LOCUS       NM_017443               2194 bp    mRNA    linear   PRI 01-OCT-2024
DEFINITION  Homo sapiens DNA polymerase epsilon 3, accessory subunit (POLE3),
            transcript variant 1, mRNA.
ACCESSION   NM_017443
VERSION     NM_017443.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Hill,B.R., Ozgencil,M., Buckley-Benbow,L., Skingsley,S.L.P.,
            Tomlinson,D., Eizmendi,C.O., Agnarelli,A. and Bellelli,R.
  TITLE     Loss of POLE3-POLE4 unleashes replicative gap accumulation upon
            treatment with PARP inhibitors
  JOURNAL   Cell Rep 43 (5), 114205 (2024)
   PUBMED   38753485
  REMARK    GeneRIF: Loss of POLE3-POLE4 unleashes replicative gap accumulation
            upon treatment with PARP inhibitors.
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2194)
  AUTHORS   Zahurancik,W.J. and Suo,Z.
  TITLE     Kinetic investigation of the polymerase and exonuclease activities
            of human DNA polymerase epsilon holoenzyme
  JOURNAL   J Biol Chem 295 (50), 17251-17264 (2020)
   PUBMED   33051204
REFERENCE   4  (bases 1 to 2194)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2194)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2194)
  AUTHORS   Kukimoto,I., Elderkin,S., Grimaldi,M., Oelgeschlager,T. and
            Varga-Weisz,P.D.
  TITLE     The histone-fold protein complex CHRAC-15/17 enhances nucleosome
            sliding and assembly mediated by ACF
  JOURNAL   Mol Cell 13 (2), 265-277 (2004)
   PUBMED   14759371
REFERENCE   7  (bases 1 to 2194)
  AUTHORS   Collins,N., Poot,R.A., Kukimoto,I., Garcia-Jimenez,C., Dellaire,G.
            and Varga-Weisz,P.D.
  TITLE     An ACF1-ISWI chromatin-remodeling complex is required for DNA
            replication through heterochromatin
  JOURNAL   Nat Genet 32 (4), 627-632 (2002)
   PUBMED   12434153
REFERENCE   8  (bases 1 to 2194)
  AUTHORS   Bolognese,F., Imbriano,C., Caretti,G. and Mantovani,R.
  TITLE     Cloning and characterization of the histone-fold proteins YBL1 and
            YCL1
  JOURNAL   Nucleic Acids Res 28 (19), 3830-3838 (2000)
   PUBMED   11000277
REFERENCE   9  (bases 1 to 2194)
  AUTHORS   Li,Y., Pursell,Z.F. and Linn,S.
  TITLE     Identification and cloning of two histone fold motif-containing
            subunits of HeLa DNA polymerase epsilon
  JOURNAL   J Biol Chem 275 (30), 23247-23252 (2000)
   PUBMED   10801849
REFERENCE   10 (bases 1 to 2194)
  AUTHORS   Poot,R.A., Dellaire,G., Hulsmann,B.B., Grimaldi,M.A., Corona,D.F.,
            Becker,P.B., Bickmore,W.A. and Varga-Weisz,P.D.
  TITLE     HuCHRAC, a human ISWI chromatin remodelling complex contains hACF1
            and two novel histone-fold proteins
  JOURNAL   EMBO J 19 (13), 3377-3387 (2000)
   PUBMED   10880450
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA908447.1, AK074762.1 and AF070640.1.
            
            On Nov 22, 2018 this sequence version replaced NM_017443.4.
            
            Summary: POLE3 is a histone-fold protein that interacts with other
            histone-fold proteins to bind DNA in a sequence-independent manner.
            These histone-fold protein dimers combine within larger enzymatic
            complexes for DNA transcription, replication, and
            packaging.[supplied by OMIM, Apr 2004].
            
            Transcript Variant: This variant (1) represents the longer
            protein-coding transcript. Variants 1 and 2 both encode the same
            protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.96638.1, AK074762.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1968189 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374171.5/ ENSP00000363286.4
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-71                DA908447.1         75-145
            72-422              DA908447.1         147-497
            423-2188            AK074762.1         420-2185
            2189-2194           AF070640.1         1584-1589
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q32"
     gene            1..2194
                     /gene="POLE3"
                     /gene_synonym="CHARAC17; CHRAC17; CHRAC2; p17; YBL1"
                     /note="DNA polymerase epsilon 3, accessory subunit"
                     /db_xref="GeneID:54107"
                     /db_xref="HGNC:HGNC:13546"
                     /db_xref="MIM:607267"
     exon            1..59
                     /gene="POLE3"
                     /gene_synonym="CHARAC17; CHRAC17; CHRAC2; p17; YBL1"
                     /inference="alignment:Splign:2.1.0"
     exon            60..240
                     /gene="POLE3"
                     /gene_synonym="CHARAC17; CHRAC17; CHRAC2; p17; YBL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             175..618
                     /gene="POLE3"
                     /gene_synonym="CHARAC17; CHRAC17; CHRAC2; p17; YBL1"
                     /EC_number="2.7.7.7"
                     /note="polymerase (DNA) epsilon 3, accessory subunit;
                     polymerase (DNA directed), epsilon 3, accessory subunit;
                     histone fold protein CHRAC17; arsenic transactivated
                     protein; DNA polymerase epsilon p17 subunit; asTP;
                     CHRAC-17; huCHRAC17; DNA polymerase II subunit 3; DNA
                     polymerase epsilon subunit p17; chromatin accessibility
                     complex 17 kDa protein; polymerase (DNA directed), epsilon
                     3 (p17 subunit); chromatin accessibility complex subunit
                     2"
                     /codon_start=1
                     /product="DNA polymerase epsilon subunit 3"
                     /protein_id="NP_059139.3"
                     /db_xref="CCDS:CCDS6795.1"
                     /db_xref="GeneID:54107"
                     /db_xref="HGNC:HGNC:13546"
                     /db_xref="MIM:607267"
                     /translation="MAERPEDLNLPNAVITRIIKEALPDGVNISKEARSAISRAASVF
                     VLYATSCANNFAMKGKRKTLNASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEA
                     SEQKKKDKDKKTDSEEQDKSRDEDNDEDEERLEEEEQNEEEEVDN"
     exon            241..326
                     /gene="POLE3"
                     /gene_synonym="CHARAC17; CHRAC17; CHRAC2; p17; YBL1"
                     /inference="alignment:Splign:2.1.0"
     exon            327..445
                     /gene="POLE3"
                     /gene_synonym="CHARAC17; CHRAC17; CHRAC2; p17; YBL1"
                     /inference="alignment:Splign:2.1.0"
     exon            446..2194
                     /gene="POLE3"
                     /gene_synonym="CHARAC17; CHRAC17; CHRAC2; p17; YBL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2174..2179
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLE3"
                     /gene_synonym="CHARAC17; CHRAC17; CHRAC2; p17; YBL1"
                     /note="hexamer: AATAAA"
     polyA_site      2194
                     /gene="POLE3"
                     /gene_synonym="CHARAC17; CHRAC17; CHRAC2; p17; YBL1"
                     /note="major polyA site"
ORIGIN      
        1 agcagcggcg cagggcacca tgggaaacgg acggaagctc cattgagcca aataagttgg
       61 ccacgtgggg cggaacggaa acctcgcagg gtcagaccgt agcgacgcgg gaagtccgga
      121 cgcagtagct ccctgaagcg gaggcgaagg ggagtttaag ccccagcggc ggcaatggcg
      181 gagaggcccg aggacctaaa cctgcccaat gccgtgatca ccaggatcat caaggaggcg
      241 ctcccggacg gtgtcaacat ctccaaggag gcccggagcg ccatctcccg cgccgccagc
      301 gtcttcgtgc tgtacgccac atcctgtgct aacaactttg caatgaaagg aaagcggaag
      361 acgctgaatg ccagtgatgt gctctcagcc atggaagaga tggagttcca gcggttcgtt
      421 accccattga aagaagctct ggaagcatat aggcgggagc agaaaggcaa gaaggaggcc
      481 tcagagcaaa agaagaagga caaagacaaa aaaacagact cggaagagca agacaagagc
      541 agggatgagg acaatgatga agacgaagaa aggctggaag aagaagaaca gaatgaagag
      601 gaagaagtag acaactgaaa agggcgggag actgtggcac cttggaaggt accacttgaa
      661 atgagacgtg ctacgtgttt ctgtgaagct tttccatgct gggcagagta gtctcaggca
      721 aaagtgcctg aaaagatcag aataaaaact gactagaaat accgtcaaaa ccagcactta
      781 ccaaactcag agcatctgag tagcagaatc ctgttttgct tcatcaatct gaaggttatg
      841 gccttttgac aagagggatt ctgaacgact aaattagtca gtttgctcgc ttttgtttac
      901 ttaaatgtat ggttagatgg tttttgatct gcagtcttcc ctagtccccc aaataagtaa
      961 taataacttc catgctgcct gctgtgttcc agatcacaga ggcagccaag gctcaggaaa
     1021 aactggggct ttgcttatgg tcagcctatg tttgattgca cagtctttga caatagtagc
     1081 ttactaagac tgctcagaat gagtaagctt taggaaacta ctaaaatgct gtgggagaat
     1141 aacaaaatcc gttctctctc ttcacttgac agaagcatta actgaatttt gtttttcata
     1201 gacccacttg gcccaaaacc tttctatatt gcctgtagat gatcggcctg ctggtgtggt
     1261 agtaatctga tcacagatat tcttaaatca gcagtgtgga atggtattag tcaaacatgg
     1321 tgccagtgtc ttttacaaaa acagatggca tagtgtcaga tgtaccttac tctctgctac
     1381 tgtacagctt taggatttgt catcacctcc cggaaggtgt aaaaccccag gcttcctctt
     1441 cttgtagtcc atctcccaga cttgtgacta tggcttttca ttcttcaatt catgacgagt
     1501 gccaaacttg attttctcta ggcacagctt tctccctctg tgcctcctgc ttcctttctc
     1561 tctcctgcct ctcctctgct ccccatccca ctttctcatc tgcctccttt tctcacttct
     1621 gtcagtctgt aagctttgat aacctgctta atactccaaa gtgtgagttc ctctgatctc
     1681 ttgattcctt agttctaatc tcacgttttg tttttaagag atggagtctc tcactctgtg
     1741 gcccaggctg gagtgcagtg gcatgatcat agctcattgc atccttgaaa tcctgggctc
     1801 aggtgatcct cccgcctgag cctcctgagt atctgggact acagatgcgt gccaccaagc
     1861 ctggctaatt ttgtctcatg tcttctaaaa attattttgt gaagcccctt cacaaaaaac
     1921 cttaagggaa atctgatggt gctcaggaat ctaactctcc ctaaaccatc ctctttaact
     1981 gcttctaaaa tatctctgtt ggcctttctt agcctttttc tgtttccatt cagtgctcca
     2041 agcgcttttt gtttctaagt tgagtgcttg gggtttgaca ggtagtgacg tgtagtttga
     2101 cactgttaac ttgttaaata cagtgaaaag tttgtgagtg aagaatgctg agaagattgt
     2161 aatgctttgt acaaataaaa gtcttgttaa aaaa
//
