LOCUS       NM_002692               1692 bp    mRNA    linear   PRI 06-AUG-2024
DEFINITION  Homo sapiens DNA polymerase epsilon 2, accessory subunit (POLE2),
            transcript variant 1, mRNA.
ACCESSION   NM_002692
VERSION     NM_002692.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Jian,H., Chen,Z.Q., Du,H., Liao,T., Sun,Y.C., Ke,D. and Yu,Y.
  TITLE     Inhibition of ferroptosis by POLE2 in gastric cancer cells involves
            the activation of NRF2/GPX4 pathway
  JOURNAL   J Cell Mol Med 28 (1), e17983 (2024)
   PUBMED   38070189
  REMARK    GeneRIF: Inhibition of ferroptosis by POLE2 in gastric cancer cells
            involves the activation of NRF2/GPX4 pathway.
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Jiang,D., Zhang,H., Yin,B., He,M., Lu,X. and He,C.
  TITLE     The Prognostic Hub Gene POLE2 Promotes BLCA Cell Growth via the
            PI3K/AKT Signaling Pathway
  JOURNAL   Comb Chem High Throughput Screen 27 (13), 1984-1998 (2024)
   PUBMED   38963027
  REMARK    GeneRIF: The Prognostic Hub Gene POLE2 Promotes BLCA Cell Growth
            via the PI3K/AKT Signaling Pathway.
REFERENCE   3  (bases 1 to 1692)
  AUTHORS   Sun,M.Y., Wang,L. and Shen,Z.Y.
  TITLE     POLE2 Regulates Apoptosis of Oral Squamous Cell Carcinoma Cells
            through the PI3K/AKT Signaling Pathway
  JOURNAL   Curr Med Sci 43 (6), 1162-1172 (2023)
   PUBMED   38079056
  REMARK    GeneRIF: POLE2 Regulates Apoptosis of Oral Squamous Cell Carcinoma
            Cells through the PI3K/AKT Signaling Pathway.
REFERENCE   4  (bases 1 to 1692)
  AUTHORS   Guo,H., Ren,H., Han,K., Li,J., Dong,Y., Zhao,X. and Li,C.
  TITLE     Knockdown of HDAC10 inhibits POLE2-mediated DNA damage repair in
            NSCLC cells by increasing SP1 acetylation levels
  JOURNAL   Pulm Pharmacol Ther 83, 102250 (2023)
   PUBMED   37657752
  REMARK    GeneRIF: Knockdown of HDAC10 inhibits POLE2-mediated DNA damage
            repair in NSCLC cells by increasing SP1 acetylation levels.
REFERENCE   5  (bases 1 to 1692)
  AUTHORS   Huang,D., Jokela,M., Tuusa,J., Skog,S., Poikonen,K. and
            Syvaoja,J.E.
  TITLE     E2F mediates induction of the Sp1-controlled promoter of the human
            DNA polymerase epsilon B-subunit gene POLE2
  JOURNAL   Nucleic Acids Res 29 (13), 2810-2821 (2001)
   PUBMED   11433027
REFERENCE   6  (bases 1 to 1692)
  AUTHORS   Li,Y., Pursell,Z.F. and Linn,S.
  TITLE     Identification and cloning of two histone fold motif-containing
            subunits of HeLa DNA polymerase epsilon
  JOURNAL   J Biol Chem 275 (30), 23247-23252 (2000)
   PUBMED   10801849
REFERENCE   7  (bases 1 to 1692)
  AUTHORS   Jokela,M., Makiniemi,M., Lehtonen,S., Szpirer,C., Hellman,U. and
            Syvaoja,J.E.
  TITLE     The small subunits of human and mouse DNA polymerase epsilon are
            homologous to the second largest subunit of the yeast Saccharomyces
            cerevisiae DNA polymerase epsilon
  JOURNAL   Nucleic Acids Res 26 (3), 730-734 (1998)
   PUBMED   9443964
REFERENCE   8  (bases 1 to 1692)
  AUTHORS   Li,Y., Asahara,H., Patel,V.S., Zhou,S. and Linn,S.
  TITLE     Purification, cDNA cloning, and gene mapping of the small subunit
            of human DNA polymerase epsilon
  JOURNAL   J Biol Chem 272 (51), 32337-32344 (1997)
   PUBMED   9405441
REFERENCE   9  (bases 1 to 1692)
  AUTHORS   Wood,R.D. and Shivji,M.K.
  TITLE     Which DNA polymerases are used for DNA-repair in eukaryotes?
  JOURNAL   Carcinogenesis 18 (4), 605-610 (1997)
   PUBMED   9111189
  REMARK    Review article
REFERENCE   10 (bases 1 to 1692)
  AUTHORS   Wang,T.S., Hu,S.Z. and Korn,D.
  TITLE     DNA primase from KB cells. Characterization of a primase activity
            tightly associated with immunoaffinity-purified DNA
            polymerase-alpha
  JOURNAL   J Biol Chem 259 (3), 1854-1865 (1984)
   PUBMED   6693436
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC126218.1 and BG189206.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_002692.3.
            
            Summary: DNA polymerase epsilon, which is involved in DNA repair
            and replication, is composed of a large catalytic subunit and a
            small accessory subunit. The protein encoded by this gene
            represents the small subunit (B). Defects in this gene have been
            linked to colorectal cancer and to combined immunodeficiency.
            [provided by RefSeq, Jan 2017].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK314963.1, AF036899.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000216367.10/ ENSP00000216367.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1628              BC126218.1         90-1717
            1629-1692           BG189206.1         125-188
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q21.3"
     gene            1..1692
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /note="DNA polymerase epsilon 2, accessory subunit"
                     /db_xref="GeneID:5427"
                     /db_xref="HGNC:HGNC:9178"
                     /db_xref="MIM:602670"
     exon            1..79
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     CDS             12..1595
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /EC_number="2.7.7.7"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     polymerase epsilon subunit 2; DNA polymerase epsilon
                     subunit B; DNA polymerase II subunit 2; polymerase (DNA
                     directed), epsilon 2 (p59 subunit); polymerase (DNA)
                     epsilon 2, accessory subunit; polymerase (DNA directed),
                     epsilon 2, accessory subunit"
                     /codon_start=1
                     /product="DNA polymerase epsilon subunit 2 isoform 1"
                     /protein_id="NP_002683.2"
                     /db_xref="CCDS:CCDS32073.1"
                     /db_xref="GeneID:5427"
                     /db_xref="HGNC:HGNC:9178"
                     /db_xref="MIM:602670"
                     /translation="MAPERLRSRALSAFKLRGLLLRGEAIKYLTEALQSISELELEDK
                     LEKIINAVEKQPLSSNMIERSVVEAAVQECSQSVDETIEHVFNIIGAFDIPRFVYNSE
                     RKKFLPLLMTNHPAPNLFGTPRDKAEMFRERYTILHQRTHRHELFTPPVIGSHPDESG
                     SKFQLKTIETLLGSTTKIGDAIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLY
                     TEACFVLAEGWFEDQVFHVNAFGFPPTEPSSTTRAYYGNINFFGGPSNTSVKTSAKLK
                     QLEEENKDAMFVFLSDVWLDQVEVLEKLRIMFAGYSPAPPTCFILCGNFSSAPYGKNQ
                     VQALKDSLKTLADIICEYPDIHQSSRFVFVPGPEDPGFGSILPRPPLAESITNEFRQR
                     VPFSVFTTNPCRIQYCTQEITVFREDLVNKMCRNCVRFPSSNLAIPNHFVKTILSQGH
                     LTPLPLYVCPVYWAYDYALRVYPVPDLLVIADKYDPFTTTNTECLCINPGSFPRSGFS
                     FKVFYPSNKTVEDSKLQGF"
     exon            80..180
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            181..256
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            257..334
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            335..428
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            429..503
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            504..587
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            588..644
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            645..693
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            694..766
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            767..939
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1029
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1084
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1144
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1222
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1331
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1508
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1576
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1577..1692
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1671..1676
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /note="hexamer: AATAAA"
     polyA_site      1692
                     /gene="POLE2"
                     /gene_synonym="DPE2"
                     /note="major polyA site"
ORIGIN      
        1 aaatcgcaaa tatggcgccg gagcggctgc ggagccgggc gctctccgcc ttcaagttgc
       61 ggggcttgct gctccgtggt gaagctatta agtacctcac agaagctctt cagtctatca
      121 gtgaattaga gcttgaagat aaactggaaa agataattaa tgcagttgag aagcaaccct
      181 tgtcatcaaa catgattgaa cgatctgtgg tggaagcagc agtccaggaa tgcagtcagt
      241 ctgttgatga aactatagag cacgttttca atatcatagg agcatttgat attccacgct
      301 ttgtgtacaa ttcagaaaga aaaaaatttc ttcctctgtt aatgaccaac caccctgcac
      361 caaatttatt tggaacacca agagataaag cagagatgtt tcgtgagcga tataccattt
      421 tgcaccagag gacccacagg catgaattat ttactcctcc ggtgataggt tctcaccctg
      481 atgaaagcgg aagcaaattc cagcttaaaa caatagaaac cttattgggt agtacaacca
      541 aaatcggaga tgcgattgtt cttggaatga taacgcagtt aaaagaggga aaattttttc
      601 tggaagatcc tactggaaca gtccaactag accttagtaa agctcagttc catagtggtt
      661 tatacacaga ggcatgcttt gtcttagcag aaggttggtt tgaagatcaa gtgtttcatg
      721 tcaatgcctt tggatttcca cccactgagc cctctagtac tactagggca tactatggaa
      781 atattaattt ttttggaggt ccttctaata catctgtgaa gacttctgca aaactaaaac
      841 agctagaaga ggagaataaa gatgctatgt ttgtgttttt atctgatgtt tggttggacc
      901 aggtggaagt attggaaaaa cttcgcataa tgtttgctgg ttattcacca gcacctccaa
      961 cctgctttat tctgtgtggt aatttttcat ctgcaccata tggaaaaaat caagttcaag
     1021 ctttgaaaga ttccctaaaa actttggcag atataatatg tgaataccca gatattcacc
     1081 aaagtagtcg ttttgtgttt gtacctggtc cagaggatcc tggatttggt tccatcttac
     1141 caaggccacc acttgctgaa agcatcacta atgaattcag acaaagggta ccattttcag
     1201 tttttactac taatccttgc agaattcagt actgtacaca ggaaattact gtcttccgtg
     1261 aagacttagt aaataaaatg tgcagaaact gcgtccgttt tcctagcagc aatttggcta
     1321 ttcctaatca ctttgtaaag actatcttat cccaaggaca tctgactccc ctacctcttt
     1381 atgtctgccc agtgtattgg gcatatgact atgctttgag agtgtatcct gtgcccgatc
     1441 tacttgtcat tgcagacaaa tatgatcctt tcactacgac aaataccgaa tgcctctgca
     1501 taaaccctgg ctcttttcca agaagtggat tttcattcaa agttttttat ccttctaata
     1561 agacagtaga agatagcaaa cttcaaggct tttgagattc ttaaagatca tctgaagaaa
     1621 attcatcagt tttctgctta actctatatc ttatgtgatt ctgatattac aataaaatta
     1681 tggtaaactt ta
//
