LOCUS       NM_019896               1097 bp    mRNA    linear   PRI 01-OCT-2024
DEFINITION  Homo sapiens DNA polymerase epsilon 4, accessory subunit (POLE4),
            mRNA.
ACCESSION   NM_019896
VERSION     NM_019896.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Hill,B.R., Ozgencil,M., Buckley-Benbow,L., Skingsley,S.L.P.,
            Tomlinson,D., Eizmendi,C.O., Agnarelli,A. and Bellelli,R.
  TITLE     Loss of POLE3-POLE4 unleashes replicative gap accumulation upon
            treatment with PARP inhibitors
  JOURNAL   Cell Rep 43 (5), 114205 (2024)
   PUBMED   38753485
  REMARK    GeneRIF: Loss of POLE3-POLE4 unleashes replicative gap accumulation
            upon treatment with PARP inhibitors.
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1097)
  AUTHORS   Zahurancik,W.J. and Suo,Z.
  TITLE     Kinetic investigation of the polymerase and exonuclease activities
            of human DNA polymerase epsilon holoenzyme
  JOURNAL   J Biol Chem 295 (50), 17251-17264 (2020)
   PUBMED   33051204
REFERENCE   4  (bases 1 to 1097)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1097)
  AUTHORS   Bellelli,R., Belan,O., Pye,V.E., Clement,C., Maslen,S.L.,
            Skehel,J.M., Cherepanov,P., Almouzni,G. and Boulton,S.J.
  TITLE     POLE3-POLE4 Is a Histone H3-H4 Chaperone that Maintains Chromatin
            Integrity during DNA Replication
  JOURNAL   Mol Cell 72 (1), 112-126 (2018)
   PUBMED   30217558
  REMARK    GeneRIF: iochemical analyses establish that POLE3-POLE4 is a
            histone chaperone that promotes tetrasome formation and DNA
            supercoiling in vitro. In cells, POLE3-POLE4 binds both newly
            synthesized and parental histones, and its depletion hinders
            helicase unwinding and chromatin PCNA unloading and compromises
            coordinated parental histone retention and new histone deposition
REFERENCE   6  (bases 1 to 1097)
  AUTHORS   Jager,S., Cimermancic,P., Gulbahce,N., Johnson,J.R., McGovern,K.E.,
            Clarke,S.C., Shales,M., Mercenne,G., Pache,L., Li,K., Hernandez,H.,
            Jang,G.M., Roth,S.L., Akiva,E., Marlett,J., Stephens,M., D'Orso,I.,
            Fernandes,J., Fahey,M., Mahon,C., O'Donoghue,A.J., Todorovic,A.,
            Morris,J.H., Maltby,D.A., Alber,T., Cagney,G., Bushman,F.D.,
            Young,J.A., Chanda,S.K., Sundquist,W.I., Kortemme,T.,
            Hernandez,R.D., Craik,C.S., Burlingame,A., Sali,A., Frankel,A.D.
            and Krogan,N.J.
  TITLE     Global landscape of HIV-human protein complexes
  JOURNAL   Nature 481 (7381), 365-370 (2011)
   PUBMED   22190034
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1097)
  AUTHORS   Wang,Y.L., Faiola,F., Xu,M., Pan,S. and Martinez,E.
  TITLE     Human ATAC Is a GCN5/PCAF-containing acetylase complex with a novel
            NC2-like histone fold module that interacts with the TATA-binding
            protein
  JOURNAL   J Biol Chem 283 (49), 33808-33815 (2008)
   PUBMED   18838386
REFERENCE   8  (bases 1 to 1097)
  AUTHORS   Li,Y., Pursell,Z.F. and Linn,S.
  TITLE     Identification and cloning of two histone fold motif-containing
            subunits of HeLa DNA polymerase epsilon
  JOURNAL   J Biol Chem 275 (30), 23247-23252 (2000)
   PUBMED   10801849
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BU594966.1, BC031331.1 and AC007400.4.
            
            On May 17, 2019 this sequence version replaced NM_019896.3.
            
            Summary: POLE4 is a histone-fold protein that interacts with other
            histone-fold proteins to bind DNA in a sequence-independent manner.
            These histone-fold protein dimers combine within larger enzymatic
            complexes for DNA transcription, replication, and
            packaging.[supplied by OMIM, Apr 2004].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7410570.204463.1,
                                           SRR7410570.204464.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000483063.2/ ENSP00000420176.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                BU594966.1         4-13
            11-86               BC031331.1         1-76
            87-87               AC007400.4         11564-11564
            88-701              BC031331.1         78-691
            702-1097            AC007400.4         22568-22963
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p12"
     gene            1..1097
                     /gene="POLE4"
                     /gene_synonym="p12; YHHQ1"
                     /note="DNA polymerase epsilon 4, accessory subunit"
                     /db_xref="GeneID:56655"
                     /db_xref="HGNC:HGNC:18755"
                     /db_xref="MIM:607269"
     exon            1..250
                     /gene="POLE4"
                     /gene_synonym="p12; YHHQ1"
                     /inference="alignment:Splign:2.1.0"
     CDS             38..391
                     /gene="POLE4"
                     /gene_synonym="p12; YHHQ1"
                     /EC_number="2.7.7.7"
                     /note="DNA polymerase II subunit 4; DNA polymerase epsilon
                     subunit p12; polymerase (DNA-directed), epsilon 4,
                     accessory subunit; polymerase (DNA) epsilon 4, accessory
                     subunit"
                     /codon_start=1
                     /product="DNA polymerase epsilon subunit 4"
                     /protein_id="NP_063949.2"
                     /db_xref="CCDS:CCDS1957.1"
                     /db_xref="GeneID:56655"
                     /db_xref="HGNC:HGNC:18755"
                     /db_xref="MIM:607269"
                     /translation="MAAAAAAGSGTPREEEGPAGEAAASQPQAPTSVPGARLSRLPLA
                     RVKALVKADPDVTLAGQEAIFILARAAELFVETIAKDAYCCAQQGKRKTLQRRDLDNA
                     IEAVDEFAFLEGTLD"
     exon            251..335
                     /gene="POLE4"
                     /gene_synonym="p12; YHHQ1"
                     /inference="alignment:Splign:2.1.0"
     exon            336..377
                     /gene="POLE4"
                     /gene_synonym="p12; YHHQ1"
                     /inference="alignment:Splign:2.1.0"
     exon            378..1097
                     /gene="POLE4"
                     /gene_synonym="p12; YHHQ1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      678..683
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLE4"
                     /gene_synonym="p12; YHHQ1"
                     /note="hexamer: AATAAA"
     polyA_site      701
                     /gene="POLE4"
                     /gene_synonym="p12; YHHQ1"
                     /note="major polyA site"
     regulatory      903..908
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLE4"
                     /gene_synonym="p12; YHHQ1"
                     /note="hexamer: AATAAA"
     polyA_site      930
                     /gene="POLE4"
                     /gene_synonym="p12; YHHQ1"
ORIGIN      
        1 acagtcggcg gccgcgcgca gcacgctcaa ggccgggatg gcggcggcgg cggcggcagg
       61 aagcgggacg ccccgagagg aggagggacc tgctggggag gcagcggcct cgcagcccca
      121 ggccccaacg agtgtgcctg gggctcgtct ctcgaggttg cctctggcgc gagtgaaggc
      181 cttggtgaag gcagatcccg acgtgacgct agcgggacag gaagccatct tcattctggc
      241 acgagccgcg gaactgtttg tggagaccat tgcaaaagat gcctactgtt gcgctcagca
      301 gggaaaaagg aaaacccttc agaggagaga cttggataat gcaatagaag ctgtggatga
      361 atttgctttt ctggaaggta ctttagattg attgccgagc ggggcagttt tgtgagcctt
      421 catctgaagc cttcagttca cccctctgca caggcctcag ctttgaagaa cggagtcttt
      481 gcacttacac acactcttcc tgttctgcct tcacctatgc cgggataagc agagatctca
      541 tcaattagct cttctctgca aggtcttcca ctatttctgt ctgtcttcca tatcaagcct
      601 ggatgcagct gctgctgctt agagcagaga tgaagaaagt gttctgcata agtggcttcc
      661 tgaatgatga ggaccagaat aaaggttttt gatcaacctc agtccagtgt gttttataat
      721 ctcgcctttg ttcctaccct gtgactggag agtcaccata cctggtcaca gaccttcctg
      781 atcttccctg ccagccattc taacatacag tcctttcatt aattttgttc ttttattttt
      841 ccagcacatc accagaaagt ggttccagaa tttatggaac accacttgcc ctcaggagtt
      901 caaataaata tggtcctaga ttgaaagcca gttaaatttt ttttaagttt tatttattga
      961 catatccaca tactctatat ttcaccaatt taaagtgtac aattcagtgg tatttagtat
     1021 attcacaaat atgtacacca gtcatcacag tcgattttag aacattttat ttatttattt
     1081 attattatta tacttta
//
