtcttgatga
      481 agcctacttg aatataacaa agcacttaga agaaagacaa aattggcctg aggataaaag
      541 aaggtatttc atcaaaatgg gaagctctgt agaaaatgat aatccaggaa aggaagttaa
      601 taaactgagt gagcatgaac gatccatctc tccactactt tttgaagaga gtccttctga
      661 tgtgcagccc ccaggagatc ctttccaagt gaactttgaa gaacaaaaca atcctcaaat
      721 actccaaaac tcagttgttt ttggaacatc agcccaggaa gtggtaaagg aaattcgttt
      781 cagaattgag cagaaaacaa cactgacagc cagtgcaggc attgccccaa atacaatgtt
      841 agcaaaagta tgcagtgata agaataaacc aaatggacaa taccaaattc ttcccaatag
      901 acaagctgtg atggacttca tcaaggattt acccattaga aaggtttctg gaataggaaa
      961 agttacagag aaaatgttaa aggcccttgg aattattaca tgtacagaac tttaccaaca
     1021 gagggcattg ctttctctcc ttttctctga aacatcttgg cattatttcc ttcatatctc
     1081 cttgggtcta ggttcaacac acctgacgag ggatggagag aggaaaagta tgagcgttga
     1141 gaggacattc agtgagataa ataaagcgga agagcaatac agcctatgtc aagaactttg
     1201 cagtgagctt gctcaggatc tacagaaaga aagacttaag ggtagaactg ttaccattaa
     1261 gttgaagaat gtgaattttg aagtaaaaac tcgtgcatct acagtttcat ctgttgtttc
     1321 tactgcagaa gaaatatttg ccattgctaa ggaattgcta aaaacagaaa ttgatgctga
     1381 ttttccacat cccttgagat taaggcttat gggtgttcgg atatctagtt ttcccaatga
     1441 agaggacagg aaacaccaac aaaggagcat tattggcttt ttacaggctg gaaaccaagc
     1501 cctgtcagcc actgagtgta cattagagaa aactgacaaa gataagtttg taaaacctct
     1561 agaaatgtct cataagaaga gtttctttga taaaaaacga tcagaaagga aatggagtca
     1621 ccaagataca tttaaatgtg aagccgtgaa taaacaaagt ttccagacat cacaaccatt
     1681 ccaagtttta aagaagaaga tgaatgagaa tttggaaata tcagagaatt cagatgactg
     1741 tcagatactt acctgtcctg tttgctttag ggctcaaggg tgcatcagtc tggaagcctt
     1801 gaataaacat gtagatgaat gtcttgatgg accttcaatc agtgaaaact ttaaaatgtt
     1861 ctcgtgttca catgtttctg ctaccaaagt taacaagaaa gaaaatgttc ctgcttcttc
     1921 actttgtgag aagcaagatt atgaagccca tccaaaaatt aaagaaatat cttcagtaga
     1981 ttgtatagct ttagtagata ctatagataa ctcatctaaa gcagaaagca tagatgcttt
     2041 aagtaataag catagcaagg aagaatgttc tagtctccca agcaagtctt ttaatattga
     2101 acactgtcat cagaattctt cttctactgt ttcattggaa aacgaagatg ttggatcatt
     2161 tagacaagaa taccgccagc cttacttatg tgaagtgaaa acaggccaag ctctagtttg
     2221 tcctgtttgt aacgtagaac aaaagacttc agatctaacc ctgttcaatg tgcatgtgga
     2281 tgtttgctta aataaaagtt ttatccaaga attaagaaag gataaattta acccagttaa
     2341 tcaacccaaa gaaagctcca gaagtactgg tagctcaagt ggagtacaga aggctgtaac
     2401 aagaacaaaa aggccaggat tgatgacaaa gtactcaaca tcaaagaaaa taaaaccaaa
     2461 caatcccaaa catacccttg atatattttt taagtaaaca ttgaacattt tatcattaat
     2521 ttttaattga aactagttat tttataatca atgaatttgt tctttctgat tttaagtttg
     2581 cagatttatt tagtgaaggc aagtgcaata atccttcctc agatgatgtt tgcttttcta
     2641 agatacatat actgattctg tgtatctttt ttataaccat gagaatttta cttccattat
     2701 acatcaattg gaaatcaatc ctgttaagag ataattctta aaagggaaat taggaatggg
     2761 ataagaaggt gattttttta ttatttttat actgaatata aaaacatttg taagggctct
     2821 caaagattca cacatgccta tattatcata agaatttttc agcacttaac tactttgttg
     2881 gcattgatcc tagtgtcttt aaatacttca tgagcattca taattaaaat ttatcttaag
     2941 ttctatgaag agtattaatg taactagcat aagtggtttt cttcaggaaa ataaatatca
     3001 cagtattatc tgtgttaaaa tggtttttgc ctaaaatata atttttaatt tggcttttct
     3061 tatttaaaat tccattatct tatgaataag cacttgaatc agtttttaaa atatttagtc
     3121 taagatgatt caaagtagtt ttattttaat acaggacttt taaatggcag tatttcattt
     3181 cttgtcaatt atgttggtac tttccacaaa tctataaaga aggataaatt gtaccatcat
     3241 tttattataa tcctcaagag aaaatgtgta attcaaaaga ttaatgtgta ttaaaacaca
     3301 ttatgtatct tagttacatt tctatcagta cttttattaa tatttgtgaa agaagacagc
     3361 ttaatagtag ttagcttaag tagtttctcc aagtactttt gtgctatcaa tgagttcttc
     3421 tcaaaaaata attagttagg ccaggcacaa tggctcacac ctgtaatgcc agccctttgg
     3481 gaggccgaat gggcagatca cttgaggtca ggagtttgag accagcctcg ccaacatggt
     3541 gagaccctgt ctctactaaa acgataaaaa aaaaaaaaaa attagccagg cttggtggca
     3601 cacgcctgta atcccagcta ctcagatggc tgaggcagga gaactacttg aacctgggag
     3661 gtcaaagctg cagtcagcca agatcttgcc actgtactcc agcctgaaga gcgagactct
     3721 gtctcaataa tataataata gttattattt aattgcaaca tgaagttgga agccattttc
     3781 tgttacttct ttaaggagct aaatctttta tatttttgtt ttctctcccc ttgcgtttca
     3841 tattttagct tatgatctaa atatatattt tgtatattat aatattttag gaatagctta
     3901 attaagaact cacagacttt gacaaagtgg taactagact tttaattact agattcttca
     3961 aattaggctt aatgcctgct agcatgccaa tccaatccca tgactgatgt tattatacac
     4021 tatggcaaat acaaagttgt agtattatgg ttcttgatgg tgtgatttac ttatgtaaat
     4081 tatgtacaaa tttaagttca atgaaacact gatttaatgt actgaaaggt aaatagtacc
     4141 atagtgaatt attttctgtc ttgccttagg tttattttta ttatcttcaa tattagaata
     4201 aatgctaaat tattttcatt taaggattta tgtagttgaa tattatcatt tagtaatatt
     4261 taatcatttg aaataaaatt taaagcttta attgatctaa tagagatggt tttctacttt
     4321 tccactcttc ctccctctta tgccttcact tttgttttct gtgcccagaa ttttcttcac
     4381 attctttact acctgttttg tctattatat ccaacccatt ttctttttat tataattttg
     4441 atttggggca tttgaagaaa atacagcaaa actacaagaa ctggtaataa gcatgcataa
     4501 tctcacctac ttagtgaacc taatataaac ctaatataaa gaaccaccgt atttgcaata
     4561 tatataactg gtgaaaaact agtgtccaga atatataaat gatccctatg aatcagtaag
     4621 aaaaagacag tcaatagaaa tttggacaaa agactttata tggcatttca agtgaaagga
     4681 aatccagatg accaagaaac atgaaaatat tcccagccat attaatattt gagaagtaaa
     4741 aaataaaact acaaggaggt attacatctt taccttattt gcaaaaatga aatgatctaa
     4801 tgatactaag ttttggtgat gatgtcagct cttacattga aaacaatcac tgtggaaaaa
     4861 tttgtccctc agtaaattta aagataaaca tgctcaaaac ctagcaattg cactctgaag
     4921 tatacatctt tgcaggttat ctttcattac agttattttt ttccctgtca ggaccctaac
     4981 taatacaatg ccttgggaaa actaatgaac atgtatccta ggaagcatgt acaagaaagt
     5041 tcttcccagt caggaaggtg gccaagaaaa aaatttttta atgttcatag cagcactgca
     5101 atatccaaaa gttagaaatg aaccaaatat tcagcaatgg tagaatggtt aaattgtacc
     5161 atggcattta tattgaaaca gtagaattct acacagtggt gaaaatgaag gaaatagcta
     5221 accacaacat agatgaatat cacagacata gcatagagca aaagaagcaa atcacagaag
     5281 gatgccatca acatgattcc atttgtataa agtttaaaac agtataaact gaacaactat
     5341 attgttttaa gggtatatag ttatatatat gagatacaac tgtaaagaaa agcaagtgtt
     5401 ttgctgtccc cagaaccaga ataatgattt cctctgtgga gaagtacagc agtgtgattg
     5461 gggagtggca cctggtgctt ctgggatgct ggcaatgttc tatttcttgt cttgggtggt
     5521 gattgcatgg atgttcactt ttaaaatact ctttatacat atatatttta tatattcttg
     5581 ttcatatata acctttataa taaagagaaa aaaatggcaa aaactgcttt cgttaacaag
     5641 atgatatttt cctccaactc tgcaggctag ctaatttcaa tcgagaaaaa aatacatttt
     5701 tttcttctag aaccttgctg aacacagcaa gaaagagcca tcccatgcaa aatttttagt
     5761 ttttctccta gcatttcccc taaggctaca gcctcaatca gtatttggtc ttctttccaa
     5821 agacagctgt tgacagttag accaaatatt tttttgccgt ttgtgatggt taatattgag
     5881 tgtcaacttg attggattaa atgatgcaaa atattgttcc tgggtgtgtc tgtgagggta
     5941 ttgccaaaga agattaacat ttgagtcagt gggctcaaga agcagatcca ccctcaatct
     6001 ggttgtgcac catctaatca actgccagca tagctaggat aaaagcaggc agaggaaggt
     6061 ggaaggacta cactggctaa gtcttctggc ctccgtcttt ctcctgtgct gcatgtcttc
     6121 tgcccttgaa catcagactc caagttcttc agcttttgga ctcttggact tccaccagtg
     6181 atttgccagg gactatcagg ccttcagcca cagactgaag ggctgcacca tctgcttgtc
     6241 tgcttttgag gttttgggac tcagactggc ttcctggctt ctcagcttgc agactgtggg
     6301 acttcacctt gtgattgtgt gagtcaattt tcctaagaaa ctccccttca tatatacatc
     6361 tattagttct gtccctctag aggaccctaa tacaccacca aacaagtcac cagttttcca
     6421 gtctgtggta tttgtgtccc cactccctaa attcttctct ccaaccctaa gtcaatttgt
     6481 tatattaggc tttgttattt gcagtaccct atttctgtat cagttcagtc acaagcttcc
     6541 tggtgcctct tccttgttga gtcacatggg gacatgctta attctcccag aaatgtgtga
     6601 cagcatgtgc aaaatattgc caaccaagga agctcaacca atccttggtg tccaaggtgt
     6661 ttactggaag tcagtcacat aggcatgcat cacccatgtg actgacctta cccgttgagt
     6721 ctcaagaccc caggggtgta gtgatacctc atggcccagg gcttcagcca ttcaaaaaca
     6781 gacattcgcc atgattcata ctgttagcat aaactatctc gtcaaactaa caaaatgcag
     6841 cccaaggcct caggcatact agaacactat tatcaaactg gaaattccac gagctcaggt
     6901 catttcccag gagctagtca aggaagggcc agtacgtgaa gacacctctc tttgaaaagt
     6961 gcagggctgg agtaacctga ggctgctagg ttaacccttt actgcattaa tgtcaaatta
     7021 ttagaattgt attcaaataa aatataaaaa agaaagatca cttagagtaa ctttttcctg
     7081 cctcacttga ataatgcaga gacacttcaa attcgtaaca tgtttagggg gtctcaagga
     7141 ggatgggcta tgcgttttga atatatgcat ataaaacaac aaatgacata ggcctaataa
     7201 tttctttgca tgtgtataat cctctcttca atgtaattca aaggtctctt ttttaatatc
     7261 agtctttaca ttgtccctgt aatatagagc tatacacctt tcctcaatct tttgtgatag
     7321 taggaaaagt agcattataa gaagtattta taaatttaat tcactccacc agaaatgttt
     7381 tattttaaag ttagcatggg acttgtggta tccgcaaggt cagtgtcccc actgaagaag
     7441 tggccttcta gagtaagaag tttcagcatc aacatgggta atggaagcca tataacagaa
     7501 cttaagaagg aactttgtgc taagaaaatg tagacagtgg ttataggcat atatgtaaga
     7561 aggctgtaag ttaaaggcaa gagagatgta gcaattaata ggagatattt tttctgccat
     7621 cattaacaaa tgtatttgtt tactactcct tactttttaa ctcatccacc ttgtatccac
     7681 tgctacctct gccaaccctg tatctgttac tgaattctgt ctactcttgt agggcctctt
     7741 gaaaatgaaa atatccattt gtttccatct cttttgtcaa gcccttatca gttgtctaaa
     7801 ctcttgactt tccaactgat ttgtcagttt tccctgctct aaaccatctt atgcattttt
     7861 tacagattag ctataaacag cattcccata atagcactct catgtcaaaa aaaaaaaaaa
     7921 agcagttttc aaaagctctc aactgtctcc aagatttagc agtggaatga gtaggaaaag
     7981 ggagtgattt ttagtgtatg attatttatg tggtgtaaat gctcccacca tgacagattt
     8041 caagttatgt gatgtcagcc agttcacaaa attcctgaaa ctttaacagc tcttgcaagc
     8101 caatacaagc tagctcctat atatcactgt ccatgatgca ctctaaacac tggcttggca
     8161 ttcagggttt cctgaacaga ccaagctgtt tctcctttct ggcatctgtg ctccattgtt
     8221 ccctctgccc agaatatgct tcctgaatgc cccttttctc tctcccacct acataattac
     8281 tcatctttca actcagctca agggccacct tcagaaaagt gttccttgcc ctctggaaaa
     8341 attgatcagt cactattttt tgctcctgtt tccctacaaa gaccctgaaa cattctttgc
     8401 atggcccccg atgttggttt ctccctacca gattgtaggc cccaagagat cctgggctat
     8461 ctttcactta tctctgaatt ccaagtatga agtgtatggc ctggcacaca gtagatacgg
     8521 agagtcattt gttgacatgt aaatgaataa atagtatgtg tccaaaagtg tttactacta
     8581 tcttattgtt tttatctggg aagacagaat ggtggctaag ggctgctgtc acactgggtc
     8641 ctaaagagtg gacattccag aaagagacaa ttgcatttat ttccctttat tagataatgg
     8701 cttgctttat acatgtttta ttattatact gtgtcgtttt ggagtggctt gggagtattt
     8761 ggaagaaggc ttaaaatatg actattaacc tggggctaaa aaaaactgtt tttgaggaaa
     8821 tatttcattt aaaaaaatca tacctggtgt acctggtgct gaactgttgt ttttagagac
     8881 aaggtctcac cgtcacccag gctgaaatac attgtcatga tcataactca ctgcagcctg
     8941 gaactcttgg gatcaagatc ctctcatcgc agcttcccaa gtagctggga ctacaggctg
     9001 gtgccatgat gcccagctaa gtttttgtat ttttttgttg agataggatc ttgctatgtg
     9061 gcctagattg gtcttgaact cctggcttca agcattcctc cttcctcagt ctcccaaagc
     9121 tctgggatta acaggcatca gccaccatgc ctggccctgg tactgaattt tctttgaagt
     9181 aaggttccat tttgaaaagc agtgatatat ttgaacacac tttgtgttgg taggatttta
     9241 gtaaacctca cagagaggtg atgcttattt ccctggagaa aaaactgttc tttaattgta
     9301 taattaagga ctaatcgctt ttgctttgta atacataact gaattaagtt gttcaagaaa
     9361 aactcaaatg acatcatggt gactattggc aacatgattc catttattaa gcaaaccata
     9421 atattgaact gttcccctta ccagtacgag gtaattgtga tagtcttact ttgtaactgc
     9481 tgctgtctac agaatgctca gggagattaa agataattca gaggcaaatg gtactaaccc
     9541 ttctaacctc agcctgtaaa gtgtagtatg atttcaaact ctttcctgta tacacataca
     9601 cacacacaca cacacacaca cacacacaca cacacactac ctctgccaac cacataccta
     9661 tcattgagtt ctgtctactc ttgtagggcc tcttgaaaac acttctttcc atttctttta
     9721 tcaggttctt atcagttgtc taatatctgg ccttctaact cattgcttag ctttcaccag
     9781 tttctttccc tgctttagat catcttgtgc atttttgtag acaatacttt cctaatatca
     9841 ctcatggtct tgggtgtcta tggagagagt gagagagaga gagattccct aaattggaga
     9901 caaaaaggaa aaaaaagttg ggtcacttct aggatcaaat ctagttctct tttatgaagg
     9961 ctcaaaagtt ttttccctct cagaatatgc taaacttcaa atgtgatctc tgaaaccatc
    10021 cgcaagtctg agatacgcca tggttttttg ggttttgcta gcaattctgg gcagagatcc
    10081 tggttgtcct tcaacattct acttttctgc catattatta gaattttagc ttggcacatg
    10141 atcacccaga atagactaat gtatttcatc aattctttat aaaccactaa gaaataatac
    10201 ctaagatagg gagtctaaca ggttaccact gcataaatat gggacaccaa ggtttatacc
    10261 ttcagagcaa ggataaagga gaaatcttcc atgaagaaag agggagcaaa gtgtaggggt
    10321 gggttgcccc tacacacctg tgggtgtttc tcgtaaggtg ggacgagaga tttggaaaag
    10381 aaaaagacac agagacaaag tatagagaaa gaaataaggg gaaccgggga accagcgttc
    10441 agcatatgga ggatcccgcc agcctctgag ttcccttagt atttattgat catctgtggg
    10501 tgtttctcaa agagggggat gtgtcagggt cacaagacaa ttgtggggag agggtcagca
    10561 gacaaacacg tgaacaaagg tcttggcatc atagacaatg taaaggatta agtgctgtgc
    10621 ttttagatat gcatacacat aaacatctca gtgctttaca aagcagtatt gctgcccgca
    10681 ggtcccacct ccagccctaa ggcggttttt ccctatctca gtagatggag catacaatcg
    10741 ggttttatac cgagacattc cattgcccag ggacaggcag gagacagatg ccttcctctt
    10801 gtctcaactg caagaggcat tccttcctct tttactaatc ctcctcagca cagacccttt
    10861 acgggtgtcg ggctggggga cggtcaggtc tttcccttcc cacgaggcca tatttcagac
    10921 tatcacatgg ggagaaacct tggacaatac ctggctttcc taggcagagg tccctgcggc
    10981 cttccgcagt ttttgtgtcc ctgggtactt gagattaggg agtggtgatg actcttaagg
    11041 agcatgctgc cttcaagcat ctgtttaaca aagcacatcc tgcaccgccc ttaatccatt
    11101 caactctgag ttgacacagc acatgtttca gagagcacgg ggttgggggt aaggtcacag
    11161 aatctcaagg cagaagaatt tttcttagta cataacaaaa tggagtctcc tatgtctact
    11221 tctttctaca cagacacagt aacaatctga tctctcttgc ttttccccac agcaaagcaa
    11281 tttgcatgct cagccttggc actggttgga aggagggact gccagtgaga atttgaacca
    11341 taaaccagta cacatgtagg cttgtaatct gaattcacac aaatggtatc atcctaaaga
    11401 gctcaagcag aaaatgtaat taaagtgagg ttagtattgc ccctagtgac tggcagaagc
    11461 atatgcaaat cctctatgga agaactcaac ttcaattcag gcagacaact tagtatctca
    11521 attaaaaaga tgtgataatg tgaaaaatag gtgttacaga attgatgact acaatgtatc
    11581 tcccatcctt gaagctcaca ccctcccagc taggggtagc catgtgactg agttttgact
    11641 aatgggatat aaaaggaagt actggccggg tgcggtgtct cacgcctgta atctcagcac
    11701 tttgggaggc caaggcgggt ggatcacctg aggtcaggag ttcgagacca gcctggccaa
    11761 gatggtgaaa ccccgtctct actcaaaata caaaaaagta gctgggcgtg gtggcacaca
    11821 cttgtgatcc cagctactca ggaggctgag gcaagagaat cgcttgaacc cgggagacgg
    11881 aggttgcagt gagctgagat tgcaccactg cactccaacc tgggtgacaa agcgagacct
    11941 tgtctcaaaa aaaaaaaaaa aaaaaggaag tactgtgtgt ggttgcttct gggaaccttc
    12001 ctttcttgca gctgtagcat tctgtttgcc tcttctttct atttctctgt tccatcctgc
    12061 tgcctggaag cagatgctgc cgtttacacc tgaacataag ggttgcacag ggggatggta
    12121 gagtagtggg ctgcaggggc tgattcttgg ggctttgtaa aacagagaca tatcagcact
    12181 ggcctgcaca ctttgagact ttcacaagaa acaaattaaa aaattgttta agccatagtt
    12241 acttgggttt tcctattaat tatagctgaa tcctagctga taaaggacat aaagattatt
    12301 aagacatgtt tgtcctcaaa aaatggagag ccaccccagg gacagtttct attcaatgta
    12361 gggagggcta taatagatga atatacaaag tgggagtcta gggagaaatc gtctggtaga
    12421 ggagggcaaa gagaaatcta ggaagatgtt tttaaaaata tggaatcctt gaacaggacc
    12481 tggttttaaa ggtttacttc agaattgaac atattttttt agtctagacc tttaggggaa
    12541 aaacttaaag ctttcaggac tcaaagacaa gttactgttc ccatggctaa attgcattcc
    12601 atatttattt gacaaatact tattgcaggt cgggtactgt tttagccact ggagatgcag
    12661 taagaaactt aattaatttt tttttttttt gagacagagt cttgctctgt cacccaggtt
    12721 ggaatgcagt gacatgataa cagcttgctg cagccttgac ctccgtggct caggccatac
    12781 tcccatctca gcttcccaag tagctaagac tacaggtctg ccccaccacc accagctaat
    12841 ttttaaattt tttgtggaga ctggagtctc actaagttgc ccaggttggc ctcaaactcc
    12901 tggcctcaag ccatcctccc acttcggcct ctcaaattgc tgggattata ggcatgagac
    12961 accacgcctg gcccaacaaa gttttaaaaa tcactgtatt tatgaagttt acattctagt
    13021 tgtggaaacc gacactaaac aaatagacat ttcagtggta agtgttacaa aggaaagaga
    13081 caacagcata agggaatgga acatgaaggt aggggacact tttagaataa gatgatccga
    13141 gaaggctcct ctgaggaagt gacatttaac ctgaaacctt aatgatacga aggatcaaaa
    13201 ctattttaaa aattcggaag tggaggtggg ggagagaagt tctaggcaaa gggaggaaat
    13261 agaagctgat ggctctccac caggaaacag tggatgaaga atggagaaga attgtagggc
    13321 ccaagatcag agagatgagt agggccagat catgtgggca agcttttatg tattagcacg
    13381 cttagaatta atattctgct tgagaactaa cctgatattt gaaacagagt gaaaaagaaa
    13441 atacctccca cagtatactt catagcttct tatttagccc aagctgtcag ggaaataaag
    13501 tccagctatg gctaagtttc acataatgca ctgctttgga gtttttgtct catctgttag
    13561 agtaagataa gtaccacata ctattataag gctatatagc ataaaatgat gatgcatttt
    13621 ctcctgtcat tgataagagg atttttttaa aagtaaattt cttggtttag aatttgaaag
    13681 cagtttcaaa cttggataac acaattcaga tcttttttta aaattatttt ttatttatat
    13741 taaaaaaaaa tttttttttt gagatagggt ctggctctat cacgcagact ggcacacagt
    13801 ggcacaatct tggctcattg caacctctgc ctcccaggct gaagccatcc tcccacctca
    13861 gcctcccaag tagctgggac tacaggcatg caccaccatg cctggctaat ttttgtagag
    13921 agggggtctc actttgttac ccagactggg agatttttct gtacttggct gtatgctttc
    13981 cattgatctg ctgctgtttt cactattatt ccttcttttg tgtgggtatt ttcttttgat
    14041 ttttagaatt ttacttgtaa acatgctaca tatacatata atttggtgcc ctgtttttcc
    14101 atttacatat tatactttcc atattagtac attgtctgat atttctgcat aatactcaat
    14161 tcaacttacc tgctattgtt tttatcatat ctctattaca aacattaggc ccatttccct
    14221 tttttaaaag aggaaagttt gaggttatgg gcggtgttct gataaaataa ataatatttt
    14281 gtcatgtttg ttttttcatt gattaggaaa cattgttttg aatttaatgc ctcaaatttg
    14341 gtgcacaata atttgaaggg aaaaactgac aagtttgaaa tgaacattga tattacataa
    14401 tttacattaa aatgatacat cttttgtaaa tttgtaagaa gaataaataa atgaaaacct
    14461 gattaccttt
//
